Standard name | |
---|---|
Human Ortholog | |
Description | Nuclear export factor for the ribosomal pre-60S subunit; shuttling factor which directly binds FG rich nucleoporins and facilities translocation through the nuclear pore complex; interacts directly with Alb1p; responsible for Tif6p recycling defects in the absence of Rei1; associated with the ribosomal export complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 2 | 2 | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 7 | 0 | 8 | 7 | 4 | 5 | – | 8 | 1 | 6 | 3 | – | – | – | 4 | 4 | 2 | 0 | 2 | 0 | 0 | 2 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1 | 1 | 0 | 0 | 0 | 2 | 3 | – | 0 | 3 | 0 | 2 | – | – | – | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 3 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 1 | 0 | 2 | 1 | – | 2 | 6 | 8 | 7 | – | – | – | 3 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 238 | 188 | 15 | 57 | 62 | 88 | 163 | – | 83 | 107 | 74 | 114 | – | – | – | 92 | 68 | 42 | 259 | 206 | 44 | 4 | 4 | 13 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 5 | 2 | 1 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 4 | 1 | 1 | 31 | 4 | 26 | 15 | – | 25 | 34 | 35 | 33 | – | – | – | 21 | 20 | 10 | 4 | 2 | 0 | 1 | 2 | 4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | – | – | – | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 12 | 21 | 0 | 0 | 3 | 18 | 9 | – | 8 | 14 | 5 | 6 | – | – | – | 24 | 17 | 8 | 4 | 10 | 3 | 0 | 0 | 0 |
Unique Cell Count | 255 | 211 | 16 | 89 | 71 | 133 | 189 | 119 | 149 | 108 | 147 | 138 | 102 | 65 | 280 | 228 | 51 | 6 | 13 | 26 | ||||
Labelled Cell Count | 262 | 218 | 16 | 97 | 76 | 141 | 198 | 132 | 167 | 131 | 169 | 149 | 115 | 68 | 280 | 228 | 51 | 6 | 13 | 26 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 23.2 | 32.3 | 18.5 | 24.9 | 22.1 | 17.0 | 15.4 | – | 11.4 | 11.5 | 11.0 | 11.6 | – | – | – | 49.1 | 47.6 | 41.8 | – | – | – |
Std Deviation (1e-4) | 6.6 | 8.0 | 5.2 | 7.3 | 7.4 | 5.9 | 3.8 | – | 2.8 | 3.3 | 3.4 | 3.6 | – | – | – | 16.0 | 14.9 | 16.2 | – | – | – |
Intensity Change (Log2) | – | – | – | 0.43 | 0.26 | -0.12 | -0.27 | – | -0.69 | -0.68 | -0.74 | -0.67 | – | – | – | 1.41 | 1.36 | 1.18 | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleus | -2.4 | -0.7 | -2.3 | -0.9 | – | -2.0 | -1.9 | -2.1 | -1.5 | – | – | – | -2.2 | -2.2 | -2.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 23.5277 | 27.1773 | 25.362 | 25.226 | 22.2344 | 24.341 | 19.3036 | 24.1473 | 21.9391 | 18.372 | 16.3568 | 22.0491 | 23.0187 | 26.326 | 22.847 | 22.043 | 23.18 | 23.102 |
Actin | 0.0043 | 0 | 0 | 0 | 0.0009 | 0 | 0.004 | 0 | 0.0001 | 0.0004 | 0.0203 | 0 | 0.0078 | 0 | 0.0066 | 0 | 0 | 0 |
Bud | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0006 | 0 | 0 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Bud Neck | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.001 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0004 |
Bud Periphery | 0.0005 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0003 | 0.0011 | 0 | 0 | 0.0001 | 0.0014 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Bud Site | 0.0007 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0008 | 0 | 0.0001 | 0.0002 | 0.0007 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0 | 0 |
Cell Periphery | 0.001 | 0.0006 | 0.0004 | 0 | 0.0003 | 0.0006 | 0.0006 | 0.0001 | 0 | 0.0001 | 0.0009 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.0031 | 0.0002 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0028 | 0.0001 | 0.0012 | 0.0039 | 0.0016 | 0 | 0.0001 | 0 | 0.0003 | 0.0005 | 0 | 0.0001 |
Cytoplasmic Foci | 0.008 | 0 | 0.0002 | 0 | 0.0043 | 0 | 0.0181 | 0 | 0.0025 | 0.009 | 0.0334 | 0 | 0.0023 | 0 | 0.0029 | 0.023 | 0 | 0 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0011 | 0 | 0 | 0 | 0.0002 | 0 | 0.0022 | 0 | 0.0003 | 0.0016 | 0.0011 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endosome | 0.0087 | 0 | 0 | 0 | 0.008 | 0 | 0.0054 | 0 | 0.0052 | 0.0033 | 0.0072 | 0 | 0.0003 | 0 | 0.0008 | 0.0004 | 0 | 0 |
Golgi | 0.0046 | 0 | 0 | 0 | 0.0057 | 0 | 0.0016 | 0 | 0.0003 | 0.0009 | 0.0063 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Lipid Particles | 0.0062 | 0.0001 | 0.0001 | 0 | 0.0061 | 0 | 0.0075 | 0 | 0.0008 | 0.0145 | 0.0196 | 0 | 0.0021 | 0 | 0.0011 | 0.0107 | 0 | 0 |
Mitochondria | 0.0029 | 0.0003 | 0.0002 | 0.0001 | 0.0018 | 0.0002 | 0.0025 | 0.0002 | 0.0006 | 0.0006 | 0.0118 | 0.0003 | 0.0009 | 0.0005 | 0.0004 | 0.0014 | 0.0004 | 0.0003 |
None | 0.001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0033 | 0 | 0.0006 | 0.0002 | 0.0021 | 0 | 0.0002 | 0 | 0.0007 | 0.0028 | 0 | 0 |
Nuclear Periphery | 0.006 | 0.0082 | 0.0036 | 0.0029 | 0.0053 | 0.0009 | 0.004 | 0.0026 | 0.0039 | 0.0306 | 0.0032 | 0.0032 | 0.001 | 0.0016 | 0.0013 | 0.002 | 0.0017 | 0.0012 |
Nucleolus | 0.0683 | 0.0576 | 0.0798 | 0.0643 | 0.0871 | 0.1174 | 0.1044 | 0.0778 | 0.1654 | 0.0722 | 0.129 | 0.1318 | 0.0712 | 0.0578 | 0.1056 | 0.0444 | 0.0656 | 0.097 |
Nucleus | 0.8707 | 0.9218 | 0.911 | 0.9319 | 0.8716 | 0.8766 | 0.8221 | 0.918 | 0.8165 | 0.8409 | 0.7403 | 0.8602 | 0.9081 | 0.939 | 0.8769 | 0.9104 | 0.9316 | 0.9001 |
Peroxisomes | 0.0019 | 0 | 0 | 0 | 0.003 | 0 | 0.007 | 0 | 0.0001 | 0.0003 | 0.0091 | 0 | 0.0003 | 0 | 0.0001 | 0.0003 | 0 | 0 |
Punctate Nuclear | 0.0025 | 0.0002 | 0.0008 | 0.0001 | 0.0006 | 0.0001 | 0.006 | 0.0001 | 0.0007 | 0.0195 | 0.004 | 0.0001 | 0.0034 | 0.0001 | 0.0013 | 0.0018 | 0.0001 | 0.0001 |
Vacuole | 0.005 | 0.0037 | 0.0015 | 0.0002 | 0.0021 | 0.0021 | 0.0033 | 0.0003 | 0.0009 | 0.0006 | 0.0032 | 0.0015 | 0.0006 | 0.0002 | 0.0004 | 0.0007 | 0.0001 | 0.0004 |
Vacuole Periphery | 0.0029 | 0.0066 | 0.0008 | 0.0001 | 0.0019 | 0.0005 | 0.0019 | 0.0006 | 0.0006 | 0.0009 | 0.0032 | 0.0016 | 0.0006 | 0.0005 | 0.0003 | 0.001 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 113.3126 | 88.01 | 73.9327 | 64.6689 | 86.7038 | 46.4139 | 62.2628 | 41.7399 | 36.6746 | 75.4185 |
Translational Efficiency | 1.2715 | 1.4491 | 1.4566 | 1.0859 | 1.098 | 1.6413 | 1.2578 | 1.1014 | 1.1211 | 1.1067 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|