Standard name | |
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Human Ortholog | |
Description | Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance; VPS64 has a paralog, FAR10, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 8 | 0 | 0 | 2 | 1 | 0 | – | 0 | 2 | 0 | 1 | – | – | – | 3 | 3 | 3 | 11 | 11 | 3 | 0 | 0 | 0 |
Bud | 2 | 4 | 3 | 0 | 0 | 0 | 0 | – | 2 | 1 | 1 | 2 | – | – | – | 0 | 2 | 0 | 2 | 4 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | – | – | – | 2 | 2 | 0 | 9 | 16 | 3 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 12 | 21 | 2 | 0 | 6 | 4 | 7 | – | 1 | 2 | 0 | 2 | – | – | – | 35 | 26 | 23 | 7 | 8 | 4 | 0 | 0 | 0 |
Cytoplasm | 239 | 274 | 47 | 20 | 105 | 99 | 134 | – | 80 | 57 | 56 | 80 | – | – | – | 179 | 130 | 102 | 181 | 201 | 33 | 4 | 3 | 3 |
Endoplasmic Reticulum | 37 | 21 | 3 | 0 | 2 | 15 | 10 | – | 7 | 4 | 2 | 9 | – | – | – | 16 | 10 | 3 | 86 | 68 | 11 | 0 | 0 | 0 |
Endosome | 0 | 2 | 0 | 0 | 0 | 1 | 1 | – | 2 | 0 | 0 | 0 | – | – | – | 4 | 5 | 1 | 4 | 2 | 0 | 0 | 0 | 2 |
Golgi | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 2 | 1 | 1 | 2 | 4 | 0 | 0 | 1 | 0 |
Mitochondria | 10 | 4 | 0 | 4 | 1 | 6 | 11 | – | 34 | 15 | 53 | 55 | – | – | – | 7 | 9 | 0 | 26 | 23 | 14 | 2 | 0 | 0 |
Nucleus | 0 | 1 | 1 | 1 | 0 | 0 | 1 | – | 1 | 1 | 1 | 1 | – | – | – | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | – | – | – | 3 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 8 | 0 | 0 | 0 | 1 | 2 | – | 2 | 1 | 0 | 3 | – | – | – | 11 | 10 | 3 | 5 | 6 | 4 | 6 | 4 | 3 |
Unique Cell Count | 284 | 312 | 52 | 21 | 110 | 119 | 157 | 109 | 75 | 91 | 129 | 213 | 159 | 119 | 343 | 353 | 81 | 17 | 18 | 19 | ||||
Labelled Cell Count | 304 | 345 | 56 | 25 | 117 | 127 | 167 | 130 | 84 | 114 | 153 | 265 | 199 | 139 | 343 | 353 | 81 | 17 | 18 | 19 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 6.4 | 5.7 | 5.2 | 6.2 | 5.6 | 5.4 | – | 4.8 | 5.0 | 4.6 | 4.5 | – | – | – | 7.4 | 7.9 | 7.7 | – | – | – |
Std Deviation (1e-4) | 0.5 | 1.1 | 0.8 | 0.5 | 1.0 | 1.0 | 1.3 | – | 1.6 | 0.8 | 1.7 | 0.8 | – | – | – | 1.2 | 1.1 | 1.7 | – | – | – |
Intensity Change (Log2) | – | – | – | -0.12 | 0.13 | -0.01 | -0.06 | – | -0.25 | -0.18 | -0.3 | -0.33 | – | – | – | 0.39 | 0.48 | 0.45 | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 2.3 | 2.3 | 2.6 |
Cytoplasm | 0.7 | 1.3 | -1.2 | -0.9 | – | -2.5 | -2.1 | -3.7 | -3.8 | – | – | – | -1.2 | -1.5 | -0.8 |
Endoplasmic Reticulum | 0 | 0 | 1.3 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 3.4 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5092 | 0.8079 | 0.6125 | 0.4949 | 0.3822 | 0.7349 | 0.8993 | 1.1048 | 1.0537 | 1.2166 | 1.7009 | 1.0145 | 0.4796 | 0.6752 | 0.4739 | 0.7501 | 0.5764 | 0.6435 |
Actin | 0.0262 | 0.0009 | 0.0087 | 0.0021 | 0.0044 | 0.0084 | 0.0281 | 0.0019 | 0.0119 | 0.035 | 0.0332 | 0.0059 | 0.0097 | 0.0046 | 0.0086 | 0.0019 | 0.0007 | 0.0038 |
Bud | 0.005 | 0.0053 | 0.0018 | 0.0149 | 0.0151 | 0.0016 | 0.0015 | 0.0014 | 0.0046 | 0.0008 | 0.0048 | 0.0004 | 0.0013 | 0.0038 | 0.0024 | 0.0006 | 0.0007 | 0.0002 |
Bud Neck | 0.0044 | 0.0008 | 0.0025 | 0.0014 | 0.0012 | 0.0077 | 0.0029 | 0.0011 | 0.0042 | 0.0038 | 0.0137 | 0.0023 | 0.0013 | 0.0003 | 0.0011 | 0.0006 | 0.0008 | 0.0022 |
Bud Periphery | 0.0049 | 0.0023 | 0.0047 | 0.0097 | 0.0069 | 0.0026 | 0.0056 | 0.0053 | 0.0105 | 0.0018 | 0.0097 | 0.0013 | 0.0014 | 0.0025 | 0.0083 | 0.0014 | 0.0011 | 0.0004 |
Bud Site | 0.0068 | 0.0182 | 0.0088 | 0.0011 | 0.0243 | 0.0044 | 0.0096 | 0.0319 | 0.0305 | 0.0107 | 0.0375 | 0.0009 | 0.0062 | 0.0079 | 0.0043 | 0.0006 | 0.001 | 0.0002 |
Cell Periphery | 0.0016 | 0.0013 | 0.0029 | 0.0008 | 0.0007 | 0.0007 | 0.0014 | 0.0022 | 0.0021 | 0.0016 | 0.0009 | 0.0005 | 0.0009 | 0.0008 | 0.0013 | 0.0008 | 0.0003 | 0.0003 |
Cytoplasm | 0.4216 | 0.5743 | 0.4544 | 0.5583 | 0.4972 | 0.5274 | 0.2998 | 0.4679 | 0.4211 | 0.4617 | 0.2752 | 0.4706 | 0.5368 | 0.6696 | 0.6819 | 0.7078 | 0.5834 | 0.6296 |
Cytoplasmic Foci | 0.0297 | 0.02 | 0.0253 | 0.017 | 0.0293 | 0.0366 | 0.0316 | 0.0161 | 0.0192 | 0.0224 | 0.0693 | 0.0113 | 0.0169 | 0.0115 | 0.0109 | 0.0067 | 0.0121 | 0.0171 |
Eisosomes | 0.0006 | 0.0003 | 0.001 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0003 | 0.0004 | 0.0007 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0206 | 0.0086 | 0.013 | 0.0116 | 0.0067 | 0.0137 | 0.0139 | 0.0109 | 0.0163 | 0.0432 | 0.0041 | 0.0101 | 0.0386 | 0.0124 | 0.0247 | 0.0219 | 0.0029 | 0.009 |
Endosome | 0.0354 | 0.0121 | 0.0179 | 0.0173 | 0.0291 | 0.0292 | 0.0325 | 0.0097 | 0.0151 | 0.0271 | 0.0374 | 0.0113 | 0.0196 | 0.008 | 0.009 | 0.0057 | 0.0221 | 0.0066 |
Golgi | 0.008 | 0.0013 | 0.0118 | 0.002 | 0.0106 | 0.004 | 0.0103 | 0.0013 | 0.0033 | 0.0039 | 0.0166 | 0.0027 | 0.0027 | 0.0007 | 0.0018 | 0.0006 | 0.001 | 0.0011 |
Lipid Particles | 0.0218 | 0.0013 | 0.0143 | 0.0006 | 0.0155 | 0.0071 | 0.0171 | 0.0019 | 0.0035 | 0.0095 | 0.0128 | 0.0046 | 0.0084 | 0.001 | 0.0021 | 0.0003 | 0.0006 | 0.0006 |
Mitochondria | 0.0211 | 0.0008 | 0.0094 | 0.0044 | 0.0057 | 0.0049 | 0.0167 | 0.0013 | 0.0038 | 0.0018 | 0.0055 | 0.0076 | 0.0011 | 0.0018 | 0.001 | 0.0006 | 0.0005 | 0.0016 |
None | 0.3507 | 0.342 | 0.3972 | 0.3468 | 0.3409 | 0.3363 | 0.4709 | 0.434 | 0.4351 | 0.3463 | 0.3664 | 0.457 | 0.3402 | 0.2691 | 0.2259 | 0.2442 | 0.3673 | 0.3223 |
Nuclear Periphery | 0.0083 | 0.001 | 0.0022 | 0.0016 | 0.0014 | 0.002 | 0.0135 | 0.0014 | 0.0022 | 0.0033 | 0.0066 | 0.0034 | 0.0036 | 0.0008 | 0.0046 | 0.0014 | 0.0004 | 0.0008 |
Nucleolus | 0.0014 | 0.0006 | 0.001 | 0.0003 | 0.0004 | 0.0005 | 0.0025 | 0.0009 | 0.001 | 0.0025 | 0.0027 | 0.0013 | 0.0005 | 0.0003 | 0.0008 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0029 | 0.002 | 0.0023 | 0.002 | 0.0016 | 0.0021 | 0.0096 | 0.0024 | 0.0034 | 0.0034 | 0.0065 | 0.0022 | 0.0023 | 0.0021 | 0.0024 | 0.0015 | 0.0011 | 0.0009 |
Peroxisomes | 0.0166 | 0.0002 | 0.0109 | 0.0003 | 0.0023 | 0.0032 | 0.0144 | 0.0016 | 0.0035 | 0.0025 | 0.0691 | 0.0016 | 0.001 | 0.0001 | 0.0012 | 0.0001 | 0.0002 | 0.0009 |
Punctate Nuclear | 0.0053 | 0.0028 | 0.0046 | 0.0015 | 0.0017 | 0.0034 | 0.0108 | 0.0014 | 0.0023 | 0.0123 | 0.0125 | 0.0031 | 0.0031 | 0.0005 | 0.004 | 0.0005 | 0.0006 | 0.0009 |
Vacuole | 0.0055 | 0.0036 | 0.0045 | 0.0054 | 0.0037 | 0.0032 | 0.0047 | 0.0048 | 0.0054 | 0.0049 | 0.014 | 0.0015 | 0.0034 | 0.002 | 0.0029 | 0.0024 | 0.0024 | 0.0011 |
Vacuole Periphery | 0.0016 | 0.0003 | 0.0008 | 0.0008 | 0.001 | 0.0007 | 0.0021 | 0.0004 | 0.0007 | 0.0006 | 0.0015 | 0.0004 | 0.0005 | 0.0002 | 0.0005 | 0.0003 | 0.0004 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.9429 | 11.9561 | 6.4523 | 11.9886 | 15.3702 | 11.5444 | 12.3635 | 9.6426 | 12.0994 | 16.5483 |
Translational Efficiency | 1.2826 | 0.9162 | 1.1665 | 0.7387 | 0.6584 | 1.3601 | 0.8852 | 1.2973 | 0.955 | 0.7011 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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