Standard name
Human Ortholog
Description Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.96 0.97 0.95 0.96 0.81 0.87 0.85 0.8 0.77 0.79 0.99 1.0 0.98 0.97 0.94 0.93 0.9 0.91 0.92 0.96 0.93 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0.11 0.09 0.18 0.4 0.31 0.39 0.45 0.52 0.5 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 2
Bud 0 0 2 1 1 1 0 5 3 8 2 4 1 0 1 0 0 0 1 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 5 0 0 0 1 0 0 0 0 0 0 1 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 1 0 1 2 0 1 2 1 0 0 1 2 2 3 5 0 0 0 0 0 0
Cytoplasm 266 246 163 156 229 155 155 294 185 257 86 100 293 422 395 362 220 141 258 230 159 185 224 211
Endoplasmic Reticulum 0 2 1 0 0 0 0 0 0 0 0 0 2 0 2 15 15 5 3 0 0 0 2 1
Endosome 0 0 0 1 1 0 0 0 0 0 0 0 0 0 1 4 3 3 3 3 0 1 3 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 1
Mitochondria 15 4 6 17 22 29 77 106 85 143 58 63 0 0 2 12 4 2 4 3 5 0 0 0
Nucleus 0 0 2 0 1 1 1 3 1 1 1 0 1 1 1 4 2 3 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 2 0 1 0 0 0 1 0
Vac/Vac Membrane 2 0 0 1 1 0 0 0 1 4 0 0 0 1 2 6 8 5 2 7 2 0 1 2
Unique Cell Count 275 252 170 161 241 162 192 337 217 321 112 126 295 424 403 372 233 151 288 253 174 193 242 228
Labelled Cell Count 283 253 177 177 259 187 235 415 277 416 149 169 297 425 406 405 257 164 288 253 174 193 242 228


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.0 3.9 3.8 4.1 3.4 2.5 3.0 3.0 2.7 2.9 2.8 5.2 4.9 4.9 5.4 6.8 6.9 4.9 5.4 5.6
Std Deviation (1e-4) 0.7 0.8 0.7 1.3 1.4 1.6 0.4 0.9 0.9 0.9 1.0 1.2 1.0 1.0 1.3 1.6 1.9 1.9 1.0 1.8 1.0
Intensity Change (Log2) – – – -0.05 0.05 -0.2 -0.67 -0.38 -0.41 -0.54 -0.46 -0.49 0.39 0.32 0.3 0.46 0.79 0.8 0.32 0.45 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 -0.4 -0.1 -4.4 -3.1 -3.4 -4.7 -4.9 -4.5 2.6 3.3 1.5 0.9 -0.7 -1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.2 3.0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 -0.2 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0644 0.0029 0.0002 0.0035 0.0003 0.0007 0.0238 0.0009 0.0141 0.0043 0.0008 0.0094 0.1215 0.0005 0.0009 0.0001 0.0001 0.0033
Bud 0.0022 0.0004 0.0001 0.0003 0.0004 0.0002 0.0103 0.0001 0.0021 0.0041 0.0003 0.0004 0.0012 0 0.0001 0 0 0.0029
Bud Neck 0.0028 0.0002 0.0001 0.0081 0.0003 0.0062 0.0012 0.0001 0.0007 0.0016 0.0002 0.0008 0.0021 0.0001 0.0001 0 0.0001 0.0003
Bud Periphery 0.0064 0.0005 0.0001 0.0002 0.0005 0.0004 0.0037 0.0001 0.002 0.0092 0.0007 0.0004 0.001 0 0 0 0 0.0039
Bud Site 0.0131 0.0021 0.0001 0.0379 0.0005 0.0001 0.0095 0.0007 0.0114 0.0035 0.0001 0.0005 0.0045 0.0003 0.0002 0 0 0.0001
Cell Periphery 0.006 0.0004 0.0001 0.0008 0.0001 0.0001 0.001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0002
Cytoplasm 0.2141 0.4555 0.4219 0.3915 0.5969 0.5542 0.2045 0.4182 0.3184 0.6149 0.2439 0.3548 0.0018 0.5316 0.4005 0.3179 0.5418 0.4778
Cytoplasmic Foci 0.0094 0.009 0.0034 0.0073 0.0025 0.0191 0.0244 0.0043 0.0334 0.0091 0.0237 0.0103 0.2365 0.0023 0.0018 0.0017 0.0016 0.0225
Eisosomes 0.0024 0.0002 0.0001 0.0005 0 0.0001 0.0006 0.0001 0.0001 0.0002 0.0001 0.0002 0.0005 0 0.0003 0.0001 0 0
Endoplasmic Reticulum 0.0075 0.0068 0.0021 0.0034 0.002 0.0018 0.0026 0.0014 0.0061 0.0017 0.0012 0.0019 0.0003 0.0011 0.0013 0.0014 0.0066 0.0013
Endosome 0.0408 0.0463 0.0012 0.0038 0.0034 0.0062 0.0187 0.0006 0.0197 0.0017 0.0206 0.0076 0.0592 0.0002 0.0002 0.0004 0.0005 0.0007
Golgi 0.0063 0.0078 0.0001 0.0004 0.0002 0.0011 0.0068 0.0002 0.011 0.0008 0.0195 0.005 0.1652 0.0001 0.0001 0 0 0.0002
Lipid Particles 0.0155 0.0014 0.0002 0.0003 0.0001 0.0024 0.0282 0.0001 0.0056 0.0001 0.0138 0.0039 0.0481 0 0.0001 0 0.0001 0.0003
Mitochondria 0.022 0.0284 0.0004 0.0004 0.0047 0.0027 0.0093 0.0001 0.0117 0.004 0.0454 0.0012 0.0563 0 0.0001 0 0 0.0002
None 0.3566 0.424 0.5666 0.5264 0.3501 0.3855 0.6151 0.5703 0.5415 0.3404 0.6197 0.5943 0.0007 0.4625 0.5926 0.6769 0.4478 0.4829
Nuclear Periphery 0.0446 0.0017 0.0006 0.0013 0.0028 0.0009 0.002 0.0004 0.003 0.0005 0.0007 0.0005 0.0001 0.0002 0.0003 0.0003 0.0004 0.0004
Nucleolus 0.0133 0.0002 0.0001 0.0012 0.001 0.0002 0.0031 0.0001 0.0005 0.0001 0.0002 0.0001 0.0002 0 0.0001 0 0 0.0001
Nucleus 0.0847 0.0019 0.0011 0.0052 0.0304 0.0061 0.0033 0.0008 0.0048 0.0013 0.0016 0.0007 0.0001 0.0005 0.0006 0.0004 0.0003 0.0007
Peroxisomes 0.0112 0.0003 0.0001 0.0004 0.0001 0.0039 0.0226 0.0005 0.006 0.0014 0.0058 0.007 0.2983 0 0 0 0 0.0009
Punctate Nuclear 0.0148 0.0007 0.0004 0.0046 0.0007 0.0069 0.0046 0.0005 0.0048 0.0005 0.0006 0.0006 0.001 0.0002 0.0004 0.0002 0.0001 0.0009
Vacuole 0.0501 0.0083 0.0007 0.0024 0.0023 0.0009 0.0038 0.0003 0.002 0.0005 0.0008 0.0004 0.0006 0.0001 0.0002 0.0003 0.0005 0.0005
Vacuole Periphery 0.0117 0.0012 0.0001 0.0002 0.0007 0.0002 0.001 0 0.0008 0.0001 0.0004 0.0001 0.0005 0 0 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.6868 19.0515 12.3129 12.6925 18.7183 13.0549 19.8843 21.1794 16.9663 19.9562
Translational Efficiency 0.7923 0.8239 0.6109 0.6059 0.7789 1.0018 0.7774 0.4901 0.6825 0.809

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
470 1423 464 1822 2198 1491 283 1478 2668 2914 747 3300

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 714.35 762.62 910.84 865.22 756.95 758.29 885.35 1039.75 749.45 760.40 901.18 943.39
Standard Deviation 78.67 128.87 110.60 104.56 81.76 114.82 83.31 144.57 82.83 121.90 101.89 151.42
Intensity Change Log 2 — 0.094333 0.350567 0.276436 — 0.002552 0.226050 0.457967 — 0.047843 0.287849 0.372677

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000052 0.000367 0.000712 0.000543 0.000097 0.000327 0.000105 0.002013 0.000089 0.000347 0.000482 0.001201
Bud Neck 0.004896 0.013595 0.002203 0.009994 0.009289 0.019890 0.001285 0.018234 0.008515 0.016816 0.001855 0.013684
Bud Site 0.002044 0.014170 0.006290 0.055902 0.004120 0.015032 0.001875 0.040066 0.003754 0.014611 0.004617 0.048809
Cell Periphery 0.000115 0.000127 0.000179 0.000160 0.000094 0.000075 0.000215 0.000091 0.000098 0.000100 0.000192 0.000129
Cytoplasm 0.629311* 0.557797* 0.315374* 0.485725* 0.585376* 0.570998* 0.380363* 0.623304* 0.593116* 0.564552* 0.339995* 0.547344*
Cytoplasmic Foci 0.162046 0.171307 0.013412 0.011659 0.153363 0.167151 0.047861 0.001483 0.154892 0.169180 0.026463 0.007102
Eisosomes 0.000094 0.000081 0.000072 0.000036 0.000091 0.000067 0.000112 0.000014 0.000092 0.000074 0.000087 0.000026
Endoplasmic Reticulum 0.000282 0.000852 0.006057 0.001746 0.000668 0.000414 0.001695 0.000294 0.000600 0.000628 0.004404 0.001096
Endosome 0.005541 0.020631 0.004214 0.005411 0.006066 0.013276 0.004353 0.000476 0.005974 0.016868 0.004267 0.003201
Golgi 0.000389 0.003503 0.000106 0.002603 0.001383 0.002421 0.000072 0.000271 0.001208 0.002950 0.000093 0.001558
Lipid Particles 0.002689 0.004094 0.002680 0.000740 0.003826 0.002570 0.002958 0.000236 0.003625 0.003314 0.002785 0.000514
Mitochondria 0.000817 0.003343 0.000404 0.004942 0.002129 0.003470 0.000187 0.005952 0.001898 0.003408 0.000322 0.005394
Mitotic Spindle 0.000193 0.003221 0.001576 0.028480 0.003309 0.005487 0.001650 0.037258 0.002760 0.004380 0.001604 0.032411
None 0.004600 0.012027 0.003918 0.005413 0.002924 0.002667 0.005357 0.006819 0.003219 0.007238 0.004463 0.006043
Nuclear Periphery 0.000190 0.000354 0.002142 0.001220 0.000327 0.000302 0.001069 0.000811 0.000303 0.000328 0.001735 0.001036
Nuclear Periphery Foci 0.000490 0.000951 0.002826 0.000756 0.000288 0.000302 0.001241 0.000035 0.000324 0.000619 0.002226 0.000433
Nucleolus 0.001109 0.001947 0.000641 0.000276 0.000728 0.001408 0.000793 0.000294 0.000795 0.001671 0.000699 0.000284
Nucleus 0.141077* 0.100781 0.540054* 0.275817* 0.180948* 0.094404 0.411629* 0.233538* 0.173925* 0.097518 0.491400* 0.256881*
Peroxisomes 0.000950 0.006470 0.000579 0.000887 0.002320 0.007551 0.000380 0.000744 0.002078 0.007023 0.000504 0.000823
Vacuole 0.042779 0.082668 0.096341 0.104479 0.042138 0.090816 0.136631 0.027855 0.042251 0.086837 0.111605 0.070161
Vacuole Periphery 0.000337 0.001713 0.000222 0.003211 0.000517 0.001372 0.000169 0.000212 0.000485 0.001539 0.000202 0.001868

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.41 -3.97 -8.09 -0.60 1.59 -3.14 -2.36 -1.91 -1.32 -1.75 -4.32 -3.95 -2.93 -1.45 -0.64
Bud Neck -5.13 1.92 -1.98 4.48 -7.56 -6.09 10.33 -2.72 3.68 -10.78 -6.86 9.33 -1.54 5.96 -12.88
Bud Site -7.92 -1.77 -9.22 -3.41 -6.13 -6.45 2.53 -7.00 -1.59 -8.02 -9.15 -0.34 -10.84 -3.65 -8.86
Cell Periphery -0.58 -4.88 0.06 0.74 5.77 3.47 -7.72 4.86 0.62 9.20 -0.10 -10.54 0.59 0.51 10.58
Cytoplasm 5.80 24.36 22.08 19.43 -8.23 1.94 15.08 11.68 8.26 -8.66 4.46 29.44 26.30 20.20 -12.53
Cytoplasmic Foci -1.02 20.74 21.52 32.66 2.96 -1.98 21.80 41.39 34.45 11.78 -2.75 35.99 45.63 47.30 11.24
Eisosomes 1.75 0.33 7.36 6.55 8.00 4.83 -3.98 16.67 15.92 13.12 4.02 -2.33 15.05 13.22 15.11
Endoplasmic Reticulum -7.35 -18.01 -23.62 -15.60 12.21 1.92 -6.90 0.05 -4.68 7.95 -0.26 -16.90 -10.20 -16.46 13.61
Endosome -8.66 1.04 4.92 11.18 5.33 -7.71 2.01 13.59 15.76 10.51 -11.17 2.46 11.23 16.95 10.30
Golgi -6.35 4.96 -1.03 5.01 -2.06 -1.99 4.51 4.32 5.85 -1.21 -4.27 4.63 2.77 7.22 -2.16
Lipid Particles -2.76 -1.22 5.27 7.55 8.12 2.86 0.93 8.60 8.93 9.90 0.89 1.03 8.68 10.91 12.52
Mitochondria -3.34 0.72 -1.55 1.80 -2.89 -2.00 4.70 -0.81 0.94 -4.40 -2.85 4.40 -0.72 2.02 -5.05
Mitotic Spindle -3.15 -2.36 -9.02 -6.81 -7.85 -1.24 1.46 -7.02 -6.03 -7.65 -1.29 1.30 -9.71 -8.89 -10.98
None -3.46 1.72 -1.45 3.08 -7.63 0.43 -1.27 -14.47 -13.73 -5.62 -3.96 -0.45 -11.56 -0.62 -9.41
Nuclear Periphery -5.29 -20.12 -28.46 -26.00 0.75 0.32 -12.09 -19.35 -19.38 -8.14 -1.05 -21.19 -31.99 -32.07 -2.92
Nuclear Periphery Foci -4.58 -8.69 -6.06 -0.32 7.33 -0.42 -6.07 7.83 8.79 6.99 -6.10 -10.49 -6.51 0.56 9.37
Nucleolus -1.65 1.46 1.93 6.18 3.49 -2.15 0.27 1.21 3.74 1.74 -3.42 1.23 2.26 7.09 3.33
Nucleus 5.47 -26.19 -24.09 -36.00 12.12 17.58 -12.94 -24.61 -36.72 2.13 19.00 -27.55 -33.69 -51.45 10.70
Peroxisomes -8.34 2.35 3.48 9.68 0.70 -6.04 8.16 7.93 8.79 0.93 -8.76 7.70 8.69 12.82 1.04
Vacuole -9.19 -11.46 -24.28 -17.93 -4.39 -14.27 -11.55 -9.24 4.30 8.71 -17.91 -16.59 -26.83 -12.65 2.68
Vacuole Periphery -5.19 0.99 -1.75 2.19 -2.24 -3.55 5.98 7.13 5.48 2.59 -6.00 5.53 -0.27 4.30 -2.06
Standard name
Human Ortholog
Description Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (57%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Eki1

Eki1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Eki1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available