Standard name
Human Ortholog
Description Aspartic beta semi-aldehyde dehydrogenase; catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0.06 0.05 0 0.08 0.07 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.98 0.99 0.99 0.98 0.97 0.9 0.87 0.87 0.85 0.91 0.82 1.0 1.0 1.0 0.99 0.99 0.97 0.95 0.97 0.97 0.98 0.96 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.07 0.09 0.08 0.08 0 0.08 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 2 4 4 4 8 0 0 0 0 0 0 0 0 0 0 0 0
Bud 2 1 1 0 0 0 1 5 1 1 3 4 0 0 1 0 0 0 0 0 0 0 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 5 1 4 10 16 18 10 27 14 30 3 4 8 18 6 5 0 0 0 0 0 0
Cytoplasm 222 165 147 165 257 196 242 299 211 296 192 240 243 337 332 556 371 458 199 157 133 165 296 351
Endoplasmic Reticulum 1 0 1 2 4 3 19 32 19 27 7 23 0 0 2 23 22 14 0 1 0 0 1 6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 1 2 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 1 2 1 1 0 0 0 0 0 1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 4 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 2 3 7 4 10 0 0 0 0 3 4 0 0 0 0 2 2
Unique Cell Count 223 168 149 167 262 202 268 343 242 348 211 294 243 337 333 563 374 470 210 163 138 169 309 371
Labelled Cell Count 226 169 154 168 265 209 281 358 249 367 226 316 246 341 343 598 403 490 210 163 138 169 309 371


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 93.4 139.4 99.0 96.9 87.2 70.5 55.5 54.2 47.7 43.5 40.1 41.8 124.4 109.3 107.8 161.0 185.0 181.7 108.2 104.6 103.5
Std Deviation (1e-4) 18.0 25.4 23.8 22.0 19.3 17.1 14.5 13.9 13.9 11.7 11.8 12.7 27.1 25.6 26.9 35.1 45.0 54.0 26.4 28.5 24.8
Intensity Change (Log2) – – – -0.03 -0.18 -0.49 -0.84 -0.87 -1.05 -1.19 -1.3 -1.24 0.33 0.14 0.12 0.7 0.9 0.88 0.13 0.08 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.7 1.2 0.9 0.4 1.8 1.4 2.5 0 0 0 0 0 0
Cytoplasm 0.1 -0.4 -1.0 -3.3 -4.0 -4.0 -4.5 -3.1 -5.1 1.8 2.1 1.3 0.1 0.6 -0.9
Endoplasmic Reticulum 0 0 0 2.9 3.5 3.1 3.1 0 3.1 0 0 0 2.1 2.6 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0007 0.0001 0.0068 0.0001 0.0334 0.0008 0.0025 0 0.0013 0.0002 0.0002 0.0001 0.0535 0.0001 0.0001 0 0.0065 0.0002
Bud 0.0005 0.0001 0.0003 0.0001 0.001 0.0005 0.0008 0 0.0006 0.0004 0.0005 0 0.0014 0 0 0 0.0054 0
Bud Neck 0.0003 0.0002 0.0004 0.0001 0.0008 0.0006 0.0002 0.0001 0.0002 0.0002 0.0004 0.0004 0.0001 0.0001 0.0001 0.0001 0.0002 0.0003
Bud Periphery 0.0003 0 0.0003 0 0.0008 0.0001 0.0004 0 0.0005 0.0002 0.0003 0 0.0012 0 0 0 0.0029 0
Bud Site 0.0008 0.0001 0.0004 0 0.0012 0 0.0028 0 0.0004 0.0002 0.0001 0 0.0007 0 0 0 0.0019 0
Cell Periphery 0.0007 0.0006 0.0008 0.0002 0.0013 0.0041 0.0007 0.0002 0.0004 0.0006 0.0004 0 0.0005 0 0 0 0.0018 0
Cytoplasm 0.9252 0.9914 0.9355 0.9755 0.8114 0.9728 0.953 0.9971 0.9798 0.8874 0.9466 0.9957 0.4456 0.998 0.9916 0.9971 0.0114 0.9959
Cytoplasmic Foci 0.0122 0.0001 0.0063 0.0011 0.011 0.0011 0.0094 0.0002 0.0017 0.0288 0.009 0.0003 0.0014 0.0001 0.0001 0.0001 0.0041 0.0003
Eisosomes 0.0002 0.0001 0.0006 0 0.0004 0 0.0003 0 0.0001 0.0006 0 0 0.0011 0 0 0 0.0005 0
Endoplasmic Reticulum 0.0008 0.0002 0.0037 0.0002 0.0011 0.0006 0.0004 0.0001 0.0004 0.0002 0.0003 0.0001 0.003 0 0 0 0.0002 0.0001
Endosome 0.0018 0 0.0045 0.0001 0.0051 0.0005 0.0018 0 0.0011 0.0004 0.0004 0.0001 0.0027 0 0.0001 0 0.0054 0
Golgi 0.0006 0 0.0018 0 0.007 0.0001 0.0005 0 0.0012 0.0001 0 0 0.0009 0 0 0 0.0021 0
Lipid Particles 0.0048 0.0001 0.0041 0.0001 0.008 0.0007 0.0008 0.0002 0.0002 0.0052 0.0005 0 0.0004 0 0 0 0.0016 0
Mitochondria 0.0062 0.0005 0.0069 0.0005 0.0132 0.0002 0.0022 0 0.0067 0.0108 0.0029 0 0.0508 0 0 0 0.8309 0.0001
None 0.0071 0.0003 0.0049 0.0098 0.0114 0.0005 0.0066 0.0003 0.0008 0.023 0.02 0.0001 0.0103 0.0001 0.0001 0 0.0037 0.0002
Nuclear Periphery 0.0069 0.0011 0.0024 0.0027 0.006 0.0025 0.002 0.0003 0.0008 0.0008 0.0025 0.0005 0.2554 0.0004 0.0017 0.0003 0.0022 0.0005
Nucleolus 0.0112 0.0021 0.0082 0.001 0.0379 0.0002 0.0033 0.0001 0.0012 0.0238 0.002 0 0.0023 0 0 0 0.0086 0
Nucleus 0.0107 0.0023 0.0036 0.005 0.0311 0.0038 0.0075 0.0008 0.0017 0.0033 0.0082 0.002 0.1085 0.001 0.0034 0.0022 0.0113 0.0022
Peroxisomes 0.0031 0 0.0021 0 0.003 0 0.0004 0 0.0001 0.0013 0.0002 0 0.0008 0 0 0 0.0064 0
Punctate Nuclear 0.0012 0 0.0014 0.0003 0.0037 0.0002 0.0015 0 0.0001 0.0011 0.0004 0 0.0316 0 0 0 0.0001 0.0001
Vacuole 0.003 0.0006 0.0024 0.0019 0.0052 0.0091 0.002 0.0004 0.0006 0.0062 0.0037 0.0004 0.0022 0 0.0014 0 0.0209 0
Vacuole Periphery 0.0018 0.0002 0.0026 0.0012 0.0058 0.0014 0.0008 0 0.0003 0.0053 0.0014 0.0001 0.0256 0 0.0013 0 0.0717 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 162.2949 212.9063 261.9763 220.6526 182.4096 250.0871 323.3582 274.0323 297.2855 251.1053
Translational Efficiency 3.0845 2.7048 2.181 2.4444 3.0136 2.5245 2.3955 2.5837 2.3481 2.3665

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
35 346 2061 1550 1681 1522 438 1418 1716 1868 2499 2968

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2865.27 12129.38 5936.32 3682.29 6577.23 10008.11 4575.38 3577.76 6501.52 10401.02 5697.79 3632.35
Standard Deviation 1785.32 2511.47 1171.32 890.81 1472.30 2320.35 932.96 887.12 1569.64 2496.83 1245.72 890.58
Intensity Change Log 2 — 2.081763 1.050898 0.361932 — 0.605618 -0.523589 -0.878424 — 1.229251 0.154755 -0.379189

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002157 0.000094 0.000047 0.000243 0.000048 0.000058 0.000170 0.001453 0.000091 0.000064 0.000068 0.000821
Bud Neck 0.008302 0.000941 0.000440 0.003841 0.000265 0.000513 0.004275 0.003106 0.000428 0.000592 0.001113 0.003490
Bud Site 0.027744 0.002810 0.001111 0.007746 0.000916 0.001253 0.003578 0.017783 0.001463 0.001542 0.001543 0.012541
Cell Periphery 0.044697 0.012319 0.001053 0.000629 0.003312 0.002599 0.001632 0.000372 0.004156 0.004399 0.001154 0.000506
Cytoplasm 0.229982* 0.825602* 0.893364* 0.781441* 0.909164* 0.874924* 0.709650* 0.783459* 0.895311* 0.865788* 0.861164* 0.782405*
Cytoplasmic Foci 0.034228 0.017890 0.003916 0.011798 0.004172 0.008348 0.013785 0.029601 0.004785 0.010115 0.005645 0.020304
Eisosomes 0.000044 0.000002 0.000001 0.000004 0.000001 0.000001 0.000003 0.000009 0.000002 0.000001 0.000001 0.000006
Endoplasmic Reticulum 0.004378 0.000032 0.000019 0.000105 0.000065 0.000076 0.000104 0.000169 0.000153 0.000067 0.000034 0.000136
Endosome 0.023383 0.000123 0.000044 0.002836 0.000078 0.000074 0.000744 0.002221 0.000554 0.000083 0.000167 0.002542
Golgi 0.075736 0.005947 0.000854 0.005638 0.005664 0.001939 0.001956 0.003517 0.007093 0.002682 0.001047 0.004625
Lipid Particles 0.046418 0.009354 0.001292 0.002162 0.002274 0.001946 0.004300 0.002753 0.003175 0.003318 0.001819 0.002444
Mitochondria 0.004687 0.000085 0.000081 0.000556 0.000123 0.000114 0.000175 0.001758 0.000216 0.000109 0.000098 0.001130
Mitotic Spindle 0.002313 0.001414 0.000215 0.023778 0.000315 0.000413 0.001111 0.043610 0.000356 0.000599 0.000372 0.033253
None 0.054082 0.009990 0.005633 0.003800 0.007578 0.008622 0.005780 0.006250 0.008526 0.008876 0.005658 0.004970
Nuclear Periphery 0.001895 0.000451 0.000127 0.000564 0.000214 0.000282 0.000531 0.000224 0.000248 0.000313 0.000198 0.000402
Nuclear Periphery Foci 0.055439 0.004015 0.001178 0.004319 0.002902 0.001778 0.004776 0.004435 0.003973 0.002193 0.001809 0.004375
Nucleolus 0.013845 0.000061 0.000052 0.000611 0.000210 0.000066 0.000206 0.000276 0.000488 0.000065 0.000079 0.000451
Nucleus 0.238819* 0.078541 0.079119 0.110798 0.047078 0.087177 0.203061* 0.073179 0.050988 0.085578 0.100843 0.092825
Peroxisomes 0.001057 0.000019 0.000009 0.000163 0.000008 0.000015 0.000015 0.000729 0.000029 0.000015 0.000010 0.000433
Vacuole 0.103564 0.028590 0.011113 0.035843 0.010971 0.009027 0.043496 0.023756 0.012860 0.012650 0.016789 0.030068
Vacuole Periphery 0.027227 0.001720 0.000333 0.003125 0.004643 0.000775 0.000652 0.001339 0.005104 0.000950 0.000389 0.002271

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.38 2.46 2.25 -3.77 -6.95 -1.03 -9.05 -2.14 -2.08 -1.28 0.69 -0.37 -2.57 -2.73 -2.54
Bud Neck 2.26 2.48 1.16 -9.11 -12.66 -3.33 -11.86 -14.46 -12.62 4.61 -2.93 -9.66 -18.25 -16.61 -10.68
Bud Site 2.01 2.16 1.59 -6.01 -9.43 -2.48 -4.88 -10.38 -10.10 -7.10 -0.70 -1.19 -12.42 -12.81 -12.50
Cell Periphery 2.24 3.09 3.14 7.74 7.08 0.71 2.68 6.42 8.03 9.52 -0.93 5.01 6.73 10.68 10.87
Cytoplasm -10.29 -11.88 -8.55 7.90 24.86 8.73 27.44 26.92 20.48 -5.96 7.84 12.72 31.84 25.23 21.62
Cytoplasmic Foci 1.16 2.96 1.52 1.13 -7.91 -6.46 -9.58 -14.28 -12.53 -6.76 -8.21 -6.64 -15.93 -11.71 -13.44
Eisosomes 4.22 4.42 4.17 -0.90 -6.83 -1.41 -8.93 -3.08 -2.82 0.43 0.53 -0.16 -3.30 -3.87 -3.53
Endoplasmic Reticulum 3.55 3.56 3.52 -3.95 -5.68 -0.10 -0.95 -0.22 -0.04 0.61 1.60 3.31 2.08 -0.18 -2.83
Endosome 1.67 1.68 1.57 -4.66 -4.94 -0.59 -2.82 -5.85 -5.75 -2.70 1.54 1.34 -2.83 -7.18 -6.82
Golgi 2.34 2.51 2.46 3.26 -1.81 5.54 5.87 5.39 0.77 0.17 4.81 6.77 5.14 1.45 -2.12
Lipid Particles 2.08 2.67 2.47 3.36 -7.33 -0.44 -4.72 -7.47 -6.93 -0.44 -1.48 0.47 -5.17 -3.66 -8.01
Mitochondria 1.84 1.85 1.77 -3.06 -3.21 0.22 -2.50 -1.74 -1.77 -1.49 1.77 1.92 -1.47 -2.34 -2.40
Mitotic Spindle 0.71 2.40 -7.79 -8.18 -8.77 -1.86 -3.38 -12.75 -12.69 -12.53 -2.55 -1.30 -15.36 -15.20 -15.31
None 1.28 1.40 1.45 2.93 6.60 -1.09 4.32 3.47 4.20 -0.74 -0.11 3.70 4.70 6.42 2.98
Nuclear Periphery 2.32 2.97 2.19 -0.91 -7.65 -0.99 -7.98 -7.51 -1.69 4.21 -1.25 -1.53 -7.34 -2.15 -7.31
Nuclear Periphery Foci 2.15 2.28 2.13 -0.59 -5.49 1.71 -2.62 -4.18 -5.23 -0.71 2.18 2.73 -1.99 -5.37 -6.67
Nucleolus 2.95 2.95 2.85 -4.67 -4.80 1.06 -0.45 -1.01 -8.04 -2.06 2.40 2.27 0.33 -7.08 -6.84
Nucleus 2.57 2.52 1.80 -6.47 -9.60 -12.46 -22.52 -11.14 1.83 17.38 -11.44 -18.26 -17.85 -4.77 1.93
Peroxisomes 1.71 1.73 1.51 -5.69 -6.40 -2.01 -6.44 -6.14 -6.05 -5.91 0.93 1.23 -6.75 -7.23 -7.32
Vacuole 2.85 3.81 0.67 -8.44 -18.23 -0.83 -12.16 -17.27 -16.51 3.64 -2.39 -9.06 -24.18 -21.96 -16.08
Vacuole Periphery 3.82 4.02 3.87 0.84 -2.81 4.78 5.18 5.05 1.04 -0.33 5.17 6.00 5.10 0.03 -3.04
Standard name
Human Ortholog
Description Aspartic beta semi-aldehyde dehydrogenase; catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Hom2

Hom2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hom2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available