Standard name
Human Ortholog
Description Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.92 0.95 0.88 0.79 – 0.76 0.66 0.64 0.57 0.63 0.98 0.98 0.99 0.97 0.97 0.95 0.83 0.82 0.54 0.82 0.85 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.12 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.12 0.19 0.14 0.42 – 0.46 0.61 0.57 0.6 0.64 0 0 0 0.06 0 0 0.05 0.07 0.27 0.05 0.05 0.05
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 – 2 0 0 0 0 0 0 0 0 0 0 4 3 7 2 1 3
Bud 0 1 2 1 1 5 – 3 7 7 3 7 0 1 0 0 0 0 8 8 1 0 3 4
Bud Neck 0 1 3 0 1 0 – 2 1 3 0 1 0 0 0 1 2 6 1 1 1 2 0 1
Bud Site 0 0 0 0 0 0 – 2 0 3 2 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 11 1 3 0 0 0 – 1 1 1 1 3 4 1 1 4 6 6 3 2 2 1 1 1
Cytoplasm 502 379 180 180 190 216 – 278 146 216 114 152 260 313 314 428 342 407 394 331 108 189 203 191
Endoplasmic Reticulum 0 2 2 1 1 0 – 0 0 1 1 0 3 3 2 15 41 32 1 0 1 1 1 1
Endosome 2 0 0 1 2 1 – 0 0 0 0 0 0 0 0 6 2 6 4 6 5 2 1 4
Golgi 0 4 0 0 0 0 – 0 0 0 0 0 0 1 2 0 0 2 0 3 0 1 2 3
Mitochondria 26 7 23 37 31 114 – 167 135 193 119 155 2 0 0 27 10 16 21 27 55 12 13 10
Nucleus 2 1 1 0 0 0 – 1 2 2 3 2 0 2 0 3 0 1 2 0 0 1 0 0
Nuclear Periphery 0 0 1 0 0 0 – 1 1 0 0 0 0 1 0 0 0 0 2 0 0 1 0 1
Nucleolus 0 0 0 0 0 1 – 1 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0
SpindlePole 0 0 3 0 5 2 – 5 2 8 8 11 0 1 0 0 0 3 7 2 3 1 2 4
Vac/Vac Membrane 3 1 0 0 1 2 – 0 0 0 1 0 0 0 0 7 10 8 7 3 4 6 4 3
Unique Cell Count 514 390 195 190 215 273 366 221 337 199 242 264 318 318 443 353 430 476 402 202 232 240 237
Labelled Cell Count 546 397 219 220 233 341 463 295 434 252 336 269 323 319 493 413 489 476 402 202 232 240 237


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.2 2.9 3.1 3.7 2.9 – 2.9 2.5 2.6 2.6 2.5 4.2 4.4 4.5 4.9 5.0 4.9 4.3 4.4 4.7
Std Deviation (1e-4) 2.1 1.1 0.7 1.8 2.0 1.7 – 1.4 1.5 1.3 0.8 1.2 0.9 1.4 1.5 1.3 1.3 1.5 1.5 1.3 1.8
Intensity Change (Log2) – – – 0.1 0.38 0.0 – 0.02 -0.17 -0.12 -0.16 -0.22 0.56 0.61 0.66 0.78 0.82 0.78 0.57 0.62 0.71


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.178 -0.3414 -0.6412 -1.2247 -0.8656 -0.6768 -1.2079 -1.1322 -1.147 -1.4574 -0.6541 4.6884 0.5803 0.5091 0.6595 0.4161 -0.0577 0.7156
Actin 0.0001 0.0053 0.0003 0.0001 0.0001 0.0005 0.0181 0.0037 0.0214 0.0074 0.0001 0.0003 0.017 0.0002 0.0076 0.0129 0.0032 0.0108
Bud 0 0.0008 0.0007 0.0004 0 0.0016 0.0021 0.0013 0.01 0.0212 0 0.0166 0.0008 0.0007 0.0003 0.0005 0.0001 0.0003
Bud Neck 0 0.0014 0.0001 0 0 0.0039 0.0173 0.0044 0.0006 0.0006 0.0001 0.0008 0.0011 0 0.0005 0.0023 0.0005 0.0003
Bud Periphery 0 0.0005 0.0005 0.0006 0 0.0006 0.0074 0.0014 0.0096 0.0102 0 0.0099 0.0013 0.0006 0.0003 0.0008 0.0001 0.0004
Bud Site 0.0001 0.0041 0.0007 0.0002 0 0.0004 0.0233 0.015 0.0066 0.0072 0.0004 0.0014 0.0014 0.0008 0.0016 0.0043 0.0002 0.0001
Cell Periphery 0 0.0001 0.0001 0 0 0.0001 0.0027 0.0002 0.0009 0.0012 0 0.0008 0.0001 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.0193 0.0316 0.0286 0.0365 0.0158 0.0387 0.0139 0.0191 0.0217 0.0197 0.0024 0.048 0.0151 0.0237 0.0161 0.0344 0.0274 0.0538
Cytoplasmic Foci 0.0044 0.0101 0.0034 0.0026 0.0024 0.0159 0.0181 0.0042 0.014 0.0157 0.221 0.027 0.0123 0.004 0.0046 0.0167 0.0017 0.0023
Eisosomes 0.0002 0.0002 0.0002 0.0001 0 0.0001 0.0008 0.0003 0.0007 0.0002 0.0001 0.0001 0.0002 0.0002 0.0003 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0002 0.0005 0.0004 0.0004 0.0002 0.0002 0.0036 0.0006 0.0009 0.0018 0 0.0006 0.0039 0.0003 0.0023 0.0005 0.0051 0.0005
Endosome 0.0002 0.0005 0.0003 0.0003 0.0002 0.0002 0.0138 0.0016 0.0177 0.0191 0.0638 0.0014 0.0089 0.0002 0.0055 0.0014 0.0122 0.0021
Golgi 0 0.0001 0.0001 0 0 0.0001 0.0067 0.0045 0.0099 0.0185 0.0181 0.0001 0.0026 0 0.0008 0.0018 0.0006 0.0011
Lipid Particles 0.0003 0.0002 0.0002 0.0001 0.0003 0.0006 0.0122 0.0017 0.0027 0.0049 0.0034 0.0005 0.0031 0.0002 0.0005 0.0049 0.0004 0.001
Mitochondria 0.0001 0.0004 0.0003 0.0003 0.0001 0.0008 0.0087 0.0009 0.0133 0.0015 0.0004 0.0007 0.0021 0.0002 0.0013 0.0006 0.0004 0.0009
None 0.9731 0.9365 0.9586 0.9558 0.9801 0.932 0.7887 0.9381 0.8484 0.8572 0.6656 0.8471 0.8731 0.9664 0.9424 0.8877 0.9188 0.9195
Nuclear Periphery 0.0001 0.0006 0.0005 0.0004 0.0001 0.0001 0.0179 0.0003 0.0008 0.0016 0 0.0017 0.0132 0.0005 0.0058 0.0008 0.0149 0.0013
Nucleolus 0.0002 0.0008 0.0005 0.0003 0.0001 0.0007 0.0023 0.0003 0.0009 0.0006 0 0.0017 0.0005 0.0002 0.0006 0.0003 0.0004 0.0001
Nucleus 0.0003 0.0015 0.0016 0.0007 0.0001 0.0008 0.0207 0.0004 0.0048 0.0046 0 0.0152 0.0093 0.0006 0.0045 0.0008 0.0079 0.0025
Peroxisomes 0.0001 0.0003 0.0002 0.0001 0.0001 0.0005 0.0135 0.0005 0.0049 0.0016 0.0238 0.0006 0.0129 0.0001 0.0008 0.0184 0.0001 0.0022
Punctate Nuclear 0.0009 0.004 0.0024 0.0008 0.0003 0.0019 0.0027 0.0011 0.0067 0.0039 0.0005 0.0136 0.0191 0.0008 0.0032 0.0098 0.0045 0.0005
Vacuole 0.0002 0.0003 0.0003 0.0003 0.0002 0.0002 0.0035 0.0003 0.0028 0.0012 0.0002 0.0095 0.0013 0.0002 0.0006 0.0004 0.0009 0.0001
Vacuole Periphery 0 0.0001 0.0001 0.0001 0 0.0001 0.002 0.0001 0.0006 0.0003 0.0001 0.0026 0.0009 0.0001 0.0003 0.0001 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.8332 11.8841 16.1672 13.8346 21.5454 28.7878 22.5299 27.709 23.2833 23.677
Translational Efficiency 2.2178 3.4072 1.2984 2.8027 1.6518 1.3334 1.6961 1.2812 1.6289 1.6613

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1338 1968 1471 235 1899 1966 1556 1399 3237 3934 3027 1634

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 597.97 687.87 789.62 811.22 605.76 681.42 753.52 775.06 602.54 684.65 771.06 780.26
Standard Deviation 72.59 84.21 94.46 106.63 73.29 83.54 85.26 109.99 73.10 83.94 91.64 110.24
Intensity Change Log 2 — 0.202063 0.401085 0.440020 — 0.169798 0.314899 0.355562 — 0.185916 0.358357 0.398136

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000143 0.000364 0.000159 0.003344 0.000115 0.000494 0.000217 0.002278 0.000127 0.000429 0.000189 0.002431
Bud Neck 0.014033 0.054773 0.006565 0.012432 0.024776 0.065137 0.006010 0.012447 0.020335 0.059952 0.006279 0.012445
Bud Site 0.002525 0.017954 0.011973 0.044327 0.003629 0.019762 0.012459 0.061081 0.003172 0.018857 0.012223 0.058672
Cell Periphery 0.000132 0.000119 0.000422 0.000254 0.000129 0.000240 0.000206 0.000202 0.000130 0.000179 0.000311 0.000209
Cytoplasm 0.495202* 0.341010* 0.265454* 0.323335* 0.404615* 0.313560* 0.349467* 0.449624* 0.442058* 0.327292* 0.308640* 0.431461*
Cytoplasmic Foci 0.222171* 0.293351* 0.011552 0.032190 0.256742* 0.278143* 0.012270 0.023632 0.242453* 0.285751* 0.011921 0.024863
Eisosomes 0.000218 0.000148 0.000039 0.000112 0.000220 0.000294 0.000036 0.000046 0.000219 0.000221 0.000037 0.000056
Endoplasmic Reticulum 0.000578 0.000548 0.001770 0.008744 0.000658 0.000537 0.001046 0.001305 0.000625 0.000542 0.001398 0.002375
Endosome 0.009289 0.025770 0.001578 0.028170 0.012233 0.038399 0.002133 0.011525 0.011016 0.032081 0.001863 0.013919
Golgi 0.001787 0.008075 0.000068 0.008319 0.002621 0.009326 0.000110 0.006000 0.002276 0.008700 0.000090 0.006334
Lipid Particles 0.012187 0.007105 0.001189 0.005609 0.016240 0.014522 0.001009 0.001111 0.014565 0.010812 0.001096 0.001758
Mitochondria 0.002920 0.026809 0.000868 0.005844 0.009978 0.035148 0.001423 0.007180 0.007061 0.030977 0.001154 0.006988
Mitotic Spindle 0.000390 0.007425 0.001773 0.016228 0.000468 0.005727 0.012302 0.049861 0.000436 0.006576 0.007185 0.045024
None 0.005749 0.002579 0.008598 0.015585 0.003005 0.002435 0.007238 0.003670 0.004139 0.002507 0.007899 0.005384
Nuclear Periphery 0.000493 0.000409 0.001247 0.003349 0.000755 0.000436 0.001162 0.001426 0.000647 0.000422 0.001203 0.001702
Nuclear Periphery Foci 0.000269 0.000211 0.000366 0.003311 0.000253 0.000258 0.000220 0.000792 0.000259 0.000234 0.000291 0.001155
Nucleolus 0.001599 0.002078 0.000839 0.000735 0.001530 0.002288 0.000589 0.000581 0.001559 0.002183 0.000710 0.000604
Nucleus 0.190720* 0.114406 0.658650* 0.226855* 0.201947* 0.108657 0.561623* 0.258967* 0.197306* 0.111533 0.608774* 0.254348*
Peroxisomes 0.004235 0.031624 0.000481 0.003839 0.009448 0.026970 0.000727 0.002393 0.007293 0.029298 0.000608 0.002601
Vacuole 0.034762 0.062762 0.026046 0.254347* 0.049636 0.074503 0.029446 0.099492 0.043488 0.068629 0.027794 0.121763
Vacuole Periphery 0.000597 0.002481 0.000365 0.003071 0.001004 0.003166 0.000309 0.006388 0.000836 0.002823 0.000336 0.005911

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.89 -1.11 -2.54 -2.02 -2.42 -4.13 -1.96 -1.83 -1.15 -1.64 -5.76 -2.35 -2.12 -1.49 -1.94
Bud Neck -14.67 4.85 1.60 12.59 -1.15 -12.60 11.16 8.45 18.66 -3.08 -18.48 11.84 7.79 24.22 -3.30
Bud Site -11.20 -4.69 -3.04 -0.16 -1.44 -9.81 -3.51 -9.22 -3.83 -6.63 -14.54 -5.70 -9.84 -4.14 -6.88
Cell Periphery 1.51 -1.59 -2.47 -3.32 1.27 -4.20 -0.94 -0.70 3.72 0.66 -3.39 -1.80 -1.20 2.06 1.58
Cytoplasm 17.08 27.05 14.45 5.75 0.24 10.48 10.63 2.12 -7.13 -7.49 18.19 25.06 9.40 -4.28 -10.16
Cytoplasmic Foci -9.21 39.50 32.32 43.48 -3.33 -2.72 47.80 46.10 49.37 -5.42 -7.90 61.76 58.29 69.42 -6.73
Eisosomes 2.56 6.34 3.39 2.83 -9.67 -0.95 19.23 17.63 3.13 -5.91 -0.00 14.04 12.12 4.07 -9.72
Endoplasmic Reticulum 0.19 -10.91 -5.61 -5.65 -3.91 1.50 -9.78 -11.88 -14.02 -2.17 1.11 -14.74 -10.79 -11.49 -3.10
Endosome -10.07 8.46 -0.18 4.37 -2.49 -14.07 14.15 6.04 17.25 -4.97 -17.02 16.33 4.60 18.54 -5.64
Golgi -9.06 4.87 -0.29 3.64 -1.48 -7.65 10.55 0.11 6.09 -3.39 -11.56 10.84 -0.32 7.38 -3.69
Lipid Particles 4.67 10.61 7.07 4.26 -3.21 1.26 14.29 14.06 15.61 -2.69 4.26 17.73 17.03 18.51 -3.32
Mitochondria -11.58 5.46 1.20 11.41 -1.33 -10.11 7.51 4.67 13.55 -3.07 -14.43 8.58 3.93 16.89 -3.52
Mitotic Spindle -5.78 -2.95 -2.64 -1.03 -2.29 -4.97 -5.20 -8.70 -6.84 -4.74 -7.61 -5.71 -9.06 -6.76 -6.55
None 3.68 -1.24 -0.25 -1.23 0.19 0.86 -3.71 -1.68 -3.63 2.76 3.13 -3.44 -0.41 -3.07 2.72
Nuclear Periphery 0.97 -13.16 -6.31 -6.50 -3.96 3.03 -8.73 -10.29 -15.01 -4.76 3.13 -13.60 -13.39 -16.25 -6.70
Nuclear Periphery Foci 1.86 -4.99 -4.67 -4.80 -4.07 -0.11 -1.07 -4.33 -3.92 -4.12 0.48 -3.86 -6.37 -6.52 -4.97
Nucleolus -1.45 3.41 3.27 4.81 0.71 -2.45 5.16 5.20 7.79 0.16 -2.77 5.93 6.75 9.89 1.85
Nucleus 12.83 -55.01 -5.02 -9.76 22.76 16.28 -44.25 -12.68 -24.72 26.65 20.69 -69.17 -14.54 -27.04 38.04
Peroxisomes -15.30 7.32 4.20 17.02 -1.78 -10.58 13.26 11.56 16.92 -1.86 -17.88 15.10 12.02 24.10 -2.47
Vacuole -11.36 -0.98 -17.38 -15.80 -17.20 -7.89 -0.34 -21.22 -16.68 -20.17 -12.45 -0.32 -26.86 -22.35 -26.31
Vacuole Periphery -4.86 3.17 -0.64 2.45 -1.17 -8.72 8.99 -2.25 0.49 -3.23 -8.64 8.81 -2.47 0.43 -3.29
Standard name
Human Ortholog
Description Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Spc19

Spc19


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spc19-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available