Standard name
Human Ortholog
Description RNA-binding protein; involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes; putative regulatory subunit of type 1 protein phosphatase Glc7p, required for actomyosin ring formation, and for timely dephosphorylation and release of Bnr1p from the division site; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0.07 0 0 0 0 0 0 0 0 0.11 0.1 0.15 0.1 0.09 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0.08 0.18 0.27 0.15 0.23 0.32 0.22 0.33 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.91 0.95 0.94 0.88 0.87 0.82 0.75 0.74 0.81 0.71 0.68 0.66 0.83 0.79 0.75 0.85 0.84 0.87 0.87 0.93 0.88 0.82 0.72 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.07 0.09 0.1 0.12 0.16 0.23 0.28 0.25 0.39 0.42 0.36 0.05 0.14 0.13 0 0 0.05 0 0 0 0.07 0.14 0.14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2 1 2 2 1 0 0 0 0 0 0 0 3 2 2 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 0 1 13 13 3 4 12 5 12 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 0 6 11 6 9 8 18 3 5 8 1 18 13 26 41 22 15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 9 5 2 0 0 1 0 0 0
Endosome 0 1 1 0 1 0 0 0 0 0 0 0 0 0 3 2 3 0 1 0 1 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 4 2 0 0
Mitochondria 7 4 8 6 20 56 124 78 72 143 41 66 2 1 2 16 2 2 1 0 0 0 0 2
Nucleus 111 220 228 132 208 261 345 379 258 316 125 130 141 98 130 354 204 167 107 223 232 51 101 70
Nuclear Periphery 0 1 1 0 2 9 18 4 6 5 1 2 1 1 0 1 1 0 0 0 0 0 0 0
Nucleolus 13 17 22 15 28 52 106 143 81 171 76 72 9 17 22 11 3 9 3 5 9 4 19 15
Peroxisomes 0 1 0 0 1 0 0 0 0 0 0 0 1 3 2 1 0 0 1 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 5 3 0 0 1 0 3 1 1 1 2 0 0 0 0 0 1 0
Vac/Vac Membrane 2 4 4 1 2 1 5 3 1 0 1 1 3 0 1 4 9 7 2 5 5 2 11 17
Unique Cell Count 122 231 242 150 239 317 459 511 318 442 183 198 169 124 173 418 242 191 124 242 264 63 141 111
Labelled Cell Count 140 248 271 165 269 401 627 632 427 654 260 284 179 134 187 442 251 202 124 242 264 63 141 111


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 8.3 7.3 6.9 6.5 5.7 5.1 5.7 5.6 5.1 5.4 5.1 7.8 7.7 7.5 8.4 9.9 10.0 7.1 8.1 8.4
Std Deviation (1e-4) 1.5 1.5 1.5 2.0 1.6 1.3 1.7 1.6 1.3 1.3 1.5 1.2 2.3 1.8 2.2 2.1 2.6 2.2 2.0 1.8 1.6
Intensity Change (Log2) – – – -0.08 -0.18 -0.36 -0.54 -0.36 -0.39 -0.51 -0.45 -0.52 0.09 0.06 0.03 0.2 0.43 0.44 -0.05 0.14 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 2.8 0 0 0 0 0 3.5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.3 0 0.3 0 0.8 0 0 1.1 0 3.5 3.3 4.7 3.5 3.1 2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 2.4 5.3 7.6 4.8 6.5 8.8 6.1 8.4 0 0 0 0.3 -1.9 0
Nucleus -2.2 -2.7 -4.2 -6.2 -6.5 -4.5 -7.0 -7.1 -7.7 -3.6 -4.4 -5.6 -3.7 -3.5 -2.5
Nuclear Periphery 0 0 0 2.7 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.3 0.9 2.5 4.6 5.9 5.0 8.2 7.9 6.9 -1.4 1.4 1.2 -3.7 -3.9 -1.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 1.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9315 3.9722 3.7306 3.396 2.8557 3.5351 3.9174 4.5383 4.103 4.1608 4.0092 4.3878 5.4016 6.0247 5.3954 6.0469 5.1926 5.3694
Actin 0.0068 0 0.0059 0 0.0001 0 0.0007 0.0001 0.0078 0 0.0015 0.0004 0.0437 0 0.0218 0 0.0068 0.0003
Bud 0.0002 0 0.0003 0 0 0 0.0001 0.0001 0.0004 0 0.0003 0 0.0003 0 0.0001 0 0.0001 0.0002
Bud Neck 0.0002 0 0.0005 0 0.0006 0.0005 0.0002 0.0002 0.0004 0 0.001 0.0005 0.0016 0 0.0001 0 0.0002 0.0004
Bud Periphery 0.0003 0 0.0004 0 0.0001 0 0.0002 0.0003 0.0006 0 0.0006 0.0001 0.0007 0 0.0001 0 0.0003 0.0004
Bud Site 0.0006 0 0.0018 0 0.0001 0 0.0005 0.0012 0.0014 0 0.0009 0.0001 0.001 0 0.0005 0 0.0007 0.0006
Cell Periphery 0.0003 0 0.0001 0 0 0 0 0 0.0002 0 0.0001 0 0.0002 0 0 0 0.0001 0.0001
Cytoplasm 0.0075 0 0.0013 0 0.0001 0.0001 0.0006 0 0.0153 0 0.0069 0.0013 0.0017 0 0.0001 0 0.0011 0.0001
Cytoplasmic Foci 0.0182 0 0.0048 0 0 0 0.002 0.0026 0.0169 0 0.0069 0.0006 0.0112 0 0.0005 0 0.0123 0.0006
Eisosomes 0.0002 0 0.0002 0 0 0 0 0 0.0001 0 0 0 0.0002 0 0.0003 0 0.0002 0
Endoplasmic Reticulum 0.0352 0 0.0009 0 0 0 0.0015 0 0.0072 0 0.0027 0.0001 0.001 0 0.0001 0 0.0005 0.0001
Endosome 0.0552 0 0.0093 0 0.0001 0 0.0215 0.0009 0.0374 0 0.0255 0.0012 0.0059 0 0.0004 0 0.0087 0.0032
Golgi 0.0227 0 0.0053 0 0 0 0.0021 0.0009 0.0194 0 0.0024 0.0001 0.0125 0 0.0012 0 0.005 0.0025
Lipid Particles 0.0226 0 0.0051 0 0 0 0.0045 0.007 0.0037 0 0.0021 0.0002 0.0144 0 0.0008 0 0.014 0.0057
Mitochondria 0.0041 0 0.005 0.0001 0.0006 0.0001 0.0015 0.0006 0.004 0.0001 0.0033 0.0002 0.0033 0 0.0148 0 0.0066 0.0082
None 0.0019 0 0.0188 0 0.0001 0 0.0009 0.0001 0.0099 0 0.0009 0.0013 0.0026 0 0.0002 0 0.0013 0
Nuclear Periphery 0.0385 0.0005 0.0061 0.0033 0.0028 0.0003 0.0262 0.0015 0.0166 0.0005 0.0207 0.0039 0.0124 0.0003 0.0004 0.0012 0.0008 0.0002
Nucleolus 0.0135 0.0038 0.0176 0.0178 0.1149 0.0504 0.0159 0.031 0.0071 0.0099 0.1322 0.0504 0.0298 0.0072 0.0055 0.0079 0.0658 0.0418
Nucleus 0.7552 0.9956 0.908 0.9784 0.8775 0.9473 0.8819 0.9389 0.8403 0.9893 0.7794 0.9302 0.8207 0.9923 0.9483 0.9894 0.8657 0.9335
Peroxisomes 0.0075 0 0.0057 0 0 0 0.0004 0.0027 0.0026 0 0.0016 0.0001 0.0241 0 0.0046 0 0.0085 0.0002
Punctate Nuclear 0.0038 0.0001 0.0012 0.0002 0.0024 0.0008 0.0331 0.0116 0.0047 0.0001 0.0079 0.0091 0.0107 0.0001 0.0001 0.0014 0.0008 0.0002
Vacuole 0.0033 0 0.0008 0 0.0002 0.0001 0.0018 0.0002 0.0025 0 0.0019 0.0001 0.0015 0 0.0001 0 0.0003 0.0011
Vacuole Periphery 0.0023 0 0.0009 0 0.0002 0 0.0045 0.0001 0.0015 0 0.001 0.0001 0.0008 0 0.0001 0 0.0002 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.3978 13.5457 12.336 14.3251 14.7348 11.0142 10.5833 13.4457 10.5278 15.16
Translational Efficiency 2.8014 1.9012 1.8885 1.5682 1.795 3.1235 2.1769 1.8865 1.8787 1.7429

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2036 584 461 1675 2028 1634 145 309 4064 2218 606 1984

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 816.48 886.85 1169.97 1219.19 876.64 914.62 1276.95 1243.36 846.50 907.31 1195.57 1222.95
Standard Deviation 111.51 160.89 152.33 256.48 350.00 144.80 150.10 218.77 261.27 149.71 158.51 251.13
Intensity Change Log 2 — 0.119273 0.518982 0.578434 — 0.061188 0.542646 0.504188 — 0.089490 0.531283 0.540469

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000329 0.000888 0.000873 0.000901 0.000280 0.000539 0.000788 0.000898 0.000305 0.000631 0.000853 0.000900
Bud Neck 0.003980 0.007764 0.013007 0.022320 0.005440 0.009356 0.007907 0.016807 0.004709 0.008937 0.011787 0.021462
Bud Site 0.001018 0.003051 0.000915 0.004803 0.000620 0.001282 0.000642 0.002295 0.000820 0.001748 0.000850 0.004412
Cell Periphery 0.000305 0.000088 0.000028 0.000139 0.000184 0.000111 0.000024 0.000081 0.000245 0.000105 0.000027 0.000130
Cytoplasm 0.001755 0.001357 0.000074 0.001583 0.000402 0.000967 0.000040 0.000901 0.001080 0.001070 0.000066 0.001477
Cytoplasmic Foci 0.000464 0.000746 0.000021 0.001033 0.000257 0.000526 0.000015 0.000195 0.000361 0.000584 0.000020 0.000903
Eisosomes 0.000059 0.000041 0.000061 0.000034 0.000040 0.000037 0.000052 0.000044 0.000049 0.000038 0.000059 0.000035
Endoplasmic Reticulum 0.004808 0.004677 0.001926 0.001953 0.003816 0.005171 0.001618 0.001584 0.004313 0.005041 0.001853 0.001895
Endosome 0.000335 0.000497 0.000048 0.000931 0.000178 0.001017 0.000028 0.001014 0.000256 0.000880 0.000043 0.000944
Golgi 0.000150 0.000484 0.000082 0.001338 0.000090 0.000932 0.000051 0.000176 0.000120 0.000814 0.000074 0.001157
Lipid Particles 0.000080 0.001693 0.000034 0.000096 0.000055 0.000248 0.000021 0.000061 0.000068 0.000628 0.000031 0.000091
Mitochondria 0.000747 0.004108 0.001164 0.002815 0.000774 0.001751 0.001111 0.003004 0.000760 0.002371 0.001151 0.002845
Mitotic Spindle 0.000298 0.000539 0.000288 0.008070 0.000517 0.003243 0.000024 0.009300 0.000407 0.002531 0.000225 0.008261
None 0.006654 0.005338 0.000650 0.002447 0.001922 0.002191 0.000628 0.007366 0.004293 0.003019 0.000645 0.003213
Nuclear Periphery 0.000108 0.000111 0.000083 0.001540 0.000123 0.000418 0.000062 0.000278 0.000115 0.000337 0.000078 0.001343
Nuclear Periphery Foci 0.000261 0.000079 0.000027 0.000450 0.000084 0.001176 0.000006 0.000144 0.000172 0.000887 0.000022 0.000402
Nucleolus 0.028095 0.026523 0.039007 0.008838 0.020919 0.024983 0.017228 0.015059 0.024514 0.025389 0.033796 0.009807
Nucleus 0.948517* 0.939509* 0.939827* 0.938266* 0.963155* 0.942667* 0.968403* 0.937003* 0.955821* 0.941836* 0.946664* 0.938069*
Peroxisomes 0.000306 0.001586 0.001294 0.000400 0.000367 0.000473 0.000810 0.000684 0.000336 0.000766 0.001178 0.000444
Vacuole 0.001190 0.000599 0.000458 0.001290 0.000532 0.001609 0.000450 0.001396 0.000861 0.001343 0.000456 0.001307
Vacuole Periphery 0.000542 0.000323 0.000133 0.000753 0.000246 0.001303 0.000091 0.001709 0.000394 0.001045 0.000123 0.000902

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.36 -10.43 -7.47 -0.47 -0.40 -3.87 -7.59 -7.33 -4.90 -2.41 -5.03 -13.01 -9.45 -4.00 -1.10
Bud Neck -3.70 -10.29 -12.86 -9.16 -6.20 -5.53 -3.18 -5.92 -4.12 -4.38 -7.53 -10.15 -13.57 -10.14 -7.43
Bud Site -1.27 0.06 -3.82 -0.92 -3.91 -3.10 -0.56 -3.06 -2.00 -2.95 -2.15 -0.77 -4.45 -2.87 -4.33
Cell Periphery 4.08 5.35 3.10 -1.81 -4.89 1.22 2.85 1.69 1.15 -4.23 3.44 5.71 2.85 -0.86 -5.41
Cytoplasm 0.49 3.60 -0.25 -0.67 -4.08 -1.92 3.09 -1.82 0.32 -2.69 -0.73 4.22 -1.60 -0.58 -4.51
Cytoplasmic Foci -0.60 1.54 -1.30 -0.67 -2.50 -1.16 1.78 0.70 1.81 -1.52 -0.95 2.12 -1.56 -1.00 -2.55
Eisosomes 3.00 -0.63 4.16 1.52 8.19 0.99 -2.81 -2.07 -3.18 0.85 3.50 -2.89 3.49 -0.05 8.12
Endoplasmic Reticulum 0.20 9.42 8.90 6.17 -0.17 -4.10 8.80 5.80 8.91 -1.62 -2.67 12.20 10.53 11.54 -0.76
Endosome -0.47 4.56 -1.63 -0.76 -2.81 -2.98 2.34 -1.05 0.51 -1.33 -2.79 4.73 -2.16 0.34 -3.11
Golgi -1.48 1.11 -2.17 -1.28 -2.36 -1.73 0.94 -1.42 1.51 -2.28 -1.90 1.19 -2.29 -0.30 -2.42
Lipid Particles -1.35 1.63 -0.30 1.34 -2.66 -1.89 1.04 -1.02 1.11 -1.62 -1.71 1.71 -1.07 1.62 -3.17
Mitochondria -1.95 -2.53 -6.63 0.63 -5.70 -2.96 -2.01 -3.35 -1.86 -2.80 -3.16 -3.16 -7.56 -1.16 -6.38
Mitotic Spindle -0.58 -0.37 -4.84 -4.66 -4.69 -2.51 1.16 -2.58 -1.99 -2.68 -2.70 0.16 -5.42 -3.79 -5.43
None 0.63 6.16 4.12 1.51 -4.37 -0.42 2.85 -1.33 -1.25 -1.77 1.62 6.75 1.75 0.11 -3.95
Nuclear Periphery 0.03 0.67 -2.85 -2.85 -2.88 -3.46 3.50 -4.61 -0.24 -5.33 -3.45 1.72 -2.96 -2.39 -3.03
Nuclear Periphery Foci 1.74 2.12 -1.16 -2.45 -2.68 -2.45 2.25 -1.02 1.63 -1.34 -2.13 2.57 -1.86 1.10 -2.94
Nucleolus 0.73 -3.76 11.56 7.24 8.83 -2.23 0.35 0.81 2.01 0.34 -0.56 -4.24 10.90 8.98 8.66
Nucleus 1.52 2.83 3.64 1.18 0.79 5.79 -0.39 4.11 1.64 4.05 4.64 3.86 6.36 2.24 2.53
Peroxisomes -1.97 -7.19 -1.24 1.80 6.18 -0.58 -2.52 -1.96 -1.88 0.81 -2.13 -6.67 -1.32 1.53 6.22
Vacuole 2.13 3.00 -0.74 -2.55 -3.20 -2.76 -0.05 -2.56 -0.45 -2.65 -1.62 2.24 -2.30 -0.49 -3.87
Vacuole Periphery 1.23 3.13 -0.58 -1.40 -2.33 -2.19 2.23 -0.94 0.12 -1.09 -1.80 3.63 -1.43 0.61 -2.53
Standard name
Human Ortholog
Description RNA-binding protein; involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes; putative regulatory subunit of type 1 protein phosphatase Glc7p, required for actomyosin ring formation, and for timely dephosphorylation and release of Bnr1p from the division site; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ref2

Ref2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ref2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available