Standard name | |
---|---|
Human Ortholog | |
Description | Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 1 | 2 | 4 | 7 | 9 | 14 | 17 | 15 | 18 | 17 | 30 | 13 | 18 | 12 | 15 | 17 | 20 | 3 | 0 | 4 | 5 | 17 | 19 |
Bud | 12 | 2 | 3 | 4 | 6 | 3 | 12 | 10 | 13 | 10 | 9 | 9 | 9 | 8 | 5 | 0 | 6 | 13 | 8 | 1 | 10 | 5 | 27 | 39 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 2 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 6 | 2 | 4 | 12 | 13 | 25 | 36 | 31 | 12 | 23 | 26 | 36 | 3 | 13 | 6 | 9 | 7 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 354 | 165 | 362 | 290 | 305 | 373 | 367 | 311 | 213 | 250 | 199 | 163 | 337 | 221 | 150 | 135 | 126 | 158 | 371 | 184 | 325 | 278 | 231 | 204 |
Endoplasmic Reticulum | 0 | 3 | 3 | 4 | 5 | 13 | 18 | 22 | 11 | 15 | 21 | 20 | 0 | 2 | 0 | 10 | 6 | 3 | 13 | 3 | 10 | 6 | 12 | 9 |
Endosome | 6 | 0 | 7 | 5 | 7 | 3 | 1 | 1 | 1 | 1 | 2 | 1 | 56 | 82 | 116 | 20 | 18 | 23 | 5 | 3 | 14 | 22 | 80 | 67 |
Golgi | 1 | 1 | 3 | 1 | 7 | 7 | 16 | 4 | 7 | 13 | 8 | 15 | 4 | 19 | 17 | 41 | 31 | 20 | 2 | 0 | 1 | 5 | 30 | 19 |
Mitochondria | 13 | 0 | 6 | 17 | 21 | 47 | 62 | 39 | 68 | 48 | 52 | 25 | 6 | 3 | 1 | 9 | 12 | 17 | 6 | 3 | 20 | 23 | 28 | 23 |
Nucleus | 2 | 2 | 2 | 0 | 0 | 3 | 2 | 3 | 4 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 3 | 1 | 0 | 0 | 0 | 2 | 5 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Nucleolus | 2 | 3 | 4 | 3 | 2 | 1 | 5 | 7 | 7 | 1 | 2 | 2 | 5 | 18 | 32 | 24 | 25 | 17 | 0 | 0 | 1 | 0 | 1 | 2 |
Peroxisomes | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 17 | 26 | 67 | 6 | 6 | 7 | 1 | 0 | 0 | 0 | 18 | 16 |
SpindlePole | 0 | 0 | 6 | 4 | 5 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 35 | 73 | 128 | 45 | 23 | 19 | 7 | 0 | 11 | 9 | 47 | 37 |
Vac/Vac Membrane | 6 | 3 | 26 | 31 | 53 | 74 | 144 | 140 | 102 | 114 | 53 | 104 | 22 | 36 | 24 | 60 | 39 | 33 | 12 | 4 | 9 | 6 | 11 | 10 |
Unique Cell Count | 382 | 176 | 408 | 338 | 387 | 509 | 599 | 531 | 404 | 432 | 330 | 342 | 451 | 415 | 430 | 291 | 246 | 274 | 444 | 209 | 423 | 373 | 521 | 466 |
Labelled Cell Count | 407 | 183 | 429 | 376 | 431 | 561 | 682 | 586 | 453 | 497 | 389 | 406 | 508 | 519 | 563 | 375 | 317 | 337 | 444 | 209 | 423 | 373 | 521 | 466 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.8 | 12.1 | 8.4 | 8.2 | 11.9 | 12.7 | 14.4 | 16.0 | 15.7 | 17.8 | 18.1 | 19.9 | 9.5 | 12.6 | 14.7 | 22.3 | 21.0 | 19.6 | 6.8 | 7.7 | 9.5 |
Std Deviation (1e-4) | 2.3 | 6.1 | 2.5 | 2.8 | 3.7 | 3.9 | 4.9 | 6.4 | 7.0 | 8.7 | 10.4 | 10.9 | 3.2 | 4.1 | 6.2 | 9.6 | 8.8 | 9.3 | 2.3 | 1.8 | 3.1 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.5 | 0.6 | 0.77 | 0.93 | 0.9 | 1.08 | 1.11 | 1.24 | 0.17 | 0.59 | 0.81 | 1.41 | 1.32 | 1.22 | -0.31 | -0.13 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 2.3 | 2.9 | 3.2 | 3.5 | 4.0 | 5.6 | 2.7 | 3.6 | 2.6 | 3.9 | 4.7 | 4.9 |
Bud | 0 | 0 | 0 | 1.6 | 1.5 | 2.5 | 1.9 | 2.1 | 2.1 | 1.6 | 1.5 | 0 | 0 | 0 | 3.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.4 | 2.3 | 3.4 | 4.0 | 3.9 | 2.0 | 3.6 | 4.7 | 5.8 | 0 | 2.2 | 0 | 2.0 | 0 | 0 |
Cytoplasm | -1.2 | -3.8 | -5.8 | -9.6 | -10.2 | -11.3 | -10.1 | -9.0 | -12.2 | -5.3 | -11.2 | -16.0 | -12.2 | -10.7 | -9.3 |
Endoplasmic Reticulum | 0 | 0 | 2.1 | 2.5 | 3.2 | 2.2 | 2.7 | 4.3 | 4.0 | 0 | 0 | 0 | 2.6 | 0 | 0 |
Endosome | -0.3 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.0 | 8.3 | 10.3 | 3.5 | 3.6 | 4.2 |
Golgi | 0 | 0 | 0 | 2.2 | 0 | 0 | 2.4 | 0 | 3.3 | 0 | 3.4 | 3.1 | 7.2 | 6.6 | 4.7 |
Mitochondria | 2.8 | 3.1 | 5.0 | 5.5 | 4.2 | 7.6 | 5.7 | 7.2 | 4.0 | -0.2 | 0 | 0 | 1.5 | 2.6 | 3.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0.9 | 0 | 0 | 0 | 0 | 3.0 | 4.6 | 4.8 | 5.5 | 3.9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 4.8 | 8.1 | 0 | 0 | 0 |
SpindlePole | 0 | -0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.3 | 7.8 | 11.2 | 7.0 | 4.7 | 3.7 |
Vacuole | 1.4 | 3.4 | 3.9 | 7.3 | 8.0 | 7.4 | 7.8 | 4.2 | 8.7 | -1.0 | 1.3 | -0.5 | 5.7 | 3.9 | 2.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.8603 | 9.0435 | 8.5599 | 7.7549 | 8.4784 | 8.1531 | 3.7643 | 5.2194 | 4.9187 | 4.1509 | 4.0208 | 4.4879 | 4.3917 | 5.6084 | 5.1914 | 4.6284 | 4.4775 | 5.0062 |
Actin | 0.022 | 0.0032 | 0.0045 | 0.0376 | 0.0164 | 0.0017 | 0.0126 | 0.009 | 0.0145 | 0.001 | 0.0001 | 0.0187 | 0.0418 | 0.0005 | 0.007 | 0.01 | 0.0492 | 0.0181 |
Bud | 0.0035 | 0.0059 | 0.0073 | 0.004 | 0.0095 | 0.001 | 0.0008 | 0.0028 | 0.0038 | 0.0007 | 0.0002 | 0.0008 | 0.0018 | 0.0007 | 0.0053 | 0.0003 | 0.0168 | 0.0042 |
Bud Neck | 0.0036 | 0.0004 | 0.001 | 0.0012 | 0.002 | 0.0016 | 0.0016 | 0.0005 | 0.0048 | 0.0035 | 0.0006 | 0.002 | 0.0075 | 0.0002 | 0.0014 | 0.0016 | 0.0086 | 0.002 |
Bud Periphery | 0.0029 | 0.0052 | 0.0043 | 0.004 | 0.0067 | 0.0006 | 0.0006 | 0.0014 | 0.0058 | 0.0001 | 0 | 0.0007 | 0.0017 | 0.0003 | 0.0021 | 0.0002 | 0.012 | 0.0051 |
Bud Site | 0.0059 | 0.0106 | 0.0072 | 0.0032 | 0.0044 | 0.0003 | 0.0048 | 0.0047 | 0.0025 | 0.0018 | 0.0003 | 0.0003 | 0.0317 | 0.0035 | 0.0109 | 0.0019 | 0.0042 | 0.0008 |
Cell Periphery | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0031 | 0.0002 |
Cytoplasm | 0.8 | 0.9103 | 0.8605 | 0.7278 | 0.7395 | 0.8655 | 0.7189 | 0.9206 | 0.8252 | 0.7125 | 0.8392 | 0.8532 | 0.6062 | 0.9305 | 0.9056 | 0.9065 | 0.7686 | 0.8632 |
Cytoplasmic Foci | 0.0324 | 0.0194 | 0.0318 | 0.0556 | 0.0404 | 0.0438 | 0.0569 | 0.0202 | 0.0203 | 0.0475 | 0.0996 | 0.035 | 0.0647 | 0.0121 | 0.0084 | 0.0143 | 0.0244 | 0.0188 |
Eisosomes | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0111 | 0.0105 | 0.0063 | 0.0036 | 0.0058 | 0.007 | 0.0097 | 0.004 | 0.0067 | 0.0044 | 0.0042 | 0.0156 | 0.0117 | 0.0046 | 0.0033 | 0.0054 | 0.0094 | 0.0029 |
Endosome | 0.043 | 0.0141 | 0.0276 | 0.0957 | 0.1028 | 0.0418 | 0.0475 | 0.0073 | 0.0261 | 0.0706 | 0.0364 | 0.0351 | 0.0735 | 0.0054 | 0.0169 | 0.019 | 0.0324 | 0.0447 |
Golgi | 0.0092 | 0.0016 | 0.0053 | 0.0148 | 0.0275 | 0.0038 | 0.014 | 0.0015 | 0.0152 | 0.007 | 0.0018 | 0.0053 | 0.0213 | 0.0005 | 0.0029 | 0.0231 | 0.0236 | 0.0066 |
Lipid Particles | 0.0035 | 0.0001 | 0.0015 | 0.0083 | 0.0074 | 0.0017 | 0.0181 | 0.0002 | 0.0008 | 0.0001 | 0.0001 | 0.0041 | 0.009 | 0.0001 | 0.0033 | 0.0031 | 0.0049 | 0.0009 |
Mitochondria | 0.0045 | 0.0007 | 0.0041 | 0.0033 | 0.0131 | 0.002 | 0.0063 | 0.0008 | 0.0046 | 0.0011 | 0.0005 | 0.002 | 0.0086 | 0.0002 | 0.0078 | 0.0028 | 0.0033 | 0.0107 |
None | 0.0258 | 0.0075 | 0.0102 | 0.0034 | 0.0024 | 0.0128 | 0.0651 | 0.0203 | 0.0515 | 0.1393 | 0.0105 | 0.0128 | 0.0224 | 0.0355 | 0.0119 | 0.0048 | 0.0138 | 0.0041 |
Nuclear Periphery | 0.008 | 0.0018 | 0.0025 | 0.0023 | 0.0025 | 0.0027 | 0.0052 | 0.001 | 0.0066 | 0.0015 | 0.0009 | 0.0019 | 0.0336 | 0.0011 | 0.0016 | 0.0011 | 0.0068 | 0.002 |
Nucleolus | 0.0003 | 0.0001 | 0.004 | 0.0002 | 0.0008 | 0.0002 | 0.0009 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.002 | 0.0001 | 0.0007 | 0.0001 | 0.0006 | 0.0002 |
Nucleus | 0.0092 | 0.0034 | 0.0042 | 0.0039 | 0.004 | 0.0045 | 0.003 | 0.0022 | 0.0039 | 0.0017 | 0.0016 | 0.0024 | 0.0086 | 0.0016 | 0.0025 | 0.0018 | 0.0037 | 0.0017 |
Peroxisomes | 0.0026 | 0.0004 | 0.0073 | 0.0081 | 0.006 | 0.0017 | 0.0183 | 0.0008 | 0.0016 | 0.0016 | 0.0011 | 0.0015 | 0.0108 | 0.0003 | 0.0031 | 0.001 | 0.0035 | 0.0012 |
Punctate Nuclear | 0.0018 | 0.0007 | 0.0025 | 0.0104 | 0.0019 | 0.0019 | 0.0077 | 0.0006 | 0.0005 | 0.0005 | 0.0006 | 0.0037 | 0.0306 | 0.0004 | 0.0007 | 0.0005 | 0.0013 | 0.0012 |
Vacuole | 0.0079 | 0.0032 | 0.0066 | 0.0101 | 0.005 | 0.0041 | 0.0056 | 0.0016 | 0.0037 | 0.0039 | 0.0017 | 0.003 | 0.0068 | 0.002 | 0.003 | 0.0018 | 0.008 | 0.0072 |
Vacuole Periphery | 0.0023 | 0.0005 | 0.0013 | 0.002 | 0.0017 | 0.001 | 0.0016 | 0.0003 | 0.0014 | 0.0012 | 0.0006 | 0.0016 | 0.0048 | 0.0002 | 0.0011 | 0.0006 | 0.0019 | 0.0042 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.2056 | 16.6145 | 10.382 | 28.4299 | 19.6582 | 13.6872 | 25.4194 | 49.2313 | 50.9295 | 21.5304 |
Translational Efficiency | 0.8368 | 1.3693 | 1.1913 | 1.1667 | 1.0837 | 1.9276 | 1.2707 | 1.045 | 1.0879 | 0.9977 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
30 | 1136 | 1886 | 131 | 1467 | 959 | 292 | 1485 | 1497 | 2095 | 2178 | 1616 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2258.61 | 813.33 | 963.66 | 1963.01 | 765.15 | 866.99 | 1572.10 | 1932.48 | 795.08 | 837.89 | 1045.23 | 1934.95 |
Standard Deviation | 1143.06 | 206.32 | 235.53 | 679.97 | 128.72 | 165.51 | 428.13 | 750.03 | 293.64 | 190.62 | 339.98 | 744.64 |
Intensity Change Log 2 | — | -1.473522 | -1.228839 | -0.202368 | — | 0.180273 | 1.038878 | 1.336639 | — | -0.847608 | -0.253925 | 0.365461 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001216 | 0.001167 | 0.000298 | 0.002434 | 0.000199 | 0.000744 | 0.001935 | 0.002214 | 0.000220 | 0.000973 | 0.000517 | 0.002232 |
Bud Neck | 0.011264 | 0.013918 | 0.003966 | 0.010960 | 0.007400 | 0.009862 | 0.010066 | 0.043819 | 0.007477 | 0.012062 | 0.004784 | 0.041155 |
Bud Site | 0.043417 | 0.036778 | 0.016071 | 0.096155 | 0.011069 | 0.023658 | 0.050410 | 0.163829* | 0.011717 | 0.030772 | 0.020675 | 0.158343* |
Cell Periphery | 0.000560 | 0.000194 | 0.000167 | 0.000862 | 0.000191 | 0.000155 | 0.000263 | 0.001033 | 0.000198 | 0.000177 | 0.000180 | 0.001019 |
Cytoplasm | 0.027957 | 0.458045* | 0.706076* | 0.169454* | 0.541043* | 0.569388* | 0.451469* | 0.076198 | 0.530760* | 0.509013* | 0.671942* | 0.083757 |
Cytoplasmic Foci | 0.479284* | 0.266874* | 0.044840 | 0.544197* | 0.246211* | 0.210082* | 0.224373* | 0.407265* | 0.250882* | 0.240877* | 0.068909 | 0.418366* |
Eisosomes | 0.000128 | 0.000096 | 0.000051 | 0.000052 | 0.000111 | 0.000061 | 0.000037 | 0.000266 | 0.000111 | 0.000080 | 0.000049 | 0.000249 |
Endoplasmic Reticulum | 0.010414 | 0.001672 | 0.001443 | 0.000510 | 0.002906 | 0.002118 | 0.001139 | 0.000471 | 0.003057 | 0.001876 | 0.001402 | 0.000474 |
Endosome | 0.009938 | 0.031295 | 0.002079 | 0.005054 | 0.019951 | 0.016752 | 0.016087 | 0.015173 | 0.019750 | 0.024638 | 0.003957 | 0.014353 |
Golgi | 0.017607 | 0.013058 | 0.000283 | 0.002826 | 0.004074 | 0.006010 | 0.003149 | 0.009882 | 0.004346 | 0.009832 | 0.000667 | 0.009310 |
Lipid Particles | 0.027621 | 0.006571 | 0.000860 | 0.026649 | 0.010716 | 0.004073 | 0.005849 | 0.004018 | 0.011055 | 0.005427 | 0.001529 | 0.005853 |
Mitochondria | 0.003891 | 0.008396 | 0.000594 | 0.000249 | 0.002968 | 0.006156 | 0.000422 | 0.006472 | 0.002987 | 0.007371 | 0.000571 | 0.005967 |
Mitotic Spindle | 0.032609 | 0.018389 | 0.014426 | 0.039906 | 0.002141 | 0.018863 | 0.077876 | 0.146399* | 0.002751 | 0.018606 | 0.022933 | 0.137767* |
None | 0.003487 | 0.002901 | 0.005279 | 0.000175 | 0.003300 | 0.002419 | 0.001732 | 0.001152 | 0.003304 | 0.002680 | 0.004804 | 0.001073 |
Nuclear Periphery | 0.005781 | 0.000323 | 0.000667 | 0.003252 | 0.000517 | 0.000238 | 0.000774 | 0.000908 | 0.000623 | 0.000284 | 0.000681 | 0.001098 |
Nuclear Periphery Foci | 0.025026 | 0.001602 | 0.002546 | 0.008972 | 0.001639 | 0.001010 | 0.015548 | 0.003488 | 0.002108 | 0.001331 | 0.004289 | 0.003933 |
Nucleolus | 0.006816 | 0.001871 | 0.000866 | 0.010150 | 0.000779 | 0.001116 | 0.000964 | 0.016270 | 0.000900 | 0.001525 | 0.000879 | 0.015774 |
Nucleus | 0.097244 | 0.047406 | 0.151690* | 0.007411 | 0.077627 | 0.045679 | 0.043788 | 0.021699 | 0.078020 | 0.046615 | 0.137224* | 0.020541 |
Peroxisomes | 0.039467 | 0.010305 | 0.001065 | 0.020307 | 0.004314 | 0.006400 | 0.008582 | 0.041792 | 0.005019 | 0.008518 | 0.002073 | 0.040050 |
Vacuole | 0.054868 | 0.074365 | 0.046137 | 0.045235 | 0.060442 | 0.071722 | 0.084770 | 0.031516 | 0.060331 | 0.073155 | 0.051316 | 0.032628 |
Vacuole Periphery | 0.101405* | 0.004774 | 0.000597 | 0.005191 | 0.002402 | 0.003493 | 0.000767 | 0.006135 | 0.004386 | 0.004188 | 0.000620 | 0.006058 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.17 | 2.19 | -1.49 | -1.69 | -2.65 | -2.59 | -3.08 | -6.29 | -3.54 | -0.63 | -5.35 | -3.95 | -6.72 | -3.58 | -5.48 |
Bud Neck | -0.48 | 1.60 | 0.07 | 1.43 | -4.55 | -2.19 | -2.49 | -17.47 | -16.20 | -13.38 | -4.33 | 2.60 | -17.24 | -15.13 | -19.70 |
Bud Site | 0.83 | 2.88 | -3.32 | -4.67 | -6.23 | -4.91 | -5.65 | -27.83* | -24.39 | -15.36 | -8.33 | -3.45 | -28.21 | -23.99 | -26.54 |
Cell Periphery | 2.58 | 2.81 | -1.63 | -4.51 | -4.71 | 1.14 | -3.40 | -9.15 | -9.34 | -7.43 | 1.07 | 0.73 | -9.55 | -9.80 | -9.65 |
Cytoplasm | -23.95 | -39.41 | -6.09 | 8.99 | 17.44 | -2.34 | 4.00 | 50.03 | 43.22 | 17.56 | 2.24 | -11.50 | 47.18 | 46.71 | 67.19* |
Cytoplasmic Foci | 2.85 | 5.99 | 0.13 | -5.77 | -12.64 | 4.26 | 5.20 | -12.73 | -15.38 | -12.20 | 1.42 | 29.64 | -13.11 | -14.83 | -36.88* |
Eisosomes | 0.80 | 1.67 | 1.78 | 3.99 | -0.03 | 7.30 | 10.59 | -4.92 | -6.76 | -7.79 | 4.33 | 3.36 | -4.67 | -5.92 | -5.92 |
Endoplasmic Reticulum | 2.15 | 2.15 | 2.41 | 3.85 | 4.34 | 1.11 | 3.05 | 5.41 | 2.93 | 4.93 | 2.23 | 3.06 | 5.71 | 5.03 | 9.82 |
Endosome | -3.57 | 1.89 | 1.14 | 11.24 | -3.35 | 2.02 | 2.70 | 4.15 | 2.17 | 0.20 | -2.65 | 13.20 | 4.60 | 7.21 | -7.00 |
Golgi | 0.62 | 2.39 | 2.05 | 6.41 | -3.87 | -2.23 | 1.98 | -2.90 | -1.22 | -3.90 | -5.38 | 7.82 | -2.50 | 1.86 | -6.44 |
Lipid Particles | 0.91 | 1.16 | -0.02 | -2.55 | -3.26 | 6.46 | 3.24 | 6.34 | -0.13 | 2.64 | 5.04 | 9.76 | 3.95 | -0.54 | -4.72 |
Mitochondria | -1.85 | 1.74 | 1.91 | 5.57 | 3.09 | -2.15 | 3.98 | -3.63 | -0.01 | -9.42 | -3.66 | 3.89 | -3.20 | 1.43 | -9.29 |
Mitotic Spindle | 0.92 | 1.11 | -0.56 | -2.12 | -2.40 | -5.33 | -6.84 | -21.68 | -17.77 | -6.88 | -7.05 | -9.04 | -21.69 | -18.49 | -17.90 |
None | 0.14 | -0.44 | 1.08 | 3.43 | 8.33 | 1.12 | 2.24 | 2.95 | 2.80 | 1.35 | 0.79 | -1.10 | 3.14 | 3.13 | 6.44 |
Nuclear Periphery | 1.88 | 1.67 | 0.91 | -0.98 | -0.71 | 4.30 | -4.44 | -3.87 | -6.98 | 1.31 | 3.93 | -3.40 | -2.25 | -4.14 | -0.76 |
Nuclear Periphery Foci | 1.88 | 1.77 | 0.98 | -2.74 | -2.37 | 1.94 | -6.70 | -2.54 | -3.93 | 5.76 | 1.85 | -4.78 | -2.64 | -4.27 | 0.88 |
Nucleolus | 1.45 | 1.78 | -0.12 | -0.98 | -1.17 | -1.20 | -0.55 | -10.51 | -9.61 | -10.16 | -2.49 | 0.16 | -10.16 | -9.43 | -10.11 |
Nucleus | 0.91 | -1.48 | 1.64 | 9.06 | 30.75 | 8.63 | 2.39 | 13.57 | 4.79 | 4.77 | 8.87 | -15.83 | 14.07 | 6.72 | 29.22 |
Peroxisomes | 1.78 | 2.31 | 1.37 | -1.38 | -3.49 | -1.71 | -0.67 | -13.49 | -12.51 | -12.40 | -2.91 | 3.69 | -13.03 | -11.94 | -16.35 |
Vacuole | -0.92 | -0.66 | -0.55 | 0.51 | 0.05 | -2.82 | -6.80 | 6.17 | 7.85 | 8.76 | -4.08 | -5.16 | 5.50 | 9.51 | 10.17 |
Vacuole Periphery | 2.42 | 2.53 | 2.44 | 0.31 | -1.02 | -1.17 | 4.56 | -3.54 | -1.63 | -6.14 | 0.16 | 4.21 | -0.74 | -1.16 | -6.36 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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