Standard name
Human Ortholog
Description Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0.1 0.06 0 0.05 0 0 0 0
Bud 0 0 0 0 0 0.06 0.05 0.06 0.05 0.06 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.58 0.67 0.43 0.49 0.48 0.48 0.4 0.43 0.45 0.5 0.4 0.42 0.79 0.7 0.67 0.06 0.1 0.1 0.23 0.21 0.16 0.25 0.25 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0
Endosome 0.14 0.09 0 0.06 0.1 0 0 0 0 0 0 0 0.06 0.15 0.18 0.19 0.17 0.19 0.23 0.19 0.23 0.13 0.13 0.11
Golgi 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.58 0.6 0.52 0.08 0.09 0.17 0.06 0.07 0.05
Mitochondria 0.25 0.15 0.53 0.54 0.52 0.63 0.7 0.65 0.68 0.61 0.74 0.69 0 0 0 0.11 0.1 0.12 0.27 0.28 0.27 0.37 0.32 0.31
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0 0 0
SpindlePole 0 0 0 0 0.05 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0.1 0.07 0.13 0 0.09 0 0 0.09 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 8 1 0 0 1 0 0 0 0 0 0 6 2 0 6 9 3 9 16 8 3 4 8
Bud 2 4 8 8 17 29 31 39 28 36 23 43 2 1 2 1 3 0 3 7 2 0 4 4
Bud Neck 0 0 0 0 1 1 1 2 2 2 1 1 3 2 1 0 0 0 0 2 1 1 2 1
Bud Site 0 0 1 1 0 7 4 7 5 3 9 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 2 0 0 0 1 1 1 2 2 5 4 0 0 0 0 0 1 0 1 0 0 0 0
Cytoplasm 108 181 101 120 193 226 243 276 235 291 194 292 100 151 168 5 9 5 52 73 42 33 52 70
Endoplasmic Reticulum 2 7 1 3 0 2 1 0 0 0 3 2 5 2 3 3 8 1 1 4 1 2 4 3
Endosome 26 24 10 15 38 3 6 8 3 4 2 2 8 33 44 15 15 10 52 68 60 17 27 25
Golgi 5 13 3 2 0 0 0 0 0 0 0 0 4 4 7 47 52 27 19 30 44 7 14 12
Mitochondria 46 40 124 134 209 298 423 416 356 354 361 475 3 7 8 9 9 6 61 98 71 49 65 72
Nucleus 0 0 0 2 0 1 3 4 2 3 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 2 1 1 0 0 1 0 0 0 0 0 0 1 0 0 0 1 0 0
Nucleolus 0 0 1 0 2 0 3 4 4 1 4 3 0 1 0 0 1 0 0 0 0 0 0 0
Peroxisomes 1 1 1 6 14 3 8 8 5 6 3 4 0 8 13 0 1 1 9 6 14 3 3 1
SpindlePole 3 1 9 9 21 10 15 28 19 26 27 24 2 9 5 1 1 2 11 8 8 2 3 5
Vac/Vac Membrane 5 17 2 7 10 8 11 6 6 6 6 5 3 17 22 8 6 7 7 30 4 5 18 22
Unique Cell Count 185 272 235 247 400 475 604 643 520 583 489 690 126 217 250 81 87 52 232 353 267 132 206 235
Labelled Cell Count 201 299 262 307 505 592 751 800 667 734 639 865 136 237 273 95 114 65 232 353 267 132 206 235


Early Golgi, Late Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.9 4.1 4.0 3.7 3.7 3.2 3.4 3.1 3.0 3.0 2.9 5.9 5.5 5.8 9.5 9.6 9.9 5.6 6.0 6.2
Std Deviation (1e-4) 0.9 1.0 1.1 1.5 1.3 1.4 1.1 1.5 1.1 1.2 1.2 0.7 1.5 1.5 1.6 2.0 2.0 2.0 1.6 1.9 1.9
Intensity Change (Log2) – – – -0.03 -0.13 -0.16 -0.37 -0.27 -0.42 -0.45 -0.45 -0.5 0.54 0.43 0.5 1.21 1.23 1.27 0.47 0.56 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5582 2.7338 3.0273 2.304 2.4598 2.7826 2.9426 3.6885 3.1903 3.5154 2.6363 3.5345 1.1874 2.3637 1.7175 2.2673 1.7499 2.1079
Actin 0.0184 0.0099 0.024 0.0017 0.0173 0.0119 0.0376 0.004 0.0143 0.0059 0.0012 0.0194 0.0076 0.0029 0.007 0.0004 0.0061 0.0044
Bud 0.0004 0.0034 0.0007 0.0009 0.0008 0.0013 0.0005 0.0003 0.0005 0.0002 0.0002 0.0007 0.0004 0.0002 0.001 0.0001 0.0004 0.0001
Bud Neck 0.0008 0.0005 0.0047 0.0004 0.0006 0.001 0.0028 0.0005 0.0005 0.0005 0.0011 0.0013 0.0003 0.0003 0.0005 0.0002 0.0003 0.0005
Bud Periphery 0.0004 0.0051 0.0012 0.0013 0.001 0.0024 0.0006 0.0002 0.0007 0.0001 0.0002 0.0013 0.0004 0.0001 0.0027 0.0001 0.0017 0.0001
Bud Site 0.001 0.0069 0.0029 0.0006 0.0083 0.0004 0.0043 0.0029 0.0012 0.0003 0.0005 0.0004 0.0009 0.0011 0.0007 0.0001 0.0012 0.0002
Cell Periphery 0.0001 0.0002 0.0001 0.0001 0.0003 0.0001 0.0002 0.0001 0.0003 0 0.0001 0.0001 0.0002 0 0.0001 0 0.0002 0
Cytoplasm 0.0142 0.0184 0.0085 0.0074 0.0046 0.0077 0.0128 0.0153 0.0275 0.0026 0.0295 0.0241 0.0084 0.0176 0.0109 0.006 0.0043 0.006
Cytoplasmic Foci 0.167 0.1929 0.161 0.1767 0.168 0.1427 0.1974 0.2183 0.1654 0.1836 0.1768 0.1491 0.1459 0.1655 0.1421 0.0991 0.0835 0.1425
Eisosomes 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0004 0.0001 0.0004 0 0 0.0001 0.0001 0 0.0001 0 0.0004 0
Endoplasmic Reticulum 0.0027 0.0009 0.001 0.0034 0.0035 0.0008 0.0011 0.0006 0.0007 0.0003 0.0017 0.0013 0.0032 0.0011 0.0017 0.0005 0.0007 0.0006
Endosome 0.3285 0.3121 0.3002 0.3946 0.3966 0.265 0.2981 0.3469 0.3128 0.3529 0.3462 0.2851 0.3721 0.3773 0.353 0.4554 0.4213 0.2777
Golgi 0.2997 0.3107 0.354 0.264 0.2879 0.4269 0.2491 0.3188 0.3277 0.3365 0.2417 0.3336 0.2814 0.3415 0.3461 0.3974 0.3445 0.4795
Lipid Particles 0.0623 0.0232 0.0216 0.0121 0.0313 0.0215 0.0597 0.0297 0.0065 0.0123 0.0433 0.0288 0.0538 0.0082 0.0184 0.0067 0.0089 0.0239
Mitochondria 0.0291 0.0306 0.0377 0.0531 0.0269 0.0424 0.0146 0.0103 0.0677 0.011 0.013 0.0293 0.0463 0.0228 0.0373 0.0125 0.0529 0.0217
None 0.0096 0.0292 0.0198 0.0091 0.0011 0.0136 0.0351 0.0087 0.0074 0.0001 0.086 0.0707 0.0196 0.0328 0.0335 0.0003 0.0315 0.0066
Nuclear Periphery 0.0075 0.0012 0.0019 0.0108 0.0091 0.0017 0.0006 0.0002 0.0002 0.0001 0.0008 0.0009 0.0009 0.0002 0.0007 0.0001 0.0014 0.0001
Nucleolus 0.0002 0.0002 0.0005 0.0012 0.0011 0.0002 0.0005 0.0001 0.0001 0 0.0004 0.0002 0.0002 0.0001 0.0002 0 0.0016 0
Nucleus 0.0017 0.0004 0.0017 0.004 0.003 0.0003 0.0003 0.0001 0.0002 0 0.0005 0.0007 0.0002 0.0002 0.0002 0 0.0015 0
Peroxisomes 0.0482 0.0476 0.05 0.044 0.0261 0.0533 0.0725 0.0361 0.0601 0.0914 0.0445 0.0455 0.0506 0.022 0.0379 0.0157 0.0048 0.0326
Punctate Nuclear 0.0019 0.0009 0.0014 0.0068 0.0017 0.0023 0.004 0.0004 0.0002 0.0003 0.0006 0.0012 0.0007 0.0004 0.0008 0.0001 0.0046 0.0002
Vacuole 0.0036 0.0036 0.0045 0.0037 0.0073 0.0022 0.0061 0.005 0.0031 0.001 0.0078 0.0036 0.0041 0.0033 0.0029 0.0022 0.021 0.0014
Vacuole Periphery 0.0027 0.002 0.0025 0.004 0.0033 0.0021 0.0017 0.0015 0.0024 0.001 0.0038 0.0025 0.0028 0.0021 0.0022 0.003 0.0074 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.4582 21.3715 31.7171 43.9481 35.7653 14.7039 35.3219 32.5344 36.6007 46.7344
Translational Efficiency 1.0668 1.6688 1.1467 0.8652 1.3516 2.9851 1.3137 1.412 1.3964 1.1835

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
967 1624 1338 918 1762 1333 2515 1517 2729 2957 3853 2435

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 615.53 770.49 1070.07 943.25 731.33 744.18 1026.62 910.45 690.30 758.63 1041.71 922.82
Standard Deviation 85.57 111.11 174.81 143.73 93.03 103.32 153.17 141.66 106.07 108.46 162.34 143.33
Intensity Change Log 2 — 0.323947 0.797804 0.615811 — 0.025129 0.489308 0.316057 — 0.169404 0.638514 0.460808

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000952 0.001751 0.004401 0.011629 0.000752 0.000848 0.008502 0.013995 0.000823 0.001344 0.007078 0.013103
Bud Neck 0.014332 0.013452 0.001084 0.006765 0.008977 0.019693 0.001871 0.009509 0.010874 0.016265 0.001598 0.008474
Bud Site 0.013580 0.021109 0.011475 0.040310 0.009360 0.018707 0.015362 0.043363 0.010855 0.020026 0.014012 0.042212
Cell Periphery 0.000562 0.000125 0.000077 0.000085 0.000098 0.000179 0.000065 0.000096 0.000263 0.000149 0.000069 0.000092
Cytoplasm 0.119675* 0.159337* 0.021272 0.102134* 0.138425* 0.134349* 0.028552 0.116944* 0.131781* 0.148073* 0.026024 0.111361*
Cytoplasmic Foci 0.285969* 0.386187* 0.223374* 0.272099* 0.392830* 0.339939* 0.269000* 0.284940* 0.354964* 0.365339* 0.253156* 0.280099*
Eisosomes 0.000502 0.000152 0.000034 0.000064 0.000176 0.000173 0.000051 0.000099 0.000292 0.000161 0.000046 0.000086
Endoplasmic Reticulum 0.000909 0.000766 0.002113 0.001468 0.000873 0.001071 0.000582 0.000682 0.000886 0.000904 0.001113 0.000978
Endosome 0.066999 0.099685 0.160245 0.145155 0.091959 0.141839 0.198865 0.149299 0.083115 0.118688 0.185453 0.147737
Golgi 0.233319* 0.143061* 0.438342* 0.094867 0.196144* 0.114783* 0.314477* 0.120977* 0.209317* 0.130313* 0.357491* 0.111133*
Lipid Particles 0.033058 0.013541 0.004519 0.005969 0.017710 0.019415 0.004297 0.007701 0.023148 0.016189 0.004374 0.007048
Mitochondria 0.157121* 0.039559 0.011436 0.003275 0.061858 0.069455 0.018856 0.011071 0.095613 0.053036 0.016279 0.008132
Mitotic Spindle 0.004219 0.023586 0.052915 0.124816* 0.013405 0.015905 0.076961 0.057020 0.010150 0.020124 0.068611 0.082580
None 0.003482 0.002215 0.000107 0.000352 0.001271 0.002386 0.000212 0.000291 0.002055 0.002292 0.000176 0.000314
Nuclear Periphery 0.000151 0.000212 0.000514 0.000545 0.000159 0.000605 0.000177 0.000200 0.000156 0.000389 0.000294 0.000330
Nuclear Periphery Foci 0.000241 0.000574 0.002747 0.001827 0.000473 0.000454 0.000535 0.000938 0.000391 0.000520 0.001303 0.001274
Nucleolus 0.000920 0.001383 0.000089 0.000475 0.000501 0.001595 0.000131 0.000442 0.000650 0.001479 0.000116 0.000455
Nucleus 0.014861 0.018043 0.001981 0.019820 0.011094 0.021708 0.003349 0.010432 0.012429 0.019695 0.002874 0.013971
Peroxisomes 0.032542 0.030660 0.005935 0.066751 0.020872 0.026600 0.020402 0.095096 0.025007 0.028830 0.015378 0.084410
Vacuole 0.008892 0.038024 0.042524 0.098967 0.025947 0.058723 0.031696 0.074955 0.019904 0.047355 0.035456 0.084007
Vacuole Periphery 0.007714 0.006578 0.014815 0.002627 0.007116 0.011573 0.006056 0.001949 0.007328 0.008829 0.009098 0.002205

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.62 -4.30 -5.80 -5.03 -2.93 -0.42 -8.25 -8.44 -8.32 -2.35 -1.82 -9.28 -10.24 -9.45 -3.40
Bud Neck 0.50 9.27 3.34 3.49 -9.34 -6.51 8.68 -1.32 5.19 -8.62 -4.81 12.65 0.94 5.73 -11.85
Bud Site -2.87 0.69 -6.86 -4.97 -8.12 -4.67 -5.72 -12.36 -7.20 -9.22 -6.10 -3.47 -14.02 -8.74 -12.15
Cell Periphery 7.96 8.89 8.75 3.79 -1.04 -3.67 4.87 0.49 3.81 -3.79 5.01 9.53 8.39 4.99 -3.87
Cytoplasm -4.69 13.71 -1.72 2.58 -14.67 0.56 18.80 0.25 -0.29 -16.71 -2.91 23.22 -0.77 1.96 -22.10
Cytoplasmic Foci -11.32 7.84 6.89 19.32 0.07 6.67 20.18 17.26 9.44 0.03 -1.73 20.46 17.97 20.21 0.33
Eisosomes 4.58 6.15 5.71 9.26 -7.92 0.19 10.28 5.89 6.63 -7.90 4.51 8.70 7.19 10.11 -10.19
Endoplasmic Reticulum 0.54 -2.29 -3.56 -6.07 0.47 -1.04 1.60 -0.00 1.18 -2.13 -0.15 -1.48 -2.82 -3.46 -0.37
Endosome -6.99 -18.34 -7.43 -2.09 7.53 -9.02 -23.19 -7.61 1.49 11.44 -9.94 -29.24 -10.31 -1.38 13.85
Golgi 9.03 -16.88 16.72 10.78 39.47* 10.66 -14.38 12.72 1.59 29.77* 13.17 -21.48 20.35 8.10 46.83*
Lipid Particles 7.66 11.68 11.09 7.35 -2.32 -0.88 10.52 6.80 7.02 -3.60 4.91 15.61 12.49 9.20 -4.25
Mitochondria 15.57 19.76 21.30 16.07 5.74 -1.52 13.09 16.09 15.18 5.20 10.85 22.82 25.87 20.56 7.43
Mitotic Spindle -8.76 -11.91 -14.94 -11.92 -7.17 -1.16 -16.73 -10.85 -9.92 4.26 -5.94 -20.44 -18.08 -14.91 -1.85
None 1.33 4.01 3.45 4.56 -5.95 -1.46 4.87 4.75 2.77 -1.00 -0.52 5.68 5.01 4.85 -3.19
Nuclear Periphery -1.85 -4.47 -6.39 -6.03 -3.60 -3.52 -2.07 -5.90 2.06 -3.97 -3.91 -4.90 -7.74 -3.32 -4.68
Nuclear Periphery Foci -3.13 -5.64 -7.75 -6.54 -0.19 0.16 -1.11 -2.07 -2.19 -1.67 -1.47 -5.57 -6.36 -5.71 -1.43
Nucleolus -1.56 4.36 -0.46 1.04 -4.00 -5.04 5.62 -0.68 4.17 -3.68 -4.46 6.66 -0.85 3.49 -5.41
Nucleus -1.49 6.83 -5.91 -5.20 -10.09 -4.98 5.61 -3.67 1.54 -7.67 -5.04 8.64 -6.95 -2.76 -12.51
Peroxisomes 0.70 11.87 -4.71 -5.20 -11.22 -2.57 0.06 -15.30 -13.32 -15.24 -2.25 6.29 -15.39 -13.86 -18.56
Vacuole -14.70 -13.23 -21.24 -17.10 -15.75 -10.33 -4.95 -16.92 -9.53 -14.30 -14.55 -11.38 -26.46 -19.33 -21.09
Vacuole Periphery 1.47 -6.10 7.29 8.39 12.88 -4.82 1.85 10.00 12.58 10.77 -2.53 -2.93 12.30 15.66 16.46
Standard name
Human Ortholog
Description Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
Localization
Cell Percentages mitochondrion (17%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Tvp23

Tvp23


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tvp23-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available