Standard name
Human Ortholog
Description U1 snRNP protein involved in splicing; required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0.06 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0 0.06 0.09 0.05 0.05 0.05 – 0.05 0 0.05 0 0 0 0.06 0.06 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.07 0.31 0.31 0.33 0.63 0.71 0.67 – 0.67 0.76 0.77 0 0 0 0 0.07 0 0 0 0 0 0 0
Nucleus 0.91 0.91 0.92 0.85 0.83 0.76 0.71 0.69 – 0.66 0.67 0.64 0.91 0.92 0.93 0.9 0.88 0.87 0.82 0.78 0.75 0.82 0.78 0.72
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0.13 0 0 0 0 0 0.07 – 0.05 0.05 0 0 0 0 0 0 0 0.08 0.07 0.13 0.06 0.08 0.09
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.05 0 0.08 0.06 0 0.07 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 1 1
Bud 0 0 0 0 1 7 5 12 – 2 6 12 0 0 0 0 0 1 3 1 1 2 3 2
Bud Neck 0 0 0 0 0 0 1 1 – 0 0 0 2 0 0 0 0 0 0 0 1 0 1 1
Bud Site 0 0 0 1 1 15 15 11 – 32 25 24 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 8 3 16 29 21 31 44 – 27 19 28 10 10 6 12 15 8 1 0 0 0 2 0
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 – 0 1 0 3 2 1 2 6 3 0 0 0 2 0 3
Endosome 1 0 0 1 1 0 0 1 – 1 0 0 1 3 2 3 4 0 2 2 4 3 3 2
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 1 1 0 2 0 0 0 2 2
Mitochondria 10 9 73 89 106 277 422 557 – 341 391 438 8 6 13 7 17 3 2 2 1 0 2 0
Nucleus 197 109 215 241 269 334 421 573 – 336 347 364 243 254 309 175 223 133 183 102 180 148 240 241
Nuclear Periphery 0 0 2 3 0 3 2 2 – 5 1 2 0 0 0 0 0 0 1 0 0 0 1 1
Nucleolus 20 16 5 2 3 8 16 56 – 24 27 23 1 4 1 2 4 3 18 9 31 11 24 31
Peroxisomes 1 0 0 0 0 0 0 0 – 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1
SpindlePole 0 0 3 0 0 6 7 10 – 9 1 6 10 6 6 4 2 3 0 1 2 3 2 7
Vac/Vac Membrane 3 3 3 3 6 3 4 4 – 3 7 3 2 1 4 5 13 8 7 10 14 6 21 35
Unique Cell Count 216 120 233 284 326 438 595 827 509 515 570 268 276 331 194 254 153 224 132 240 181 310 334
Labelled Cell Count 236 146 304 356 416 674 924 1271 780 825 900 282 286 343 212 285 162 224 132 240 181 310 334


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.2 4.3 4.3 4.2 3.7 3.2 3.4 – 3.4 3.0 3.1 6.1 6.0 5.9 6.7 6.6 6.9 7.1 7.4 7.7
Std Deviation (1e-4) 1.4 1.2 0.8 1.3 1.5 0.9 1.0 1.2 – 5.5 0.9 0.8 1.1 1.2 0.9 1.2 1.5 1.3 1.7 1.5 1.7
Intensity Change (Log2) – – – -0.01 -0.04 -0.21 -0.42 -0.33 – -0.35 -0.5 -0.5 0.5 0.48 0.45 0.64 0.61 0.68 0.73 0.77 0.84


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 2.6 3.8 2.3 2.6 2.6 – 2.6 1.8 2.4 1.7 1.7 0 2.7 2.7 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 -8.6 -9.1 -8.9 -7.3 -7.0 -7.1
Nucleus -2.6 -3.3 -5.1 -6.6 -7.1 – -7.6 -7.3 -8.1 -0.6 -0.1 0.5 -0.8 -1.6 -1.7
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 1.7 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 2.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0264 0 0.0124 0.0108 0.0158 0.0002 0.0071 0 0.0042 0 0 0.0038 0.0565 0 0.0002 0 0.0035 0.0003
Bud 0.0002 0.0002 0.0002 0.0001 0.0001 0 0.0001 0 0.0001 0 0 0 0.001 0.0001 0.0005 0 0.0009 0.0001
Bud Neck 0.0011 0.0002 0.0005 0.0002 0.0004 0.0008 0.0008 0.0003 0.0007 0.0001 0.0012 0.0012 0.0011 0.0002 0.0004 0.0001 0.0081 0.0043
Bud Periphery 0.0004 0.0002 0.0002 0.0002 0.0002 0 0.0001 0 0.0002 0 0 0.0001 0.0009 0.0001 0.0014 0 0.0006 0.0002
Bud Site 0.0019 0.0003 0.0026 0.0002 0.0001 0.0001 0.0004 0.0001 0.0007 0 0.0001 0 0.0067 0.0003 0.0003 0.0001 0.0069 0.0007
Cell Periphery 0.0001 0 0.0001 0.0001 0 0 0 0 0.0002 0 0 0 0.0004 0 0.0002 0 0.0002 0.0001
Cytoplasm 0.0033 0.0009 0.0008 0.0002 0.0012 0.0004 0.0008 0.0003 0.0004 0.0007 0.0005 0.0009 0.1174 0.0001 0.0014 0.0002 0.0276 0.0271
Cytoplasmic Foci 0.0141 0 0.0021 0.0037 0.0008 0.0002 0.001 0 0.0001 0 0 0.0001 0.0315 0 0 0 0.1297 0.0003
Eisosomes 0.0002 0 0.0001 0 0 0 0.0001 0 0.0005 0 0 0 0.0003 0 0 0 0.0003 0
Endoplasmic Reticulum 0.0014 0.0001 0.0004 0.0002 0.0025 0.0002 0.0002 0 0.0003 0 0 0.0001 0.0186 0 0.0003 0 0.0017 0.0004
Endosome 0.0136 0.0006 0.0019 0.004 0.0093 0.0008 0.0013 0 0.0009 0 0.0001 0.0002 0.0242 0 0.0001 0 0.1413 0.0007
Golgi 0.0054 0 0.0016 0.003 0.0027 0 0.0009 0 0.0004 0 0 0.0002 0.0319 0 0 0 0.0343 0
Lipid Particles 0.0119 0 0.0007 0.0211 0.0005 0.0001 0.0018 0 0.0005 0 0 0.0001 0.0156 0 0 0 0.0043 0.0001
Mitochondria 0.0021 0.0004 0.0009 0.0034 0.0013 0.0003 0.0003 0.0001 0.0006 0.0001 0.0002 0.0001 0.0112 0.0004 0.0017 0.0005 0.0047 0.0004
None 0.0038 0.0001 0.0003 0.0001 0.0005 0.0002 0.0025 0.0003 0.0064 0.0012 0.0005 0.0028 0.0127 0 0.0009 0.0001 0.0607 0.0109
Nuclear Periphery 0.0111 0.0048 0.0045 0.0012 0.0067 0.0036 0.0073 0.0012 0.006 0.0018 0.0032 0.0019 0.0123 0.0005 0.0057 0.0012 0.0077 0.0052
Nucleolus 0.0239 0.0167 0.0156 0.0141 0.0285 0.0422 0.0152 0.0156 0.0088 0.011 0.0361 0.0452 0.013 0.0164 0.0171 0.0095 0.0671 0.0717
Nucleus 0.8505 0.9689 0.936 0.918 0.9262 0.9369 0.9518 0.9548 0.9602 0.9681 0.94 0.9315 0.6126 0.9812 0.9657 0.9878 0.3555 0.859
Peroxisomes 0.0154 0 0.0035 0.0149 0.0003 0.0001 0.0014 0 0.0001 0 0 0 0.0226 0 0 0 0.0047 0
Punctate Nuclear 0.0108 0.004 0.0153 0.0035 0.0022 0.0135 0.0066 0.0272 0.0088 0.0169 0.0178 0.0117 0.0039 0.0003 0.0015 0.0004 0.1348 0.0176
Vacuole 0.0016 0.0021 0.0002 0.0007 0.0002 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0047 0.0001 0.0015 0.0001 0.0041 0.0006
Vacuole Periphery 0.0008 0.0005 0.0002 0.0004 0.0004 0.0001 0.0001 0 0.0001 0 0 0 0.0011 0.0001 0.0011 0 0.0013 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.1353 13.2723 7.6103 11.2773 13.5624 8.0939 9.6072 13.1743 12.0346 14.8181
Translational Efficiency 0.7417 0.5782 0.7215 0.5635 0.5457 1.0571 1.0721 0.4903 0.5418 0.5515

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2011 1673 2540 1152 2016 891 92 1663 4027 2564 2632 2815

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 744.39 840.13 920.27 983.29 755.13 805.97 1065.76 995.99 749.77 828.26 925.36 990.79
Standard Deviation 93.43 123.63 134.40 151.71 110.01 113.02 128.47 138.57 102.21 121.15 136.83 144.23
Intensity Change Log 2 — 0.174554 0.305999 0.401558 — 0.094001 0.497086 0.399406 — 0.134551 0.405387 0.400475

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000131 0.000537 0.000622 0.001651 0.000252 0.000569 0.001615 0.001081 0.000192 0.000548 0.000657 0.001314
Bud Neck 0.002876 0.006441 0.027981 0.029917 0.004579 0.007674 0.009424 0.025069 0.003728 0.006870 0.027333 0.027053
Bud Site 0.000404 0.000924 0.001756 0.011860 0.000874 0.001735 0.001242 0.006073 0.000639 0.001206 0.001738 0.008441
Cell Periphery 0.000054 0.000082 0.000072 0.000360 0.000116 0.000088 0.000048 0.000123 0.000085 0.000084 0.000072 0.000220
Cytoplasm 0.005900 0.003953 0.002321 0.012971 0.013117 0.007116 0.000374 0.010452 0.009513 0.005052 0.002253 0.011483
Cytoplasmic Foci 0.001167 0.000864 0.000016 0.004453 0.000900 0.002442 0.000037 0.002906 0.001033 0.001412 0.000017 0.003539
Eisosomes 0.000013 0.000023 0.000029 0.000046 0.000016 0.000030 0.000051 0.000031 0.000015 0.000026 0.000030 0.000037
Endoplasmic Reticulum 0.001478 0.003903 0.003200 0.007446 0.002450 0.004053 0.006928 0.005865 0.001965 0.003955 0.003330 0.006512
Endosome 0.000233 0.000690 0.000185 0.006343 0.000312 0.000896 0.000195 0.003236 0.000272 0.000762 0.000185 0.004507
Golgi 0.000227 0.000431 0.000086 0.005167 0.000283 0.001757 0.000107 0.004079 0.000255 0.000891 0.000086 0.004524
Lipid Particles 0.000425 0.000870 0.000058 0.001154 0.000437 0.001030 0.000177 0.001184 0.000431 0.000926 0.000062 0.001172
Mitochondria 0.001047 0.003521 0.001429 0.008893 0.001257 0.004411 0.001369 0.004504 0.001152 0.003830 0.001427 0.006300
Mitotic Spindle 0.002073 0.002942 0.003309 0.037055 0.000474 0.010902 0.000381 0.017321 0.001273 0.005708 0.003207 0.025397
None 0.008800 0.005771 0.002736 0.006354 0.012409 0.005355 0.001458 0.005597 0.010606 0.005627 0.002691 0.005907
Nuclear Periphery 0.000490 0.001071 0.000785 0.002729 0.000599 0.001475 0.000275 0.003039 0.000545 0.001211 0.000768 0.002912
Nuclear Periphery Foci 0.000469 0.000468 0.000136 0.001042 0.000624 0.000752 0.000127 0.001167 0.000546 0.000567 0.000136 0.001116
Nucleolus 0.030768 0.035375 0.018023 0.014774 0.027737 0.032016 0.020841 0.011984 0.029251 0.034208 0.018122 0.013126
Nucleus 0.942739* 0.930498* 0.936192* 0.828986* 0.932708* 0.912979* 0.952986* 0.888544* 0.937717* 0.924410* 0.936779* 0.864171*
Peroxisomes 0.000340 0.000656 0.000153 0.000641 0.000290 0.002815 0.000667 0.000499 0.000315 0.001406 0.000171 0.000557
Vacuole 0.000245 0.000610 0.000591 0.014901 0.000410 0.001484 0.001546 0.005567 0.000327 0.000914 0.000624 0.009387
Vacuole Periphery 0.000121 0.000370 0.000319 0.003257 0.000156 0.000419 0.000149 0.001681 0.000139 0.000387 0.000313 0.002326

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.27 -24.45 -16.85 -10.64 -10.76 -3.87 -9.04 -10.28 -5.84 3.93 -5.89 -16.50 -19.08 -11.31 -10.58
Bud Neck -6.14 -27.24 -18.21 -15.42 -1.24 -3.58 -4.63 -17.37 -12.48 -5.98 -6.38 -26.67 -25.26 -20.44 0.25
Bud Site -2.68 -7.17 -8.06 -7.54 -6.56 -1.95 -2.76 -6.04 -3.91 -3.28 -2.60 -5.70 -10.15 -8.88 -7.77
Cell Periphery -2.33 -2.05 -2.77 -2.27 -2.50 1.41 2.36 0.29 -1.44 -2.59 -0.11 1.66 -2.77 -2.70 -3.37
Cytoplasm 1.81 4.28 -2.83 -4.02 -5.54 2.80 9.45 1.68 -1.29 -7.61 4.10 8.65 -0.63 -4.28 -8.00
Cytoplasmic Foci 0.88 3.22 -0.77 -1.53 -3.15 -2.54 4.61 -1.32 1.32 -3.44 -1.11 4.96 -1.35 -0.45 -4.72
Eisosomes -7.43 -16.36 -11.74 -8.39 -6.40 -6.65 -7.99 -12.76 -1.68 4.27 -9.53 -17.45 -17.06 -8.77 -6.30
Endoplasmic Reticulum -7.67 -13.90 -16.13 -9.43 -11.66 -5.40 -6.92 -13.47 -5.27 1.34 -8.66 -12.13 -21.41 -10.60 -14.40
Endosome -2.19 0.70 -4.55 -3.68 -4.61 -2.64 -0.89 -3.95 -2.26 -3.48 -3.18 1.84 -6.03 -4.33 -6.20
Golgi -1.07 1.12 -2.80 -2.51 -3.04 -1.57 0.64 -2.38 -0.34 -2.66 -1.77 1.36 -3.52 -2.07 -3.94
Lipid Particles -1.92 4.48 -1.60 0.34 -3.79 -3.38 1.25 -1.26 1.10 -1.95 -3.18 5.44 -1.88 0.80 -4.30
Mitochondria -3.14 -2.63 -4.85 -1.90 -4.17 -3.11 -1.00 -3.79 0.66 -4.12 -4.21 -2.09 -6.32 -1.02 -5.64
Mitotic Spindle -0.98 -2.55 -7.95 -7.61 -6.98 -3.76 -1.34 -7.44 -1.75 -2.77 -3.90 -3.73 -10.75 -7.57 -8.04
None 2.53 6.31 3.38 0.58 -4.53 4.81 9.85 6.42 0.42 -6.02 5.32 10.72 7.01 0.80 -5.89
Nuclear Periphery -2.94 -2.19 -6.68 -4.61 -5.09 -4.93 -0.34 -3.69 -1.89 -3.40 -4.78 -1.95 -6.18 -4.01 -4.76
Nuclear Periphery Foci 0.29 2.50 -2.29 -2.87 -4.58 -0.61 -0.47 -1.31 -0.46 0.17 -0.00 3.80 -2.57 -2.72 -6.33
Nucleolus -1.99 4.32 7.65 9.38 4.24 -1.57 2.15 8.88 7.55 2.10 -2.88 4.48 11.90 12.95 6.48
Nucleus 2.88 5.24 13.40 11.54 11.16 3.51 -0.18 7.96 2.82 4.36 4.09 4.58 15.15 10.80 11.86
Peroxisomes -2.95 2.20 -1.52 1.88 -5.10 -2.57 -3.25 -1.34 2.44 2.10 -3.20 2.44 -1.87 2.83 -4.87
Vacuole -3.22 -5.36 -8.54 -8.33 -8.29 -3.71 -1.86 -8.11 -5.91 -0.86 -4.68 -4.60 -11.52 -10.63 -10.85
Vacuole Periphery -2.02 -1.10 -2.84 -2.25 -2.40 -2.83 -1.44 -2.60 -1.67 -2.47 -2.91 -1.00 -3.86 -2.80 -2.99
Standard name
Human Ortholog
Description U1 snRNP protein involved in splicing; required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Prp42

Prp42


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp42-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available