Standard name
Human Ortholog
Description Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Cytoplasm 0.95 0.99 0.99 0.99 0.98 0.93 0.93 – 0.84 0.85 0.78 0.74 0.99 0.99 0.99 – 0.96 – 0.75 0.89 0.76 0.8 0.76 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.06 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0.08 – 0.25 0.23 0.29 0.36 0 0 0 – 0 – 0.12 0.05 0.13 0.09 0.09 0.08
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 – 0 – 3 2 4 1 4 5
Bud 0 0 0 1 1 0 2 – 6 6 6 8 0 0 1 – 0 – 1 0 0 1 6 7
Bud Neck 0 1 4 7 12 5 12 – 6 12 4 5 0 0 0 – 1 – 3 1 2 1 1 1
Bud Site 0 0 0 0 0 0 0 – 2 0 1 2 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 1 1 1 1 2 – 0 3 3 0 0 2 0 – 0 – 0 0 1 0 2 2
Cytoplasm 232 250 218 291 417 329 551 – 360 443 300 360 290 466 444 – 150 – 191 222 159 157 275 265
Endoplasmic Reticulum 1 2 0 0 1 1 0 – 0 0 0 0 1 0 1 – 10 – 5 0 1 0 5 2
Endosome 0 1 0 0 0 0 1 – 0 1 1 6 1 0 0 – 0 – 4 2 0 1 3 3
Golgi 3 0 2 0 0 0 1 – 0 0 0 0 0 0 0 – 1 – 2 0 0 1 1 0
Mitochondria 6 2 0 9 3 43 48 – 108 122 111 174 1 2 4 – 4 – 30 11 28 17 30 28
Nucleus 0 0 0 0 0 2 3 – 3 5 3 4 0 0 1 – 1 – 0 0 0 0 3 1
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 – 0 – 0 0 0 0 2 0
Nucleolus 0 0 0 0 0 0 1 – 0 1 0 2 1 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 1 0 1 0 0 – 0 1 0 1 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 3 1 5 2 20 – 9 13 4 11 0 0 0 – 0 – 2 1 2 0 9 14
Vac/Vac Membrane 4 0 0 0 1 0 4 – 2 6 2 6 0 2 1 – 3 – 6 3 0 5 5 6
Unique Cell Count 243 253 221 295 424 354 592 427 524 385 486 293 469 448 156 256 250 209 198 362 346
Labelled Cell Count 246 256 229 311 442 383 645 496 613 435 579 294 473 452 170 256 250 209 198 362 346


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.5 4.3 4.3 4.9 3.4 4.1 – 3.4 3.6 3.4 3.6 4.3 4.5 4.9 4.7 5.0 4.8 4.1 4.5 4.4
Std Deviation (1e-4) 1.0 0.6 1.0 1.6 1.5 1.2 1.6 – 1.3 1.1 1.3 1.1 1.1 1.6 2.0 1.1 1.3 1.2 2.1 2.9 2.0
Intensity Change (Log2) – – – -0.01 0.18 -0.35 -0.06 – -0.32 -0.25 -0.33 -0.24 0.01 0.07 0.18 0.14 0.22 0.15 -0.07 0.07 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.4684 -1.303 -1.1935 -1.6533 -1.1307 -1.4702 -0.7379 -0.5952 -0.3796 -0.9595 -0.4372 -0.514 1.1807 1.0745 1.3754 1.2041 1.6082 1.158
Actin 0.0328 0.0005 0.0222 0.0001 0.0064 0.0008 0.0306 0.0002 0.0174 0.0053 0.0246 0.0025 0.0194 0 0.003 0.0062 0.0115 0.0039
Bud 0.0008 0.0041 0.0005 0 0.0014 0 0.0007 0.0004 0.0006 0.0001 0.001 0.0002 0.0001 0 0 0.0023 0 0
Bud Neck 0.0013 0.0003 0.0002 0 0.0008 0.0005 0.0025 0.0003 0.0081 0.0003 0.0034 0.0014 0.0005 0.0002 0.0034 0.0001 0.0001 0.0003
Bud Periphery 0.0013 0.0022 0.0005 0 0.0015 0 0.0015 0.0002 0.0011 0.0002 0.0018 0.0004 0.0001 0 0.0001 0.0061 0.0001 0.0001
Bud Site 0.0081 0.0088 0.0019 0 0.0332 0.0001 0.0079 0.0022 0.0053 0.0002 0.0055 0.0002 0.0004 0.0004 0.0003 0.0007 0.0001 0
Cell Periphery 0.0003 0.0002 0.0001 0 0.0002 0 0.0005 0.0001 0.0002 0 0.0002 0 0.0001 0 0.0001 0 0 0
Cytoplasm 0.0303 0.0515 0.043 0.0362 0.0436 0.1052 0.1609 0.2059 0.1683 0.2334 0.2232 0.2884 0.0523 0.0851 0.0748 0.0905 0.0236 0.1606
Cytoplasmic Foci 0.0129 0.012 0.0105 0.0015 0.015 0.019 0.0255 0.0085 0.0177 0.0079 0.0218 0.011 0.0101 0.0027 0.0043 0.0067 0.0034 0.0033
Eisosomes 0.0007 0.0002 0.0004 0.0001 0.0001 0 0.0007 0.0001 0.0003 0.0001 0.0004 0.0001 0.0004 0.0001 0.0002 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0034 0.0009 0.0018 0.0004 0.0015 0.0007 0.0031 0.0018 0.0027 0.0013 0.0029 0.0019 0.001 0.0011 0.0009 0.0011 0.0002 0.0011
Endosome 0.0148 0.0008 0.0077 0.0003 0.0145 0.0021 0.012 0.002 0.0096 0.0018 0.0105 0.003 0.0021 0.0009 0.0011 0.0013 0.0012 0.0018
Golgi 0.005 0.0002 0.0045 0 0.0037 0.0011 0.0039 0.0001 0.0017 0.0005 0.0039 0.0006 0.0006 0 0.0002 0.0008 0.0019 0.0006
Lipid Particles 0.0038 0.0029 0.0055 0.0001 0.007 0.0023 0.0184 0.0005 0.0046 0.0023 0.0118 0.0011 0.0019 0.0001 0.0003 0.0006 0.0126 0.0006
Mitochondria 0.0037 0.0005 0.004 0.0001 0.0028 0.0004 0.0056 0.0003 0.0019 0.0004 0.0024 0.0009 0.0003 0.0001 0.0002 0.0047 0.0005 0.0011
None 0.8677 0.9031 0.8764 0.9364 0.8172 0.8177 0.6614 0.7369 0.6851 0.6675 0.6059 0.642 0.9037 0.898 0.8855 0.8585 0.9292 0.8173
Nuclear Periphery 0.0019 0.0007 0.005 0.0014 0.012 0.0007 0.0067 0.0018 0.0077 0.0027 0.0097 0.0029 0.0011 0.0014 0.0013 0.0011 0.0004 0.0009
Nucleolus 0.0009 0.0006 0.0002 0.0003 0.002 0.0004 0.0017 0.0015 0.0019 0.0006 0.0033 0.0004 0.0003 0.0003 0.0006 0.0001 0.0001 0.0001
Nucleus 0.0017 0.0044 0.0011 0.0198 0.0054 0.0341 0.0254 0.025 0.0266 0.0543 0.0176 0.0329 0.0013 0.0066 0.0052 0.0019 0.001 0.0063
Peroxisomes 0.0018 0.0007 0.0034 0 0.0038 0.0058 0.0099 0.0002 0.0094 0.0123 0.0157 0.0002 0.0011 0 0.0002 0.0155 0.0117 0.0006
Punctate Nuclear 0.0043 0.005 0.0098 0.0032 0.0256 0.0084 0.0149 0.0105 0.0238 0.0083 0.0319 0.0089 0.0028 0.0021 0.0181 0.0012 0.0023 0.0012
Vacuole 0.0019 0.0004 0.0006 0.0001 0.0013 0.0002 0.0048 0.0013 0.0039 0.0006 0.0019 0.0006 0.0003 0.0004 0.0002 0.0003 0.0001 0.0002
Vacuole Periphery 0.0006 0.0001 0.0006 0 0.0006 0 0.0011 0.0001 0.0022 0.0001 0.0007 0.0003 0.0001 0.0001 0 0.0001 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.6727 15.021 24.7693 21.5539 35.0996 22.3917 20.2502 22.2098 17.5386 22.3334
Translational Efficiency 2.2003 3.9456 2.2041 2.4988 1.5953 2.3136 2.5663 2.6029 2.8241 2.5254

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
363 683 1631 1283 1976 2137 1807 1323 2339 2820 3438 2606

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 599.22 694.19 843.28 800.45 629.04 726.26 696.55 846.33 624.41 718.49 766.16 823.74
Standard Deviation 81.26 82.91 91.58 102.66 67.40 88.97 106.84 109.58 70.56 88.61 123.88 108.67
Intensity Change Log 2 — 0.212245 0.492926 0.417726 — 0.207334 0.147075 0.428069 — 0.209732 0.326155 0.423032

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000154 0.000733 0.000181 0.000264 0.000095 0.000257 0.000141 0.001197 0.000104 0.000372 0.000160 0.000738
Bud Neck 0.037775 0.033703 0.003161 0.007875 0.024711 0.029748 0.001638 0.009697 0.026739 0.030706 0.002361 0.008800
Bud Site 0.004382 0.013336 0.005591 0.014670 0.003326 0.013137 0.001498 0.037898 0.003490 0.013185 0.003440 0.026462
Cell Periphery 0.000655 0.000206 0.000078 0.000178 0.000138 0.000154 0.000119 0.000254 0.000218 0.000166 0.000100 0.000216
Cytoplasm 0.297069* 0.322007* 0.196679* 0.291752* 0.423283* 0.449765* 0.382157* 0.337999* 0.403695* 0.418822* 0.294166* 0.315230*
Cytoplasmic Foci 0.316668* 0.297308* 0.003230 0.007368 0.250458* 0.169626 0.006138 0.015159 0.260733* 0.200550* 0.004759 0.011323
Eisosomes 0.000553 0.000185 0.000022 0.000038 0.000173 0.000095 0.000023 0.000046 0.000232 0.000117 0.000022 0.000042
Endoplasmic Reticulum 0.001454 0.001739 0.003134 0.003627 0.000604 0.000506 0.001728 0.002846 0.000736 0.000805 0.002395 0.003230
Endosome 0.011711 0.022927 0.001165 0.002312 0.007198 0.009674 0.000812 0.006683 0.007899 0.012884 0.000979 0.004531
Golgi 0.007657 0.006887 0.000049 0.001627 0.002720 0.003535 0.000049 0.004730 0.003486 0.004347 0.000049 0.003202
Lipid Particles 0.040860 0.012405 0.000783 0.001225 0.011569 0.005150 0.000718 0.001357 0.016115 0.006907 0.000749 0.001292
Mitochondria 0.070653 0.019879 0.000865 0.001513 0.008542 0.014288 0.000475 0.003818 0.018182 0.015643 0.000660 0.002683
Mitotic Spindle 0.000207 0.002950 0.003582 0.015889 0.001270 0.011808 0.003792 0.032216 0.001105 0.009663 0.003692 0.024178
None 0.007302 0.003719 0.003990 0.009287 0.003250 0.005867 0.013888 0.012174 0.003879 0.005347 0.009192 0.010753
Nuclear Periphery 0.000571 0.000701 0.002366 0.002634 0.000484 0.000705 0.001148 0.002542 0.000497 0.000704 0.001726 0.002587
Nuclear Periphery Foci 0.000474 0.000631 0.000481 0.000928 0.000179 0.000316 0.000533 0.001185 0.000224 0.000392 0.000508 0.001059
Nucleolus 0.002772 0.002951 0.000573 0.000654 0.001324 0.001910 0.000713 0.000699 0.001549 0.002162 0.000646 0.000677
Nucleus 0.150660* 0.167177* 0.753664* 0.579420* 0.228082* 0.236141* 0.568092* 0.453901* 0.216067* 0.219438* 0.656128* 0.515697*
Peroxisomes 0.025446 0.036584 0.000131 0.000216 0.008151 0.009631 0.000119 0.002365 0.010835 0.016159 0.000125 0.001307
Vacuole 0.019174 0.050961 0.020095 0.051764 0.023569 0.035652 0.016130 0.070417 0.022887 0.039359 0.018011 0.061233
Vacuole Periphery 0.003802 0.003010 0.000180 0.006762 0.000876 0.002035 0.000090 0.002817 0.001330 0.002271 0.000133 0.004759

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.38 -3.06 -5.25 3.25 -3.41 -2.67 -8.59 -4.39 -2.76 -3.38 -5.10 -10.24 -6.39 -1.71 -4.16
Bud Neck 0.62 6.17 5.55 7.26 -3.89 -2.14 13.13 8.67 11.29 -8.50 -1.78 14.09 10.77 13.82 -8.73
Bud Site -5.54 -0.05 -2.63 1.98 -2.45 -7.85 3.22 -5.48 -1.14 -6.10 -9.50 0.68 -6.35 0.30 -6.35
Cell Periphery 4.52 5.84 5.17 3.60 -6.33 -1.40 2.63 -0.99 0.12 -2.46 2.85 7.09 3.86 1.58 -5.54
Cytoplasm -1.44 9.75 5.10 7.70 -7.20 -3.14 10.40 19.35 21.87 7.97 -1.90 21.55 23.39 25.39 1.94
Cytoplasmic Foci 1.46 28.56 28.31 33.08 -4.56 12.00 47.84 46.61 36.62 -3.25 9.72 54.95 54.07 47.67 -4.95
Eisosomes 3.83 5.48 5.20 11.93 -9.55 10.42 22.18 17.27 8.27 -12.31 6.98 12.62 10.86 13.79 -15.36
Endoplasmic Reticulum -0.78 -9.10 -11.24 -11.12 -3.10 0.90 -15.35 -20.36 -19.46 -7.67 -0.67 -21.78 -26.85 -24.25 -7.37
Endosome -4.26 6.96 6.58 9.59 -1.97 -2.97 13.07 5.95 7.78 -4.77 -5.66 14.43 10.02 13.02 -5.19
Golgi 0.51 5.43 4.82 5.30 -1.36 -1.34 8.44 1.93 2.69 -2.41 -1.41 9.92 4.92 5.55 -2.76
Lipid Particles 6.05 8.97 8.86 7.74 -5.07 8.35 16.38 15.29 8.98 -7.41 8.94 16.69 16.02 11.69 -8.84
Mitochondria 5.66 8.02 7.98 7.44 -1.46 -3.61 8.77 6.77 9.15 -4.42 1.30 10.76 10.13 11.89 -4.51
Mitotic Spindle -4.21 -4.28 -5.47 -3.95 -3.40 -6.07 -2.81 -7.91 -3.80 -6.40 -6.38 -3.83 -9.19 -3.91 -7.14
None 1.32 1.37 0.28 -2.23 -2.53 -3.17 -8.32 -5.28 -2.08 2.40 -1.87 -5.34 -4.04 -2.26 0.30
Nuclear Periphery -1.49 -23.74 -19.69 -18.11 -5.01 -1.55 -15.75 -21.33 -16.84 -15.26 -1.90 -27.66 -29.09 -22.28 -14.63
Nuclear Periphery Foci -0.66 -0.71 -2.32 -3.94 -4.19 -0.98 -8.14 -8.41 -5.22 -4.09 -1.55 -7.79 -10.86 -6.29 -5.77
Nucleolus -0.77 3.91 3.85 4.73 -0.30 -2.71 4.21 3.44 6.63 -1.35 -3.02 5.87 5.35 8.20 -1.12
Nucleus -1.44 -54.19 -36.71 -38.77 15.92 -1.14 -44.51 -31.49 -29.99 7.17 -0.56 -71.63 -48.78 -47.82 15.21
Peroxisomes -2.41 9.36 9.35 10.03 -1.30 -1.70 13.04 10.61 11.13 -2.35 -4.27 15.74 14.78 14.66 -2.47
Vacuole -8.03 -6.20 -18.00 -9.66 -14.00 -6.71 -3.08 -19.23 -16.52 -17.56 -9.89 -6.40 -26.28 -20.49 -22.46
Vacuole Periphery 1.17 6.48 1.15 0.47 -2.13 -4.25 9.20 -0.13 2.58 -2.42 -3.71 10.43 -0.59 1.00 -2.79
Standard name
Human Ortholog
Description Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dpb4

Dpb4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dpb4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available