Standard name
Human Ortholog
Description Transcription factor; component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion; involved in diauxic shift
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.24 0.19 0.2 0.19 0.15 0.25 0.21 0.22 0.26 0.16 0.15 0.11 0.37 0.45 0.4 0.15 0.11 0.14 0.15 0.13 0.08 0.1 0.16 0.19
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.53 0 0.1 0.25 0.28 0.28 0.24 0.66 0.55 0.71 0.67 0 0 0.06 0 0 0 0 0.05 0.05 0 0 0.06
Nucleus 0.69 0.53 0.74 0.72 0.74 0.54 0.59 0.59 0.33 0.58 0.57 0.57 0.6 0.54 0.5 0.76 0.8 0.75 0.64 0.56 0.68 0.58 0.57 0.41
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0 0 0 0.05 0 0.05 0 0 0 0 0.05 0 0.06 0.09 0.09 0.09 0.12 0.15 0.08 0.14 0.13 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 3 0 1 2 1 1 1 0 3 3 1 0 0 0 0 0 1 1 2 4 0 1 1
Bud Neck 0 0 4 2 1 4 6 5 0 4 0 3 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 1 0 0 1 1 4 1 3 1 6 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 10 43 38 27 21 47 48 58 46 30 31 29 16 45 44 29 24 28 10 30 15 4 17 21
Endoplasmic Reticulum 0 2 0 0 1 0 0 0 0 0 0 1 0 0 0 11 8 8 0 0 0 0 0 1
Endosome 0 0 2 0 0 3 4 3 0 1 0 0 0 0 0 1 2 2 0 4 3 0 1 4
Golgi 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 1 1 0 2 0
Mitochondria 8 119 8 14 34 52 64 63 119 106 150 171 1 0 6 8 4 6 2 13 10 1 2 6
Nucleus 29 120 144 101 101 101 138 158 59 111 120 145 26 55 55 149 172 155 47 135 138 23 62 46
Nuclear Periphery 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 1 0 0 0 1 1 1 0 1
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 4 2 2 1 1 9 8 1 0 3 6 0 1 0 1 1 2 1 10 6 0 2 1
Vac/Vac Membrane 3 8 4 2 0 10 10 13 6 5 5 3 2 3 7 17 20 18 8 35 16 5 14 22
Unique Cell Count 42 225 194 140 136 188 232 268 179 192 210 254 43 101 109 195 214 206 74 244 204 40 110 114
Labelled Cell Count 50 300 205 149 162 221 284 311 234 263 318 366 45 104 112 217 234 222 74 244 204 40 110 114


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 3.5 5.3 4.8 4.1 4.1 4.3 4.5 3.3 3.8 3.6 3.6 5.7 5.3 4.5 5.3 6.6 6.3 – 6.9 6.4
Std Deviation (1e-4) 1.2 0.8 1.1 1.0 1.1 0.9 1.2 1.5 0.8 1.0 1.0 1.0 3.0 1.7 1.5 1.2 1.6 1.3 – 2.8 2.4
Intensity Change (Log2) – – – -0.14 -0.38 -0.36 -0.3 -0.23 -0.67 -0.47 -0.55 -0.56 0.09 -0.01 -0.23 -0.01 0.31 0.23 – 0.37 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.9 3.1 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0092 0.0005 0.0408 0.0003 0.0001 0.0027 0.0429 0.0343 0.0002 0 0.0067 0.0003 0.0015 0.0001 0 0 0 0.0035
Bud 0.0004 0.0002 0.0045 0 0.0001 0.0001 0.0018 0.0002 0 0 0.0001 0.0002 0.002 0.0001 0 0 0 0.0003
Bud Neck 0.0017 0.0006 0.001 0.0003 0.0005 0.0027 0.0123 0.0002 0.0001 0 0.0002 0.0008 0.0064 0.0001 0 0 0 0.0005
Bud Periphery 0.0012 0.0003 0.0067 0 0 0.0002 0.0042 0.0005 0 0 0.0004 0.0002 0.0011 0.0001 0 0 0 0.0009
Bud Site 0.0018 0.0115 0.0307 0.0001 0.0001 0.001 0.0069 0.0004 0.0001 0 0.0001 0.0001 0.1233 0.0006 0 0 0 0.0012
Cell Periphery 0.001 0.0002 0.0006 0 0 0.0001 0.0024 0.0002 0 0 0.0001 0 0.0011 0.0001 0 0 0 0.0003
Cytoplasm 0.0085 0.0308 0.0203 0.0048 0.1189 0.0199 0.0112 0.0009 0.0085 0.0007 0.0231 0.1165 0.0488 0.003 0 0.001 0.0016 0.0004
Cytoplasmic Foci 0.0145 0.0161 0.0163 0.0003 0.0054 0.0557 0.0191 0.012 0.0009 0 0.0004 0.0137 0.2562 0 0 0.0555 0.0003 0.0024
Eisosomes 0.0018 0.0002 0.0004 0.0008 0.0003 0.0001 0.0011 0.0012 0.0003 0 0.0004 0 0.0001 0 0 0 0 0.0002
Endoplasmic Reticulum 0.0047 0.0073 0.0089 0.0001 0.0076 0.001 0.0155 0.0073 0.0001 0 0.0017 0.0033 0.0067 0.0001 0 0 0 0.0016
Endosome 0.0187 0.0038 0.0868 0.0001 0.0064 0.0051 0.1144 0.0338 0.0002 0 0.0045 0.0087 0.3004 0.0001 0 0.0031 0 0.0288
Golgi 0.0146 0.0013 0.0225 0 0.0001 0.0017 0.0293 0.0562 0 0 0.0061 0.0004 0.0291 0 0 0.0036 0 0.0108
Lipid Particles 0.0398 0.0044 0.0564 0.0001 0.0005 0.0023 0.1354 0.0805 0.0001 0 0.0087 0.0016 0.0702 0 0 0.4211 0.0001 0.0294
Mitochondria 0.018 0.0167 0.0071 0.0003 0.0003 0.0009 0.0134 0.2463 0.0002 0.0001 0.0801 0.0004 0.0046 0.0006 0.0001 0.0018 0.0001 0.0068
None 0.2078 0.2792 0.1508 0.3664 0.2372 0.1156 0.2085 0.0024 0.2639 0.0001 0.0198 0.2421 0.0103 0.0003 0 0.4989 0.3314 0.0008
Nuclear Periphery 0.0062 0.0304 0.0161 0.0047 0.0302 0.0063 0.0722 0.0034 0.0024 0.0017 0.0157 0.011 0.0087 0.0055 0.0005 0 0.0003 0.0272
Nucleolus 0.0039 0.0037 0.0072 0.0212 0.0032 0.016 0.0128 0.0004 0.0025 0.0018 0.0024 0.0034 0.0145 0.0049 0.0008 0.003 0.0019 0.01
Nucleus 0.6236 0.5773 0.4758 0.5563 0.5271 0.7373 0.2104 0.4998 0.7044 0.995 0.8198 0.575 0.0172 0.9811 0.9986 0.0001 0.664 0.8595
Peroxisomes 0.0121 0.01 0.0301 0 0 0.0134 0.0116 0.0149 0.0001 0 0.0004 0.0004 0.0197 0 0 0.0101 0 0.0012
Punctate Nuclear 0.003 0.003 0.0059 0.044 0.0495 0.0158 0.0422 0.0007 0.0159 0.0006 0.0041 0.0197 0.0322 0.0027 0 0.0003 0.0002 0.0062
Vacuole 0.0047 0.0021 0.0074 0.0001 0.0104 0.0019 0.0255 0.001 0.0001 0 0.0008 0.0016 0.0421 0.0004 0 0.0006 0 0.0037
Vacuole Periphery 0.0029 0.0005 0.0037 0 0.0019 0.0004 0.0068 0.0034 0 0 0.0044 0.0005 0.0039 0.0002 0 0.0006 0 0.0044

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.4709 11.8581 7.1993 9.8589 8.5993 7.2314 10.0822 9.1957 10.9442 12.9506
Translational Efficiency 0.5176 0.5171 0.8616 0.526 0.7805 0.9259 0.8253 0.6533 0.4866 0.7529

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1809 326 1993 1779 1223 453 240 1412 3032 779 2233 3191

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 714.71 673.23 805.84 839.31 679.89 857.01 882.30 884.61 700.66 780.10 814.06 859.36
Standard Deviation 107.32 108.32 108.75 115.98 84.40 101.20 101.96 121.89 100.18 138.15 110.61 120.75
Intensity Change Log 2 — -0.086259 0.173135 0.231846 — 0.334011 0.375968 0.379740 — 0.133907 0.275583 0.305841

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000258 0.001254 0.000350 0.000709 0.000104 0.000363 0.000603 0.000688 0.000196 0.000736 0.000377 0.000700
Bud Neck 0.009501 0.022984 0.015171 0.021353 0.005273 0.012935 0.004927 0.021987 0.007795 0.017140 0.014070 0.021634
Bud Site 0.003822 0.017886 0.004334 0.012332 0.001354 0.003745 0.001059 0.017281 0.002826 0.009663 0.003982 0.014522
Cell Periphery 0.000201 0.000317 0.000111 0.000227 0.000244 0.000275 0.000095 0.000227 0.000218 0.000293 0.000110 0.000227
Cytoplasm 0.102317* 0.120430* 0.022631 0.091962 0.083055 0.085990 0.019997 0.070862 0.094547 0.100402* 0.022348 0.082625
Cytoplasmic Foci 0.029487 0.059026 0.000341 0.003351 0.028013 0.050739 0.000565 0.003627 0.028892 0.054207 0.000365 0.003473
Eisosomes 0.000048 0.000094 0.000016 0.000030 0.000048 0.000079 0.000037 0.000030 0.000048 0.000085 0.000019 0.000030
Endoplasmic Reticulum 0.001648 0.005540 0.004094 0.005688 0.001832 0.002321 0.006851 0.008512 0.001723 0.003668 0.004391 0.006938
Endosome 0.003649 0.018030 0.000482 0.003464 0.002777 0.007315 0.000617 0.003185 0.003297 0.011799 0.000497 0.003341
Golgi 0.000806 0.004932 0.000054 0.001239 0.000555 0.005240 0.000057 0.001175 0.000705 0.005112 0.000054 0.001211
Lipid Particles 0.004607 0.005457 0.000229 0.000722 0.004065 0.005958 0.000665 0.000671 0.004388 0.005748 0.000276 0.000699
Mitochondria 0.005156 0.017765 0.001154 0.004212 0.003695 0.015870 0.000676 0.005603 0.004566 0.016663 0.001102 0.004827
Mitotic Spindle 0.003292 0.007039 0.003245 0.021947 0.001207 0.008797 0.003545 0.012861 0.002451 0.008061 0.003277 0.017926
None 0.015738 0.032423 0.003432 0.008166 0.012626 0.011736 0.012227 0.006117 0.014483 0.020393 0.004377 0.007259
Nuclear Periphery 0.001281 0.000599 0.001207 0.002818 0.001241 0.000845 0.001115 0.002292 0.001265 0.000742 0.001197 0.002585
Nuclear Periphery Foci 0.000507 0.002066 0.000229 0.000777 0.000302 0.001253 0.000570 0.000699 0.000424 0.001593 0.000265 0.000743
Nucleolus 0.006856 0.020384 0.002059 0.003014 0.011476 0.011485 0.003504 0.002487 0.008719 0.015209 0.002215 0.002781
Nucleus 0.800424* 0.620103* 0.936628* 0.784431* 0.833555* 0.753574* 0.935250* 0.814142* 0.813788* 0.697718* 0.936480* 0.797578*
Peroxisomes 0.002988 0.016739 0.000148 0.000979 0.003699 0.013673 0.000185 0.000699 0.003274 0.014956 0.000152 0.000855
Vacuole 0.006961 0.024460 0.003838 0.027469 0.004359 0.006823 0.007323 0.024814 0.005911 0.014204 0.004213 0.026294
Vacuole Periphery 0.000457 0.002471 0.000247 0.005113 0.000521 0.000985 0.000131 0.002041 0.000483 0.001607 0.000234 0.003753

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.22 -1.04 -2.74 1.83 -3.12 -2.95 -6.35 -12.36 -4.35 0.93 -3.56 -3.02 -5.55 -0.14 -4.54
Bud Neck -3.56 -4.34 -6.88 0.38 -2.70 -3.14 -2.32 -10.31 -3.70 -7.30 -4.32 -6.11 -11.47 -2.26 -4.29
Bud Site -4.20 -0.67 -4.10 2.49 -3.73 -3.14 -1.84 -6.72 -5.16 -6.27 -4.48 -1.74 -7.58 -1.14 -5.94
Cell Periphery -2.81 4.34 1.53 3.74 -2.66 -0.63 3.67 1.40 1.81 -3.04 -2.25 5.60 2.02 3.69 -4.07
Cytoplasm -1.35 16.49 4.53 3.51 -12.96 -0.24 9.10 2.90 2.29 -7.34 -0.65 18.99 5.48 3.85 -15.23
Cytoplasmic Foci -4.00 13.56 12.54 8.39 -4.88 -3.32 10.07 9.13 7.62 -3.83 -5.04 16.86 15.44 11.14 -6.08
Eisosomes -5.72 8.15 3.03 7.69 -11.31 -3.14 -0.61 2.79 4.45 2.39 -5.69 9.57 4.05 7.82 -10.33
Endoplasmic Reticulum -5.14 -12.40 -16.22 -0.88 -6.59 -1.51 -8.28 -16.71 -14.72 -0.95 -5.34 -14.43 -23.20 -9.27 -9.90
Endosome -3.51 4.99 2.23 3.95 -5.26 -1.90 3.10 0.52 2.08 -3.45 -3.82 6.21 2.12 4.38 -6.88
Golgi -2.95 3.98 0.77 3.11 -2.73 -3.15 3.10 -0.43 3.00 -2.00 -4.19 5.03 0.28 4.22 -3.31
Lipid Particles -0.81 8.37 7.43 5.68 -6.18 -1.95 6.07 6.55 5.62 0.66 -1.91 11.04 9.76 7.83 -6.18
Mitochondria -3.11 5.13 2.03 3.61 -6.13 -3.82 2.94 -0.55 3.61 -3.89 -4.83 5.62 0.94 5.19 -5.93
Mitotic Spindle -1.42 -0.51 -5.93 -2.72 -5.71 -2.65 -1.07 -5.85 -1.63 -3.85 -2.78 -1.44 -8.21 -3.01 -6.99
None -2.45 7.63 5.62 4.15 -3.77 0.53 0.88 5.08 3.45 1.16 -1.74 8.78 7.47 5.10 -2.56
Nuclear Periphery 4.54 -1.84 -9.61 -13.02 -8.89 1.11 -0.92 -5.48 -10.42 -6.61 3.06 -1.69 -10.35 -16.41 -11.23
Nuclear Periphery Foci -1.88 2.16 -0.72 1.75 -3.15 -1.39 -2.04 -3.44 0.60 -0.43 -2.20 1.46 -2.56 1.64 -4.14
Nucleolus -3.44 8.20 6.64 4.57 -1.21 0.00 6.09 7.63 5.62 2.07 -3.27 10.56 10.18 6.70 -0.53
Nucleus 8.13 -17.04 -0.81 -8.58 17.92 4.54 -7.12 2.19 -3.31 9.50 8.30 -19.19 0.69 -8.09 22.04
Peroxisomes -4.03 7.23 5.74 4.81 -1.86 -3.84 4.60 4.15 5.17 -1.52 -5.64 8.08 6.88 7.06 -2.49
Vacuole -4.66 2.54 -11.21 -1.55 -12.66 -2.09 -3.41 -12.08 -10.82 -6.06 -4.70 0.44 -16.26 -7.92 -16.49
Vacuole Periphery -4.83 3.00 -3.38 -0.05 -3.74 -2.33 3.12 -2.20 -0.83 -3.35 -5.43 3.47 -4.01 -0.87 -4.69
Standard name
Human Ortholog
Description Transcription factor; component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion; involved in diauxic shift
Localization
Cell Percentages nucleus (88%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Ino2

Ino2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ino2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available