Standard name
Human Ortholog
Description Phospholipid-binding protein that interacts with both Ypt7p and Vps33p; may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0.09 0.15 0.09 0.12 0.1 0.11 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.1 0.1 0.07 0.06 0.11 0.09 0.12 0.11 0.14 0 0 0 0.08 0.05 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.17 0.26 0.45 0.41 0.4 0.25 0.16 0.24 0.21 0.14 0.2 0.14 0.44 0.38 0.38 0.56 0.55 0.54 0.36 0.4 0.49 0.46 0.45 0.49
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.29 0.17 0.18 0.32 0.21 0.36 0.39 0.36 0.38 0.46 0.43 0.48 0 0 0 0.06 0.07 0.05 0 0 0 0 0 0
Nucleus 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0.05
Nucleolus 0.35 0.34 0.18 0.17 0.19 0.16 0.15 0.15 0.17 0.13 0.15 0.14 0.25 0.19 0.19 0.36 0.41 0.45 0 0 0 0 0 0
Peroxisomes 0 0 0.09 0.07 0.09 0.12 0.13 0.11 0.09 0.06 0.06 0 0.08 0.1 0.08 0 0 0.05 0 0 0 0 0 0
SpindlePole 0.18 0.08 0.33 0.25 0.38 0.31 0.34 0.29 0.36 0.23 0.26 0.19 0.5 0.48 0.4 0.44 0.47 0.44 0.13 0.09 0.12 0.12 0.09 0.1
Vac/Vac Membrane 0.44 0.48 0.3 0.23 0.12 0.09 0.1 0.12 0.09 0.14 0.15 0.14 0.31 0.36 0.4 0.27 0.2 0.23 0.4 0.4 0.22 0.33 0.35 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 3 2 1 0 0 0 1 0 0 0 0 1 0 0 0 5 0 0 1
Bud 3 1 14 3 16 38 71 48 52 56 39 30 2 0 3 2 5 1 2 1 4 0 6 4
Bud Neck 0 0 1 0 1 3 3 0 2 4 1 1 0 0 1 0 0 0 0 0 0 1 0 1
Bud Site 0 0 0 0 1 0 1 1 3 5 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 0 1 2 1 2 5 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 2 21 21 32 31 31 56 37 67 39 67 11 17 19 19 14 19 0 2 2 1 2 1
Endoplasmic Reticulum 0 0 0 0 0 3 0 2 2 7 1 3 0 0 0 6 7 1 0 0 0 0 0 0
Endosome 37 34 215 87 128 111 78 129 90 79 71 68 208 187 207 131 164 135 84 60 243 167 230 328
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 4 0 1 1 4 2 4 7
Mitochondria 63 23 87 68 67 157 189 188 160 252 153 231 2 0 10 14 22 12 5 1 8 6 2 6
Nucleus 4 6 2 0 2 5 2 6 1 4 2 2 6 5 9 3 3 4 1 2 1 5 11 8
Nuclear Periphery 7 5 9 4 3 10 3 4 3 4 2 6 3 4 7 4 6 3 7 1 43 7 20 35
Nucleolus 75 45 88 36 61 69 73 77 70 70 55 66 121 91 107 85 123 111 3 1 1 5 8 12
Peroxisomes 2 0 45 14 30 54 61 58 36 30 20 20 37 47 46 10 13 13 0 0 5 0 0 1
SpindlePole 39 10 156 54 122 134 162 153 150 127 92 89 239 233 223 102 142 110 31 13 56 43 43 70
Vac/Vac Membrane 95 63 145 50 38 39 46 61 40 78 54 67 150 177 222 63 60 57 94 60 109 119 177 188
Unique Cell Count 214 132 477 213 317 438 481 527 422 545 355 477 477 487 551 234 299 248 237 150 493 364 512 672
Labelled Cell Count 331 189 784 337 501 658 722 785 648 784 533 656 779 761 856 439 564 466 237 150 493 364 512 672


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 8.7 7.3 6.7 6.8 6.2 6.3 6.5 6.2 6.1 5.7 6.0 9.7 10.1 9.4 10.4 10.7 10.3 9.1 9.8 10.5
Std Deviation (1e-4) 1.4 1.8 1.3 1.7 1.6 1.4 1.5 1.8 1.6 1.7 1.5 1.7 1.6 1.7 1.7 2.6 2.3 2.6 1.5 1.7 1.7
Intensity Change (Log2) – – – -0.13 -0.09 -0.24 -0.21 -0.18 -0.24 -0.25 -0.36 -0.29 0.4 0.47 0.37 0.5 0.55 0.5 0.32 0.42 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -1.2 1.5 3.7 6.4 4.1 5.4 4.6 4.7 2.5 -3.0 -3.8 -3.0 -1.8 -1.1 -2.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.8 3.1 1.7 1.4 3.7 2.7 4.5 3.6 5.1 -1.8 -0.7 -0.8 2.0 0.2 1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.0 -1.3 -6.2 -9.7 -6.9 -7.5 -10.8 -7.5 -10.4 -0.5 -2.1 -2.4 2.7 2.7 2.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.0 1.0 6.0 7.2 6.2 6.6 9.5 7.8 9.9 -9.5 -9.9 -9.0 -4.4 -4.2 -5.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 1.9 0 0 0
Nuclear Periphery 0 0 0.4 -1.8 -1.6 -1.5 -1.6 0 -0.8 -1.7 -1.4 -0.8 0 0.1 0
Nucleolus -0.5 0.3 -1.1 -1.4 -1.6 -0.7 -2.5 -1.1 -1.9 2.6 0.1 0.4 5.2 6.9 7.5
Peroxisomes -1.2 0 1.4 1.6 0.8 -0.5 -2.4 -2.0 -3.2 -0.9 0.1 -0.6 -2.4 -2.6 -2.0
SpindlePole -1.9 1.7 -0.7 0.3 -1.3 0.9 -3.4 -2.1 -5.0 5.5 4.8 2.6 2.8 4.1 3.1
Vacuole -1.9 -6.0 -8.1 -8.1 -7.4 -7.7 -6.2 -5.1 -6.1 0.4 2.0 3.3 -1.0 -3.2 -2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.1507 6.1456 6.0708 6.015 5.5297 5.9809 5.0766 5.8284 5.2419 5.0968 4.6029 5.3281 4.034 4.6292 4.279 4.1283 4.822 4.4261
Actin 0.0118 0.0001 0.0001 0 0.0006 0.0002 0.0023 0.0003 0.0099 0 0.0184 0.0002 0.0116 0.0007 0.0096 0 0 0.0001
Bud 0.0001 0.0005 0.0002 0.0003 0.0008 0.0008 0.0003 0.0002 0.0002 0 0.0001 0.0001 0.0004 0.0003 0.0002 0 0 0.0002
Bud Neck 0.0005 0.0013 0.0003 0.0003 0.0002 0.001 0.0009 0.0005 0.0017 0.0002 0.0004 0.0004 0.0012 0.0004 0.0006 0.0002 0.0001 0.0004
Bud Periphery 0.0003 0.0006 0.0003 0.0002 0.003 0.0012 0.0006 0.0001 0.0003 0.0001 0.0003 0.0001 0.0007 0.0002 0.0004 0.0001 0.0001 0.0013
Bud Site 0.0004 0.0015 0.0005 0.0005 0.0008 0.0011 0.0016 0.0018 0.003 0.0001 0.0004 0.0003 0.0027 0.0007 0.0005 0.0002 0 0.0002
Cell Periphery 0.0003 0.0005 0.0004 0.0002 0.0002 0.0004 0.0005 0.0004 0.0004 0.0001 0.0002 0.0002 0.0007 0.0004 0.0008 0.0001 0.0002 0.0004
Cytoplasm 0.0021 0.0022 0.0009 0.0022 0.0099 0.0008 0.0015 0.0025 0.0009 0.0006 0.0022 0.0006 0.0018 0.002 0.0007 0.0006 0.0003 0.0003
Cytoplasmic Foci 0.1288 0.142 0.1727 0.1184 0.1051 0.0924 0.1442 0.1888 0.1435 0.0749 0.1032 0.091 0.1375 0.1547 0.1262 0.1466 0.064 0.0763
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0013 0.0028 0.0004 0.0003 0.0009 0.0006 0.0018 0.0005 0.0005 0.0003 0.0021 0.0006 0.0018 0.0003 0.0005 0.0002 0.0004 0.0006
Endosome 0.3394 0.3359 0.3485 0.5201 0.4345 0.3746 0.366 0.337 0.3727 0.5023 0.4937 0.3466 0.2631 0.2785 0.2632 0.3641 0.4288 0.2287
Golgi 0.0311 0.0228 0.0452 0.0247 0.0313 0.0339 0.0357 0.0259 0.041 0.029 0.0397 0.0331 0.032 0.0241 0.0303 0.0445 0.029 0.0388
Lipid Particles 0.2316 0.157 0.2241 0.0867 0.176 0.2072 0.2007 0.185 0.1507 0.089 0.1542 0.2367 0.2462 0.2301 0.2692 0.1609 0.165 0.3686
Mitochondria 0.0064 0.0114 0.0032 0.0017 0.0295 0.0055 0.0071 0.0014 0.0142 0.0257 0.0203 0.0041 0.0092 0.0033 0.0094 0.0011 0.0101 0.0115
None 0.0021 0.0002 0.0001 0.0002 0.0132 0.0001 0.0005 0.0001 0.0002 0.0001 0.0009 0.0001 0.0026 0.0001 0.0001 0 0 0.0001
Nuclear Periphery 0.0106 0.0183 0.0017 0.0023 0.0047 0.0074 0.0044 0.0053 0.0069 0.0009 0.0098 0.0072 0.013 0.0051 0.0018 0.0007 0.0012 0.008
Nucleolus 0.0077 0.0042 0.005 0.0026 0.0012 0.0061 0.0087 0.0036 0.0056 0.0005 0.0008 0.0033 0.0048 0.0027 0.0108 0.0003 0.0004 0.0046
Nucleus 0.0041 0.0013 0.0036 0.0008 0.009 0.0012 0.002 0.0008 0.001 0.0003 0.0011 0.0007 0.0035 0.0009 0.0017 0.0002 0.0003 0.0012
Peroxisomes 0.0268 0.0123 0.0248 0.0081 0.0126 0.0126 0.0169 0.0181 0.0254 0.0049 0.0107 0.0208 0.0227 0.0097 0.0187 0.0132 0.0017 0.0046
Punctate Nuclear 0.0084 0.0055 0.0025 0.0003 0.0248 0.0033 0.0048 0.0025 0.0011 0.0002 0.0018 0.002 0.0116 0.0033 0.0007 0.0004 0.0002 0.0015
Vacuole 0.1063 0.1793 0.0942 0.1355 0.0676 0.1285 0.1166 0.1287 0.1188 0.1678 0.0468 0.1032 0.1232 0.1454 0.1491 0.1604 0.1404 0.0887
Vacuole Periphery 0.0799 0.1002 0.0711 0.0946 0.0741 0.1212 0.0829 0.0963 0.102 0.103 0.0927 0.1486 0.1095 0.1372 0.1054 0.1062 0.1577 0.1638

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.7216 6.5179 5.374 15.1754 11.0289 9.7162 12.9974 18.039 15.7367 16.0654
Translational Efficiency 1.3968 2.3415 2.5329 1.1878 1.4589 1.8968 1.3889 1.1063 1.2614 1.2743

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Phospholipid-binding protein that interacts with both Ypt7p and Vps33p; may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase
Localization
Cell Percentages vacuolar membrane (57%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-1

Ivy1

Ivy1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ivy1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available