Standard name
Human Ortholog
Description GPI-anchored aspartyl protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p; MKC7 has a paralog, YPS1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0.05 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.1 0.29 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.89 0.93 – 0.95 0.99 0.97 0.96 – 0.91 0.88 0.87 0.82 – – – 0.79 0.76 0.74 0.3 0.35 0.21 0.39 0.1 0.33
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.11 0 0.06
Endosome 0 0 – 0.05 0 0 0 – 0 0 0 0 – – – 0 0 0 0.06 0 0 0.16 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0.06 0
Mitochondria 0.05 0 – 0.05 0 0 0 – 0.06 0.14 0.18 0.22 – – – 0 0 0 0 0 0.07 0 0 0
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.13
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.1 – 0 0 0 0 – 0 0 0 0 – – – 0.26 0.35 0.32 0.54 0.54 0.62 0.2 0.23 0.36
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 1 – – – 0 0 0 0 0 0 0 0 0
Bud 2 0 – 1 0 0 0 – 2 0 1 2 – – – 0 0 0 0 1 0 0 1 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 1 1 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 1 0 1 – 0 0 2 3 – – – 0 1 0 0 0 0 0 0 0
Cytoplasm 119 190 – 20 94 104 161 – 43 49 89 67 – – – 219 179 170 44 77 9 1 0 7
Endoplasmic Reticulum 1 1 – 0 0 1 0 – 0 2 1 0 – – – 5 10 8 3 0 0 0 0 1
Endosome 2 2 – 1 0 0 1 – 0 0 0 0 – – – 7 5 10 9 5 1 0 0 0
Golgi 0 0 – 0 1 0 0 – 0 0 0 0 – – – 1 2 0 0 5 0 0 0 0
Mitochondria 7 1 – 1 0 2 1 – 3 8 18 18 – – – 1 3 3 4 4 3 0 0 0
Nucleus 0 1 – 0 0 1 1 – 0 0 0 3 – – – 8 3 4 0 0 0 0 0 2
Nuclear Periphery 0 1 – 0 0 1 0 – 1 1 1 1 – – – 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 1 0 1 – – – 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 1 0 – – – 0 0 2 1 0 0 0 0 0
Vac/Vac Membrane 12 20 – 0 1 3 6 – 1 1 1 2 – – – 73 82 74 81 120 28 0 1 7
Unique Cell Count 134 205 21 95 107 167 47 56 102 82 276 235 231 150 221 46 5 7 22
Labelled Cell Count 143 216 23 97 112 171 50 62 114 98 314 287 271 150 221 46 5 7 22


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.0 – 4.5 5.8 4.9 5.0 – 4.0 3.9 4.0 4.8 – – – 6.7 6.8 6.3 – – –
Std Deviation (1e-4) 2.5 1.1 – 0.7 1.3 1.6 1.6 – 1.5 0.6 1.6 8.3 – – – 1.5 1.8 1.4 – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0772 0.3753 0.5674 0.5191 0.3303 0.4677 -0.153 0.2182 -0.1564 0.1114 -0.4 -0.1686 -0.3664 -0.1456 -0.1287 -0.2195 -0.163 -0.079
Actin 0.0211 0.0001 0.0029 0.0134 0.0214 0.0003 0.0384 0.009 0.0077 0.0072 0.0111 0.0004 0.044 0.0001 0.006 0.0063 0.0222 0.0023
Bud 0.001 0.0015 0.001 0.0025 0.0007 0.0012 0.0031 0.0009 0.0015 0.0009 0.0008 0.0007 0.0124 0.0034 0.0147 0.0009 0.0017 0.001
Bud Neck 0.0009 0.0014 0.0094 0.0063 0.0042 0.0028 0.0024 0.0064 0.0007 0.0091 0.0018 0.0054 0.0022 0.0014 0.0009 0.006 0.0014 0.0025
Bud Periphery 0.0024 0.0018 0.0025 0.0022 0.0014 0.0014 0.0118 0.0021 0.0033 0.0031 0.0015 0.0021 0.0085 0.0012 0.0047 0.0015 0.0033 0.0009
Bud Site 0.0021 0.0045 0.0142 0.0142 0.0088 0.0006 0.0119 0.012 0.0033 0.0071 0.0031 0.0017 0.0063 0.0085 0.0075 0.01 0.0077 0.0006
Cell Periphery 0.0009 0.0014 0.0012 0.0006 0.0008 0.0009 0.0017 0.0024 0.0009 0.0011 0.001 0.0014 0.0018 0.0011 0.0012 0.0007 0.0007 0.0005
Cytoplasm 0.0707 0.1238 0.1183 0.0443 0.0465 0.1043 0.0975 0.1921 0.1373 0.1028 0.0396 0.1264 0.1468 0.2603 0.2127 0.0728 0.0802 0.1817
Cytoplasmic Foci 0.0909 0.0451 0.0633 0.0829 0.0789 0.0638 0.0739 0.0448 0.0502 0.0807 0.0816 0.0526 0.0668 0.0349 0.0514 0.103 0.054 0.0714
Eisosomes 0.0006 0.0002 0.0003 0.0001 0.0004 0.0001 0.0008 0.0001 0.0002 0.0002 0.0001 0 0.0023 0.0001 0.0001 0.0003 0.0004 0.0001
Endoplasmic Reticulum 0.0125 0.0075 0.0064 0.0018 0.0028 0.0039 0.0086 0.007 0.0059 0.0058 0.0054 0.007 0.0127 0.0062 0.0055 0.0046 0.0046 0.0043
Endosome 0.3032 0.209 0.2863 0.3031 0.2227 0.2316 0.1244 0.0956 0.1584 0.2446 0.3355 0.21 0.1615 0.1336 0.1996 0.3044 0.2598 0.1774
Golgi 0.0212 0.0033 0.0086 0.0309 0.0427 0.0069 0.0152 0.0029 0.0045 0.0115 0.0139 0.0027 0.0067 0.0018 0.0054 0.0054 0.0198 0.0025
Lipid Particles 0.0334 0.0141 0.0135 0.0152 0.0308 0.016 0.0321 0.0097 0.0145 0.0376 0.0488 0.0103 0.0145 0.0037 0.0167 0.0194 0.0219 0.0115
Mitochondria 0.0238 0.0112 0.0281 0.0205 0.0384 0.0218 0.0327 0.0129 0.0133 0.0277 0.0124 0.0066 0.0129 0.0019 0.0048 0.0119 0.0213 0.0089
None 0.1752 0.152 0.2025 0.0429 0.203 0.1149 0.3848 0.2893 0.3324 0.2095 0.1116 0.195 0.2875 0.186 0.2105 0.0787 0.2093 0.1635
Nuclear Periphery 0.0216 0.0075 0.0057 0.0044 0.0034 0.0071 0.0089 0.0045 0.0082 0.0061 0.0043 0.0063 0.0118 0.0041 0.0158 0.0077 0.0067 0.0046
Nucleolus 0.0021 0.0043 0.0044 0.0036 0.0026 0.0036 0.0021 0.0057 0.0038 0.0068 0.018 0.0034 0.0026 0.003 0.004 0.0041 0.0043 0.0017
Nucleus 0.0067 0.0105 0.0066 0.0065 0.0045 0.0269 0.0056 0.0113 0.0106 0.0197 0.0173 0.0135 0.008 0.011 0.0105 0.007 0.0068 0.0186
Peroxisomes 0.0071 0.0019 0.0043 0.0092 0.007 0.0035 0.0239 0.0062 0.0133 0.0428 0.0195 0.0013 0.0079 0.0004 0.0059 0.01 0.0061 0.0156
Punctate Nuclear 0.0039 0.0013 0.0028 0.009 0.0037 0.0073 0.0111 0.0022 0.0046 0.0119 0.0041 0.0028 0.0085 0.0011 0.006 0.016 0.0045 0.0015
Vacuole 0.1787 0.3627 0.1935 0.3264 0.2434 0.3418 0.0966 0.2645 0.1997 0.1452 0.2525 0.3288 0.1587 0.3186 0.1996 0.281 0.2468 0.3064
Vacuole Periphery 0.02 0.0348 0.0242 0.06 0.032 0.0393 0.0126 0.0184 0.0257 0.0184 0.016 0.0216 0.0157 0.0177 0.0164 0.0483 0.0164 0.0225

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 99.1893 64.9874 42.0933 60.5525 75.4846 59.727 56.9382 58.6308 46.8881 88.5342
Translational Efficiency 0.8072 0.7323 0.8799 0.5603 0.62 1.2215 0.7749 0.4647 0.696 0.6332

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1607 1079 1284 1447 1425 1579 1762 915 3032 2658 3046 2362

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 580.92 697.34 876.82 838.46 690.74 705.73 848.50 860.78 632.53 702.32 860.44 847.11
Standard Deviation 62.31 108.25 103.32 108.13 75.82 92.45 101.11 112.28 88.11 99.26 103.00 110.29
Intensity Change Log 2 — 0.263523 0.593941 0.529402 — 0.030974 0.296772 0.317502 — 0.141874 0.440151 0.418177

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000247 0.000477 0.000202 0.001578 0.000072 0.000129 0.000122 0.000799 0.000165 0.000270 0.000156 0.001276
Bud Neck 0.042912 0.043652 0.003974 0.006561 0.015983 0.045730 0.002123 0.004372 0.030256 0.044887 0.002903 0.005713
Bud Site 0.010687 0.022218 0.011422 0.035024 0.003785 0.015315 0.003456 0.035122 0.007443 0.018117 0.006814 0.035062
Cell Periphery 0.000395 0.000143 0.000123 0.000215 0.000139 0.000223 0.000099 0.000166 0.000275 0.000191 0.000109 0.000196
Cytoplasm 0.449657* 0.287650* 0.372509* 0.510084* 0.498877* 0.320504* 0.512067* 0.466523* 0.472790* 0.307167* 0.453238* 0.493209*
Cytoplasmic Foci 0.228072* 0.279211* 0.015127 0.035811 0.172913 0.141299 0.010917 0.038587 0.202148* 0.197283* 0.012692 0.036887
Eisosomes 0.000732 0.000188 0.000047 0.000066 0.000138 0.000120 0.000029 0.000060 0.000453 0.000148 0.000037 0.000064
Endoplasmic Reticulum 0.001377 0.001179 0.002909 0.002971 0.000779 0.001986 0.001628 0.002180 0.001096 0.001658 0.002168 0.002664
Endosome 0.017142 0.085027 0.003349 0.006606 0.018141 0.055542 0.002113 0.013427 0.017611 0.067511 0.002634 0.009248
Golgi 0.004329 0.006977 0.000071 0.003844 0.000992 0.004230 0.000100 0.007922 0.002761 0.005345 0.000088 0.005424
Lipid Particles 0.015220 0.009420 0.002013 0.002450 0.005390 0.010213 0.000885 0.002700 0.010600 0.009891 0.001360 0.002547
Mitochondria 0.005227 0.013183 0.000633 0.001266 0.001141 0.008119 0.000560 0.005698 0.003307 0.010175 0.000591 0.002983
Mitotic Spindle 0.000290 0.003028 0.004496 0.019857 0.001377 0.011294 0.003563 0.043981 0.000801 0.007938 0.003956 0.029202
None 0.009801 0.002249 0.005798 0.003027 0.002435 0.004035 0.005782 0.004577 0.006339 0.003310 0.005789 0.003628
Nuclear Periphery 0.000314 0.000450 0.002363 0.001384 0.000466 0.000774 0.001552 0.001330 0.000385 0.000643 0.001894 0.001363
Nuclear Periphery Foci 0.000594 0.000556 0.001085 0.002825 0.000278 0.000342 0.000990 0.003166 0.000445 0.000429 0.001030 0.002957
Nucleolus 0.002129 0.002841 0.000793 0.000328 0.000833 0.002932 0.000433 0.000353 0.001520 0.002895 0.000585 0.000338
Nucleus 0.131772* 0.043070 0.495814* 0.157977* 0.138779* 0.119814 0.386853* 0.152099* 0.135065* 0.088660 0.432784* 0.155700*
Peroxisomes 0.006306 0.021556 0.000410 0.000928 0.003034 0.007241 0.000832 0.004335 0.004768 0.013052 0.000654 0.002248
Vacuole 0.071701 0.172431 0.076529 0.206417* 0.133687 0.245325* 0.065540 0.207133* 0.100833 0.215734* 0.070172 0.206694*
Vacuole Periphery 0.001095 0.004494 0.000333 0.000781 0.000762 0.004833 0.000356 0.005469 0.000938 0.004695 0.000347 0.002597

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.05 -0.96 -2.61 -1.62 -2.36 -2.85 -4.16 -5.99 -5.18 -4.79 -3.84 -1.95 -3.82 -3.09 -3.45
Bud Neck -0.00 16.33 15.39 14.01 -2.59 -9.99 9.32 6.99 15.06 -5.55 -6.11 18.74 16.52 20.16 -5.82
Bud Site -5.33 0.55 -3.46 0.77 -3.48 -7.85 0.81 -5.93 -1.56 -6.30 -8.37 1.55 -6.53 -0.86 -7.25
Cell Periphery 10.93 12.31 9.14 -1.72 -3.25 -4.50 4.83 1.23 5.45 -4.42 5.31 13.30 8.38 2.81 -5.20
Cytoplasm 16.42 14.07 4.60 -10.85 -8.55 18.17 7.29 11.36 -3.91 5.50 23.85 13.47 11.07 -10.55 -1.17
Cytoplasmic Foci -6.13 43.29 38.79 38.56 -9.14 5.20 35.44 29.49 24.95 -10.16 1.23 55.59 48.16 42.44 -14.09
Eisosomes 9.13 11.43 11.20 10.86 -3.49 2.61 18.45 12.17 8.92 -10.52 9.52 12.76 12.02 13.80 -9.83
Endoplasmic Reticulum 0.79 -10.95 -9.01 -8.55 3.47 -5.31 -11.83 -11.29 -3.98 -1.72 -3.08 -14.87 -14.07 -8.68 0.69
Endosome -16.19 12.67 11.06 19.79 -1.91 -13.41 12.95 7.01 18.14 -6.22 -20.99 18.34 13.04 27.23 -6.63
Golgi -3.17 13.40 4.15 5.65 -2.18 -5.46 7.73 -1.74 1.63 -2.81 -5.16 14.92 1.70 5.04 -3.49
Lipid Particles 5.01 13.58 13.19 9.31 -1.61 -5.19 12.13 6.64 8.57 -6.57 1.02 16.73 14.54 12.15 -6.89
Mitochondria -4.26 9.20 8.79 6.97 -0.69 -7.31 3.08 -1.07 4.58 -1.79 -7.16 9.65 4.44 9.05 -1.99
Mitotic Spindle -3.65 -3.41 -6.59 -5.09 -4.19 -5.11 -2.40 -7.06 -4.52 -6.43 -6.08 -4.30 -9.52 -5.63 -7.71
None 4.66 3.39 5.05 -0.62 2.70 -1.66 -3.08 -2.62 -0.19 0.32 3.07 2.10 3.72 -0.05 2.66
Nuclear Periphery -0.97 -22.80 -14.33 -10.10 3.71 -4.18 -22.99 -16.08 -13.38 -1.80 -3.61 -32.67 -20.42 -15.76 1.52
Nuclear Periphery Foci 0.27 -5.64 -9.07 -9.26 -6.44 -1.31 -10.71 -9.38 -9.14 -7.30 0.10 -9.66 -13.15 -13.24 -9.92
Nucleolus -0.79 3.27 3.81 2.73 1.80 -5.64 4.57 1.26 5.96 -2.60 -3.05 4.44 4.02 5.61 -0.58
Nucleus 20.18 -43.37 -7.32 -24.32 36.37 3.30 -36.43 -3.21 -5.63 27.79 11.89 -55.74 -7.59 -17.07 44.96
Peroxisomes -9.55 9.91 10.01 14.02 -0.84 -4.84 5.00 1.75 5.43 -1.51 -9.75 10.61 8.31 14.70 -1.65
Vacuole -16.83 -7.56 -31.42 -15.57 -23.18 -14.23 1.76 -19.44 -8.70 -20.55 -22.54 -4.20 -35.15 -15.44 -30.95
Vacuole Periphery -7.46 8.22 4.63 8.94 -0.33 -11.67 4.67 -1.57 4.11 -2.37 -13.48 8.35 0.12 9.89 -2.38
Standard name
Human Ortholog
Description GPI-anchored aspartyl protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p; MKC7 has a paralog, YPS1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (21%), vacuole (11%), mixed (45%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.00046)
Subcompartmental Group N/A

Mkc7

Mkc7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mkc7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available