Standard name | |
---|---|
Human Ortholog | |
Description | mRNA export factor; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 5 | 2 | 1 | 6 | 6 |
Bud | 0 | 2 | 2 | 2 | 7 | 7 | 5 | 6 | 14 | – | 15 | 16 | 1 | 1 | 5 | 1 | 5 | 1 | 0 | 2 | 2 | 3 | 4 | 8 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 2 | 4 |
Bud Site | 0 | 0 | 0 | 1 | 3 | 2 | 10 | 8 | 12 | – | 7 | 12 | 0 | 1 | 3 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 5 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 25 | 19 | 18 | 26 | 29 | 32 | 29 | 31 | – | 18 | 21 | 42 | 84 | 94 | 252 | 157 | 127 | 1 | 9 | 0 | 3 | 5 | 4 |
Endoplasmic Reticulum | 4 | 3 | 7 | 4 | 1 | 0 | 2 | 1 | 2 | – | 2 | 4 | 0 | 6 | 3 | 83 | 70 | 53 | 0 | 0 | 4 | 2 | 4 | 2 |
Endosome | 106 | 53 | 25 | 3 | 39 | 11 | 7 | 8 | 3 | – | 3 | 1 | 72 | 99 | 32 | 343 | 281 | 293 | 76 | 85 | 81 | 56 | 110 | 123 |
Golgi | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 1 | 5 | 6 | 2 | 3 | 5 | 5 |
Mitochondria | 20 | 97 | 207 | 240 | 223 | 293 | 397 | 403 | 520 | – | 377 | 401 | 152 | 96 | 219 | 121 | 132 | 110 | 19 | 35 | 35 | 39 | 48 | 52 |
Nucleus | 8 | 15 | 8 | 13 | 11 | 29 | 25 | 28 | 25 | – | 26 | 16 | 8 | 14 | 12 | 67 | 72 | 62 | 1 | 10 | 2 | 11 | 4 | 3 |
Nuclear Periphery | 181 | 115 | 85 | 80 | 83 | 54 | 117 | 48 | 107 | – | 72 | 83 | 122 | 117 | 105 | 292 | 219 | 277 | 274 | 198 | 204 | 174 | 237 | 275 |
Nucleolus | 28 | 11 | 9 | 8 | 11 | 12 | 29 | 24 | 30 | – | 30 | 25 | 19 | 9 | 13 | 41 | 37 | 41 | 2 | 15 | 7 | 13 | 11 | 7 |
Peroxisomes | 3 | 5 | 0 | 0 | 9 | 2 | 1 | 0 | 3 | – | 0 | 2 | 1 | 4 | 9 | 10 | 10 | 9 | 5 | 5 | 11 | 5 | 16 | 25 |
SpindlePole | 7 | 6 | 11 | 5 | 7 | 9 | 18 | 15 | 17 | – | 8 | 12 | 5 | 14 | 12 | 103 | 108 | 96 | 14 | 18 | 19 | 24 | 35 | 26 |
Vac/Vac Membrane | 136 | 139 | 38 | 41 | 50 | 28 | 31 | 14 | 21 | – | 17 | 16 | 70 | 70 | 103 | 432 | 290 | 222 | 3 | 14 | 15 | 20 | 15 | 13 |
Unique Cell Count | 319 | 338 | 309 | 318 | 358 | 351 | 498 | 463 | 582 | 422 | 447 | 364 | 404 | 482 | 1265 | 1006 | 924 | 417 | 416 | 403 | 368 | 518 | 572 | |
Labelled Cell Count | 503 | 473 | 412 | 415 | 470 | 476 | 675 | 584 | 786 | 575 | 609 | 493 | 515 | 611 | 1752 | 1387 | 1297 | 417 | 416 | 403 | 368 | 518 | 572 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.8 | 5.5 | 4.3 | 4.2 | 4.5 | 3.9 | 3.9 | 3.8 | 3.4 | – | 3.5 | 3.3 | 5.0 | 5.1 | 4.5 | 6.8 | 6.8 | 6.7 | 5.4 | 5.2 | 5.7 |
Std Deviation (1e-4) | 1.0 | 1.2 | 0.9 | 1.3 | 1.5 | 1.4 | 1.4 | 1.4 | 1.0 | – | 1.3 | 1.1 | 1.1 | 1.3 | 1.2 | 1.7 | 1.7 | 1.7 | 1.5 | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.07 | -0.14 | -0.16 | -0.18 | -0.36 | – | -0.31 | -0.38 | 0.21 | 0.24 | 0.06 | 0.65 | 0.65 | 0.64 | 0.32 | 0.27 | 0.41 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.3 | 0.6 | 1.0 | 0.2 | 0.1 | -0.5 | – | -1.1 | -0.9 | 2.4 | 5.5 | 5.2 | 5.7 | 4.3 | 3.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -0.8 | 0 | 2.9 | 3.1 | 2.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 4.3 | 5.7 | 0 | 7.1 | 7.2 | 8.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | -6.5 | -11.6 | 0 | -22.3 | -18.9 | -19.2 |
Nucleus | 1.0 | 0.4 | 3.2 | 1.7 | 2.2 | 1.3 | – | 2.3 | 0.8 | -0.3 | 0.7 | -0.1 | 2.0 | 2.9 | 2.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1.7 | 0.4 | 0 | -1.6 | -2.1 | 0.8 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1.5 | -0.6 | 0 | 0.3 | 0.6 | 1.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | -1.9 | -0.1 | 0 | 2.8 | 3.8 | 3.7 |
Vacuole | 0.2 | 0.6 | -1.8 | -3.0 | -5.0 | -5.0 | – | -4.2 | -4.6 | 2.4 | 1.9 | 3.3 | 7.5 | 5.9 | 4.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.4405 | 5.1132 | 4.6584 | 4.3586 | 4.3243 | 4.8546 | 3.4472 | 3.4185 | 3.3287 | 3.1909 | 3.5757 | 3.24 | 3.9742 | 3.8744 | 3.6676 | 3.7235 | 3.7999 | 4.0462 |
Actin | 0.0205 | 0.0014 | 0.0074 | 0.0229 | 0.0019 | 0.0027 | 0.0147 | 0.0027 | 0.0172 | 0.0252 | 0.0029 | 0.0014 | 0.03 | 0.0049 | 0.025 | 0.0079 | 0.001 | 0.0077 |
Bud | 0.0004 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0006 | 0.0008 | 0.0003 | 0.0002 | 0 | 0 |
Bud Neck | 0.0041 | 0.002 | 0.0016 | 0.0126 | 0.0018 | 0.0026 | 0.0018 | 0.0004 | 0.0004 | 0.0245 | 0.0008 | 0.0007 | 0.0022 | 0.0009 | 0.0002 | 0.0016 | 0.0002 | 0.0004 |
Bud Periphery | 0.0014 | 0.0004 | 0.0012 | 0.002 | 0.0005 | 0.0004 | 0.001 | 0.0008 | 0.0006 | 0.0016 | 0.001 | 0.0003 | 0.0014 | 0.0015 | 0.0009 | 0.0006 | 0.0001 | 0.0002 |
Bud Site | 0.0012 | 0.0049 | 0.0017 | 0.0018 | 0.0006 | 0.0004 | 0.0016 | 0.0018 | 0.0023 | 0.0012 | 0.0006 | 0.0002 | 0.0026 | 0.0059 | 0.001 | 0.0005 | 0.0001 | 0.0001 |
Cell Periphery | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0 |
Cytoplasm | 0.0095 | 0.0088 | 0.0017 | 0.004 | 0.0024 | 0.0028 | 0.0065 | 0.0021 | 0.0038 | 0.002 | 0.0093 | 0.0024 | 0.0027 | 0.0075 | 0.0018 | 0.0056 | 0.0028 | 0.0025 |
Cytoplasmic Foci | 0.0033 | 0.0028 | 0.0028 | 0.0055 | 0.0042 | 0.0019 | 0.0115 | 0.0018 | 0.0142 | 0.0064 | 0.0094 | 0.0024 | 0.0088 | 0.0047 | 0.0094 | 0.0105 | 0.0022 | 0.0052 |
Eisosomes | 0.0004 | 0.0001 | 0.0003 | 0.0004 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0007 | 0.0003 | 0.0001 | 0.0004 | 0.0004 | 0.0009 | 0.0003 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0099 | 0.0155 | 0.0077 | 0.007 | 0.0089 | 0.0058 | 0.0168 | 0.0091 | 0.007 | 0.009 | 0.0067 | 0.0056 | 0.0084 | 0.0231 | 0.0054 | 0.0116 | 0.0037 | 0.0107 |
Endosome | 0.0191 | 0.0154 | 0.0131 | 0.0112 | 0.0113 | 0.0111 | 0.0312 | 0.0077 | 0.0148 | 0.0317 | 0.0508 | 0.0178 | 0.035 | 0.0177 | 0.0084 | 0.029 | 0.0131 | 0.0099 |
Golgi | 0.0059 | 0.0021 | 0.0037 | 0.0051 | 0.0029 | 0.0022 | 0.0129 | 0.0019 | 0.0098 | 0.0224 | 0.0081 | 0.0025 | 0.0137 | 0.0022 | 0.0069 | 0.0045 | 0.0029 | 0.0028 |
Lipid Particles | 0.0419 | 0.0266 | 0.0326 | 0.0377 | 0.068 | 0.0247 | 0.092 | 0.026 | 0.0568 | 0.0874 | 0.0612 | 0.014 | 0.0643 | 0.0624 | 0.052 | 0.0643 | 0.073 | 0.0457 |
Mitochondria | 0.006 | 0.0015 | 0.0081 | 0.0061 | 0.0049 | 0.0019 | 0.0073 | 0.0107 | 0.0048 | 0.0434 | 0.027 | 0.0078 | 0.0151 | 0.0043 | 0.0164 | 0.0032 | 0.002 | 0.0013 |
None | 0.0066 | 0.0055 | 0.0023 | 0.0043 | 0.003 | 0.0052 | 0.0044 | 0.0021 | 0.0068 | 0.0045 | 0.0519 | 0.0007 | 0.0034 | 0.0115 | 0.0135 | 0.03 | 0.0014 | 0.0072 |
Nuclear Periphery | 0.7002 | 0.68 | 0.7282 | 0.6446 | 0.6674 | 0.7602 | 0.6777 | 0.7836 | 0.7121 | 0.6144 | 0.6226 | 0.8337 | 0.7331 | 0.6813 | 0.7465 | 0.7258 | 0.8059 | 0.8029 |
Nucleolus | 0.01 | 0.0207 | 0.0204 | 0.0226 | 0.0119 | 0.0253 | 0.0061 | 0.0093 | 0.0106 | 0.0035 | 0.0205 | 0.0125 | 0.002 | 0.0218 | 0.0047 | 0.0075 | 0.0032 | 0.0064 |
Nucleus | 0.051 | 0.1214 | 0.0523 | 0.0813 | 0.1382 | 0.0773 | 0.0365 | 0.0484 | 0.0379 | 0.0328 | 0.0618 | 0.052 | 0.0082 | 0.0658 | 0.0354 | 0.0157 | 0.0418 | 0.0244 |
Peroxisomes | 0.0021 | 0.0009 | 0.0022 | 0.0037 | 0.0021 | 0.0011 | 0.0094 | 0.0022 | 0.0048 | 0.0059 | 0.0045 | 0.001 | 0.0327 | 0.0013 | 0.0117 | 0.0058 | 0.0008 | 0.0077 |
Punctate Nuclear | 0.0895 | 0.0706 | 0.0943 | 0.1096 | 0.0535 | 0.0625 | 0.0505 | 0.0688 | 0.0756 | 0.0625 | 0.035 | 0.0328 | 0.0199 | 0.0584 | 0.0376 | 0.06 | 0.0246 | 0.0531 |
Vacuole | 0.0056 | 0.0053 | 0.003 | 0.0026 | 0.0046 | 0.003 | 0.0034 | 0.0022 | 0.002 | 0.0039 | 0.0043 | 0.0025 | 0.0031 | 0.0066 | 0.0019 | 0.0035 | 0.0022 | 0.0017 |
Vacuole Periphery | 0.0111 | 0.0137 | 0.0147 | 0.0143 | 0.0108 | 0.0083 | 0.0137 | 0.0176 | 0.0178 | 0.016 | 0.0207 | 0.0094 | 0.0119 | 0.0164 | 0.02 | 0.0116 | 0.0191 | 0.0099 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.0058 | 11.6163 | 10.4937 | 19.6181 | 11.9035 | 12.705 | 14.4262 | 16.3715 | 14.9326 | 18.2475 |
Translational Efficiency | 0.4539 | 0.4151 | 0.6056 | 0.3297 | 0.4857 | 0.4794 | 0.422 | 0.3205 | 0.4431 | 0.3432 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
410 | 1692 | 216 | 1522 | 1814 | 1960 | 201 | 1514 | 2224 | 3652 | 417 | 3036 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 683.32 | 799.98 | 1112.40 | 915.19 | 723.49 | 761.52 | 1013.52 | 855.27 | 716.08 | 779.34 | 1064.74 | 885.31 |
Standard Deviation | 70.08 | 110.59 | 133.70 | 117.32 | 94.42 | 98.44 | 105.45 | 137.64 | 91.76 | 106.00 | 130.61 | 131.32 |
Intensity Change Log 2 | — | 0.227403 | 0.703042 | 0.421510 | — | 0.073909 | 0.486330 | 0.241407 | — | 0.150505 | 0.595660 | 0.331697 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000218 | 0.000120 | 0.000952 | 0.000150 | 0.000090 | 0.000187 | 0.000746 | 0.000596 | 0.000113 | 0.000156 | 0.000852 | 0.000372 |
Bud Neck | 0.000196 | 0.000413 | 0.002541 | 0.001892 | 0.000322 | 0.000479 | 0.001269 | 0.001798 | 0.000299 | 0.000448 | 0.001928 | 0.001845 |
Bud Site | 0.000456 | 0.000583 | 0.000614 | 0.001640 | 0.000111 | 0.000389 | 0.000232 | 0.002728 | 0.000175 | 0.000479 | 0.000430 | 0.002183 |
Cell Periphery | 0.000114 | 0.000038 | 0.000038 | 0.000024 | 0.000035 | 0.000036 | 0.000034 | 0.000039 | 0.000049 | 0.000037 | 0.000036 | 0.000032 |
Cytoplasm | 0.011905 | 0.011275 | 0.000573 | 0.007351 | 0.011610 | 0.008444 | 0.001368 | 0.014656 | 0.011664 | 0.009756 | 0.000956 | 0.010994 |
Cytoplasmic Foci | 0.012479 | 0.007076 | 0.000340 | 0.001389 | 0.005611 | 0.006736 | 0.000388 | 0.003433 | 0.006877 | 0.006894 | 0.000363 | 0.002408 |
Eisosomes | 0.000027 | 0.000013 | 0.000015 | 0.000005 | 0.000016 | 0.000016 | 0.000020 | 0.000010 | 0.000018 | 0.000015 | 0.000017 | 0.000008 |
Endoplasmic Reticulum | 0.005293 | 0.005438 | 0.010558 | 0.005294 | 0.004520 | 0.004747 | 0.012248 | 0.004857 | 0.004662 | 0.005067 | 0.011373 | 0.005076 |
Endosome | 0.003416 | 0.006530 | 0.001087 | 0.003971 | 0.003747 | 0.007149 | 0.001567 | 0.006052 | 0.003686 | 0.006862 | 0.001318 | 0.005009 |
Golgi | 0.001330 | 0.002237 | 0.000163 | 0.001792 | 0.001066 | 0.003207 | 0.000112 | 0.002629 | 0.001115 | 0.002757 | 0.000139 | 0.002209 |
Lipid Particles | 0.324688* | 0.087327 | 0.001715 | 0.002625 | 0.082334 | 0.089091 | 0.002819 | 0.004035 | 0.127012* | 0.088273 | 0.002247 | 0.003328 |
Mitochondria | 0.009189 | 0.007359 | 0.001310 | 0.002785 | 0.007078 | 0.010412 | 0.001529 | 0.005909 | 0.007467 | 0.008997 | 0.001415 | 0.004343 |
Mitotic Spindle | 0.006936 | 0.041415 | 0.131670* | 0.330379* | 0.022207 | 0.056066 | 0.060750 | 0.256020* | 0.019392 | 0.049278 | 0.097485 | 0.293298* |
None | 0.006115 | 0.003510 | 0.000264 | 0.000564 | 0.003763 | 0.003510 | 0.000470 | 0.001666 | 0.004196 | 0.003510 | 0.000363 | 0.001114 |
Nuclear Periphery | 0.055183 | 0.071272 | 0.219778* | 0.167447* | 0.105898* | 0.094584 | 0.183236* | 0.151320* | 0.096548 | 0.083783 | 0.202164* | 0.159405* |
Nuclear Periphery Foci | 0.529816* | 0.720702* | 0.530442* | 0.437072* | 0.695258* | 0.660264* | 0.628084* | 0.487130* | 0.664758* | 0.688265* | 0.577507* | 0.462035* |
Nucleolus | 0.016939 | 0.012187 | 0.038904 | 0.005978 | 0.025896 | 0.025293 | 0.037770 | 0.012721 | 0.024245 | 0.019221 | 0.038358 | 0.009341 |
Nucleus | 0.011862 | 0.017504 | 0.055442 | 0.018052 | 0.027610 | 0.023793 | 0.064004 | 0.032427 | 0.024707 | 0.020880 | 0.059569 | 0.025221 |
Peroxisomes | 0.003084 | 0.000892 | 0.000706 | 0.000124 | 0.000589 | 0.001106 | 0.000614 | 0.000435 | 0.001049 | 0.001007 | 0.000662 | 0.000279 |
Vacuole | 0.000306 | 0.002529 | 0.001980 | 0.008913 | 0.001415 | 0.002472 | 0.002419 | 0.008062 | 0.001211 | 0.002498 | 0.002192 | 0.008488 |
Vacuole Periphery | 0.000447 | 0.001580 | 0.000908 | 0.002553 | 0.000824 | 0.002019 | 0.000322 | 0.003477 | 0.000755 | 0.001815 | 0.000626 | 0.003014 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.83 | -4.71 | -0.20 | -3.89 | 5.69 | -1.36 | -6.27 | -2.68 | -2.09 | 0.81 | -0.93 | -8.67 | -3.09 | -2.59 | 3.87 |
Bud Neck | -1.88 | -4.58 | -8.86 | -8.08 | 0.64 | -1.79 | -4.39 | -8.04 | -7.40 | -2.79 | -2.01 | -5.61 | -11.76 | -10.95 | -0.55 |
Bud Site | -0.15 | -1.01 | -2.86 | -2.56 | -1.23 | -2.19 | -2.63 | -3.48 | -3.21 | -3.32 | -1.33 | -1.48 | -4.76 | -4.13 | -3.03 |
Cell Periphery | 2.03 | 1.67 | 2.28 | 1.70 | 1.91 | -0.32 | -0.35 | -1.51 | -1.19 | -0.89 | 1.62 | 0.64 | 1.79 | 0.37 | 1.24 |
Cytoplasm | 0.11 | 3.71 | -0.40 | -0.80 | -8.35 | 1.66 | 7.61 | -4.20 | -5.59 | -9.77 | 1.12 | 8.87 | -3.32 | -4.74 | -12.80 |
Cytoplasmic Foci | 2.13 | 4.55 | 4.15 | 6.10 | -3.52 | -1.29 | 7.71 | 2.76 | 4.31 | -5.51 | 0.20 | 8.80 | 5.78 | 7.52 | -6.53 |
Eisosomes | 3.26 | 2.12 | 4.37 | 4.62 | 3.84 | -0.72 | -1.37 | 2.00 | 2.64 | 2.09 | 2.44 | -0.34 | 5.96 | 5.04 | 3.84 |
Endoplasmic Reticulum | -0.25 | -4.55 | -1.03 | -0.85 | 3.62 | -0.34 | -6.10 | -2.26 | -1.68 | 5.16 | -0.86 | -7.95 | -2.53 | -1.65 | 6.17 |
Endosome | -3.30 | 3.97 | -0.95 | 2.60 | -5.29 | -3.77 | 3.12 | -3.12 | 0.92 | -5.61 | -4.73 | 4.86 | -2.67 | 2.46 | -7.67 |
Golgi | -2.02 | 6.42 | -0.20 | 0.84 | -1.88 | -3.44 | 5.11 | -1.96 | 1.02 | -3.65 | -4.12 | 6.25 | -1.60 | 1.36 | -3.78 |
Lipid Particles | 14.81 | 20.96 | 20.90 | 20.43 | -1.69 | -1.14 | 21.17 | 20.87 | 21.52 | -1.88 | 7.14 | 27.08 | 26.84 | 29.70 | -2.58 |
Mitochondria | 0.77 | 3.37 | 2.54 | 3.90 | -2.55 | -3.27 | 8.25 | 1.15 | 3.60 | -3.78 | -1.71 | 8.75 | 3.39 | 5.39 | -4.56 |
Mitotic Spindle | -9.78 | -8.44 | -34.64* | -30.10* | -10.55 | -8.82 | -3.58 | -27.12* | -22.06* | -14.09 | -10.71 | -8.31 | -42.42 | -36.70* | -16.47 |
None | 1.71 | 3.89 | 3.49 | 4.73 | -4.81 | -0.27 | 7.96 | 3.03 | 2.76 | -4.55 | 0.84 | 8.81 | 5.78 | 5.23 | -5.91 |
Nuclear Periphery | -3.27 | -11.27 | -16.70 | -16.15 | 3.29 | 2.41 | -5.70 | -6.50 | -8.85 | 2.63 | 3.35 | -10.35 | -12.56 | -17.42 | 4.23 |
Nuclear Periphery Foci | -10.63 | 0.52 | 6.82 | 27.74* | 5.46 | 3.79 | 3.78 | 20.99* | 17.48 | 7.56 | -2.50 | 6.47 | 25.55 | 31.51* | 9.01 |
Nucleolus | 1.38 | -2.96 | 2.53 | 2.72 | 4.90 | -0.29 | -2.08 | 4.25 | 4.63 | 3.85 | 2.15 | -3.11 | 6.58 | 5.61 | 6.19 |
Nucleus | -1.77 | -5.36 | -5.05 | -3.89 | 3.34 | 1.02 | -4.24 | -4.26 | -5.10 | 2.59 | 1.53 | -5.95 | -4.49 | -6.28 | 4.11 |
Peroxisomes | 3.12 | 3.26 | 4.19 | 5.64 | 5.45 | -3.52 | -0.06 | 1.50 | 4.11 | 1.12 | 0.28 | 2.36 | 5.31 | 6.69 | 4.38 |
Vacuole | -3.54 | -4.12 | -8.08 | -6.03 | -5.90 | -1.92 | -1.26 | -7.27 | -6.53 | -5.67 | -2.86 | -2.59 | -10.34 | -8.90 | -8.19 |
Vacuole Periphery | -3.04 | -1.27 | -4.00 | -1.35 | -2.39 | -2.58 | 3.02 | -3.18 | -1.42 | -3.92 | -3.39 | 0.44 | -4.51 | -1.90 | -4.50 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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