Standard name | |
---|---|
Human Ortholog | |
Description | Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 3 | 5 | 2 | 4 | 2 | 0 | 0 | 1 | 0 | 2 | 2 | 1 | 1 | 1 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 2 | 4 | 6 | 16 | 12 | 14 | 15 | 15 | 0 | 0 | 1 | 0 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 2 | 7 | 5 | 12 | 7 | 17 | 10 | 9 | 5 | 10 | 11 | 16 | 32 | 60 | 6 | 7 | 10 | 0 | 0 | 0 | 1 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 5 | 0 | 4 | 4 | 3 | 26 | 7 | 3 | 2 | 7 | 1 | 6 | 7 | 3 | 10 | 2 | 0 | 0 | 8 | 1 | 2 | 6 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 10 | 6 | 2 | 17 | 19 | 96 | 165 | 153 | 116 | 248 | 265 | 178 | 0 | 3 | 0 | 9 | 19 | 7 | 0 | 0 | 0 | 0 | 0 | 4 |
Nucleus | 52 | 31 | 89 | 71 | 151 | 138 | 170 | 192 | 124 | 187 | 188 | 135 | 74 | 83 | 120 | 84 | 100 | 88 | 31 | 34 | 51 | 66 | 80 | 63 |
Nuclear Periphery | 1 | 0 | 7 | 2 | 2 | 9 | 11 | 7 | 8 | 4 | 13 | 7 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 5 | 0 | 0 | 4 |
Nucleolus | 76 | 38 | 182 | 137 | 163 | 156 | 208 | 217 | 115 | 169 | 193 | 134 | 105 | 125 | 121 | 110 | 135 | 83 | 76 | 30 | 195 | 59 | 66 | 110 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 4 | 0 | 3 | 9 | 7 | 22 | 10 | 18 | 8 | 15 | 1 | 9 | 12 | 3 | 4 | 3 | 1 | 1 | 6 | 3 | 4 | 9 |
Vac/Vac Membrane | 0 | 0 | 4 | 3 | 8 | 5 | 4 | 6 | 9 | 7 | 6 | 11 | 2 | 2 | 1 | 9 | 15 | 8 | 2 | 2 | 7 | 10 | 24 | 52 |
Unique Cell Count | 112 | 59 | 258 | 204 | 313 | 331 | 431 | 494 | 304 | 453 | 492 | 361 | 177 | 236 | 295 | 206 | 241 | 182 | 114 | 73 | 282 | 143 | 184 | 263 |
Labelled Cell Count | 140 | 77 | 301 | 237 | 365 | 428 | 597 | 653 | 415 | 657 | 705 | 517 | 200 | 263 | 327 | 228 | 294 | 210 | 114 | 73 | 282 | 143 | 184 | 263 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.8 | 7.5 | 8.6 | 7.9 | 6.6 | 5.2 | 4.9 | 4.7 | 4.6 | 4.3 | 4.1 | 4.1 | 10.1 | 9.3 | 8.4 | 12.1 | 11.1 | 10.9 | 10.9 | 11.0 | 11.6 |
Std Deviation (1e-4) | 1.1 | 1.4 | 1.8 | 1.6 | 1.5 | 1.3 | 1.7 | 1.4 | 1.2 | 1.2 | 1.3 | 0.9 | 2.6 | 2.4 | 2.2 | 3.2 | 3.4 | 3.4 | 2.8 | 2.5 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.39 | -0.73 | -0.81 | -0.86 | -0.91 | -0.98 | -1.06 | -1.06 | 0.24 | 0.12 | -0.02 | 0.5 | 0.38 | 0.35 | 0.34 | 0.37 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.2 | 0.7 | -0.5 | 0.9 | -0.6 | 0.2 | 0 | -0.6 | 0.2 | 2.9 | 4.5 | 6.3 | 0.1 | 0.1 | 1.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 0.4 | 0 | 1.4 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.1 | 3.3 | 9.1 | 11.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 | 0 |
Nucleus | 0.1 | 3.3 | 1.8 | 1.3 | 1.2 | 1.5 | 1.8 | 1.0 | 0.7 | 1.5 | 0.2 | 1.5 | 1.4 | 1.6 | 2.9 |
Nuclear Periphery | 0 | 0 | 0 | -0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.8 | -4.5 | -5.7 | -5.7 | 0 | 0 | 0 | 0 | 0 | -2.4 | -4.0 | -7.0 | -3.8 | -3.4 | -5.3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 1.8 | 0 | 0 | 0 |
Vacuole | 0 | 0.8 | 0 | 0 | 0 | 1.1 | 0 | 0 | 1.2 | 0 | 0 | 0 | 1.8 | 2.7 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.7557 | 7.2604 | 6.1188 | 5.8291 | 4.2351 | 5.9494 | 5.4182 | 7.6292 | 7.2658 | 6.6197 | 5.8761 | 6.3023 | 4.7848 | 6.3059 | 6.261 | 3.761 | 4.5477 | 5.1206 |
Actin | 0.0151 | 0.0018 | 0.0134 | 0.001 | 0.0949 | 0.0047 | 0.018 | 0 | 0.0002 | 0 | 0.0026 | 0 | 0.0215 | 0 | 0.0083 | 0.0152 | 0 | 0.0002 |
Bud | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0.0009 | 0 | 0.0001 |
Bud Neck | 0.0036 | 0.0004 | 0.0008 | 0.0048 | 0.0006 | 0.0012 | 0.0005 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0006 | 0.0006 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0.0005 |
Bud Periphery | 0.0007 | 0.0002 | 0.0003 | 0.0008 | 0.0016 | 0.0004 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0008 | 0.0004 | 0.0007 | 0.0013 | 0.0001 | 0.0002 |
Bud Site | 0.0041 | 0.0007 | 0.0057 | 0.0051 | 0.001 | 0.0011 | 0.0012 | 0.0003 | 0.0001 | 0.0002 | 0.0007 | 0.0002 | 0.001 | 0.0004 | 0.001 | 0.0027 | 0.0001 | 0.0001 |
Cell Periphery | 0.0004 | 0.0002 | 0.0002 | 0.0018 | 0.0007 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0008 | 0.0002 | 0.0003 | 0.0001 | 0.0001 |
Cytoplasm | 0.0086 | 0.0003 | 0.0089 | 0.0043 | 0.0008 | 0.0032 | 0.0023 | 0.0002 | 0.0001 | 0 | 0.0032 | 0 | 0.0051 | 0 | 0.002 | 0.0079 | 0 | 0.0005 |
Cytoplasmic Foci | 0.0255 | 0.003 | 0.006 | 0.0113 | 0.0097 | 0.0042 | 0.0187 | 0.0029 | 0.0004 | 0 | 0.0048 | 0 | 0.0124 | 0.0001 | 0.004 | 0.0167 | 0.0001 | 0.0007 |
Eisosomes | 0.0003 | 0 | 0.0002 | 0.0012 | 0.0022 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.004 | 0.0001 | 0.0009 | 0.0007 | 0.022 | 0.001 | 0.0008 | 0 | 0.001 | 0 | 0.0032 | 0 | 0.0021 | 0 | 0.0012 | 0.0083 | 0 | 0.0002 |
Endosome | 0.0264 | 0.0016 | 0.0047 | 0.0041 | 0.0132 | 0.0286 | 0.0079 | 0.0027 | 0.0137 | 0 | 0.0552 | 0 | 0.0083 | 0.0001 | 0.0084 | 0.1194 | 0 | 0.0075 |
Golgi | 0.0057 | 0.0016 | 0.0042 | 0.0009 | 0.0229 | 0.009 | 0.0028 | 0.0004 | 0.0039 | 0 | 0.0044 | 0 | 0.0044 | 0 | 0.0015 | 0.0171 | 0 | 0.0039 |
Lipid Particles | 0.014 | 0.0011 | 0.0046 | 0.0109 | 0.0099 | 0.0062 | 0.0081 | 0.0016 | 0.0003 | 0 | 0.0043 | 0 | 0.0127 | 0.0008 | 0.0019 | 0.0599 | 0.0003 | 0.008 |
Mitochondria | 0.0034 | 0.0019 | 0.0052 | 0.0023 | 0.0843 | 0.0039 | 0.0033 | 0.0005 | 0.0036 | 0.0001 | 0.0071 | 0.0003 | 0.0038 | 0.0013 | 0.0099 | 0.0388 | 0.0005 | 0.0053 |
None | 0.0104 | 0.0001 | 0.0062 | 0.0417 | 0.0015 | 0.0017 | 0.001 | 0.0001 | 0 | 0 | 0.0018 | 0 | 0.0126 | 0 | 0.0004 | 0.0032 | 0 | 0.0001 |
Nuclear Periphery | 0.0103 | 0.0005 | 0.0014 | 0.0004 | 0.0029 | 0.0021 | 0.0107 | 0.0009 | 0.0065 | 0.0001 | 0.0575 | 0.0001 | 0.0171 | 0.0002 | 0.0119 | 0.0798 | 0.0005 | 0.0029 |
Nucleolus | 0.4663 | 0.5559 | 0.6096 | 0.6317 | 0.3167 | 0.5989 | 0.5195 | 0.5779 | 0.5598 | 0.5636 | 0.4472 | 0.5081 | 0.5373 | 0.6022 | 0.6437 | 0.2092 | 0.6283 | 0.5977 |
Nucleus | 0.3622 | 0.4235 | 0.3162 | 0.2635 | 0.3962 | 0.3239 | 0.3621 | 0.4088 | 0.4053 | 0.4347 | 0.3876 | 0.4894 | 0.3334 | 0.3865 | 0.2877 | 0.3724 | 0.3644 | 0.3571 |
Peroxisomes | 0.0145 | 0.0034 | 0.0086 | 0.0046 | 0.0152 | 0.0012 | 0.0029 | 0.0004 | 0 | 0 | 0.001 | 0 | 0.0082 | 0.0001 | 0.0108 | 0.0039 | 0 | 0.0001 |
Punctate Nuclear | 0.0187 | 0.0019 | 0.0019 | 0.0049 | 0.0009 | 0.0057 | 0.0328 | 0.0014 | 0.0002 | 0.0003 | 0.0064 | 0.0003 | 0.0128 | 0.0005 | 0.002 | 0.0183 | 0.0047 | 0.0112 |
Vacuole | 0.0038 | 0.0013 | 0.0006 | 0.0033 | 0.0008 | 0.0017 | 0.0037 | 0.0009 | 0.0018 | 0.0003 | 0.004 | 0.0003 | 0.0023 | 0.0048 | 0.0017 | 0.0104 | 0.0002 | 0.0011 |
Vacuole Periphery | 0.0016 | 0.0006 | 0.0003 | 0.0003 | 0.0015 | 0.0007 | 0.0023 | 0.0004 | 0.0025 | 0.0001 | 0.0075 | 0.0001 | 0.0021 | 0.0014 | 0.002 | 0.0139 | 0.0003 | 0.0025 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 46.7941 | 35.1381 | 29.7988 | 24.3834 | 32.1554 | 26.1902 | 21.9936 | 21.4099 | 16.0931 | 26.5698 |
Translational Efficiency | 2.0017 | 2.0354 | 2.2004 | 2.075 | 1.8313 | 1.8847 | 2.0754 | 1.3886 | 1.6417 | 1.9711 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
119 | 198 | 1964 | 1496 | 134 | 1757 | 82 | 1320 | 253 | 1955 | 2046 | 2816 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 954.42 | 1033.49 | 1140.62 | 1139.45 | 909.90 | 932.77 | 1240.58 | 1212.07 | 930.84 | 942.97 | 1144.63 | 1173.49 |
Standard Deviation | 142.55 | 157.54 | 170.01 | 223.32 | 146.38 | 144.37 | 174.98 | 207.67 | 146.29 | 148.89 | 171.33 | 219.14 |
Intensity Change Log 2 | — | 0.114828 | 0.257122 | 0.255641 | — | 0.035813 | 0.447235 | 0.413693 | — | 0.076805 | 0.353039 | 0.334944 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000420 | 0.000331 | 0.000529 | 0.000991 | 0.000388 | 0.000325 | 0.001034 | 0.000635 | 0.000403 | 0.000325 | 0.000549 | 0.000824 |
Bud Neck | 0.003389 | 0.005739 | 0.013887 | 0.029778 | 0.002322 | 0.005686 | 0.007011 | 0.025231 | 0.002824 | 0.005691 | 0.013611 | 0.027647 |
Bud Site | 0.000642 | 0.000405 | 0.000815 | 0.003223 | 0.000501 | 0.000734 | 0.001280 | 0.003758 | 0.000567 | 0.000700 | 0.000834 | 0.003474 |
Cell Periphery | 0.000079 | 0.000014 | 0.000021 | 0.000087 | 0.000081 | 0.000059 | 0.000035 | 0.000132 | 0.000080 | 0.000055 | 0.000021 | 0.000108 |
Cytoplasm | 0.002789 | 0.000041 | 0.000138 | 0.001430 | 0.000492 | 0.001768 | 0.002662 | 0.000550 | 0.001573 | 0.001593 | 0.000239 | 0.001018 |
Cytoplasmic Foci | 0.000055 | 0.000048 | 0.000322 | 0.000968 | 0.000196 | 0.001410 | 0.000828 | 0.001299 | 0.000130 | 0.001272 | 0.000343 | 0.001123 |
Eisosomes | 0.000041 | 0.000038 | 0.000040 | 0.000046 | 0.000049 | 0.000042 | 0.000075 | 0.000053 | 0.000045 | 0.000042 | 0.000042 | 0.000050 |
Endoplasmic Reticulum | 0.001892 | 0.001192 | 0.000927 | 0.001635 | 0.002202 | 0.001704 | 0.002939 | 0.001183 | 0.002056 | 0.001652 | 0.001007 | 0.001423 |
Endosome | 0.000238 | 0.000120 | 0.000149 | 0.002460 | 0.000226 | 0.001395 | 0.000628 | 0.001868 | 0.000231 | 0.001266 | 0.000168 | 0.002183 |
Golgi | 0.000185 | 0.000160 | 0.000427 | 0.001980 | 0.000412 | 0.001181 | 0.000450 | 0.001605 | 0.000306 | 0.001078 | 0.000428 | 0.001804 |
Lipid Particles | 0.000072 | 0.000045 | 0.000173 | 0.000200 | 0.000182 | 0.000421 | 0.001076 | 0.000104 | 0.000130 | 0.000383 | 0.000209 | 0.000155 |
Mitochondria | 0.000620 | 0.000571 | 0.001906 | 0.003278 | 0.000424 | 0.001973 | 0.000997 | 0.003380 | 0.000516 | 0.001831 | 0.001869 | 0.003326 |
Mitotic Spindle | 0.001879 | 0.000974 | 0.004677 | 0.013584 | 0.000244 | 0.006205 | 0.015149 | 0.007829 | 0.001013 | 0.005675 | 0.005097 | 0.010886 |
None | 0.008791 | 0.000458 | 0.000536 | 0.001481 | 0.003871 | 0.002276 | 0.001520 | 0.001063 | 0.006185 | 0.002092 | 0.000575 | 0.001285 |
Nuclear Periphery | 0.000167 | 0.000811 | 0.000431 | 0.002296 | 0.000072 | 0.000455 | 0.000329 | 0.001177 | 0.000117 | 0.000491 | 0.000427 | 0.001772 |
Nuclear Periphery Foci | 0.001314 | 0.002015 | 0.000298 | 0.002005 | 0.003424 | 0.001100 | 0.001136 | 0.000914 | 0.002431 | 0.001193 | 0.000332 | 0.001494 |
Nucleolus | 0.464929* | 0.432980* | 0.481021* | 0.409826* | 0.392907* | 0.401538* | 0.536856* | 0.468975* | 0.426783* | 0.404723* | 0.483259* | 0.437552* |
Nucleus | 0.510012* | 0.551692* | 0.491214* | 0.512726* | 0.588711* | 0.568166* | 0.412421* | 0.472588* | 0.551694* | 0.566497* | 0.488056* | 0.493912* |
Peroxisomes | 0.002055 | 0.001840 | 0.001695 | 0.001658 | 0.002850 | 0.001376 | 0.010921 | 0.001973 | 0.002476 | 0.001423 | 0.002064 | 0.001806 |
Vacuole | 0.000209 | 0.000254 | 0.000418 | 0.005684 | 0.000272 | 0.001220 | 0.002195 | 0.003158 | 0.000242 | 0.001122 | 0.000489 | 0.004500 |
Vacuole Periphery | 0.000221 | 0.000272 | 0.000378 | 0.004662 | 0.000175 | 0.000965 | 0.000460 | 0.002525 | 0.000196 | 0.000895 | 0.000381 | 0.003660 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.11 | -2.50 | -3.16 | -3.68 | -2.29 | 0.86 | -4.11 | -2.70 | -9.84 | 2.88 | 1.34 | -2.64 | -3.98 | -5.59 | -2.58 |
Bud Neck | -2.03 | -13.51 | -19.25 | -17.11 | -11.54 | -5.46 | -3.72 | -17.61 | -15.95 | -8.74 | -5.35 | -17.02 | -26.23 | -24.40 | -13.64 |
Bud Site | 0.88 | -2.05 | -6.22 | -7.41 | -5.86 | -1.42 | -2.88 | -5.40 | -5.12 | -2.31 | -0.96 | -4.05 | -8.17 | -8.04 | -6.99 |
Cell Periphery | 1.99 | 1.66 | -0.32 | -7.18 | -6.30 | 0.56 | 0.85 | -0.60 | -1.10 | -1.30 | 0.93 | 2.12 | -0.67 | -1.74 | -2.86 |
Cytoplasm | 1.08 | 1.03 | 0.44 | -5.71 | -5.19 | -2.02 | -0.98 | -0.77 | 1.77 | 0.95 | -0.20 | 0.85 | 0.28 | 0.92 | -1.91 |
Cytoplasmic Foci | 0.37 | -1.26 | -3.53 | -3.56 | -1.95 | -2.41 | -1.00 | -2.09 | 0.57 | -0.58 | -2.54 | -0.90 | -3.46 | 0.79 | -2.32 |
Eisosomes | 0.60 | 0.14 | -1.06 | -1.74 | -2.82 | 1.62 | -3.61 | -0.52 | -4.79 | 4.08 | 1.24 | 1.18 | -0.92 | -4.32 | -4.59 |
Endoplasmic Reticulum | 1.66 | 2.22 | 0.20 | -2.82 | -5.99 | 1.63 | -1.42 | 2.58 | 2.53 | 3.38 | 1.65 | 3.72 | 1.91 | 0.57 | -4.85 |
Endosome | 1.69 | 1.14 | -3.52 | -3.74 | -3.67 | -4.22 | -1.03 | -3.01 | -0.45 | -1.81 | -4.21 | 1.08 | -4.63 | -1.45 | -4.79 |
Golgi | 0.85 | -1.72 | -3.17 | -3.22 | -2.58 | -1.97 | -0.39 | -1.73 | -0.35 | -1.64 | -2.33 | -0.69 | -3.40 | -1.04 | -2.99 |
Lipid Particles | 1.03 | -0.94 | -2.65 | -3.03 | -0.51 | -1.17 | -1.00 | 1.42 | 1.62 | 1.12 | -1.44 | -0.52 | -0.72 | 1.27 | 0.22 |
Mitochondria | 0.36 | -3.12 | -12.46 | -12.79 | -3.37 | -3.85 | -4.46 | -5.14 | -2.16 | -3.71 | -3.62 | -3.47 | -9.88 | -3.71 | -3.15 |
Mitotic Spindle | 0.78 | -2.95 | -5.29 | -6.27 | -3.21 | -4.60 | -1.95 | -5.57 | -2.15 | 0.86 | -3.61 | -4.64 | -7.70 | -4.04 | -2.97 |
None | 1.76 | 1.72 | 1.51 | -6.36 | -5.56 | 0.85 | 1.16 | 1.41 | 2.29 | 0.37 | 1.67 | 2.27 | 1.96 | 1.60 | -5.85 |
Nuclear Periphery | -1.00 | -3.21 | -8.20 | -2.13 | -6.68 | -5.96 | -2.47 | -4.46 | -2.97 | -2.25 | -4.19 | -4.05 | -9.12 | -6.51 | -6.52 |
Nuclear Periphery Foci | -0.59 | 2.82 | -1.20 | 0.10 | -4.28 | 1.52 | 1.44 | 1.66 | 0.75 | 0.85 | 1.46 | 2.48 | 1.21 | -0.68 | -4.42 |
Nucleolus | 1.11 | -0.41 | 2.83 | 2.01 | 7.95 | -0.35 | -3.12 | -2.47 | -4.82 | 1.90 | 1.36 | -2.89 | 0.40 | -2.03 | 6.65 |
Nucleus | -1.48 | 0.83 | -0.25 | 1.93 | -2.60 | 0.90 | 4.65 | 4.76 | 9.07 | -1.94 | -0.90 | 3.75 | 3.14 | 8.60 | -1.36 |
Peroxisomes | 0.48 | 0.67 | 0.74 | 0.23 | 0.24 | 2.75 | -3.80 | 1.68 | -2.41 | 4.36 | 2.67 | 1.20 | 1.88 | -1.79 | 1.47 |
Vacuole | -0.80 | -3.57 | -6.21 | -6.16 | -5.83 | -3.28 | -1.90 | -6.70 | -3.89 | 0.13 | -3.41 | -3.69 | -8.36 | -6.01 | -7.44 |
Vacuole Periphery | -1.00 | -5.10 | -5.90 | -5.82 | -5.63 | -3.28 | -6.84 | -5.99 | -3.20 | -5.09 | -3.24 | -8.44 | -7.93 | -5.38 | -7.40 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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