ORF
Human Ortholog
Description Protein of unknown function; may interact with ribosomes, based on co-purification experiments
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.07
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0 0.09 0.06 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.06 0 0.06 0.09 0 0 0 0 0 – 0 0 0.08 0.07 0.07 0.11 0.19 0.12 0 0 0 0 0 0
Cytoplasm 0.88 0.94 0.98 0.99 0.96 0.96 0.92 0.93 0.81 – 0.82 0.84 0.99 0.98 0.99 0.94 0.95 0.92 0.75 0.83 0.86 0.59 0.61 0.69
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.14 0.09 0.31 – 0.37 0.27 0 0 0 0 0 0 0 0 0 0.08 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 1 1 0 0 0 0 2 0 – 3 1 0 2 1 9 3 8 3 4 3 8 27 28
Bud 4 4 4 2 2 1 1 5 7 – 5 1 4 2 2 3 2 1 2 2 1 9 20 16
Bud Neck 0 0 2 0 0 0 2 2 0 – 0 0 0 0 0 0 0 0 7 6 2 14 23 12
Bud Site 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 7 10 14 11 7 12 5 5 – 5 3 28 30 30 86 88 61 3 1 2 2 14 12
Cytoplasm 89 156 172 147 272 198 307 276 198 – 114 160 349 400 428 704 447 473 83 147 152 94 226 268
Endoplasmic Reticulum 3 3 0 0 4 0 2 0 0 – 0 3 2 2 1 27 10 20 2 0 0 2 6 8
Endosome 0 0 0 0 0 1 0 1 0 – 0 0 1 2 1 2 0 1 0 2 1 1 5 3
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 1 0 0 1 0 0 2 0
Mitochondria 2 6 1 1 2 20 47 28 75 – 51 52 3 1 1 7 2 2 0 3 7 12 18 14
Nucleus 1 0 0 0 1 1 0 3 1 – 1 0 0 0 1 12 8 10 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 2 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 2 1 1 2 0 – 0 2 0 3 0 3 2 0 0 1 1 0 2 3
Vac/Vac Membrane 0 0 0 0 1 2 3 3 4 – 1 3 1 0 0 28 6 20 3 5 3 10 17 14
Unique Cell Count 101 166 175 149 282 207 332 296 245 139 191 353 407 434 752 469 516 112 178 176 160 372 389
Labelled Cell Count 110 178 191 164 295 231 375 327 292 180 226 389 443 466 883 570 596 112 178 176 160 372 389


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.3 5.8 5.5 6.3 4.8 5.2 5.4 4.3 – 4.0 4.5 5.7 5.9 6.0 10.2 10.5 10.4 5.3 5.3 5.8
Std Deviation (1e-4) 0.5 1.0 0.8 1.0 1.4 0.9 1.2 1.4 0.9 – 1.0 1.2 1.0 1.0 1.1 1.9 2.2 1.9 1.2 1.1 1.4
Intensity Change (Log2) – – – -0.08 0.11 -0.28 -0.16 -0.11 -0.45 – -0.55 -0.36 -0.04 0.01 0.04 0.8 0.85 0.83 -0.13 -0.14 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 1.3 -0.9 0 -1.1 -2.4 0 – 0 0 0.9 0.7 0.5 2.2 4.1 2.3
Cytoplasm 0.3 -1.1 -1.5 -2.7 -2.5 -5.5 – -5.0 -4.8 0.5 0 0.3 -2.4 -1.7 -3.0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 2.5 0 2.6
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 5.0 3.9 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 2.6 0 2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3171 3.3484 2.9451 2.4199 2.7293 2.9968 0.7049 0.9884 1.1474 0.7357 0.2918 0.9543 1.2527 2.0868 1.742 1.3321 1.4815 1.6786
Actin 0.0248 0.0011 0.0178 0.0035 0.0554 0.005 0.0113 0.0079 0.0288 0.0143 0.0414 0.0194 0.0159 0.01 0.0092 0.0009 0.0033 0.0235
Bud 0.0006 0.0005 0.0071 0.0039 0.0011 0.0002 0.0045 0.0045 0.0104 0.007 0.0007 0.0009 0.0025 0.0023 0.0024 0.0002 0.0117 0.0009
Bud Neck 0.0032 0.0008 0.0095 0.0028 0.0307 0.0229 0.0111 0.0015 0.01 0.0018 0.0011 0.0679 0.0019 0.0006 0.001 0.0012 0.006 0.119
Bud Periphery 0.0012 0.0011 0.0096 0.0323 0.0032 0.0007 0.0096 0.0047 0.024 0.0234 0.0013 0.0024 0.003 0.001 0.0037 0.0002 0.0083 0.0017
Bud Site 0.0079 0.0182 0.0228 0.0018 0.0153 0.0008 0.0306 0.0519 0.0351 0.0098 0.0007 0.001 0.0192 0.0329 0.0089 0.0001 0.0046 0.0017
Cell Periphery 0.0127 0.0115 0.0088 0.0159 0.0242 0.0045 0.0032 0.0028 0.0021 0.0021 0.001 0.0012 0.0042 0.0023 0.0012 0.0003 0.0012 0.0015
Cytoplasm 0.5388 0.5948 0.6242 0.619 0.4786 0.7054 0.6731 0.7787 0.7209 0.8063 0.7186 0.7763 0.7399 0.8018 0.79 0.7926 0.8375 0.7298
Cytoplasmic Foci 0.0378 0.0178 0.0114 0.0089 0.0098 0.0047 0.0297 0.0088 0.023 0.0193 0.0293 0.0145 0.0051 0.0048 0.0105 0.0022 0.0095 0.0095
Eisosomes 0.0022 0.0008 0.0008 0.0002 0.0016 0.0004 0.0003 0.0001 0.0002 0.0002 0.0002 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0054 0.0019 0.0038 0.0018 0.0031 0.0024 0.0104 0.0035 0.0033 0.005 0.0036 0.0021 0.0039 0.0027 0.0027 0.001 0.0041 0.0021
Endosome 0.0173 0.0032 0.007 0.0028 0.0305 0.0021 0.0449 0.003 0.0214 0.0059 0.02 0.0112 0.0049 0.0012 0.0075 0.0003 0.012 0.0133
Golgi 0.0123 0.0009 0.0027 0.0007 0.0104 0.0017 0.0196 0.0007 0.0077 0.002 0.008 0.0023 0.0012 0.0005 0.0033 0.0001 0.0035 0.0087
Lipid Particles 0.0153 0.0047 0.0021 0.0003 0.0357 0.0031 0.0177 0.0009 0.0035 0.0009 0.0193 0.0025 0.0015 0.0003 0.0022 0.0001 0.0042 0.0054
Mitochondria 0.0256 0.0003 0.0043 0.0009 0.0183 0.0062 0.0152 0.0003 0.0043 0.0015 0.0013 0.0016 0.0011 0.0002 0.0009 0.0001 0.0038 0.0039
None 0.2537 0.3333 0.2519 0.3001 0.2584 0.2326 0.0885 0.1187 0.0948 0.0764 0.1064 0.0861 0.186 0.1352 0.149 0.1992 0.0802 0.0673
Nuclear Periphery 0.0011 0.0002 0.0018 0.0003 0.0061 0.0011 0.0053 0.0005 0.0008 0.0009 0.0005 0.0014 0.0031 0.0004 0.0005 0.0001 0.0005 0.0013
Nucleolus 0.0008 0.0003 0.0012 0.0001 0.0013 0.0001 0.0011 0.0061 0.0002 0.0005 0.0001 0.0001 0.0003 0.0001 0.0002 0 0.0003 0.0001
Nucleus 0.001 0.0007 0.0026 0.0007 0.0016 0.0005 0.0023 0.0018 0.0014 0.0018 0.0007 0.0021 0.0011 0.0013 0.0008 0.0005 0.001 0.0007
Peroxisomes 0.0285 0.0038 0.0043 0.0002 0.0059 0.0039 0.0078 0.0003 0.0043 0.009 0.0428 0.0041 0.0011 0.0007 0.0031 0.0001 0.0021 0.0066
Punctate Nuclear 0.0068 0.0025 0.0026 0.0002 0.0031 0.0002 0.0015 0.0008 0.0009 0.0027 0.0007 0.0015 0.0013 0.0003 0.0009 0.0001 0.0004 0.0012
Vacuole 0.0025 0.0015 0.0034 0.0033 0.0035 0.0011 0.0098 0.0022 0.0026 0.0083 0.002 0.001 0.0021 0.0012 0.0016 0.0006 0.0049 0.001
Vacuole Periphery 0.0004 0 0.0003 0.0003 0.0019 0.0003 0.0027 0.0001 0.0004 0.0007 0.0003 0.0002 0.0005 0.0001 0.0002 0 0.0006 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.0691 7.5105 10.8366 9.7286 11.1956 11.6624 12.4455 9.6812 11.5933 11.8367
Translational Efficiency 1.1749 1.5921 0.9739 0.9676 1.017 1.0573 0.9778 1.0941 0.8233 1.0328

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1893 1045 709 1625 1339 1531 979 1800 3232 2576 1688 3425

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 726.71 743.22 960.98 939.87 754.61 820.67 864.87 928.47 738.27 789.25 905.24 933.88
Standard Deviation 81.53 114.69 136.24 115.25 85.03 103.55 109.59 111.44 84.13 114.70 130.43 113.41
Intensity Change Log 2 — 0.032410 0.403127 0.371081 — 0.121071 0.196752 0.299124 — 0.078256 0.301685 0.334874

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001052 0.001733 0.003469 0.007906 0.000635 0.002060 0.000862 0.007756 0.000879 0.001928 0.001957 0.007827
Bud Neck 0.054666 0.028289 0.032532 0.020626 0.031966 0.050706 0.026285 0.022549 0.045261 0.041612 0.028909 0.021637
Bud Site 0.028610 0.052143 0.070428 0.139871* 0.020065 0.049633 0.052667 0.122955* 0.025070 0.050651 0.060127 0.130981*
Cell Periphery 0.000645 0.000484 0.000706 0.000448 0.000836 0.000655 0.000305 0.000432 0.000724 0.000586 0.000474 0.000440
Cytoplasm 0.576203* 0.549980* 0.405319* 0.500824* 0.572496* 0.515268* 0.513202* 0.558992* 0.574667* 0.529349* 0.467889* 0.531394*
Cytoplasmic Foci 0.144900 0.198149* 0.028982 0.041589 0.194672* 0.173111 0.060310 0.034530 0.165520 0.183268* 0.047152 0.037879
Eisosomes 0.001202 0.000356 0.000154 0.000177 0.001109 0.000799 0.000163 0.000199 0.001163 0.000619 0.000159 0.000189
Endoplasmic Reticulum 0.000756 0.001059 0.007410 0.001015 0.001591 0.001041 0.000684 0.000754 0.001102 0.001048 0.003509 0.000878
Endosome 0.018028 0.020587 0.004564 0.007647 0.018740 0.027416 0.006606 0.003609 0.018323 0.024646 0.005748 0.005525
Golgi 0.005663 0.006468 0.000235 0.006420 0.008545 0.008508 0.000777 0.003184 0.006857 0.007680 0.000549 0.004719
Lipid Particles 0.003807 0.003239 0.002732 0.000726 0.007002 0.003890 0.001546 0.000560 0.005131 0.003626 0.002044 0.000639
Mitochondria 0.001826 0.001256 0.000549 0.004516 0.002112 0.004120 0.000557 0.002988 0.001945 0.002958 0.000554 0.003713
Mitotic Spindle 0.000895 0.000524 0.011364 0.023923 0.000286 0.002517 0.008803 0.014348 0.000643 0.001708 0.009879 0.018891
None 0.001837 0.001368 0.003329 0.000810 0.001689 0.000867 0.001941 0.000262 0.001776 0.001070 0.002524 0.000522
Nuclear Periphery 0.000081 0.000218 0.001130 0.000240 0.000113 0.000090 0.000278 0.000118 0.000094 0.000142 0.000636 0.000176
Nuclear Periphery Foci 0.000359 0.000808 0.003847 0.001087 0.000694 0.000274 0.001080 0.000673 0.000498 0.000490 0.002242 0.000869
Nucleolus 0.000388 0.000307 0.000269 0.000119 0.000326 0.000305 0.000267 0.000103 0.000363 0.000306 0.000268 0.000111
Nucleus 0.044617 0.032463 0.244096* 0.022829 0.046546 0.031634 0.063597 0.023934 0.045416 0.031970 0.139411* 0.023410
Peroxisomes 0.003922 0.007561 0.002439 0.005461 0.003207 0.009620 0.002580 0.003535 0.003626 0.008785 0.002521 0.004449
Vacuole 0.109438 0.091844 0.176236 0.209916* 0.086283 0.115980 0.257052* 0.197794 0.099845 0.106189 0.223107* 0.203546*
Vacuole Periphery 0.001103 0.001164 0.000210 0.003849 0.001088 0.001504 0.000436 0.000725 0.001097 0.001366 0.000341 0.002207

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.53 -4.30 -4.74 -3.86 -2.01 -5.33 -1.48 -7.26 -5.20 -6.63 -3.98 -3.82 -8.33 -6.40 -6.34
Bud Neck 7.56 7.27 14.15 5.20 4.52 -5.12 3.07 6.45 11.17 1.91 1.51 8.08 15.21 12.33 4.20
Bud Site -6.34 -5.35 -13.12 -6.84 -4.85 -10.38 -5.56 -15.47 -7.67 -7.21 -11.28 -7.27 -19.89 -10.35 -8.69
Cell Periphery 2.01 -0.63 9.48 1.90 7.37 1.72 7.34 6.37 4.40 -3.07 2.20 6.99 10.41 4.67 4.25
Cytoplasm 2.75 21.46 10.94 6.61 -11.68 6.38 8.69 4.76 -1.50 -4.68 6.97 19.52 10.96 3.42 -10.30
Cytoplasmic Foci -8.22 31.69 28.74 28.18 -5.23 3.65 27.39 36.02 32.18 7.34 -4.11 38.04 45.17 42.58 3.71
Eisosomes 10.34 13.56 13.83 6.35 1.55 2.98 12.39 12.07 8.59 -1.59 7.74 18.30 18.21 10.09 -0.28
Endoplasmic Reticulum -1.84 -15.91 -0.77 1.02 15.60 1.87 3.40 3.56 1.44 0.48 0.30 -11.88 2.15 1.67 13.70
Endosome -1.34 13.42 11.65 9.96 -0.14 -4.29 9.26 13.59 14.87 3.81 -4.54 14.85 18.16 17.65 2.96
Golgi -0.87 17.95 2.62 2.74 -3.50 0.10 9.99 6.42 6.84 -1.63 -1.24 16.43 6.52 6.90 -3.58
Lipid Particles 1.34 1.70 11.40 6.15 13.79 4.96 9.78 11.98 9.13 3.48 4.16 9.70 16.37 10.92 9.66
Mitochondria 1.86 5.05 -0.74 -1.55 -2.42 -2.29 3.11 0.43 2.36 -1.82 -1.98 5.10 -0.19 1.36 -2.98
Mitotic Spindle 0.79 -3.59 -6.99 -7.32 -1.57 -3.16 -3.93 -5.26 -3.74 0.12 -2.16 -5.24 -8.61 -7.69 -1.07
None 0.96 -1.57 2.67 2.01 4.59 1.80 -0.41 5.01 1.59 2.86 1.99 -1.10 4.80 2.27 4.64
Nuclear Periphery -1.07 -22.44 -6.04 -0.69 17.76 1.33 -6.22 -1.79 -2.92 4.85 -0.92 -19.76 -6.00 -1.58 15.45
Nuclear Periphery Foci -4.61 -11.84 -7.43 -1.59 9.30 3.12 -1.98 0.02 -7.74 2.48 -0.10 -10.72 -4.83 -5.41 8.71
Nucleolus 2.03 3.07 11.33 5.10 5.93 0.16 0.52 6.82 2.74 2.71 1.04 1.70 12.09 4.14 4.24
Nucleus 4.59 -24.94 3.37 -1.66 25.70 6.30 -6.15 6.30 -0.52 10.65 7.70 -21.83 6.80 -1.63 24.00
Peroxisomes -3.53 4.28 0.60 3.54 -2.69 -6.85 0.95 0.67 6.37 -0.21 -7.37 3.30 1.07 7.21 -1.70
Vacuole 2.96 -14.26 -26.06 -26.53 -4.98 -6.45 -24.50 -27.96 -21.92 3.41 -2.44 -27.36 -37.53 -33.76 -0.61
Vacuole Periphery -0.34 11.44 -1.60 -1.51 -3.32 -3.11 5.01 4.11 5.94 -0.54 -2.74 8.70 -0.24 0.71 -3.22
ORF
Human Ortholog
Description Protein of unknown function; may interact with ribosomes, based on co-purification experiments
Localization
Cell Percentages cytoplasm (83%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

YDR239C

YDR239C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ydr239c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available