Standard name
Human Ortholog
Description Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.35 0.4 0.22 0.26 0.11 0.15 0.12 0.14 0.13 0.19 0.11 0.44 0.46 0.38 0.26 0.25 0.26 0.34 0.14 0.15 0.2
Bud 0.24 0.21 0.22 0.25 0.28 0.23 0.22 0.2 0.2 0.2 0.18 0.18 0.22 0.18 0 0.41 0.35 0.31 0.4 0.44 0.41
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.23 0.24 0.18 0.28 0.19 0.14
Bud Site 0.21 0.18 0.21 0.23 0.15 0.16 0.09 0.07 0.1 0.09 0.08 0.12 0.08 0 0.08
Cell Periphery 0.05 0.07 0.05 0.11 0 0 0.07 0 0.08 0.08 0.09 0.09 0.12 0.1 0.08 0 0 0 0 0 0
Cytoplasm 0.14 0.26 0.21 0.17 0.25 0.26 0.24 0.2 0.18 0.16 0.19 0.27 0.28 0.42 0.39 0 0 0 0.05 0.1 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0 0 0 0
Endosome 0.08 0.06 0.08 0 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.11 0.08 0.1 0.07 0.21 0.31 0.3 0.49 0.56 0.51 0 0 0 0.11 0 0 0 0 0 0
Nucleus 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.16 0.08 0.22 0.22 0.21 0.21 0.18 0.11 0.15 0.08 0.12 0 0.07 0 0.05 0 0 0 0 0 0
SpindlePole 0.18 0.06 0.22 0.19 0.21 0.14 0.13 0.17 0.1 0.1 0.08 0.2 0.2 0.09 0.13 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 111 104 79 86 49 66 70 105 93 91 65 78 116 109 10 93 79 142 53 53 113
Bud 75 55 79 83 122 103 136 153 135 98 109 32 56 52 1 150 107 130 150 160 238
Bud Neck 7 8 9 6 9 8 9 19 13 6 8 0 0 0 0 85 75 76 105 71 79
Bud Site 65 46 78 75 65 74 57 54 72 44 51 21 21 9 3
Cell Periphery 16 18 17 38 10 18 43 34 52 41 53 16 30 30 3 7 7 4 3 1 5
Cytoplasm 43 67 76 55 108 117 145 153 125 79 117 48 70 121 15 9 11 12 20 36 71
Endoplasmic Reticulum 8 6 5 8 4 16 13 18 15 12 10 7 9 22 2 2 2 4 3 4 5
Endosome 24 15 31 12 37 25 17 32 11 5 16 3 5 5 0 3 4 5 9 7 15
Golgi 1 0 0 0 0 0 0 1 0 0 0 2 1 0 0 2 0 1 2 3 2
Mitochondria 17 29 29 34 30 93 189 226 341 277 313 1 0 0 4 1 8 7 9 8 11
Nucleus 26 3 10 7 14 16 11 13 7 4 8 2 3 2 0 0 0 0 0 0 0
Nuclear Periphery 0 2 1 0 0 0 1 3 6 1 1 0 0 0 0 0 0 0 0 0 2
Nucleolus 6 5 8 5 17 19 14 59 21 13 13 0 0 1 0 0 2 0 0 0 0
Peroxisomes 51 20 82 74 92 96 107 87 102 39 72 7 18 9 2 2 0 8 5 5 6
SpindlePole 56 15 79 64 91 64 81 127 66 50 49 35 49 25 5 8 6 17 8 9 10
Vac/Vac Membrane 5 1 5 1 16 6 18 31 19 8 12 0 0 2 1 2 1 2 5 2 10
Unique Cell Count 317 260 367 331 439 449 606 762 692 491 612 176 250 287 38 371 311 418 380 366 578
Labelled Cell Count 511 394 588 548 664 721 911 1115 1078 768 897 252 378 387 46 371 311 418 380 366 578


Ambiguous, Bud, Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 7.6 6.0 6.0 5.7 5.0 4.9 5.2 4.6 4.6 4.7 7.9 7.8 8.0 5.2 8.2 8.3 8.3
Std Deviation (1e-4) 1.4 1.5 1.3 1.5 1.6 1.1 1.2 1.1 1.0 1.0 1.0 1.2 1.1 1.2 0.8 1.5 1.6 1.4
Intensity Change (Log2) 0.01 -0.07 -0.26 -0.28 -0.2 -0.39 -0.37 -0.34 0.4 0.38 0.42 -0.2 0.44 0.46 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.4 -4.0 -2.5 -4.2 -3.3 0 0 0 5.5 6.5 4.6 0.7
Bud 1.1 2.0 0.5 0.3 -0.6 0 0 0 -0.9 0.3 -1.1 -2.8
Bud Neck -0.6 -0.4 -0.7 -1.1 0 0 0 0 0 0 0 0
Bud Site 0.4 -2.4 -1.7 -5.2 -6.9 0 0 0 -2.6 -4.3 -6.8 -2.0
Cell Periphery 3.4 -1.8 -0.4 1.5 -0.1 0 0 0 2.0 3.4 2.9 0
Cytoplasm -1.4 1.3 1.8 1.2 -0.2 -1.0 -1.7 -0.6 1.7 2.1 5.9 2.6
Endoplasmic Reticulum 1.0 0 2.0 0.9 1.1 0 0 0 0 1.8 4.0 0
Endosome -2.6 0 -1.6 -3.9 -2.9 0 0 0 -3.0 -3.4 -3.7 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.1 -0.6 5.1 8.4 8.2 0 0 0 -3.5 -4.6 -4.9 0
Nucleus -0.5 0.4 0.7 -0.9 -1.1 -2.1 -2.2 -1.6 0 -1.3 -1.9 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.7 1.4 1.6 0.1 3.7 0 0 0 0 0 0 0
Peroxisomes 0 -0.5 -0.3 -1.8 -4.8 0 0 0 -5.4 -5.0 -7.0 -2.5
SpindlePole -0.7 -0.3 -2.7 -3.3 -2.0 0 0 0 -0.4 -0.6 -4.4 -1.2
Vacuole 0 2.0 0 1.6 2.4 1.4 0.3 0.7 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9821 2.9654 3.0503 3.5366 3.2649 3.3436 2.5593 2.2668 2.2673 2.6922 2.2509 2.6206 3.0332 2.922 2.918 2.5953 2.7693 3.1189
Actin 0.2263 0.0799 0.1679 0.3552 0.4453 0.1454 0.3788 0.0137 0.1855 0.2773 0.6764 0.1761 0.1668 0.1544 0.1758 0.3184 0.6105 0.1521
Bud 0.0634 0.0794 0.2928 0.0951 0.0764 0.0168 0.0003 0.0021 0.1602 0.0979 0.0247 0.0072 0.0111 0.1236 0.2944 0.128 0.0195 0.0107
Bud Neck 0.0789 0.0118 0.0061 0.0025 0.1013 0.6414 0.0024 0.0051 0.0148 0.0016 0.1 0.6802 0.103 0.0077 0.0391 0.0025 0.0017 0.7213
Bud Periphery 0.1006 0.0605 0.3924 0.395 0.2388 0.0278 0.0004 0.0013 0.3979 0.5374 0.0793 0.0229 0.0195 0.0553 0.3818 0.5135 0.1042 0.0151
Bud Site 0.3089 0.5638 0.0791 0.0257 0.0944 0.0312 0.0017 0.6485 0.0307 0.0055 0.0136 0.011 0.1233 0.5437 0.0742 0.0238 0.0048 0.0088
Cell Periphery 0.0201 0.0054 0.0031 0.0035 0.0023 0.004 0.0006 0.0035 0.0036 0.0112 0.002 0.002 0.0133 0.0025 0.001 0.0049 0.0073 0.0035
Cytoplasm 0.104 0.1381 0.0218 0.0719 0.012 0.0428 0.0062 0.0219 0.0847 0.0541 0.0041 0.0153 0.3127 0.0279 0.0031 0.0043 0.0921 0.0351
Cytoplasmic Foci 0.0379 0.018 0.0096 0.0224 0.014 0.0501 0.0218 0.1611 0.0331 0.0011 0.0437 0.0445 0.02 0.0037 0.0016 0.0005 0.0505 0.0389
Eisosomes 0.0016 0.0002 0.0003 0.0004 0.0004 0.0004 0.0143 0.0001 0.0006 0.0004 0.0012 0.0006 0.0029 0.0002 0.0002 0.0005 0.0006 0.0002
Endoplasmic Reticulum 0.0039 0.0018 0.0006 0.0014 0.0004 0.0012 0.0029 0.0004 0.0014 0.004 0.0002 0.0001 0.0153 0.0003 0.0001 0.0003 0.0011 0.0004
Endosome 0.0091 0.0051 0.0006 0.004 0.0008 0.0081 0.0079 0.0351 0.0022 0.0006 0.0021 0.0003 0.0646 0.0001 0.0005 0.0002 0.0213 0.0005
Golgi 0.0046 0.0037 0.0013 0.0041 0.0025 0.0066 0.0122 0.0183 0.0043 0.001 0.0071 0.0011 0.0465 0.0003 0.0013 0.0009 0.0247 0.0015
Lipid Particles 0.0034 0.0013 0.0007 0.0007 0.0006 0.0037 0.0199 0.0015 0.0014 0.0004 0.0143 0.0005 0.005 0.0001 0.0001 0.0001 0.0015 0.0007
Mitochondria 0.002 0.001 0.0014 0.0018 0.001 0.0011 0.0029 0.0027 0.0525 0.0011 0.0014 0.0002 0.0199 0.0001 0.0246 0.0005 0.0356 0.0002
None 0.0124 0.017 0.0171 0.0041 0.0007 0.0019 0.4522 0.0023 0.0183 0.0029 0.0014 0.0347 0.0101 0.0781 0.0007 0.0004 0.0132 0.0038
Nuclear Periphery 0.0016 0.0006 0.0003 0.0004 0.0002 0.0002 0.0067 0.0002 0.0006 0.0007 0.0001 0 0.0361 0.0001 0.0001 0.0001 0.0004 0
Nucleolus 0.001 0.0005 0.0002 0.0003 0.0002 0.0005 0.0035 0.0001 0.0004 0.0001 0.0022 0.0001 0.0009 0.0001 0.0001 0 0.0002 0.0003
Nucleus 0.0013 0.0015 0.0005 0.0005 0.0003 0.0004 0.002 0.0004 0.0007 0.0004 0.0003 0.0002 0.0048 0.0003 0.0002 0.0002 0.0003 0.0002
Peroxisomes 0.0098 0.005 0.0025 0.0071 0.0061 0.0106 0.0443 0.0778 0.0022 0.0003 0.0244 0.0018 0.0031 0.0006 0.0006 0.0002 0.003 0.0057
Punctate Nuclear 0.0047 0.0022 0.0005 0.0006 0.0014 0.0026 0.0178 0.0032 0.003 0.0002 0.0011 0.001 0.0046 0.0002 0.0001 0.0001 0.0021 0.0004
Vacuole 0.0041 0.003 0.0009 0.0029 0.0008 0.0026 0.0005 0.0004 0.0016 0.0018 0.0005 0.0002 0.01 0.0004 0.0003 0.0004 0.0043 0.0005
Vacuole Periphery 0.0003 0.0003 0.0001 0.0003 0.0001 0.0006 0.0008 0.0001 0.0003 0.0002 0.0001 0 0.0064 0 0.0001 0.0001 0.0009 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.5143 11.501 11.2953 18.0177 20.1149 9.8329 14.9633 22.3914 15.303 19.8559
Translational Efficiency 0.9986 1.0121 1.1434 0.6677 0.6329 1.4219 0.8596 0.5862 0.916 0.767

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
Localization
Cell Percentages
Cell Cycle Regulation No
Subcompartmental Group N/A

Sec5

Sec5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available