Standard name
Human Ortholog
Description Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p; N-terminally propionylated in vivo
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.99 0.97 0.97 0.92 – 0.88 0.79 0.79 0.68 0.78 1.0 0.99 0.99 – – – 0.87 0.87 0.77 0.87 0.85 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0.13 0 0.2 – 0.21 0.42 0.37 0.51 0.41 0 0 0 – – – 0 0 0.07 0.05 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 – – – 4 0 2 0 2 5
Bud 1 0 0 1 1 6 – 15 8 13 9 11 0 0 1 – – – 5 2 1 0 5 13
Bud Neck 3 1 1 0 3 4 – 22 2 7 3 3 0 0 0 – – – 2 0 0 0 3 4
Bud Site 0 0 0 0 0 0 – 0 0 1 1 7 1 0 0 – – – – – – – – –
Cell Periphery 3 0 0 3 0 0 – 0 2 1 2 3 1 0 1 – – – 2 0 1 2 2 3
Cytoplasm 595 148 178 226 271 365 – 570 308 343 255 422 532 661 744 – – – 508 134 108 167 383 432
Endoplasmic Reticulum 2 1 0 0 1 0 – 0 1 0 0 0 0 1 2 – – – 1 0 0 1 1 4
Endosome 0 0 0 0 0 1 – 1 0 0 0 0 0 0 1 – – – 3 1 1 1 3 7
Golgi 1 1 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 2 2 0 0 3 1
Mitochondria 12 4 3 31 9 79 – 136 164 158 190 225 2 2 0 – – – 22 6 10 8 20 21
Nucleus 1 0 0 0 0 0 – 2 1 0 0 3 0 0 1 – – – 0 0 0 0 0 1
Nuclear Periphery 1 0 0 0 0 0 – 1 0 0 0 1 0 1 1 – – – 0 0 0 0 0 1
Nucleolus 0 0 0 2 0 0 – 0 1 1 0 1 0 0 0 – – – 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 – – – 0 0 0 0 0 1
SpindlePole 1 0 0 1 5 3 – 24 4 6 5 14 1 2 0 – – – 6 0 1 1 4 13
Vac/Vac Membrane 0 0 0 0 1 4 – 1 1 0 1 2 0 0 0 – – – 7 3 2 2 10 16
Unique Cell Count 610 152 179 232 279 398 649 392 432 373 543 534 665 748 582 154 140 193 449 545
Labelled Cell Count 620 155 182 264 291 462 773 492 530 467 692 537 667 751 582 154 140 193 449 545


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.0 2.8 2.9 3.5 2.8 – 2.9 2.2 2.6 2.5 2.4 4.0 4.0 4.0 4.6 4.4 4.6 3.6 4.0 4.2
Std Deviation (1e-4) 0.6 1.0 0.7 1.7 1.8 1.3 – 1.3 0.8 1.0 0.9 0.9 1.4 1.1 1.3 1.3 1.1 1.7 0.6 1.2 1.8
Intensity Change (Log2) – – – 0.05 0.32 -0.0 – 0.06 -0.31 -0.11 -0.16 -0.2 0.53 0.55 0.53 0.72 0.66 0.74 0.38 0.51 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5464 -0.2754 -0.6241 -0.3826 -0.3272 -0.5999 -1.3787 -1.1759 -1.348 -1.6426 -1.495 -1.2122 -0.0987 0.1159 0.2791 0.4529 0.6906 0.177
Actin 0.0348 0.0151 0.026 0.0195 0.0324 0.01 0.0259 0.0003 0.0164 0.0003 0.0007 0.0021 0.0138 0.0031 0.0091 0.034 0.011 0.0324
Bud 0.0006 0.004 0.0004 0.0011 0.0003 0.0004 0.0004 0.0041 0.0008 0.0001 0.0002 0.0001 0.0004 0.0044 0.0009 0.0015 0.0133 0.0017
Bud Neck 0.0019 0.0004 0.0006 0.0005 0.003 0.0007 0.0027 0.0001 0.0018 0 0.0004 0.0002 0.0133 0.0002 0.0008 0.0045 0.0024 0.0013
Bud Periphery 0.0008 0.0035 0.0007 0.0056 0.0004 0.0008 0.0008 0.0007 0.0012 0.0005 0.0004 0.0003 0.0008 0.006 0.0019 0.0063 0.0148 0.0078
Bud Site 0.0039 0.0064 0.0077 0.0028 0.0014 0.0001 0.0043 0.0088 0.0127 0 0.0053 0.0001 0.0096 0.0111 0.0138 0.0017 0.0164 0.0006
Cell Periphery 0.0003 0.0002 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0003 0 0.0001 0.0001 0.0004 0.0002 0.0002 0.0007 0.0007 0.0004
Cytoplasm 0.0308 0.0178 0.0212 0.0095 0.0365 0.057 0.0297 0.0363 0.0286 0.0098 0.0151 0.0483 0.0141 0.0295 0.03 0.0393 0.0107 0.043
Cytoplasmic Foci 0.031 0.0092 0.0174 0.0069 0.0232 0.0089 0.0284 0.006 0.0159 0.0063 0.0074 0.0073 0.0076 0.0011 0.0121 0.0187 0.033 0.005
Eisosomes 0.0006 0.0002 0.0004 0.0008 0.0003 0.0002 0.0006 0.0003 0.0004 0.0001 0.0001 0.0001 0.0003 0.0002 0.0002 0.0002 0.0002 0.0004
Endoplasmic Reticulum 0.0032 0.0005 0.0016 0.0015 0.006 0.0007 0.0024 0.0004 0.0011 0.0001 0.0006 0.0004 0.0006 0.0004 0.001 0.0014 0.0104 0.0009
Endosome 0.0086 0.0003 0.003 0.0167 0.0487 0.0019 0.0176 0.0003 0.0051 0.0002 0.0048 0.0043 0.0104 0.0005 0.0117 0.0153 0.0231 0.0044
Golgi 0.0037 0.0006 0.0015 0.0053 0.0171 0.0018 0.0062 0.0001 0.0038 0.0001 0.0016 0.0037 0.0056 0.0003 0.0025 0.0073 0.0114 0.0022
Lipid Particles 0.0092 0.0008 0.0021 0.0089 0.0351 0.0049 0.0151 0.0002 0.0032 0.0006 0.0072 0.0022 0.005 0.0001 0.0043 0.0106 0.0464 0.0018
Mitochondria 0.0112 0.0009 0.0011 0.0225 0.0106 0.0013 0.0037 0.0007 0.0121 0.0018 0.002 0.0059 0.0027 0.0014 0.0016 0.0027 0.003 0.0022
None 0.8187 0.9308 0.8979 0.8692 0.7086 0.89 0.8255 0.936 0.8825 0.9736 0.9344 0.9129 0.8961 0.9405 0.899 0.8251 0.7179 0.8867
Nuclear Periphery 0.009 0.0006 0.0015 0.0063 0.0256 0.0023 0.0052 0.0002 0.0005 0.0002 0.0024 0.0037 0.0008 0.0002 0.001 0.0009 0.007 0.0026
Nucleolus 0.001 0.001 0.0003 0.0015 0.0008 0.0004 0.0007 0.001 0.0004 0.0003 0.0009 0.0003 0.0014 0.0002 0.0004 0.0006 0.0014 0.0004
Nucleus 0.0057 0.0023 0.0008 0.0051 0.0047 0.0063 0.0015 0.0014 0.0004 0.0003 0.0012 0.0027 0.0043 0.0003 0.0008 0.001 0.0073 0.0013
Peroxisomes 0.0115 0.0008 0.0096 0.0045 0.0313 0.0096 0.0207 0.0003 0.0083 0.0036 0.0122 0.0023 0.0071 0.0001 0.0022 0.0232 0.0549 0.0018
Punctate Nuclear 0.012 0.0039 0.0054 0.009 0.0068 0.002 0.0041 0.0023 0.0033 0.0018 0.002 0.0023 0.002 0.0002 0.004 0.001 0.0055 0.0022
Vacuole 0.0011 0.0006 0.0003 0.0018 0.0028 0.0003 0.0033 0.0003 0.0008 0.0001 0.0007 0.0006 0.0031 0.0002 0.0019 0.0033 0.0073 0.0006
Vacuole Periphery 0.0006 0.0001 0.0001 0.0009 0.0043 0.0003 0.0009 0.0001 0.0004 0 0.0003 0.0003 0.0007 0.0001 0.0007 0.0006 0.0019 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1433.8727 1048.1792 139.4159 99.628 1077.4409 762.8347 558.0265 304.5096 250.5358 881.7015
Translational Efficiency 2.2165 2.0079 1.6007 2.6634 2.3982 3.2635 2.2362 2.2948 1.7935 2.3991

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1754 1233 304 1293 1286 1652 2432 1400 3040 2885 2736 2693

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 639.55 703.43 918.74 778.14 617.13 754.28 762.93 804.89 630.07 732.55 780.24 792.05
Standard Deviation 80.14 93.17 103.40 112.79 67.67 94.39 100.75 99.68 75.93 97.18 112.29 107.02
Intensity Change Log 2 — 0.137350 0.522599 0.282973 — 0.289526 0.305976 0.383217 — 0.214086 0.420277 0.333071

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000056 0.000272 0.000867 0.000783 0.000056 0.000794 0.000168 0.000818 0.000056 0.000571 0.000246 0.000801
Bud Neck 0.012663 0.021641 0.003826 0.010205 0.010404 0.028064 0.009934 0.007009 0.011707 0.025319 0.009255 0.008544
Bud Site 0.001653 0.006480 0.003612 0.036626 0.002858 0.008938 0.016013 0.036396 0.002163 0.007887 0.014635 0.036506
Cell Periphery 0.000106 0.000146 0.000202 0.000288 0.000278 0.000222 0.000117 0.000196 0.000179 0.000189 0.000126 0.000240
Cytoplasm 0.485055* 0.437501* 0.114221* 0.399950* 0.450126* 0.365514* 0.500020* 0.438111* 0.470279* 0.396280* 0.457154* 0.419788*
Cytoplasmic Foci 0.205846* 0.230723* 0.005709 0.033577 0.213834* 0.222229* 0.009718 0.034811 0.209225* 0.225859* 0.009273 0.034219
Eisosomes 0.000160 0.000131 0.000085 0.000082 0.000313 0.000189 0.000024 0.000065 0.000225 0.000164 0.000031 0.000073
Endoplasmic Reticulum 0.000474 0.000766 0.011244 0.002008 0.000407 0.000890 0.000873 0.001599 0.000445 0.000837 0.002025 0.001795
Endosome 0.010965 0.016683 0.003092 0.011788 0.005719 0.017666 0.001858 0.005608 0.008746 0.017246 0.001995 0.008575
Golgi 0.001188 0.002708 0.000112 0.006349 0.001154 0.003804 0.000210 0.001790 0.001174 0.003336 0.000199 0.003979
Lipid Particles 0.011396 0.007707 0.002800 0.002325 0.015336 0.008839 0.000625 0.001945 0.013063 0.008355 0.000866 0.002127
Mitochondria 0.003568 0.006335 0.000762 0.009190 0.005798 0.014168 0.001646 0.009547 0.004511 0.010820 0.001548 0.009376
Mitotic Spindle 0.000450 0.003752 0.004137 0.033805 0.000698 0.004563 0.026479 0.019417 0.000555 0.004216 0.023996 0.026325
None 0.005390 0.003027 0.004149 0.009777 0.004131 0.002667 0.005673 0.002923 0.004857 0.002821 0.005503 0.006214
Nuclear Periphery 0.000556 0.000610 0.002588 0.001987 0.000575 0.000663 0.001386 0.001727 0.000564 0.000640 0.001520 0.001852
Nuclear Periphery Foci 0.000281 0.000886 0.001477 0.001260 0.000179 0.000285 0.000230 0.000705 0.000238 0.000542 0.000369 0.000971
Nucleolus 0.002697 0.001733 0.001294 0.001290 0.001432 0.001784 0.000439 0.000448 0.002162 0.001762 0.000534 0.000852
Nucleus 0.206000* 0.181637* 0.791819* 0.309178* 0.255964* 0.238438* 0.388923* 0.304482* 0.227136* 0.214162* 0.433689* 0.306736*
Peroxisomes 0.005049 0.015003 0.000395 0.002879 0.005021 0.016624 0.001483 0.000671 0.005037 0.015931 0.001362 0.001731
Vacuole 0.045910 0.060557 0.047384 0.122225 0.025100 0.061819 0.033888 0.129695 0.037107 0.061280 0.035387 0.126108
Vacuole Periphery 0.000539 0.001702 0.000226 0.004428 0.000618 0.001840 0.000294 0.002040 0.000572 0.001781 0.000286 0.003186

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.69 -11.04 -4.40 -2.00 3.96 -1.95 -4.24 -2.87 0.60 -1.97 -2.43 -7.67 -4.75 0.14 -2.21
Bud Neck -4.89 6.17 3.67 7.38 -1.93 -8.70 0.25 3.78 11.50 3.68 -9.75 2.08 5.57 13.52 2.67
Bud Site -6.61 -2.90 -8.55 -6.56 -6.76 -4.37 -5.68 -6.74 -4.21 -3.03 -7.17 -7.02 -10.94 -7.47 -5.27
Cell Periphery -3.75 -6.64 -5.67 -3.04 0.35 1.52 5.95 4.37 2.79 -3.05 -0.53 4.53 0.35 0.89 -5.02
Cytoplasm 4.97 38.25 13.21 7.45 -23.50 8.69 1.57 8.63 0.37 7.92 10.76 9.71 15.78 5.11 5.19
Cytoplasmic Foci -3.17 42.17 36.39 32.28 -13.92 -1.01 37.73 33.57 34.52 -11.47 -2.96 55.95 49.51 47.20 -16.17
Eisosomes 1.86 3.78 5.63 8.09 2.65 2.81 6.54 5.49 11.87 -16.28 2.89 9.34 7.31 14.08 -15.56
Endoplasmic Reticulum -2.91 -13.58 -12.44 -9.61 11.49 -2.68 -12.14 -14.87 -7.48 -6.73 -3.56 -16.00 -19.18 -11.46 2.61
Endosome -3.65 8.51 3.25 6.02 -3.94 -9.24 8.78 3.67 10.95 -4.24 -8.26 13.35 5.13 11.83 -7.16
Golgi -3.04 7.06 -2.26 -0.61 -3.44 -4.65 6.77 1.23 4.94 -2.38 -5.54 9.34 -1.71 2.21 -3.97
Lipid Particles 3.60 10.29 11.58 8.38 2.40 5.04 12.22 11.13 13.84 -7.15 5.99 17.75 16.03 15.74 -9.38
Mitochondria -2.27 4.20 -1.07 0.65 -3.21 -3.88 3.17 0.77 4.54 -2.04 -4.99 4.42 -0.17 4.26 -3.39
Mitotic Spindle -2.95 -2.28 -7.58 -6.20 -3.95 -3.19 -10.81 -5.78 -3.64 4.48 -4.51 -11.33 -9.56 -7.11 2.16
None 2.50 2.43 -0.98 -3.03 -2.98 2.12 -2.36 2.83 0.02 8.17 3.34 -0.59 0.40 -2.73 1.05
Nuclear Periphery -0.58 -14.86 -12.15 -11.04 2.42 -2.00 -20.75 -11.86 -11.35 -2.34 -1.70 -25.60 -16.95 -15.89 -3.03
Nuclear Periphery Foci -1.60 -7.62 -5.12 -0.74 2.69 -1.53 -3.18 -6.82 -4.98 -5.73 -1.82 -6.18 -7.83 -2.51 -5.26
Nucleolus 1.73 3.09 3.51 2.43 1.21 -0.85 4.48 4.95 4.29 1.76 1.19 5.59 5.08 4.70 -1.29
Nucleus 3.19 -42.42 -14.21 -15.73 29.69 1.87 -20.32 -6.91 -8.90 12.25 2.09 -35.21 -15.51 -16.64 18.57
Peroxisomes -6.51 9.81 5.21 8.79 -2.72 -8.73 6.06 8.12 13.53 1.47 -10.92 8.57 9.50 15.69 -0.28
Vacuole -5.03 -4.23 -21.84 -18.21 -12.79 -14.01 -12.02 -29.78 -23.06 -24.67 -11.76 -8.23 -36.03 -29.28 -30.27
Vacuole Periphery -2.47 7.11 -3.37 -1.04 -3.89 -4.50 3.93 -1.09 2.40 -2.56 -4.81 6.65 -3.51 0.30 -4.66
Standard name
Human Ortholog
Description Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p; N-terminally propionylated in vivo
Localization
Cell Percentages cytoplasm (22%), nucleus (8%), mixed (31%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Hta1

Hta1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hta1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available