Standard name
Human Ortholog
Description Protein of unknown function; proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.08 0.07 0.06 0.13 0.09 0.18 0.15 0 0.07 0 0.09 0.11 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.06 0.05 0.07 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.05 0.12 0 0 0 0 0 0 0 0.05 0 0 0 0 0.08 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.18 0.12 0.3 0.3 0.35 0.45 0.41 0.44 0.45 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.09 0.1 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.07 0.09 0.08 0.05 0.05 0 0.08 0.07 0.09
Nuclear Periphery 0.09 0.1 0.29 0.3 0.15 0.24 0.27 0.16 0.13 0.17 0.09 0.09 0.19 0.19 0.2 0.33 0.37 0.3 0.05 0.05 0.16 0.08 0.07 0.06
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.82 0.83 0.83 0.82 0.79 0.54 0.57 0.53 0.44 0.47 0.41 0.44 0.9 0.84 0.9 0.78 0.71 0.77 0.84 0.86 0.77 0.75 0.76 0.72
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 1 1 5 3 1 1 2 0 0 0 0 0 0 0 0 0 0 2 1
Bud 0 0 0 0 0 0 0 2 1 0 1 0 0 0 0 0 0 0 1 3 2 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 3 6 20 18 20 17 11 14 1 0 0 2 1 9 0 0 0 0 0 0
Cytoplasm 12 17 17 8 18 35 40 38 64 50 54 62 2 9 8 37 33 23 0 2 0 0 0 0
Endoplasmic Reticulum 0 1 1 0 3 16 16 38 24 39 24 23 0 0 0 8 6 5 0 0 1 0 1 0
Endosome 0 2 2 3 1 1 1 1 2 0 1 0 0 0 0 0 1 0 1 1 1 2 3 5
Golgi 12 3 8 15 48 13 12 21 11 10 3 2 5 1 6 4 2 18 1 0 1 0 2 0
Mitochondria 3 12 18 50 50 131 166 215 225 226 131 183 1 0 0 19 13 4 2 5 13 3 3 4
Nucleus 26 37 4 1 12 15 5 7 7 8 4 3 3 9 9 30 28 18 15 20 2 10 17 17
Nuclear Periphery 25 40 108 86 61 105 145 95 66 94 26 36 19 26 35 140 112 70 15 21 61 10 17 11
Nucleolus 10 5 14 7 9 9 6 5 5 0 2 2 0 1 0 6 7 4 5 3 4 4 8 7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 1 0
Vac/Vac Membrane 225 317 314 231 320 235 311 325 220 258 124 180 91 114 161 325 216 182 240 355 297 103 198 133
Unique Cell Count 274 383 378 282 407 433 546 608 502 551 299 405 101 136 179 418 303 235 286 414 388 137 262 186
Labelled Cell Count 313 435 487 402 525 567 723 770 648 703 382 507 122 160 219 572 419 334 286 414 388 137 262 186


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 22.4 22.9 22.3 19.5 16.2 13.0 11.5 11.3 10.6 9.5 9.6 9.4 18.3 16.7 15.4 12.0 12.3 16.5 18.5 19.4 20.7
Std Deviation (1e-4) 5.9 6.2 5.3 4.1 3.9 3.6 3.2 2.8 2.6 2.5 2.5 2.3 4.6 4.3 3.6 3.5 4.5 5.6 5.4 5.9 6.6
Intensity Change (Log2) – – – -0.2 -0.46 -0.78 -0.95 -0.98 -1.08 -1.23 -1.23 -1.25 -0.29 -0.42 -0.54 -0.89 -0.86 -0.44 -0.27 -0.2 -0.11

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70001020WT3HU80HU120HU16001020WT3rpd3Δ_1rpd3Δ_2rpd3Δ_301020WT1AF100AF140AF18001020
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3.4 3.0 3.6 3.1 3.3 3.3 0 0 0 0 0 0
Cytoplasm -1.1 -0.1 2.1 1.8 1.2 4.2 2.6 5.7 5.0 0 1.0 0 2.4 3.2 2.6
Endoplasmic Reticulum 0 0 3.4 3.0 4.7 4.0 5.0 5.3 4.4 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 2.2 5.3 0.8 0.1 1.2 0.1 -0.3 0 0 0 0 0 -1.3 0 3.3
Mitochondria 5.4 3.7 9.4 9.6 11.0 13.2 12.3 12.2 12.9 0 0 -3.0 -0.1 -0.3 -2.0
Nucleus 0 1.9 2.3 0 0 0 0 0 0 0 0 0 4.3 5.0 4.3
Nuclear Periphery 0.5 -4.6 -1.4 -0.7 -4.9 -5.7 -4.2 -6.4 -7.1 -2.0 -2.2 -2.3 1.5 2.3 0.3
Nucleolus -0.9 -1.2 -1.4 -2.7 -3.2 -2.7 -4.6 -2.6 -3.2 0 0 0 -2.0 -1.0 -1.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.4 -1.6 -8.7 -8.3 -9.5 -11.8 -11.2 -11.2 -11.2 1.7 0.2 2.1 -1.9 -3.7 -1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.5503 14.1061 13.8415 10.8621 10.4671 12.0007 13.2945 15.158 14.0135 13.0574 13.4781 13.5814 15.6927 17.2807 16.8459 16.7756 15.041 16.0554
Actin 0.001 0 0.0044 0.0033 0.0033 0 0.0002 0 0.0018 0.0161 0 0 0.0037 0 0.0004 0 0 0
Bud 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0003 0.0007 0 0 0.0001 0 0.0001 0 0 0
Bud Neck 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0.0002 0.0001 0 0 0.0001 0 0 0 0 0
Bud Periphery 0.0002 0 0.0004 0.0002 0.0006 0.0002 0.0002 0.0001 0.0011 0.0029 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001
Bud Site 0.0004 0 0.0002 0.0001 0.0001 0 0.0002 0 0.0003 0.0004 0 0 0.0001 0 0 0 0 0
Cell Periphery 0.0009 0.0003 0.0007 0.0003 0.0007 0.0003 0.0006 0.0006 0.0008 0.0003 0.0002 0.0002 0.0005 0.0004 0.0005 0.0002 0.0002 0.0002
Cytoplasm 0.0014 0.0001 0.0001 0 0.0004 0.0001 0.0001 0 0.0002 0.0001 0 0 0.0001 0.0001 0.0001 0 0 0
Cytoplasmic Foci 0.0018 0 0.0005 0.0006 0.0004 0.0001 0.004 0 0.0017 0.0002 0 0 0.0001 0 0.0009 0 0.0001 0
Eisosomes 0 0 0 0.0001 0 0 0 0 0 0.0002 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0011 0.0001 0.0001 0.0001 0.0015 0.0001 0.0003 0.0003 0.0006 0.0017 0.0001 0.0002 0.0002 0.0003 0.0002 0 0.0001 0.0001
Endosome 0.0055 0.0018 0.003 0.0021 0.0073 0.0038 0.0081 0.0029 0.009 0.0062 0.0012 0.0035 0.0033 0.0016 0.0041 0.0016 0.0009 0.0017
Golgi 0.0004 0 0.0004 0.0006 0.0007 0.0001 0.0011 0 0.0016 0.0036 0 0.0001 0.0003 0 0.0004 0 0 0
Lipid Particles 0.0038 0.0001 0.0009 0.0035 0.0018 0.0005 0.004 0.0001 0.0024 0.0016 0.0002 0.0006 0.0006 0.0001 0.0009 0.0001 0.0006 0.0002
Mitochondria 0.005 0.0002 0.0038 0.0236 0.0025 0.0018 0.0159 0.0009 0.0137 0.0036 0.018 0.0006 0.0054 0.0002 0.009 0.0002 0.0002 0.0003
None 0.0008 0 0.0001 0.0001 0.0006 0 0.0001 0 0.0004 0.0002 0 0 0.0001 0 0 0 0 0
Nuclear Periphery 0.0202 0.0175 0.0084 0.003 0.0266 0.0115 0.0136 0.0125 0.0132 0.0037 0.0024 0.009 0.0061 0.0078 0.0111 0.0007 0.0029 0.0093
Nucleolus 0.0025 0.0001 0.0004 0.0001 0.0018 0.0003 0.0009 0.0001 0.0004 0.0001 0.0001 0.0002 0.0006 0.0001 0.0002 0.0001 0.0192 0.0002
Nucleus 0.0043 0.0008 0.0005 0.0002 0.0042 0.0006 0.003 0.0004 0.001 0.0004 0.0002 0.0003 0.0007 0.0003 0.0004 0.0002 0.0051 0.0002
Peroxisomes 0.0002 0 0.0005 0.0018 0.0001 0 0.0012 0 0.003 0.0002 0 0 0.0001 0 0.0031 0 0 0
Punctate Nuclear 0.0017 0 0.0003 0 0.0013 0 0.0007 0 0.0011 0.0002 0 0 0.0001 0 0.0001 0 0.0004 0
Vacuole 0.2414 0.2418 0.2548 0.2108 0.2325 0.1995 0.2058 0.2155 0.2142 0.2195 0.171 0.1359 0.2118 0.2002 0.2045 0.2167 0.129 0.1365
Vacuole Periphery 0.7073 0.7371 0.7204 0.7492 0.7133 0.7808 0.7398 0.7665 0.7331 0.7379 0.8065 0.8491 0.766 0.7888 0.7637 0.7802 0.8412 0.8513

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 61.2205 42.7558 54.5698 65.4644 63.252 36.8076 44.8515 52.5196 51.4789 73.131
Translational Efficiency 0.9735 1.1953 0.9528 0.6802 0.9294 1.3085 0.9373 0.7669 0.8177 0.8457

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
240 1139 257 1454 1902 1460 507 1729 2142 2599 764 3183

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1181.78 1544.77 1653.12 1337.90 1446.18 1441.59 1486.10 1345.49 1416.56 1486.81 1542.28 1342.02
Standard Deviation 215.98 342.90 290.77 245.61 305.56 296.46 285.49 242.79 308.36 321.75 297.92 244.11
Intensity Change Log 2 — 0.386431 0.484230 0.179009 — -0.004586 0.039284 -0.104115 — 0.184445 0.256463 0.030113

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000398 0.000095 0.000149 0.000039 0.000008 0.000045 0.000093 0.000041 0.000052 0.000067 0.000112 0.000040
Bud Neck 0.002466 0.000348 0.003676 0.001574 0.000060 0.000752 0.002395 0.001892 0.000330 0.000575 0.002826 0.001747
Bud Site 0.007984 0.002570 0.001891 0.003430 0.000099 0.002432 0.002301 0.003017 0.000983 0.002493 0.002163 0.003206
Cell Periphery 0.000381 0.003592 0.000187 0.000696 0.001072 0.001096 0.000408 0.001187 0.000994 0.002190 0.000334 0.000962
Cytoplasm 0.002882 0.000427 0.000162 0.000757 0.000024 0.000211 0.000147 0.000348 0.000344 0.000305 0.000152 0.000535
Cytoplasmic Foci 0.014838 0.000297 0.001306 0.000551 0.000106 0.000493 0.002080 0.001415 0.001757 0.000407 0.001820 0.001020
Eisosomes 0.000021 0.000017 0.000045 0.000018 0.000008 0.000023 0.000050 0.000026 0.000010 0.000020 0.000048 0.000022
Endoplasmic Reticulum 0.002286 0.000268 0.000264 0.000471 0.000249 0.000464 0.000183 0.000805 0.000478 0.000378 0.000210 0.000652
Endosome 0.062220 0.000768 0.002074 0.004570 0.000315 0.001426 0.003575 0.007921 0.007252 0.001138 0.003070 0.006390
Golgi 0.025595 0.001042 0.001271 0.003523 0.000608 0.000795 0.004633 0.003798 0.003408 0.000903 0.003502 0.003672
Lipid Particles 0.000748 0.000458 0.000258 0.000502 0.000468 0.000706 0.000586 0.000833 0.000500 0.000597 0.000476 0.000682
Mitochondria 0.013773 0.005874 0.014611 0.006012 0.002772 0.006822 0.017771 0.008968 0.004004 0.006406 0.016708 0.007617
Mitotic Spindle 0.007484 0.000051 0.000166 0.001606 0.000010 0.000400 0.000246 0.000884 0.000848 0.000247 0.000219 0.001214
None 0.000134 0.000508 0.000430 0.000211 0.000151 0.000360 0.000487 0.000219 0.000149 0.000425 0.000468 0.000215
Nuclear Periphery 0.006007 0.000760 0.012056 0.001728 0.000841 0.001335 0.000850 0.001430 0.001420 0.001083 0.004620 0.001566
Nuclear Periphery Foci 0.003854 0.000318 0.002891 0.001320 0.000206 0.000580 0.000608 0.001000 0.000615 0.000465 0.001376 0.001146
Nucleolus 0.001188 0.001093 0.021651 0.001967 0.000554 0.002439 0.009010 0.002830 0.000625 0.001849 0.013262 0.002436
Nucleus 0.001348 0.000476 0.005049 0.001944 0.000283 0.001321 0.002177 0.003219 0.000402 0.000951 0.003143 0.002637
Peroxisomes 0.000918 0.000066 0.000669 0.000091 0.000020 0.000129 0.000714 0.000201 0.000120 0.000101 0.000699 0.000151
Vacuole 0.463517 0.164818 0.289206 0.404537 0.155021 0.251700 0.376980 0.444520 0.189586 0.213624 0.347454 0.426256
Vacuole Periphery 0.381957 0.816154 0.641989 0.564455 0.837123 0.726471 0.574704 0.515446 0.786124 0.765774 0.597338 0.537834

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.77 1.39 2.15 1.44 2.41 -3.49 -3.97 -4.67 0.29 2.31 -0.59 -2.20 0.58 1.44 3.35
Bud Neck 3.45 -1.03 1.46 -5.23 2.40 -2.53 -3.95 -6.87 -3.06 0.76 -1.23 -5.08 -7.46 -5.12 2.07
Bud Site 2.62 2.98 2.11 -1.10 -1.79 -3.03 -3.08 -5.73 -0.67 -0.82 -2.35 -2.03 -5.07 -1.27 -1.76
Cell Periphery -2.69 2.07 -2.43 2.42 -4.86 -0.14 6.12 -0.56 -0.39 -4.21 -2.23 7.13 0.25 2.28 -5.58
Cytoplasm 3.81 4.33 2.17 -1.52 -2.12 -2.85 -3.68 -5.22 -1.62 -2.96 0.50 2.55 -1.61 -1.83 -2.59
Cytoplasmic Foci 4.98 4.58 4.88 -2.25 1.80 -1.93 -3.64 -3.09 -1.98 0.96 3.73 -0.15 1.74 -2.37 1.77
Eisosomes 0.76 -2.77 0.66 -0.23 3.58 -4.46 -7.52 -5.78 -0.91 4.16 -4.01 -8.39 -5.36 -0.82 5.74
Endoplasmic Reticulum 2.72 2.64 2.44 -3.84 -1.18 -3.52 1.76 -4.95 -2.77 -5.46 1.07 2.53 -1.64 -3.84 -5.28
Endosome 8.48 8.27 7.92 -6.20 -3.02 -4.01 -5.06 -9.70 -7.92 -4.34 6.56 4.04 0.77 -9.95 -4.86
Golgi 5.20 5.14 4.66 -5.38 -4.23 -0.45 -4.43 -4.86 -5.33 0.84 3.76 -0.14 -0.39 -7.46 -0.25
Lipid Particles 1.29 2.12 1.09 -0.78 -4.34 -3.43 -1.80 -1.92 -0.64 -1.24 -1.86 0.44 -1.64 -0.76 -1.81
Mitochondria 1.90 -0.18 1.88 -0.10 2.94 -3.13 -4.31 -4.28 -1.26 2.41 -2.28 -4.96 -3.28 -1.10 3.53
Mitotic Spindle 2.81 2.77 1.96 -2.31 -2.18 -1.50 -2.08 -2.26 -1.11 -1.62 1.84 2.05 -1.06 -2.52 -2.67
None -3.07 -5.55 -2.04 2.39 3.79 -5.01 -8.92 -2.95 3.52 7.53 -4.69 -10.03 -2.94 3.55 7.99
Nuclear Periphery 1.92 -1.02 1.57 -2.99 2.04 -1.10 -0.03 -1.21 -0.24 -1.68 0.74 -1.80 -0.26 -1.76 1.77
Nuclear Periphery Foci 2.61 0.61 1.83 -2.81 1.50 -2.12 -4.84 -3.32 -1.49 -1.66 0.77 -2.02 -2.05 -3.03 0.57
Nucleolus 0.20 -4.24 -1.35 -3.16 4.10 -4.24 -5.91 -6.74 -0.81 4.22 -4.32 -6.71 -7.67 -1.90 5.72
Nucleus 1.64 -1.51 -0.28 -2.54 1.42 -3.56 -4.69 -5.39 -3.30 -1.68 -2.33 -3.47 -5.25 -4.11 0.58
Peroxisomes 3.38 0.81 3.28 -1.22 3.62 -3.80 -4.20 -4.38 -1.47 3.01 0.56 -4.62 -0.84 -1.73 4.42
Vacuole 17.55 8.15 3.42 -28.68 -7.47 -13.34 -20.52 -41.45 -23.13 -5.84 -3.88 -16.67 -38.22 -35.37 -8.45
Vacuole Periphery -22.92 -10.61 -9.48 27.74 4.35 14.27 22.14 43.15 23.47 4.68 2.89 17.62 35.78 35.11 5.80
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Protein of unknown function; proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene
Localization
Cell Percentages vacuolar membrane (90%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Vba4

Vba4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vba4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available