Standard name
Human Ortholog
Description Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; N-terminally propionylated in vivo; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.98 0.98 0.97 0.97 – 0.81 0.81 0.84 0.83 0.82 1.0 1.0 0.99 0.98 1.0 1.0 0.78 0.85 0.84 0.84 0.85 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 – 0.18 0.15 0.14 0.14 0.14 0 0 0 0.07 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.13 0.08 0.08 0.07 0.06 0.08
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 1 0 14 1 0 0 – 3 4 2 3 5 0 0 0 0 0 0 17 1 2 0 5 10
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 1 1 0 0 – 3 7 7 6 7 0 2 0 9 0 5 0 0 0 0 0 0
Cytoplasm 599 266 470 296 336 471 – 484 348 405 363 345 445 301 352 202 144 212 491 210 411 221 405 321
Endoplasmic Reticulum 2 0 6 3 10 12 – 106 64 66 59 58 3 1 4 15 8 11 2 1 7 2 5 6
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0
Golgi 1 0 0 0 0 0 – 0 2 2 0 1 0 0 0 1 0 1 0 0 0 0 0 0
Mitochondria 0 0 0 2 0 0 – 0 1 0 0 2 0 0 0 1 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 1 – 1 0 2 3 1 0 0 0 0 0 0 80 19 37 18 28 32
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 0 2 0 0 1
Nucleolus 0 0 0 0 0 1 – 6 3 5 6 6 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 1 0 0 1 0 1 – 2 2 2 0 0 1 0 0 0 0 1 10 4 8 7 12 15
Unique Cell Count 605 267 478 302 346 486 600 428 485 436 419 446 301 357 207 144 213 626 248 489 265 474 402
Labelled Cell Count 606 268 491 304 346 486 605 431 492 441 425 449 304 357 228 152 230 626 248 489 265 474 402


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 135.9 209.3 93.7 116.0 130.1 114.9 – 110.0 107.8 103.8 104.8 101.4 181.8 193.6 187.5 382.6 319.7 334.7 166.8 181.1 182.1
Std Deviation (1e-4) 36.1 48.9 24.9 35.2 36.5 27.3 – 31.2 32.2 33.5 33.7 31.4 45.5 46.7 45.1 134.0 138.6 134.0 57.2 49.4 58.0
Intensity Change (Log2) – – – 0.31 0.47 0.29 – 0.23 0.2 0.15 0.16 0.11 0.96 1.05 1.0 2.03 1.77 1.84 0.83 0.95 0.96


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud -2.6 -3.2 -3.8 – -3.2 -2.1 -3.1 -2.5 -1.8 -3.6 -3.0 -3.3 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 -1.2 -1.4 – -9.0 -8.6 -8.0 -8.0 -8.3 2.2 2.3 0.3 -0.7 1.6 1.3
Endoplasmic Reticulum 0 1.7 1.4 – 8.8 7.7 7.3 7.2 7.3 0 0 0 4.2 0 3.1
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 207.1412 249.4874 224.6635 196.2218 180.0823 207.5305 192.4704 226.8596 210.9196 208.7795 183.4858 231.1759 221.1965 234.384 226.4084 230.1545 210.9898 234.4413
Actin 0.0007 0.0001 0.0003 0.0204 0.0002 0.0104 0.0024 0.0001 0.0057 0.0001 0.0008 0.0002 0.0021 0.0001 0.0001 0 0.0001 0
Bud 0.0007 0.0002 0.0001 0.0004 0.0002 0.0005 0.0012 0.0001 0.0002 0 0.0025 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0
Bud Neck 0.0002 0.0001 0.0001 0.0006 0.0003 0.002 0.0002 0.0001 0.0005 0.0001 0.0004 0.0008 0.0003 0.0001 0.0001 0.0001 0.0002 0.0003
Bud Periphery 0.0005 0 0 0.0002 0.0001 0.0005 0.0006 0 0.0001 0 0.0017 0 0 0 0.0001 0 0 0
Bud Site 0.0004 0.0001 0.0001 0.0008 0.0002 0.0007 0.0004 0 0.0002 0 0.0005 0 0.0001 0 0 0 0 0
Cell Periphery 0.0007 0.0001 0.0002 0.0004 0.0012 0.0005 0.001 0.0001 0.0002 0 0.0007 0.0001 0.0006 0.0001 0.0004 0.0001 0.0006 0
Cytoplasm 0.5976 0.8707 0.7641 0.6807 0.6152 0.5201 0.6358 0.8882 0.7935 0.7004 0.7503 0.6474 0.575 0.8627 0.7226 0.6332 0.7405 0.6378
Cytoplasmic Foci 0.0091 0 0.0035 0.0044 0.0326 0.0054 0.0053 0.0001 0.0008 0 0.0204 0.0001 0.0004 0 0 0 0.0016 0
Eisosomes 0.0002 0 0.0001 0.0005 0.0003 0.0001 0.0004 0 0.0001 0 0.0003 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0007 0.0001 0.0003 0.0012 0.0002 0.0013 0.0016 0.0001 0.0003 0.0001 0.0004 0.0003 0.0008 0.0001 0.0001 0.0001 0.0003 0.0001
Endosome 0.0065 0 0.0023 0.0017 0.0011 0.0132 0.0042 0 0.0019 0 0.0003 0 0.0004 0 0 0 0.0004 0
Golgi 0.0019 0 0.0001 0.0009 0.0005 0.0091 0.0011 0 0.0033 0 0.0002 0 0.0002 0 0 0 0 0
Lipid Particles 0.0032 0 0.0009 0.0011 0.0043 0.0046 0.0035 0 0.0005 0 0.0048 0 0.0002 0 0 0 0.0002 0
Mitochondria 0.006 0.0011 0.0043 0.0035 0.0063 0.0035 0.0098 0.0001 0.0017 0.0001 0.0062 0.0002 0.0008 0.0002 0.0003 0.0002 0.0005 0.0001
None 0.007 0.0001 0.006 0.0074 0.0077 0.0048 0.0058 0.0002 0.0012 0.0001 0.0077 0.0001 0.0009 0.0001 0.0002 0.0001 0.0041 0.0001
Nuclear Periphery 0.0213 0.0085 0.016 0.0156 0.0223 0.0227 0.0245 0.0085 0.0141 0.0194 0.0148 0.0206 0.0248 0.0111 0.0162 0.0195 0.0163 0.0159
Nucleolus 0.017 0.0002 0.0082 0.0294 0.074 0.0112 0.0223 0.0002 0.0043 0.0001 0.0186 0.0006 0.0024 0.0001 0.0024 0.0006 0.0065 0.0001
Nucleus 0.313 0.118 0.1883 0.2241 0.205 0.3724 0.2707 0.1015 0.1687 0.2786 0.1625 0.3285 0.3885 0.1245 0.2555 0.3453 0.2131 0.345
Peroxisomes 0.0026 0 0.0002 0.0004 0.0147 0.0005 0.0018 0 0.0002 0 0.0008 0 0 0 0 0 0 0
Punctate Nuclear 0.003 0.0001 0.0007 0.0031 0.0018 0.0015 0.0011 0.0002 0.0008 0.0005 0.0023 0.0004 0.001 0.0002 0.0002 0.0002 0.0003 0.0003
Vacuole 0.0052 0.0003 0.0022 0.0016 0.0077 0.0089 0.0037 0.0003 0.0007 0.0002 0.0025 0.0004 0.0009 0.0004 0.0011 0.0003 0.0114 0.0002
Vacuole Periphery 0.0026 0.0001 0.0019 0.0015 0.0041 0.0062 0.0027 0.0001 0.0009 0.0001 0.0014 0.0002 0.0003 0.0001 0.0004 0.0002 0.0037 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2398.6072 2305.5787 2367.9327 2276.2661 2494.063 2566.4195 2348.5041 2238.2486 2744.8264 2371.212
Translational Efficiency 1.6761 1.6416 1.6648 1.7906 1.8591 1.7515 1.8655 2.6025 2.0394 2.2263

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; N-terminally propionylated in vivo; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Cpr1

Cpr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cpr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available