Standard name | |
---|---|
Human Ortholog | |
Description | Protein involved in SRP-independent targeting of substrates to the ER; component of an alternative ER targeting pathway that has partial functional redundancy with the GET pathway; preference for substrates with downstream transmembrane domains; interacts with Env10p/Snd2p and Pho88p/Snd3p; can compensate for loss of SRP; may interact with ribosomes, based on co-purification experiments; GFP-fusion protein localizes to the cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 4 | 1 |
Bud | 0 | 0 | 3 | 0 | 0 | 0 | 1 | 3 | 5 | – | 4 | 6 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 6 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 4 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 5 | 9 | 5 | 9 | 4 | 4 | 8 | 0 | – | 2 | 9 | 2 | 14 | 5 | 3 | 6 | 7 | 0 | 0 | 0 | 0 | 1 | 1 |
Cytoplasm | 145 | 172 | 276 | 167 | 293 | 209 | 303 | 274 | 160 | – | 118 | 208 | 228 | 420 | 416 | 162 | 157 | 156 | 139 | 176 | 258 | 122 | 378 | 351 |
Endoplasmic Reticulum | 2 | 7 | 5 | 1 | 1 | 0 | 2 | 0 | 1 | – | 1 | 2 | 0 | 0 | 1 | 10 | 5 | 12 | 4 | 1 | 5 | 11 | 38 | 25 |
Endosome | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | – | 1 | 0 | 0 | 1 | 2 | 2 | 2 | 1 | 3 | 1 | 0 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 1 |
Mitochondria | 10 | 1 | 0 | 9 | 6 | 39 | 43 | 26 | 60 | – | 82 | 77 | 10 | 4 | 6 | 5 | 3 | 8 | 2 | 2 | 12 | 11 | 17 | 11 |
Nucleus | 2 | 0 | 3 | 1 | 1 | 1 | 3 | 6 | 0 | – | 7 | 3 | 0 | 0 | 1 | 4 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 3 | 0 | 3 | 2 | 3 | 0 | 3 | 4 | 0 | – | 1 | 0 | 0 | 1 | 2 | 14 | 15 | 23 | 2 | 3 | 5 | 8 | 25 | 23 |
Unique Cell Count | 156 | 178 | 287 | 170 | 301 | 225 | 331 | 299 | 189 | 172 | 264 | 230 | 422 | 422 | 181 | 177 | 182 | 161 | 189 | 294 | 163 | 486 | 430 | |
Labelled Cell Count | 166 | 186 | 301 | 185 | 314 | 254 | 360 | 326 | 228 | 216 | 310 | 242 | 443 | 433 | 202 | 192 | 211 | 161 | 189 | 294 | 163 | 486 | 430 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.5 | 7.6 | 6.9 | 6.0 | 6.7 | 4.8 | 5.2 | 5.5 | 4.4 | – | 4.1 | 4.7 | 6.8 | 6.8 | 6.8 | 9.4 | 9.4 | 9.5 | 6.6 | 6.6 | 7.0 |
Std Deviation (1e-4) | 0.9 | 1.1 | 1.2 | 0.9 | 1.7 | 1.1 | 1.4 | 1.5 | 1.3 | – | 1.1 | 1.2 | 1.2 | 1.0 | 1.4 | 1.4 | 1.6 | 1.5 | 1.5 | 1.3 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.21 | -0.05 | -0.52 | -0.42 | -0.33 | -0.65 | – | -0.77 | -0.55 | -0.02 | -0.03 | -0.02 | 0.44 | 0.44 | 0.47 | -0.07 | -0.06 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.1 | -0.1 | 0 | -1.7 | -0.3 | 0 | – | 0 | 0 | 0 | 0.1 | -1.8 | 0 | 0.2 | 0.4 |
Cytoplasm | 1.2 | 0.8 | -1.6 | -2.4 | -2.3 | -4.4 | – | -8.2 | -6.2 | 2.1 | 3.3 | 2.1 | -2.9 | -3.1 | -4.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 | 2.7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 6.3 | 5.1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.8 | 4.0 | 5.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.1812 | 4.4647 | 3.5351 | 3.4841 | 2.0809 | 3.6031 | 2.4584 | 3.0657 | 2.9142 | 2.3862 | 2.6228 | 2.8981 | 2.1851 | 2.8923 | 2.6754 | 2.8573 | 1.8461 | 2.5189 |
Actin | 0.0137 | 0.0003 | 0.0138 | 0.0097 | 0.0002 | 0.0036 | 0.0205 | 0.001 | 0.0241 | 0.0174 | 0.0004 | 0.0033 | 0.0466 | 0.011 | 0.0164 | 0.0311 | 0.0054 | 0.0066 |
Bud | 0.0001 | 0.0006 | 0.0014 | 0.0009 | 0.0005 | 0.0005 | 0.0015 | 0.0025 | 0.0168 | 0.0019 | 0.0006 | 0.0018 | 0.0053 | 0.0139 | 0.0123 | 0.0153 | 0.0075 | 0.0077 |
Bud Neck | 0.0003 | 0.0001 | 0.0032 | 0.0018 | 0.0004 | 0.0014 | 0.0014 | 0.0007 | 0.0018 | 0.0025 | 0.0083 | 0.0018 | 0.0027 | 0.0005 | 0.0031 | 0.0019 | 0.0012 | 0.0015 |
Bud Periphery | 0.0002 | 0.0009 | 0.0014 | 0.0008 | 0.0005 | 0.0008 | 0.0015 | 0.0016 | 0.0134 | 0.0021 | 0.0003 | 0.0018 | 0.007 | 0.0092 | 0.0123 | 0.0147 | 0.0047 | 0.0122 |
Bud Site | 0.0009 | 0.0018 | 0.0177 | 0.0035 | 0.0003 | 0.0003 | 0.0115 | 0.0123 | 0.0135 | 0.0056 | 0.001 | 0.0009 | 0.0077 | 0.0117 | 0.0134 | 0.0035 | 0.0058 | 0.0012 |
Cell Periphery | 0.0008 | 0.0007 | 0.0009 | 0.0004 | 0.0014 | 0.0003 | 0.0004 | 0.0004 | 0.0021 | 0.0029 | 0.0001 | 0.0002 | 0.0007 | 0.0008 | 0.0005 | 0.0006 | 0.0002 | 0.0004 |
Cytoplasm | 0.5457 | 0.7225 | 0.6179 | 0.793 | 0.5957 | 0.73 | 0.6756 | 0.8789 | 0.8024 | 0.711 | 0.8531 | 0.8625 | 0.661 | 0.8394 | 0.6954 | 0.5671 | 0.7451 | 0.7937 |
Cytoplasmic Foci | 0.0164 | 0.014 | 0.019 | 0.0621 | 0.0189 | 0.0298 | 0.0258 | 0.0259 | 0.0312 | 0.0526 | 0.0407 | 0.0435 | 0.04 | 0.0281 | 0.0293 | 0.0529 | 0.0447 | 0.0421 |
Eisosomes | 0.0007 | 0.0001 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0147 | 0.0066 | 0.0062 | 0.0043 | 0.004 | 0.0176 | 0.0128 | 0.0038 | 0.0049 | 0.0037 | 0.0028 | 0.0053 | 0.0099 | 0.0025 | 0.0027 | 0.0033 | 0.0026 | 0.0027 |
Endosome | 0.037 | 0.0133 | 0.0216 | 0.023 | 0.0081 | 0.032 | 0.082 | 0.019 | 0.0293 | 0.0841 | 0.0697 | 0.0345 | 0.0325 | 0.0158 | 0.0227 | 0.0458 | 0.0403 | 0.0305 |
Golgi | 0.0042 | 0.001 | 0.0039 | 0.0016 | 0.0005 | 0.0105 | 0.015 | 0.0027 | 0.0064 | 0.0196 | 0.009 | 0.006 | 0.0081 | 0.0016 | 0.004 | 0.0158 | 0.0142 | 0.0024 |
Lipid Particles | 0.016 | 0.0005 | 0.002 | 0.0007 | 0.0057 | 0.0019 | 0.0101 | 0.0003 | 0.002 | 0.0122 | 0.0011 | 0.0004 | 0.0107 | 0.0002 | 0.002 | 0.0112 | 0.0216 | 0.0008 |
Mitochondria | 0.0028 | 0.0008 | 0.0026 | 0.0041 | 0.0338 | 0.0349 | 0.0187 | 0.0015 | 0.0066 | 0.0497 | 0.0008 | 0.0101 | 0.0049 | 0.0014 | 0.009 | 0.0047 | 0.0259 | 0.0034 |
None | 0.2659 | 0.2266 | 0.258 | 0.0232 | 0.2429 | 0.1269 | 0.0659 | 0.0402 | 0.0269 | 0.0059 | 0.0044 | 0.0154 | 0.1263 | 0.0565 | 0.0659 | 0.1623 | 0.0612 | 0.0702 |
Nuclear Periphery | 0.0348 | 0.001 | 0.0026 | 0.0053 | 0.001 | 0.0015 | 0.0192 | 0.0007 | 0.0021 | 0.0009 | 0.0004 | 0.0025 | 0.0041 | 0.0009 | 0.0053 | 0.0025 | 0.0016 | 0.0028 |
Nucleolus | 0.002 | 0.0001 | 0.0011 | 0.0074 | 0.0604 | 0.0001 | 0.0031 | 0.0001 | 0.0003 | 0.0006 | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0.0012 | 0.0007 | 0.0003 | 0.0039 |
Nucleus | 0.0223 | 0.001 | 0.0029 | 0.0391 | 0.0092 | 0.0009 | 0.0079 | 0.0016 | 0.0017 | 0.0012 | 0.0007 | 0.0033 | 0.0076 | 0.0015 | 0.0917 | 0.004 | 0.0019 | 0.0109 |
Peroxisomes | 0.0046 | 0.0001 | 0.0024 | 0.0017 | 0.0017 | 0.0006 | 0.0035 | 0.0004 | 0.0032 | 0.0106 | 0.001 | 0.0015 | 0.0094 | 0.0005 | 0.003 | 0.0497 | 0.007 | 0.0013 |
Punctate Nuclear | 0.0115 | 0.0003 | 0.0129 | 0.0025 | 0.0005 | 0.0008 | 0.0054 | 0.0008 | 0.003 | 0.0005 | 0.0004 | 0.0006 | 0.0058 | 0.0004 | 0.0044 | 0.0048 | 0.0019 | 0.0015 |
Vacuole | 0.0038 | 0.0064 | 0.0071 | 0.0124 | 0.0068 | 0.004 | 0.0136 | 0.005 | 0.0069 | 0.012 | 0.0041 | 0.0034 | 0.0073 | 0.0036 | 0.0044 | 0.0066 | 0.0053 | 0.0032 |
Vacuole Periphery | 0.0015 | 0.0008 | 0.0009 | 0.0024 | 0.0069 | 0.0014 | 0.0043 | 0.0006 | 0.0011 | 0.0026 | 0.0009 | 0.001 | 0.0015 | 0.0005 | 0.001 | 0.0015 | 0.0015 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 37.4923 | 25.4645 | 25.5649 | 34.3986 | 38.834 | 39.3556 | 37.0115 | 36.6718 | 35.4835 | 42.7385 |
Translational Efficiency | 0.7572 | 0.7703 | 0.7671 | 0.5038 | 0.5074 | 0.7792 | 0.6066 | 0.6194 | 0.6425 | 0.6265 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2294 | 1415 | 295 | 1822 | 1489 | 1393 | 134 | 67 | 3783 | 2808 | 429 | 1889 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 827.83 | 858.75 | 1240.88 | 1002.64 | 808.14 | 812.15 | 1059.63 | 976.83 | 820.08 | 835.63 | 1184.27 | 1001.72 |
Standard Deviation | 97.52 | 126.13 | 162.62 | 131.86 | 103.39 | 113.37 | 123.86 | 134.58 | 100.34 | 122.21 | 173.30 | 132.04 |
Intensity Change Log 2 | — | 0.052904 | 0.583957 | 0.276397 | — | 0.007141 | 0.390883 | 0.273502 | — | 0.030479 | 0.491807 | 0.274968 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000081 | 0.000212 | 0.000838 | 0.000545 | 0.000159 | 0.000400 | 0.000208 | 0.000243 | 0.000112 | 0.000305 | 0.000641 | 0.000535 |
Bud Neck | 0.004795 | 0.005766 | 0.003004 | 0.003051 | 0.004572 | 0.009770 | 0.010275 | 0.004264 | 0.004708 | 0.007752 | 0.005275 | 0.003094 |
Bud Site | 0.011982 | 0.018503 | 0.011186 | 0.030973 | 0.018553 | 0.022234 | 0.010686 | 0.044703 | 0.014568 | 0.020354 | 0.011030 | 0.031460 |
Cell Periphery | 0.000132 | 0.000076 | 0.000219 | 0.000050 | 0.000123 | 0.000134 | 0.000107 | 0.000152 | 0.000128 | 0.000105 | 0.000184 | 0.000054 |
Cytoplasm | 0.689227* | 0.673723* | 0.633192* | 0.790863* | 0.670620* | 0.554416* | 0.513722* | 0.669796* | 0.681903* | 0.614537* | 0.595875* | 0.786569* |
Cytoplasmic Foci | 0.148207 | 0.168081 | 0.044261 | 0.029328 | 0.201860* | 0.215322* | 0.048890 | 0.118375 | 0.169325 | 0.191517* | 0.045707 | 0.032486 |
Eisosomes | 0.000041 | 0.000029 | 0.000025 | 0.000009 | 0.000041 | 0.000073 | 0.000053 | 0.000023 | 0.000041 | 0.000051 | 0.000034 | 0.000009 |
Endoplasmic Reticulum | 0.001759 | 0.000530 | 0.002926 | 0.000216 | 0.001298 | 0.000534 | 0.001502 | 0.000283 | 0.001577 | 0.000532 | 0.002482 | 0.000218 |
Endosome | 0.011213 | 0.015351 | 0.005379 | 0.003348 | 0.013017 | 0.027147 | 0.009999 | 0.006818 | 0.011923 | 0.021203 | 0.006822 | 0.003471 |
Golgi | 0.007440 | 0.005066 | 0.000477 | 0.002154 | 0.008068 | 0.008184 | 0.000153 | 0.000516 | 0.007687 | 0.006612 | 0.000375 | 0.002096 |
Lipid Particles | 0.001856 | 0.001163 | 0.002331 | 0.000272 | 0.001579 | 0.003319 | 0.002061 | 0.000546 | 0.001747 | 0.002233 | 0.002247 | 0.000282 |
Mitochondria | 0.002232 | 0.003378 | 0.000228 | 0.001968 | 0.002512 | 0.004286 | 0.000061 | 0.000061 | 0.002342 | 0.003828 | 0.000176 | 0.001900 |
Mitotic Spindle | 0.000784 | 0.006684 | 0.019901 | 0.024307 | 0.001323 | 0.003867 | 0.003676 | 0.004223 | 0.000996 | 0.005286 | 0.014833 | 0.023595 |
None | 0.003504 | 0.000826 | 0.004581 | 0.001761 | 0.001869 | 0.000789 | 0.000795 | 0.001938 | 0.002861 | 0.000807 | 0.003398 | 0.001767 |
Nuclear Periphery | 0.000070 | 0.000107 | 0.000894 | 0.000121 | 0.000046 | 0.000152 | 0.000772 | 0.000100 | 0.000060 | 0.000129 | 0.000856 | 0.000120 |
Nuclear Periphery Foci | 0.000215 | 0.000279 | 0.006979 | 0.000685 | 0.000284 | 0.000322 | 0.004594 | 0.002840 | 0.000242 | 0.000300 | 0.006234 | 0.000761 |
Nucleolus | 0.000372 | 0.000377 | 0.000152 | 0.000126 | 0.000216 | 0.000368 | 0.000178 | 0.000083 | 0.000310 | 0.000373 | 0.000160 | 0.000125 |
Nucleus | 0.019561 | 0.019424 | 0.135138* | 0.030473 | 0.015237 | 0.026184 | 0.057850 | 0.012812 | 0.017859 | 0.022778 | 0.110997 | 0.029847 |
Peroxisomes | 0.000932 | 0.002280 | 0.000360 | 0.000784 | 0.001254 | 0.004616 | 0.000314 | 0.001538 | 0.001059 | 0.003439 | 0.000346 | 0.000811 |
Vacuole | 0.091034 | 0.074863 | 0.127659 | 0.077189 | 0.053327 | 0.114867 | 0.333821* | 0.130377 | 0.076192 | 0.094708 | 0.192054 | 0.079075 |
Vacuole Periphery | 0.004564 | 0.003281 | 0.000268 | 0.001778 | 0.004042 | 0.003017 | 0.000282 | 0.000307 | 0.004359 | 0.003150 | 0.000272 | 0.001726 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.25 | -8.87 | -3.70 | -1.96 | 5.11 | -3.08 | -3.44 | -1.89 | 0.55 | -0.41 | -4.77 | -8.99 | -3.40 | -0.80 | 4.14 |
Bud Neck | -1.46 | 0.74 | 5.72 | 4.96 | 4.09 | -6.42 | -0.26 | 0.99 | 4.06 | 0.60 | -5.90 | -0.09 | 5.80 | 9.10 | 1.95 |
Bud Site | -4.41 | -0.82 | -3.67 | -0.11 | -2.93 | -2.02 | 3.62 | -1.50 | -1.18 | -2.28 | -5.08 | 2.49 | -2.62 | 0.52 | -3.84 |
Cell Periphery | 6.31 | -5.96 | 13.57 | 4.76 | 9.90 | -0.74 | 0.14 | -0.28 | 0.12 | -0.33 | 2.35 | -5.63 | 14.60 | 6.82 | 10.60 |
Cytoplasm | 2.25 | 10.27 | -4.13 | -5.57 | -11.96 | 12.12 | 9.03 | 2.61 | -0.57 | -2.92 | 10.88 | 13.39 | -4.80 | -13.32 | -15.09 |
Cytoplasmic Foci | -3.51 | 24.66 | 35.13 | 29.26 | 2.95 | -1.93 | 27.49 | 6.82 | 7.62 | -3.72 | -5.01 | 36.02 | 45.74 | 43.03 | 2.71 |
Eisosomes | 3.61 | 1.95 | 12.56 | 8.03 | 8.37 | -7.21 | -2.98 | 2.90 | 8.03 | 4.57 | -3.45 | -0.09 | 17.25 | 17.27 | 11.73 |
Endoplasmic Reticulum | 4.97 | -2.42 | 6.65 | 4.28 | 5.11 | 3.21 | -1.76 | 3.98 | 2.47 | 6.79 | 5.95 | -2.83 | 8.13 | 6.78 | 6.22 |
Endosome | -3.44 | 6.54 | 15.45 | 12.33 | 6.31 | -8.46 | 4.76 | 5.28 | 11.90 | 1.30 | -9.17 | 8.18 | 18.13 | 20.57 | 7.91 |
Golgi | 3.90 | 23.15 | 14.27 | 6.05 | -1.52 | -0.04 | 21.09 | 19.66 | 9.56 | -2.28 | 2.17 | 31.07 | 16.68 | 9.44 | -1.81 |
Lipid Particles | 3.03 | -4.86 | 10.37 | 3.74 | 10.24 | -2.47 | -1.31 | 4.63 | 3.82 | 4.67 | -1.32 | -5.04 | 12.29 | 4.98 | 11.55 |
Mitochondria | -1.36 | 5.59 | 2.46 | 2.91 | -1.53 | -1.60 | 4.10 | 4.11 | 4.63 | 0.22 | -2.19 | 6.72 | 2.89 | 4.15 | -1.69 |
Mitotic Spindle | -3.67 | -3.67 | -6.62 | -3.06 | 0.91 | -2.23 | -1.09 | -1.74 | -0.85 | 0.12 | -4.36 | -3.73 | -6.61 | -4.01 | 0.37 |
None | 5.20 | 0.36 | 3.73 | -5.02 | 2.76 | 1.79 | 1.56 | 0.47 | -0.67 | -0.53 | 5.23 | 0.62 | 3.31 | -5.06 | 2.21 |
Nuclear Periphery | -1.77 | -12.58 | -11.03 | -4.56 | 11.09 | -2.59 | -7.06 | -3.61 | -1.32 | 5.27 | -3.02 | -14.37 | -12.76 | -3.75 | 12.34 |
Nuclear Periphery Foci | -1.89 | -12.30 | -9.32 | -7.99 | 11.41 | -0.79 | -7.31 | -3.35 | -3.31 | 1.16 | -1.94 | -14.14 | -9.29 | -8.10 | 12.75 |
Nucleolus | -0.10 | 3.35 | 4.10 | 1.62 | 2.17 | -2.72 | 2.31 | 4.68 | 5.32 | 3.45 | -0.93 | 3.84 | 4.58 | 3.04 | 1.75 |
Nucleus | -0.22 | -13.24 | -15.20 | -13.23 | 10.64 | -5.31 | -8.10 | -0.17 | 3.28 | 7.48 | -4.22 | -14.52 | -16.61 | -11.32 | 10.87 |
Peroxisomes | -6.14 | 11.28 | 2.95 | 6.92 | -1.04 | -6.52 | 13.40 | 0.27 | 4.81 | -1.55 | -8.50 | 17.02 | 3.83 | 9.43 | -1.44 |
Vacuole | 2.84 | -9.37 | -12.11 | -14.34 | 4.01 | -12.86 | -14.53 | -5.63 | -3.83 | 3.50 | -6.40 | -15.81 | -16.59 | -11.93 | 8.39 |
Vacuole Periphery | 3.36 | 17.76 | 11.61 | 6.17 | -2.41 | 2.23 | 9.29 | 9.12 | 11.50 | -0.63 | 4.26 | 18.75 | 11.81 | 7.99 | -2.53 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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