Standard name | |
---|---|
Human Ortholog | |
Description | Hydrophilic protein involved in ER/Golgi vesicle trafficking; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 50 | 71 | 78 | 67 | 51 | 58 | 43 | 62 | 43 | 61 | 60 | 82 | 18 | 17 | 24 | 67 | 59 | 67 | 9 | 21 | 56 | 8 | 21 | 27 |
Bud | 3 | 6 | 7 | 6 | 20 | 21 | 19 | 21 | 18 | 22 | 29 | 31 | 1 | 0 | 1 | 3 | 3 | 6 | 0 | 2 | 0 | 0 | 2 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 3 | 0 | 2 | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 4 | 2 | 4 | 12 | 5 | 6 | 4 | 11 | 5 | 9 | 0 | 0 | 2 | 4 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 13 | 37 | 15 | 27 | 31 | 28 | 32 | 23 | 15 | 7 | 7 | 10 | 3 | 5 | 7 | 14 | 18 | 16 | 1 | 13 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 43 | 81 | 67 | 46 | 56 | 105 | 75 | 57 | 57 | 74 | 62 | 58 | 31 | 41 | 33 | 8 | 7 | 8 | 10 | 20 | 15 | 5 | 17 | 27 |
Endosome | 9 | 1 | 12 | 8 | 41 | 15 | 18 | 38 | 11 | 16 | 12 | 25 | 4 | 7 | 2 | 13 | 8 | 8 | 14 | 29 | 18 | 6 | 21 | 17 |
Golgi | 156 | 121 | 329 | 288 | 243 | 177 | 91 | 140 | 103 | 143 | 105 | 182 | 72 | 103 | 134 | 143 | 141 | 148 | 219 | 190 | 383 | 95 | 191 | 234 |
Mitochondria | 101 | 108 | 257 | 220 | 329 | 631 | 690 | 717 | 535 | 707 | 657 | 665 | 34 | 8 | 26 | 31 | 27 | 32 | 9 | 17 | 4 | 5 | 11 | 12 |
Nucleus | 1 | 0 | 0 | 0 | 1 | 1 | 3 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 1 | 5 | 15 | 5 | 7 | 16 | 6 | 15 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 |
Nucleolus | 0 | 0 | 3 | 0 | 4 | 9 | 43 | 86 | 20 | 29 | 19 | 39 | 0 | 2 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 4 | 1 | 3 | 2 | 9 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 3 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 2 | 5 | 4 | 5 | 0 | 1 | 0 | 1 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 9 | 15 | 3 | 5 | 24 | 22 | 19 | 25 | 12 | 13 | 10 | 20 | 8 | 7 | 10 | 14 | 13 | 7 | 8 | 24 | 1 | 1 | 6 | 4 |
Unique Cell Count | 230 | 275 | 450 | 417 | 536 | 779 | 824 | 921 | 619 | 823 | 746 | 834 | 119 | 151 | 168 | 208 | 189 | 210 | 276 | 326 | 484 | 129 | 279 | 330 |
Labelled Cell Count | 386 | 440 | 776 | 669 | 811 | 1088 | 1057 | 1190 | 829 | 1111 | 982 | 1157 | 172 | 192 | 240 | 300 | 282 | 304 | 276 | 326 | 484 | 129 | 279 | 330 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.4 | 11.0 | 12.1 | 11.5 | 10.6 | 9.4 | 9.3 | 9.8 | 9.8 | 9.8 | 10.0 | 10.2 | 11.9 | 11.9 | 12.6 | 14.8 | 14.4 | 14.2 | 11.1 | 11.3 | 12.0 |
Std Deviation (1e-4) | 1.8 | 1.9 | 1.5 | 1.9 | 1.7 | 1.5 | 1.7 | 1.8 | 1.7 | 1.6 | 1.8 | 2.0 | 1.7 | 1.8 | 1.7 | 2.9 | 3.1 | 2.7 | 1.7 | 1.7 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.19 | -0.36 | -0.37 | -0.3 | -0.31 | -0.3 | -0.27 | -0.24 | -0.02 | -0.02 | 0.06 | 0.29 | 0.25 | 0.23 | -0.12 | -0.09 | -0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.5 | -3.6 | -5.3 | -7.0 | -6.1 | -5.3 | -5.4 | -4.9 | -3.9 | -0.6 | -1.8 | -0.9 | 4.3 | 3.9 | 4.2 |
Bud | -0.1 | 2.1 | 1.3 | 0.9 | 0.9 | 1.4 | 1.3 | 2.3 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 1.0 | 0 | 0 | 0 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.2 | 1.8 | 0.2 | 0.5 | -0.9 | -0.9 | -3.2 | -3.0 | -2.6 | 0 | 0 | 0.5 | 2.0 | 3.2 | 2.4 |
Endoplasmic Reticulum | -1.7 | -2.1 | -0.7 | -3.1 | -5.3 | -2.9 | -3.2 | -3.6 | -4.6 | 2.9 | 3.4 | 1.4 | -4.1 | -4.0 | -4.2 |
Endosome | -0.7 | 3.5 | -0.9 | -0.5 | 1.4 | -1.0 | -0.8 | -1.3 | 0.3 | 0 | 0 | 0 | 2.2 | 1.0 | 0.8 |
Golgi | -1.3 | -8.8 | -17.3 | -22.5 | -21.2 | -18.6 | -19.7 | -20.6 | -17.9 | -2.7 | -1.2 | 1.7 | -1.2 | 0.4 | -0.7 |
Mitochondria | -1.3 | 1.4 | 9.0 | 10.4 | 8.0 | 10.8 | 11.5 | 12.2 | 8.6 | -5.5 | -11.1 | -9.2 | -10.1 | -9.9 | -10.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 2.4 | 0 | 0 | 2.6 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 4.2 | 6.1 | 2.9 | 3.1 | 2.3 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.7 | 2.6 | 2.1 | 2.5 | 1.7 | 1.4 | 0 | 2.2 | 0 | 0 | 0 | 4.6 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.8324 | 7.5643 | 7.7682 | 6.3519 | 6.535 | 7.222 | 9.6036 | 10.4856 | 10.0989 | 9.5688 | 8.5705 | 10.0539 | 7.843 | 8.9443 | 8.2282 | 6.9489 | 8.7131 | 8.3607 |
Actin | 0.0528 | 0.0255 | 0.0182 | 0.0104 | 0.0017 | 0.0037 | 0.037 | 0.0184 | 0.011 | 0.0518 | 0.0079 | 0.0087 | 0.1199 | 0.0979 | 0.0446 | 0.0248 | 0.001 | 0.0569 |
Bud | 0.0005 | 0.0004 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0017 | 0.0007 | 0.0268 | 0.0001 | 0.0007 |
Bud Neck | 0.0007 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0.0005 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0014 | 0.0015 | 0.001 | 0.0008 | 0.0001 | 0.0007 |
Bud Periphery | 0.0006 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0017 | 0.0006 | 0.0863 | 0.0001 | 0.0009 |
Bud Site | 0.0015 | 0.0051 | 0.0004 | 0.0008 | 0.0001 | 0.0001 | 0.0011 | 0.0024 | 0.0003 | 0.0007 | 0.0005 | 0.0001 | 0.0038 | 0.024 | 0.0299 | 0.0017 | 0.0001 | 0.0007 |
Cell Periphery | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0004 | 0.0002 | 0.0006 | 0 | 0.0001 |
Cytoplasm | 0.011 | 0.0057 | 0.011 | 0.0035 | 0.0016 | 0.002 | 0.0041 | 0.0089 | 0.0029 | 0.0025 | 0.0025 | 0.0015 | 0.0063 | 0.0044 | 0.0042 | 0.0083 | 0.0023 | 0.0013 |
Cytoplasmic Foci | 0.0583 | 0.0772 | 0.0619 | 0.0506 | 0.0394 | 0.0304 | 0.0596 | 0.0654 | 0.0445 | 0.068 | 0.0533 | 0.0378 | 0.0709 | 0.0697 | 0.0415 | 0.0273 | 0.0838 | 0.0291 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0006 | 0.0003 | 0.0001 | 0 | 0 | 0.0002 |
Endoplasmic Reticulum | 0.0081 | 0.0025 | 0.0048 | 0.0013 | 0.0126 | 0.0051 | 0.0095 | 0.0021 | 0.0038 | 0.0023 | 0.0022 | 0.0016 | 0.0049 | 0.0022 | 0.0015 | 0.0059 | 0.0014 | 0.0028 |
Endosome | 0.219 | 0.2318 | 0.1869 | 0.1918 | 0.2413 | 0.1544 | 0.1874 | 0.1689 | 0.1627 | 0.2556 | 0.1958 | 0.1244 | 0.198 | 0.1916 | 0.1595 | 0.3265 | 0.254 | 0.141 |
Golgi | 0.5704 | 0.6216 | 0.6922 | 0.6488 | 0.6588 | 0.7627 | 0.6175 | 0.7129 | 0.729 | 0.5785 | 0.6701 | 0.7958 | 0.5003 | 0.5374 | 0.6674 | 0.4375 | 0.6306 | 0.7253 |
Lipid Particles | 0.0211 | 0.0068 | 0.0039 | 0.0013 | 0.0015 | 0.0092 | 0.0214 | 0.0037 | 0.0039 | 0.0204 | 0.027 | 0.0032 | 0.0221 | 0.0077 | 0.0023 | 0.0026 | 0.0039 | 0.0053 |
Mitochondria | 0.0309 | 0.0081 | 0.0122 | 0.0847 | 0.0216 | 0.0206 | 0.0343 | 0.0128 | 0.0341 | 0.0081 | 0.0236 | 0.0228 | 0.0233 | 0.0419 | 0.0137 | 0.0176 | 0.0178 | 0.0281 |
None | 0.0036 | 0.0001 | 0.0023 | 0.0003 | 0.0001 | 0.0003 | 0.0024 | 0 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0086 | 0.0024 | 0.0282 | 0.0006 | 0 | 0.0002 |
Nuclear Periphery | 0.0029 | 0.0002 | 0.0004 | 0.0002 | 0.012 | 0.0051 | 0.0072 | 0.0001 | 0.0002 | 0.0002 | 0.0018 | 0.0001 | 0.0076 | 0.0002 | 0.0002 | 0.0019 | 0.0002 | 0.0008 |
Nucleolus | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0006 | 0.0004 | 0 | 0.0001 | 0 | 0 |
Nucleus | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0008 | 0 | 0 | 0 | 0.0002 | 0 | 0.0008 | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0002 |
Peroxisomes | 0.0077 | 0.0113 | 0.0017 | 0.0012 | 0.0004 | 0.0009 | 0.0074 | 0.0015 | 0.0038 | 0.0073 | 0.0077 | 0.0015 | 0.0165 | 0.0089 | 0.0012 | 0.0007 | 0.0014 | 0.002 |
Punctate Nuclear | 0.0038 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0005 | 0.0032 | 0.0001 | 0.0001 | 0.0004 | 0.0027 | 0.0001 | 0.0074 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 |
Vacuole | 0.003 | 0.0012 | 0.0011 | 0.0013 | 0.003 | 0.0013 | 0.0024 | 0.0006 | 0.001 | 0.001 | 0.0013 | 0.0005 | 0.0023 | 0.0031 | 0.0013 | 0.0203 | 0.0009 | 0.0011 |
Vacuole Periphery | 0.0027 | 0.0014 | 0.0015 | 0.002 | 0.0054 | 0.0025 | 0.0028 | 0.001 | 0.0019 | 0.0021 | 0.0021 | 0.0013 | 0.0029 | 0.0021 | 0.0016 | 0.0091 | 0.002 | 0.0022 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 42.7356 | 37.4636 | 24.5075 | 33.9602 | 43.2544 | 40.783 | 33.1444 | 31.6211 | 30.7292 | 46.5841 |
Translational Efficiency | 1.3911 | 1.0604 | 1.3204 | 0.9777 | 0.9869 | 1.1595 | 1.1295 | 0.932 | 1.0075 | 0.9536 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1743 | 1320 | 717 | 1196 | 1486 | 1147 | 1704 | 1965 | 3229 | 2467 | 2421 | 3161 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1027.60 | 1179.27 | 1430.66 | 1589.21 | 974.21 | 1112.87 | 1266.49 | 1537.07 | 1003.03 | 1148.40 | 1315.11 | 1556.80 |
Standard Deviation | 169.43 | 194.93 | 204.07 | 289.46 | 199.63 | 162.16 | 238.79 | 222.40 | 185.86 | 183.45 | 241.01 | 251.18 |
Intensity Change Log 2 | — | 0.198615 | 0.477402 | 0.629031 | — | 0.191980 | 0.378531 | 0.657878 | — | 0.195390 | 0.430131 | 0.643142 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.004606 | 0.005476 | 0.007389 | 0.027806 | 0.003443 | 0.005566 | 0.005684 | 0.006443 | 0.004071 | 0.005518 | 0.006189 | 0.014526 |
Bud Neck | 0.000092 | 0.000134 | 0.000634 | 0.003483 | 0.000264 | 0.000134 | 0.000227 | 0.000227 | 0.000171 | 0.000134 | 0.000347 | 0.001459 |
Bud Site | 0.001184 | 0.002041 | 0.003745 | 0.035078 | 0.002080 | 0.001590 | 0.002201 | 0.003714 | 0.001596 | 0.001832 | 0.002658 | 0.015581 |
Cell Periphery | 0.000204 | 0.000217 | 0.000331 | 0.002708 | 0.000279 | 0.000273 | 0.000436 | 0.000452 | 0.000239 | 0.000243 | 0.000405 | 0.001306 |
Cytoplasm | 0.000425 | 0.000609 | 0.000497 | 0.009376 | 0.001378 | 0.000756 | 0.000489 | 0.000833 | 0.000864 | 0.000677 | 0.000492 | 0.004065 |
Cytoplasmic Foci | 0.163138 | 0.169522 | 0.071468 | 0.081934 | 0.178188 | 0.176048 | 0.074582 | 0.115459 | 0.170064 | 0.172557 | 0.073660 | 0.102775 |
Eisosomes | 0.000108 | 0.000104 | 0.000097 | 0.000366 | 0.000090 | 0.000101 | 0.000179 | 0.000192 | 0.000100 | 0.000102 | 0.000155 | 0.000258 |
Endoplasmic Reticulum | 0.005336 | 0.009673 | 0.017638 | 0.023571 | 0.011963 | 0.008533 | 0.017544 | 0.008896 | 0.008386 | 0.009143 | 0.017572 | 0.014448 |
Endosome | 0.140395 | 0.126212 | 0.109337 | 0.114731 | 0.159059 | 0.149908 | 0.109259 | 0.164326 | 0.148984 | 0.137229 | 0.109282 | 0.145561 |
Golgi | 0.655911* | 0.662557* | 0.733123* | 0.597718* | 0.594424* | 0.631505* | 0.729650* | 0.641151* | 0.627614* | 0.648120* | 0.730679* | 0.624718* |
Lipid Particles | 0.010174 | 0.009239 | 0.018395 | 0.057874 | 0.018468 | 0.007080 | 0.035762 | 0.023376 | 0.013991 | 0.008235 | 0.030619 | 0.036429 |
Mitochondria | 0.006741 | 0.002744 | 0.000493 | 0.002176 | 0.007230 | 0.003190 | 0.000686 | 0.000865 | 0.006966 | 0.002951 | 0.000629 | 0.001361 |
Mitotic Spindle | 0.000113 | 0.000509 | 0.001877 | 0.004439 | 0.000374 | 0.000698 | 0.000440 | 0.001300 | 0.000233 | 0.000597 | 0.000866 | 0.002488 |
None | 0.000039 | 0.000047 | 0.000024 | 0.001265 | 0.000164 | 0.000022 | 0.000042 | 0.000027 | 0.000096 | 0.000035 | 0.000037 | 0.000495 |
Nuclear Periphery | 0.000033 | 0.000076 | 0.000364 | 0.000160 | 0.000078 | 0.000173 | 0.000108 | 0.000162 | 0.000054 | 0.000121 | 0.000184 | 0.000161 |
Nuclear Periphery Foci | 0.000239 | 0.000538 | 0.002379 | 0.002195 | 0.001690 | 0.000763 | 0.001452 | 0.002150 | 0.000907 | 0.000642 | 0.001727 | 0.002167 |
Nucleolus | 0.000003 | 0.000010 | 0.000025 | 0.000033 | 0.000107 | 0.000005 | 0.000002 | 0.000003 | 0.000051 | 0.000008 | 0.000009 | 0.000014 |
Nucleus | 0.000014 | 0.000026 | 0.000039 | 0.000593 | 0.000399 | 0.000026 | 0.000014 | 0.000015 | 0.000191 | 0.000026 | 0.000021 | 0.000234 |
Peroxisomes | 0.000598 | 0.000377 | 0.000332 | 0.000946 | 0.000753 | 0.000504 | 0.000440 | 0.000576 | 0.000670 | 0.000436 | 0.000408 | 0.000716 |
Vacuole | 0.003253 | 0.004296 | 0.021777 | 0.025542 | 0.007940 | 0.005330 | 0.012580 | 0.021994 | 0.005410 | 0.004776 | 0.015304 | 0.023337 |
Vacuole Periphery | 0.007394 | 0.005591 | 0.010037 | 0.008005 | 0.011628 | 0.007795 | 0.008222 | 0.007837 | 0.009342 | 0.006616 | 0.008760 | 0.007900 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.04 | -2.14 | -7.97 | -7.58 | -6.55 | -1.97 | -2.06 | -2.66 | -0.70 | -0.59 | -2.15 | -2.59 | -8.01 | -6.65 | -5.79 |
Bud Neck | -1.16 | -3.97 | -9.83 | -9.69 | -7.94 | 2.10 | 0.51 | 0.39 | -2.12 | -0.11 | 1.06 | -3.26 | -9.39 | -9.69 | -7.98 |
Bud Site | -1.58 | -4.42 | -11.65 | -11.25 | -10.69 | 1.59 | -0.40 | -2.89 | -3.80 | -2.59 | -0.74 | -3.75 | -11.80 | -11.39 | -10.82 |
Cell Periphery | -0.26 | -2.77 | -8.42 | -8.33 | -7.99 | 0.09 | -3.39 | -2.70 | -2.06 | -0.20 | -0.10 | -4.81 | -8.82 | -8.52 | -7.40 |
Cytoplasm | -1.57 | -1.19 | -6.44 | -6.31 | -6.39 | 1.52 | 2.64 | 1.49 | -0.35 | -3.08 | 0.92 | 2.31 | -5.87 | -6.24 | -6.66 |
Cytoplasmic Foci | -1.58 | 24.69 | 21.89 | 20.80 | -2.84 | 0.45 | 27.67 | 15.33 | 14.57 | -13.35 | -0.81 | 38.16 | 24.42 | 23.46 | -12.17 |
Eisosomes | 0.44 | 1.08 | -4.53 | -4.60 | -4.72 | -0.82 | -3.28 | -2.34 | -2.07 | -0.25 | -0.29 | -2.85 | -4.56 | -4.47 | -2.63 |
Endoplasmic Reticulum | -3.01 | -5.29 | -8.10 | -5.72 | -1.89 | 1.99 | -2.88 | 1.90 | -0.26 | 4.87 | -0.68 | -6.46 | -4.84 | -4.01 | 2.03 |
Endosome | 3.41 | 6.19 | 6.50 | 3.00 | -0.84 | 1.78 | 10.96 | -1.07 | -3.00 | -13.17 | 3.59 | 12.41 | 1.13 | -2.43 | -11.16 |
Golgi | -0.95 | -8.16 | 7.01 | 7.40 | 12.23 | -4.46 | -17.48 | -5.79 | -1.12 | 11.97 | -3.79 | -18.13 | 1.07 | 4.40 | 17.23 |
Lipid Particles | 0.56 | -3.36 | -12.10 | -12.40 | -9.13 | 5.15 | -5.97 | -1.98 | -7.89 | 4.39 | 4.22 | -8.28 | -10.73 | -14.29 | -2.37 |
Mitochondria | 3.65 | 6.55 | 4.37 | 0.75 | -4.10 | 3.35 | 6.71 | 6.36 | 3.00 | -0.58 | 4.95 | 9.24 | 7.81 | 3.12 | -2.96 |
Mitotic Spindle | -1.39 | -2.16 | -5.25 | -4.56 | -2.30 | -1.56 | -0.29 | -3.48 | -1.92 | -2.22 | -2.02 | -2.04 | -6.38 | -4.89 | -3.50 |
None | -0.38 | 1.54 | -2.00 | -1.98 | -2.02 | 3.13 | 2.67 | 2.98 | -0.82 | 1.60 | 2.49 | 2.72 | -1.72 | -1.98 | -1.98 |
Nuclear Periphery | -1.24 | -3.26 | -9.06 | -2.47 | 1.92 | -1.10 | -1.31 | -1.81 | 0.11 | -1.23 | -1.52 | -3.98 | -3.89 | -0.86 | 0.48 |
Nuclear Periphery Foci | -1.84 | -4.62 | -7.94 | -5.86 | 0.20 | 1.33 | 0.35 | -0.73 | -3.42 | -1.98 | 0.79 | -2.32 | -3.70 | -6.02 | -1.68 |
Nucleolus | -0.83 | -0.99 | -5.42 | -2.70 | -0.47 | 1.85 | 1.91 | 1.90 | 0.78 | -1.66 | 1.68 | 1.59 | 1.37 | -1.61 | -0.95 |
Nucleus | -0.89 | -1.14 | -3.41 | -3.35 | -3.28 | 1.17 | 1.20 | 1.20 | 0.89 | -0.72 | 1.12 | 1.15 | -0.70 | -3.26 | -3.31 |
Peroxisomes | 1.19 | 1.37 | -1.70 | -3.72 | -3.81 | 1.69 | 1.93 | 0.88 | -0.40 | -0.72 | 1.93 | 1.99 | -0.29 | -2.23 | -2.27 |
Vacuole | -1.61 | -9.49 | -12.51 | -11.58 | -2.14 | 2.63 | -4.22 | -9.21 | -10.79 | -5.82 | 1.09 | -10.86 | -15.93 | -15.98 | -6.31 |
Vacuole Periphery | 2.57 | -2.24 | -0.88 | -3.38 | 1.64 | 3.02 | 3.18 | 3.61 | -0.05 | 0.55 | 3.90 | 0.80 | 2.22 | -2.20 | 1.38 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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