Standard name
Human Ortholog
Description Nuclear export factor for the ribosomal pre-60S subunit; shuttling factor which directly binds FG rich nucleoporins and facilities translocation through the nuclear pore complex; interacts directly with Alb1p; responsible for Tif6p recycling defects in the absence of Rei1; associated with the ribosomal export complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0.08 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.09 0.1 0 0 – 0.07 0 0.06 0 – – – 0 0 0 0 0 0 0 0.19 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0.08 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0.07 0.05 – – – 0 0 0 0 0 0 0 0 0
Nucleus 0.93 0.89 0.94 0.64 0.87 0.66 0.86 – 0.7 0.72 0.69 0.78 – – – 0.67 0.67 0.65 0.93 0.91 0.88 0.72 0.37 0.53
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.09
Nucleolus 0 0 0.06 0.35 0.06 0.2 0.08 – 0.21 0.23 0.32 0.22 – – – 0.15 0.2 0.15 0 0 0 0.2 0.17 0.18
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.1 0 0 0 0.14 0.05 – 0.07 0.09 0.05 0 – – – 0.17 0.17 0.12 0 0.05 0.06 0.08 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 1 0 0 0 – – – 0 0 0 2 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 0 – 0 0 2 2 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 7 7 0 8 7 4 5 – 8 1 6 3 – – – 4 4 2 0 2 0 0 2 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – – – 2 1 1 1 0 0 0 0 0
Endosome 1 1 0 0 0 2 3 – 0 3 0 2 – – – 0 0 1 0 1 0 0 1 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 3 3 1 1 0 0 0 0 0
Mitochondria 0 0 0 1 0 2 1 – 2 6 8 7 – – – 3 1 2 1 0 0 0 0 0
Nucleus 238 188 15 57 62 88 163 – 83 107 74 114 – – – 92 68 42 259 206 44 4 4 13
Nuclear Periphery 0 0 0 0 0 0 2 – 5 2 1 1 – – – 0 0 0 0 0 0 0 0 2
Nucleolus 4 1 1 31 4 26 15 – 25 34 35 33 – – – 21 20 10 4 2 0 1 2 4
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 1 – – – 0 1 1 1 0 0 0 0 0
Vac/Vac Membrane 12 21 0 0 3 18 9 – 8 14 5 6 – – – 24 17 8 4 10 3 0 0 0
Unique Cell Count 255 211 16 89 71 133 189 119 149 108 147 138 102 65 280 228 51 6 13 26
Labelled Cell Count 262 218 16 97 76 141 198 132 167 131 169 149 115 68 280 228 51 6 13 26


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.2 32.3 18.5 24.9 22.1 17.0 15.4 – 11.4 11.5 11.0 11.6 – – – 49.1 47.6 41.8 – – –
Std Deviation (1e-4) 6.6 8.0 5.2 7.3 7.4 5.9 3.8 – 2.8 3.3 3.4 3.6 – – – 16.0 14.9 16.2 – – –
Intensity Change (Log2) – – – 0.43 0.26 -0.12 -0.27 – -0.69 -0.68 -0.74 -0.67 – – – 1.41 1.36 1.18 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleus -2.4 -0.7 -2.3 -0.9 – -2.0 -1.9 -2.1 -1.5 – – – -2.2 -2.2 -2.3
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.5277 27.1773 25.362 25.226 22.2344 24.341 19.3036 24.1473 21.9391 18.372 16.3568 22.0491 23.0187 26.326 22.847 22.043 23.18 23.102
Actin 0.0043 0 0 0 0.0009 0 0.004 0 0.0001 0.0004 0.0203 0 0.0078 0 0.0066 0 0 0
Bud 0.0003 0.0001 0.0001 0 0.0001 0.0001 0.0006 0 0 0.0001 0.0009 0.0001 0.0001 0 0 0.0001 0 0
Bud Neck 0.0003 0.0002 0.0001 0.0001 0.0003 0.001 0.0006 0.0001 0.0001 0.0001 0.0004 0.0005 0.0001 0.0001 0.0004 0.0001 0.0001 0.0004
Bud Periphery 0.0005 0.0002 0.0001 0 0.0002 0.0003 0.0011 0 0 0.0001 0.0014 0.0002 0.0001 0 0.0001 0 0 0.0001
Bud Site 0.0007 0.0001 0.0001 0 0.0003 0.0001 0.0008 0 0.0001 0.0002 0.0007 0 0.0001 0 0.0003 0.0001 0 0
Cell Periphery 0.001 0.0006 0.0004 0 0.0003 0.0006 0.0006 0.0001 0 0.0001 0.0009 0.0002 0.0002 0.0001 0.0001 0.0001 0 0.0001
Cytoplasm 0.0031 0.0002 0.0009 0.0001 0.0002 0.0001 0.0028 0.0001 0.0012 0.0039 0.0016 0 0.0001 0 0.0003 0.0005 0 0.0001
Cytoplasmic Foci 0.008 0 0.0002 0 0.0043 0 0.0181 0 0.0025 0.009 0.0334 0 0.0023 0 0.0029 0.023 0 0
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0 0.0003 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0011 0 0 0 0.0002 0 0.0022 0 0.0003 0.0016 0.0011 0.0001 0 0 0.0001 0 0 0
Endosome 0.0087 0 0 0 0.008 0 0.0054 0 0.0052 0.0033 0.0072 0 0.0003 0 0.0008 0.0004 0 0
Golgi 0.0046 0 0 0 0.0057 0 0.0016 0 0.0003 0.0009 0.0063 0 0.0004 0 0.0001 0.0001 0 0
Lipid Particles 0.0062 0.0001 0.0001 0 0.0061 0 0.0075 0 0.0008 0.0145 0.0196 0 0.0021 0 0.0011 0.0107 0 0
Mitochondria 0.0029 0.0003 0.0002 0.0001 0.0018 0.0002 0.0025 0.0002 0.0006 0.0006 0.0118 0.0003 0.0009 0.0005 0.0004 0.0014 0.0004 0.0003
None 0.001 0 0.0003 0 0.0001 0 0.0033 0 0.0006 0.0002 0.0021 0 0.0002 0 0.0007 0.0028 0 0
Nuclear Periphery 0.006 0.0082 0.0036 0.0029 0.0053 0.0009 0.004 0.0026 0.0039 0.0306 0.0032 0.0032 0.001 0.0016 0.0013 0.002 0.0017 0.0012
Nucleolus 0.0683 0.0576 0.0798 0.0643 0.0871 0.1174 0.1044 0.0778 0.1654 0.0722 0.129 0.1318 0.0712 0.0578 0.1056 0.0444 0.0656 0.097
Nucleus 0.8707 0.9218 0.911 0.9319 0.8716 0.8766 0.8221 0.918 0.8165 0.8409 0.7403 0.8602 0.9081 0.939 0.8769 0.9104 0.9316 0.9001
Peroxisomes 0.0019 0 0 0 0.003 0 0.007 0 0.0001 0.0003 0.0091 0 0.0003 0 0.0001 0.0003 0 0
Punctate Nuclear 0.0025 0.0002 0.0008 0.0001 0.0006 0.0001 0.006 0.0001 0.0007 0.0195 0.004 0.0001 0.0034 0.0001 0.0013 0.0018 0.0001 0.0001
Vacuole 0.005 0.0037 0.0015 0.0002 0.0021 0.0021 0.0033 0.0003 0.0009 0.0006 0.0032 0.0015 0.0006 0.0002 0.0004 0.0007 0.0001 0.0004
Vacuole Periphery 0.0029 0.0066 0.0008 0.0001 0.0019 0.0005 0.0019 0.0006 0.0006 0.0009 0.0032 0.0016 0.0006 0.0005 0.0003 0.001 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 113.3126 88.01 73.9327 64.6689 86.7038 46.4139 62.2628 41.7399 36.6746 75.4185
Translational Efficiency 1.2715 1.4491 1.4566 1.0859 1.098 1.6413 1.2578 1.1014 1.1211 1.1067

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1989 1782 799 1520 1783 2458 640 450 3772 4240 1439 1970

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1317.32 1921.45 2095.38 2634.14 1747.52 1908.02 2190.64 2742.82 1520.67 1913.66 2137.75 2658.97
Standard Deviation 296.06 452.28 355.40 696.31 359.45 402.85 401.64 656.17 391.69 424.38 379.63 688.86
Intensity Change Log 2 — 0.544590 0.669606 0.999726 — 0.126767 0.326043 0.650351 — 0.321333 0.483827 0.810979

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000486 0.000409 0.000923 0.000987 0.000507 0.000478 0.000728 0.000970 0.000496 0.000449 0.000836 0.000983
Bud Neck 0.005895 0.005625 0.022181 0.021780 0.007653 0.008813 0.013368 0.016326 0.006726 0.007473 0.018261 0.020534
Bud Site 0.000801 0.000503 0.001090 0.001918 0.000424 0.001155 0.000618 0.001185 0.000623 0.000881 0.000880 0.001751
Cell Periphery 0.000393 0.000360 0.000054 0.000149 0.000086 0.000288 0.000033 0.000091 0.000248 0.000318 0.000045 0.000136
Cytoplasm 0.004230 0.000551 0.000229 0.001773 0.000125 0.000644 0.000114 0.000648 0.002290 0.000605 0.000178 0.001516
Cytoplasmic Foci 0.000303 0.000048 0.000117 0.001342 0.000026 0.000227 0.000090 0.000911 0.000172 0.000152 0.000105 0.001243
Eisosomes 0.000067 0.000057 0.000058 0.000050 0.000075 0.000085 0.000059 0.000059 0.000070 0.000073 0.000058 0.000052
Endoplasmic Reticulum 0.003498 0.001633 0.001144 0.000429 0.001448 0.002272 0.000502 0.000258 0.002529 0.002003 0.000858 0.000390
Endosome 0.000300 0.000056 0.000035 0.000226 0.000038 0.000258 0.000015 0.001226 0.000176 0.000173 0.000026 0.000454
Golgi 0.000146 0.000040 0.000048 0.000628 0.000024 0.000065 0.000039 0.000652 0.000089 0.000054 0.000044 0.000634
Lipid Particles 0.000027 0.000010 0.000041 0.000036 0.000006 0.000117 0.000009 0.000019 0.000017 0.000072 0.000027 0.000032
Mitochondria 0.000641 0.000765 0.002671 0.004045 0.000892 0.000771 0.002184 0.003456 0.000760 0.000768 0.002454 0.003910
Mitotic Spindle 0.000027 0.000356 0.000037 0.001570 0.000007 0.001125 0.000050 0.004031 0.000018 0.000802 0.000043 0.002132
None 0.005818 0.001644 0.000511 0.001788 0.000690 0.000912 0.000542 0.001691 0.003394 0.001220 0.000525 0.001766
Nuclear Periphery 0.000112 0.000139 0.000198 0.000688 0.000096 0.000209 0.000120 0.000859 0.000104 0.000179 0.000163 0.000727
Nuclear Periphery Foci 0.000205 0.000203 0.000018 0.000196 0.000364 0.000165 0.000010 0.000262 0.000280 0.000181 0.000014 0.000211
Nucleolus 0.019004 0.007664 0.021924 0.136792 0.048703 0.011834 0.051844 0.185868 0.033043 0.010081 0.035231 0.148003
Nucleus 0.956252* 0.978937* 0.946573* 0.819686* 0.937415* 0.968679* 0.927379* 0.774089* 0.947348* 0.972990* 0.938037* 0.809271*
Peroxisomes 0.000196 0.000175 0.000982 0.002783 0.000531 0.000192 0.001653 0.004567 0.000354 0.000185 0.001280 0.003191
Vacuole 0.001355 0.000682 0.000882 0.001305 0.000556 0.001183 0.000365 0.001123 0.000977 0.000972 0.000652 0.001264
Vacuole Periphery 0.000243 0.000145 0.000285 0.001828 0.000334 0.000528 0.000278 0.001710 0.000286 0.000367 0.000282 0.001801

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.61 -4.06 -13.62 -16.03 -0.22 0.53 -7.14 -9.13 -7.60 -3.85 1.47 -5.56 -16.91 -14.60 -1.68
Bud Neck -0.15 -10.70 -15.70 -15.75 -1.58 -2.11 -7.33 -8.67 -7.91 -4.27 -2.22 -12.71 -17.67 -16.71 -4.40
Bud Site 1.41 -2.68 -4.71 -5.33 -3.53 -2.79 -4.14 -5.46 -2.96 -4.57 -1.48 -4.11 -6.52 -5.09 -5.14
Cell Periphery 0.14 5.27 3.41 1.27 -3.25 -3.62 3.31 -0.80 3.04 -2.97 -0.90 5.82 2.48 2.23 -4.13
Cytoplasm 4.55 4.97 2.26 -3.10 -3.79 -2.04 0.41 -2.60 -1.04 -2.63 3.76 4.96 0.71 -3.07 -4.35
Cytoplasmic Foci 1.36 0.91 -2.41 -3.14 -2.96 -1.46 -4.95 -3.44 -2.81 -3.24 0.13 0.49 -3.47 -3.55 -3.72
Eisosomes 1.54 0.83 2.82 1.07 2.70 -0.81 1.49 1.25 2.07 -0.15 -0.36 1.70 3.22 3.10 2.54
Endoplasmic Reticulum 8.21 7.96 15.56 9.60 3.47 -4.73 11.58 14.14 12.53 7.14 3.51 10.06 18.80 14.96 3.93
Endosome 1.60 1.67 0.46 -1.83 -1.95 -2.42 3.41 -1.04 -0.78 -1.06 -0.02 1.78 -0.94 -0.96 -1.66
Golgi 1.22 0.98 -1.05 -1.33 -1.28 -2.47 -4.21 -1.38 -1.27 -1.32 0.70 0.71 -1.51 -1.62 -1.62
Lipid Particles 1.08 -1.48 -1.10 -2.52 0.59 -1.47 -2.73 -3.06 1.15 -2.52 -1.27 -1.75 -2.27 0.72 -0.45
Mitochondria -1.87 -8.10 -19.92 -18.03 -4.00 1.87 -7.48 -9.50 -9.77 -3.97 -0.46 -10.62 -21.66 -20.93 -6.30
Mitotic Spindle -1.74 -1.34 -3.35 -2.85 -2.97 -1.92 -1.59 -2.38 -1.83 -2.35 -2.27 -1.56 -4.10 -2.89 -3.87
None 4.09 5.82 4.03 -0.37 -3.53 -1.47 1.01 -2.34 -1.86 -2.64 4.13 5.88 2.68 -1.71 -4.22
Nuclear Periphery -1.15 -3.18 -6.74 -6.24 -4.26 -2.75 -2.13 -1.99 -1.66 -1.91 -2.90 -3.55 -5.94 -5.09 -4.78
Nuclear Periphery Foci -0.24 2.15 -0.13 0.19 -2.91 0.54 1.02 0.34 -0.33 -1.35 0.38 1.50 0.32 -0.16 -3.32
Nucleolus 6.21 -1.77 -20.09* -22.53* -17.79 12.09 -1.02 -11.00 -14.25 -9.77 13.06 -1.55 -21.24 -26.32* -18.45
Nucleus -7.69 3.78 21.85 26.07 17.71 -8.76 2.42 13.06 15.56 11.06 -11.02 4.14 24.75 29.72 20.00
Peroxisomes 0.35 -6.76 -5.15 -5.17 -3.50 5.06 -5.07 -5.68 -6.12 -3.74 4.09 -7.72 -6.74 -7.10 -4.25
Vacuole 3.20 0.90 -0.74 -3.04 -1.42 -2.73 1.47 -2.13 -0.75 -2.49 -0.02 1.15 -2.36 -2.26 -3.01
Vacuole Periphery 2.38 -0.77 -4.33 -4.60 -4.03 -1.37 0.60 -3.78 -2.97 -3.91 -1.02 -0.12 -5.07 -4.62 -4.96
Standard name
Human Ortholog
Description Nuclear export factor for the ribosomal pre-60S subunit; shuttling factor which directly binds FG rich nucleoporins and facilities translocation through the nuclear pore complex; interacts directly with Alb1p; responsible for Tif6p recycling defects in the absence of Rei1; associated with the ribosomal export complex
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Arx1

Arx1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arx1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available