Standard name | |
---|---|
Human Ortholog | |
Description | Sterol regulatory element binding protein; induces sterol biosynthetic genes, upon sterol depletion; acts as a sterol sensor, binding ergosterol in sterol rich conditions; relocates from intracellular membranes to perinuclear foci upon sterol depletion; redundant activator of filamentation with ECM22, up-regulating the expression of filamentous growth genes; contains a Zn[2]-Cys[6] binuclear cluster; UPC2 has a paralog, ECM22, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 6 |
Bud | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 5 | 3 | 5 | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 0 | 8 | 16 | 20 |
Bud Neck | 0 | 5 | 6 | 2 | 8 | 3 | 5 | 8 | 0 | 4 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 1 | 7 | 13 | 15 |
Bud Site | 0 | 2 | 0 | 1 | 0 | 2 | 3 | 6 | 11 | 9 | 2 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 31 | 54 | 44 | 22 | 42 | 45 | 83 | 68 | 62 | 41 | 38 | 27 | 50 | 89 | 150 | 93 | 57 | 38 | 14 | 47 | 21 | 44 | 83 | 117 |
Endoplasmic Reticulum | 1 | 2 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 16 | 14 | 11 | 0 | 1 | 0 | 4 | 7 | 8 |
Endosome | 10 | 5 | 2 | 0 | 0 | 2 | 7 | 4 | 0 | 0 | 0 | 0 | 9 | 10 | 6 | 15 | 19 | 12 | 16 | 10 | 4 | 5 | 11 | 5 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 4 | 0 | 0 | 0 | 2 | 1 |
Mitochondria | 47 | 118 | 8 | 6 | 6 | 50 | 88 | 62 | 173 | 132 | 106 | 98 | 21 | 2 | 6 | 21 | 8 | 20 | 3 | 23 | 20 | 26 | 62 | 52 |
Nucleus | 132 | 208 | 53 | 52 | 98 | 53 | 60 | 69 | 64 | 68 | 39 | 61 | 170 | 85 | 66 | 160 | 134 | 110 | 145 | 231 | 77 | 59 | 61 | 53 |
Nuclear Periphery | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 3 | 1 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 19 | 11 | 6 | 6 | 8 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
SpindlePole | 3 | 4 | 6 | 6 | 11 | 6 | 8 | 12 | 6 | 11 | 0 | 14 | 2 | 1 | 1 | 6 | 3 | 10 | 26 | 26 | 17 | 15 | 37 | 30 |
Vac/Vac Membrane | 28 | 31 | 11 | 0 | 13 | 18 | 14 | 13 | 3 | 5 | 2 | 3 | 32 | 64 | 60 | 52 | 42 | 41 | 50 | 114 | 68 | 112 | 176 | 161 |
Unique Cell Count | 226 | 369 | 119 | 84 | 164 | 154 | 223 | 201 | 246 | 197 | 153 | 160 | 267 | 238 | 276 | 317 | 246 | 210 | 300 | 494 | 235 | 304 | 506 | 508 |
Labelled Cell Count | 256 | 432 | 132 | 89 | 184 | 180 | 270 | 248 | 322 | 277 | 198 | 218 | 285 | 252 | 290 | 366 | 279 | 245 | 300 | 494 | 235 | 304 | 506 | 508 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.6 | 3.8 | 4.8 | 4.6 | 5.0 | 3.9 | 4.3 | 4.5 | 3.5 | 3.5 | 3.2 | 3.7 | 4.9 | 5.2 | 5.0 | 6.4 | 6.6 | 6.3 | 4.6 | 4.6 | 5.1 |
Std Deviation (1e-4) | 1.2 | 0.6 | 0.9 | 1.4 | 1.0 | 0.9 | 1.8 | 1.7 | 1.3 | 1.1 | 1.0 | 0.8 | 1.2 | 1.7 | 1.7 | 1.6 | 1.8 | 1.6 | 1.5 | 1.6 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.06 | -0.31 | -0.14 | -0.09 | -0.44 | -0.46 | -0.58 | -0.35 | 0.03 | 0.12 | 0.08 | 0.43 | 0.48 | 0.4 | -0.04 | -0.04 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 1.9 | 2.0 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.5934 | 2.2907 | 1.8079 | 1.6655 | 1.0473 | 1.8265 | 0.5992 | 0.4963 | 0.3538 | 0.6031 | 0.0247 | 0.2948 | -0.0689 | 0.0729 | 0.0568 | 0.4721 | 0.5369 | 0.2629 |
Actin | 0.0383 | 0.0002 | 0.0003 | 0.0015 | 0.0006 | 0.0024 | 0.0137 | 0.0002 | 0.0017 | 0.0014 | 0.0039 | 0.0015 | 0.0125 | 0.0002 | 0.0001 | 0.0113 | 0.0052 | 0.0035 |
Bud | 0.0004 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0034 | 0.0061 | 0.0003 | 0.0002 | 0.0005 | 0.0002 | 0.0004 | 0.0015 | 0.0001 | 0.0002 | 0.0002 | 0.0003 |
Bud Neck | 0.0163 | 0.0009 | 0.0015 | 0.0053 | 0.0024 | 0.0088 | 0.0039 | 0.0006 | 0.0077 | 0.0019 | 0.002 | 0.0039 | 0.0039 | 0.0008 | 0.0005 | 0.0034 | 0.0038 | 0.004 |
Bud Periphery | 0.0008 | 0.0001 | 0.0001 | 0.002 | 0.0001 | 0.0003 | 0.002 | 0.0006 | 0.0007 | 0.0005 | 0.0008 | 0.0002 | 0.0003 | 0.0007 | 0.0001 | 0.0002 | 0.0003 | 0.0004 |
Bud Site | 0.0095 | 0.0024 | 0.0006 | 0.0045 | 0.0002 | 0.0004 | 0.0057 | 0.0029 | 0.0037 | 0.0004 | 0.0028 | 0.0002 | 0.0022 | 0.0039 | 0.0003 | 0.0003 | 0.0003 | 0.0002 |
Cell Periphery | 0.0005 | 0.0001 | 0.0001 | 0.0021 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0302 | 0.0504 | 0.021 | 0.0329 | 0.0557 | 0.0305 | 0.0228 | 0.0353 | 0.0159 | 0.0108 | 0.0346 | 0.0117 | 0.0281 | 0.0348 | 0.0232 | 0.0149 | 0.0179 | 0.0146 |
Cytoplasmic Foci | 0.0156 | 0.0017 | 0.0033 | 0.0057 | 0.0021 | 0.0041 | 0.0185 | 0.0009 | 0.0062 | 0.0167 | 0.0187 | 0.0019 | 0.0108 | 0.0022 | 0.0025 | 0.0104 | 0.0121 | 0.011 |
Eisosomes | 0.0007 | 0.0001 | 0.0002 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0026 | 0.0025 | 0.0018 | 0.0086 | 0.0075 | 0.0017 | 0.0041 | 0.0018 | 0.0021 | 0.0012 | 0.005 | 0.0007 | 0.0021 | 0.0012 | 0.0007 | 0.0024 | 0.0023 | 0.001 |
Endosome | 0.0094 | 0.0017 | 0.0051 | 0.0169 | 0.0066 | 0.0036 | 0.0353 | 0.0019 | 0.0145 | 0.0413 | 0.0339 | 0.0017 | 0.0086 | 0.001 | 0.0009 | 0.0047 | 0.0038 | 0.0029 |
Golgi | 0.0056 | 0.0001 | 0.0008 | 0.0026 | 0.0002 | 0.0004 | 0.0103 | 0 | 0.0047 | 0.0032 | 0.0096 | 0.0004 | 0.0014 | 0 | 0 | 0.001 | 0.0005 | 0.0006 |
Lipid Particles | 0.0133 | 0.0003 | 0.0058 | 0.0058 | 0.0015 | 0.0021 | 0.0201 | 0.0001 | 0.0059 | 0.0082 | 0.0161 | 0.0017 | 0.0026 | 0.0001 | 0.0001 | 0.001 | 0.0009 | 0.0015 |
Mitochondria | 0.0038 | 0.0008 | 0.001 | 0.0299 | 0.033 | 0.0012 | 0.007 | 0.0006 | 0.0026 | 0.0027 | 0.0049 | 0.0005 | 0.0009 | 0.0006 | 0.0007 | 0.001 | 0.0021 | 0.002 |
None | 0.1134 | 0.21 | 0.1401 | 0.1342 | 0.1572 | 0.0968 | 0.0205 | 0.0217 | 0.0145 | 0.0094 | 0.0589 | 0.023 | 0.0446 | 0.1209 | 0.0474 | 0.0588 | 0.0592 | 0.0295 |
Nuclear Periphery | 0.0137 | 0.0292 | 0.025 | 0.0212 | 0.0368 | 0.014 | 0.0361 | 0.0189 | 0.0168 | 0.0211 | 0.0308 | 0.0112 | 0.0163 | 0.0141 | 0.0186 | 0.0322 | 0.0433 | 0.0124 |
Nucleolus | 0.0242 | 0.018 | 0.0379 | 0.0845 | 0.034 | 0.0356 | 0.0147 | 0.0153 | 0.0178 | 0.0189 | 0.0177 | 0.0255 | 0.0089 | 0.0097 | 0.0124 | 0.0076 | 0.0125 | 0.0205 |
Nucleus | 0.6175 | 0.6589 | 0.6694 | 0.5396 | 0.5917 | 0.7142 | 0.7183 | 0.8456 | 0.8273 | 0.7706 | 0.6954 | 0.8619 | 0.6969 | 0.6404 | 0.6777 | 0.6832 | 0.6032 | 0.6602 |
Peroxisomes | 0.01 | 0.0001 | 0.0018 | 0.0015 | 0.0007 | 0.0004 | 0.0058 | 0 | 0.0029 | 0.0038 | 0.0114 | 0.0015 | 0.0018 | 0.0001 | 0 | 0.0003 | 0.0003 | 0.0004 |
Punctate Nuclear | 0.0712 | 0.0197 | 0.0812 | 0.085 | 0.0656 | 0.0814 | 0.0479 | 0.0448 | 0.0506 | 0.0758 | 0.049 | 0.0512 | 0.1559 | 0.1668 | 0.2139 | 0.1658 | 0.2292 | 0.2338 |
Vacuole | 0.0023 | 0.0023 | 0.0022 | 0.0136 | 0.0034 | 0.0016 | 0.0061 | 0.0021 | 0.0029 | 0.0075 | 0.0026 | 0.001 | 0.0013 | 0.0008 | 0.0004 | 0.0006 | 0.0016 | 0.0006 |
Vacuole Periphery | 0.0006 | 0.0004 | 0.0007 | 0.0015 | 0.0005 | 0.0004 | 0.0031 | 0.0004 | 0.0009 | 0.0043 | 0.0013 | 0.0003 | 0.0005 | 0.0002 | 0.0003 | 0.0005 | 0.001 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.0783 | 11.871 | 6.4064 | 10.3759 | 14.087 | 6.8372 | 9.9114 | 12.1516 | 11.9172 | 13.9489 |
Translational Efficiency | 0.8425 | 0.8669 | 1.1142 | 0.6826 | 0.6026 | 1.4253 | 0.7324 | 0.609 | 0.5852 | 0.66 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1784 | 1328 | 341 | 1679 | 259 | 1339 | 1988 | 300 | 2043 | 2667 | 2329 | 1979 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 601.35 | 728.11 | 897.27 | 827.86 | 670.38 | 742.67 | 806.41 | 823.08 | 610.10 | 735.42 | 819.71 | 827.14 |
Standard Deviation | 65.89 | 95.68 | 106.85 | 118.55 | 71.05 | 92.35 | 98.36 | 102.55 | 70.42 | 94.30 | 104.69 | 116.28 |
Intensity Change Log 2 | — | 0.275952 | 0.577337 | 0.461182 | — | 0.147742 | 0.266534 | 0.296054 | — | 0.209789 | 0.421862 | 0.376495 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000053 | 0.000394 | 0.001289 | 0.001830 | 0.000239 | 0.000254 | 0.000315 | 0.000722 | 0.000076 | 0.000324 | 0.000458 | 0.001662 |
Bud Neck | 0.003151 | 0.008017 | 0.009608 | 0.026685 | 0.001529 | 0.008719 | 0.009483 | 0.006776 | 0.002946 | 0.008369 | 0.009501 | 0.023667 |
Bud Site | 0.001370 | 0.001858 | 0.007449 | 0.031179 | 0.000328 | 0.003881 | 0.002898 | 0.022866 | 0.001238 | 0.002874 | 0.003564 | 0.029918 |
Cell Periphery | 0.000145 | 0.000101 | 0.000093 | 0.000299 | 0.000118 | 0.000094 | 0.000114 | 0.000226 | 0.000141 | 0.000098 | 0.000111 | 0.000288 |
Cytoplasm | 0.179186* | 0.119914* | 0.004307 | 0.112631* | 0.073518 | 0.102679* | 0.032327 | 0.122850* | 0.165790* | 0.111261* | 0.028224 | 0.114180* |
Cytoplasmic Foci | 0.025559 | 0.028844 | 0.000197 | 0.005666 | 0.033498 | 0.026782 | 0.000754 | 0.000882 | 0.026565 | 0.027809 | 0.000672 | 0.004940 |
Eisosomes | 0.000032 | 0.000043 | 0.000052 | 0.000041 | 0.000085 | 0.000043 | 0.000021 | 0.000041 | 0.000039 | 0.000043 | 0.000026 | 0.000041 |
Endoplasmic Reticulum | 0.000582 | 0.003773 | 0.012724 | 0.008483 | 0.002029 | 0.002555 | 0.003781 | 0.006496 | 0.000765 | 0.003161 | 0.005090 | 0.008182 |
Endosome | 0.001394 | 0.002330 | 0.000562 | 0.009020 | 0.001939 | 0.003070 | 0.000736 | 0.001038 | 0.001463 | 0.002701 | 0.000710 | 0.007810 |
Golgi | 0.000249 | 0.001640 | 0.000095 | 0.008085 | 0.000458 | 0.001031 | 0.000058 | 0.000089 | 0.000275 | 0.001334 | 0.000063 | 0.006873 |
Lipid Particles | 0.004093 | 0.005660 | 0.000611 | 0.000956 | 0.007781 | 0.005861 | 0.000502 | 0.000890 | 0.004560 | 0.005761 | 0.000518 | 0.000946 |
Mitochondria | 0.003600 | 0.010166 | 0.001137 | 0.010768 | 0.005174 | 0.009469 | 0.001622 | 0.001747 | 0.003800 | 0.009816 | 0.001551 | 0.009400 |
Mitotic Spindle | 0.002335 | 0.012136 | 0.000421 | 0.059861 | 0.000101 | 0.016553 | 0.005105 | 0.018625 | 0.002052 | 0.014354 | 0.004419 | 0.053610 |
None | 0.016670 | 0.011476 | 0.002195 | 0.009783 | 0.011141 | 0.007673 | 0.005629 | 0.024829 | 0.015969 | 0.009567 | 0.005126 | 0.012064 |
Nuclear Periphery | 0.001505 | 0.004609 | 0.001233 | 0.006524 | 0.001732 | 0.004364 | 0.001624 | 0.002648 | 0.001534 | 0.004486 | 0.001566 | 0.005937 |
Nuclear Periphery Foci | 0.000349 | 0.001861 | 0.000876 | 0.002244 | 0.002074 | 0.001425 | 0.000410 | 0.001144 | 0.000567 | 0.001642 | 0.000478 | 0.002077 |
Nucleolus | 0.008154 | 0.012717 | 0.011829 | 0.002901 | 0.016210 | 0.012418 | 0.002724 | 0.001809 | 0.009175 | 0.012567 | 0.004057 | 0.002736 |
Nucleus | 0.747137* | 0.762108* | 0.937394* | 0.655337* | 0.832239* | 0.779567* | 0.925986* | 0.758936* | 0.757926* | 0.770873* | 0.927656* | 0.671042* |
Peroxisomes | 0.001638 | 0.004826 | 0.000369 | 0.001114 | 0.004557 | 0.004719 | 0.000244 | 0.000160 | 0.002008 | 0.004772 | 0.000262 | 0.000969 |
Vacuole | 0.002395 | 0.006725 | 0.007365 | 0.040787 | 0.004937 | 0.008109 | 0.005244 | 0.026982 | 0.002717 | 0.007420 | 0.005554 | 0.038694 |
Vacuole Periphery | 0.000405 | 0.000802 | 0.000194 | 0.005808 | 0.000313 | 0.000733 | 0.000425 | 0.000245 | 0.000393 | 0.000767 | 0.000391 | 0.004965 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.12 | -13.87 | -6.39 | -3.73 | 1.20 | -0.35 | -2.54 | -3.81 | -3.73 | -3.18 | -7.04 | -12.87 | -7.19 | -5.05 | -3.82 |
Bud Neck | -6.23 | -5.95 | -14.59 | -9.56 | -5.66 | -6.50 | -8.08 | -8.03 | -1.22 | 0.12 | -8.01 | -8.57 | -15.42 | -9.91 | -7.72 |
Bud Site | -1.49 | -1.56 | -8.65 | -8.39 | -3.35 | -3.95 | -5.39 | -3.43 | -2.45 | -2.60 | -2.88 | -3.37 | -9.27 | -8.10 | -7.38 |
Cell Periphery | 1.17 | 1.27 | -1.11 | -3.64 | -3.52 | 2.41 | 1.43 | -1.47 | -3.89 | -2.92 | 1.48 | 1.19 | -1.21 | -4.39 | -3.74 |
Cytoplasm | 8.74 | 34.10 | 13.30 | 3.97 | -22.83 | -3.56 | 6.07 | -0.74 | 2.35 | -6.24 | 9.87 | 28.78 | 12.75 | 3.76 | -17.20 |
Cytoplasmic Foci | -0.93 | 15.07 | 13.46 | 11.97 | -7.81 | 1.66 | 7.49 | 7.36 | 12.31 | -1.49 | -0.24 | 16.21 | 15.22 | 16.79 | -4.39 |
Eisosomes | -5.05 | -5.23 | -7.45 | -1.61 | 0.79 | 4.98 | 6.91 | 1.22 | -5.55 | -8.80 | -2.13 | 3.61 | -5.78 | -3.87 | -10.21 |
Endoplasmic Reticulum | -11.22 | -14.86 | -22.98 | -12.15 | 2.53 | -1.53 | -8.56 | -9.06 | -8.67 | -6.36 | -12.84 | -20.39 | -24.73 | -16.84 | -9.35 |
Endosome | -2.71 | 1.68 | -4.02 | -2.19 | -5.96 | -0.58 | 1.88 | 0.19 | 1.47 | -4.74 | -3.19 | 2.14 | -3.88 | -1.55 | -6.34 |
Golgi | -2.40 | 1.60 | -2.78 | -0.99 | -2.94 | -1.77 | 2.80 | 2.19 | 3.71 | -4.74 | -3.35 | 3.05 | -2.70 | -1.16 | -3.03 |
Lipid Particles | -2.26 | 8.40 | 7.22 | 8.37 | -4.84 | 1.79 | 7.49 | 6.38 | 6.16 | -4.37 | -2.04 | 10.62 | 8.91 | 10.49 | -7.65 |
Mitochondria | -4.24 | 3.22 | -2.30 | 2.69 | -6.59 | -1.29 | 1.03 | 1.05 | 5.58 | 0.22 | -4.91 | 2.64 | -1.58 | 3.93 | -5.66 |
Mitotic Spindle | -4.79 | 0.16 | -9.76 | -5.51 | -9.54 | -6.09 | -5.31 | -4.68 | -2.18 | -3.78 | -6.87 | -2.80 | -10.76 | -5.66 | -9.36 |
None | 3.01 | 11.07 | 7.20 | 3.89 | -6.43 | 2.11 | 3.92 | 0.71 | -0.95 | -2.32 | 4.89 | 9.75 | 7.07 | 2.65 | -3.73 |
Nuclear Periphery | -8.59 | -0.74 | -11.41 | -4.58 | -11.22 | -4.78 | -1.99 | -5.12 | -1.23 | -4.45 | -9.76 | -4.57 | -12.30 | -4.92 | -10.26 |
Nuclear Periphery Foci | -5.08 | -2.08 | -5.68 | 1.25 | -1.18 | 0.29 | 1.65 | -0.71 | -1.13 | -2.54 | -4.47 | -1.14 | -4.99 | 0.47 | -3.44 |
Nucleolus | -3.93 | -2.05 | 6.59 | 9.44 | 4.64 | 0.85 | 5.11 | 5.65 | 9.26 | 2.58 | -3.80 | 4.84 | 8.19 | 13.10 | 4.76 |
Nucleus | -0.64 | -19.50 | 4.08 | 4.37 | 22.10 | 3.43 | -7.15 | 5.55 | 3.62 | 12.12 | -0.90 | -24.47 | 5.56 | 6.59 | 28.33 |
Peroxisomes | -4.94 | 4.13 | 3.68 | 7.77 | -0.80 | 0.09 | 3.62 | 3.66 | 6.15 | 0.41 | -4.84 | 5.45 | 5.03 | 9.30 | -1.08 |
Vacuole | -6.47 | -4.58 | -19.69 | -17.62 | -14.11 | -2.96 | -3.74 | -9.61 | -9.26 | -9.20 | -7.69 | -7.77 | -21.84 | -19.92 | -19.30 |
Vacuole Periphery | -1.64 | 1.41 | -3.39 | -2.52 | -3.94 | -3.38 | -0.03 | 0.92 | 4.36 | 0.43 | -2.29 | 0.40 | -3.35 | -2.49 | -3.46 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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