Standard name
Human Ortholog
Description Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress; mutations affecting Adk1p catalytic activity deregulate expression of phosphate utilization genes PHO5 and PHO84; human homolog AK1 can complement yeast adk1 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.99 0.97 0.91 0.9 0.88 0.92 0.88 0.92 0.88 0.99 0.99 0.99 0.99 0.99 0.99 0.96 0.96 0.96 0.95 0.95 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0.07 0.09 0.05 0.09 0 0.1 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 1 0 0 1 1 0 0 4 1 0 0 1 1 4 0 4 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 4 1 5 8 8 16 13 24 9 10 2 2 3 5 8 2 0 0 0 0 0 0
Cytoplasm 360 304 526 380 393 285 371 542 354 502 225 212 356 539 571 546 447 508 333 306 514 291 468 515
Endoplasmic Reticulum 1 3 1 5 11 14 28 57 19 50 9 23 4 3 3 17 17 25 1 0 2 0 5 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Golgi 0 0 2 0 0 0 2 2 1 1 3 3 0 0 0 0 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleus 0 0 0 0 0 2 0 1 1 0 0 1 0 0 0 0 0 0 2 2 1 2 1 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 0 0 5 3 2 3 2 1 2 1 1 1 5 4 3 1 2 1 3 5 6
Unique Cell Count 362 307 530 385 406 312 411 616 386 569 244 242 360 543 578 554 453 512 346 318 533 306 492 539
Labelled Cell Count 364 310 533 386 409 316 413 621 395 581 250 252 363 545 583 574 478 538 346 318 533 306 492 539


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 121.1 118.8 124.7 113.0 101.9 65.2 75.9 73.1 68.6 67.2 62.3 63.2 133.9 130.4 133.0 152.4 163.4 144.9 106.6 115.3 122.8
Std Deviation (1e-4) 30.0 29.4 25.5 24.2 22.5 23.4 16.2 17.4 16.9 16.4 16.7 16.0 42.4 35.6 30.7 48.5 52.8 45.9 29.9 29.8 30.4
Intensity Change (Log2) – – – -0.14 -0.29 -0.94 -0.72 -0.77 -0.86 -0.89 -1.0 -0.98 0.1 0.06 0.09 0.29 0.39 0.22 -0.23 -0.11 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1.6 2.4 2.9 3.6 0 0 0 0 0 0 1.4 0
Cytoplasm -0.8 -2.8 -5.9 -6.5 -7.5 -5.8 -7.4 -5.4 -7.3 -0.6 0 -0.8 -1.1 -0.9 0
Endoplasmic Reticulum 0 3.4 4.6 5.8 7.0 4.8 6.8 0 6.9 0 0 0 3.7 4.2 4.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 71.0849 103.7059 90.827 98.5705 89.995 98.5482 106.7867 119.3117 115.4978 107.6903 107.298 114.8243 122.2732 135.0805 137.6322 122.8823 125.5775 123.2696
Actin 0.0199 0.0006 0.0129 0 0.0001 0.0002 0.0007 0.0003 0.0001 0.0001 0 0 0.0001 0.0003 0.0001 0 0.0001 0.0002
Bud 0.0027 0 0.0005 0 0.0001 0.0001 0.0007 0.0001 0 0 0 0 0.0001 0.0002 0 0 0 0.0001
Bud Neck 0.0041 0.0002 0.0004 0.0001 0.0006 0.0006 0.0002 0.0002 0.0001 0.0002 0.0002 0.0004 0.0001 0.0001 0.0001 0.0001 0.0002 0.0004
Bud Periphery 0.0019 0 0.0003 0 0 0 0.0003 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0.0085 0.0047 0.0011 0 0.0004 0 0.0007 0 0 0 0 0 0.0001 0.0001 0 0 0 0
Cell Periphery 0.0011 0.0001 0.0006 0 0 0 0.0015 0.0022 0 0 0 0 0.0001 0.0004 0 0 0 0
Cytoplasm 0.8125 0.9843 0.8842 0.99 0.9222 0.9846 0.9332 0.9867 0.991 0.9792 0.9885 0.9583 0.9545 0.9944 0.9942 0.9919 0.9901 0.9879
Cytoplasmic Foci 0.0169 0.0001 0.0266 0.0001 0.0058 0.0001 0.0021 0.0005 0.0001 0.0001 0.0001 0.0001 0.0023 0.0001 0.0001 0.0001 0.0001 0.0003
Eisosomes 0.0004 0 0.0003 0 0 0 0.0002 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0024 0.0004 0.0014 0.0002 0.0002 0.0002 0.0017 0.0004 0.0001 0.0004 0.0002 0.0003 0.0002 0.0001 0 0 0.0001 0.0001
Endosome 0.0101 0.0002 0.0039 0 0.0494 0 0.0007 0 0 0 0 0 0.0001 0 0 0 0 0.0001
Golgi 0.0025 0 0.003 0 0.0061 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Lipid Particles 0.0066 0.0001 0.0036 0 0.0039 0 0.0016 0.0005 0 0 0 0 0.0004 0 0 0 0 0
Mitochondria 0.0107 0.0002 0.0056 0 0.0012 0.0001 0.0012 0.0001 0 0 0 0 0.002 0.0002 0 0 0.0001 0.0001
None 0.0168 0.0002 0.005 0.0001 0.0001 0.0003 0.0051 0.0017 0.0001 0.0005 0.0001 0.0001 0.0012 0.0002 0.0001 0.0001 0.0001 0.0002
Nuclear Periphery 0.0095 0.0031 0.0033 0.0013 0.0008 0.0015 0.0078 0.0015 0.0011 0.0027 0.0015 0.0035 0.0028 0.0006 0.0007 0.0006 0.0011 0.0011
Nucleolus 0.0199 0.0001 0.0165 0 0.0001 0 0.0074 0.0001 0 0 0 0 0.0076 0.0001 0 0 0 0
Nucleus 0.0295 0.0052 0.0147 0.0079 0.005 0.0118 0.0269 0.0046 0.007 0.0161 0.0083 0.0369 0.0242 0.0028 0.0045 0.007 0.0079 0.0091
Peroxisomes 0.0045 0 0.0012 0 0.0006 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0
Punctate Nuclear 0.0109 0.0003 0.0058 0 0.0001 0.0001 0.0012 0.0001 0 0.0001 0.0001 0.0001 0.0027 0.0001 0 0 0 0.0001
Vacuole 0.0067 0.0002 0.0055 0.0002 0.0026 0.0003 0.0052 0.001 0.0002 0.0004 0.0007 0.0003 0.0008 0.0002 0 0.0001 0.0002 0.0003
Vacuole Periphery 0.0017 0.0001 0.0037 0.0001 0.0006 0.0001 0.0012 0.0001 0 0 0.0001 0 0.0005 0 0 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 624.2729 628.096 595.1748 571.4488 553.8543 728.602 680.5357 682.685 704.8339 620.2966
Translational Efficiency 1.948 1.9738 1.9665 2.0143 2.2741 2.1616 2.004 2.103 2.1785 2.182

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
99 860 1284 1828 2126 2711 2069 2048 2225 3571 3353 3876

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 7688.62 7842.43 8008.38 9262.43 6377.31 7341.77 8550.91 9683.67 6435.66 7462.34 8343.15 9485.00
Standard Deviation 2666.54 1784.48 1633.94 2135.18 1355.45 1665.80 1863.41 2186.42 1464.57 1708.60 1798.47 2172.61
Intensity Change Log 2 — 0.028576 0.058786 0.268666 — 0.203180 0.423130 0.602606 — 0.110366 0.235436 0.429696

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000553 0.000065 0.000064 0.000263 0.000043 0.000039 0.000037 0.000153 0.000066 0.000045 0.000047 0.000205
Bud Neck 0.007565 0.000523 0.000812 0.001393 0.000304 0.000298 0.000312 0.000827 0.000627 0.000352 0.000503 0.001094
Bud Site 0.008504 0.000714 0.001164 0.003618 0.000867 0.001552 0.001101 0.003584 0.001207 0.001350 0.001125 0.003600
Cell Periphery 0.033464 0.001394 0.000939 0.000686 0.000400 0.000180 0.000543 0.000430 0.001871 0.000472 0.000695 0.000551
Cytoplasm 0.521893* 0.731003* 0.750641* 0.828025* 0.786138* 0.875815* 0.856792* 0.860033* 0.774381* 0.840940* 0.816142* 0.844937*
Cytoplasmic Foci 0.048064 0.007531 0.006668 0.005010 0.003646 0.003170 0.001891 0.003470 0.005623 0.004220 0.003721 0.004196
Eisosomes 0.000021 0.000001 0.000001 0.000003 0.000000 0.000001 0.000001 0.000001 0.000001 0.000001 0.000001 0.000002
Endoplasmic Reticulum 0.000870 0.000022 0.000016 0.000041 0.000023 0.000015 0.000006 0.000015 0.000061 0.000017 0.000010 0.000027
Endosome 0.001161 0.000033 0.000048 0.000271 0.000035 0.000032 0.000013 0.000128 0.000085 0.000033 0.000026 0.000196
Golgi 0.008271 0.000685 0.000375 0.000515 0.000478 0.000274 0.000101 0.000169 0.000824 0.000373 0.000206 0.000332
Lipid Particles 0.022071 0.000644 0.000526 0.000512 0.000259 0.000168 0.000290 0.000745 0.001229 0.000282 0.000380 0.000635
Mitochondria 0.001449 0.000160 0.000137 0.000171 0.000144 0.000124 0.000071 0.000138 0.000202 0.000133 0.000096 0.000154
Mitotic Spindle 0.002492 0.000490 0.000317 0.000371 0.000634 0.000525 0.000060 0.001074 0.000716 0.000517 0.000159 0.000742
None 0.019053 0.017763 0.007898 0.008048 0.014116 0.013997 0.010165 0.010079 0.014336 0.014904 0.009297 0.009121
Nuclear Periphery 0.001242 0.000137 0.000171 0.000101 0.000073 0.000030 0.000051 0.000067 0.000125 0.000056 0.000097 0.000083
Nuclear Periphery Foci 0.008981 0.000622 0.000560 0.000500 0.000456 0.000241 0.000320 0.000437 0.000836 0.000333 0.000412 0.000467
Nucleolus 0.002444 0.000100 0.000066 0.000174 0.000057 0.000067 0.000066 0.000100 0.000163 0.000075 0.000066 0.000134
Nucleus 0.255552* 0.233805* 0.221564* 0.134367* 0.189602* 0.099949 0.117067 0.104120 0.192536* 0.132185* 0.157083* 0.118385
Peroxisomes 0.000452 0.000006 0.000006 0.000122 0.000012 0.000028 0.000013 0.000027 0.000031 0.000023 0.000010 0.000072
Vacuole 0.046602 0.004075 0.007848 0.015425 0.002457 0.003284 0.011018 0.014323 0.004421 0.003474 0.009804 0.014843
Vacuole Periphery 0.009296 0.000227 0.000181 0.000383 0.000256 0.000212 0.000081 0.000081 0.000659 0.000216 0.000119 0.000224

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 5.12 4.95 3.25 -1.59 -1.34 0.85 -1.26 -2.30 -2.51 -2.09 2.18 -0.27 -2.33 -2.89 -2.31
Bud Neck 2.81 2.58 2.55 -3.67 -0.36 1.60 -3.72 -7.52 -8.64 -5.80 2.39 -0.90 -3.17 -8.97 -3.71
Bud Site 3.36 2.96 2.28 -5.59 -3.31 -2.01 -3.47 -5.54 -3.72 -4.48 -0.31 -1.66 -6.10 -5.51 -5.68
Cell Periphery 4.70 4.77 4.86 3.87 4.35 4.40 -1.96 -2.23 -7.83 0.82 3.94 2.89 3.78 -0.81 3.01
Cytoplasm -8.29 -7.75 -10.78 -7.79 -11.07 -19.48 -10.13 -6.80 12.83 3.17 -13.62 -2.78 -6.38 9.32 -4.22
Cytoplasmic Foci 6.43 6.05 6.85 1.98 5.04 1.76 0.77 -5.45 -7.93 -6.39 2.38 -1.18 -1.53 -4.89 -0.41
Eisosomes 4.23 4.11 3.96 -2.60 -1.48 -1.32 -5.50 -8.38 -7.70 -5.60 2.67 0.43 -2.58 -6.49 -4.07
Endoplasmic Reticulum 1.63 1.64 1.62 -0.40 -1.17 4.64 11.82 4.77 1.73 -2.24 1.97 2.35 1.87 -0.32 -2.10
Endosome 1.73 1.71 1.49 -2.00 -1.74 1.32 4.08 -0.97 -1.26 -1.77 1.96 2.12 -0.87 -2.44 -2.56
Golgi 5.01 5.31 5.33 2.25 0.41 3.19 5.95 5.19 3.01 -1.06 4.27 6.40 5.76 2.37 -0.45
Lipid Particles 2.67 2.64 2.65 -0.70 0.28 2.08 -1.88 -3.56 -4.50 -2.45 2.55 1.21 0.20 -5.53 -2.32
Mitochondria 2.20 2.25 2.28 1.61 0.62 2.64 11.03 2.29 0.97 -1.51 2.66 4.24 3.07 1.15 -1.25
Mitotic Spindle 1.19 1.65 1.50 0.39 -0.78 1.14 3.51 0.54 -0.09 -1.21 1.13 3.34 1.03 0.07 -1.54
None -0.14 0.85 0.96 7.52 1.83 0.18 4.98 6.70 6.00 1.19 -0.41 6.09 7.61 9.16 1.99
Nuclear Periphery 5.97 5.26 5.94 -0.48 5.53 8.92 -0.31 -5.48 -16.17 -5.67 5.90 -2.40 -1.29 -11.10 1.70
Nuclear Periphery Foci 2.85 2.80 2.78 -1.23 -0.34 3.90 0.13 -4.08 -10.50 -6.03 3.51 1.40 -0.26 -9.19 -4.18
Nucleolus 1.31 1.33 1.28 -1.19 -3.60 -1.04 -1.91 -2.88 -1.94 -1.96 1.06 1.10 0.41 -3.01 -4.21
Nucleus 1.71 1.45 5.73 11.53 14.45 20.46 12.87 14.72 -5.92 2.29 13.67 4.53 13.11 -1.33 10.28
Peroxisomes 1.38 1.37 1.12 -1.67 -1.63 -3.41 0.06 -1.95 1.87 -3.26 0.34 1.40 -0.78 -1.17 -1.89
Vacuole 6.13 4.00 1.95 -15.68 -6.72 -2.21 -15.61 -19.94 -19.53 -9.14 1.19 -15.47 -24.16 -26.06 -11.52
Vacuole Periphery 2.93 2.96 2.93 0.05 -0.89 1.08 4.92 5.04 4.10 0.88 2.99 3.89 3.48 1.49 -1.04
Standard name
Human Ortholog
Description Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress; mutations affecting Adk1p catalytic activity deregulate expression of phosphate utilization genes PHO5 and PHO84; human homolog AK1 can complement yeast adk1 mutant
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Adk1

Adk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Adk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available