Standard name
Human Ortholog
Description mRNA 3' end processing factor; essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0.14 0.2 0.08 0 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0.05 0 0 0.06 0.06 – 0.2 0.11 0.3 0.19 0 0 0 0.06 0.06 0.08 0 0 0 0 0 0
Nucleus 0.93 0.92 1.0 0.95 0.96 0.92 0.91 – 0.84 0.87 0.83 0.88 0.94 0.85 0.76 0.8 0.84 0.83 0.88 0.88 0.87 0.88 0.87 0.85
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.17 0 0 0 0 0.05 0 – 0.14 0.08 0.17 0.15 0.25 0 0.05 0 0.08 0 0.07 0 0.05 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 2 – 0 0 0 1 0 0 0 0 0 0 3 2 0 0 0 2
Bud Neck 0 0 0 0 0 0 1 – 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 2 5 – 4 6 4 5 0 0 0 0 2 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 4 11 0 3 5 3 12 – 2 8 3 5 1 19 31 11 3 10 0 2 0 0 0 3
Endoplasmic Reticulum 0 1 0 0 1 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Endosome 0 1 0 1 1 1 0 – 2 0 0 0 0 0 0 0 0 0 1 2 0 0 1 2
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 3 2 0 2 1 0 0 1 1
Mitochondria 28 0 1 1 1 12 19 – 37 31 62 39 0 0 0 8 7 8 0 2 1 0 1 2
Nucleus 253 215 20 139 196 182 268 – 156 248 172 181 30 112 116 116 106 85 251 216 39 36 134 192
Nuclear Periphery 5 1 0 0 1 0 1 – 1 1 0 0 0 0 1 0 1 1 0 0 0 0 0 0
Nucleolus 47 7 0 1 2 10 11 – 25 22 35 31 8 3 7 4 10 3 21 7 2 1 5 8
Peroxisomes 0 1 0 0 0 0 0 – 0 0 0 1 0 0 1 1 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 3 0 1 4 – 0 4 0 3 0 0 2 1 0 1 0 2 0 0 0 1
Vac/Vac Membrane 1 5 0 1 1 2 4 – 1 4 1 0 0 0 2 9 3 2 3 4 0 0 6 8
Unique Cell Count 272 233 20 146 205 198 296 185 284 208 206 32 132 152 145 126 103 286 245 45 42 154 227
Labelled Cell Count 338 243 21 149 209 213 327 228 325 278 266 39 134 160 154 135 111 286 245 45 42 154 227


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 7.0 6.0 6.9 7.4 5.9 6.1 – 5.2 5.6 4.8 5.0 5.9 7.4 6.8 11.9 10.2 9.6 5.7 6.8 7.0
Std Deviation (1e-4) 1.1 1.3 0.5 1.7 1.7 1.3 1.8 – 1.1 1.3 1.0 1.2 0.7 1.4 1.6 4.2 3.1 2.7 0.6 1.4 1.1
Intensity Change (Log2) – – – 0.19 0.3 -0.03 0.03 – -0.21 -0.11 -0.32 -0.26 -0.03 0.3 0.17 0.98 0.75 0.68 -0.09 0.17 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus -1.0 -1.0 -1.3 -1.4 – -1.9 -1.7 -2.0 -1.7 -1.1 -1.9 -2.4 -2.2 -1.9 -2.0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5366 1.6711 1.5236 1.0316 1.6842 1.6295 2.63 3.1311 2.8405 2.5204 1.8524 2.74 1.7408 2.078 1.2604 0.9695 1.5117 1.5215
Actin 0.0035 0.0003 0.0246 0.0004 0.0128 0.0021 0.0233 0 0.0102 0.0246 0.0003 0.0111 0.0487 0 0.0008 0.0003 0 0.0074
Bud 0.0003 0.0005 0.0002 0.0004 0.0018 0.0003 0.0008 0 0.0003 0.0001 0 0 0.001 0 0.0002 0.0001 0 0.0002
Bud Neck 0.0013 0.0005 0.0007 0.0004 0.0011 0.006 0.0012 0 0.002 0.0002 0.0002 0.0006 0.0026 0 0.0004 0.0001 0.0002 0.0006
Bud Periphery 0.0003 0.0004 0.0003 0.0014 0.0025 0.0006 0.0012 0 0.0007 0.0001 0.0001 0 0.0016 0 0.0002 0.0002 0 0.0003
Bud Site 0.0036 0.0071 0.0018 0.0011 0.0092 0.0011 0.0052 0 0.0019 0.0015 0.0001 0.0001 0.0159 0 0.0007 0.0002 0 0.0004
Cell Periphery 0.0002 0.0001 0.0001 0.0006 0.0003 0.0002 0.0002 0 0.0002 0.0001 0 0 0.0007 0 0.0001 0.0001 0 0
Cytoplasm 0.0015 0.0001 0.0002 0.0005 0.0009 0.0006 0.0074 0 0.0038 0.0018 0.0002 0.0001 0.0065 0 0.0005 0.0005 0.0002 0.0028
Cytoplasmic Foci 0.0285 0.0043 0.0054 0.0203 0.0047 0.0022 0.014 0 0.0185 0.0304 0.0129 0.0012 0.0212 0 0.0011 0.0073 0 0.0125
Eisosomes 0.0001 0 0.0002 0.0001 0.0002 0 0.0005 0 0.0001 0.0003 0 0 0.0005 0 0 0 0 0
Endoplasmic Reticulum 0.0002 0 0.0002 0.0007 0.0004 0.0003 0.0036 0 0.0022 0.001 0.0001 0 0.0019 0 0.0001 0.0007 0 0.0012
Endosome 0.0079 0.0001 0.005 0.0068 0.012 0.0068 0.0241 0 0.0191 0.051 0.0316 0.0008 0.0223 0 0.0029 0.1444 0 0.0131
Golgi 0.0039 0.0002 0.005 0.0016 0.0092 0.0037 0.0057 0 0.004 0.0112 0.0126 0.0026 0.0091 0 0.0068 0.0239 0 0.0028
Lipid Particles 0.0114 0.0005 0.0073 0.0498 0.002 0.0008 0.022 0 0.0156 0.0315 0.0082 0.0008 0.0294 0 0.0005 0.0191 0 0.0185
Mitochondria 0.0048 0.0005 0.0104 0.0585 0.0615 0.0097 0.0038 0.0001 0.0086 0.0048 0.0012 0.0002 0.0084 0.0001 0.0015 0.0225 0.0001 0.002
None 0.0046 0 0.0002 0.0006 0.0004 0.0001 0.0141 0 0.0016 0.0009 0.0001 0 0.0071 0 0.0001 0.0005 0 0.0032
Nuclear Periphery 0.0011 0.0003 0.0004 0.0032 0.0007 0.002 0.0099 0.0006 0.015 0.0027 0.0007 0.0001 0.0061 0.0002 0.0005 0.0014 0.0025 0.0117
Nucleolus 0.0291 0.0151 0.0083 0.0267 0.081 0.0784 0.0171 0.0158 0.021 0.0045 0.0749 0.0704 0.0205 0.0104 0.0111 0.0049 0.0261 0.0516
Nucleus 0.869 0.9674 0.9209 0.8075 0.7923 0.8804 0.824 0.9829 0.8513 0.8186 0.8522 0.9106 0.7634 0.9889 0.9717 0.7453 0.9703 0.8558
Peroxisomes 0.0194 0.0022 0.0079 0.0033 0.0035 0.0006 0.0062 0 0.0101 0.0078 0.0031 0.001 0.0133 0 0.0003 0.007 0 0.0072
Punctate Nuclear 0.0081 0.0002 0.0005 0.0029 0.0009 0.0009 0.0105 0.0003 0.0084 0.0039 0.0009 0.0003 0.0151 0.0001 0.0004 0.0003 0.0003 0.0068
Vacuole 0.0009 0.0002 0.0002 0.0084 0.0016 0.0024 0.0038 0 0.0034 0.0021 0.0003 0.0001 0.0036 0.0001 0.0002 0.0092 0.0001 0.0008
Vacuole Periphery 0.0004 0 0.0001 0.0047 0.001 0.0009 0.0013 0 0.0018 0.0008 0.0003 0 0.0012 0 0.0001 0.012 0.0001 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.3187 15.2073 8.1715 16.5482 16.352 11.1393 17.0993 16.1035 14.4768 18.0572
Translational Efficiency 0.9657 0.97 1.5712 0.6992 0.8052 1.3913 0.9401 0.8383 1.0255 1.0637

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1732 907 794 1101 69 1337 2548 128 1801 2244 3342 1229

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 685.05 758.05 1160.09 1073.06 824.91 829.36 1065.49 1159.58 690.41 800.54 1087.97 1082.07
Standard Deviation 89.62 135.98 150.82 181.23 104.45 121.97 148.02 167.59 94.15 132.52 154.05 181.79
Intensity Change Log 2 — 0.146084 0.759956 0.647450 — 0.007762 0.369208 0.491294 — 0.072164 0.559671 0.564240

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000210 0.000850 0.000963 0.000848 0.001200 0.000584 0.000502 0.001542 0.000248 0.000692 0.000612 0.000920
Bud Neck 0.004638 0.009577 0.029617 0.025305 0.000839 0.013195 0.024963 0.043981 0.004493 0.011733 0.026069 0.027250
Bud Site 0.001313 0.001804 0.001895 0.004563 0.000563 0.002250 0.000996 0.009724 0.001284 0.002070 0.001210 0.005100
Cell Periphery 0.000167 0.000106 0.000059 0.000136 0.000097 0.000065 0.000054 0.000186 0.000164 0.000082 0.000055 0.000141
Cytoplasm 0.012959 0.005633 0.000727 0.002613 0.000910 0.001565 0.000508 0.001575 0.012497 0.003209 0.000560 0.002505
Cytoplasmic Foci 0.001590 0.002399 0.000022 0.002351 0.003351 0.000953 0.000007 0.000192 0.001657 0.001537 0.000011 0.002126
Eisosomes 0.000021 0.000032 0.000045 0.000033 0.000065 0.000028 0.000038 0.000046 0.000023 0.000030 0.000040 0.000035
Endoplasmic Reticulum 0.002344 0.004356 0.002142 0.002192 0.004589 0.003583 0.002066 0.002653 0.002430 0.003895 0.002084 0.002240
Endosome 0.000496 0.001126 0.000102 0.002973 0.000973 0.000415 0.000097 0.000610 0.000515 0.000702 0.000098 0.002727
Golgi 0.000158 0.001375 0.000123 0.005795 0.003174 0.000884 0.000064 0.000162 0.000273 0.001083 0.000078 0.005208
Lipid Particles 0.000535 0.000381 0.000045 0.000148 0.001337 0.000445 0.000016 0.000120 0.000566 0.000419 0.000023 0.000146
Mitochondria 0.001336 0.001814 0.001795 0.004082 0.002580 0.003707 0.001020 0.003816 0.001384 0.002942 0.001205 0.004054
Mitotic Spindle 0.001749 0.000714 0.001963 0.015966 0.000225 0.007239 0.001630 0.009776 0.001691 0.004602 0.001709 0.015321
None 0.019659 0.012517 0.001305 0.003692 0.003740 0.003275 0.001209 0.003658 0.019049 0.007010 0.001232 0.003689
Nuclear Periphery 0.000793 0.000357 0.000244 0.000660 0.000090 0.000712 0.000541 0.000824 0.000766 0.000568 0.000470 0.000677
Nuclear Periphery Foci 0.000569 0.000764 0.000034 0.000436 0.000695 0.000405 0.000098 0.000282 0.000574 0.000550 0.000083 0.000420
Nucleolus 0.052887 0.053298 0.035636 0.012775 0.068638 0.049705 0.018812 0.016791 0.053490 0.051157 0.022809 0.013193
Nucleus 0.896977* 0.900567* 0.921730* 0.911536* 0.896032* 0.908571* 0.946623* 0.900516* 0.896941* 0.905336* 0.940709* 0.910388*
Peroxisomes 0.000371 0.001010 0.000726 0.000371 0.009869 0.000948 0.000174 0.000564 0.000735 0.000973 0.000305 0.000391
Vacuole 0.000763 0.000889 0.000634 0.002644 0.000748 0.000623 0.000355 0.002627 0.000763 0.000730 0.000421 0.002643
Vacuole Periphery 0.000465 0.000430 0.000192 0.000883 0.000285 0.000850 0.000227 0.000356 0.000458 0.000680 0.000219 0.000828

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.87 -9.92 -7.96 -2.87 1.71 1.74 1.83 -0.80 -3.97 -4.18 -4.46 -5.38 -8.28 -5.80 -6.13
Bud Neck -5.25 -15.71 -14.66 -10.69 1.66 -10.82 -30.35 -5.23 -3.75 -2.04 -8.82 -29.21 -15.10 -10.33 -1.06
Bud Site -0.94 -3.73 -3.00 -2.56 -1.72 -2.94 -2.73 -2.59 -2.06 -2.40 -1.63 -0.73 -3.67 -2.85 -3.59
Cell Periphery 3.02 5.41 1.67 -0.82 -2.83 0.97 1.32 -0.99 -2.50 -2.93 4.51 6.13 1.59 -2.30 -3.77
Cytoplasm 4.02 9.16 7.89 2.43 -3.26 -1.14 0.50 -1.08 -0.45 -1.51 6.75 9.36 7.88 1.48 -4.25
Cytoplasmic Foci -0.53 4.67 0.84 1.05 -2.48 1.13 1.63 1.52 1.62 -2.00 0.68 4.95 1.19 0.52 -2.55
Eisosomes -4.37 -13.34 -9.20 -2.52 5.56 3.35 2.47 1.34 -5.09 -2.82 -3.51 -12.07 -8.96 -5.66 1.27
Endoplasmic Reticulum -6.47 -1.07 -2.05 5.40 -1.11 1.35 3.22 1.82 1.12 -2.12 -6.15 0.48 -1.87 5.21 -3.62
Endosome -1.51 5.34 -2.35 -1.02 -3.04 0.81 1.26 0.57 -0.69 -2.21 -1.00 5.68 -2.32 -1.87 -3.16
Golgi -2.28 -0.17 -2.50 -1.46 -2.50 0.82 1.12 1.08 1.74 -4.02 -2.40 1.65 -2.40 -1.65 -2.57
Lipid Particles 1.53 5.56 4.21 2.14 -1.84 1.55 2.35 2.13 2.23 -2.77 1.50 6.40 4.63 2.83 -3.03
Mitochondria -1.77 -4.24 -3.51 -2.78 -2.23 -0.69 1.26 -0.90 -0.31 -3.11 -3.08 -0.07 -3.83 -1.01 -3.95
Mitotic Spindle 1.42 -0.82 -4.90 -5.35 -4.46 -4.05 -3.86 -1.94 -0.37 -1.24 -2.33 -1.59 -5.10 -3.97 -4.60
None 2.92 9.33 8.16 4.96 -4.02 0.50 3.62 0.99 0.45 -2.74 6.04 9.43 8.21 3.93 -4.59
Nuclear Periphery 4.13 5.13 -0.91 -5.12 -6.38 -4.29 -3.37 -3.69 -1.09 -1.48 1.64 1.62 -1.34 -2.87 -2.68
Nuclear Periphery Foci -0.63 4.23 1.46 1.81 -2.50 0.83 1.91 1.09 0.57 -1.31 0.33 4.09 1.59 1.45 -2.38
Nucleolus -0.11 3.24 13.93 11.48 8.70 1.74 4.27 4.53 9.39 1.43 0.89 11.36 14.67 15.89 5.98
Nucleus -0.79 -2.06 -1.53 -0.74 0.16 -0.68 -2.19 -0.07 0.71 2.52 -1.83 -8.04 -1.44 0.00 4.75
Peroxisomes -3.65 -4.93 0.14 3.78 5.42 1.64 1.78 1.71 1.32 -4.41 -0.71 1.94 1.60 3.20 -1.68
Vacuole -0.32 -1.19 -3.55 -3.37 -3.13 0.41 0.84 -1.82 -1.89 -1.94 0.33 0.92 -3.97 -4.17 -4.35
Vacuole Periphery 0.24 2.37 -0.82 -1.01 -2.67 -1.40 0.54 -0.71 1.04 -1.24 -0.88 2.01 -0.81 0.33 -2.53
Standard name
Human Ortholog
Description mRNA 3' end processing factor; essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (96%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Pcf11

Pcf11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pcf11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available