Standard name
Human Ortholog
Description Translation termination factor eRF3; has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that modifies cellular fitness, alters translational fidelity by affecting reading frame selection, and results in a nonsense suppressor phenotype; many stress-response genes are repressed in the presence of [PSI(+)]
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0.09 0.05 0 0.07 0.11 0.08 0.06 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.97 0.96 0.96 0.87 0.91 0.86 0.87 0.84 0.73 0.88 0.96 0.96 0.99 – – – 0.98 0.94 0.92 0.91 0.94 0.88
Endoplasmic Reticulum 0 0 0 0 0.05 0.09 0.08 0.09 0.07 0.09 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 1 1 6 7 2 0 0 0 – – – 0 0 0 0 0 1
Bud 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 – – – 0 2 1 2 2 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 2 4 2 0 9 7 5 4 14 8 5 3 5 5 – – – 0 0 0 0 0 0
Cytoplasm 62 206 131 54 76 87 141 117 47 108 69 72 135 143 144 – – – 83 197 123 110 148 147
Endoplasmic Reticulum 0 2 3 1 4 9 13 12 4 12 4 2 4 5 1 – – – 0 2 3 2 1 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 1 1 0 0 1 0 0 0 4 2 1 0 1 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 1 0 1 6 2 0 0 0 – – – 0 0 0 0 0 2
Nucleus 0 0 0 0 0 0 0 0 0 0 2 1 1 0 0 – – – 0 1 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 1 0 0 0 1 0 0 6 0 1 1 1 – – – 0 2 2 2 1 1
Unique Cell Count 62 208 135 56 79 100 155 136 54 129 95 82 140 149 146 85 211 134 121 158 168
Labelled Cell Count 62 212 139 58 80 107 162 137 58 145 106 85 144 155 151 85 211 134 121 158 168


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 52.3 77.4 56.7 50.1 46.2 41.7 40.4 37.8 36.0 36.0 31.0 31.7 54.2 53.1 52.5 – – – 47.2 51.6 50.7
Std Deviation (1e-4) 11.0 15.8 11.0 9.7 11.3 11.0 9.3 10.4 10.2 9.0 9.8 8.7 17.5 18.8 20.8 – – – 15.9 15.6 20.8
Intensity Change (Log2) – – – -0.18 -0.3 -0.44 -0.49 -0.58 -0.66 -0.65 -0.87 -0.84 -0.06 -0.1 -0.11 – – – -0.26 -0.14 -0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 2.0 0.7 0 0 2.5 0 0 0 0 0 – – –
Cytoplasm -0.2 -0.3 -2.9 -2.1 -3.3 -2.7 -3.7 -5.4 -2.7 -0.3 -0.5 0.9 – – –
Endoplasmic Reticulum 0 0 2.3 2.3 2.4 0 2.5 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 32.7692 56.266 49.9468 44.9428 45.1867 52.0806 40.6717 55.253 56.2708 46.0926 30.9552 53.7072 44.386 66.7109 62.1454 53.8799 41.6566 62.2976
Actin 0.0099 0.0018 0.0195 0.044 0.0021 0.0011 0.0166 0.0023 0.0146 0.0131 0.0015 0.0022 0.0106 0.0004 0.0028 0.0416 0.078 0.0023
Bud 0.0017 0.0005 0.0029 0.0037 0.0066 0.0025 0.0035 0.0008 0.0022 0.0076 0.0024 0.002 0.005 0.0008 0.0018 0.0027 0.0052 0.0015
Bud Neck 0.0012 0.0016 0.0014 0.0009 0.0011 0.0161 0.0023 0.0007 0.0017 0.0032 0.0011 0.005 0.0035 0.001 0.0043 0.0034 0.0025 0.0046
Bud Periphery 0.0018 0.0003 0.0043 0.0074 0.0235 0.0024 0.0068 0.0006 0.0016 0.0263 0.0008 0.0013 0.0116 0.0006 0.0025 0.0045 0.0042 0.0015
Bud Site 0.0017 0.0022 0.0059 0.004 0.0019 0.0004 0.0028 0.0021 0.0046 0.0012 0.0058 0.0004 0.0056 0.001 0.0022 0.0049 0.003 0.0003
Cell Periphery 0.0061 0.0248 0.0145 0.0707 0.0014 0.0043 0.0134 0.0158 0.016 0.0122 0.0036 0.0035 0.0516 0.0144 0.0187 0.0223 0.0041 0.0075
Cytoplasm 0.6326 0.8885 0.7853 0.3927 0.8378 0.8565 0.6738 0.874 0.8288 0.5093 0.6094 0.8764 0.5785 0.8925 0.799 0.5893 0.6539 0.8799
Cytoplasmic Foci 0.0107 0.0006 0.0024 0.006 0.0014 0.0034 0.0031 0.0009 0.0025 0.0034 0.1178 0.0036 0.0109 0.0009 0.0012 0.0063 0.0077 0.0031
Eisosomes 0.0002 0 0.0001 0.0009 0 0 0.0001 0.0006 0 0.0001 0.0004 0 0.0004 0 0.0001 0.001 0.0001 0
Endoplasmic Reticulum 0.0518 0.0081 0.0062 0.0066 0.0022 0.0251 0.0563 0.003 0.0108 0.0065 0.0044 0.0304 0.05 0.0081 0.0209 0.0044 0.0106 0.0351
Endosome 0.0378 0.0008 0.0104 0.003 0.0081 0.0074 0.0217 0.0017 0.005 0.0664 0.023 0.017 0.0435 0.0015 0.0034 0.0178 0.0441 0.0055
Golgi 0.0051 0.0002 0.0024 0.0015 0.0014 0.0008 0.0024 0.0002 0.0009 0.0031 0.0018 0.0033 0.0146 0.0001 0.0003 0.0038 0.0266 0.0017
Lipid Particles 0.0065 0.0011 0.0006 0.0051 0.0004 0.0003 0.0014 0.0017 0.0013 0.0013 0.0162 0.0008 0.0078 0.0014 0.0009 0.0049 0.0018 0.0012
Mitochondria 0.0124 0.0001 0.0062 0.0561 0.061 0.0005 0.0087 0.0154 0.0005 0.0145 0.0065 0.001 0.0067 0.0001 0.0002 0.0087 0.008 0.0012
None 0.003 0.0001 0.0007 0.0218 0.0003 0.0002 0.0024 0.0007 0.0002 0.0004 0.0465 0.0005 0.0026 0.0003 0.0002 0.0084 0.0012 0.0004
Nuclear Periphery 0.0754 0.0033 0.0109 0.0092 0.0031 0.0049 0.0294 0.0021 0.0051 0.0064 0.0072 0.007 0.0365 0.0028 0.0049 0.0102 0.0196 0.0061
Nucleolus 0.0016 0.0002 0.0004 0.0022 0.0005 0.0001 0.0007 0.0006 0.0002 0.0005 0.0142 0.0003 0.0013 0.0002 0.0002 0.0115 0.0003 0.0004
Nucleus 0.0247 0.0021 0.0129 0.0053 0.0038 0.0034 0.0132 0.0023 0.0032 0.0046 0.011 0.004 0.0121 0.0021 0.0026 0.01 0.0485 0.0063
Peroxisomes 0.0005 0 0.0002 0.0013 0.0003 0.0001 0.0002 0.0001 0.0002 0.0001 0.0017 0.0001 0.0009 0 0 0.0011 0.0032 0.0001
Punctate Nuclear 0.0011 0 0.0008 0.0008 0.0001 0.0001 0.0009 0.0001 0.0001 0.0001 0.0125 0.0002 0.001 0 0.0001 0.0014 0.0008 0.0002
Vacuole 0.0946 0.0618 0.0958 0.3278 0.0326 0.0628 0.126 0.0673 0.0944 0.2876 0.0895 0.0327 0.1346 0.0687 0.1231 0.2059 0.0652 0.0358
Vacuole Periphery 0.0198 0.0019 0.0162 0.0291 0.0102 0.0076 0.0144 0.0069 0.0061 0.0323 0.0228 0.0083 0.0106 0.0031 0.0108 0.036 0.0113 0.0053

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 190.6027 172.5439 144.3951 163.629 181.4333 142.0021 141.8691 148.984 140.3417 158.7992
Translational Efficiency 2.2733 2.4678 2.4435 1.9632 1.9055 2.3061 2.129 1.8635 1.8122 1.8628

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
30 1407 2231 294 154 2147 194 14 184 3554 2425 308

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1197.17 1339.63 1634.80 2130.64 1392.62 1378.36 1999.67 322.09 1360.75 1363.03 1663.99 2048.43
Standard Deviation 237.18 369.11 342.16 547.89 252.93 322.21 293.18 14.85 260.63 342.08 352.68 654.57
Intensity Change Log 2 — 0.162207 0.449486 0.831659 — -0.014849 0.521960 -2.112266 — 0.069705 0.488910 -0.078447

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000787 0.000565 0.000675 0.006015 0.000665 0.000705 0.001191 0.000093 0.000685 0.000649 0.000716 0.005745
Bud Neck 0.007707 0.002043 0.008589 0.017995 0.004491 0.004097 0.012774 0.000005 0.005015 0.003284 0.008924 0.017178
Bud Site 0.000928 0.000330 0.000696 0.006723 0.000488 0.000739 0.001429 0.000016 0.000559 0.000578 0.000754 0.006418
Cell Periphery 0.000127 0.000098 0.000022 0.000265 0.000034 0.000102 0.000042 0.000261 0.000049 0.000101 0.000023 0.000265
Cytoplasm 0.000470 0.000420 0.000030 0.001152 0.000040 0.000245 0.000095 0.000040 0.000111 0.000314 0.000035 0.001102
Cytoplasmic Foci 0.000082 0.000227 0.000120 0.006717 0.000122 0.000371 0.000412 0.000019 0.000115 0.000314 0.000143 0.006413
Eisosomes 0.000068 0.000131 0.000143 0.000148 0.000085 0.000163 0.000190 0.001057 0.000082 0.000150 0.000147 0.000190
Endoplasmic Reticulum 0.001301 0.000434 0.000240 0.001445 0.000428 0.000641 0.000445 0.000001 0.000570 0.000559 0.000256 0.001379
Endosome 0.000124 0.000149 0.000007 0.004328 0.000030 0.000207 0.000013 0.000000 0.000045 0.000184 0.000008 0.004132
Golgi 0.000097 0.000174 0.000052 0.002477 0.000081 0.000198 0.000122 0.000004 0.000084 0.000189 0.000058 0.002364
Lipid Particles 0.000010 0.000626 0.000003 0.000848 0.000007 0.000341 0.000013 0.000012 0.000008 0.000454 0.000004 0.000810
Mitochondria 0.001298 0.001048 0.001543 0.005221 0.001452 0.001297 0.003091 0.000012 0.001427 0.001198 0.001667 0.004984
Mitotic Spindle 0.003113 0.000739 0.000284 0.023272 0.003897 0.002942 0.000515 0.000171 0.003769 0.002070 0.000302 0.022222
None 0.002552 0.001706 0.000524 0.002787 0.000798 0.001346 0.000990 0.998090* 0.001084 0.001488 0.000562 0.048028
Nuclear Periphery 0.000117 0.000064 0.000045 0.000362 0.000125 0.000139 0.000071 0.000000 0.000123 0.000109 0.000047 0.000346
Nuclear Periphery Foci 0.000023 0.000193 0.000009 0.000252 0.000031 0.000616 0.000006 0.000001 0.000030 0.000448 0.000009 0.000241
Nucleolus 0.114700 0.096787 0.083923 0.134133 0.161328 0.113913 0.098246 0.000002 0.153726 0.107133 0.085069 0.128036
Nucleus 0.862282* 0.887722* 0.897554* 0.757328* 0.815913* 0.868020* 0.861365* 0.000006 0.823473* 0.875820* 0.894659* 0.722904*
Peroxisomes 0.003484 0.004908 0.005193 0.018960 0.009593 0.003082 0.018343 0.000207 0.008597 0.003805 0.006245 0.018108
Vacuole 0.000450 0.000890 0.000113 0.004120 0.000134 0.000394 0.000381 0.000000 0.000186 0.000590 0.000134 0.003933
Vacuole Periphery 0.000280 0.000746 0.000234 0.005451 0.000259 0.000445 0.000263 0.000000 0.000263 0.000564 0.000237 0.005203

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.97 0.88 -1.70 -1.82 -1.75 NaN NaN NaN NaN NaN 0.63 -0.92 -1.74 -1.76 -1.72
Bud Neck 3.16 -0.40 -4.08 -6.99 -4.62 NaN NaN NaN NaN NaN 3.19 -6.49 -5.65 -6.47 -4.30
Bud Site 1.18 0.80 -3.12 -4.85 -4.56 NaN NaN NaN NaN NaN 0.48 -0.69 -4.52 -4.63 -4.47
Cell Periphery 0.68 1.75 -1.02 -2.00 -3.24 NaN NaN NaN NaN NaN -1.84 1.47 -2.85 -2.18 -3.36
Cytoplasm 0.66 1.38 -0.67 -1.76 -2.80 NaN NaN NaN NaN NaN -1.16 1.38 -2.43 -2.08 -2.79
Cytoplasmic Foci -0.45 0.23 -2.98 -2.95 -2.99 NaN NaN NaN NaN NaN -2.36 -0.63 -2.99 -2.89 -2.97
Eisosomes -3.87 -5.98 -3.59 -1.20 -0.60 NaN NaN NaN NaN NaN -6.26 -7.20 -3.48 -1.50 -1.49
Endoplasmic Reticulum 1.99 2.41 0.12 -1.81 -2.23 NaN NaN NaN NaN NaN -0.03 3.11 -1.41 -1.42 -2.15
Endosome -0.15 1.72 -2.10 -2.09 -2.20 NaN NaN NaN NaN NaN -2.87 2.31 -2.17 -2.05 -2.20
Golgi -0.54 1.70 -2.46 -2.37 -2.55 NaN NaN NaN NaN NaN -1.85 1.82 -2.50 -2.32 -2.53
Lipid Particles -1.11 2.17 -1.45 0.07 -1.47 NaN NaN NaN NaN NaN -1.90 2.17 -1.46 -0.16 -1.47
Mitochondria 1.29 -0.97 -5.43 -6.35 -5.44 NaN NaN NaN NaN NaN 1.63 -1.71 -5.38 -6.00 -5.08
Mitotic Spindle 0.93 0.96 -1.60 -3.70 -3.75 NaN NaN NaN NaN NaN 1.01 1.68 -2.90 -3.48 -3.73
None 1.04 1.70 0.51 -1.29 -3.26 NaN NaN NaN NaN NaN -0.57 2.11 -3.93 -3.91 -3.99
Nuclear Periphery 1.21 1.38 -2.39 -5.61 -6.18 NaN NaN NaN NaN NaN 0.59 2.17 -3.94 -4.74 -6.08
Nuclear Periphery Foci -1.78 1.29 -3.39 -0.72 -3.93 NaN NaN NaN NaN NaN -3.17 2.20 -3.52 1.49 -3.92
Nucleolus 0.63 0.89 -1.23 -4.94 -5.68 NaN NaN NaN NaN NaN 3.39 4.54 0.24 -3.72 -5.09
Nucleus -1.08 -1.17 3.32 9.04 9.32 NaN NaN NaN NaN NaN -3.49 -4.21 5.24 9.63 10.22
Peroxisomes -1.16 -2.30 -5.94 -5.65 -5.42 NaN NaN NaN NaN NaN 2.73 0.99 -3.76 -6.01 -4.94
Vacuole -0.95 1.07 -2.14 -1.84 -2.41 NaN NaN NaN NaN NaN -2.70 0.70 -2.36 -2.05 -2.38
Vacuole Periphery -1.34 0.87 -1.68 -1.45 -1.71 NaN NaN NaN NaN NaN -2.21 0.96 -1.69 -1.54 -1.70
Standard name
Human Ortholog
Description Translation termination factor eRF3; has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that modifies cellular fitness, alters translational fidelity by affecting reading frame selection, and results in a nonsense suppressor phenotype; many stress-response genes are repressed in the presence of [PSI(+)]
Localization
Cell Percentages nucleus (88%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Sup35

Sup35


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sup35-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available