Standard name
Human Ortholog
Description Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF2 has a paralog, DNF1, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.52 0.57 0.5 0.47 0.36 0.26 0.09 0.11 – 0.06 0.05 0.1 0.46 0.47 0.31 0.39 0.4 0.35 0.29 0.3 0.36 0.25 0.25 0.26
Bud 0.17 0.18 0.18 0.17 0.19 0.17 0.17 0.13 – 0.11 0.11 0.15 0.19 0.19 0.14 0.13 0.2 0.14 0.27 0.3 0.16 0.31 0.29 0.26
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.15 0.13 0.12 0.21 0.16 0.15
Bud Site 0.11 0.18 0.12 0.06 0.05 0.08 0 0.05 – 0 0.05 0 0 0 0 0.13 0.09 0.06 – – – – – –
Cell Periphery 0.17 0.14 0.22 0.13 0.12 0.06 0.05 0.07 – 0.06 0.14 0.07 0.27 0.27 0.28 0.18 0.15 0.25 0.1 0 0.13 0 0.09 0.09
Cytoplasm 0.1 0.11 0.17 0.28 0.24 0.29 0.41 0.5 – 0.56 0.5 0.44 0.18 0.18 0.25 0.33 0.3 0.35 0 0 0 0 0 0
Endoplasmic Reticulum 0.21 0.15 0.15 0.21 0.21 0.17 0.07 0.06 – 0 0 0 0.26 0.27 0.3 0.05 0.07 0.08 0.09 0.07 0.1 0.08 0.11 0.11
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.19 0 0.2 0.12 0.3 0.33 0.25 – 0.34 0.33 0.43 0 0 0 0.11 0.08 0.06 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0 0.07 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0.06 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 172 125 82 42 44 51 19 26 – 17 7 21 121 123 87 154 143 55 139 84 77 144 127 166
Bud 56 39 29 15 23 33 35 30 – 31 14 32 51 51 39 50 72 22 127 85 34 177 147 167
Bud Neck 1 0 0 0 0 0 0 0 – 1 1 0 0 0 0 0 1 0 70 36 25 121 80 97
Bud Site 35 39 19 5 6 16 6 13 – 10 7 8 6 7 2 52 33 10 – – – – – –
Cell Periphery 57 31 37 12 15 12 11 16 – 16 18 14 70 70 79 70 52 39 49 12 27 21 43 61
Cytoplasm 33 24 28 25 29 58 83 118 – 161 66 94 48 48 70 128 109 55 8 3 1 6 6 17
Endoplasmic Reticulum 69 33 24 19 26 34 15 15 – 11 5 8 69 72 85 18 25 13 42 20 22 47 55 74
Endosome 4 2 1 0 3 3 4 8 – 3 3 3 1 1 3 1 4 4 7 7 3 5 1 5
Golgi 3 0 1 0 1 0 0 0 – 0 0 0 3 3 2 3 1 1 4 2 2 2 0 3
Mitochondria 22 41 6 18 14 60 67 59 – 98 43 91 0 0 2 44 30 10 11 12 9 21 20 24
Nucleus 8 2 7 0 3 2 4 8 – 7 4 3 0 0 0 26 16 8 1 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 1 4 2 – 1 0 3 0 0 0 0 1 0 1 0 1 1 0 4
Nucleolus 4 1 2 0 0 1 2 7 – 1 1 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 13 5 8 4 8 9 2 2 – 3 0 3 3 3 2 3 6 0 0 0 1 0 0 0
SpindlePole 2 0 5 1 0 1 2 2 – 1 2 1 6 4 5 19 19 8 1 0 0 1 0 1
Vac/Vac Membrane 7 9 3 0 6 1 12 9 – 6 2 8 5 3 12 9 12 7 9 12 2 15 11 16
Unique Cell Count 328 219 165 90 121 198 203 238 287 131 212 263 264 285 391 358 157 480 284 214 573 502 651
Labelled Cell Count 487 351 252 141 178 282 266 315 367 173 290 383 385 388 577 525 232 480 284 214 573 502 651


Ambiguous, Bud, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.6 8.6 8.0 7.7 7.2 5.9 5.6 5.7 – 4.8 4.9 4.9 9.9 9.9 10.2 7.9 8.7 9.7 9.3 9.6 9.8
Std Deviation (1e-4) 2.1 1.9 1.8 1.9 2.0 1.7 1.4 1.6 – 1.0 1.1 1.2 2.4 2.4 2.7 1.9 2.0 2.4 1.8 1.8 1.8
Intensity Change (Log2) – – – -0.06 -0.15 -0.43 -0.51 -0.48 – -0.74 -0.7 -0.71 0.3 0.3 0.35 -0.02 0.13 0.28 0.22 0.26 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.5 -2.2 -4.7 -8.6 -8.6 – 0 -8.3 0 -0.7 -0.6 -4.0 -2.2 -2.1 -2.7
Bud -0.2 0.3 -0.2 -0.1 -1.4 – 0 -1.7 0 0.5 0.5 -1.1 -1.5 0.7 -0.9
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site -1.6 -1.9 -1.1 -3.2 -2.2 – 0 -1.9 0 -4.0 -3.7 -5.2 0.6 -0.8 -1.6
Cell Periphery -1.8 -2.2 -4.5 -4.8 -4.6 – 0 -1.9 0 1.0 1.0 1.2 -1.2 -2.2 0.5
Cytoplasm 2.0 1.5 2.7 5.0 6.7 – 8.1 6.1 5.6 0.3 0.3 1.9 3.8 3.3 3.7
Endoplasmic Reticulum 1.3 1.5 0.7 -2.2 -2.8 – 0 -3.1 0 2.9 3.1 3.6 -4.1 -2.8 -1.8
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 4.3 2.6 6.6 7.0 5.7 – 0 6.7 0 0 0 0 2.9 2.0 1.1
Nucleus 0 0 0 0 -0.5 – -1.1 0 0 0 0 0 1.1 0.1 0.4
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.6 -0.1 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 1.0 1.2 0.9
Vacuole 0 0 0 2.0 0 – 0 0 0 0 0 1.4 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.7727 4.8054 5.4941 4.9185 5.7301 5.286 7.6532 7.9309 8.0043 7.9621 7.926 8.0684 7.7519 6.9226 7.8315 7.4037 7.4229 7.0822
Actin 0.122 0.1329 0.119 0.1976 0.1887 0.1499 0.109 0.0579 0.0831 0.0452 0.2983 0.1043 0.0542 0.005 0.154 0.1094 0.4264 0.1349
Bud 0.0348 0.0237 0.2632 0.0861 0.1183 0.0133 0.0303 0.0395 0.2158 0.0745 0.0497 0.0105 0.0019 0.0056 0.2339 0.1032 0.1115 0.0055
Bud Neck 0.0747 0.0208 0.0115 0.0512 0.0117 0.4831 0.0521 0.009 0.0127 0.0094 0.0641 0.5914 0.1924 0.0156 0.0009 0.0009 0.002 0.5991
Bud Periphery 0.1083 0.0326 0.3658 0.4338 0.4913 0.0498 0.0962 0.066 0.4527 0.733 0.293 0.0657 0.0103 0.0048 0.4732 0.4104 0.4125 0.0314
Bud Site 0.0784 0.4204 0.0719 0.0142 0.0249 0.0189 0.2254 0.5626 0.1101 0.0161 0.0145 0.0349 0.0603 0.6443 0.0509 0.0358 0.0105 0.0086
Cell Periphery 0.1059 0.0259 0.0105 0.035 0.0748 0.034 0.082 0.0288 0.0124 0.0727 0.0312 0.0599 0.0714 0.0105 0.0018 0.003 0.0112 0.0378
Cytoplasm 0.1785 0.1852 0.0301 0.0392 0.0423 0.1092 0.1067 0.1536 0.0492 0.012 0.0275 0.0845 0.0151 0.1327 0.0266 0.008 0.0043 0.0793
Cytoplasmic Foci 0.0224 0.0353 0.0268 0.0338 0.0015 0.0278 0.0515 0.0157 0.0154 0.0003 0.0284 0.0064 0.1076 0.0153 0.0033 0.0373 0.001 0.0272
Eisosomes 0.0011 0.001 0.0003 0.0016 0.0003 0.0006 0.0019 0.0006 0.0003 0.0012 0.0012 0.0006 0.0045 0.0001 0.0003 0.0001 0.0007 0.0007
Endoplasmic Reticulum 0.0891 0.0187 0.0174 0.0045 0.0232 0.0137 0.0115 0.0049 0.001 0.0073 0.0059 0.0049 0.0109 0.0043 0.0166 0.0033 0.0024 0.0097
Endosome 0.0295 0.0358 0.0161 0.0345 0.0013 0.0298 0.0703 0.0188 0.0087 0.0006 0.0366 0.0045 0.2738 0.1065 0.0099 0.1147 0.002 0.0212
Golgi 0.0216 0.0417 0.0238 0.0485 0.0024 0.0381 0.0502 0.0165 0.0107 0.0006 0.0524 0.0066 0.0312 0.0202 0.0081 0.0324 0.0069 0.0206
Lipid Particles 0.0309 0.006 0.0048 0.0047 0.0006 0.0081 0.0303 0.0017 0.0017 0.0002 0.0143 0.0043 0.0821 0.0052 0.0008 0.0487 0.0005 0.0125
Mitochondria 0.0029 0.0025 0.014 0.0032 0.0024 0.0065 0.0157 0.0032 0.0022 0.0012 0.0642 0.0015 0.0136 0.0015 0.0016 0.0105 0.003 0.0018
None 0.002 0.002 0.0007 0.0004 0.0005 0.0006 0.0033 0.0106 0.0007 0.0006 0.0009 0.0064 0.0074 0.0004 0.0007 0.0028 0.0004 0.0005
Nuclear Periphery 0.0046 0.0012 0.0019 0.0007 0.0029 0.0014 0.007 0.0007 0.0006 0.0014 0.0006 0.0006 0.0089 0.0012 0.0079 0.0265 0.0013 0.0004
Nucleolus 0.001 0.0006 0.0003 0.0002 0.0002 0.0004 0.0027 0.0008 0.0005 0.0002 0.0005 0.0007 0.0027 0.0016 0.0001 0.0008 0.0001 0.0003
Nucleus 0.0033 0.0011 0.001 0.0005 0.0009 0.0014 0.0026 0.0011 0.0011 0.0011 0.0007 0.0013 0.0025 0.0011 0.0038 0.0024 0.0005 0.0005
Peroxisomes 0.001 0.0016 0.0064 0.0046 0.0003 0.0047 0.0283 0.0007 0.0056 0.0001 0.0065 0.004 0.0054 0.0016 0.0012 0.0224 0.0004 0.0038
Punctate Nuclear 0.0534 0.0007 0.0008 0.0008 0.0001 0.001 0.0018 0.0004 0.0017 0.0001 0.0005 0.0021 0.0202 0.0004 0.0005 0.0216 0.0002 0.0008
Vacuole 0.0314 0.0095 0.0113 0.004 0.0106 0.0066 0.0159 0.0063 0.012 0.0212 0.008 0.0045 0.016 0.0198 0.0031 0.0031 0.0019 0.0029
Vacuole Periphery 0.0032 0.0007 0.0022 0.0009 0.0007 0.0011 0.0054 0.0006 0.0015 0.0011 0.0011 0.0003 0.0075 0.0023 0.0006 0.0027 0.0006 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 57.1198 41.3793 41.142 41.5761 47.1549 55.379 56.0595 50.0776 55.8745 56.4925
Translational Efficiency 0.6319 0.5411 0.4586 0.5108 0.435 0.6202 0.4381 0.3808 0.3474 0.4443

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF2 has a paralog, DNF1, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (43%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-3

Dnf2

Dnf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dnf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available