Standard name
Human Ortholog
Description S/T protein kinase; regulates polarized exocytosis and the Ire1p-mediated unfolded protein response; regulates HAC1 mRNA translocation, splicing and translation with KIN2 during ER stress; direct phosphorylation of the substrate Sec9p (S190) is enhanced by prior substrate priming (S192); localizes to the cytoplasmic face of the plasma membrane; activation loop phosphorylation (T302) required for full kinase activity; orthologous to MARK/PAR-1, AMP-activated protein kinase (AMPK) family members
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0.11 0.08 0.06 0
Bud 0 0 0 0 0 0 0 0 – 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0.08 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0.1 0.07 0 0.06 0.05 – 0.05 0.05 0.11 0.13 0.13 0.11 0.1 0 0 0 0 0.05 0 0 0
Cytoplasm 0.94 0.93 0.92 0.92 0.88 0.83 0.73 0.72 – 0.65 0.62 0.58 0.96 0.96 0.93 0.86 0.91 0.88 0.78 0.84 0.68 0.68 0.76 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0.06 0 0.05 0 0 0 0.05 0.09 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0.08 0.2 0.19 0.2 – 0.31 0.34 0.3 0 0 0 0.07 0 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.12 0.11 – 0.08 0.09 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 11 2 18 7 7 2 9 6 – 9 4 18 8 8 4 1 1 0 26 6 65 27 28 21
Bud 9 7 21 8 10 15 25 20 – 23 22 32 8 8 7 1 0 0 4 4 9 11 16 13
Bud Neck 2 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 31 8 24 27 18 10
Bud Site 0 1 1 0 1 3 1 1 – 1 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 10 1 30 33 35 22 48 42 – 34 22 61 45 45 30 4 0 0 7 1 30 11 9 9
Cytoplasm 367 255 544 308 461 515 581 571 – 430 296 334 340 340 259 36 42 15 333 248 408 232 389 418
Endoplasmic Reticulum 13 9 8 9 10 22 15 17 – 39 16 30 4 6 10 2 4 1 4 1 20 6 9 9
Endosome 0 0 6 2 13 5 12 6 – 0 7 9 0 0 0 0 0 0 5 2 11 2 4 1
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 1 2 1 1 2
Mitochondria 0 13 10 15 40 124 153 160 – 203 163 174 0 0 0 3 2 1 3 6 14 12 11 10
Nucleus 0 1 1 2 2 3 3 6 – 4 0 1 0 0 3 1 2 0 0 3 0 0 0 0
Nuclear Periphery 0 1 1 0 1 1 1 0 – 5 0 4 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 3 0 0 0 0 6 – 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 2 1 0 2 4 1 – 1 0 3 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 7 0 3 0 1 1 – 1 0 4 1 1 0 3 0 2 0 1 4 0 2 3
Vac/Vac Membrane 0 1 6 3 17 10 96 86 – 54 45 66 0 0 0 0 0 0 8 5 2 5 7 3
Unique Cell Count 391 275 594 335 524 619 800 789 657 474 575 355 354 278 42 46 17 428 294 602 342 509 518
Labelled Cell Count 412 292 658 388 600 724 949 923 805 577 739 407 408 313 51 51 19 428 294 602 342 509 518


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 5.5 5.5 5.8 6.2 5.9 6.2 6.4 – 5.7 5.8 6.2 7.5 7.5 7.7 5.9 5.6 7.6 7.0 7.2 7.8
Std Deviation (1e-4) 0.7 1.1 1.3 1.4 1.7 1.2 1.5 1.5 – 1.5 1.5 1.7 0.8 0.8 0.9 0.9 1.0 1.4 1.3 1.4 1.7
Intensity Change (Log2) – – – 0.06 0.17 0.1 0.15 0.22 – 0.04 0.08 0.17 0.44 0.44 0.48 0.09 0.02 0.46 0.34 0.38 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.9 -1.9 -3.7 -2.6 -3.2 – -2.0 -2.5 0.1 -0.7 -0.7 -1.4 0 0 0
Bud -1.0 -1.7 -1.1 -0.4 -1.1 – 0 0.9 1.7 -1.1 -1.1 -0.8 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 2.8 1.2 -1.3 0.8 0.2 – 0.1 -0.3 3.5 4.2 4.2 3.1 0 0 0
Cytoplasm 0.2 -2.0 -4.4 -8.9 -8.9 – -11.1 -11.5 -13.2 2.5 2.6 0.8 -1.3 -0.1 -0.5
Endoplasmic Reticulum 1.5 0.7 2.5 0.8 1.1 – 4.3 2.2 3.7 0 0.4 2.2 0 0 0
Endosome 0 1.9 -0.4 0.8 -0.5 – 0 0.7 0.8 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 2.5 4.8 10.2 10.0 10.4 – 13.7 14.4 13.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 -0.9 0 0 0 0 0 0
Vacuole 0 2.6 0.9 7.8 7.3 – 6.0 6.5 7.4 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6763 3.6131 3.2294 2.8124 3.2517 3.3916 3.5901 4.5922 4.1554 3.7797 3.5354 4.3858 2.1365 3.5758 3.0248 3.0318 2.9109 3.1307
Actin 0.0548 0.011 0.039 0.0134 0.1438 0.0255 0.0145 0.0126 0.0252 0.0473 0.0225 0.0142 0.1293 0.0471 0.0236 0.023 0.1154 0.0066
Bud 0.0061 0.0051 0.0423 0.0053 0.0044 0.0015 0.0099 0.0044 0.0409 0.0319 0.0023 0.0014 0.0209 0.0009 0.0311 0.0306 0.0171 0.0011
Bud Neck 0.0146 0.0011 0.0119 0.001 0.0052 0.2063 0.0099 0.0024 0.0015 0.0014 0.0217 0.2184 0.0027 0.0039 0.0008 0.0003 0.0024 0.2389
Bud Periphery 0.0191 0.0068 0.1027 0.0118 0.009 0.0029 0.0133 0.0056 0.075 0.0921 0.0038 0.0012 0.0231 0.0005 0.0718 0.2932 0.0309 0.0016
Bud Site 0.0341 0.039 0.0287 0.0012 0.0045 0.0018 0.0376 0.0941 0.0193 0.0143 0.0046 0.0018 0.0419 0.1215 0.0033 0.0012 0.003 0.0004
Cell Periphery 0.0085 0.0023 0.0039 0.0025 0.0033 0.0018 0.0079 0.0062 0.0031 0.0044 0.0027 0.0016 0.0048 0.0028 0.002 0.0102 0.0045 0.0028
Cytoplasm 0.6293 0.8329 0.6178 0.8494 0.6165 0.6705 0.7389 0.7844 0.6881 0.6591 0.829 0.6379 0.6572 0.7483 0.6438 0.5889 0.778 0.6804
Cytoplasmic Foci 0.0221 0.0053 0.0226 0.0127 0.0276 0.0075 0.013 0.008 0.0089 0.0064 0.005 0.0132 0.0215 0.0091 0.0223 0.0038 0.0086 0.0021
Eisosomes 0.0004 0.0001 0.0003 0.0002 0.0004 0.0001 0.0005 0.0001 0.0021 0.0006 0.0001 0.0001 0.0006 0.0005 0.0002 0.0001 0.0002 0
Endoplasmic Reticulum 0.0082 0.0029 0.0039 0.0072 0.007 0.0019 0.0051 0.0025 0.003 0.0082 0.0037 0.002 0.0046 0.0028 0.0036 0.006 0.0064 0.0022
Endosome 0.0175 0.0014 0.0107 0.0113 0.0368 0.0033 0.0009 0.001 0.0024 0.0028 0.0012 0.0018 0.029 0.0006 0.0134 0.0013 0.0017 0.0009
Golgi 0.0077 0.0007 0.0075 0.0032 0.0189 0.0021 0.0007 0.0008 0.0014 0.0022 0.0009 0.0006 0.006 0.001 0.0071 0.0007 0.0019 0.0002
Lipid Particles 0.0181 0.0002 0.0073 0.0021 0.0141 0.0014 0.0006 0.0002 0.0005 0.0007 0.0002 0.0003 0.0147 0.0007 0.0159 0.0005 0.0006 0.0001
Mitochondria 0.0131 0.0003 0.0126 0.0124 0.0096 0.0007 0.0002 0.0002 0.0007 0.0009 0.0002 0.0001 0.0007 0.0001 0.0031 0.0001 0.0003 0.0003
None 0.0789 0.083 0.0706 0.0517 0.0589 0.0576 0.1425 0.0741 0.1224 0.12 0.0981 0.1021 0.009 0.0555 0.1308 0.0375 0.0266 0.0594
Nuclear Periphery 0.0115 0.0013 0.0012 0.0026 0.0139 0.004 0.0005 0.0003 0.0006 0.0012 0.0006 0.0004 0.0096 0.0007 0.0005 0.0006 0.0006 0.0011
Nucleolus 0.0013 0.0003 0.0004 0.0003 0.0004 0.0007 0.0002 0.0001 0.0002 0.0003 0.0001 0.0001 0.0003 0.0001 0.0002 0 0 0
Nucleus 0.012 0.0026 0.0016 0.0022 0.0034 0.0026 0.0011 0.0011 0.0015 0.0023 0.0014 0.0008 0.0011 0.0008 0.0006 0.0005 0.0004 0.0011
Peroxisomes 0.0161 0.0011 0.0089 0.0036 0.0136 0.0021 0.0004 0.0007 0.0008 0.0006 0.0002 0.0004 0.0036 0.0009 0.0231 0 0.0005 0.0001
Punctate Nuclear 0.0217 0.0013 0.0031 0.0022 0.0055 0.0048 0.0009 0.0004 0.0012 0.0007 0.0007 0.0011 0.018 0.0021 0.0009 0.0002 0.0004 0.0001
Vacuole 0.0037 0.0012 0.0025 0.0031 0.0018 0.0007 0.0011 0.0008 0.001 0.0023 0.001 0.0004 0.0009 0.0002 0.0015 0.0012 0.0005 0.0004
Vacuole Periphery 0.0011 0.0001 0.0006 0.0007 0.0014 0.0004 0.0001 0.0001 0.0001 0.0002 0.0001 0 0.0005 0 0.0004 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.2108 13.2752 21.076 35.4018 22.1644 35.2066 26.3768 35.3944 37.886 34.4998
Translational Efficiency 0.7082 0.8959 0.6985 0.5924 0.6913 0.6432 0.5328 0.4343 0.5456 0.5353

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description S/T protein kinase; regulates polarized exocytosis and the Ire1p-mediated unfolded protein response; regulates HAC1 mRNA translocation, splicing and translation with KIN2 during ER stress; direct phosphorylation of the substrate Sec9p (S190) is enhanced by prior substrate priming (S192); localizes to the cytoplasmic face of the plasma membrane; activation loop phosphorylation (T302) required for full kinase activity; orthologous to MARK/PAR-1, AMP-activated protein kinase (AMPK) family members
Localization
Cell Percentages cytoplasm (40%), ER (2%), mixed (45%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Kin1

Kin1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kin1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available