Standard name | |
---|---|
Human Ortholog | |
Description | Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); human homolog is TRAPPC4 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 81 | 24 | 26 | 8 | 8 | 14 | 5 | 2 | 3 | 5 | 1 | 10 | 24 | 23 | 14 | 45 | 45 | 28 | 49 | 16 | 30 | 19 | 14 | 24 |
Bud | 14 | 2 | 19 | 7 | 17 | 21 | 16 | 43 | 28 | 29 | 29 | 37 | 3 | 3 | 0 | 9 | 5 | 3 | 4 | 0 | 1 | 1 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 7 | 5 | 2 | 6 | 9 | 3 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 1 | 1 | 1 | 0 | 0 | 2 | 1 | 1 | 4 | 2 | 2 | 0 | 1 | 2 | 4 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 56 | 15 | 19 | 32 | 30 | 53 | 39 | 29 | 40 | 18 | 20 | 36 | 17 | 20 | 26 | 29 | 22 | 22 | 13 | 4 | 2 | 3 | 2 | 7 |
Endoplasmic Reticulum | 20 | 17 | 6 | 4 | 9 | 16 | 13 | 10 | 6 | 11 | 6 | 5 | 16 | 26 | 12 | 7 | 5 | 6 | 4 | 0 | 2 | 4 | 4 | 10 |
Endosome | 127 | 10 | 113 | 58 | 75 | 26 | 58 | 37 | 56 | 27 | 11 | 39 | 60 | 59 | 79 | 43 | 51 | 43 | 206 | 46 | 111 | 56 | 52 | 60 |
Golgi | 145 | 33 | 22 | 22 | 12 | 4 | 3 | 2 | 3 | 8 | 1 | 3 | 15 | 33 | 36 | 70 | 56 | 43 | 215 | 51 | 91 | 62 | 53 | 88 |
Mitochondria | 87 | 17 | 90 | 65 | 122 | 264 | 323 | 419 | 345 | 359 | 253 | 368 | 3 | 7 | 4 | 27 | 29 | 15 | 15 | 6 | 19 | 22 | 21 | 16 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 1 | 1 | 2 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 2 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 |
Nucleolus | 3 | 0 | 3 | 2 | 1 | 10 | 17 | 35 | 15 | 16 | 9 | 10 | 0 | 1 | 1 | 1 | 5 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 5 | 0 | 31 | 8 | 9 | 0 | 19 | 9 | 22 | 9 | 5 | 23 | 2 | 2 | 3 | 1 | 1 | 2 | 30 | 4 | 19 | 8 | 5 | 5 |
SpindlePole | 14 | 2 | 25 | 15 | 10 | 12 | 15 | 21 | 29 | 37 | 15 | 21 | 4 | 13 | 24 | 13 | 18 | 13 | 11 | 1 | 7 | 1 | 3 | 1 |
Vac/Vac Membrane | 22 | 1 | 7 | 4 | 6 | 5 | 12 | 33 | 18 | 17 | 6 | 4 | 2 | 3 | 14 | 12 | 10 | 15 | 11 | 3 | 2 | 10 | 3 | 4 |
Unique Cell Count | 429 | 83 | 245 | 177 | 240 | 364 | 433 | 547 | 453 | 435 | 303 | 454 | 126 | 164 | 187 | 191 | 172 | 151 | 571 | 141 | 291 | 197 | 169 | 225 |
Labelled Cell Count | 579 | 122 | 362 | 226 | 299 | 434 | 529 | 645 | 573 | 553 | 362 | 568 | 146 | 193 | 216 | 263 | 253 | 195 | 571 | 141 | 291 | 197 | 169 | 225 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.1 | 9.1 | 6.8 | 7.0 | 6.4 | 6.0 | 5.5 | 5.4 | 5.4 | 4.8 | 5.1 | 5.4 | 8.7 | 8.5 | 8.4 | 9.8 | 9.7 | 10.1 | 9.6 | 10.0 | 9.8 |
Std Deviation (1e-4) | 1.4 | 1.1 | 1.2 | 1.7 | 1.6 | 1.6 | 1.4 | 1.2 | 1.2 | 1.1 | 1.1 | 1.3 | 1.1 | 1.1 | 1.2 | 1.8 | 1.9 | 2.0 | 1.4 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.09 | -0.19 | -0.31 | -0.35 | -0.34 | -0.52 | -0.43 | -0.35 | 0.35 | 0.31 | 0.3 | 0.52 | 0.51 | 0.56 | 0.49 | 0.55 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.3 | -3.1 | -3.3 | -5.7 | -7.2 | -6.3 | 0 | -5.5 | -4.8 | 2.3 | 1.0 | -1.1 | 3.6 | 4.2 | 2.2 |
Bud | -1.6 | -0.3 | -1.0 | -2.3 | 0.1 | -0.8 | 0 | 0.7 | 0.2 | -2.1 | -2.6 | -3.9 | -1.3 | -2.1 | -2.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.2 | 1.7 | 2.6 | 0.6 | -1.3 | 0.5 | -2.0 | -0.5 | 0.1 | 1.8 | 1.5 | 2.1 | 2.5 | 1.7 | 2.2 |
Endoplasmic Reticulum | 0 | 0.8 | 1.3 | 0.4 | 0 | 0 | 0 | -0.4 | 0 | 4.0 | 4.9 | 2.0 | 0.7 | 0 | 0 |
Endosome | -2.8 | -3.4 | -11.2 | -9.4 | -13.1 | -9.9 | 0 | -11.8 | -11.5 | 0.3 | -2.0 | -0.8 | -5.1 | -3.4 | -3.5 |
Golgi | 1.1 | -1.7 | -4.7 | -5.5 | -6.5 | -5.6 | 0 | -5.0 | -5.7 | 0.9 | 3.2 | 3.1 | 7.0 | 6.1 | 5.1 |
Mitochondria | 0 | 3.1 | 8.8 | 9.7 | 10.8 | 10.3 | 0 | 11.2 | 11.8 | -7.2 | -7.6 | -8.6 | -5.3 | -4.4 | -5.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1.3 | 2.0 | 3.1 | 1.7 | 0 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -2.8 | -3.6 | -7.0 | -4.0 | -6.5 | -3.7 | 0 | -5.2 | -3.6 | -3.5 | -4.2 | -4.2 | -4.8 | -4.6 | -4.0 |
SpindlePole | -0.6 | -2.6 | -3.5 | -3.6 | -3.5 | -1.8 | 0 | -2.4 | -2.8 | -2.4 | -0.8 | 0.9 | -1.2 | 0.1 | -0.5 |
Vacuole | 0 | -0.2 | 0 | -0.1 | 1.9 | 0.8 | 0.7 | -0.7 | 0 | 0 | 0 | 2.2 | 1.7 | 1.5 | 3.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.5666 | 3.899 | 3.5769 | 2.7405 | 3.1448 | 3.358 | 4.5404 | 6.0762 | 5.1233 | 5.0288 | 4.9559 | 5.6589 | 3.3916 | 4.177 | 3.5178 | 3.6552 | 3.3458 | 3.7442 |
Actin | 0.0403 | 0.0105 | 0.0378 | 0.0028 | 0.0029 | 0.0136 | 0.0698 | 0.0921 | 0.0413 | 0.0795 | 0.0709 | 0.0391 | 0.03 | 0.0113 | 0.019 | 0.009 | 0.0049 | 0.0198 |
Bud | 0.0009 | 0.0012 | 0.0004 | 0.0006 | 0.0001 | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0004 | 0.0004 | 0.0005 | 0.0004 | 0.0002 | 0.0002 |
Bud Neck | 0.0057 | 0.0007 | 0.0009 | 0.0004 | 0.0003 | 0.0038 | 0.0006 | 0.0007 | 0.0004 | 0.0005 | 0.0008 | 0.001 | 0.0007 | 0.0007 | 0.0009 | 0.0227 | 0.0003 | 0.0016 |
Bud Periphery | 0.0012 | 0.0005 | 0.0003 | 0.0005 | 0.0001 | 0.0003 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0006 | 0.0001 | 0.0002 |
Bud Site | 0.0046 | 0.0094 | 0.002 | 0.0007 | 0.0002 | 0.0007 | 0.0014 | 0.0028 | 0.0006 | 0.0007 | 0.0035 | 0.0003 | 0.0015 | 0.0038 | 0.0076 | 0.004 | 0.0004 | 0.0006 |
Cell Periphery | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0009 | 0.0001 | 0 |
Cytoplasm | 0.0309 | 0.0716 | 0.0199 | 0.0298 | 0.0037 | 0.0077 | 0.0226 | 0.0193 | 0.0054 | 0.007 | 0.0103 | 0.0073 | 0.031 | 0.0259 | 0.0112 | 0.0097 | 0.0047 | 0.0089 |
Cytoplasmic Foci | 0.248 | 0.3197 | 0.2702 | 0.2283 | 0.2556 | 0.2509 | 0.2963 | 0.2756 | 0.2486 | 0.2434 | 0.3164 | 0.2384 | 0.2476 | 0.3151 | 0.2814 | 0.2534 | 0.2409 | 0.2505 |
Eisosomes | 0.0003 | 0 | 0.0013 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0066 | 0.0034 | 0.0015 | 0.0012 | 0.0028 | 0.0014 | 0.0024 | 0.001 | 0.0005 | 0.0005 | 0.0027 | 0.0006 | 0.0037 | 0.0005 | 0.0017 | 0.0069 | 0.0013 | 0.0005 |
Endosome | 0.332 | 0.2904 | 0.2806 | 0.4025 | 0.3534 | 0.2565 | 0.2282 | 0.2004 | 0.2298 | 0.2278 | 0.2241 | 0.215 | 0.3193 | 0.3105 | 0.2684 | 0.3169 | 0.3459 | 0.2348 |
Golgi | 0.1735 | 0.2087 | 0.2463 | 0.2242 | 0.2081 | 0.3331 | 0.2495 | 0.261 | 0.331 | 0.3168 | 0.2466 | 0.3096 | 0.1983 | 0.2318 | 0.2712 | 0.2727 | 0.2458 | 0.3185 |
Lipid Particles | 0.0294 | 0.0051 | 0.0186 | 0.013 | 0.0408 | 0.0133 | 0.0248 | 0.0052 | 0.0157 | 0.0037 | 0.0083 | 0.0163 | 0.0353 | 0.0083 | 0.0119 | 0.0174 | 0.0238 | 0.0188 |
Mitochondria | 0.0121 | 0.0046 | 0.0097 | 0.0089 | 0.004 | 0.0286 | 0.0047 | 0.0041 | 0.0044 | 0.065 | 0.0023 | 0.0046 | 0.0092 | 0.004 | 0.0116 | 0.0125 | 0.0042 | 0.004 |
None | 0.0092 | 0.0015 | 0.0114 | 0.0006 | 0.0008 | 0.0004 | 0.0026 | 0.0123 | 0.0002 | 0.0003 | 0.0024 | 0.0005 | 0.0064 | 0.0002 | 0.0033 | 0.003 | 0.0006 | 0.0081 |
Nuclear Periphery | 0.0121 | 0.0009 | 0.0016 | 0.0003 | 0.02 | 0.005 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0149 | 0.0001 | 0.0099 | 0.0001 | 0.0008 | 0.0107 | 0.0145 | 0.0001 |
Nucleolus | 0.0006 | 0.0001 | 0.001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0007 | 0 | 0.0001 | 0.0004 | 0.0012 | 0 |
Nucleus | 0.0046 | 0.0004 | 0.001 | 0.0003 | 0.0007 | 0.0007 | 0.0001 | 0.0001 | 0 | 0 | 0.0035 | 0 | 0.0024 | 0.0001 | 0.0004 | 0.0007 | 0.0093 | 0.0001 |
Peroxisomes | 0.0785 | 0.0671 | 0.0838 | 0.0788 | 0.1015 | 0.0795 | 0.0934 | 0.1221 | 0.1196 | 0.0522 | 0.0765 | 0.1649 | 0.0959 | 0.0837 | 0.1068 | 0.0474 | 0.0813 | 0.1313 |
Punctate Nuclear | 0.0035 | 0.0014 | 0.0089 | 0.0003 | 0.0019 | 0.0011 | 0.0008 | 0.0004 | 0.0003 | 0.0002 | 0.0144 | 0.0003 | 0.0026 | 0.0004 | 0.0006 | 0.0015 | 0.0023 | 0.0004 |
Vacuole | 0.0036 | 0.0018 | 0.0017 | 0.0044 | 0.0011 | 0.0013 | 0.0007 | 0.0012 | 0.0009 | 0.0005 | 0.0008 | 0.0007 | 0.0028 | 0.0021 | 0.0011 | 0.006 | 0.0114 | 0.0009 |
Vacuole Periphery | 0.0018 | 0.0008 | 0.0011 | 0.0022 | 0.0018 | 0.0015 | 0.0005 | 0.0006 | 0.0007 | 0.0008 | 0.0007 | 0.0007 | 0.0017 | 0.0009 | 0.0008 | 0.0027 | 0.0069 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.6873 | 7.4725 | 11.0899 | 17.2729 | 8.6704 | 8.3545 | 11.711 | 14.2784 | 13.8417 | 12.7991 |
Translational Efficiency | 2.7465 | 1.848 | 1.6129 | 0.9021 | 1.9057 | 2.198 | 1.711 | 2.0539 | 1.6634 | 1.8264 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
418 | 1031 | 2239 | 1755 | 2363 | 1751 | 1901 | 1477 | 2781 | 2782 | 4140 | 3232 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 782.60 | 915.00 | 1077.19 | 1205.74 | 867.81 | 879.68 | 1095.51 | 1179.69 | 855.00 | 892.77 | 1085.60 | 1193.84 |
Standard Deviation | 104.29 | 127.77 | 152.02 | 168.19 | 124.86 | 119.73 | 134.02 | 163.79 | 125.73 | 123.95 | 144.32 | 166.70 |
Intensity Change Log 2 | — | 0.225497 | 0.460926 | 0.623572 | — | 0.019600 | 0.336152 | 0.442957 | — | 0.120902 | 0.396665 | 0.531425 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003372 | 0.005451 | 0.003955 | 0.033981 | 0.001706 | 0.003394 | 0.025500 | 0.028216 | 0.001956 | 0.004156 | 0.013848 | 0.031346 |
Bud Neck | 0.002728 | 0.000571 | 0.001811 | 0.002095 | 0.000922 | 0.000790 | 0.000617 | 0.001642 | 0.001194 | 0.000709 | 0.001263 | 0.001888 |
Bud Site | 0.009883 | 0.005734 | 0.005875 | 0.057962 | 0.002309 | 0.004988 | 0.014698 | 0.044209 | 0.003448 | 0.005264 | 0.009927 | 0.051677 |
Cell Periphery | 0.000174 | 0.000057 | 0.000035 | 0.000140 | 0.000024 | 0.000028 | 0.000041 | 0.000092 | 0.000047 | 0.000039 | 0.000038 | 0.000118 |
Cytoplasm | 0.012515 | 0.007167 | 0.007465 | 0.095484 | 0.007219 | 0.005800 | 0.011114 | 0.054111 | 0.008015 | 0.006306 | 0.009140 | 0.076577 |
Cytoplasmic Foci | 0.270020* | 0.345220* | 0.350300* | 0.315632* | 0.269707* | 0.318380* | 0.423073* | 0.345249* | 0.269754* | 0.328327* | 0.383716* | 0.329167* |
Eisosomes | 0.000155 | 0.000046 | 0.000028 | 0.000061 | 0.000053 | 0.000054 | 0.000046 | 0.000058 | 0.000068 | 0.000051 | 0.000036 | 0.000060 |
Endoplasmic Reticulum | 0.000563 | 0.000223 | 0.000342 | 0.000307 | 0.000082 | 0.000137 | 0.000259 | 0.000405 | 0.000154 | 0.000168 | 0.000304 | 0.000352 |
Endosome | 0.178759 | 0.151614 | 0.224675* | 0.137425 | 0.136554 | 0.163186 | 0.178463 | 0.165860 | 0.142897 | 0.158898 | 0.203455* | 0.150420 |
Golgi | 0.427795* | 0.420205* | 0.285751* | 0.196236* | 0.532919* | 0.446838* | 0.229526* | 0.235515* | 0.517118* | 0.436968* | 0.259934* | 0.214186* |
Lipid Particles | 0.015338 | 0.005568 | 0.019761 | 0.006255 | 0.008128 | 0.005525 | 0.004883 | 0.003382 | 0.009212 | 0.005541 | 0.012929 | 0.004942 |
Mitochondria | 0.027206 | 0.008724 | 0.003904 | 0.002974 | 0.014547 | 0.012056 | 0.001359 | 0.002600 | 0.016449 | 0.010821 | 0.002735 | 0.002803 |
Mitotic Spindle | 0.007189 | 0.015718 | 0.059572 | 0.059956 | 0.005767 | 0.010082 | 0.054857 | 0.044236 | 0.005980 | 0.012170 | 0.057407 | 0.052772 |
None | 0.000057 | 0.000064 | 0.000720 | 0.000025 | 0.000143 | 0.000064 | 0.000009 | 0.000013 | 0.000130 | 0.000064 | 0.000393 | 0.000020 |
Nuclear Periphery | 0.000069 | 0.000022 | 0.000168 | 0.000048 | 0.000012 | 0.000012 | 0.000043 | 0.000038 | 0.000020 | 0.000016 | 0.000110 | 0.000044 |
Nuclear Periphery Foci | 0.000282 | 0.000636 | 0.003171 | 0.000559 | 0.000604 | 0.000095 | 0.000463 | 0.000585 | 0.000556 | 0.000296 | 0.001927 | 0.000571 |
Nucleolus | 0.000190 | 0.000109 | 0.000826 | 0.000064 | 0.000484 | 0.000047 | 0.000008 | 0.000021 | 0.000440 | 0.000070 | 0.000450 | 0.000044 |
Nucleus | 0.000528 | 0.000152 | 0.000498 | 0.000289 | 0.000173 | 0.000043 | 0.000048 | 0.000107 | 0.000227 | 0.000084 | 0.000292 | 0.000206 |
Peroxisomes | 0.028715 | 0.027330 | 0.010444 | 0.060083 | 0.013803 | 0.024247 | 0.044379 | 0.048616 | 0.016044 | 0.025389 | 0.026026 | 0.054843 |
Vacuole | 0.008769 | 0.003265 | 0.017315 | 0.027658 | 0.002777 | 0.002567 | 0.009461 | 0.022934 | 0.003678 | 0.002825 | 0.013709 | 0.025499 |
Vacuole Periphery | 0.005694 | 0.002125 | 0.003383 | 0.002766 | 0.002068 | 0.001668 | 0.001155 | 0.002109 | 0.002613 | 0.001837 | 0.002360 | 0.002466 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.26 | -0.46 | -10.57 | -9.62 | -11.05 | -2.26 | -10.88 | -9.78 | -8.93 | -0.68 | -3.14 | -10.84 | -15.29 | -13.55 | -7.85 |
Bud Neck | 4.61 | 1.50 | 0.80 | -11.10 | -1.37 | 0.60 | 1.79 | -4.90 | -4.88 | -8.98 | 2.58 | -0.63 | -5.67 | -9.54 | -4.28 |
Bud Site | 1.79 | 1.85 | -15.79 | -20.80 | -21.32 | -3.57 | -10.27 | -17.85 | -16.18 | -11.36 | -2.93 | -9.03 | -28.14 | -26.43 | -23.34 |
Cell Periphery | 3.38 | 4.16 | 0.84 | -4.53 | -6.43 | -0.85 | -4.32 | -8.97 | -8.90 | -7.01 | 1.11 | 1.28 | -6.75 | -8.10 | -8.54 |
Cytoplasm | 1.66 | 1.60 | -18.20 | -21.20 | -21.60 | 1.17 | -3.57 | -14.73 | -15.27 | -13.19 | 1.40 | -1.76 | -25.21 | -26.13 | -25.05 |
Cytoplasmic Foci | -8.09 | -9.36 | -4.12 | 5.49 | 7.17 | -9.42 | -27.63 | -11.54 | -3.16 | 12.28 | -13.26 | -26.42 | -11.38 | 1.56 | 13.50 |
Eisosomes | 5.33 | 6.27 | 4.74 | -2.38 | -7.71 | -0.20 | 1.48 | -0.80 | -0.46 | -2.52 | 2.82 | 6.49 | 1.88 | -1.50 | -7.26 |
Endoplasmic Reticulum | 2.42 | 1.55 | 1.80 | -1.65 | 0.58 | -1.34 | -4.94 | -2.19 | -1.77 | -0.85 | -0.41 | -4.56 | -2.80 | -2.49 | -0.57 |
Endosome | 2.89 | -4.80 | 5.34 | 3.62 | 16.47 | -5.62 | -9.01 | -4.86 | 0.36 | 3.08 | -3.95 | -14.56 | -0.51 | 3.62 | 14.71 |
Golgi | 0.55 | 10.97 | 18.65 | 27.22 | 13.93 | 11.40 | 46.21* | 39.13* | 27.00 | 0.26 | 12.41 | 44.47 | 51.54* | 40.00 | 10.64 |
Lipid Particles | 2.87 | -1.34 | 2.33 | -1.66 | 6.65 | 1.86 | 2.78 | 3.94 | 1.80 | 2.55 | 3.00 | -2.81 | 3.11 | -0.31 | 6.99 |
Mitochondria | 5.06 | 6.81 | 7.20 | 4.08 | 1.53 | 1.54 | 11.46 | 8.63 | 6.73 | -1.34 | 4.00 | 12.03 | 11.80 | 8.22 | 0.13 |
Mitotic Spindle | -2.50 | -13.37 | -13.07 | -10.82 | -0.86 | -3.24 | -14.67 | -11.48 | -10.03 | 1.83 | -4.61 | -21.16 | -18.57 | -15.51 | 0.50 |
None | -0.46 | -1.61 | 1.69 | 1.49 | 1.67 | 0.83 | 1.99 | 1.90 | 1.26 | -4.43 | 0.89 | -1.15 | 1.86 | 1.56 | 1.65 |
Nuclear Periphery | 1.25 | -2.15 | 0.17 | -5.65 | 3.39 | -0.03 | -4.26 | -7.54 | -7.45 | -0.38 | 0.73 | -5.01 | -5.63 | -10.43 | 3.20 |
Nuclear Periphery Foci | -1.51 | -5.53 | -3.80 | -0.25 | 4.89 | 2.58 | 0.48 | -0.36 | -3.52 | -1.03 | 1.38 | -4.56 | -0.75 | -3.25 | 4.49 |
Nucleolus | 0.83 | -2.67 | 1.20 | 0.64 | 3.10 | 2.08 | 2.28 | 2.20 | 0.94 | -6.06 | 2.06 | -0.53 | 2.16 | 0.61 | 3.05 |
Nucleus | 1.05 | -0.09 | 0.12 | -2.56 | 0.46 | 2.19 | 1.96 | -0.34 | -3.64 | -3.35 | 1.83 | -1.03 | -1.38 | -4.08 | -0.27 |
Peroxisomes | 0.32 | 5.34 | -6.70 | -8.12 | -16.52 | -5.07 | -11.24 | -11.77 | -7.26 | -0.96 | -5.30 | -6.29 | -17.01 | -11.41 | -11.68 |
Vacuole | 2.80 | -4.06 | -10.27 | -17.56 | -8.08 | 0.58 | -9.16 | -12.66 | -12.78 | -8.66 | 1.86 | -12.82 | -20.63 | -21.66 | -11.80 |
Vacuole Periphery | 3.51 | 2.31 | 2.91 | -1.12 | 1.47 | 1.80 | 4.02 | -0.02 | -1.77 | -3.92 | 3.05 | 0.97 | 0.70 | -2.13 | -0.16 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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