Standard name | |
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Human Ortholog | |
Description | GPI-anchored aspartyl protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p; MKC7 has a paralog, YPS1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 2 | 0 | – | 1 | 0 | 0 | 0 | – | 2 | 0 | 1 | 2 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 0 | 1 | 0 | 1 | – | 0 | 0 | 2 | 3 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 119 | 190 | – | 20 | 94 | 104 | 161 | – | 43 | 49 | 89 | 67 | – | – | – | 219 | 179 | 170 | 44 | 77 | 9 | 1 | 0 | 7 |
Endoplasmic Reticulum | 1 | 1 | – | 0 | 0 | 1 | 0 | – | 0 | 2 | 1 | 0 | – | – | – | 5 | 10 | 8 | 3 | 0 | 0 | 0 | 0 | 1 |
Endosome | 2 | 2 | – | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | – | – | – | 7 | 5 | 10 | 9 | 5 | 1 | 0 | 0 | 0 |
Golgi | 0 | 0 | – | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 1 | 2 | 0 | 0 | 5 | 0 | 0 | 0 | 0 |
Mitochondria | 7 | 1 | – | 1 | 0 | 2 | 1 | – | 3 | 8 | 18 | 18 | – | – | – | 1 | 3 | 3 | 4 | 4 | 3 | 0 | 0 | 0 |
Nucleus | 0 | 1 | – | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 3 | – | – | – | 8 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 2 |
Nuclear Periphery | 0 | 1 | – | 0 | 0 | 1 | 0 | – | 1 | 1 | 1 | 1 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | – | – | – | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 12 | 20 | – | 0 | 1 | 3 | 6 | – | 1 | 1 | 1 | 2 | – | – | – | 73 | 82 | 74 | 81 | 120 | 28 | 0 | 1 | 7 |
Unique Cell Count | 134 | 205 | 21 | 95 | 107 | 167 | 47 | 56 | 102 | 82 | 276 | 235 | 231 | 150 | 221 | 46 | 5 | 7 | 22 | |||||
Labelled Cell Count | 143 | 216 | 23 | 97 | 112 | 171 | 50 | 62 | 114 | 98 | 314 | 287 | 271 | 150 | 221 | 46 | 5 | 7 | 22 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 5.0 | – | 4.5 | 5.8 | 4.9 | 5.0 | – | 4.0 | 3.9 | 4.0 | 4.8 | – | – | – | 6.7 | 6.8 | 6.3 | – | – | – |
Std Deviation (1e-4) | 2.5 | 1.1 | – | 0.7 | 1.3 | 1.6 | 1.6 | – | 1.5 | 0.6 | 1.6 | 8.3 | – | – | – | 1.5 | 1.8 | 1.4 | – | – | – |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0772 | 0.3753 | 0.5674 | 0.5191 | 0.3303 | 0.4677 | -0.153 | 0.2182 | -0.1564 | 0.1114 | -0.4 | -0.1686 | -0.3664 | -0.1456 | -0.1287 | -0.2195 | -0.163 | -0.079 |
Actin | 0.0211 | 0.0001 | 0.0029 | 0.0134 | 0.0214 | 0.0003 | 0.0384 | 0.009 | 0.0077 | 0.0072 | 0.0111 | 0.0004 | 0.044 | 0.0001 | 0.006 | 0.0063 | 0.0222 | 0.0023 |
Bud | 0.001 | 0.0015 | 0.001 | 0.0025 | 0.0007 | 0.0012 | 0.0031 | 0.0009 | 0.0015 | 0.0009 | 0.0008 | 0.0007 | 0.0124 | 0.0034 | 0.0147 | 0.0009 | 0.0017 | 0.001 |
Bud Neck | 0.0009 | 0.0014 | 0.0094 | 0.0063 | 0.0042 | 0.0028 | 0.0024 | 0.0064 | 0.0007 | 0.0091 | 0.0018 | 0.0054 | 0.0022 | 0.0014 | 0.0009 | 0.006 | 0.0014 | 0.0025 |
Bud Periphery | 0.0024 | 0.0018 | 0.0025 | 0.0022 | 0.0014 | 0.0014 | 0.0118 | 0.0021 | 0.0033 | 0.0031 | 0.0015 | 0.0021 | 0.0085 | 0.0012 | 0.0047 | 0.0015 | 0.0033 | 0.0009 |
Bud Site | 0.0021 | 0.0045 | 0.0142 | 0.0142 | 0.0088 | 0.0006 | 0.0119 | 0.012 | 0.0033 | 0.0071 | 0.0031 | 0.0017 | 0.0063 | 0.0085 | 0.0075 | 0.01 | 0.0077 | 0.0006 |
Cell Periphery | 0.0009 | 0.0014 | 0.0012 | 0.0006 | 0.0008 | 0.0009 | 0.0017 | 0.0024 | 0.0009 | 0.0011 | 0.001 | 0.0014 | 0.0018 | 0.0011 | 0.0012 | 0.0007 | 0.0007 | 0.0005 |
Cytoplasm | 0.0707 | 0.1238 | 0.1183 | 0.0443 | 0.0465 | 0.1043 | 0.0975 | 0.1921 | 0.1373 | 0.1028 | 0.0396 | 0.1264 | 0.1468 | 0.2603 | 0.2127 | 0.0728 | 0.0802 | 0.1817 |
Cytoplasmic Foci | 0.0909 | 0.0451 | 0.0633 | 0.0829 | 0.0789 | 0.0638 | 0.0739 | 0.0448 | 0.0502 | 0.0807 | 0.0816 | 0.0526 | 0.0668 | 0.0349 | 0.0514 | 0.103 | 0.054 | 0.0714 |
Eisosomes | 0.0006 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0023 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0001 |
Endoplasmic Reticulum | 0.0125 | 0.0075 | 0.0064 | 0.0018 | 0.0028 | 0.0039 | 0.0086 | 0.007 | 0.0059 | 0.0058 | 0.0054 | 0.007 | 0.0127 | 0.0062 | 0.0055 | 0.0046 | 0.0046 | 0.0043 |
Endosome | 0.3032 | 0.209 | 0.2863 | 0.3031 | 0.2227 | 0.2316 | 0.1244 | 0.0956 | 0.1584 | 0.2446 | 0.3355 | 0.21 | 0.1615 | 0.1336 | 0.1996 | 0.3044 | 0.2598 | 0.1774 |
Golgi | 0.0212 | 0.0033 | 0.0086 | 0.0309 | 0.0427 | 0.0069 | 0.0152 | 0.0029 | 0.0045 | 0.0115 | 0.0139 | 0.0027 | 0.0067 | 0.0018 | 0.0054 | 0.0054 | 0.0198 | 0.0025 |
Lipid Particles | 0.0334 | 0.0141 | 0.0135 | 0.0152 | 0.0308 | 0.016 | 0.0321 | 0.0097 | 0.0145 | 0.0376 | 0.0488 | 0.0103 | 0.0145 | 0.0037 | 0.0167 | 0.0194 | 0.0219 | 0.0115 |
Mitochondria | 0.0238 | 0.0112 | 0.0281 | 0.0205 | 0.0384 | 0.0218 | 0.0327 | 0.0129 | 0.0133 | 0.0277 | 0.0124 | 0.0066 | 0.0129 | 0.0019 | 0.0048 | 0.0119 | 0.0213 | 0.0089 |
None | 0.1752 | 0.152 | 0.2025 | 0.0429 | 0.203 | 0.1149 | 0.3848 | 0.2893 | 0.3324 | 0.2095 | 0.1116 | 0.195 | 0.2875 | 0.186 | 0.2105 | 0.0787 | 0.2093 | 0.1635 |
Nuclear Periphery | 0.0216 | 0.0075 | 0.0057 | 0.0044 | 0.0034 | 0.0071 | 0.0089 | 0.0045 | 0.0082 | 0.0061 | 0.0043 | 0.0063 | 0.0118 | 0.0041 | 0.0158 | 0.0077 | 0.0067 | 0.0046 |
Nucleolus | 0.0021 | 0.0043 | 0.0044 | 0.0036 | 0.0026 | 0.0036 | 0.0021 | 0.0057 | 0.0038 | 0.0068 | 0.018 | 0.0034 | 0.0026 | 0.003 | 0.004 | 0.0041 | 0.0043 | 0.0017 |
Nucleus | 0.0067 | 0.0105 | 0.0066 | 0.0065 | 0.0045 | 0.0269 | 0.0056 | 0.0113 | 0.0106 | 0.0197 | 0.0173 | 0.0135 | 0.008 | 0.011 | 0.0105 | 0.007 | 0.0068 | 0.0186 |
Peroxisomes | 0.0071 | 0.0019 | 0.0043 | 0.0092 | 0.007 | 0.0035 | 0.0239 | 0.0062 | 0.0133 | 0.0428 | 0.0195 | 0.0013 | 0.0079 | 0.0004 | 0.0059 | 0.01 | 0.0061 | 0.0156 |
Punctate Nuclear | 0.0039 | 0.0013 | 0.0028 | 0.009 | 0.0037 | 0.0073 | 0.0111 | 0.0022 | 0.0046 | 0.0119 | 0.0041 | 0.0028 | 0.0085 | 0.0011 | 0.006 | 0.016 | 0.0045 | 0.0015 |
Vacuole | 0.1787 | 0.3627 | 0.1935 | 0.3264 | 0.2434 | 0.3418 | 0.0966 | 0.2645 | 0.1997 | 0.1452 | 0.2525 | 0.3288 | 0.1587 | 0.3186 | 0.1996 | 0.281 | 0.2468 | 0.3064 |
Vacuole Periphery | 0.02 | 0.0348 | 0.0242 | 0.06 | 0.032 | 0.0393 | 0.0126 | 0.0184 | 0.0257 | 0.0184 | 0.016 | 0.0216 | 0.0157 | 0.0177 | 0.0164 | 0.0483 | 0.0164 | 0.0225 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 99.1893 | 64.9874 | 42.0933 | 60.5525 | 75.4846 | 59.727 | 56.9382 | 58.6308 | 46.8881 | 88.5342 |
Translational Efficiency | 0.8072 | 0.7323 | 0.8799 | 0.5603 | 0.62 | 1.2215 | 0.7749 | 0.4647 | 0.696 | 0.6332 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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