Standard name | |
---|---|
Human Ortholog | |
Description | Essential evolutionarily conserved nucleolar protein; necessary for biogenesis of 60S ribosomal subunits and for processing of pre-rRNAs to mature rRNA; associated with several distinct 66S pre-ribosomal particles |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 6 | 4 | 7 | 10 | 8 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 2 | 2 | 2 | 6 | 6 |
Bud Neck | 0 | 0 | 0 | 17 | 2 | 1 | 3 | 4 | 3 | 1 | 5 | 1 | 4 | 11 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 4 | 0 | 5 | 2 | 3 | 25 | 17 | 10 | 27 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 11 |
Cytoplasm | 6 | 4 | 7 | 5 | 2 | 9 | 5 | 13 | 8 | 10 | 5 | 5 | 9 | 26 | 46 | 1 | 5 | 4 | 0 | 0 | 0 | 33 | 51 | 52 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 5 |
Endosome | 2 | 1 | 10 | 8 | 44 | 91 | 93 | 121 | 36 | 57 | 32 | 53 | 4 | 11 | 20 | 2 | 2 | 0 | 1 | 1 | 3 | 1 | 12 | 25 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 6 |
Mitochondria | 0 | 8 | 4 | 5 | 4 | 25 | 18 | 32 | 167 | 93 | 128 | 87 | 6 | 7 | 1 | 17 | 10 | 12 | 1 | 1 | 7 | 5 | 15 | 29 |
Nucleus | 72 | 89 | 98 | 12 | 59 | 68 | 78 | 104 | 128 | 118 | 142 | 167 | 22 | 24 | 25 | 35 | 37 | 43 | 39 | 68 | 56 | 22 | 44 | 20 |
Nuclear Periphery | 9 | 14 | 24 | 1 | 10 | 9 | 11 | 20 | 27 | 20 | 19 | 23 | 1 | 2 | 1 | 1 | 1 | 0 | 8 | 5 | 26 | 6 | 12 | 19 |
Nucleolus | 69 | 68 | 211 | 310 | 291 | 402 | 536 | 620 | 414 | 496 | 326 | 401 | 142 | 257 | 279 | 109 | 87 | 87 | 108 | 115 | 277 | 79 | 173 | 132 |
Peroxisomes | 0 | 1 | 0 | 17 | 1 | 18 | 10 | 13 | 2 | 7 | 0 | 10 | 3 | 6 | 9 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
SpindlePole | 0 | 0 | 18 | 140 | 86 | 181 | 206 | 260 | 86 | 172 | 71 | 144 | 32 | 88 | 73 | 3 | 0 | 2 | 3 | 1 | 12 | 0 | 24 | 29 |
Vac/Vac Membrane | 7 | 7 | 17 | 0 | 6 | 8 | 23 | 32 | 15 | 18 | 17 | 17 | 1 | 2 | 4 | 4 | 4 | 7 | 8 | 17 | 17 | 13 | 33 | 36 |
Unique Cell Count | 150 | 169 | 329 | 341 | 369 | 533 | 711 | 841 | 633 | 721 | 550 | 645 | 174 | 309 | 359 | 143 | 124 | 133 | 178 | 215 | 406 | 178 | 397 | 391 |
Labelled Cell Count | 165 | 192 | 389 | 519 | 506 | 817 | 990 | 1228 | 915 | 1016 | 765 | 943 | 224 | 435 | 465 | 173 | 146 | 156 | 178 | 215 | 406 | 178 | 397 | 391 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.4 | 11.4 | 13.6 | 12.2 | 11.1 | 8.7 | 8.2 | 8.2 | 6.4 | 7.0 | 6.6 | 6.9 | 13.1 | 13.1 | 12.3 | 17.4 | 19.4 | 22.9 | 21.9 | 19.3 | 20.3 |
Std Deviation (1e-4) | 3.6 | 3.1 | 3.3 | 4.0 | 2.7 | 2.1 | 1.7 | 2.0 | 1.8 | 1.7 | 1.9 | 1.8 | 6.6 | 6.9 | 6.6 | 5.6 | 7.1 | 8.7 | 12.0 | 10.8 | 12.1 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.29 | -0.65 | -0.73 | -0.73 | -1.09 | -0.96 | -1.04 | -0.99 | -0.06 | -0.05 | -0.15 | 0.36 | 0.52 | 0.75 | 0.69 | 0.5 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 3.7 | 2.8 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | 0 | -0.5 | 0 | -0.7 | -1.0 | -0.9 | 0 | 0 | 1.9 | 3.6 | 5.3 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -0.6 | 4.4 | 6.2 | 5.0 | 5.5 | 1.8 | 3.0 | 1.9 | 3.1 | 0 | 0.4 | 1.6 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.7 | 1.4 | 2.3 | 9.7 | 6.1 | 8.9 | 6.2 | 0 | 1.0 | 0 | 5.2 | 0 | 0 |
Nucleus | -9.2 | -4.4 | -6.2 | -7.5 | -7.1 | -3.3 | -5.0 | -1.3 | -1.3 | -4.3 | -7.1 | -7.8 | -1.2 | 0 | 0.5 |
Nuclear Periphery | -4.8 | -2.8 | -4.2 | -4.8 | -4.0 | -2.0 | -3.4 | -2.6 | -2.6 | -3.3 | -4.2 | -4.9 | -2.9 | -2.7 | -3.2 |
Nucleolus | 8.3 | 4.3 | 3.6 | 3.8 | 3.3 | 0.4 | 1.5 | -1.4 | -0.6 | 4.1 | 5.4 | 3.9 | 2.6 | 1.2 | 0.3 |
Peroxisomes | 4.1 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 10.8 | 6.6 | 9.6 | 8.6 | 9.2 | 3.8 | 7.2 | 3.5 | 6.7 | 4.6 | 7.8 | 5.7 | -1.6 | 0 | -1.9 |
Vacuole | -4.3 | -2.6 | -3.1 | -1.5 | -1.0 | -2.3 | -2.2 | -1.5 | -2.0 | -2.6 | -3.4 | -3.1 | -1.1 | -0.9 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.5431 | 10.5264 | 9.5033 | 8.8931 | 9.7245 | 9.6572 | 10.9297 | 12.6156 | 11.8052 | 10.1854 | 10.8607 | 11.7281 | 9.3332 | 10.8086 | 10.536 | 10.542 | 9.2078 | 10.0475 |
Actin | 0.0113 | 0.0001 | 0.0081 | 0.0004 | 0.0001 | 0.0002 | 0.0188 | 0.0002 | 0.0003 | 0.0002 | 0.0008 | 0.0001 | 0.0117 | 0.0051 | 0.0116 | 0.0001 | 0.0203 | 0.0002 |
Bud | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 |
Bud Neck | 0.0006 | 0.0007 | 0.0007 | 0.0004 | 0.0006 | 0.0006 | 0.0005 | 0.0003 | 0.0002 | 0.0008 | 0.0009 | 0.0003 | 0.0016 | 0.0005 | 0.0002 | 0.0004 | 0.0008 | 0.0005 |
Bud Periphery | 0.001 | 0.0005 | 0.0003 | 0.0003 | 0.0015 | 0.0005 | 0.0006 | 0.0004 | 0.0002 | 0.0005 | 0.0007 | 0.0003 | 0.0006 | 0.0006 | 0.0002 | 0.0004 | 0.0006 | 0.0004 |
Bud Site | 0.0013 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0016 | 0.0002 | 0.0011 | 0.0001 | 0.0011 | 0.0001 | 0.0024 | 0.0009 | 0.0012 | 0.0002 | 0.0015 | 0.0001 |
Cell Periphery | 0.0007 | 0.0011 | 0.0003 | 0.0002 | 0.0012 | 0.0008 | 0.0006 | 0.0004 | 0.0003 | 0.0007 | 0.0007 | 0.0005 | 0.0011 | 0.0008 | 0.0002 | 0.0002 | 0.0003 | 0.0004 |
Cytoplasm | 0.0009 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0.0013 | 0 | 0.0016 | 0.0001 | 0.0004 | 0 | 0.0018 | 0.0001 | 0.0001 | 0 | 0.0005 | 0 |
Cytoplasmic Foci | 0.0072 | 0.0005 | 0.009 | 0.0004 | 0.0004 | 0.001 | 0.0181 | 0.0007 | 0.0067 | 0.0024 | 0.0135 | 0.0004 | 0.0088 | 0.0038 | 0.0014 | 0.0036 | 0.0056 | 0.0005 |
Eisosomes | 0.0011 | 0 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0015 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0018 | 0.0001 | 0.0003 | 0.0006 | 0.0004 | 0.0001 | 0.0011 | 0 | 0.0009 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0009 | 0.0001 |
Endosome | 0.0095 | 0.0007 | 0.002 | 0.001 | 0.0002 | 0.0003 | 0.0089 | 0.0002 | 0.0051 | 0.0008 | 0.0025 | 0.0001 | 0.0033 | 0.0049 | 0.0003 | 0.0003 | 0.0154 | 0.0002 |
Golgi | 0.004 | 0.0003 | 0.0019 | 0.0002 | 0.0002 | 0.0005 | 0.004 | 0.0002 | 0.0011 | 0.0009 | 0.0014 | 0.0002 | 0.0008 | 0.0024 | 0.0006 | 0.0006 | 0.0232 | 0.0003 |
Lipid Particles | 0.0319 | 0.0058 | 0.0367 | 0.0033 | 0.0134 | 0.024 | 0.04 | 0.0078 | 0.0191 | 0.0521 | 0.0355 | 0.0116 | 0.0107 | 0.0112 | 0.004 | 0.0528 | 0.0074 | 0.0223 |
Mitochondria | 0.0183 | 0.001 | 0.005 | 0.0006 | 0.0044 | 0.0082 | 0.0099 | 0.0043 | 0.001 | 0.001 | 0.0275 | 0.0023 | 0.0097 | 0.005 | 0.0019 | 0.0019 | 0.0087 | 0.0085 |
None | 0.0053 | 0.0001 | 0.0025 | 0.0002 | 0.0002 | 0.0002 | 0.0058 | 0.0001 | 0.0015 | 0.0002 | 0.0012 | 0.0001 | 0.0035 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0001 |
Nuclear Periphery | 0.0981 | 0.0472 | 0.0734 | 0.2706 | 0.1477 | 0.1627 | 0.0829 | 0.0554 | 0.0628 | 0.2811 | 0.1919 | 0.1461 | 0.0256 | 0.0384 | 0.0019 | 0.0329 | 0.2281 | 0.1771 |
Nucleolus | 0.6505 | 0.7964 | 0.7361 | 0.5404 | 0.6847 | 0.6547 | 0.6056 | 0.7586 | 0.8035 | 0.4861 | 0.4923 | 0.7141 | 0.766 | 0.7277 | 0.8508 | 0.7764 | 0.4337 | 0.6154 |
Nucleus | 0.1139 | 0.1191 | 0.0955 | 0.1464 | 0.1209 | 0.1096 | 0.1042 | 0.1578 | 0.0689 | 0.1408 | 0.1577 | 0.1008 | 0.1146 | 0.1694 | 0.1183 | 0.1165 | 0.219 | 0.1477 |
Peroxisomes | 0.0058 | 0.0001 | 0.0077 | 0.0001 | 0.0006 | 0.0002 | 0.0065 | 0.0002 | 0.0023 | 0.0004 | 0.0019 | 0.0001 | 0.0007 | 0.007 | 0.0035 | 0.0025 | 0.0023 | 0.0002 |
Punctate Nuclear | 0.0184 | 0.0169 | 0.0141 | 0.0212 | 0.0098 | 0.014 | 0.0557 | 0.0058 | 0.0177 | 0.0099 | 0.0351 | 0.0068 | 0.0188 | 0.0038 | 0.0014 | 0.0072 | 0.015 | 0.0076 |
Vacuole | 0.0042 | 0.0041 | 0.0014 | 0.0009 | 0.0032 | 0.0026 | 0.0027 | 0.0011 | 0.0015 | 0.0037 | 0.0049 | 0.0013 | 0.0049 | 0.0096 | 0.0005 | 0.0008 | 0.0013 | 0.0016 |
Vacuole Periphery | 0.0138 | 0.0048 | 0.0037 | 0.0122 | 0.0102 | 0.0196 | 0.0304 | 0.006 | 0.0041 | 0.0177 | 0.0296 | 0.0145 | 0.0111 | 0.0081 | 0.0013 | 0.0028 | 0.0148 | 0.0165 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 44.8217 | 40.0678 | 41.1002 | 35.1756 | 52.131 | 46.1144 | 37.8316 | 30.9622 | 24.9777 | 48.2196 |
Translational Efficiency | 2.9381 | 3.1781 | 2.2344 | 2.1049 | 2.2138 | 1.6984 | 2.0526 | 2.0586 | 2.0101 | 1.9455 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1051 | 735 | 208 | 1512 | 1970 | 1954 | 2419 | 1651 | 3021 | 2689 | 2627 | 3163 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 962.27 | 1183.84 | 1653.44 | 1770.06 | 1002.69 | 1302.17 | 1617.43 | 1955.44 | 988.63 | 1269.83 | 1620.28 | 1866.82 |
Standard Deviation | 193.78 | 283.83 | 278.24 | 513.68 | 211.65 | 256.85 | 319.91 | 488.95 | 206.51 | 269.70 | 316.96 | 509.41 |
Intensity Change Log 2 | — | 0.298960 | 0.780957 | 0.879285 | — | 0.377042 | 0.689828 | 0.963618 | — | 0.339332 | 0.735174 | 0.922934 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000923 | 0.000933 | 0.000769 | 0.001518 | 0.000494 | 0.000558 | 0.001057 | 0.001240 | 0.000643 | 0.000661 | 0.001034 | 0.001373 |
Bud Neck | 0.001360 | 0.002241 | 0.003068 | 0.010582 | 0.001591 | 0.001862 | 0.003373 | 0.006552 | 0.001510 | 0.001965 | 0.003349 | 0.008478 |
Bud Site | 0.000316 | 0.000554 | 0.000592 | 0.002823 | 0.000279 | 0.000284 | 0.000204 | 0.000942 | 0.000292 | 0.000358 | 0.000234 | 0.001841 |
Cell Periphery | 0.000067 | 0.000088 | 0.000018 | 0.000342 | 0.000051 | 0.000040 | 0.000039 | 0.000100 | 0.000057 | 0.000053 | 0.000038 | 0.000216 |
Cytoplasm | 0.000667 | 0.000512 | 0.000028 | 0.002864 | 0.000666 | 0.000133 | 0.000083 | 0.000548 | 0.000667 | 0.000237 | 0.000079 | 0.001655 |
Cytoplasmic Foci | 0.000422 | 0.000988 | 0.000667 | 0.004055 | 0.000447 | 0.000803 | 0.000154 | 0.003478 | 0.000439 | 0.000854 | 0.000194 | 0.003754 |
Eisosomes | 0.000051 | 0.000078 | 0.000061 | 0.000092 | 0.000077 | 0.000084 | 0.000099 | 0.000114 | 0.000068 | 0.000083 | 0.000096 | 0.000103 |
Endoplasmic Reticulum | 0.002608 | 0.001459 | 0.000660 | 0.000947 | 0.001551 | 0.000916 | 0.000694 | 0.000337 | 0.001919 | 0.001064 | 0.000691 | 0.000629 |
Endosome | 0.000310 | 0.000347 | 0.000113 | 0.001582 | 0.000821 | 0.000588 | 0.000059 | 0.000728 | 0.000643 | 0.000522 | 0.000063 | 0.001136 |
Golgi | 0.000377 | 0.000695 | 0.000368 | 0.001708 | 0.000267 | 0.000644 | 0.000075 | 0.001085 | 0.000305 | 0.000658 | 0.000098 | 0.001383 |
Lipid Particles | 0.000994 | 0.000255 | 0.000090 | 0.000372 | 0.000137 | 0.000312 | 0.000013 | 0.000287 | 0.000435 | 0.000297 | 0.000019 | 0.000327 |
Mitochondria | 0.002035 | 0.003959 | 0.002618 | 0.008126 | 0.001526 | 0.002490 | 0.002250 | 0.008409 | 0.001703 | 0.002892 | 0.002279 | 0.008274 |
Mitotic Spindle | 0.001344 | 0.002604 | 0.015911 | 0.012910 | 0.001301 | 0.002656 | 0.000910 | 0.006083 | 0.001316 | 0.002642 | 0.002098 | 0.009346 |
None | 0.002390 | 0.002619 | 0.000266 | 0.004140 | 0.001233 | 0.000968 | 0.000412 | 0.001175 | 0.001635 | 0.001419 | 0.000401 | 0.002592 |
Nuclear Periphery | 0.010812 | 0.008229 | 0.048180 | 0.042133 | 0.011144 | 0.007771 | 0.021263 | 0.038937 | 0.011028 | 0.007897 | 0.023394 | 0.040465 |
Nuclear Periphery Foci | 0.006226 | 0.007530 | 0.017082 | 0.010907 | 0.010079 | 0.005305 | 0.003510 | 0.015130 | 0.008738 | 0.005913 | 0.004584 | 0.013111 |
Nucleolus | 0.513559* | 0.586610* | 0.747131* | 0.532871* | 0.601471* | 0.609805* | 0.529404* | 0.645166* | 0.570887* | 0.603465* | 0.546643* | 0.591486* |
Nucleus | 0.444971* | 0.365678* | 0.133300* | 0.315261* | 0.357945* | 0.353293* | 0.420621* | 0.204752* | 0.388221* | 0.356679* | 0.397872* | 0.257578* |
Peroxisomes | 0.004137 | 0.006435 | 0.023782 | 0.010614 | 0.004678 | 0.005276 | 0.003546 | 0.013435 | 0.004489 | 0.005593 | 0.005149 | 0.012087 |
Vacuole | 0.001863 | 0.002528 | 0.001377 | 0.006981 | 0.001340 | 0.001757 | 0.001195 | 0.007268 | 0.001522 | 0.001968 | 0.001210 | 0.007131 |
Vacuole Periphery | 0.004568 | 0.005658 | 0.003918 | 0.029172 | 0.002903 | 0.004454 | 0.011039 | 0.044237 | 0.003483 | 0.004783 | 0.010475 | 0.037035 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.07 | 0.46 | -5.30 | -5.46 | -3.60 | -2.36 | -15.78 | -14.69 | -13.32 | -3.19 | -0.34 | -7.98 | -13.91 | -15.02 | -7.05 |
Bud Neck | -3.72 | -3.48 | -13.56 | -12.14 | -9.40 | -1.18 | -7.46 | -12.69 | -11.47 | -7.81 | -2.62 | -8.60 | -18.00 | -16.39 | -12.59 |
Bud Site | -1.12 | -0.91 | -6.18 | -5.03 | -4.00 | -0.06 | 1.03 | -4.75 | -4.55 | -5.25 | -0.84 | 0.58 | -7.50 | -6.90 | -7.63 |
Cell Periphery | -0.68 | 3.62 | -3.72 | -3.34 | -4.44 | 1.13 | 1.16 | -3.10 | -4.01 | -4.08 | 0.43 | 2.25 | -4.44 | -4.54 | -5.01 |
Cytoplasm | 0.08 | 2.03 | -3.29 | -3.14 | -4.44 | 1.46 | 1.57 | 0.13 | -2.99 | -3.29 | 1.42 | 2.17 | -2.63 | -4.33 | -5.06 |
Cytoplasmic Foci | -1.44 | -1.35 | -5.83 | -4.71 | -5.46 | -1.26 | 1.71 | -4.72 | -4.10 | -5.05 | -1.77 | 1.63 | -7.36 | -6.24 | -7.77 |
Eisosomes | -4.06 | -2.02 | -7.52 | -1.89 | -4.09 | -0.97 | -2.29 | -4.39 | -4.93 | -1.76 | -2.69 | -3.65 | -6.27 | -4.58 | -1.06 |
Endoplasmic Reticulum | 4.43 | 3.88 | 6.64 | 1.71 | -0.71 | 5.43 | 7.74 | 11.60 | 9.07 | 7.03 | 7.49 | 11.24 | 10.88 | 4.12 | -0.10 |
Endosome | -0.33 | 2.41 | -4.03 | -3.85 | -4.62 | 0.71 | 2.67 | 0.22 | -0.45 | -2.47 | 0.54 | 3.09 | -1.80 | -2.66 | -5.22 |
Golgi | -0.79 | -0.06 | -2.11 | -1.44 | -2.17 | -1.21 | 3.80 | -1.80 | -0.79 | -2.23 | -1.38 | 2.82 | -2.85 | -1.61 | -3.48 |
Lipid Particles | 1.19 | 1.45 | 0.99 | -0.92 | -2.67 | -1.07 | 2.64 | -1.67 | 0.16 | -3.53 | 0.57 | 1.93 | 0.45 | -0.31 | -5.84 |
Mitochondria | -1.70 | -1.84 | -9.15 | -3.12 | -7.61 | -2.36 | -4.23 | -11.29 | -8.23 | -9.97 | -2.78 | -3.85 | -14.62 | -8.76 | -12.97 |
Mitotic Spindle | -0.94 | -2.89 | -5.89 | -4.80 | 0.42 | -1.52 | 0.66 | -4.24 | -2.66 | -4.49 | -1.76 | -1.35 | -7.38 | -5.63 | -6.31 |
None | -0.26 | 4.04 | -1.66 | -1.21 | -4.18 | 0.76 | 4.45 | 0.17 | -0.70 | -5.52 | 0.55 | 5.55 | -1.96 | -2.15 | -4.87 |
Nuclear Periphery | 1.18 | -3.69 | -8.00 | -8.83 | 0.73 | 2.23 | -5.05 | -9.02 | -10.43 | -5.28 | 2.52 | -6.37 | -12.03 | -13.71 | -5.78 |
Nuclear Periphery Foci | -0.73 | -3.16 | -3.18 | -1.57 | 1.90 | 4.71 | 7.61 | -3.53 | -8.00 | -10.67 | 3.27 | 5.87 | -4.16 | -7.28 | -10.29 |
Nucleolus | -5.44 | -12.08 | -0.28 | 5.21 | 11.93 | -1.04 | 8.46 | -4.07 | -3.08 | -11.23 | -4.66 | 3.17 | -1.27 | 3.02 | -4.06 |
Nucleus | 6.03 | 22.05 | 9.65 | 2.95 | -14.04 | 0.58 | -7.41 | 17.85 | 16.87 | 23.27 | 4.59 | -1.32 | 17.19 | 12.48 | 16.07 |
Peroxisomes | -3.39 | -7.21 | -8.10 | -5.02 | 4.68 | -1.12 | 2.39 | -10.61 | -11.00 | -13.76 | -2.69 | -1.78 | -12.94 | -11.98 | -12.17 |
Vacuole | -0.98 | 0.35 | -6.29 | -5.25 | -5.35 | -1.25 | 0.75 | -11.07 | -9.45 | -11.72 | -1.42 | 1.55 | -11.61 | -10.13 | -12.61 |
Vacuole Periphery | -0.75 | 0.58 | -9.83 | -9.31 | -10.73 | -2.13 | -10.25 | -15.45 | -14.64 | -12.10 | -1.90 | -9.10 | -18.49 | -17.52 | -14.08 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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