Standard name
Human Ortholog
Description Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.14 0.2 0 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.1 0 0.08 0.06 0.29 0.38 0.31 0.38 0.55 0.54 0.49 0 0 0 0 0.08 0 0 0 0 0 0 0
Nucleus 0.46 0.53 0.34 0.35 0.48 0.42 0.39 0.39 0.41 0.41 0.38 0.37 0.42 0.35 0.41 0.41 0.41 0.48 0.28 0.48 0.18 0.47 0.43 0.24
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.68 0.64 0.71 0.67 0.52 0.47 0.48 0.44 0.38 0.37 0.39 0.37 0.59 0.53 0.41 0.53 0.56 0.46 0.67 0.42 0.69 0.41 0.36 0.42
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.07 0.13 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 5 3 5 2 4 2 0 0 1 0 2 2 1 1 1 0 0 2
Bud Neck 0 0 0 0 0 0 0 1 0 0 1 1 1 0 0 0 0 0 0 0 1 0 0 1
Bud Site 0 0 0 0 2 4 6 16 12 14 15 15 0 0 1 0 1 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 2 7 5 12 7 17 10 9 5 10 11 16 32 60 6 7 10 0 0 0 1 0 1
Endoplasmic Reticulum 0 0 1 1 0 0 1 0 0 0 0 1 0 1 0 2 1 5 0 0 0 0 0 0
Endosome 0 0 5 0 4 4 3 26 7 3 2 7 1 6 7 3 10 2 0 0 8 1 2 6
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 1
Mitochondria 10 6 2 17 19 96 165 153 116 248 265 178 0 3 0 9 19 7 0 0 0 0 0 4
Nucleus 52 31 89 71 151 138 170 192 124 187 188 135 74 83 120 84 100 88 31 34 51 66 80 63
Nuclear Periphery 1 0 7 2 2 9 11 7 8 4 13 7 0 0 1 1 0 1 0 0 5 0 0 4
Nucleolus 76 38 182 137 163 156 208 217 115 169 193 134 105 125 121 110 135 83 76 30 195 59 66 110
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 4 0 3 9 7 22 10 18 8 15 1 9 12 3 4 3 1 1 6 3 4 9
Vac/Vac Membrane 0 0 4 3 8 5 4 6 9 7 6 11 2 2 1 9 15 8 2 2 7 10 24 52
Unique Cell Count 112 59 258 204 313 331 431 494 304 453 492 361 177 236 295 206 241 182 114 73 282 143 184 263
Labelled Cell Count 140 77 301 237 365 428 597 653 415 657 705 517 200 263 327 228 294 210 114 73 282 143 184 263


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 7.5 8.6 7.9 6.6 5.2 4.9 4.7 4.6 4.3 4.1 4.1 10.1 9.3 8.4 12.1 11.1 10.9 10.9 11.0 11.6
Std Deviation (1e-4) 1.1 1.4 1.8 1.6 1.5 1.3 1.7 1.4 1.2 1.2 1.3 0.9 2.6 2.4 2.2 3.2 3.4 3.4 2.8 2.5 2.3
Intensity Change (Log2) – – – -0.12 -0.39 -0.73 -0.81 -0.86 -0.91 -0.98 -1.06 -1.06 0.24 0.12 -0.02 0.5 0.38 0.35 0.34 0.37 0.44

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700051015WT3HU80HU120HU160051015WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3051015WT1AF100AF140AF180051015
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 0.7 -0.5 0.9 -0.6 0.2 0 -0.6 0.2 2.9 4.5 6.3 0.1 0.1 1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0.5 0.4 0 1.4 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.1 3.3 9.1 11.1 0 0 0 0 0 0 0 0 0 4.0 0
Nucleus 0.1 3.3 1.8 1.3 1.2 1.5 1.8 1.0 0.7 1.5 0.2 1.5 1.4 1.6 2.9
Nuclear Periphery 0 0 0 -0.1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.8 -4.5 -5.7 -5.7 0 0 0 0 0 -2.4 -4.0 -7.0 -3.8 -3.4 -5.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1.0 0 0 0 0 0 0 0 1.6 1.8 0 0 0
Vacuole 0 0.8 0 0 0 1.1 0 0 1.2 0 0 0 1.8 2.7 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.7557 7.2604 6.1188 5.8291 4.2351 5.9494 5.4182 7.6292 7.2658 6.6197 5.8761 6.3023 4.7848 6.3059 6.261 3.761 4.5477 5.1206
Actin 0.0151 0.0018 0.0134 0.001 0.0949 0.0047 0.018 0 0.0002 0 0.0026 0 0.0215 0 0.0083 0.0152 0 0.0002
Bud 0.0005 0.0001 0.0002 0.0003 0.0005 0.0002 0.0004 0.0001 0.0001 0.0001 0.0004 0.0001 0.0004 0.0002 0.0004 0.0009 0 0.0001
Bud Neck 0.0036 0.0004 0.0008 0.0048 0.0006 0.0012 0.0005 0.0002 0.0001 0.0003 0.0003 0.0006 0.0006 0.0002 0.0004 0.0003 0.0003 0.0005
Bud Periphery 0.0007 0.0002 0.0003 0.0008 0.0016 0.0004 0.0006 0.0001 0.0001 0.0002 0.0005 0.0002 0.0008 0.0004 0.0007 0.0013 0.0001 0.0002
Bud Site 0.0041 0.0007 0.0057 0.0051 0.001 0.0011 0.0012 0.0003 0.0001 0.0002 0.0007 0.0002 0.001 0.0004 0.001 0.0027 0.0001 0.0001
Cell Periphery 0.0004 0.0002 0.0002 0.0018 0.0007 0.0002 0.0004 0.0001 0.0001 0.0001 0.0002 0.0001 0.0004 0.0008 0.0002 0.0003 0.0001 0.0001
Cytoplasm 0.0086 0.0003 0.0089 0.0043 0.0008 0.0032 0.0023 0.0002 0.0001 0 0.0032 0 0.0051 0 0.002 0.0079 0 0.0005
Cytoplasmic Foci 0.0255 0.003 0.006 0.0113 0.0097 0.0042 0.0187 0.0029 0.0004 0 0.0048 0 0.0124 0.0001 0.004 0.0167 0.0001 0.0007
Eisosomes 0.0003 0 0.0002 0.0012 0.0022 0 0.0001 0 0 0 0 0 0.0005 0 0.0001 0.0003 0 0
Endoplasmic Reticulum 0.004 0.0001 0.0009 0.0007 0.022 0.001 0.0008 0 0.001 0 0.0032 0 0.0021 0 0.0012 0.0083 0 0.0002
Endosome 0.0264 0.0016 0.0047 0.0041 0.0132 0.0286 0.0079 0.0027 0.0137 0 0.0552 0 0.0083 0.0001 0.0084 0.1194 0 0.0075
Golgi 0.0057 0.0016 0.0042 0.0009 0.0229 0.009 0.0028 0.0004 0.0039 0 0.0044 0 0.0044 0 0.0015 0.0171 0 0.0039
Lipid Particles 0.014 0.0011 0.0046 0.0109 0.0099 0.0062 0.0081 0.0016 0.0003 0 0.0043 0 0.0127 0.0008 0.0019 0.0599 0.0003 0.008
Mitochondria 0.0034 0.0019 0.0052 0.0023 0.0843 0.0039 0.0033 0.0005 0.0036 0.0001 0.0071 0.0003 0.0038 0.0013 0.0099 0.0388 0.0005 0.0053
None 0.0104 0.0001 0.0062 0.0417 0.0015 0.0017 0.001 0.0001 0 0 0.0018 0 0.0126 0 0.0004 0.0032 0 0.0001
Nuclear Periphery 0.0103 0.0005 0.0014 0.0004 0.0029 0.0021 0.0107 0.0009 0.0065 0.0001 0.0575 0.0001 0.0171 0.0002 0.0119 0.0798 0.0005 0.0029
Nucleolus 0.4663 0.5559 0.6096 0.6317 0.3167 0.5989 0.5195 0.5779 0.5598 0.5636 0.4472 0.5081 0.5373 0.6022 0.6437 0.2092 0.6283 0.5977
Nucleus 0.3622 0.4235 0.3162 0.2635 0.3962 0.3239 0.3621 0.4088 0.4053 0.4347 0.3876 0.4894 0.3334 0.3865 0.2877 0.3724 0.3644 0.3571
Peroxisomes 0.0145 0.0034 0.0086 0.0046 0.0152 0.0012 0.0029 0.0004 0 0 0.001 0 0.0082 0.0001 0.0108 0.0039 0 0.0001
Punctate Nuclear 0.0187 0.0019 0.0019 0.0049 0.0009 0.0057 0.0328 0.0014 0.0002 0.0003 0.0064 0.0003 0.0128 0.0005 0.002 0.0183 0.0047 0.0112
Vacuole 0.0038 0.0013 0.0006 0.0033 0.0008 0.0017 0.0037 0.0009 0.0018 0.0003 0.004 0.0003 0.0023 0.0048 0.0017 0.0104 0.0002 0.0011
Vacuole Periphery 0.0016 0.0006 0.0003 0.0003 0.0015 0.0007 0.0023 0.0004 0.0025 0.0001 0.0075 0.0001 0.0021 0.0014 0.002 0.0139 0.0003 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 46.7941 35.1381 29.7988 24.3834 32.1554 26.1902 21.9936 21.4099 16.0931 26.5698
Translational Efficiency 2.0017 2.0354 2.2004 2.075 1.8313 1.8847 2.0754 1.3886 1.6417 1.9711

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
119 198 1964 1496 134 1757 82 1320 253 1955 2046 2816

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 954.42 1033.49 1140.62 1139.45 909.90 932.77 1240.58 1212.07 930.84 942.97 1144.63 1173.49
Standard Deviation 142.55 157.54 170.01 223.32 146.38 144.37 174.98 207.67 146.29 148.89 171.33 219.14
Intensity Change Log 2 — 0.114828 0.257122 0.255641 — 0.035813 0.447235 0.413693 — 0.076805 0.353039 0.334944

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000420 0.000331 0.000529 0.000991 0.000388 0.000325 0.001034 0.000635 0.000403 0.000325 0.000549 0.000824
Bud Neck 0.003389 0.005739 0.013887 0.029778 0.002322 0.005686 0.007011 0.025231 0.002824 0.005691 0.013611 0.027647
Bud Site 0.000642 0.000405 0.000815 0.003223 0.000501 0.000734 0.001280 0.003758 0.000567 0.000700 0.000834 0.003474
Cell Periphery 0.000079 0.000014 0.000021 0.000087 0.000081 0.000059 0.000035 0.000132 0.000080 0.000055 0.000021 0.000108
Cytoplasm 0.002789 0.000041 0.000138 0.001430 0.000492 0.001768 0.002662 0.000550 0.001573 0.001593 0.000239 0.001018
Cytoplasmic Foci 0.000055 0.000048 0.000322 0.000968 0.000196 0.001410 0.000828 0.001299 0.000130 0.001272 0.000343 0.001123
Eisosomes 0.000041 0.000038 0.000040 0.000046 0.000049 0.000042 0.000075 0.000053 0.000045 0.000042 0.000042 0.000050
Endoplasmic Reticulum 0.001892 0.001192 0.000927 0.001635 0.002202 0.001704 0.002939 0.001183 0.002056 0.001652 0.001007 0.001423
Endosome 0.000238 0.000120 0.000149 0.002460 0.000226 0.001395 0.000628 0.001868 0.000231 0.001266 0.000168 0.002183
Golgi 0.000185 0.000160 0.000427 0.001980 0.000412 0.001181 0.000450 0.001605 0.000306 0.001078 0.000428 0.001804
Lipid Particles 0.000072 0.000045 0.000173 0.000200 0.000182 0.000421 0.001076 0.000104 0.000130 0.000383 0.000209 0.000155
Mitochondria 0.000620 0.000571 0.001906 0.003278 0.000424 0.001973 0.000997 0.003380 0.000516 0.001831 0.001869 0.003326
Mitotic Spindle 0.001879 0.000974 0.004677 0.013584 0.000244 0.006205 0.015149 0.007829 0.001013 0.005675 0.005097 0.010886
None 0.008791 0.000458 0.000536 0.001481 0.003871 0.002276 0.001520 0.001063 0.006185 0.002092 0.000575 0.001285
Nuclear Periphery 0.000167 0.000811 0.000431 0.002296 0.000072 0.000455 0.000329 0.001177 0.000117 0.000491 0.000427 0.001772
Nuclear Periphery Foci 0.001314 0.002015 0.000298 0.002005 0.003424 0.001100 0.001136 0.000914 0.002431 0.001193 0.000332 0.001494
Nucleolus 0.464929 0.432980 0.481021 0.409826 0.392907 0.401538 0.536856 0.468975 0.426783 0.404723 0.483259 0.437552
Nucleus 0.510012 0.551692 0.491214 0.512726 0.588711 0.568166 0.412421 0.472588 0.551694 0.566497 0.488056 0.493912
Peroxisomes 0.002055 0.001840 0.001695 0.001658 0.002850 0.001376 0.010921 0.001973 0.002476 0.001423 0.002064 0.001806
Vacuole 0.000209 0.000254 0.000418 0.005684 0.000272 0.001220 0.002195 0.003158 0.000242 0.001122 0.000489 0.004500
Vacuole Periphery 0.000221 0.000272 0.000378 0.004662 0.000175 0.000965 0.000460 0.002525 0.000196 0.000895 0.000381 0.003660

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.11 -2.50 -3.16 -3.68 -2.29 0.86 -4.11 -2.70 -9.84 2.88 1.34 -2.64 -3.98 -5.59 -2.58
Bud Neck -2.03 -13.51 -19.25 -17.11 -11.54 -5.46 -3.72 -17.61 -15.95 -8.74 -5.35 -17.02 -26.23 -24.40 -13.64
Bud Site 0.88 -2.05 -6.22 -7.41 -5.86 -1.42 -2.88 -5.40 -5.12 -2.31 -0.96 -4.05 -8.17 -8.04 -6.99
Cell Periphery 1.99 1.66 -0.32 -7.18 -6.30 0.56 0.85 -0.60 -1.10 -1.30 0.93 2.12 -0.67 -1.74 -2.86
Cytoplasm 1.08 1.03 0.44 -5.71 -5.19 -2.02 -0.98 -0.77 1.77 0.95 -0.20 0.85 0.28 0.92 -1.91
Cytoplasmic Foci 0.37 -1.26 -3.53 -3.56 -1.95 -2.41 -1.00 -2.09 0.57 -0.58 -2.54 -0.90 -3.46 0.79 -2.32
Eisosomes 0.60 0.14 -1.06 -1.74 -2.82 1.62 -3.61 -0.52 -4.79 4.08 1.24 1.18 -0.92 -4.32 -4.59
Endoplasmic Reticulum 1.66 2.22 0.20 -2.82 -5.99 1.63 -1.42 2.58 2.53 3.38 1.65 3.72 1.91 0.57 -4.85
Endosome 1.69 1.14 -3.52 -3.74 -3.67 -4.22 -1.03 -3.01 -0.45 -1.81 -4.21 1.08 -4.63 -1.45 -4.79
Golgi 0.85 -1.72 -3.17 -3.22 -2.58 -1.97 -0.39 -1.73 -0.35 -1.64 -2.33 -0.69 -3.40 -1.04 -2.99
Lipid Particles 1.03 -0.94 -2.65 -3.03 -0.51 -1.17 -1.00 1.42 1.62 1.12 -1.44 -0.52 -0.72 1.27 0.22
Mitochondria 0.36 -3.12 -12.46 -12.79 -3.37 -3.85 -4.46 -5.14 -2.16 -3.71 -3.62 -3.47 -9.88 -3.71 -3.15
Mitotic Spindle 0.78 -2.95 -5.29 -6.27 -3.21 -4.60 -1.95 -5.57 -2.15 0.86 -3.61 -4.64 -7.70 -4.04 -2.97
None 1.76 1.72 1.51 -6.36 -5.56 0.85 1.16 1.41 2.29 0.37 1.67 2.27 1.96 1.60 -5.85
Nuclear Periphery -1.00 -3.21 -8.20 -2.13 -6.68 -5.96 -2.47 -4.46 -2.97 -2.25 -4.19 -4.05 -9.12 -6.51 -6.52
Nuclear Periphery Foci -0.59 2.82 -1.20 0.10 -4.28 1.52 1.44 1.66 0.75 0.85 1.46 2.48 1.21 -0.68 -4.42
Nucleolus 1.11 -0.41 2.83 2.01 7.95 -0.35 -3.12 -2.47 -4.82 1.90 1.36 -2.89 0.40 -2.03 6.65
Nucleus -1.48 0.83 -0.25 1.93 -2.60 0.90 4.65 4.76 9.07 -1.94 -0.90 3.75 3.14 8.60 -1.36
Peroxisomes 0.48 0.67 0.74 0.23 0.24 2.75 -3.80 1.68 -2.41 4.36 2.67 1.20 1.88 -1.79 1.47
Vacuole -0.80 -3.57 -6.21 -6.16 -5.83 -3.28 -1.90 -6.70 -3.89 0.13 -3.41 -3.69 -8.36 -6.01 -7.44
Vacuole Periphery -1.00 -5.10 -5.90 -5.82 -5.63 -3.28 -6.84 -5.99 -3.20 -5.09 -3.24 -8.44 -7.93 -5.38 -7.40
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1
Localization
Cell Percentages nucleolus (56%), nucleus (27%), mixed (16%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rrp8

Rrp8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rrp8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available