Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | – | – | 3 | 8 | 14 | 11 | 13 | 23 |
Bud | 4 | 5 | 7 | 7 | 9 | 27 | 29 | 23 | 31 | 20 | 18 | 11 | 2 | 0 | 0 | 0 | – | – | 1 | 1 | 2 | 7 | 7 | 16 |
Bud Neck | 1 | 1 | 5 | 1 | 4 | 4 | 2 | 3 | 3 | 1 | 0 | 3 | 2 | 13 | 2 | 0 | – | – | 2 | 2 | 3 | 4 | 11 | 12 |
Bud Site | 0 | 0 | 1 | 1 | 0 | 2 | 8 | 7 | 6 | 9 | 9 | 9 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 2 | 3 | 1 | 0 | 4 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | – | – | 0 | 1 | 2 | 2 | 6 | 8 |
Cytoplasm | 106 | 144 | 187 | 97 | 110 | 140 | 143 | 131 | 90 | 67 | 92 | 72 | 482 | 351 | 480 | 30 | – | – | 39 | 68 | 28 | 119 | 153 | 132 |
Endoplasmic Reticulum | 7 | 3 | 2 | 1 | 3 | 1 | 1 | 1 | 4 | 0 | 0 | 2 | 1 | 0 | 1 | 1 | – | – | 3 | 1 | 11 | 2 | 2 | 5 |
Endosome | 1 | 8 | 7 | 1 | 8 | 0 | 0 | 4 | 0 | 0 | 1 | 0 | 8 | 4 | 10 | 0 | – | – | 17 | 17 | 27 | 19 | 26 | 33 |
Golgi | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 1 | 0 | 4 | 0 | 2 | 3 |
Mitochondria | 99 | 67 | 40 | 81 | 97 | 283 | 324 | 271 | 290 | 240 | 319 | 297 | 3 | 0 | 2 | 5 | – | – | 143 | 120 | 240 | 205 | 263 | 289 |
Nucleus | 0 | 0 | 0 | 0 | 3 | 2 | 3 | 5 | 7 | 5 | 5 | 3 | 0 | 3 | 0 | 1 | – | – | 0 | 0 | 0 | 2 | 3 | 4 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | – | 0 | 0 | 0 | 2 | 0 | 2 |
Nucleolus | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 2 | 1 | 2 |
Peroxisomes | 0 | 0 | 8 | 2 | 1 | 1 | 2 | 2 | 0 | 0 | 0 | 1 | 2 | 3 | 3 | 0 | – | – | 1 | 0 | 11 | 2 | 2 | 3 |
SpindlePole | 5 | 3 | 31 | 16 | 10 | 14 | 29 | 34 | 25 | 23 | 14 | 26 | 4 | 2 | 1 | 0 | – | – | 9 | 9 | 18 | 18 | 21 | 26 |
Vac/Vac Membrane | 5 | 2 | 3 | 1 | 4 | 10 | 4 | 9 | 5 | 4 | 6 | 11 | 6 | 3 | 4 | 0 | – | – | 15 | 15 | 7 | 25 | 32 | 27 |
Unique Cell Count | 194 | 214 | 272 | 179 | 214 | 399 | 438 | 398 | 374 | 289 | 374 | 345 | 503 | 365 | 494 | 34 | 248 | 255 | 382 | 438 | 566 | 607 | ||
Labelled Cell Count | 228 | 238 | 296 | 212 | 251 | 484 | 549 | 492 | 462 | 370 | 468 | 436 | 513 | 382 | 504 | 39 | 248 | 255 | 382 | 438 | 566 | 607 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.2 | 3.7 | 4.0 | 3.5 | 4.1 | 3.2 | 3.1 | 3.3 | 3.0 | 2.9 | 2.4 | 2.6 | 4.9 | 5.0 | 4.9 | 4.9 | 4.8 | 4.7 | 5.1 | 5.1 | 5.7 |
Std Deviation (1e-4) | 0.4 | 0.6 | 1.2 | 1.3 | 2.0 | 1.8 | 1.6 | 1.6 | 1.3 | 1.1 | 1.0 | 1.0 | 0.9 | 0.8 | 1.1 | 1.3 | 2.3 | 1.1 | 1.2 | 1.1 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.19 | 0.03 | -0.32 | -0.37 | -0.31 | -0.43 | -0.5 | -0.73 | -0.63 | 0.28 | 0.31 | 0.26 | 0.28 | 0.25 | 0.21 | 0.34 | 0.33 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2931 | 2.0732 | 1.6591 | 2.0235 | 1.8314 | 1.9634 | 0.2944 | 0.0719 | 0.1494 | -0.1081 | -0.2514 | 0.1889 | -1.4366 | -0.9514 | -1.2461 | -0.7502 | -1.1374 | -1.0243 |
Actin | 0.0104 | 0.0075 | 0.0062 | 0.0016 | 0.0335 | 0.005 | 0.0306 | 0.011 | 0.0164 | 0.0061 | 0.0296 | 0.017 | 0.0275 | 0.0113 | 0.0088 | 0.0042 | 0.0477 | 0.0186 |
Bud | 0.0008 | 0.0115 | 0.0052 | 0.0037 | 0.0006 | 0.0027 | 0.0031 | 0.006 | 0.012 | 0.0037 | 0.0025 | 0.0027 | 0.0039 | 0.0027 | 0.0021 | 0.002 | 0.0009 | 0.007 |
Bud Neck | 0.0034 | 0.0039 | 0.0176 | 0.0018 | 0.0398 | 0.0136 | 0.017 | 0.0011 | 0.0066 | 0.0167 | 0.0023 | 0.0104 | 0.008 | 0.0021 | 0.0027 | 0.0111 | 0.0008 | 0.0078 |
Bud Periphery | 0.0028 | 0.0083 | 0.0105 | 0.0115 | 0.0013 | 0.0031 | 0.0105 | 0.0112 | 0.0256 | 0.0152 | 0.0088 | 0.0098 | 0.0096 | 0.0057 | 0.0049 | 0.0042 | 0.002 | 0.0085 |
Bud Site | 0.0058 | 0.085 | 0.0167 | 0.0017 | 0.0068 | 0.0015 | 0.0127 | 0.0252 | 0.031 | 0.0104 | 0.0026 | 0.0021 | 0.0147 | 0.0249 | 0.0151 | 0.0039 | 0.0011 | 0.0013 |
Cell Periphery | 0.0029 | 0.0031 | 0.0035 | 0.0006 | 0.0005 | 0.0014 | 0.0014 | 0.0017 | 0.0029 | 0.0017 | 0.0007 | 0.0021 | 0.0023 | 0.0016 | 0.0018 | 0.0008 | 0.0004 | 0.0007 |
Cytoplasm | 0.111 | 0.1672 | 0.1221 | 0.1006 | 0.0736 | 0.153 | 0.0988 | 0.1445 | 0.131 | 0.1093 | 0.1352 | 0.1626 | 0.1008 | 0.1588 | 0.1404 | 0.1107 | 0.1614 | 0.1852 |
Cytoplasmic Foci | 0.1068 | 0.1105 | 0.1001 | 0.0939 | 0.0772 | 0.1085 | 0.129 | 0.1241 | 0.1323 | 0.1768 | 0.1665 | 0.1507 | 0.185 | 0.1269 | 0.1066 | 0.1185 | 0.122 | 0.1631 |
Eisosomes | 0.0021 | 0.0007 | 0.0014 | 0.0004 | 0.0006 | 0.0006 | 0.0006 | 0.001 | 0.0012 | 0.0008 | 0.0003 | 0.0004 | 0.0012 | 0.0012 | 0.0018 | 0.0002 | 0.0008 | 0.0005 |
Endoplasmic Reticulum | 0.0067 | 0.0049 | 0.0026 | 0.0038 | 0.0063 | 0.0038 | 0.0082 | 0.0049 | 0.0053 | 0.0062 | 0.0043 | 0.0056 | 0.0034 | 0.0043 | 0.0054 | 0.003 | 0.0045 | 0.0034 |
Endosome | 0.0572 | 0.0512 | 0.0421 | 0.0805 | 0.0472 | 0.0486 | 0.1436 | 0.0767 | 0.0832 | 0.1165 | 0.1124 | 0.0696 | 0.1072 | 0.1025 | 0.0806 | 0.084 | 0.0918 | 0.0864 |
Golgi | 0.0114 | 0.0116 | 0.0127 | 0.0132 | 0.0136 | 0.0116 | 0.0215 | 0.0209 | 0.0236 | 0.0196 | 0.033 | 0.0164 | 0.0287 | 0.0168 | 0.0248 | 0.0126 | 0.0288 | 0.0199 |
Lipid Particles | 0.0223 | 0.0067 | 0.0123 | 0.0059 | 0.0085 | 0.0181 | 0.0321 | 0.0102 | 0.0209 | 0.0073 | 0.0123 | 0.0126 | 0.0242 | 0.0064 | 0.0084 | 0.0152 | 0.0163 | 0.0135 |
Mitochondria | 0.0361 | 0.0289 | 0.0819 | 0.0728 | 0.0389 | 0.0726 | 0.0656 | 0.0694 | 0.1235 | 0.1097 | 0.1609 | 0.0934 | 0.0584 | 0.0373 | 0.1012 | 0.091 | 0.0708 | 0.0781 |
None | 0.5811 | 0.4584 | 0.5187 | 0.5715 | 0.6087 | 0.5166 | 0.359 | 0.4458 | 0.3319 | 0.345 | 0.2763 | 0.3851 | 0.365 | 0.4559 | 0.4669 | 0.4848 | 0.3976 | 0.3628 |
Nuclear Periphery | 0.0018 | 0.0018 | 0.0032 | 0.002 | 0.0007 | 0.0013 | 0.0119 | 0.003 | 0.0028 | 0.0053 | 0.0018 | 0.0078 | 0.0013 | 0.0011 | 0.0017 | 0.002 | 0.0027 | 0.0028 |
Nucleolus | 0.0034 | 0.0018 | 0.0019 | 0.0011 | 0.0004 | 0.0016 | 0.0046 | 0.0019 | 0.002 | 0.0014 | 0.0016 | 0.0013 | 0.0037 | 0.0014 | 0.0007 | 0.0011 | 0.0008 | 0.0006 |
Nucleus | 0.0029 | 0.0039 | 0.0033 | 0.0025 | 0.0014 | 0.0022 | 0.0066 | 0.0036 | 0.0041 | 0.0025 | 0.0025 | 0.0044 | 0.0025 | 0.0017 | 0.0015 | 0.0033 | 0.0016 | 0.0058 |
Peroxisomes | 0.0136 | 0.0128 | 0.0252 | 0.0112 | 0.0368 | 0.0229 | 0.0152 | 0.022 | 0.0254 | 0.0218 | 0.0207 | 0.028 | 0.0371 | 0.0228 | 0.0144 | 0.0127 | 0.0361 | 0.0202 |
Punctate Nuclear | 0.0067 | 0.0044 | 0.0051 | 0.0075 | 0.0014 | 0.0041 | 0.0129 | 0.004 | 0.0045 | 0.0025 | 0.0036 | 0.008 | 0.0037 | 0.0013 | 0.0012 | 0.0048 | 0.0018 | 0.0068 |
Vacuole | 0.0089 | 0.0137 | 0.0064 | 0.0091 | 0.0015 | 0.0055 | 0.012 | 0.0096 | 0.0104 | 0.0139 | 0.0163 | 0.0067 | 0.0096 | 0.0112 | 0.0068 | 0.0239 | 0.0073 | 0.005 |
Vacuole Periphery | 0.0017 | 0.0023 | 0.0015 | 0.003 | 0.0007 | 0.0016 | 0.003 | 0.0021 | 0.0032 | 0.0078 | 0.006 | 0.0033 | 0.0022 | 0.0022 | 0.0022 | 0.0058 | 0.0028 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | – |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|