Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondria-associated F-box protein; involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 4 | 1 | 8 | 8 | 26 | 8 | 19 | 1 | 10 | 5 | 13 |
Bud | 10 | 9 | 9 | 2 | 11 | 15 | 7 | 13 | 12 | 18 | 16 | 13 | 22 | 14 | 9 | 23 | 26 | 18 | 4 | 12 | 2 | 5 | 4 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 1 | 3 | 3 | 10 |
Bud Site | 1 | 3 | 0 | 3 | 0 | 2 | 3 | 6 | 6 | 5 | 3 | 3 | 2 | 2 | 1 | 10 | 8 | 10 | – | – | – | – | – | – |
Cell Periphery | 1 | 8 | 0 | 2 | 0 | 1 | 1 | 1 | 2 | 0 | 2 | 2 | 7 | 7 | 2 | 10 | 8 | 12 | 0 | 0 | 0 | 1 | 2 | 2 |
Cytoplasm | 10 | 13 | 6 | 16 | 24 | 37 | 32 | 73 | 29 | 34 | 19 | 28 | 32 | 34 | 46 | 40 | 24 | 19 | 1 | 4 | 0 | 48 | 110 | 101 |
Endoplasmic Reticulum | 2 | 7 | 0 | 1 | 1 | 2 | 3 | 1 | 0 | 1 | 0 | 0 | 1 | 4 | 8 | 5 | 10 | 4 | 0 | 2 | 0 | 1 | 5 | 3 |
Endosome | 17 | 26 | 1 | 0 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 51 | 77 | 77 | 63 | 46 | 26 | 28 | 43 | 13 | 25 | 13 | 10 |
Golgi | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 8 | 10 | 15 | 37 | 33 | 9 | 14 | 1 | 4 | 0 | 1 |
Mitochondria | 126 | 121 | 65 | 51 | 63 | 105 | 113 | 188 | 118 | 102 | 121 | 99 | 152 | 125 | 128 | 27 | 39 | 31 | 75 | 81 | 40 | 78 | 61 | 51 |
Nucleus | 2 | 0 | 1 | 0 | 5 | 0 | 3 | 4 | 8 | 3 | 1 | 5 | 1 | 0 | 0 | 4 | 8 | 7 | 0 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 4 | 1 | 0 | 1 | 3 | 1 | 0 | 1 | 3 | 2 | 2 | 1 | 0 | 1 | 1 | 0 | 0 |
Nucleolus | 14 | 11 | 4 | 1 | 3 | 2 | 2 | 5 | 3 | 2 | 0 | 0 | 11 | 13 | 5 | 18 | 5 | 19 | 2 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 12 | 6 | 2 | 0 | 1 | 2 | 1 | 6 | 1 | 1 | 0 | 2 | 32 | 55 | 37 | 80 | 51 | 38 | 15 | 10 | 5 | 7 | 0 | 0 |
SpindlePole | 9 | 5 | 2 | 2 | 4 | 6 | 4 | 26 | 3 | 6 | 9 | 7 | 8 | 19 | 10 | 65 | 53 | 42 | 9 | 6 | 3 | 12 | 9 | 7 |
Vac/Vac Membrane | 10 | 25 | 0 | 4 | 1 | 5 | 2 | 11 | 1 | 1 | 0 | 3 | 32 | 34 | 35 | 33 | 33 | 14 | 3 | 19 | 1 | 6 | 7 | 14 |
Unique Cell Count | 164 | 190 | 75 | 64 | 93 | 151 | 147 | 271 | 148 | 138 | 144 | 133 | 290 | 294 | 295 | 289 | 260 | 222 | 169 | 226 | 80 | 216 | 237 | 235 |
Labelled Cell Count | 217 | 242 | 91 | 83 | 115 | 181 | 175 | 342 | 185 | 174 | 172 | 165 | 368 | 396 | 370 | 404 | 358 | 302 | 169 | 226 | 80 | 216 | 237 | 235 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.7 | 6.3 | 4.4 | 4.4 | 4.6 | 4.4 | 3.4 | 4.4 | 4.0 | 3.8 | 3.9 | 3.9 | 6.3 | 6.3 | 6.0 | 7.1 | 8.3 | 8.5 | 5.0 | 5.1 | 5.9 |
Std Deviation (1e-4) | 0.9 | 1.1 | 0.9 | 1.0 | 1.3 | 1.3 | 1.0 | 1.7 | 1.3 | 1.5 | 1.5 | 1.9 | 1.2 | 1.4 | 1.5 | 1.6 | 1.6 | 1.7 | 1.3 | 1.9 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.0 | 0.05 | -0.01 | -0.39 | -0.02 | -0.15 | -0.21 | -0.18 | -0.18 | 0.5 | 0.51 | 0.44 | 0.68 | 0.9 | 0.93 | 0.17 | 0.19 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.2 | 0 | 0 | -1.1 | -0.5 | -1.0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.7 | 3.0 | 3.0 | 2.6 | 3.5 | 2.2 | 3.0 | 1.1 | 2.4 | 0.8 | 0.9 | 1.7 | 1.4 | 0.3 | 0.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 4.7 | 4.7 | 4.1 | 3.6 | 2.7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 3.5 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.4 | -6.8 | -6.7 | -13.7 | -11.8 | -11.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 3.4 | 2.5 | 4.6 | 3.5 | 3.2 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 3.6 | 3.4 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 3.1 | 3.1 | 3.1 | 3.2 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9028 | 3.6428 | 3.7706 | 3.4183 | 3.7035 | 3.5045 | 2.7347 | 3.2349 | 3.1038 | 3.5237 | 2.0903 | 2.69 | 4.4837 | 4.3441 | 4.0577 | 4.5461 | 4.4154 | 4.0261 |
Actin | 0.0661 | 0.0732 | 0.054 | 0.0324 | 0.1089 | 0.0449 | 0.0865 | 0.0664 | 0.0662 | 0.0349 | 0.0191 | 0.0383 | 0.0944 | 0.0864 | 0.0539 | 0.1066 | 0.0871 | 0.0667 |
Bud | 0.0017 | 0.0033 | 0.0295 | 0.0026 | 0.0205 | 0.0016 | 0.0095 | 0.005 | 0.0252 | 0.0432 | 0.0173 | 0.0017 | 0.0019 | 0.0185 | 0.0227 | 0.0259 | 0.0121 | 0.0059 |
Bud Neck | 0.0213 | 0.0046 | 0.0061 | 0.0006 | 0.001 | 0.0092 | 0.0023 | 0.0015 | 0.0015 | 0.0009 | 0.0002 | 0.0014 | 0.0033 | 0.001 | 0.0012 | 0.0097 | 0.001 | 0.0039 |
Bud Periphery | 0.0037 | 0.006 | 0.0549 | 0.0046 | 0.1248 | 0.0033 | 0.0175 | 0.0085 | 0.0789 | 0.1781 | 0.1234 | 0.0048 | 0.0027 | 0.0278 | 0.0672 | 0.0932 | 0.0495 | 0.0188 |
Bud Site | 0.013 | 0.0571 | 0.0687 | 0.0039 | 0.0049 | 0.0031 | 0.0243 | 0.0675 | 0.0685 | 0.0636 | 0.0012 | 0.002 | 0.0241 | 0.0265 | 0.0411 | 0.0128 | 0.0093 | 0.0084 |
Cell Periphery | 0.0021 | 0.0039 | 0.0114 | 0.0004 | 0.0016 | 0.0005 | 0.0014 | 0.0021 | 0.0017 | 0.0035 | 0.0009 | 0.0006 | 0.0021 | 0.0009 | 0.0037 | 0.002 | 0.0024 | 0.0017 |
Cytoplasm | 0.0448 | 0.0124 | 0.0322 | 0.001 | 0.0069 | 0.0027 | 0.0318 | 0.0121 | 0.0104 | 0.0026 | 0.0005 | 0.0028 | 0.0225 | 0.0081 | 0.0113 | 0.0035 | 0.0379 | 0.0087 |
Cytoplasmic Foci | 0.1522 | 0.1325 | 0.1104 | 0.0383 | 0.0569 | 0.093 | 0.1972 | 0.127 | 0.1391 | 0.0787 | 0.0506 | 0.1305 | 0.1772 | 0.1268 | 0.1078 | 0.1128 | 0.1086 | 0.1414 |
Eisosomes | 0.0015 | 0.0025 | 0.0008 | 0.0005 | 0.0008 | 0.0004 | 0.0011 | 0.0017 | 0.0009 | 0.0007 | 0.0007 | 0.0004 | 0.0012 | 0.0007 | 0.0011 | 0.0005 | 0.001 | 0.0007 |
Endoplasmic Reticulum | 0.0245 | 0.0007 | 0.0079 | 0.0002 | 0.0017 | 0.0005 | 0.005 | 0.0023 | 0.0012 | 0.0005 | 0.0003 | 0.0017 | 0.0095 | 0.0026 | 0.0032 | 0.0004 | 0.0042 | 0.0017 |
Endosome | 0.1312 | 0.1299 | 0.139 | 0.0419 | 0.1278 | 0.1186 | 0.1556 | 0.1782 | 0.0874 | 0.0553 | 0.0434 | 0.1649 | 0.1733 | 0.1265 | 0.1251 | 0.1173 | 0.0895 | 0.1276 |
Golgi | 0.0863 | 0.1061 | 0.071 | 0.0762 | 0.0321 | 0.1252 | 0.0551 | 0.0451 | 0.0454 | 0.0525 | 0.0576 | 0.1077 | 0.0896 | 0.1098 | 0.0817 | 0.122 | 0.1125 | 0.1425 |
Lipid Particles | 0.0833 | 0.0801 | 0.0223 | 0.0359 | 0.0404 | 0.0767 | 0.0537 | 0.0312 | 0.0115 | 0.0479 | 0.0957 | 0.0578 | 0.0648 | 0.0517 | 0.0411 | 0.0175 | 0.0105 | 0.0607 |
Mitochondria | 0.1359 | 0.2306 | 0.2536 | 0.6726 | 0.3489 | 0.4239 | 0.1744 | 0.2696 | 0.313 | 0.3045 | 0.4064 | 0.3447 | 0.2092 | 0.2903 | 0.2916 | 0.2547 | 0.3093 | 0.2445 |
None | 0.0088 | 0.0132 | 0.0089 | 0.0005 | 0.001 | 0.0006 | 0.0188 | 0.0457 | 0.0102 | 0.0057 | 0.0017 | 0.0008 | 0.0183 | 0.0285 | 0.0145 | 0.0015 | 0.0378 | 0.0009 |
Nuclear Periphery | 0.0351 | 0.0005 | 0.0175 | 0.0001 | 0.0009 | 0.0002 | 0.016 | 0.0011 | 0.0005 | 0.0003 | 0.0001 | 0.0004 | 0.0054 | 0.0011 | 0.0011 | 0.0002 | 0.0014 | 0.0003 |
Nucleolus | 0.0014 | 0.0023 | 0.0014 | 0.0004 | 0.0006 | 0.0004 | 0.0242 | 0.0012 | 0.0016 | 0.002 | 0.0005 | 0.0004 | 0.0007 | 0.0006 | 0.0009 | 0.0004 | 0.0004 | 0.0003 |
Nucleus | 0.0083 | 0.0007 | 0.0081 | 0.0003 | 0.0006 | 0.0003 | 0.0327 | 0.0008 | 0.0012 | 0.0009 | 0.0002 | 0.0003 | 0.0015 | 0.0007 | 0.0007 | 0.0003 | 0.0004 | 0.0003 |
Peroxisomes | 0.1528 | 0.1168 | 0.0726 | 0.0823 | 0.1079 | 0.0884 | 0.0476 | 0.1054 | 0.1144 | 0.1147 | 0.1757 | 0.1276 | 0.0764 | 0.0836 | 0.1136 | 0.1131 | 0.1173 | 0.1576 |
Punctate Nuclear | 0.0136 | 0.0007 | 0.0012 | 0.0002 | 0.0005 | 0.0005 | 0.0272 | 0.0009 | 0.0033 | 0.0012 | 0.002 | 0.0007 | 0.0106 | 0.0006 | 0.0029 | 0.0005 | 0.001 | 0.0012 |
Vacuole | 0.0073 | 0.0191 | 0.0232 | 0.0026 | 0.0075 | 0.0034 | 0.0129 | 0.0203 | 0.0144 | 0.0057 | 0.0013 | 0.0063 | 0.0082 | 0.0051 | 0.0104 | 0.0033 | 0.0045 | 0.0035 |
Vacuole Periphery | 0.0052 | 0.0036 | 0.0052 | 0.0024 | 0.0039 | 0.0029 | 0.0051 | 0.0062 | 0.0034 | 0.0027 | 0.0014 | 0.0043 | 0.003 | 0.0022 | 0.0032 | 0.0019 | 0.0024 | 0.0027 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.6782 | 15.5224 | 10.4712 | 10.7782 | 13.8149 | 17.3544 | 17.8348 | 20.4634 | 12.9438 | 21.1487 |
Translational Efficiency | 0.8081 | 1.4157 | 1.069 | 0.9972 | 1.309 | 1.0578 | 1.1149 | 0.9164 | 1.0577 | 1.1414 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2066 | 1354 | 1852 | 1685 | 1836 | 847 | 233 | 186 | 3902 | 2201 | 2085 | 1871 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 779.62 | 694.67 | 991.45 | 1051.44 | 775.84 | 796.08 | 1200.89 | 1132.78 | 777.84 | 733.70 | 1014.86 | 1059.53 |
Standard Deviation | 96.14 | 104.10 | 123.63 | 136.89 | 100.53 | 106.30 | 137.61 | 137.73 | 98.25 | 115.97 | 141.59 | 139.12 |
Intensity Change Log 2 | — | -0.166443 | 0.346769 | 0.431524 | — | 0.037154 | 0.630273 | 0.546037 | — | -0.061303 | 0.495130 | 0.489777 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003320 | 0.005229 | 0.003466 | 0.029714 | 0.001556 | 0.002660 | 0.019820 | 0.023337 | 0.002490 | 0.004240 | 0.005294 | 0.029080 |
Bud Neck | 0.012208 | 0.019320 | 0.017474 | 0.011048 | 0.025630 | 0.009011 | 0.021310 | 0.012355 | 0.018523 | 0.015353 | 0.017903 | 0.011178 |
Bud Site | 0.062632 | 0.074534 | 0.118430* | 0.377928* | 0.031879 | 0.044939 | 0.281897* | 0.275007* | 0.048162 | 0.063145 | 0.136698* | 0.367697* |
Cell Periphery | 0.001213 | 0.001408 | 0.000277 | 0.000684 | 0.000975 | 0.000401 | 0.000417 | 0.000250 | 0.001101 | 0.001020 | 0.000293 | 0.000641 |
Cytoplasm | 0.012655 | 0.015590 | 0.037176 | 0.108173* | 0.015337 | 0.013837 | 0.038358 | 0.068376 | 0.013917 | 0.014916 | 0.037308 | 0.104217* |
Cytoplasmic Foci | 0.054735 | 0.072786 | 0.092968 | 0.055920 | 0.077374 | 0.072439 | 0.102998 | 0.131355 | 0.065387 | 0.072653 | 0.094089 | 0.063419 |
Eisosomes | 0.000110 | 0.000156 | 0.000071 | 0.000105 | 0.000134 | 0.000094 | 0.000106 | 0.000071 | 0.000121 | 0.000133 | 0.000075 | 0.000101 |
Endoplasmic Reticulum | 0.000518 | 0.000720 | 0.000947 | 0.000685 | 0.000840 | 0.000355 | 0.001760 | 0.000825 | 0.000669 | 0.000580 | 0.001038 | 0.000699 |
Endosome | 0.144242 | 0.139313 | 0.168549 | 0.033624 | 0.152962 | 0.128946 | 0.093662 | 0.093085 | 0.148345 | 0.135323 | 0.160180 | 0.039535 |
Golgi | 0.097359 | 0.122379* | 0.070509 | 0.055469 | 0.113286* | 0.171045* | 0.058759 | 0.070645 | 0.104853 | 0.141107* | 0.069196 | 0.056978 |
Lipid Particles | 0.003885 | 0.005766 | 0.001841 | 0.000362 | 0.015565 | 0.002273 | 0.001626 | 0.001133 | 0.009381 | 0.004422 | 0.001817 | 0.000439 |
Mitochondria | 0.484256* | 0.392751* | 0.127247* | 0.086963 | 0.419502* | 0.424178* | 0.014081 | 0.018151 | 0.453788* | 0.404845* | 0.114601* | 0.080122 |
Mitotic Spindle | 0.016496 | 0.018331 | 0.214321* | 0.140142* | 0.026332 | 0.032814 | 0.132466* | 0.166382* | 0.021124 | 0.023905 | 0.205174* | 0.142751* |
None | 0.000809 | 0.001032 | 0.000508 | 0.000280 | 0.003686 | 0.000339 | 0.000138 | 0.000136 | 0.002163 | 0.000765 | 0.000466 | 0.000266 |
Nuclear Periphery | 0.000086 | 0.000051 | 0.000434 | 0.000065 | 0.000273 | 0.000066 | 0.000276 | 0.000118 | 0.000174 | 0.000056 | 0.000417 | 0.000070 |
Nuclear Periphery Foci | 0.000339 | 0.000765 | 0.001292 | 0.000173 | 0.001321 | 0.000678 | 0.001034 | 0.000342 | 0.000801 | 0.000731 | 0.001263 | 0.000190 |
Nucleolus | 0.000415 | 0.001379 | 0.001642 | 0.000091 | 0.006108 | 0.001058 | 0.000621 | 0.000210 | 0.003094 | 0.001255 | 0.001528 | 0.000103 |
Nucleus | 0.000572 | 0.001336 | 0.002308 | 0.000743 | 0.006562 | 0.000636 | 0.004152 | 0.000760 | 0.003390 | 0.001067 | 0.002514 | 0.000745 |
Peroxisomes | 0.073490 | 0.104145* | 0.052429 | 0.064820 | 0.064485 | 0.067993 | 0.152803* | 0.082367 | 0.069253 | 0.090233 | 0.063646 | 0.066564 |
Vacuole | 0.017801 | 0.014122 | 0.070565 | 0.025966 | 0.022321 | 0.014715 | 0.064413 | 0.050722 | 0.019928 | 0.014350 | 0.069877 | 0.028427 |
Vacuole Periphery | 0.012859 | 0.008887 | 0.017546 | 0.007044 | 0.013872 | 0.011522 | 0.009303 | 0.004375 | 0.013336 | 0.009901 | 0.016625 | 0.006778 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.44 | -0.29 | -12.82 | -11.42 | -12.82 | -1.42 | -4.31 | -4.20 | -3.95 | -0.61 | -3.13 | -4.64 | -13.95 | -12.64 | -12.09 |
Bud Neck | -3.83 | -4.57 | 0.83 | 4.68 | 6.51 | 7.34 | 1.25 | 3.95 | -2.23 | 2.72 | 2.09 | 0.08 | 5.83 | 3.11 | 7.10 |
Bud Site | -2.21 | -10.72 | -38.65* | -35.14* | -30.30* | -2.84 | -13.31 | -11.45 | -10.67 | 0.19 | -4.07 | -18.56 | -43.45 | -39.41* | -27.71* |
Cell Periphery | -1.09 | 7.09 | 3.94 | 5.74 | -7.62 | 4.95 | 4.15 | 6.26 | 2.85 | 1.72 | 0.70 | 9.18 | 4.95 | 4.54 | -7.09 |
Cytoplasm | -1.69 | -10.01 | -24.50 | -23.55 | -16.65 | 0.75 | -4.26 | -5.92 | -6.07 | -2.40 | -0.62 | -10.61 | -25.06 | -24.59 | -16.65 |
Cytoplasmic Foci | -5.31 | -11.39 | -0.18 | 4.82 | 10.45 | 1.34 | -2.83 | -4.39 | -4.85 | -2.08 | -2.61 | -9.14 | 1.11 | 3.22 | 8.86 |
Eisosomes | -7.02 | 9.11 | 1.39 | 8.11 | -7.54 | 3.31 | 1.87 | 4.36 | 2.06 | 2.64 | -1.62 | 7.63 | 3.30 | 6.32 | -6.21 |
Endoplasmic Reticulum | -1.88 | -4.32 | -1.88 | 0.08 | 2.11 | 3.96 | -2.96 | -0.22 | -1.47 | 2.00 | 1.06 | -3.92 | -0.59 | -1.30 | 2.80 |
Endosome | 0.78 | -3.73 | 24.61 | 21.34 | 30.12* | 3.24 | 6.63 | 5.93 | 3.63 | 0.26 | 2.68 | -1.98 | 30.75 | 24.00 | 28.37* |
Golgi | -4.65 | 7.20 | 10.79 | 13.79 | 4.59 | -7.80 | 8.09 | 4.91 | 9.42 | -0.80 | -8.26 | 11.51 | 14.56 | 19.54 | 4.00 |
Lipid Particles | -1.94 | 3.28 | 6.90 | 6.27 | 5.48 | 9.13 | 9.53 | 9.82 | 1.03 | 0.90 | 5.58 | 9.87 | 12.48 | 6.88 | 5.61 |
Mitochondria | 7.39 | 38.02* | 43.95* | 29.70* | 6.30 | -0.40 | 46.01* | 41.84* | 31.24* | -0.55 | 5.15 | 46.57* | 53.87* | 38.90* | 5.96 |
Mitotic Spindle | -0.96 | -32.20* | -22.30 | -21.54 | 9.19 | -1.82 | -7.75 | -7.12 | -6.74 | -1.37 | -1.50 | -32.29 | -22.67 | -21.69 | 8.21 |
None | -1.03 | 1.85 | 3.64 | 4.11 | 3.08 | 4.91 | 5.16 | 5.20 | 3.66 | 0.56 | 3.99 | 5.04 | 5.73 | 4.37 | 3.09 |
Nuclear Periphery | 1.53 | -6.54 | 0.39 | -2.57 | 7.17 | 3.75 | -0.54 | 1.11 | -1.75 | 1.14 | 4.22 | -4.93 | 3.18 | -2.09 | 7.20 |
Nuclear Periphery Foci | -2.35 | -4.67 | 2.10 | 3.02 | 5.15 | 0.99 | 0.06 | 1.54 | 0.06 | 2.52 | 0.26 | -2.39 | 2.68 | 2.01 | 5.45 |
Nucleolus | -2.77 | -4.78 | 3.89 | 3.74 | 6.04 | 4.28 | 5.45 | 6.24 | 1.13 | 2.37 | 3.24 | 2.67 | 6.72 | 3.30 | 6.27 |
Nucleus | -2.24 | -6.28 | -3.11 | 0.13 | 3.63 | 6.28 | -0.12 | 4.08 | -1.66 | 2.26 | 4.75 | 0.40 | 4.28 | -0.91 | 4.28 |
Peroxisomes | -5.19 | 5.20 | 2.33 | 7.19 | -2.80 | -0.63 | -6.00 | -1.30 | -0.91 | 4.07 | -5.02 | 1.94 | 1.31 | 5.66 | -0.48 |
Vacuole | 2.71 | -19.31 | -6.55 | -8.31 | 12.26 | 3.81 | -5.75 | -4.97 | -5.69 | 0.41 | 4.84 | -19.55 | -7.39 | -9.92 | 11.19 |
Vacuole Periphery | 4.00 | -3.74 | 5.28 | 1.91 | 8.33 | 1.79 | 2.20 | 6.20 | 4.48 | 1.94 | 4.36 | -2.98 | 7.02 | 3.49 | 8.47 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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