Standard name | |
---|---|
Human Ortholog | |
Description | Component of transport protein particle (TRAPP) complex III; TRAPPIII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating endosome-Golgi traffic and required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 11 | 2 | 2 | 10 | 11 | 1 | 11 | 9 | 4 | 3 | 1 | 7 | 0 | 0 | 0 | 1 | 1 | 0 | 9 | 1 | 3 | 5 | 10 | 8 |
Bud | 2 | 5 | 2 | 1 | 8 | 11 | 17 | 10 | 25 | 25 | 33 | 17 | 3 | 6 | 4 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 5 | 5 |
Bud Neck | 0 | 0 | 3 | 1 | 6 | 3 | 2 | 3 | 2 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 2 | 8 | 6 | 8 | 9 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 27 | 15 | 11 | 7 | 5 | 7 | 4 | 8 | 7 | 8 | 4 | 6 | 6 | 7 | 2 | 0 | 0 | 0 | 1 | 1 | 2 | 3 |
Cytoplasm | 249 | 246 | 371 | 316 | 441 | 441 | 575 | 487 | 376 | 403 | 302 | 302 | 421 | 561 | 614 | 75 | 53 | 15 | 209 | 221 | 319 | 132 | 214 | 236 |
Endoplasmic Reticulum | 9 | 7 | 14 | 4 | 11 | 7 | 10 | 13 | 5 | 2 | 2 | 6 | 5 | 3 | 4 | 5 | 2 | 0 | 27 | 4 | 8 | 26 | 66 | 44 |
Endosome | 9 | 0 | 0 | 1 | 0 | 5 | 5 | 7 | 4 | 3 | 0 | 8 | 1 | 3 | 2 | 5 | 0 | 0 | 17 | 3 | 4 | 3 | 5 | 4 |
Golgi | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 7 | 0 | 1 | 2 | 4 | 0 |
Mitochondria | 22 | 20 | 0 | 14 | 25 | 133 | 160 | 83 | 238 | 245 | 271 | 129 | 8 | 6 | 2 | 1 | 0 | 0 | 23 | 27 | 86 | 42 | 66 | 51 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 7 | 5 | 4 | 4 | 8 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 1 | 0 | 0 | 2 | 1 | 2 | 8 | 13 | 2 | 1 | 1 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 2 | 1 |
Vac/Vac Membrane | 1 | 3 | 0 | 5 | 3 | 19 | 8 | 25 | 6 | 10 | 31 | 11 | 2 | 1 | 3 | 4 | 1 | 4 | 13 | 23 | 19 | 24 | 19 | 25 |
Unique Cell Count | 288 | 265 | 395 | 342 | 488 | 556 | 722 | 608 | 544 | 577 | 524 | 447 | 432 | 572 | 625 | 89 | 56 | 18 | 330 | 294 | 462 | 251 | 414 | 399 |
Labelled Cell Count | 308 | 283 | 419 | 369 | 518 | 629 | 807 | 666 | 673 | 707 | 657 | 508 | 444 | 588 | 635 | 100 | 60 | 20 | 330 | 294 | 462 | 251 | 414 | 399 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 5.1 | 6.1 | 5.6 | 6.0 | 4.7 | 5.0 | 5.3 | 4.3 | 4.2 | 4.1 | 4.8 | 5.9 | 5.9 | 6.1 | 9.5 | 9.4 | 10.1 | 5.3 | 5.6 | 5.8 |
Std Deviation (1e-4) | 0.7 | 0.8 | 0.6 | 0.8 | 1.2 | 0.7 | 0.9 | 0.7 | 0.7 | 0.7 | 0.8 | 0.8 | 0.9 | 0.8 | 1.0 | 1.9 | 2.0 | 1.7 | 0.9 | 1.4 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.02 | -0.38 | -0.29 | -0.22 | -0.5 | -0.53 | -0.58 | -0.33 | -0.05 | -0.05 | 0.0 | 0.63 | 0.63 | 0.73 | -0.19 | -0.11 | -0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.6 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.4 | -3.3 | 0 | -5.9 | -4.9 | 0 | 0 | 0 | 0 | -4.5 | -4.9 | -5.2 | 0.3 | 0 | 0 |
Cytoplasm | -0.8 | -1.9 | -6.3 | -6.3 | -6.1 | -9.3 | -9.2 | -12.3 | -9.5 | 2.5 | 3.4 | 3.7 | -3.1 | 0.2 | -1.8 |
Endoplasmic Reticulum | -2.1 | -1.1 | 0 | -2.4 | -1.3 | 0 | 0 | 0 | 0 | -2.3 | -3.5 | -3.4 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.1 | 4.6 | 0 | 10.1 | 7.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 3.7 | 0 | 4.1 | 0 | 0 | 4.9 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.6082 | 0.8923 | 0.6501 | 0.4392 | 0.6353 | 0.8907 | 0.9901 | 1.4706 | 1.3807 | 1.2214 | 0.6736 | 1.2836 | 0.4729 | 1.0277 | 0.8078 | 0.5531 | 0.3469 | 0.8291 |
Actin | 0.0225 | 0.0025 | 0.025 | 0.0031 | 0.0186 | 0.0099 | 0.0258 | 0.0021 | 0.002 | 0.0044 | 0.0097 | 0.0038 | 0.0168 | 0.0013 | 0.0136 | 0.0004 | 0.0321 | 0.004 |
Bud | 0.0013 | 0.0015 | 0.0028 | 0.0086 | 0.0006 | 0.0004 | 0.0012 | 0.0007 | 0.0032 | 0.0017 | 0.0022 | 0.0018 | 0.0008 | 0.0004 | 0.0013 | 0.0003 | 0.0016 | 0.0003 |
Bud Neck | 0.0131 | 0.0011 | 0.0018 | 0.001 | 0.0006 | 0.0036 | 0.002 | 0.0004 | 0.0005 | 0.0019 | 0.0013 | 0.0022 | 0.0064 | 0.0003 | 0.0018 | 0.0014 | 0.0009 | 0.0039 |
Bud Periphery | 0.0019 | 0.0013 | 0.0027 | 0.0031 | 0.0006 | 0.0007 | 0.002 | 0.0003 | 0.0025 | 0.0009 | 0.0024 | 0.0014 | 0.0011 | 0.0002 | 0.0013 | 0.0002 | 0.0023 | 0.0003 |
Bud Site | 0.021 | 0.015 | 0.0119 | 0.003 | 0.0008 | 0.0006 | 0.0057 | 0.0144 | 0.0043 | 0.0014 | 0.0114 | 0.0004 | 0.0102 | 0.0036 | 0.0072 | 0.0004 | 0.0023 | 0.0003 |
Cell Periphery | 0.0015 | 0.0009 | 0.0016 | 0.0007 | 0.0002 | 0.0003 | 0.0009 | 0.0004 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0016 | 0.0003 | 0.0006 | 0.0002 | 0.0004 | 0.0002 |
Cytoplasm | 0.5696 | 0.6809 | 0.5766 | 0.5916 | 0.516 | 0.6904 | 0.6471 | 0.8094 | 0.7966 | 0.7833 | 0.739 | 0.7821 | 0.6725 | 0.7806 | 0.7459 | 0.7839 | 0.5534 | 0.8035 |
Cytoplasmic Foci | 0.0537 | 0.0439 | 0.0695 | 0.0438 | 0.0531 | 0.0455 | 0.0649 | 0.0489 | 0.0333 | 0.0573 | 0.0545 | 0.0489 | 0.0675 | 0.0442 | 0.0487 | 0.0277 | 0.0923 | 0.0392 |
Eisosomes | 0.0005 | 0.0002 | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0004 | 0 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0135 | 0.0065 | 0.0123 | 0.0067 | 0.0086 | 0.0121 | 0.0063 | 0.0032 | 0.0032 | 0.0032 | 0.0044 | 0.0047 | 0.018 | 0.0042 | 0.0087 | 0.0062 | 0.0093 | 0.0076 |
Endosome | 0.0649 | 0.0465 | 0.1138 | 0.0936 | 0.0763 | 0.0754 | 0.0792 | 0.0423 | 0.0525 | 0.0655 | 0.0849 | 0.062 | 0.081 | 0.0567 | 0.0867 | 0.1434 | 0.1115 | 0.0681 |
Golgi | 0.0081 | 0.0053 | 0.015 | 0.0043 | 0.0101 | 0.0094 | 0.0201 | 0.0042 | 0.0062 | 0.0249 | 0.0133 | 0.0058 | 0.0147 | 0.0068 | 0.0103 | 0.0027 | 0.0176 | 0.0064 |
Lipid Particles | 0.0072 | 0.0007 | 0.0057 | 0.0046 | 0.0023 | 0.0054 | 0.0143 | 0.0006 | 0.0028 | 0.0005 | 0.0026 | 0.0062 | 0.0087 | 0.001 | 0.0016 | 0.0003 | 0.0039 | 0.0006 |
Mitochondria | 0.012 | 0.0025 | 0.0141 | 0.0087 | 0.0061 | 0.0059 | 0.0083 | 0.0011 | 0.002 | 0.0047 | 0.0063 | 0.0058 | 0.0137 | 0.0016 | 0.0044 | 0.0006 | 0.0247 | 0.0026 |
None | 0.1681 | 0.1753 | 0.1211 | 0.1783 | 0.2691 | 0.1178 | 0.091 | 0.0642 | 0.0757 | 0.0371 | 0.0368 | 0.064 | 0.0683 | 0.0908 | 0.0499 | 0.0127 | 0.1145 | 0.0538 |
Nuclear Periphery | 0.0118 | 0.0017 | 0.005 | 0.0106 | 0.0215 | 0.0058 | 0.0054 | 0.0006 | 0.0006 | 0.001 | 0.0034 | 0.0006 | 0.0018 | 0.0007 | 0.0017 | 0.001 | 0.0021 | 0.0029 |
Nucleolus | 0.0009 | 0.0002 | 0.0003 | 0.0058 | 0.0005 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 |
Nucleus | 0.0049 | 0.0023 | 0.0023 | 0.0074 | 0.0034 | 0.0016 | 0.0019 | 0.001 | 0.001 | 0.001 | 0.0015 | 0.0008 | 0.0011 | 0.0009 | 0.0015 | 0.0008 | 0.0013 | 0.001 |
Peroxisomes | 0.0068 | 0.0007 | 0.0016 | 0.0009 | 0.0006 | 0.0057 | 0.0133 | 0.0012 | 0.0039 | 0.0016 | 0.0185 | 0.0033 | 0.0042 | 0.0003 | 0.0014 | 0.0001 | 0.0123 | 0.0008 |
Punctate Nuclear | 0.0081 | 0.0011 | 0.006 | 0.0028 | 0.0026 | 0.0017 | 0.0013 | 0.0006 | 0.0004 | 0.0008 | 0.0017 | 0.0005 | 0.0021 | 0.0004 | 0.0032 | 0.0001 | 0.0009 | 0.0007 |
Vacuole | 0.0069 | 0.0087 | 0.0079 | 0.0165 | 0.005 | 0.0055 | 0.0076 | 0.0037 | 0.0076 | 0.0068 | 0.0043 | 0.004 | 0.0071 | 0.0046 | 0.0079 | 0.0132 | 0.0129 | 0.0023 |
Vacuole Periphery | 0.0018 | 0.0013 | 0.0021 | 0.0046 | 0.0031 | 0.0017 | 0.0012 | 0.0007 | 0.0013 | 0.0017 | 0.0013 | 0.0013 | 0.0013 | 0.0008 | 0.0017 | 0.0043 | 0.0029 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.576 | 7.0556 | 6.9808 | 9.6673 | 12.621 | 9.466 | 11.8899 | 11.2348 | 12.2316 | 12.8962 |
Translational Efficiency | 0.5916 | 0.6379 | 0.7309 | 0.453 | 0.4248 | 0.5528 | 0.5136 | 0.3892 | 0.4285 | 0.4791 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1960 | 601 | 265 | 1152 | 91 | 1248 | 1347 | 2006 | 2051 | 1849 | 1612 | 3158 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 752.54 | 920.52 | 1125.10 | 954.58 | 900.23 | 845.66 | 979.40 | 946.50 | 759.09 | 869.99 | 1003.35 | 949.45 |
Standard Deviation | 80.76 | 127.13 | 159.09 | 108.81 | 92.79 | 116.25 | 117.14 | 125.69 | 86.83 | 124.92 | 136.17 | 119.87 |
Intensity Change Log 2 | — | 0.290681 | 0.580213 | 0.343098 | — | -0.090216 | 0.121605 | 0.072309 | — | 0.095746 | 0.348592 | 0.201933 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000278 | 0.001437 | 0.001933 | 0.002926 | 0.000740 | 0.001093 | 0.000772 | 0.000910 | 0.000299 | 0.001205 | 0.000963 | 0.001645 |
Bud Neck | 0.015012 | 0.011360 | 0.004534 | 0.008837 | 0.005857 | 0.013805 | 0.005040 | 0.010927 | 0.014606 | 0.013010 | 0.004957 | 0.010165 |
Bud Site | 0.019294 | 0.040885 | 0.025851 | 0.047349 | 0.017178 | 0.037902 | 0.017732 | 0.037876 | 0.019200 | 0.038872 | 0.019067 | 0.041332 |
Cell Periphery | 0.000280 | 0.000184 | 0.000352 | 0.000127 | 0.000444 | 0.000188 | 0.000096 | 0.000066 | 0.000287 | 0.000187 | 0.000138 | 0.000088 |
Cytoplasm | 0.389993* | 0.342324* | 0.528756* | 0.347444* | 0.323642* | 0.274996* | 0.598442* | 0.431248* | 0.387049* | 0.296880* | 0.586986* | 0.400678* |
Cytoplasmic Foci | 0.376277* | 0.403484* | 0.095654 | 0.184650* | 0.401423* | 0.427373* | 0.118475 | 0.135868 | 0.377393* | 0.419608* | 0.114724 | 0.153663 |
Eisosomes | 0.000271 | 0.000163 | 0.000052 | 0.000077 | 0.000255 | 0.000218 | 0.000039 | 0.000035 | 0.000270 | 0.000200 | 0.000041 | 0.000050 |
Endoplasmic Reticulum | 0.003946 | 0.001806 | 0.005211 | 0.001436 | 0.003482 | 0.001818 | 0.002106 | 0.000759 | 0.003925 | 0.001814 | 0.002617 | 0.001006 |
Endosome | 0.040048 | 0.058011 | 0.020112 | 0.052771 | 0.087748 | 0.080618 | 0.014935 | 0.042123 | 0.042165 | 0.073270 | 0.015786 | 0.046007 |
Golgi | 0.040571 | 0.038175 | 0.001231 | 0.006685 | 0.042049 | 0.061467 | 0.000945 | 0.005021 | 0.040637 | 0.053896 | 0.000992 | 0.005628 |
Lipid Particles | 0.012130 | 0.003305 | 0.005290 | 0.003451 | 0.012948 | 0.006574 | 0.003309 | 0.002412 | 0.012167 | 0.005512 | 0.003635 | 0.002791 |
Mitochondria | 0.014248 | 0.004290 | 0.000154 | 0.001639 | 0.001450 | 0.013296 | 0.000325 | 0.002305 | 0.013680 | 0.010369 | 0.000297 | 0.002062 |
Mitotic Spindle | 0.003548 | 0.005814 | 0.014771 | 0.015210 | 0.000404 | 0.005291 | 0.011378 | 0.050719 | 0.003409 | 0.005461 | 0.011936 | 0.037766 |
None | 0.002773 | 0.000339 | 0.000667 | 0.000259 | 0.000313 | 0.000201 | 0.001412 | 0.001078 | 0.002664 | 0.000246 | 0.001289 | 0.000779 |
Nuclear Periphery | 0.000114 | 0.000063 | 0.000913 | 0.000346 | 0.000265 | 0.000068 | 0.000580 | 0.000537 | 0.000120 | 0.000066 | 0.000635 | 0.000467 |
Nuclear Periphery Foci | 0.000264 | 0.000289 | 0.019568 | 0.002721 | 0.001028 | 0.000288 | 0.003353 | 0.001390 | 0.000297 | 0.000288 | 0.006019 | 0.001876 |
Nucleolus | 0.000601 | 0.000156 | 0.000176 | 0.000323 | 0.000904 | 0.000284 | 0.000305 | 0.000666 | 0.000614 | 0.000243 | 0.000283 | 0.000541 |
Nucleus | 0.013989 | 0.004739 | 0.064177 | 0.011711 | 0.009320 | 0.004354 | 0.047419 | 0.015062 | 0.013782 | 0.004479 | 0.050174 | 0.013839 |
Peroxisomes | 0.004174 | 0.007740 | 0.001138 | 0.012007 | 0.005154 | 0.010618 | 0.002662 | 0.008672 | 0.004218 | 0.009682 | 0.002412 | 0.009889 |
Vacuole | 0.052413 | 0.067859 | 0.208621* | 0.297394* | 0.072963 | 0.050835 | 0.169895 | 0.248239* | 0.053325 | 0.056368 | 0.176261 | 0.266170* |
Vacuole Periphery | 0.009777 | 0.007576 | 0.000840 | 0.002637 | 0.012431 | 0.008713 | 0.000779 | 0.004088 | 0.009894 | 0.008343 | 0.000789 | 0.003559 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.12 | -6.30 | -5.32 | -1.88 | -0.57 | -1.55 | 0.03 | 0.24 | 2.51 | 0.34 | -7.17 | -6.96 | -6.24 | -0.40 | -1.85 |
Bud Neck | 2.92 | 11.18 | 6.98 | 2.87 | -2.32 | -6.69 | 0.94 | -4.02 | 3.48 | -6.33 | 1.66 | 11.90 | 6.39 | 4.59 | -6.32 |
Bud Site | -6.70 | -2.39 | -6.13 | 0.85 | -3.29 | -5.27 | 0.56 | -2.73 | 3.72 | -5.19 | -10.00 | 1.36 | -6.31 | 3.36 | -6.96 |
Cell Periphery | 3.10 | -2.44 | 9.10 | 2.81 | 8.17 | 4.22 | 6.06 | 6.71 | 7.94 | 6.26 | 4.56 | 8.23 | 12.41 | 7.83 | 8.10 |
Cytoplasm | 3.73 | -5.08 | 3.90 | -0.45 | 6.95 | 1.88 | -9.08 | -3.69 | -14.70 | 13.47 | 11.18 | -17.86 | -0.90 | -12.01 | 17.05 |
Cytoplasmic Foci | -2.20 | 39.71 | 32.48 | 22.62 | -9.63 | -1.18 | 13.50 | 13.14 | 44.07 | -1.61 | -5.92 | 49.03 | 45.93 | 48.48 | -6.19 |
Eisosomes | 3.38 | 14.43 | 13.48 | 3.28 | -0.86 | 0.92 | 5.80 | 6.04 | 11.40 | 3.16 | 3.53 | 16.37 | 16.23 | 10.64 | 0.02 |
Endoplasmic Reticulum | 3.36 | -2.78 | 6.14 | 1.28 | 8.44 | 1.55 | 1.52 | 2.99 | 2.50 | 7.85 | 3.93 | 2.97 | 7.61 | 2.75 | 9.91 |
Endosome | -4.35 | 9.50 | 0.63 | 4.20 | -6.17 | 0.57 | 6.25 | 4.58 | 14.09 | -10.97 | -10.94 | 16.90 | 3.78 | 13.93 | -13.02 |
Golgi | 0.96 | 26.69 | 20.79 | 11.26 | -3.56 | -2.24 | 4.96 | 4.66 | 21.98 | -5.10 | -5.23 | 27.33 | 24.29 | 24.44 | -6.18 |
Lipid Particles | 12.24 | 8.40 | 12.78 | 0.32 | 5.60 | 2.20 | 3.50 | 3.78 | 5.69 | 3.05 | 8.17 | 12.77 | 14.50 | 5.52 | 4.03 |
Mitochondria | 6.99 | 12.76 | 11.48 | 3.24 | -2.85 | -7.37 | 4.04 | -0.47 | 7.30 | -5.66 | 2.19 | 12.69 | 11.45 | 7.90 | -6.05 |
Mitotic Spindle | -1.07 | -3.24 | -4.42 | -2.89 | 0.51 | -4.27 | -7.32 | -13.54 | -11.24 | -8.05 | -1.60 | -5.82 | -12.50 | -11.36 | -6.73 |
None | 4.09 | 3.69 | 4.20 | 0.27 | 2.83 | 0.87 | -2.09 | -2.72 | -3.40 | 0.50 | 4.30 | 2.40 | 3.38 | -3.14 | 1.02 |
Nuclear Periphery | 2.82 | -10.73 | -6.32 | -7.49 | 7.42 | 1.89 | -3.97 | -4.19 | -12.48 | 0.04 | 3.03 | -11.88 | -12.79 | -14.38 | 3.03 |
Nuclear Periphery Foci | -0.69 | -12.10 | -4.96 | -4.87 | 10.30 | 3.31 | -6.78 | -0.99 | -4.72 | 6.02 | 0.00 | -14.92 | -6.86 | -6.74 | 9.90 |
Nucleolus | 2.90 | 2.70 | 1.94 | -3.41 | -2.89 | 0.84 | 0.76 | 0.12 | -3.93 | -3.94 | 1.99 | 1.47 | -0.08 | -4.26 | -3.20 |
Nucleus | 11.05 | -9.49 | -1.00 | -9.61 | 9.29 | 2.97 | -18.58 | -6.14 | -12.88 | 16.55 | 11.40 | -21.74 | -5.16 | -15.53 | 19.57 |
Peroxisomes | -4.76 | 8.56 | -5.05 | -1.35 | -8.63 | -4.44 | 2.75 | -1.61 | 4.00 | -7.72 | -8.30 | 5.18 | -6.09 | 2.63 | -10.60 |
Vacuole | -4.05 | -15.02 | -34.93 | -29.55 | -5.90 | 1.87 | -10.42 | -17.40 | -39.70 | -11.31 | -1.64 | -29.41 | -51.30 | -49.62 | -14.29 |
Vacuole Periphery | 2.27 | 16.94 | 12.00 | 5.93 | -2.46 | 0.71 | 2.30 | 1.90 | 8.63 | -4.43 | 2.22 | 16.65 | 11.86 | 10.63 | -5.11 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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