Standard name | |
---|---|
Human Ortholog | |
Description | Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 111 | 104 | 79 | 86 | 49 | 66 | 70 | 105 | – | 93 | 91 | 65 | 78 | 116 | 109 | 10 | – | – | 93 | 79 | 142 | 53 | 53 | 113 |
Bud | 75 | 55 | 79 | 83 | 122 | 103 | 136 | 153 | – | 135 | 98 | 109 | 32 | 56 | 52 | 1 | – | – | 150 | 107 | 130 | 150 | 160 | 238 |
Bud Neck | 7 | 8 | 9 | 6 | 9 | 8 | 9 | 19 | – | 13 | 6 | 8 | 0 | 0 | 0 | 0 | – | – | 85 | 75 | 76 | 105 | 71 | 79 |
Bud Site | 65 | 46 | 78 | 75 | 65 | 74 | 57 | 54 | – | 72 | 44 | 51 | 21 | 21 | 9 | 3 | – | – | – | – | – | – | – | – |
Cell Periphery | 16 | 18 | 17 | 38 | 10 | 18 | 43 | 34 | – | 52 | 41 | 53 | 16 | 30 | 30 | 3 | – | – | 7 | 7 | 4 | 3 | 1 | 5 |
Cytoplasm | 43 | 67 | 76 | 55 | 108 | 117 | 145 | 153 | – | 125 | 79 | 117 | 48 | 70 | 121 | 15 | – | – | 9 | 11 | 12 | 20 | 36 | 71 |
Endoplasmic Reticulum | 8 | 6 | 5 | 8 | 4 | 16 | 13 | 18 | – | 15 | 12 | 10 | 7 | 9 | 22 | 2 | – | – | 2 | 2 | 4 | 3 | 4 | 5 |
Endosome | 24 | 15 | 31 | 12 | 37 | 25 | 17 | 32 | – | 11 | 5 | 16 | 3 | 5 | 5 | 0 | – | – | 3 | 4 | 5 | 9 | 7 | 15 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 2 | 1 | 0 | 0 | – | – | 2 | 0 | 1 | 2 | 3 | 2 |
Mitochondria | 17 | 29 | 29 | 34 | 30 | 93 | 189 | 226 | – | 341 | 277 | 313 | 1 | 0 | 0 | 4 | – | – | 1 | 8 | 7 | 9 | 8 | 11 |
Nucleus | 26 | 3 | 10 | 7 | 14 | 16 | 11 | 13 | – | 7 | 4 | 8 | 2 | 3 | 2 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 3 | – | 6 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 6 | 5 | 8 | 5 | 17 | 19 | 14 | 59 | – | 21 | 13 | 13 | 0 | 0 | 1 | 0 | – | – | 0 | 2 | 0 | 0 | 0 | 0 |
Peroxisomes | 51 | 20 | 82 | 74 | 92 | 96 | 107 | 87 | – | 102 | 39 | 72 | 7 | 18 | 9 | 2 | – | – | 2 | 0 | 8 | 5 | 5 | 6 |
SpindlePole | 56 | 15 | 79 | 64 | 91 | 64 | 81 | 127 | – | 66 | 50 | 49 | 35 | 49 | 25 | 5 | – | – | 8 | 6 | 17 | 8 | 9 | 10 |
Vac/Vac Membrane | 5 | 1 | 5 | 1 | 16 | 6 | 18 | 31 | – | 19 | 8 | 12 | 0 | 0 | 2 | 1 | – | – | 2 | 1 | 2 | 5 | 2 | 10 |
Unique Cell Count | 317 | 260 | 367 | 331 | 439 | 449 | 606 | 762 | 692 | 491 | 612 | 176 | 250 | 287 | 38 | 371 | 311 | 418 | 380 | 366 | 578 | |||
Labelled Cell Count | 511 | 394 | 588 | 548 | 664 | 721 | 911 | 1115 | 1078 | 768 | 897 | 252 | 378 | 387 | 46 | 371 | 311 | 418 | 380 | 366 | 578 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 7.6 | 6.0 | 6.0 | 5.7 | 5.0 | 4.9 | 5.2 | – | 4.6 | 4.6 | 4.7 | 7.9 | 7.8 | 8.0 | 5.2 | – | – | 8.2 | 8.3 | 8.3 |
Std Deviation (1e-4) | 1.4 | 1.5 | 1.3 | 1.5 | 1.6 | 1.1 | 1.2 | 1.1 | – | 1.0 | 1.0 | 1.0 | 1.2 | 1.1 | 1.2 | 0.8 | – | – | 1.5 | 1.6 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.07 | -0.26 | -0.28 | -0.2 | – | -0.39 | -0.37 | -0.34 | 0.4 | 0.38 | 0.42 | -0.2 | – | – | 0.44 | 0.46 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.4 | -4.0 | -2.5 | -4.2 | -3.3 | – | 0 | 0 | 0 | 5.5 | 6.5 | 4.6 | 0.7 | – | – |
Bud | 1.1 | 2.0 | 0.5 | 0.3 | -0.6 | – | 0 | 0 | 0 | -0.9 | 0.3 | -1.1 | -2.8 | – | – |
Bud Neck | -0.6 | -0.4 | -0.7 | -1.1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Site | 0.4 | -2.4 | -1.7 | -5.2 | -6.9 | – | 0 | 0 | 0 | -2.6 | -4.3 | -6.8 | -2.0 | – | – |
Cell Periphery | 3.4 | -1.8 | -0.4 | 1.5 | -0.1 | – | 0 | 0 | 0 | 2.0 | 3.4 | 2.9 | 0 | – | – |
Cytoplasm | -1.4 | 1.3 | 1.8 | 1.2 | -0.2 | – | -1.0 | -1.7 | -0.6 | 1.7 | 2.1 | 5.9 | 2.6 | – | – |
Endoplasmic Reticulum | 1.0 | 0 | 2.0 | 0.9 | 1.1 | – | 0 | 0 | 0 | 0 | 1.8 | 4.0 | 0 | – | – |
Endosome | -2.6 | 0 | -1.6 | -3.9 | -2.9 | – | 0 | 0 | 0 | -3.0 | -3.4 | -3.7 | 0 | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Mitochondria | 1.1 | -0.6 | 5.1 | 8.4 | 8.2 | – | 0 | 0 | 0 | -3.5 | -4.6 | -4.9 | 0 | – | – |
Nucleus | -0.5 | 0.4 | 0.7 | -0.9 | -1.1 | – | -2.1 | -2.2 | -1.6 | 0 | -1.3 | -1.9 | 0 | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nucleolus | -0.7 | 1.4 | 1.6 | 0.1 | 3.7 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Peroxisomes | 0 | -0.5 | -0.3 | -1.8 | -4.8 | – | 0 | 0 | 0 | -5.4 | -5.0 | -7.0 | -2.5 | – | – |
SpindlePole | -0.7 | -0.3 | -2.7 | -3.3 | -2.0 | – | 0 | 0 | 0 | -0.4 | -0.6 | -4.4 | -1.2 | – | – |
Vacuole | 0 | 2.0 | 0 | 1.6 | 2.4 | – | 1.4 | 0.3 | 0.7 | 0 | 0 | 0 | 0 | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.9821 | 2.9654 | 3.0503 | 3.5366 | 3.2649 | 3.3436 | 2.5593 | 2.2668 | 2.2673 | 2.6922 | 2.2509 | 2.6206 | 3.0332 | 2.922 | 2.918 | 2.5953 | 2.7693 | 3.1189 |
Actin | 0.2263 | 0.0799 | 0.1679 | 0.3552 | 0.4453 | 0.1454 | 0.3788 | 0.0137 | 0.1855 | 0.2773 | 0.6764 | 0.1761 | 0.1668 | 0.1544 | 0.1758 | 0.3184 | 0.6105 | 0.1521 |
Bud | 0.0634 | 0.0794 | 0.2928 | 0.0951 | 0.0764 | 0.0168 | 0.0003 | 0.0021 | 0.1602 | 0.0979 | 0.0247 | 0.0072 | 0.0111 | 0.1236 | 0.2944 | 0.128 | 0.0195 | 0.0107 |
Bud Neck | 0.0789 | 0.0118 | 0.0061 | 0.0025 | 0.1013 | 0.6414 | 0.0024 | 0.0051 | 0.0148 | 0.0016 | 0.1 | 0.6802 | 0.103 | 0.0077 | 0.0391 | 0.0025 | 0.0017 | 0.7213 |
Bud Periphery | 0.1006 | 0.0605 | 0.3924 | 0.395 | 0.2388 | 0.0278 | 0.0004 | 0.0013 | 0.3979 | 0.5374 | 0.0793 | 0.0229 | 0.0195 | 0.0553 | 0.3818 | 0.5135 | 0.1042 | 0.0151 |
Bud Site | 0.3089 | 0.5638 | 0.0791 | 0.0257 | 0.0944 | 0.0312 | 0.0017 | 0.6485 | 0.0307 | 0.0055 | 0.0136 | 0.011 | 0.1233 | 0.5437 | 0.0742 | 0.0238 | 0.0048 | 0.0088 |
Cell Periphery | 0.0201 | 0.0054 | 0.0031 | 0.0035 | 0.0023 | 0.004 | 0.0006 | 0.0035 | 0.0036 | 0.0112 | 0.002 | 0.002 | 0.0133 | 0.0025 | 0.001 | 0.0049 | 0.0073 | 0.0035 |
Cytoplasm | 0.104 | 0.1381 | 0.0218 | 0.0719 | 0.012 | 0.0428 | 0.0062 | 0.0219 | 0.0847 | 0.0541 | 0.0041 | 0.0153 | 0.3127 | 0.0279 | 0.0031 | 0.0043 | 0.0921 | 0.0351 |
Cytoplasmic Foci | 0.0379 | 0.018 | 0.0096 | 0.0224 | 0.014 | 0.0501 | 0.0218 | 0.1611 | 0.0331 | 0.0011 | 0.0437 | 0.0445 | 0.02 | 0.0037 | 0.0016 | 0.0005 | 0.0505 | 0.0389 |
Eisosomes | 0.0016 | 0.0002 | 0.0003 | 0.0004 | 0.0004 | 0.0004 | 0.0143 | 0.0001 | 0.0006 | 0.0004 | 0.0012 | 0.0006 | 0.0029 | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.0002 |
Endoplasmic Reticulum | 0.0039 | 0.0018 | 0.0006 | 0.0014 | 0.0004 | 0.0012 | 0.0029 | 0.0004 | 0.0014 | 0.004 | 0.0002 | 0.0001 | 0.0153 | 0.0003 | 0.0001 | 0.0003 | 0.0011 | 0.0004 |
Endosome | 0.0091 | 0.0051 | 0.0006 | 0.004 | 0.0008 | 0.0081 | 0.0079 | 0.0351 | 0.0022 | 0.0006 | 0.0021 | 0.0003 | 0.0646 | 0.0001 | 0.0005 | 0.0002 | 0.0213 | 0.0005 |
Golgi | 0.0046 | 0.0037 | 0.0013 | 0.0041 | 0.0025 | 0.0066 | 0.0122 | 0.0183 | 0.0043 | 0.001 | 0.0071 | 0.0011 | 0.0465 | 0.0003 | 0.0013 | 0.0009 | 0.0247 | 0.0015 |
Lipid Particles | 0.0034 | 0.0013 | 0.0007 | 0.0007 | 0.0006 | 0.0037 | 0.0199 | 0.0015 | 0.0014 | 0.0004 | 0.0143 | 0.0005 | 0.005 | 0.0001 | 0.0001 | 0.0001 | 0.0015 | 0.0007 |
Mitochondria | 0.002 | 0.001 | 0.0014 | 0.0018 | 0.001 | 0.0011 | 0.0029 | 0.0027 | 0.0525 | 0.0011 | 0.0014 | 0.0002 | 0.0199 | 0.0001 | 0.0246 | 0.0005 | 0.0356 | 0.0002 |
None | 0.0124 | 0.017 | 0.0171 | 0.0041 | 0.0007 | 0.0019 | 0.4522 | 0.0023 | 0.0183 | 0.0029 | 0.0014 | 0.0347 | 0.0101 | 0.0781 | 0.0007 | 0.0004 | 0.0132 | 0.0038 |
Nuclear Periphery | 0.0016 | 0.0006 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0067 | 0.0002 | 0.0006 | 0.0007 | 0.0001 | 0 | 0.0361 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 |
Nucleolus | 0.001 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0005 | 0.0035 | 0.0001 | 0.0004 | 0.0001 | 0.0022 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0003 |
Nucleus | 0.0013 | 0.0015 | 0.0005 | 0.0005 | 0.0003 | 0.0004 | 0.002 | 0.0004 | 0.0007 | 0.0004 | 0.0003 | 0.0002 | 0.0048 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0002 |
Peroxisomes | 0.0098 | 0.005 | 0.0025 | 0.0071 | 0.0061 | 0.0106 | 0.0443 | 0.0778 | 0.0022 | 0.0003 | 0.0244 | 0.0018 | 0.0031 | 0.0006 | 0.0006 | 0.0002 | 0.003 | 0.0057 |
Punctate Nuclear | 0.0047 | 0.0022 | 0.0005 | 0.0006 | 0.0014 | 0.0026 | 0.0178 | 0.0032 | 0.003 | 0.0002 | 0.0011 | 0.001 | 0.0046 | 0.0002 | 0.0001 | 0.0001 | 0.0021 | 0.0004 |
Vacuole | 0.0041 | 0.003 | 0.0009 | 0.0029 | 0.0008 | 0.0026 | 0.0005 | 0.0004 | 0.0016 | 0.0018 | 0.0005 | 0.0002 | 0.01 | 0.0004 | 0.0003 | 0.0004 | 0.0043 | 0.0005 |
Vacuole Periphery | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0006 | 0.0008 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0064 | 0 | 0.0001 | 0.0001 | 0.0009 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.5143 | 11.501 | 11.2953 | 18.0177 | 20.1149 | 9.8329 | 14.9633 | 22.3914 | 15.303 | 19.8559 |
Translational Efficiency | 0.9986 | 1.0121 | 1.1434 | 0.6677 | 0.6329 | 1.4219 | 0.8596 | 0.5862 | 0.916 | 0.767 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1292 | 1370 | 262 | 1183 | 1035 | 577 | 1901 | 2013 | 2327 | 1947 | 2163 | 3196 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 875.84 | 863.36 | 1069.85 | 1023.70 | 694.64 | 897.80 | 915.56 | 1013.97 | 795.25 | 873.57 | 934.25 | 1017.57 |
Standard Deviation | 112.12 | 145.92 | 136.57 | 147.69 | 159.12 | 124.53 | 113.90 | 139.82 | 162.32 | 140.81 | 127.26 | 142.86 |
Intensity Change Log 2 | — | -0.020705 | 0.288669 | 0.225054 | — | 0.370129 | 0.398389 | 0.545678 | — | 0.165320 | 0.338231 | 0.375715 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.045404 | 0.099345 | 0.291598* | 0.181968* | 0.053967 | 0.086621 | 0.063766 | 0.076839 | 0.049213 | 0.095574 | 0.091363 | 0.115753* |
Bud Neck | 0.094054 | 0.099859 | 0.049732 | 0.039166 | 0.158808 | 0.116323 | 0.085408 | 0.057105 | 0.122855 | 0.104738 | 0.081087 | 0.050465 |
Bud Site | 0.670359* | 0.548792* | 0.511397* | 0.611347* | 0.478153* | 0.570298* | 0.683533* | 0.699938* | 0.584870* | 0.555165* | 0.662683* | 0.667146* |
Cell Periphery | 0.004380 | 0.009943 | 0.009795 | 0.018317 | 0.047456 | 0.028060 | 0.004652 | 0.011318 | 0.023539 | 0.015312 | 0.005275 | 0.013909 |
Cytoplasm | 0.101466* | 0.098453 | 0.045953 | 0.094375 | 0.104347* | 0.083438 | 0.122005* | 0.130690* | 0.102747* | 0.094003 | 0.112793* | 0.117248* |
Cytoplasmic Foci | 0.017606 | 0.052858 | 0.016818 | 0.008069 | 0.049675 | 0.032957 | 0.014402 | 0.004556 | 0.031870 | 0.046961 | 0.014694 | 0.005856 |
Eisosomes | 0.000783 | 0.001139 | 0.002011 | 0.007309 | 0.006165 | 0.005711 | 0.000632 | 0.001348 | 0.003177 | 0.002494 | 0.000799 | 0.003555 |
Endoplasmic Reticulum | 0.000472 | 0.000935 | 0.002827 | 0.001507 | 0.002647 | 0.000649 | 0.000293 | 0.000166 | 0.001440 | 0.000850 | 0.000600 | 0.000662 |
Endosome | 0.007853 | 0.016782 | 0.006976 | 0.002894 | 0.013252 | 0.010842 | 0.002301 | 0.000926 | 0.010254 | 0.015022 | 0.002868 | 0.001655 |
Golgi | 0.004414 | 0.016896 | 0.005422 | 0.002292 | 0.013593 | 0.013766 | 0.001761 | 0.000688 | 0.008497 | 0.015968 | 0.002204 | 0.001282 |
Lipid Particles | 0.001466 | 0.003516 | 0.003373 | 0.000564 | 0.003512 | 0.002682 | 0.000227 | 0.000098 | 0.002376 | 0.003269 | 0.000608 | 0.000270 |
Mitochondria | 0.004925 | 0.007191 | 0.000775 | 0.003223 | 0.026387 | 0.011117 | 0.001809 | 0.001020 | 0.014471 | 0.008354 | 0.001684 | 0.001835 |
Mitotic Spindle | 0.003200 | 0.002344 | 0.004860 | 0.002894 | 0.000587 | 0.001577 | 0.001910 | 0.002210 | 0.002038 | 0.002116 | 0.002268 | 0.002463 |
None | 0.000280 | 0.000536 | 0.000040 | 0.000054 | 0.001677 | 0.000165 | 0.000118 | 0.000106 | 0.000901 | 0.000426 | 0.000108 | 0.000087 |
Nuclear Periphery | 0.000024 | 0.000008 | 0.000035 | 0.000023 | 0.000052 | 0.000019 | 0.000007 | 0.000011 | 0.000037 | 0.000012 | 0.000010 | 0.000016 |
Nuclear Periphery Foci | 0.000177 | 0.000243 | 0.000959 | 0.000206 | 0.000350 | 0.000268 | 0.000102 | 0.000039 | 0.000254 | 0.000251 | 0.000206 | 0.000101 |
Nucleolus | 0.000074 | 0.000074 | 0.000050 | 0.000070 | 0.000262 | 0.000061 | 0.000037 | 0.000021 | 0.000157 | 0.000070 | 0.000039 | 0.000039 |
Nucleus | 0.003423 | 0.000766 | 0.002217 | 0.001072 | 0.004477 | 0.000916 | 0.000742 | 0.000911 | 0.003892 | 0.000810 | 0.000921 | 0.000971 |
Peroxisomes | 0.016779 | 0.032774 | 0.020354 | 0.005642 | 0.024633 | 0.024123 | 0.009521 | 0.003205 | 0.020272 | 0.030210 | 0.010833 | 0.004107 |
Vacuole | 0.021095 | 0.006961 | 0.024339 | 0.017945 | 0.007685 | 0.009219 | 0.006341 | 0.008477 | 0.015130 | 0.007630 | 0.008521 | 0.011982 |
Vacuole Periphery | 0.001766 | 0.000585 | 0.000468 | 0.001061 | 0.002315 | 0.001186 | 0.000434 | 0.000329 | 0.002010 | 0.000763 | 0.000438 | 0.000600 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -7.55 | -10.70 | -13.75 | -7.34 | 4.50 | -3.50 | -1.56 | -3.26 | 1.22 | -1.87 | -8.18 | -7.29 | -11.85 | -2.59 | -3.50 |
Bud Neck | -0.91 | 5.21 | 9.72 | 10.80 | 1.34 | 3.92 | 9.26 | 13.48 | 7.00 | 6.42 | 2.93 | 7.81 | 15.53 | 11.70 | 8.11 |
Bud Site | 9.18 | 7.10 | 5.41 | -3.24 | -3.94 | -5.13 | -16.50 | -16.90 | -7.29 | -0.79 | 2.66 | -7.82 | -7.62 | -10.08 | 0.62 |
Cell Periphery | -4.65 | -2.50 | -6.42 | -3.44 | -2.92 | 3.54 | 10.15 | 8.30 | 4.46 | -4.33 | 3.53 | 9.04 | 4.50 | 1.08 | -6.52 |
Cytoplasm | 0.76 | 5.08 | -0.14 | -0.88 | -5.13 | 2.40 | -2.91 | -4.89 | -6.62 | -2.07 | 1.80 | -2.09 | -4.26 | -5.90 | -1.79 |
Cytoplasmic Foci | -9.76 | -0.10 | 4.84 | 13.10 | 3.54 | 3.31 | 9.22 | 12.43 | 7.54 | 6.02 | -4.84 | 7.53 | 13.31 | 15.73 | 6.18 |
Eisosomes | -1.51 | -2.54 | -5.38 | -5.09 | -4.16 | 0.31 | 7.04 | 6.12 | 3.45 | -2.79 | 1.28 | 6.11 | -0.43 | -1.57 | -5.65 |
Endoplasmic Reticulum | -1.47 | -2.55 | -2.20 | -1.09 | 1.42 | 3.40 | 4.06 | 4.32 | 3.43 | 1.64 | 1.77 | 2.65 | 2.47 | 0.73 | -0.13 |
Endosome | -4.99 | 0.35 | 3.92 | 9.13 | 2.90 | 1.29 | 8.36 | 9.72 | 7.17 | 3.22 | -3.53 | 8.03 | 9.85 | 12.18 | 2.74 |
Golgi | -6.82 | -0.57 | 2.01 | 8.39 | 2.08 | -0.05 | 7.87 | 8.66 | 5.45 | 4.01 | -4.75 | 7.31 | 8.42 | 10.77 | 2.54 |
Lipid Particles | -2.15 | -1.06 | 2.15 | 3.25 | 1.51 | 0.50 | 5.87 | 6.12 | 1.62 | 1.65 | -1.06 | 4.29 | 6.36 | 3.80 | 1.35 |
Mitochondria | -1.46 | 3.34 | 1.11 | 2.79 | -2.15 | 4.02 | 9.59 | 9.97 | 3.55 | 1.83 | 3.53 | 9.32 | 9.08 | 5.57 | -0.21 |
Mitotic Spindle | 0.95 | -0.73 | 0.23 | -0.55 | 0.80 | -2.16 | -4.11 | -3.59 | -1.33 | -0.92 | -0.14 | -0.51 | -1.03 | -0.85 | -0.51 |
None | -1.14 | 2.25 | 2.14 | 2.42 | -1.02 | 2.52 | 2.61 | 2.61 | 0.94 | 0.21 | 1.55 | 2.91 | 2.97 | 2.37 | 0.88 |
Nuclear Periphery | 3.36 | -1.43 | -0.86 | -3.94 | 1.15 | 1.16 | 1.62 | 1.23 | 0.12 | -2.33 | 1.98 | 1.80 | 1.08 | -2.67 | -2.00 |
Nuclear Periphery Foci | -0.96 | -3.13 | -0.82 | 0.02 | 2.93 | 0.93 | 2.99 | 4.36 | 3.48 | 1.85 | 0.10 | -0.22 | 3.04 | 2.59 | 2.42 |
Nucleolus | 0.02 | 0.21 | -0.33 | -0.34 | -0.41 | 2.28 | 2.34 | 2.73 | 2.50 | 1.03 | 2.10 | 2.28 | 2.14 | 0.59 | -0.01 |
Nucleus | 3.94 | -0.20 | 2.76 | -4.45 | 2.40 | 2.53 | 2.60 | 2.21 | -1.85 | -2.51 | 4.28 | 3.70 | 3.39 | -4.23 | -1.25 |
Peroxisomes | -5.43 | -0.66 | 5.60 | 11.05 | 3.31 | 0.11 | 5.73 | 8.60 | 5.46 | 6.05 | -4.00 | 5.33 | 10.49 | 12.93 | 6.27 |
Vacuole | 6.45 | -2.05 | -2.48 | -7.28 | 0.68 | -0.75 | -0.08 | -3.52 | -2.20 | -3.66 | 5.30 | 2.42 | -2.50 | -7.58 | -4.80 |
Vacuole Periphery | 1.64 | 1.69 | 0.83 | -0.89 | -0.98 | 1.67 | 3.01 | 3.37 | 2.17 | 0.34 | 2.57 | 3.01 | 2.77 | 0.70 | -0.50 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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