Standard name
Human Ortholog
Description Novel subunit of the vacuolar transporter chaperone complex; vacuolar transmembrane protein that regulates biosynthesis of polyphosphate; deletion reduces and overexpression increases polyP accumulation; SPX domain (Syg1, Pho81, Xpr1)-containing protein involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.27 0.55 0.84 0.82 0.92 0.94 0.94 0.91 – 0.92 0.92 0.48 0.79 0.93 0.23 0.19 0.3 0 0 0.11 0.09 0.11 0.17
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.07 0 0.07 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.33 0.24 0.05 0.05 0 0.07 0.07 0 0.16 – 0.17 0.09 0.05 0 0 0 0 0 0.06 0 0.07 0 0 0.05
Nucleus 0.09 0.06 0 0 0 0 0 0 0 – 0 0 0 0 0 0.13 0.15 0.14 0 0.11 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.63 0.57 0.43 0.25 0.29 0.08 0.06 0 0 – 0 0 0.55 0.3 0.07 0.69 0.73 0.67 0.79 0.68 0.76 0.81 0.78 0.64
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 1 1 0 1 2 1 1 0 4 – 6 7 0 1 1 1 0 0 3 2 1 0 0 8
Bud Neck 0 0 0 0 0 0 0 1 1 – 0 0 0 0 0 0 0 0 1 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 1 0 4 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 44 26 84 88 167 176 290 282 233 – 229 223 93 202 250 61 40 50 5 4 23 10 22 49
Endoplasmic Reticulum 5 0 1 0 1 0 0 1 1 – 0 0 0 0 0 6 9 5 3 0 0 2 2 7
Endosome 2 0 1 0 0 1 0 0 0 – 0 1 3 8 1 9 12 11 9 8 3 1 3 9
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 2 1 2 2 0 0 0 1 2
Mitochondria 72 23 8 5 7 13 21 10 42 – 42 21 10 1 1 7 2 3 15 2 16 3 8 15
Nucleus 19 6 5 1 3 5 2 3 4 – 0 4 3 0 0 35 31 23 2 14 2 0 1 0
Nuclear Periphery 2 1 0 0 0 5 1 0 3 – 1 2 0 0 0 0 1 1 2 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 1 – 0 0 0 0 0 0 0 0 0 1 0 0 0 2
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 1 1 0 0 – 0 0 0 0 0 0 2 0 3 1 0 0 1 3
Vac/Vac Membrane 139 54 66 26 59 16 19 11 11 – 2 5 107 77 18 180 151 110 196 89 165 92 159 186
Unique Cell Count 220 95 152 105 204 192 308 299 256 250 242 195 257 269 261 207 164 249 132 218 114 205 290
Labelled Cell Count 285 111 166 121 239 219 336 313 300 281 263 216 289 272 301 249 205 249 132 218 114 205 290


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 4.8 5.0 4.7 5.3 4.2 4.1 4.4 3.6 – 3.3 3.8 5.0 4.6 4.4 7.8 7.7 7.6 4.5 4.2 4.4
Std Deviation (1e-4) 1.2 0.8 1.2 1.5 1.5 1.8 1.3 1.7 1.1 – 1.3 1.0 1.5 1.4 1.7 1.9 1.6 1.6 1.8 1.7 1.9
Intensity Change (Log2) – – – -0.08 0.1 -0.23 -0.27 -0.17 -0.45 – -0.58 -0.41 0.01 -0.11 -0.17 0.65 0.62 0.62 -0.14 -0.25 -0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.1 5.7 4.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2917 2.3374 1.9035 1.0121 2.2234 1.6049 -0.9761 -0.6774 -0.6184 -0.4174 -0.6799 -0.668 -0.4874 0.2772 -0.2104 0.0049 0.1071 -0.2024
Actin 0.0139 0.0001 0.0023 0.0002 0.0195 0.0031 0.0313 0 0.0036 0.0401 0.0002 0.0008 0.0088 0.0001 0.0004 0 0.0003 0.0001
Bud 0.0009 0.0015 0.0006 0.001 0.0014 0.0006 0.002 0.0007 0.0032 0.0012 0.0005 0.0011 0.0041 0.0108 0.0023 0.0036 0.0014 0.0019
Bud Neck 0.0038 0.0012 0.0022 0.0011 0.0116 0.0116 0.0036 0.0014 0.0016 0.017 0.001 0.0038 0.0059 0.0014 0.002 0.0016 0.0018 0.004
Bud Periphery 0.0028 0.0028 0.0018 0.0045 0.0056 0.0018 0.0024 0.0009 0.0035 0.0022 0.0008 0.0023 0.004 0.0059 0.0021 0.0016 0.0012 0.0018
Bud Site 0.0112 0.0071 0.0036 0.001 0.007 0.0015 0.0169 0.0035 0.0097 0.0068 0.001 0.0012 0.0091 0.0243 0.0082 0.001 0.002 0.0009
Cell Periphery 0.0032 0.0016 0.0016 0.0016 0.0022 0.0021 0.0009 0.001 0.0011 0.0005 0.0004 0.0005 0.001 0.0015 0.0012 0.0008 0.0006 0.0006
Cytoplasm 0.0652 0.0604 0.0588 0.0279 0.0384 0.0669 0.0931 0.1361 0.0893 0.0479 0.0492 0.1204 0.0738 0.0969 0.0665 0.0292 0.0448 0.0716
Cytoplasmic Foci 0.0508 0.0448 0.0506 0.0403 0.053 0.0454 0.0834 0.067 0.0502 0.1198 0.0968 0.0649 0.082 0.0513 0.0652 0.0899 0.1102 0.0978
Eisosomes 0.001 0.0004 0.0006 0.0001 0.0013 0.0001 0.0003 0 0.0002 0.0001 0 0 0.0003 0 0.0001 0 0 0
Endoplasmic Reticulum 0.012 0.004 0.0051 0.0033 0.0023 0.0049 0.0086 0.0048 0.0064 0.0022 0.0029 0.0055 0.0069 0.0047 0.0071 0.002 0.0016 0.0032
Endosome 0.1918 0.1393 0.2067 0.1922 0.1149 0.2071 0.3057 0.2885 0.3358 0.4025 0.442 0.2996 0.2957 0.1939 0.3456 0.3787 0.3507 0.2928
Golgi 0.0073 0.0032 0.0058 0.0079 0.0126 0.0093 0.0155 0.004 0.0138 0.0119 0.0087 0.0111 0.0068 0.0028 0.0091 0.0037 0.009 0.0036
Lipid Particles 0.0497 0.027 0.0429 0.0417 0.0939 0.0211 0.0321 0.0045 0.0094 0.0109 0.0196 0.013 0.0177 0.0068 0.0161 0.0615 0.0118 0.0112
Mitochondria 0.0221 0.0227 0.0484 0.0084 0.0721 0.0317 0.0249 0.0055 0.0339 0.0072 0.0318 0.0103 0.0194 0.0057 0.0413 0.0048 0.0067 0.0043
None 0.3392 0.4012 0.3214 0.2093 0.2604 0.1422 0.1196 0.1052 0.1134 0.0231 0.0135 0.0643 0.1776 0.171 0.1285 0.0407 0.0712 0.0927
Nuclear Periphery 0.0133 0.0025 0.0052 0.0024 0.0044 0.0056 0.0131 0.0043 0.0093 0.0025 0.0046 0.0093 0.0098 0.0047 0.0101 0.0051 0.0032 0.0038
Nucleolus 0.0047 0.0038 0.0059 0.0032 0.0156 0.0039 0.0057 0.0062 0.0032 0.0031 0.0023 0.0027 0.0036 0.0063 0.0032 0.0111 0.0033 0.0031
Nucleus 0.0181 0.0101 0.0066 0.0051 0.0092 0.0101 0.0094 0.0088 0.0057 0.0046 0.017 0.0103 0.0059 0.0094 0.0126 0.0116 0.005 0.0077
Peroxisomes 0.0065 0.002 0.015 0.0264 0.0387 0.0035 0.0096 0.0014 0.003 0.0354 0.0019 0.0025 0.0032 0.0011 0.0041 0.0027 0.0037 0.0017
Punctate Nuclear 0.0032 0.0009 0.0118 0.001 0.0069 0.001 0.0044 0.001 0.0013 0.0019 0.0012 0.0036 0.0033 0.0035 0.0029 0.0134 0.0098 0.006
Vacuole 0.163 0.2305 0.1787 0.3793 0.1984 0.3768 0.1875 0.3177 0.2607 0.2174 0.2652 0.3261 0.2283 0.3645 0.2223 0.281 0.3285 0.3469
Vacuole Periphery 0.0164 0.0329 0.0245 0.0421 0.0304 0.0498 0.0301 0.0371 0.0419 0.0417 0.0393 0.0466 0.0329 0.0334 0.0492 0.056 0.0332 0.0442

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.699 12.8493 10.3761 15.0786 10.4144 10.5632 21.2075 19.0848 16.5587 17.2391
Translational Efficiency 0.558 1.0523 0.7825 0.5724 0.8865 0.7282 0.7883 0.7234 0.8753 0.7838

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1623 1196 339 1404 2347 1638 189 1035 3970 2834 528 2439

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 699.06 776.25 960.61 852.99 781.53 717.91 937.96 754.12 747.81 742.53 952.50 811.03
Standard Deviation 84.67 102.64 107.46 102.54 91.81 135.51 89.02 108.39 97.76 126.06 101.83 115.87
Intensity Change Log 2 — 0.151105 0.458535 0.287113 — -0.122498 0.263225 -0.051507 — 0.013162 0.358741 0.118296

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000033 0.000120 0.000952 0.000848 0.000017 0.000130 0.000343 0.000376 0.000024 0.000126 0.000734 0.000647
Bud Neck 0.016891 0.019044 0.004804 0.009043 0.008950 0.029720 0.002485 0.008098 0.012196 0.025215 0.003974 0.008642
Bud Site 0.007695 0.014760 0.035385 0.040492 0.003321 0.015222 0.002786 0.056154 0.005109 0.015027 0.023716 0.047138
Cell Periphery 0.000233 0.000125 0.000214 0.000120 0.000119 0.000109 0.000175 0.000299 0.000166 0.000116 0.000200 0.000196
Cytoplasm 0.108770* 0.120258* 0.145103* 0.430460* 0.039032 0.147271* 0.214440* 0.453934* 0.067542 0.135871* 0.169922* 0.440421*
Cytoplasmic Foci 0.043631 0.070064 0.004913 0.036676 0.016899 0.104558 0.011628 0.041684 0.027828 0.090001 0.007317 0.038801
Eisosomes 0.000042 0.000049 0.000097 0.000041 0.000018 0.000064 0.000113 0.000058 0.000028 0.000058 0.000102 0.000048
Endoplasmic Reticulum 0.004603 0.002082 0.015138 0.003396 0.005408 0.002098 0.007348 0.001336 0.005079 0.002091 0.012349 0.002522
Endosome 0.067760 0.084730 0.004037 0.015133 0.036365 0.105236 0.006252 0.017215 0.049200 0.096582 0.004830 0.016016
Golgi 0.001174 0.003864 0.000125 0.008299 0.000568 0.004632 0.000100 0.000703 0.000816 0.004308 0.000116 0.005075
Lipid Particles 0.004493 0.002894 0.003337 0.002225 0.001943 0.006522 0.004127 0.002720 0.002985 0.004991 0.003620 0.002435
Mitochondria 0.003674 0.005605 0.001204 0.004593 0.001157 0.008308 0.000371 0.002946 0.002186 0.007167 0.000906 0.003894
Mitotic Spindle 0.000154 0.004166 0.000783 0.050662 0.000942 0.004634 0.008411 0.008196 0.000620 0.004436 0.003513 0.032641
None 0.001232 0.000584 0.004338 0.004074 0.000242 0.001073 0.005669 0.019386 0.000647 0.000867 0.004814 0.010572
Nuclear Periphery 0.000526 0.000331 0.003156 0.002484 0.000876 0.000827 0.002931 0.001197 0.000733 0.000618 0.003076 0.001938
Nuclear Periphery Foci 0.000176 0.000252 0.004080 0.002952 0.000254 0.000350 0.002788 0.001226 0.000222 0.000309 0.003617 0.002220
Nucleolus 0.001563 0.001352 0.001318 0.001771 0.001021 0.001729 0.000764 0.001955 0.001242 0.001570 0.001120 0.001849
Nucleus 0.030053 0.019621 0.620274* 0.126749 0.021408 0.035251 0.552949* 0.136783* 0.024943 0.028655 0.596175* 0.131007*
Peroxisomes 0.001645 0.003515 0.000411 0.002916 0.000673 0.005700 0.000486 0.001162 0.001070 0.004778 0.000438 0.002172
Vacuole 0.685921* 0.627652* 0.149569 0.252390* 0.845568* 0.512081* 0.175610 0.242911* 0.780302* 0.560854* 0.158890 0.248367*
Vacuole Periphery 0.019731 0.018930 0.000764 0.004678 0.015219 0.014485 0.000224 0.001661 0.017064 0.016361 0.000571 0.003398

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.96 -14.91 -4.43 -3.35 5.62 -6.20 -10.49 -5.28 -3.16 1.95 -7.26 -16.92 -6.27 -4.37 6.23
Bud Neck -1.10 9.11 6.48 6.50 -2.84 -10.89 5.82 2.33 12.49 -4.03 -9.45 9.96 5.82 13.67 -4.37
Bud Site -4.29 -1.46 -4.93 -2.18 -1.23 -9.83 -0.79 -8.34 -5.31 -8.18 -10.08 -1.88 -9.98 -5.42 -4.71
Cell Periphery 2.68 1.18 3.86 3.78 7.87 0.91 -1.72 -2.81 -3.17 -1.91 2.81 -0.24 1.61 -1.15 2.50
Cytoplasm -1.66 1.07 -30.67 -28.04 -24.96 -21.17 -9.40 -39.16 -24.59 -15.47 -16.39 -7.14 -51.82 -36.89 -29.10
Cytoplasmic Foci -6.97 19.27 8.21 13.24 -14.46 -24.47 5.59 -7.16 18.72 -11.20 -23.59 19.00 0.22 23.63 -18.38
Eisosomes -1.49 -11.11 0.78 1.98 11.77 -11.76 -11.65 -12.57 1.61 6.92 -9.49 -16.81 -9.75 3.50 13.35
Endoplasmic Reticulum 6.17 -13.53 4.15 -2.40 15.17 6.84 -4.54 9.64 0.85 10.34 9.04 -13.37 8.99 -1.50 17.24
Endosome -3.93 24.45 22.48 22.14 -4.83 -18.90 20.32 13.73 26.27 -4.85 -16.96 32.21 26.66 34.79 -6.93
Golgi -4.18 4.51 -1.60 0.98 -2.72 -5.49 2.51 0.51 5.65 -1.65 -6.90 4.78 -1.65 3.37 -2.93
Lipid Particles 3.33 2.50 7.16 2.78 5.03 -8.10 -3.61 -1.73 7.26 2.41 -5.30 -1.45 4.26 8.02 5.30
Mitochondria -1.73 3.58 1.67 3.34 -1.54 -7.18 3.65 -0.76 5.09 -1.71 -6.69 4.19 0.42 6.20 -2.21
Mitotic Spindle -3.47 -4.79 -8.83 -7.13 -8.43 -2.85 -1.43 -6.18 -3.47 -2.12 -4.26 -1.95 -10.38 -7.84 -8.40
None 1.57 -3.20 -3.85 -6.28 -0.93 -1.79 -4.44 -6.13 -5.57 -4.61 -0.70 -6.34 -7.38 -6.95 -4.62
Nuclear Periphery 3.01 -19.64 -16.38 -17.77 3.46 0.19 -9.42 -5.34 -5.38 6.73 0.72 -18.29 -13.58 -14.18 6.99
Nuclear Periphery Foci -1.33 -5.63 -10.68 -10.07 1.90 -1.04 -7.59 -8.46 -7.88 4.01 -1.47 -7.69 -13.05 -12.36 3.23
Nucleolus 0.46 1.80 0.70 0.10 -1.08 -2.79 2.81 -1.66 1.05 -4.56 -1.56 2.23 -0.72 0.89 -2.94
Nucleus 3.94 -38.93 -26.85 -29.75 29.31 -5.43 -21.87 -21.32 -18.15 15.49 -2.19 -43.39 -34.96 -32.94 32.00
Peroxisomes -4.13 4.33 0.91 4.32 -1.96 -7.08 3.58 -0.39 6.98 -3.90 -8.21 5.50 0.32 7.65 -2.74
Vacuole 5.23 33.58* 31.59 23.46 -9.81 38.25* 26.62 50.53* 15.40 -1.38 31.06* 46.61* 60.54* 26.13 -8.15
Vacuole Periphery 0.69 22.21 18.16 16.88 -2.56 1.04 23.30 20.31 17.60 -3.10 1.15 31.89 25.97 23.38 -3.46
Standard name
Human Ortholog
Description Novel subunit of the vacuolar transporter chaperone complex; vacuolar transmembrane protein that regulates biosynthesis of polyphosphate; deletion reduces and overexpression increases polyP accumulation; SPX domain (Syg1, Pho81, Xpr1)-containing protein involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress
Localization
Cell Percentages vacuole (95%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vtc5

Vtc5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vtc5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available