Standard name | |
---|---|
Human Ortholog | |
Description | Glutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; with Grx4p, binds to Aft1p in iron-replete conditions, promoting its dissociation from promoters; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36; GRX3 has a paralog, GRX4, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 3 |
Bud | 1 | 0 | 0 | 1 | 6 | 2 | 3 | 3 | 12 | 11 | 7 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 5 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 2 | 3 | 3 | 7 | 7 | 5 | 5 | 9 | 5 | 11 | 1 | 1 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 223 | 393 | 486 | 369 | 486 | 691 | 863 | 874 | 654 | 627 | 498 | 647 | 173 | 340 | 335 | 134 | 112 | 96 | 216 | 390 | 471 | 202 | 235 | 207 |
Endoplasmic Reticulum | 1 | 4 | 2 | 1 | 2 | 0 | 1 | 3 | 1 | 0 | 2 | 3 | 0 | 1 | 1 | 4 | 6 | 2 | 0 | 0 | 0 | 2 | 2 | 2 |
Endosome | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 6 | 4 | 7 | 6 | 8 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 3 | 0 | 2 | 1 |
Mitochondria | 1 | 6 | 0 | 5 | 9 | 31 | 32 | 26 | 225 | 105 | 158 | 181 | 0 | 0 | 0 | 5 | 0 | 1 | 1 | 6 | 3 | 2 | 2 | 6 |
Nucleus | 2 | 1 | 3 | 1 | 2 | 9 | 15 | 21 | 10 | 15 | 22 | 16 | 1 | 2 | 2 | 4 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 3 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 | 2 |
Vac/Vac Membrane | 0 | 13 | 3 | 10 | 19 | 59 | 95 | 99 | 57 | 80 | 50 | 124 | 5 | 25 | 11 | 12 | 3 | 8 | 1 | 2 | 1 | 0 | 2 | 5 |
Unique Cell Count | 227 | 412 | 489 | 378 | 510 | 759 | 963 | 992 | 828 | 766 | 671 | 883 | 175 | 352 | 339 | 146 | 113 | 103 | 229 | 420 | 495 | 227 | 268 | 253 |
Labelled Cell Count | 230 | 421 | 497 | 390 | 530 | 801 | 1020 | 1039 | 966 | 851 | 746 | 996 | 181 | 372 | 350 | 162 | 126 | 110 | 229 | 420 | 495 | 227 | 268 | 253 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.5 | 6.2 | 7.4 | 7.2 | 6.5 | 6.5 | 6.4 | 6.6 | 5.1 | 5.8 | 5.3 | 5.6 | 8.4 | 8.2 | 8.0 | 7.1 | 9.1 | 9.9 | 8.0 | 8.4 | 8.6 |
Std Deviation (1e-4) | 1.0 | 0.9 | 1.2 | 1.1 | 1.5 | 1.3 | 1.2 | 1.3 | 1.1 | 0.9 | 1.0 | 1.0 | 1.3 | 1.2 | 1.5 | 1.5 | 1.6 | 2.5 | 1.4 | 1.7 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.2 | -0.19 | -0.21 | -0.18 | -0.53 | -0.36 | -0.48 | -0.4 | 0.17 | 0.14 | 0.1 | -0.06 | 0.3 | 0.41 | 0.1 | 0.17 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.2 | -4.0 | -6.2 | -6.9 | -7.5 | -10.5 | -9.6 | -11.8 | -12.2 | -0.7 | -3.0 | -0.9 | -5.3 | -0.3 | -4.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.5 | 4.1 | 3.6 | 12.7 | 8.6 | 11.5 | 10.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 1.5 | 2.2 | 0 | 2.0 | 3.1 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.4 | 3.4 | 5.7 | 6.6 | 6.7 | 5.3 | 6.8 | 5.5 | 8.2 | 0 | 5.2 | 2.9 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.1157 | 7.8099 | 6.9285 | 6.5048 | 6.3805 | 6.8378 | 9.5742 | 11.2047 | 10.5381 | 10.1404 | 9.917 | 10.402 | 8.343 | 9.8186 | 8.7906 | 8.4071 | 8.1116 | 8.7135 |
Actin | 0.0306 | 0.0172 | 0.0155 | 0.0008 | 0.0004 | 0.0005 | 0.0321 | 0.0002 | 0.0171 | 0.0008 | 0.0623 | 0.0004 | 0.0043 | 0.0003 | 0.0029 | 0.0014 | 0.0179 | 0.0017 |
Bud | 0.0006 | 0.0014 | 0.0026 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0005 | 0.0013 | 0.0002 | 0.0025 | 0.0004 | 0.0003 |
Bud Neck | 0.0053 | 0.0005 | 0.0013 | 0.0009 | 0.0006 | 0.0015 | 0.0069 | 0.0002 | 0.0006 | 0.0004 | 0.0023 | 0.0017 | 0.0011 | 0.0004 | 0.0006 | 0.0008 | 0.0011 | 0.0016 |
Bud Periphery | 0.0005 | 0.0006 | 0.001 | 0.0002 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0.001 | 0 | 0.0004 | 0.0003 | 0.0001 | 0.0041 | 0.0006 | 0.0002 |
Bud Site | 0.004 | 0.0008 | 0.0019 | 0.0002 | 0.0001 | 0.0001 | 0.0047 | 0.0002 | 0.0014 | 0.0001 | 0.0004 | 0.0001 | 0.001 | 0.0068 | 0.0003 | 0.0085 | 0.0009 | 0.0001 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 |
Cytoplasm | 0.8138 | 0.9014 | 0.8521 | 0.9133 | 0.9573 | 0.9146 | 0.8195 | 0.9703 | 0.9028 | 0.924 | 0.8926 | 0.9194 | 0.8315 | 0.9021 | 0.9157 | 0.8297 | 0.7992 | 0.8685 |
Cytoplasmic Foci | 0.0215 | 0.0051 | 0.0081 | 0.0022 | 0.0025 | 0.0051 | 0.0264 | 0.0009 | 0.0035 | 0.0016 | 0.0029 | 0.0024 | 0.0277 | 0.0019 | 0.0034 | 0.0025 | 0.0399 | 0.0043 |
Eisosomes | 0.0008 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.005 | 0.0022 | 0.0027 | 0.0018 | 0.003 | 0.0023 | 0.0055 | 0.0013 | 0.0027 | 0.0021 | 0.0065 | 0.0024 | 0.0034 | 0.0019 | 0.0029 | 0.0019 | 0.0025 | 0.0018 |
Endosome | 0.0114 | 0.0087 | 0.005 | 0.0035 | 0.0017 | 0.0015 | 0.0138 | 0.0005 | 0.0059 | 0.0006 | 0.005 | 0.0013 | 0.0172 | 0.0034 | 0.0036 | 0.0028 | 0.0148 | 0.0057 |
Golgi | 0.0042 | 0.0016 | 0.0041 | 0.0025 | 0 | 0.0001 | 0.0046 | 0 | 0.0027 | 0.0001 | 0.0036 | 0.0001 | 0.0051 | 0.0003 | 0.0011 | 0.0004 | 0.0096 | 0.0015 |
Lipid Particles | 0.0044 | 0.0001 | 0.0019 | 0.0014 | 0 | 0 | 0.0069 | 0 | 0.0028 | 0 | 0.0005 | 0 | 0.0023 | 0.0003 | 0.0006 | 0.0003 | 0.0194 | 0.0012 |
Mitochondria | 0.0032 | 0.0024 | 0.013 | 0.0282 | 0.0001 | 0.0002 | 0.0038 | 0.0001 | 0.0008 | 0.0001 | 0.0014 | 0.0001 | 0.0097 | 0.0002 | 0.0014 | 0.0023 | 0.0069 | 0.0009 |
None | 0.0352 | 0.0018 | 0.0104 | 0.003 | 0.0085 | 0.0065 | 0.0205 | 0.0016 | 0.0043 | 0.0415 | 0.0017 | 0.0062 | 0.0102 | 0.0039 | 0.0045 | 0.0312 | 0.0059 | 0.0093 |
Nuclear Periphery | 0.0053 | 0.0028 | 0.0087 | 0.0032 | 0.0044 | 0.003 | 0.0076 | 0.0014 | 0.0041 | 0.0027 | 0.0066 | 0.0033 | 0.0084 | 0.0027 | 0.0032 | 0.0031 | 0.0061 | 0.0041 |
Nucleolus | 0.0008 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0008 | 0.0002 | 0.0001 |
Nucleus | 0.0285 | 0.0493 | 0.0604 | 0.0349 | 0.0195 | 0.0625 | 0.0324 | 0.0225 | 0.0408 | 0.0245 | 0.0088 | 0.0604 | 0.0585 | 0.0719 | 0.0543 | 0.0995 | 0.0443 | 0.0904 |
Peroxisomes | 0.0136 | 0.0001 | 0.008 | 0.0007 | 0 | 0.0001 | 0.0051 | 0 | 0.0076 | 0.0001 | 0.0002 | 0 | 0.0091 | 0.0001 | 0.0028 | 0.0004 | 0.0243 | 0.0014 |
Punctate Nuclear | 0.0097 | 0.0017 | 0.0019 | 0.0015 | 0.0012 | 0.0011 | 0.0067 | 0.0004 | 0.0018 | 0.001 | 0.0017 | 0.0016 | 0.0081 | 0.0011 | 0.0017 | 0.0019 | 0.0048 | 0.0061 |
Vacuole | 0.0012 | 0.0013 | 0.0008 | 0.0009 | 0.0004 | 0.0006 | 0.001 | 0.0003 | 0.0005 | 0.0002 | 0.0006 | 0.0004 | 0.0009 | 0.0011 | 0.0004 | 0.0049 | 0.0007 | 0.0004 |
Vacuole Periphery | 0.0003 | 0.0002 | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0008 | 0.0004 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 90.6644 | 71.2766 | 87.0131 | 87.0575 | 95.8744 | 95.1127 | 85.1445 | 82.7679 | 85.3845 | 96.0615 |
Translational Efficiency | 2.4647 | 2.6111 | 1.7235 | 2.1395 | 2.1613 | 2.5653 | 1.8524 | 1.953 | 1.9383 | 1.882 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1036 | 670 | 2646 | 1366 | 1833 | 2276 | 2726 | 1590 | 2869 | 2946 | 5372 | 2956 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 707.50 | 909.18 | 1008.98 | 1203.32 | 754.99 | 953.50 | 1062.69 | 1237.49 | 737.84 | 943.42 | 1036.23 | 1221.70 |
Standard Deviation | 84.57 | 122.34 | 129.18 | 170.13 | 98.27 | 118.09 | 147.11 | 184.90 | 96.30 | 120.51 | 141.15 | 179.04 |
Intensity Change Log 2 | — | 0.361836 | 0.512096 | 0.766218 | — | 0.336775 | 0.493191 | 0.712887 | — | 0.348953 | 0.502367 | 0.738933 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000078 | 0.000508 | 0.000329 | 0.006651 | 0.000080 | 0.000224 | 0.000184 | 0.005584 | 0.000079 | 0.000288 | 0.000256 | 0.006077 |
Bud Neck | 0.006879 | 0.004418 | 0.004468 | 0.005930 | 0.002661 | 0.008732 | 0.005590 | 0.007884 | 0.004184 | 0.007750 | 0.005037 | 0.006981 |
Bud Site | 0.004348 | 0.012238 | 0.010194 | 0.043371 | 0.002945 | 0.010452 | 0.006130 | 0.035024 | 0.003452 | 0.010858 | 0.008131 | 0.038882 |
Cell Periphery | 0.000172 | 0.000116 | 0.000068 | 0.000331 | 0.000069 | 0.000071 | 0.000072 | 0.000095 | 0.000106 | 0.000082 | 0.000070 | 0.000204 |
Cytoplasm | 0.599398* | 0.656581* | 0.706006* | 0.482440* | 0.687843* | 0.668544* | 0.674480* | 0.611533* | 0.655905* | 0.665823* | 0.690008* | 0.551878* |
Cytoplasmic Foci | 0.222061* | 0.122570 | 0.009646 | 0.023072 | 0.070416 | 0.096105 | 0.009692 | 0.011403 | 0.125175 | 0.102124 | 0.009670 | 0.016795 |
Eisosomes | 0.000129 | 0.000045 | 0.000012 | 0.000027 | 0.000028 | 0.000040 | 0.000020 | 0.000013 | 0.000065 | 0.000041 | 0.000016 | 0.000020 |
Endoplasmic Reticulum | 0.001375 | 0.001656 | 0.000796 | 0.001510 | 0.000829 | 0.000799 | 0.000557 | 0.000547 | 0.001026 | 0.000994 | 0.000675 | 0.000992 |
Endosome | 0.006647 | 0.009562 | 0.001461 | 0.018565 | 0.002396 | 0.006924 | 0.001189 | 0.004819 | 0.003931 | 0.007524 | 0.001323 | 0.011171 |
Golgi | 0.001850 | 0.001905 | 0.000288 | 0.017781 | 0.000672 | 0.002124 | 0.000189 | 0.007619 | 0.001097 | 0.002075 | 0.000238 | 0.012315 |
Lipid Particles | 0.005962 | 0.002949 | 0.000467 | 0.001590 | 0.001333 | 0.001636 | 0.000465 | 0.000570 | 0.003005 | 0.001935 | 0.000466 | 0.001041 |
Mitochondria | 0.001828 | 0.000531 | 0.000831 | 0.006622 | 0.000777 | 0.003876 | 0.000724 | 0.003199 | 0.001156 | 0.003115 | 0.000777 | 0.004781 |
Mitotic Spindle | 0.000079 | 0.002730 | 0.015801 | 0.144541* | 0.002096 | 0.009778 | 0.019017 | 0.055542 | 0.001367 | 0.008175 | 0.017433 | 0.096669 |
None | 0.005047 | 0.003028 | 0.005089 | 0.009607 | 0.004743 | 0.002068 | 0.006144 | 0.010789 | 0.004853 | 0.002286 | 0.005624 | 0.010243 |
Nuclear Periphery | 0.000208 | 0.000283 | 0.000879 | 0.001815 | 0.000300 | 0.000247 | 0.000557 | 0.000500 | 0.000267 | 0.000255 | 0.000716 | 0.001108 |
Nuclear Periphery Foci | 0.000512 | 0.002493 | 0.000826 | 0.004408 | 0.000731 | 0.000408 | 0.000535 | 0.001373 | 0.000652 | 0.000882 | 0.000678 | 0.002776 |
Nucleolus | 0.000548 | 0.000634 | 0.000361 | 0.000941 | 0.000490 | 0.000585 | 0.000497 | 0.000146 | 0.000511 | 0.000596 | 0.000430 | 0.000513 |
Nucleus | 0.110036 | 0.086929 | 0.173092* | 0.129949 | 0.190004* | 0.099783 | 0.184737* | 0.185361* | 0.161127* | 0.096859 | 0.179001* | 0.159755* |
Peroxisomes | 0.001517 | 0.003833 | 0.000882 | 0.001945 | 0.000922 | 0.002890 | 0.000572 | 0.004364 | 0.001137 | 0.003104 | 0.000725 | 0.003246 |
Vacuole | 0.030445 | 0.086062 | 0.067481 | 0.091061 | 0.030295 | 0.083806 | 0.088243 | 0.051998 | 0.030349 | 0.084319 | 0.078017 | 0.070050 |
Vacuole Periphery | 0.000882 | 0.000930 | 0.001022 | 0.007843 | 0.000368 | 0.000909 | 0.000408 | 0.001636 | 0.000554 | 0.000914 | 0.000711 | 0.004504 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.65 | -2.96 | -3.16 | -1.93 | -2.40 | -5.65 | -2.32 | -2.09 | -1.80 | -1.79 | -7.32 | -3.75 | -3.43 | -2.76 | -2.78 |
Bud Neck | 2.32 | 2.97 | 3.53 | 1.04 | 1.00 | -8.16 | -3.33 | -2.42 | 5.94 | 0.87 | -5.39 | -0.23 | 0.86 | 6.46 | 1.26 |
Bud Site | -4.47 | -3.18 | -5.57 | -1.12 | -3.66 | -6.44 | -2.53 | -6.21 | -0.54 | -4.83 | -7.50 | -4.50 | -8.46 | -1.51 | -5.81 |
Cell Periphery | 5.29 | 13.10 | 1.41 | -1.49 | -4.46 | -0.16 | 3.32 | -7.88 | -7.04 | -10.57 | 5.57 | 11.51 | -1.90 | -4.77 | -7.29 |
Cytoplasm | -3.79 | -2.57 | 12.14 | 13.60 | 16.61 | 2.88 | 11.80 | 19.89 | 17.01 | 9.75 | -0.79 | 7.85 | 23.80 | 23.37 | 18.61 |
Cytoplasmic Foci | 12.49 | 35.56 | 35.52 | 20.09 | 0.51 | -7.12 | 26.89 | 27.04 | 32.88 | 0.95 | 6.24 | 39.52 | 39.58 | 38.18 | 1.11 |
Eisosomes | 10.84 | 15.90 | 15.11 | 8.01 | -4.84 | -5.93 | 6.22 | 7.60 | 14.13 | 0.91 | 7.46 | 16.39 | 15.77 | 15.47 | -2.52 |
Endoplasmic Reticulum | -1.10 | 1.03 | 1.98 | 5.22 | 3.14 | 0.16 | 0.83 | 1.16 | 0.69 | 1.08 | 0.13 | 0.95 | 2.03 | 1.50 | 3.33 |
Endosome | -2.38 | 8.71 | 4.35 | 5.64 | -2.54 | -9.30 | 6.77 | 5.22 | 12.97 | -0.35 | -7.30 | 10.68 | 5.29 | 11.26 | -2.47 |
Golgi | 0.10 | 8.86 | -0.44 | -0.44 | -2.54 | -3.21 | 2.44 | -0.54 | 1.84 | -1.74 | -2.62 | 5.91 | -0.92 | 0.87 | -3.07 |
Lipid Particles | 2.55 | 8.43 | 7.07 | 1.40 | -8.36 | -0.67 | 3.33 | 0.62 | 1.22 | -9.29 | 2.46 | 8.75 | 6.07 | 1.66 | -12.23 |
Mitochondria | 3.93 | 3.59 | 1.45 | -1.97 | -1.54 | -4.11 | 1.05 | 0.05 | 4.16 | -1.11 | -3.28 | 3.15 | 0.84 | 3.61 | -1.87 |
Mitotic Spindle | -1.87 | -9.21 | -9.78 | -8.56 | -5.60 | -4.37 | -7.58 | -7.12 | -3.60 | -0.94 | -5.05 | -11.05 | -11.87 | -7.95 | -4.95 |
None | 1.60 | 0.50 | -3.32 | -4.02 | -6.02 | 4.36 | -2.10 | -7.70 | -16.42 | -6.31 | 4.72 | -1.21 | -7.73 | -14.53 | -8.94 |
Nuclear Periphery | -2.79 | -15.82 | -12.72 | -11.34 | -1.14 | 0.74 | -13.24 | -12.77 | -19.81 | 0.10 | -0.23 | -18.99 | -16.91 | -19.03 | -0.84 |
Nuclear Periphery Foci | -7.67 | -5.18 | -6.31 | 1.56 | -4.22 | 2.03 | 0.69 | -4.28 | -10.31 | -7.92 | -2.21 | -1.32 | -7.01 | -5.86 | -7.23 |
Nucleolus | -0.39 | 3.20 | 2.73 | 1.67 | 0.23 | -0.39 | 1.23 | 3.80 | 2.66 | 4.79 | -0.45 | 2.54 | 4.27 | 2.77 | 2.84 |
Nucleus | 4.08 | -20.52 | -10.97 | -14.02 | 7.53 | 18.12 | -5.60 | -6.53 | -25.30 | -1.70 | 16.89 | -14.10 | -9.45 | -27.52 | 3.49 |
Peroxisomes | -2.41 | 3.04 | 7.17 | 3.68 | 0.42 | -4.94 | 2.63 | 0.79 | 5.05 | -1.08 | -5.39 | 3.39 | 2.79 | 6.93 | -0.49 |
Vacuole | -11.96 | -23.36 | -31.54 | -18.30 | -18.45 | -16.88 | -25.63 | -25.91 | -14.95 | -7.68 | -21.04 | -34.07 | -40.06 | -24.67 | -18.56 |
Vacuole Periphery | -0.03 | 1.15 | -0.47 | -0.38 | -1.07 | -3.04 | 0.99 | 0.92 | 4.17 | 0.00 | -2.36 | 0.99 | -0.35 | 1.19 | -0.97 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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