ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.96 0.93 0.95 0.86 0.88 0.78 0.72 0.75 0.62 0.64 0.99 0.99 0.99 0.9 0.83 0.91 0.88 0.86 0.65 0.77 0.81 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.17 0.05 0.34 0.33 0.35 0.52 0.47 0.62 0.56 0 0 0 0 0.08 0.07 0 0.05 0.18 0.08 0.06 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 3 2 0 1 1
Bud 0 2 2 2 3 4 11 8 12 10 13 15 0 2 0 0 0 2 1 3 0 0 2 1
Bud Neck 1 2 1 0 2 0 4 4 2 7 0 1 0 0 1 1 1 2 0 2 0 0 0 1
Bud Site 0 0 0 0 0 0 1 5 0 1 2 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 1 3 2 1 2 4 0 2 2 1 1 0 0 1 1 1 0 2 1 0 0 1
Cytoplasm 436 409 155 184 333 228 369 419 216 308 152 225 183 290 341 123 30 89 387 351 100 67 145 188
Endoplasmic Reticulum 0 0 1 2 1 0 0 0 1 0 0 0 0 0 0 9 2 9 0 2 2 0 0 0
Endosome 0 0 0 0 3 1 0 1 0 0 0 0 0 0 0 6 1 0 3 6 2 2 1 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 5 3 1 0 0 0 0
Mitochondria 14 4 13 34 19 91 138 185 157 191 153 196 0 2 1 5 3 7 18 21 27 7 10 7
Nucleus 1 1 0 0 0 1 0 1 1 1 1 1 1 1 0 4 0 0 0 1 0 0 0 0
Nuclear Periphery 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 2 1 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 1 2 0 0 1 2 13 19 4 8 7 16 0 0 0 0 2 2 5 1 2 0 1 1
Vac/Vac Membrane 1 1 0 0 3 0 0 5 4 4 0 0 0 0 1 4 0 0 7 5 2 2 9 3
Unique Cell Count 448 416 161 197 349 264 417 535 300 410 247 350 184 292 344 136 36 98 438 409 155 88 180 210
Labelled Cell Count 456 424 174 228 370 330 540 652 398 533 330 461 185 295 344 154 40 119 438 409 155 88 180 210


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.0 3.2 3.1 3.6 2.8 2.8 3.0 2.6 2.7 2.5 2.4 4.4 4.4 4.4 5.7 5.8 5.5 4.0 4.4 4.4
Std Deviation (1e-4) 0.6 1.0 1.3 3.0 1.8 1.5 2.3 1.9 2.1 1.0 1.3 0.8 1.4 1.7 1.5 1.6 2.0 2.3 1.2 1.3 1.5
Intensity Change (Log2) – – – -0.03 0.18 -0.19 -0.19 -0.1 -0.29 -0.28 -0.35 -0.45 0.45 0.46 0.44 0.82 0.86 0.76 0.31 0.44 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.3922 -0.6147 -0.5182 -0.519 -0.0276 -0.2928 -0.7945 -1.215 -0.9589 -0.8238 -0.7814 -1.1637 0.2229 1.0216 0.6062 0.8878 0.7571 0.6732
Actin 0.0566 0.0049 0.0103 0.0244 0.0519 0.0216 0.0239 0.0002 0.0129 0.0026 0.0275 0.0027 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003
Bud 0.0002 0.0002 0.0004 0.001 0.0019 0.0004 0.0015 0.003 0.0032 0.0174 0.0045 0.001 0 0.0002 0.0001 0.0001 0 0
Bud Neck 0.0007 0.0001 0.0005 0.0004 0.0077 0.0015 0.0075 0.0001 0.0013 0.0008 0.0032 0.0004 0 0.0001 0.0002 0.0003 0.0001 0.0002
Bud Periphery 0.0003 0.0002 0.0006 0.0017 0.0028 0.0007 0.0067 0.0028 0.0039 0.0074 0.0146 0.0007 0.0001 0.0002 0.0002 0 0 0.0003
Bud Site 0.001 0.0029 0.0093 0.0036 0.0206 0.0003 0.0106 0.0058 0.0091 0.0032 0.018 0.0004 0.0001 0.0006 0.0004 0.0002 0 0
Cell Periphery 0.0002 0.0001 0.0006 0.0002 0.0013 0.0001 0.0007 0.0003 0.0002 0.0003 0.0006 0.0001 0 0.0001 0.0001 0 0 0.0001
Cytoplasm 0.0217 0.0344 0.0263 0.0172 0.0082 0.013 0.0208 0.0359 0.0276 0.0254 0.062 0.0578 0.0322 0.0732 0.0333 0.0362 0.0452 0.0582
Cytoplasmic Foci 0.0387 0.0022 0.006 0.0215 0.0281 0.0023 0.0363 0.0021 0.0159 0.0152 0.0207 0.0078 0.0016 0.0053 0.0039 0.0167 0.0015 0.0035
Eisosomes 0.0004 0.0003 0.0002 0.0005 0.0011 0.0001 0.0009 0.0001 0.0003 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0055 0.0004 0.0035 0.0015 0.0038 0.0025 0.0038 0.0003 0.001 0.0005 0.0014 0.0007 0.0004 0.0005 0.0003 0.0002 0.0004 0.0002
Endosome 0.0334 0.001 0.0108 0.0123 0.0874 0.0148 0.0265 0.0001 0.0243 0.0202 0.0378 0.0045 0.0007 0.0008 0.0023 0.0057 0.0013 0.0023
Golgi 0.0127 0.0026 0.0069 0.0111 0.0289 0.0168 0.0082 0 0.0106 0.0109 0.024 0.0026 0 0.0001 0.0002 0.0002 0 0.0001
Lipid Particles 0.0264 0.0004 0.0028 0.0122 0.0333 0.0009 0.0211 0.0001 0.0042 0.0372 0.0377 0.0027 0.0001 0.0001 0.0004 0.0008 0.0002 0
Mitochondria 0.0037 0.005 0.0078 0.0127 0.0557 0.0146 0.0169 0.0002 0.0104 0.076 0.0372 0.0042 0.0001 0.0003 0.001 0.0002 0.0002 0.0003
None 0.7562 0.9439 0.9021 0.8625 0.6238 0.9037 0.764 0.9474 0.8565 0.7332 0.6765 0.9047 0.9623 0.9134 0.9541 0.9378 0.9452 0.9332
Nuclear Periphery 0.0162 0.0001 0.0018 0.001 0.0044 0.0038 0.0075 0.0001 0.0013 0.0012 0.0011 0.0013 0.0003 0.0003 0.0003 0.0002 0.0007 0.0001
Nucleolus 0.0003 0.0001 0.0002 0.0002 0.0029 0.0001 0.0026 0.0001 0.0003 0.0023 0.0013 0.0003 0.0003 0.0003 0.0002 0.0001 0.0006 0.0001
Nucleus 0.0023 0.0001 0.0005 0.0003 0.0031 0.0005 0.0046 0.0004 0.0008 0.0077 0.0012 0.0016 0.0006 0.0023 0.0006 0.0005 0.0027 0.0003
Peroxisomes 0.0166 0.0007 0.003 0.013 0.0106 0.0003 0.0182 0.0001 0.0124 0.0199 0.0171 0.0031 0 0.0003 0.001 0.0003 0 0.0001
Punctate Nuclear 0.0034 0.0002 0.0003 0.0016 0.003 0.0008 0.006 0.0004 0.0011 0.0087 0.0053 0.0025 0.0006 0.0012 0.0006 0.0003 0.0009 0.0003
Vacuole 0.0027 0.0002 0.0051 0.0006 0.0156 0.0005 0.0082 0.0003 0.0017 0.0079 0.0049 0.0007 0.0003 0.0005 0.0005 0.0003 0.0006 0.0004
Vacuole Periphery 0.0008 0.0001 0.001 0.0004 0.0036 0.0006 0.0035 0 0.0007 0.0016 0.0031 0.0003 0 0.0001 0.0001 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2068 1425 386 1496 2043 1893 2038 1560 4111 3318 2424 3056

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 631.86 714.21 839.10 792.68 614.08 742.90 784.42 767.15 623.02 730.58 793.13 779.65
Standard Deviation 101.51 90.21 88.18 105.76 63.65 91.99 94.13 93.32 85.30 92.33 95.33 100.42
Intensity Change Log 2 — 0.176743 0.409238 0.327134 — 0.274741 0.353200 0.321082 — 0.225875 0.381891 0.324154

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000070 0.000291 0.000228 0.000583 0.000155 0.000737 0.000127 0.000551 0.000112 0.000545 0.000143 0.000567
Bud Neck 0.016626 0.045252 0.003364 0.011412 0.019661 0.054671 0.006572 0.008005 0.018134 0.050625 0.006061 0.009673
Bud Site 0.002467 0.017933 0.012532 0.032358 0.004189 0.015987 0.012187 0.023237 0.003323 0.016823 0.012242 0.027702
Cell Periphery 0.000481 0.000139 0.000119 0.000289 0.000156 0.000234 0.000150 0.000174 0.000319 0.000193 0.000145 0.000230
Cytoplasm 0.508348* 0.386560* 0.209540* 0.425090* 0.431704* 0.354252* 0.415749* 0.483937* 0.470259* 0.368127* 0.382912* 0.455129*
Cytoplasmic Foci 0.163124 0.225198* 0.009616 0.021749 0.219992* 0.246194* 0.010489 0.027008 0.191385* 0.237177* 0.010350 0.024433
Eisosomes 0.001154 0.000128 0.000050 0.000054 0.000237 0.000195 0.000033 0.000050 0.000698 0.000166 0.000036 0.000052
Endoplasmic Reticulum 0.001381 0.000686 0.003914 0.002752 0.000538 0.000646 0.001208 0.001430 0.000962 0.000663 0.001639 0.002077
Endosome 0.006378 0.020858 0.001658 0.006377 0.006238 0.029775 0.002457 0.003778 0.006308 0.025945 0.002330 0.005050
Golgi 0.001977 0.003897 0.000048 0.004970 0.001861 0.005534 0.000727 0.001495 0.001919 0.004831 0.000619 0.003196
Lipid Particles 0.010144 0.006564 0.001659 0.001741 0.012239 0.009938 0.000855 0.001398 0.011186 0.008489 0.000983 0.001566
Mitochondria 0.002801 0.013336 0.000334 0.002574 0.009734 0.020001 0.001054 0.001115 0.006246 0.017138 0.000940 0.001829
Mitotic Spindle 0.000341 0.006326 0.000529 0.026254 0.001780 0.008432 0.006919 0.009025 0.001056 0.007527 0.005902 0.017459
None 0.019495 0.001864 0.004550 0.007818 0.002156 0.002361 0.005947 0.003454 0.010878 0.002147 0.005725 0.005590
Nuclear Periphery 0.000426 0.000614 0.001518 0.002098 0.000534 0.000637 0.001478 0.001354 0.000479 0.000627 0.001485 0.001718
Nuclear Periphery Foci 0.000539 0.000361 0.000752 0.001272 0.000132 0.000304 0.000286 0.000481 0.000337 0.000329 0.000360 0.000868
Nucleolus 0.001522 0.001682 0.000512 0.001076 0.001143 0.002270 0.000533 0.000392 0.001334 0.002017 0.000530 0.000727
Nucleus 0.221008* 0.167078* 0.710794* 0.335199* 0.247250* 0.144365* 0.491463* 0.335337* 0.234050* 0.154120* 0.526389* 0.335270*
Peroxisomes 0.003847 0.016540 0.000221 0.001274 0.005988 0.016383 0.000268 0.003530 0.004911 0.016451 0.000260 0.002426
Vacuole 0.037413 0.082655 0.037941 0.110710 0.033637 0.083802 0.040897 0.093447 0.035537 0.083310 0.040426 0.101898
Vacuole Periphery 0.000459 0.002038 0.000121 0.004349 0.000676 0.003286 0.000599 0.000801 0.000567 0.002750 0.000523 0.002538

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.25 -8.16 -4.61 -1.70 -1.45 -1.22 -0.32 -1.47 0.74 -1.43 -1.62 -2.39 -3.50 0.50 -2.55
Bud Neck -9.41 9.51 5.09 12.22 -4.37 -12.19 9.68 9.88 17.51 0.26 -15.51 11.69 10.43 21.17 -1.69
Bud Site -8.46 -1.76 -7.79 -0.64 -2.17 -7.59 -3.91 -4.95 0.61 -1.75 -11.49 -4.87 -9.03 -0.12 -3.42
Cell Periphery 5.97 5.85 4.79 -1.73 -1.54 -1.91 0.74 0.97 3.03 0.47 3.55 5.67 4.80 1.52 -1.19
Cytoplasm 13.41 24.73 20.12 5.37 -10.38 9.30 8.55 4.80 -3.71 -3.08 16.62 19.62 17.38 1.05 -3.51
Cytoplasmic Foci -8.87 37.61 36.41 36.41 -4.20 -3.77 45.41 42.02 43.54 -8.89 -9.31 58.52 55.01 56.86 -9.41
Eisosomes 5.24 5.54 5.57 12.00 0.80 2.41 12.36 11.21 16.83 -7.19 5.37 6.59 6.43 20.25 -7.60
Endoplasmic Reticulum 2.62 -8.62 -8.26 -16.07 3.68 -1.06 -12.25 -12.57 -11.82 -2.18 2.08 -9.81 -12.86 -19.87 -3.26
Endosome -9.52 8.06 4.39 11.58 -3.38 -15.17 6.75 6.11 18.10 -0.99 -17.84 9.32 7.43 21.30 -2.62
Golgi -3.38 6.59 0.18 2.36 -2.60 -6.02 3.57 2.71 7.36 -0.54 -6.84 4.75 1.54 6.59 -1.89
Lipid Particles 3.61 10.24 11.03 7.49 1.60 2.35 14.08 13.52 13.39 -4.49 3.85 18.10 17.40 15.12 -4.90
Mitochondria -6.52 5.28 2.16 7.48 -6.11 -5.15 7.22 7.38 12.25 0.96 -8.39 8.17 7.64 14.23 -2.09
Mitotic Spindle -4.62 -1.32 -8.17 -4.64 -8.00 -4.33 -3.68 -3.24 0.84 -0.18 -6.34 -4.58 -8.52 -3.00 -4.35
None 7.89 6.83 6.04 -5.29 -1.92 -0.40 -4.33 -2.78 -1.56 3.02 7.58 5.00 5.65 -5.12 0.82
Nuclear Periphery -1.98 -15.67 -25.46 -18.96 -10.77 -0.88 -20.81 -19.03 -11.77 -1.31 -1.91 -26.41 -31.49 -21.43 -8.56
Nuclear Periphery Foci 1.68 -3.79 -4.91 -6.66 -0.26 -1.73 -7.24 -12.32 -3.03 -3.39 0.17 -3.51 -7.97 -6.93 -5.19
Nucleolus -0.51 6.96 3.00 3.17 -2.04 -2.99 3.88 5.04 5.53 2.03 -2.81 7.16 5.43 6.14 -1.04
Nucleus 7.75 -32.47 -19.40 -23.84 19.61 16.49 -34.67 -15.79 -29.02 15.64 17.39 -46.53 -24.93 -37.62 21.69
Peroxisomes -9.75 8.51 7.07 12.85 -2.10 -9.65 11.93 4.77 11.95 -1.96 -13.94 14.40 7.37 17.78 -2.37
Vacuole -13.34 -4.91 -25.27 -15.69 -13.11 -16.52 -10.92 -23.62 -13.53 -16.40 -21.02 -11.67 -34.51 -20.78 -23.54
Vacuole Periphery -3.98 7.79 -2.11 0.39 -2.65 -7.95 2.12 1.80 8.18 0.07 -8.65 1.94 -1.63 4.30 -2.14
ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
Localization
Cell Percentages cytoplasm (30%), nucleus (3%), mixed (39%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YDR193W

YDR193W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ydr193w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available