Standard name
Human Ortholog
Description Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.1 0.07 0.07 0.08 0.08 0.07 0.07 0.05 0 0.07 0.14 0.18 0.07 0.11 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.45 0 0.21 0.31 0.25 0.48 0.6 0.54 0.67 0.67 0.62 0.73 0 0.05 0 0.1 0 0 0 0 0 0 0 0
Nucleus 0.83 0.94 0.85 0.79 0.77 0.69 0.65 0.67 0.61 0.65 0.64 0.64 0.91 0.81 0.8 0.8 0.77 0.81 0.78 0.79 0.75 0.73 0.7 0.62
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0 0 0 0 0 0 0.06 0 0.09 0.14 0.11 0 0 0 0 0 0 0.06 0.09 0.08 0.11 0.12 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0.06 0.1 0.07 0.06 0.06 0.07 0.08 0.08 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 1 0 0 0 3 3 2 3 2 2 0 0 0 0 1 0 0 1 3 1 0 3
Bud Neck 0 0 0 0 1 0 0 1 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 3
Bud Site 0 0 0 0 0 7 5 16 3 9 3 11 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 2 7 12 9 21 25 32 17 20 12 10 10 26 37 12 18 14 0 0 0 0 2 1
Endoplasmic Reticulum 0 1 1 0 0 0 0 0 0 0 1 0 0 1 0 6 2 5 0 0 1 0 0 0
Endosome 0 0 2 0 2 2 0 0 0 0 0 0 0 0 1 6 7 1 1 2 2 1 2 3
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 3 1 0 0 1 0 0
Mitochondria 58 7 38 39 33 138 186 222 174 203 140 167 6 9 2 17 6 5 2 3 5 0 1 3
Nucleus 106 162 157 98 102 199 202 276 158 199 143 147 125 151 169 139 127 122 99 147 148 91 124 105
Nuclear Periphery 0 0 1 2 0 1 6 2 3 1 3 3 0 1 0 0 1 0 1 0 0 0 0 0
Nucleolus 10 2 6 3 4 8 13 23 8 27 31 26 4 6 3 6 1 4 7 16 16 13 21 15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 1 0 0
SpindlePole 0 4 0 0 1 2 5 8 0 1 2 0 1 2 1 4 1 3 2 1 3 1 6 8
Vac/Vac Membrane 3 7 2 1 6 15 10 8 5 5 2 6 2 3 5 11 16 11 8 11 13 9 14 19
Unique Cell Count 128 172 185 124 132 288 310 410 258 305 225 230 138 187 210 173 166 150 127 187 198 124 178 170
Labelled Cell Count 181 185 215 155 159 393 455 591 371 468 340 372 150 199 218 203 181 168 127 187 198 124 178 170


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.4 5.0 4.6 4.6 4.0 3.5 3.8 3.8 3.6 3.7 3.4 5.1 5.1 4.9 6.7 8.5 7.9 5.0 5.3 5.9
Std Deviation (1e-4) 0.6 1.0 1.6 1.6 1.7 1.2 1.1 1.1 1.5 1.3 1.1 1.0 1.0 1.8 1.2 2.1 3.5 2.4 1.4 1.6 1.2
Intensity Change (Log2) – – – -0.11 -0.11 -0.31 -0.53 -0.41 -0.39 -0.48 -0.44 -0.54 0.04 0.04 -0.02 0.43 0.76 0.66 -0.01 0.08 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.1 1.2 1.6 1.9 1.8 1.3 1.3 0.7 0.3 1.4 3.4 4.4 1.3 2.6 2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -4.2 -4.6 -6.4 -2.8 -4.8 -4.7
Nucleus -1.3 -1.7 -3.9 -4.8 -4.5 -5.4 -4.7 -4.8 -4.8 1.5 -1.1 -1.1 -1.1 -2.0 -0.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.4 0 0 0 0 0 0 0 0 0 2.7 3.6 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3124 1.422 1.4832 1.0223 0.9985 1.4193 2.3358 2.1547 2.2789 2.011 2.3032 2.6189 3.3745 3.0564 3.4817 3.4529 3.6291 3.5361
Actin 0.0025 0 0.0106 0.0009 0.0011 0.0019 0.0286 0 0.0182 0.0118 0.0058 0.0008 0.0147 0 0.0003 0.028 0.0018 0.0001
Bud 0.0003 0 0.0002 0 0.0011 0.0001 0.0008 0 0.0004 0.0005 0.0008 0.0002 0.0007 0 0.0001 0.0011 0.0001 0
Bud Neck 0.0028 0.0001 0.0004 0.0002 0.0009 0.0017 0.006 0.0002 0.0006 0.0002 0.0009 0.0023 0.0023 0.0001 0.0002 0.004 0.0018 0.0009
Bud Periphery 0.0011 0 0.0006 0 0.0017 0.0002 0.0036 0 0.0007 0.0012 0.0009 0.0005 0.0007 0 0.0002 0.0009 0.0003 0
Bud Site 0.0013 0 0.0006 0.0001 0.0081 0.0002 0.0035 0.0002 0.0029 0.0022 0.002 0.0003 0.0046 0 0.0002 0.036 0.0007 0
Cell Periphery 0.0004 0 0.0003 0 0.0002 0 0.0017 0 0.0002 0.0003 0.0001 0.0001 0.0002 0 0.0003 0.0005 0.0001 0
Cytoplasm 0.0084 0.0003 0.0014 0.0005 0.0027 0.0004 0.0031 0.0011 0.0006 0.0002 0.0013 0.0004 0.0031 0.0015 0.0018 0.0119 0.0017 0.0004
Cytoplasmic Foci 0.0051 0 0.002 0.0124 0.0054 0.0004 0.0084 0 0.0149 0.0005 0.0188 0.0006 0.0073 0 0.0005 0.0087 0.0025 0
Eisosomes 0.0001 0 0.0001 0 0.0001 0 0.0013 0 0.0001 0.0004 0 0 0.0002 0 0.0008 0.0011 0.0001 0
Endoplasmic Reticulum 0.0034 0.0003 0.002 0.0002 0.0007 0.0009 0.0039 0.0001 0.0004 0.0002 0.0003 0.0001 0.0016 0 0.0026 0.0027 0.0026 0
Endosome 0.0132 0.0001 0.0085 0.0068 0.0155 0.0037 0.0271 0.0001 0.0104 0.0015 0.0155 0.0024 0.0056 0 0.0032 0.0085 0.0148 0.0002
Golgi 0.002 0 0.0026 0.002 0.0038 0.0014 0.0087 0 0.0066 0.0019 0.013 0.0013 0.0021 0 0.0002 0.005 0.0019 0
Lipid Particles 0.0087 0 0.0025 0.0093 0.0212 0.0017 0.0247 0 0.0107 0.0013 0.0013 0.0003 0.007 0 0.0005 0.0132 0.0193 0
Mitochondria 0.008 0 0.0204 0.0003 0.0182 0.0019 0.0173 0.0001 0.0228 0.0592 0.0301 0.0025 0.0019 0.0001 0.0007 0.0076 0.0045 0.0001
None 0.0096 0 0.0011 0.0004 0.0004 0.0004 0.0077 0.0013 0.0014 0.0001 0.0005 0.0002 0.0039 0.0028 0.0085 0.0051 0.0018 0.0007
Nuclear Periphery 0.0109 0.0117 0.0087 0.0013 0.0074 0.0101 0.0164 0.0024 0.002 0.0011 0.0091 0.0009 0.0113 0.001 0.0023 0.0059 0.0334 0.0015
Nucleolus 0.0274 0.025 0.0109 0.0181 0.0486 0.0533 0.02 0.0293 0.0127 0.0198 0.0247 0.043 0.0185 0.0143 0.0193 0.0139 0.047 0.0357
Nucleus 0.8627 0.9595 0.9097 0.9266 0.8397 0.912 0.7647 0.9583 0.8783 0.8946 0.8277 0.9258 0.8716 0.9776 0.9227 0.8002 0.8312 0.945
Peroxisomes 0.0041 0 0.0083 0.0085 0.0108 0.0004 0.0074 0 0.0104 0.0008 0.026 0.0002 0.0105 0 0.0001 0.0081 0.0011 0
Punctate Nuclear 0.0219 0.0025 0.0071 0.0121 0.002 0.0084 0.0381 0.0068 0.0032 0.0012 0.0193 0.0174 0.0308 0.0025 0.032 0.0359 0.0281 0.0151
Vacuole 0.0047 0.0001 0.0011 0.0001 0.0051 0.0005 0.0046 0.0001 0.0018 0.0007 0.0011 0.0005 0.001 0 0.003 0.001 0.0027 0.0001
Vacuole Periphery 0.0013 0.0001 0.0008 0.0001 0.0052 0.0005 0.0026 0 0.0009 0.0005 0.0007 0.0002 0.0005 0 0.0006 0.0009 0.0027 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.3098 9.3369 6.4335 11.8636 8.2219 5.9651 10.1148 10.9229 7.4835 12.851
Translational Efficiency 0.7558 0.9741 1.2398 0.5735 1.0639 1.6214 0.8408 0.7642 0.9369 0.9886

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2016 1271 86 1584 2017 1630 210 716 4033 2901 296 2300

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 595.00 698.84 971.09 891.80 726.82 746.64 1002.84 881.19 660.93 725.70 993.62 888.50
Standard Deviation 69.68 100.61 98.26 123.46 83.91 99.37 121.83 137.48 101.45 102.69 116.37 128.08
Intensity Change Log 2 — 0.232073 0.706715 0.583831 — 0.038815 0.464421 0.277855 — 0.129023 0.578545 0.423659

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000054 0.000806 0.001476 0.000921 0.000068 0.000621 0.001544 0.001060 0.000061 0.000702 0.001524 0.000964
Bud Neck 0.002942 0.005971 0.003416 0.028027 0.001587 0.007973 0.009768 0.017862 0.002264 0.007096 0.007923 0.024862
Bud Site 0.001533 0.001435 0.000957 0.005590 0.000178 0.002440 0.001510 0.009829 0.000855 0.002000 0.001349 0.006909
Cell Periphery 0.000320 0.000179 0.000102 0.000111 0.000056 0.000168 0.000068 0.000245 0.000188 0.000173 0.000078 0.000153
Cytoplasm 0.104883* 0.036615 0.008270 0.006375 0.019140 0.021297 0.001629 0.037314 0.062001 0.028008 0.003559 0.016007
Cytoplasmic Foci 0.015507 0.005806 0.002418 0.002106 0.001818 0.007387 0.000059 0.003729 0.008661 0.006694 0.000744 0.002611
Eisosomes 0.000036 0.000037 0.000089 0.000026 0.000013 0.000042 0.000048 0.000040 0.000024 0.000040 0.000060 0.000031
Endoplasmic Reticulum 0.000918 0.005051 0.008508 0.006705 0.001053 0.004789 0.009088 0.006214 0.000986 0.004904 0.008919 0.006552
Endosome 0.001015 0.001367 0.000741 0.004265 0.000182 0.001976 0.000328 0.009759 0.000599 0.001709 0.000448 0.005975
Golgi 0.000405 0.000541 0.000177 0.002868 0.000062 0.002022 0.000125 0.001704 0.000234 0.001373 0.000141 0.002506
Lipid Particles 0.002183 0.002487 0.001495 0.000281 0.000527 0.005645 0.000308 0.002109 0.001355 0.004261 0.000653 0.000850
Mitochondria 0.002038 0.004990 0.000929 0.004180 0.000537 0.008961 0.001242 0.005556 0.001287 0.007221 0.001151 0.004609
Mitotic Spindle 0.000308 0.004804 0.000696 0.023209 0.000028 0.009529 0.001214 0.035592 0.000168 0.007458 0.001063 0.027063
None 0.046284 0.024831 0.002458 0.004137 0.009424 0.011270 0.002626 0.010723 0.027849 0.017212 0.002577 0.006187
Nuclear Periphery 0.001241 0.001592 0.000580 0.003484 0.000798 0.003932 0.000764 0.002239 0.001020 0.002907 0.000711 0.003097
Nuclear Periphery Foci 0.000690 0.001884 0.000740 0.001036 0.000236 0.001725 0.000300 0.004540 0.000463 0.001795 0.000428 0.002127
Nucleolus 0.018835 0.034458 0.018730 0.008050 0.013587 0.030722 0.012176 0.011109 0.016210 0.032359 0.014080 0.009002
Nucleus 0.798611* 0.862635* 0.938012* 0.886278* 0.950004* 0.872111* 0.952817* 0.818947* 0.874326* 0.867959* 0.948516* 0.865318*
Peroxisomes 0.000717 0.002230 0.000751 0.000256 0.000346 0.004655 0.000419 0.000694 0.000532 0.003593 0.000516 0.000392
Vacuole 0.001224 0.001639 0.009250 0.008502 0.000279 0.002086 0.003823 0.015948 0.000752 0.001890 0.005400 0.010820
Vacuole Periphery 0.000255 0.000643 0.000203 0.003593 0.000078 0.000649 0.000143 0.004786 0.000166 0.000646 0.000160 0.003965

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -13.21 -8.18 -24.02 -4.97 2.68 -10.24 -10.28 -10.82 -6.51 1.47 -16.19 -12.69 -22.47 -8.85 3.94
Bud Neck -6.29 -2.02 -19.79 -17.15 -16.96 -10.02 -11.32 -12.29 -7.56 -3.50 -11.53 -10.55 -23.35 -18.05 -12.52
Bud Site -0.22 0.41 -4.57 -4.89 -5.18 -5.04 -8.25 -4.88 -3.44 -3.29 -3.83 -5.15 -7.16 -5.39 -5.04
Cell Periphery 4.74 7.14 7.94 5.12 0.14 -6.24 -2.91 -4.79 -0.45 -3.62 0.72 6.32 3.46 2.97 -3.43
Cytoplasm 14.06 19.68 25.68 10.61 0.09 -0.98 9.14 -2.90 -2.28 -7.75 12.23 24.05 19.16 7.18 -6.61
Cytoplasmic Foci 5.57 8.95 11.54 5.49 1.11 -5.96 5.45 0.03 5.81 -4.41 1.63 11.64 10.81 8.31 -1.52
Eisosomes -1.03 -6.06 1.08 2.21 6.35 -12.11 -9.17 -15.58 -2.92 0.77 -9.27 -9.62 -10.97 0.68 6.11
Endoplasmic Reticulum -17.01 -8.89 -24.48 -9.34 0.77 -14.06 -11.64 -15.82 -7.35 1.46 -21.27 -14.40 -29.09 -11.93 2.02
Endosome -0.89 0.54 -2.25 -1.54 -2.97 -6.48 -5.43 -3.53 -2.13 -3.26 -4.34 -0.16 -4.55 -2.39 -4.66
Golgi -1.37 1.77 -1.75 -1.37 -2.20 -4.96 -4.55 -3.08 2.69 -2.38 -5.02 0.18 -2.60 0.80 -2.65
Lipid Particles -1.24 2.67 11.92 7.76 5.02 -5.84 1.13 -1.43 4.33 -1.63 -5.72 5.22 4.31 6.99 0.67
Mitochondria -4.20 3.26 -3.51 1.82 -7.23 -8.72 -10.31 -5.86 4.25 -4.27 -9.44 -0.46 -7.34 5.03 -7.74
Mitotic Spindle -3.77 -1.04 -7.38 -4.66 -7.05 -5.77 -2.14 -6.82 -4.56 -4.61 -6.88 -2.18 -10.05 -6.06 -5.84
None 6.45 16.45 16.18 10.57 -2.31 -1.12 5.32 0.93 1.93 -4.50 5.79 16.53 15.54 10.23 -3.58
Nuclear Periphery -1.65 3.94 -5.71 -4.88 -7.20 -4.63 -2.17 -6.09 1.72 -4.59 -4.80 0.12 -7.59 -1.15 -7.44
Nuclear Periphery Foci -3.63 -0.53 -0.86 2.78 -0.35 -4.72 -1.78 -2.06 -0.53 -1.70 -5.79 -1.19 -2.48 1.30 -1.85
Nucleolus -7.45 0.11 9.35 14.98 2.55 -9.75 -1.21 1.66 9.89 1.72 -11.83 -0.69 9.05 18.43 3.11
Nucleus -7.10 -12.58 -12.05 -4.20 4.59 13.20 3.03 12.41 3.55 8.16 2.08 -8.15 0.79 -1.11 8.13
Peroxisomes -3.90 -0.15 6.16 5.14 4.68 -5.43 -0.16 -0.30 5.48 -0.27 -6.55 0.36 2.86 7.30 3.83
Vacuole -1.68 -2.23 -8.62 -8.17 -0.34 -7.19 -5.58 -8.47 -7.77 -5.57 -6.08 -5.07 -12.18 -11.06 -3.99
Vacuole Periphery -2.74 0.30 -2.93 -2.10 -2.98 -5.84 -5.95 -3.14 -2.28 -2.98 -5.75 -1.19 -4.24 -3.00 -4.15
Standard name
Human Ortholog
Description Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
Localization
Cell Percentages nucleus (96%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Snu56

Snu56


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snu56-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available