Standard name | |
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Human Ortholog | |
Description | Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 111 | 104 | 79 | 86 | 49 | 66 | 70 | 105 | – | 93 | 91 | 65 | 78 | 116 | 109 | 10 | – | – | 93 | 79 | 142 | 53 | 53 | 113 |
Bud | 75 | 55 | 79 | 83 | 122 | 103 | 136 | 153 | – | 135 | 98 | 109 | 32 | 56 | 52 | 1 | – | – | 150 | 107 | 130 | 150 | 160 | 238 |
Bud Neck | 7 | 8 | 9 | 6 | 9 | 8 | 9 | 19 | – | 13 | 6 | 8 | 0 | 0 | 0 | 0 | – | – | 85 | 75 | 76 | 105 | 71 | 79 |
Bud Site | 65 | 46 | 78 | 75 | 65 | 74 | 57 | 54 | – | 72 | 44 | 51 | 21 | 21 | 9 | 3 | – | – | – | – | – | – | – | – |
Cell Periphery | 16 | 18 | 17 | 38 | 10 | 18 | 43 | 34 | – | 52 | 41 | 53 | 16 | 30 | 30 | 3 | – | – | 7 | 7 | 4 | 3 | 1 | 5 |
Cytoplasm | 43 | 67 | 76 | 55 | 108 | 117 | 145 | 153 | – | 125 | 79 | 117 | 48 | 70 | 121 | 15 | – | – | 9 | 11 | 12 | 20 | 36 | 71 |
Endoplasmic Reticulum | 8 | 6 | 5 | 8 | 4 | 16 | 13 | 18 | – | 15 | 12 | 10 | 7 | 9 | 22 | 2 | – | – | 2 | 2 | 4 | 3 | 4 | 5 |
Endosome | 24 | 15 | 31 | 12 | 37 | 25 | 17 | 32 | – | 11 | 5 | 16 | 3 | 5 | 5 | 0 | – | – | 3 | 4 | 5 | 9 | 7 | 15 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 2 | 1 | 0 | 0 | – | – | 2 | 0 | 1 | 2 | 3 | 2 |
Mitochondria | 17 | 29 | 29 | 34 | 30 | 93 | 189 | 226 | – | 341 | 277 | 313 | 1 | 0 | 0 | 4 | – | – | 1 | 8 | 7 | 9 | 8 | 11 |
Nucleus | 26 | 3 | 10 | 7 | 14 | 16 | 11 | 13 | – | 7 | 4 | 8 | 2 | 3 | 2 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 3 | – | 6 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 6 | 5 | 8 | 5 | 17 | 19 | 14 | 59 | – | 21 | 13 | 13 | 0 | 0 | 1 | 0 | – | – | 0 | 2 | 0 | 0 | 0 | 0 |
Peroxisomes | 51 | 20 | 82 | 74 | 92 | 96 | 107 | 87 | – | 102 | 39 | 72 | 7 | 18 | 9 | 2 | – | – | 2 | 0 | 8 | 5 | 5 | 6 |
SpindlePole | 56 | 15 | 79 | 64 | 91 | 64 | 81 | 127 | – | 66 | 50 | 49 | 35 | 49 | 25 | 5 | – | – | 8 | 6 | 17 | 8 | 9 | 10 |
Vac/Vac Membrane | 5 | 1 | 5 | 1 | 16 | 6 | 18 | 31 | – | 19 | 8 | 12 | 0 | 0 | 2 | 1 | – | – | 2 | 1 | 2 | 5 | 2 | 10 |
Unique Cell Count | 317 | 260 | 367 | 331 | 439 | 449 | 606 | 762 | 692 | 491 | 612 | 176 | 250 | 287 | 38 | 371 | 311 | 418 | 380 | 366 | 578 | |||
Labelled Cell Count | 511 | 394 | 588 | 548 | 664 | 721 | 911 | 1115 | 1078 | 768 | 897 | 252 | 378 | 387 | 46 | 371 | 311 | 418 | 380 | 366 | 578 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 7.6 | 6.0 | 6.0 | 5.7 | 5.0 | 4.9 | 5.2 | – | 4.6 | 4.6 | 4.7 | 7.9 | 7.8 | 8.0 | 5.2 | – | – | 8.2 | 8.3 | 8.3 |
Std Deviation (1e-4) | 1.4 | 1.5 | 1.3 | 1.5 | 1.6 | 1.1 | 1.2 | 1.1 | – | 1.0 | 1.0 | 1.0 | 1.2 | 1.1 | 1.2 | 0.8 | – | – | 1.5 | 1.6 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.07 | -0.26 | -0.28 | -0.2 | – | -0.39 | -0.37 | -0.34 | 0.4 | 0.38 | 0.42 | -0.2 | – | – | 0.44 | 0.46 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.4 | -4.0 | -2.5 | -4.2 | -3.3 | – | 0 | 0 | 0 | 5.5 | 6.5 | 4.6 | 0.7 | – | – |
Bud | 1.1 | 2.0 | 0.5 | 0.3 | -0.6 | – | 0 | 0 | 0 | -0.9 | 0.3 | -1.1 | -2.8 | – | – |
Bud Neck | -0.6 | -0.4 | -0.7 | -1.1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Site | 0.4 | -2.4 | -1.7 | -5.2 | -6.9 | – | 0 | 0 | 0 | -2.6 | -4.3 | -6.8 | -2.0 | – | – |
Cell Periphery | 3.4 | -1.8 | -0.4 | 1.5 | -0.1 | – | 0 | 0 | 0 | 2.0 | 3.4 | 2.9 | 0 | – | – |
Cytoplasm | -1.4 | 1.3 | 1.8 | 1.2 | -0.2 | – | -1.0 | -1.7 | -0.6 | 1.7 | 2.1 | 5.9 | 2.6 | – | – |
Endoplasmic Reticulum | 1.0 | 0 | 2.0 | 0.9 | 1.1 | – | 0 | 0 | 0 | 0 | 1.8 | 4.0 | 0 | – | – |
Endosome | -2.6 | 0 | -1.6 | -3.9 | -2.9 | – | 0 | 0 | 0 | -3.0 | -3.4 | -3.7 | 0 | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Mitochondria | 1.1 | -0.6 | 5.1 | 8.4 | 8.2 | – | 0 | 0 | 0 | -3.5 | -4.6 | -4.9 | 0 | – | – |
Nucleus | -0.5 | 0.4 | 0.7 | -0.9 | -1.1 | – | -2.1 | -2.2 | -1.6 | 0 | -1.3 | -1.9 | 0 | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nucleolus | -0.7 | 1.4 | 1.6 | 0.1 | 3.7 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Peroxisomes | 0 | -0.5 | -0.3 | -1.8 | -4.8 | – | 0 | 0 | 0 | -5.4 | -5.0 | -7.0 | -2.5 | – | – |
SpindlePole | -0.7 | -0.3 | -2.7 | -3.3 | -2.0 | – | 0 | 0 | 0 | -0.4 | -0.6 | -4.4 | -1.2 | – | – |
Vacuole | 0 | 2.0 | 0 | 1.6 | 2.4 | – | 1.4 | 0.3 | 0.7 | 0 | 0 | 0 | 0 | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.9821 | 2.9654 | 3.0503 | 3.5366 | 3.2649 | 3.3436 | 2.5593 | 2.2668 | 2.2673 | 2.6922 | 2.2509 | 2.6206 | 3.0332 | 2.922 | 2.918 | 2.5953 | 2.7693 | 3.1189 |
Actin | 0.2263 | 0.0799 | 0.1679 | 0.3552 | 0.4453 | 0.1454 | 0.3788 | 0.0137 | 0.1855 | 0.2773 | 0.6764 | 0.1761 | 0.1668 | 0.1544 | 0.1758 | 0.3184 | 0.6105 | 0.1521 |
Bud | 0.0634 | 0.0794 | 0.2928 | 0.0951 | 0.0764 | 0.0168 | 0.0003 | 0.0021 | 0.1602 | 0.0979 | 0.0247 | 0.0072 | 0.0111 | 0.1236 | 0.2944 | 0.128 | 0.0195 | 0.0107 |
Bud Neck | 0.0789 | 0.0118 | 0.0061 | 0.0025 | 0.1013 | 0.6414 | 0.0024 | 0.0051 | 0.0148 | 0.0016 | 0.1 | 0.6802 | 0.103 | 0.0077 | 0.0391 | 0.0025 | 0.0017 | 0.7213 |
Bud Periphery | 0.1006 | 0.0605 | 0.3924 | 0.395 | 0.2388 | 0.0278 | 0.0004 | 0.0013 | 0.3979 | 0.5374 | 0.0793 | 0.0229 | 0.0195 | 0.0553 | 0.3818 | 0.5135 | 0.1042 | 0.0151 |
Bud Site | 0.3089 | 0.5638 | 0.0791 | 0.0257 | 0.0944 | 0.0312 | 0.0017 | 0.6485 | 0.0307 | 0.0055 | 0.0136 | 0.011 | 0.1233 | 0.5437 | 0.0742 | 0.0238 | 0.0048 | 0.0088 |
Cell Periphery | 0.0201 | 0.0054 | 0.0031 | 0.0035 | 0.0023 | 0.004 | 0.0006 | 0.0035 | 0.0036 | 0.0112 | 0.002 | 0.002 | 0.0133 | 0.0025 | 0.001 | 0.0049 | 0.0073 | 0.0035 |
Cytoplasm | 0.104 | 0.1381 | 0.0218 | 0.0719 | 0.012 | 0.0428 | 0.0062 | 0.0219 | 0.0847 | 0.0541 | 0.0041 | 0.0153 | 0.3127 | 0.0279 | 0.0031 | 0.0043 | 0.0921 | 0.0351 |
Cytoplasmic Foci | 0.0379 | 0.018 | 0.0096 | 0.0224 | 0.014 | 0.0501 | 0.0218 | 0.1611 | 0.0331 | 0.0011 | 0.0437 | 0.0445 | 0.02 | 0.0037 | 0.0016 | 0.0005 | 0.0505 | 0.0389 |
Eisosomes | 0.0016 | 0.0002 | 0.0003 | 0.0004 | 0.0004 | 0.0004 | 0.0143 | 0.0001 | 0.0006 | 0.0004 | 0.0012 | 0.0006 | 0.0029 | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.0002 |
Endoplasmic Reticulum | 0.0039 | 0.0018 | 0.0006 | 0.0014 | 0.0004 | 0.0012 | 0.0029 | 0.0004 | 0.0014 | 0.004 | 0.0002 | 0.0001 | 0.0153 | 0.0003 | 0.0001 | 0.0003 | 0.0011 | 0.0004 |
Endosome | 0.0091 | 0.0051 | 0.0006 | 0.004 | 0.0008 | 0.0081 | 0.0079 | 0.0351 | 0.0022 | 0.0006 | 0.0021 | 0.0003 | 0.0646 | 0.0001 | 0.0005 | 0.0002 | 0.0213 | 0.0005 |
Golgi | 0.0046 | 0.0037 | 0.0013 | 0.0041 | 0.0025 | 0.0066 | 0.0122 | 0.0183 | 0.0043 | 0.001 | 0.0071 | 0.0011 | 0.0465 | 0.0003 | 0.0013 | 0.0009 | 0.0247 | 0.0015 |
Lipid Particles | 0.0034 | 0.0013 | 0.0007 | 0.0007 | 0.0006 | 0.0037 | 0.0199 | 0.0015 | 0.0014 | 0.0004 | 0.0143 | 0.0005 | 0.005 | 0.0001 | 0.0001 | 0.0001 | 0.0015 | 0.0007 |
Mitochondria | 0.002 | 0.001 | 0.0014 | 0.0018 | 0.001 | 0.0011 | 0.0029 | 0.0027 | 0.0525 | 0.0011 | 0.0014 | 0.0002 | 0.0199 | 0.0001 | 0.0246 | 0.0005 | 0.0356 | 0.0002 |
None | 0.0124 | 0.017 | 0.0171 | 0.0041 | 0.0007 | 0.0019 | 0.4522 | 0.0023 | 0.0183 | 0.0029 | 0.0014 | 0.0347 | 0.0101 | 0.0781 | 0.0007 | 0.0004 | 0.0132 | 0.0038 |
Nuclear Periphery | 0.0016 | 0.0006 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0067 | 0.0002 | 0.0006 | 0.0007 | 0.0001 | 0 | 0.0361 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 |
Nucleolus | 0.001 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0005 | 0.0035 | 0.0001 | 0.0004 | 0.0001 | 0.0022 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0003 |
Nucleus | 0.0013 | 0.0015 | 0.0005 | 0.0005 | 0.0003 | 0.0004 | 0.002 | 0.0004 | 0.0007 | 0.0004 | 0.0003 | 0.0002 | 0.0048 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0002 |
Peroxisomes | 0.0098 | 0.005 | 0.0025 | 0.0071 | 0.0061 | 0.0106 | 0.0443 | 0.0778 | 0.0022 | 0.0003 | 0.0244 | 0.0018 | 0.0031 | 0.0006 | 0.0006 | 0.0002 | 0.003 | 0.0057 |
Punctate Nuclear | 0.0047 | 0.0022 | 0.0005 | 0.0006 | 0.0014 | 0.0026 | 0.0178 | 0.0032 | 0.003 | 0.0002 | 0.0011 | 0.001 | 0.0046 | 0.0002 | 0.0001 | 0.0001 | 0.0021 | 0.0004 |
Vacuole | 0.0041 | 0.003 | 0.0009 | 0.0029 | 0.0008 | 0.0026 | 0.0005 | 0.0004 | 0.0016 | 0.0018 | 0.0005 | 0.0002 | 0.01 | 0.0004 | 0.0003 | 0.0004 | 0.0043 | 0.0005 |
Vacuole Periphery | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0006 | 0.0008 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0064 | 0 | 0.0001 | 0.0001 | 0.0009 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.5143 | 11.501 | 11.2953 | 18.0177 | 20.1149 | 9.8329 | 14.9633 | 22.3914 | 15.303 | 19.8559 |
Translational Efficiency | 0.9986 | 1.0121 | 1.1434 | 0.6677 | 0.6329 | 1.4219 | 0.8596 | 0.5862 | 0.916 | 0.767 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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