ORF
Human Ortholog
Description Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.07 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.14 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.97 0.98 0.98 0.88 0.89 – 0.75 0.78 0.55 0.69 0.99 0.99 0.99 1.0 0.96 1.0 0.86 0.95 0.87 0.86 0.85 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.1 0 0 0 0.25 0 – 0.23 0.26 0.51 0.38 0 0 0 0 0 0 0.05 0 0.07 0.05 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.07 – 0.1 0.06 0.09 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 1 5 3
Bud 0 0 0 0 0 3 1 – 1 7 5 5 0 0 1 0 0 0 0 0 0 2 16 16
Bud Neck 0 0 2 2 9 1 8 – 4 2 2 0 0 0 0 0 0 0 0 0 0 2 5 3
Bud Site 0 0 0 0 0 2 0 – 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 4 2 1 1 3 – 2 3 0 0 2 1 0 2 6 0 0 0 0 1 4 1
Cytoplasm 154 59 28 247 348 200 403 – 231 277 93 105 418 655 685 215 179 67 153 76 56 264 541 465
Endoplasmic Reticulum 1 1 0 0 1 0 1 – 0 1 0 0 1 0 0 7 6 3 0 0 0 1 1 2
Endosome 0 0 0 0 0 0 5 – 1 0 1 0 0 1 1 0 1 0 1 0 0 1 2 2
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 2 0 0 1 0 0 0 0 1 1 1
Mitochondria 12 6 0 4 0 58 19 – 72 94 85 58 0 0 0 0 3 2 8 1 4 14 27 12
Nucleus 1 0 0 1 2 9 5 – 7 8 4 3 1 0 0 1 0 0 0 0 0 1 0 0
Nuclear Periphery 0 1 0 0 1 0 0 – 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 – 1 0 1 0 0 0 1 0 0 0 0 0 0 0 1 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 5 1 4 – 3 5 1 5 0 1 0 0 0 0 2 0 0 3 13 8
Vac/Vac Membrane 1 0 0 2 2 2 31 – 32 20 15 8 6 1 8 4 8 1 5 2 0 4 4 2
Unique Cell Count 162 60 29 252 355 228 451 307 355 168 152 424 660 690 216 187 67 178 80 65 306 635 527
Labelled Cell Count 170 67 34 260 369 278 480 355 419 207 184 428 662 697 229 204 73 178 80 65 306 635 527


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.0 5.6 4.8 5.5 3.7 4.8 – 4.8 4.6 4.6 4.5 5.0 5.2 5.6 7.4 8.2 6.5 4.5 4.6 4.9
Std Deviation (1e-4) 1.0 0.9 1.1 1.2 1.5 1.2 1.3 – 2.0 1.5 1.9 1.6 1.2 1.8 2.0 2.7 2.9 2.1 1.5 1.7 1.7
Intensity Change (Log2) – – – -0.23 -0.02 -0.59 -0.22 – -0.2 -0.27 -0.27 -0.33 -0.17 -0.11 0.01 0.41 0.56 0.22 -0.33 -0.28 -0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2327 -0.1938 -0.1039 -0.4211 0.6502 -0.1742 0.6656 1.6092 1.2041 1.0254 1.0835 1.4625 3.3827 2.9933 2.3752 2.8605 2.4818 2.6132
Actin 0.0328 0.0001 0.0411 0.0384 0.0136 0.0063 0.0075 0.0002 0.0014 0.0003 0.0009 0.0027 0.059 0.0001 0.0082 0.0294 0.0084 0.0009
Bud 0.0007 0.0001 0.0002 0.0015 0.0012 0.0008 0 0.0003 0.0002 0.0001 0.0015 0.0002 0.0016 0.0001 0.0005 0.0007 0.0002 0.0001
Bud Neck 0.0017 0.0001 0.0005 0.0016 0.0057 0.0007 0.0002 0.0002 0.0002 0.0003 0.0002 0.005 0.003 0 0.0017 0.0006 0.0001 0.0003
Bud Periphery 0.0008 0.0001 0.0003 0.0032 0.0018 0.0018 0.0001 0.0001 0.0001 0.0001 0.0023 0.0003 0.0021 0 0.0003 0.0006 0.0006 0
Bud Site 0.0044 0.0006 0.0011 0.0084 0.0163 0.0004 0.0001 0.0007 0.0002 0.0001 0.0017 0.0001 0.0098 0.0002 0.0067 0.0041 0.0002 0
Cell Periphery 0.0003 0.0001 0.0001 0.0003 0.0004 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0002 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.222 0.2936 0.3325 0.2831 0.3813 0.4228 0.5497 0.6199 0.5236 0.6804 0.5692 0.6887 0.2665 0.3883 0.4158 0.4939 0.4287 0.5749
Cytoplasmic Foci 0.031 0.0107 0.0105 0.0161 0.0295 0.0184 0.0076 0.0058 0.0066 0.0039 0.0205 0.014 0.0127 0.0027 0.0162 0.0122 0.0152 0.0036
Eisosomes 0.0004 0.0001 0.0003 0.0007 0.0003 0.0001 0.0003 0.0001 0.0001 0 0.0001 0 0.0003 0.0001 0.0001 0.0002 0.0001 0
Endoplasmic Reticulum 0.0053 0.002 0.0027 0.0033 0.01 0.0021 0.0042 0.0028 0.0021 0.002 0.0011 0.0028 0.0034 0.001 0.003 0.0034 0.0025 0.001
Endosome 0.0396 0.0016 0.0056 0.0237 0.0564 0.0108 0.004 0.0024 0.0018 0.0014 0.008 0.0063 0.0134 0.0005 0.0113 0.01 0.0101 0.0008
Golgi 0.0079 0.0001 0.0034 0.0058 0.0136 0.0005 0.0008 0.0002 0.0002 0.0001 0.0033 0.0005 0.0064 0.0001 0.0013 0.0047 0.0044 0.0001
Lipid Particles 0.0269 0.0002 0.0067 0.0219 0.0145 0.0015 0.0007 0.0003 0.0002 0 0.0007 0.0007 0.0052 0.0001 0.0013 0.0024 0.0223 0.0001
Mitochondria 0.0144 0.0001 0.0019 0.0045 0.0092 0.0006 0.0004 0.0003 0.0003 0.0002 0.003 0.0006 0.0019 0.0001 0.0009 0.0013 0.0007 0.0001
None 0.5129 0.687 0.5714 0.567 0.3385 0.5197 0.4076 0.3593 0.4582 0.3037 0.3672 0.2668 0.5764 0.6044 0.5157 0.4221 0.4548 0.4155
Nuclear Periphery 0.03 0.0004 0.005 0.0047 0.0458 0.0025 0.0065 0.0009 0.0008 0.001 0.0004 0.002 0.0032 0.0003 0.0078 0.0024 0.0037 0.0003
Nucleolus 0.0034 0.0001 0.0002 0.001 0.0083 0.0003 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0057 0 0.0002 0.0003 0.0003 0
Nucleus 0.0306 0.001 0.0074 0.0023 0.0209 0.0041 0.0069 0.0024 0.0019 0.0046 0.0028 0.0055 0.0021 0.0009 0.0022 0.0018 0.0008 0.0016
Peroxisomes 0.0146 0.0007 0.0052 0.004 0.008 0.0008 0.0001 0.0001 0.0002 0.0001 0.013 0.0003 0.0035 0 0.0027 0.0039 0.0264 0.0001
Punctate Nuclear 0.013 0.0008 0.0025 0.0054 0.0142 0.0045 0.0011 0.0027 0.0008 0.0006 0.0024 0.0024 0.0218 0.0003 0.002 0.005 0.0199 0.0003
Vacuole 0.0052 0.0005 0.0009 0.0018 0.0059 0.0009 0.0012 0.001 0.0007 0.0008 0.001 0.0009 0.001 0.0005 0.0014 0.0006 0.0003 0.0002
Vacuole Periphery 0.0021 0.0001 0.0003 0.0011 0.0046 0.0002 0.0004 0.0001 0.0001 0.0001 0.0002 0.0002 0.0005 0 0.0008 0.0002 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.9068 8.474 13.8338 17.2883 12.2905 17.0535 19.2782 15.6138 18.4135 18.0422
Translational Efficiency 0.7565 1.6941 0.9365 0.9262 1.3087 1.219 1.2145 1.3263 1.1742 1.1899

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1945 983 1847 347 1388 1418 2879 967 3333 2401 4726 1314

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 675.99 824.06 887.51 783.98 668.10 716.65 819.74 833.57 672.70 760.63 846.23 820.47
Standard Deviation 78.80 110.01 138.35 107.82 93.71 90.98 106.16 112.66 85.42 112.39 124.25 113.52
Intensity Change Log 2 — 0.285747 0.392761 0.213815 — 0.101205 0.295102 0.319239 — 0.196966 0.345045 0.267181

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000182 0.000499 0.000168 0.000349 0.000062 0.001525 0.000067 0.001851 0.000132 0.001105 0.000106 0.001455
Bud Neck 0.025702 0.018148 0.004534 0.004515 0.006159 0.037623 0.004279 0.005689 0.017564 0.029649 0.004378 0.005379
Bud Site 0.005319 0.012100 0.002659 0.014653 0.003561 0.020416 0.005012 0.035543 0.004587 0.017012 0.004092 0.030027
Cell Periphery 0.000153 0.000144 0.000091 0.000136 0.000103 0.000163 0.000081 0.000161 0.000132 0.000155 0.000085 0.000154
Cytoplasm 0.432046* 0.482520* 0.400503* 0.387648* 0.570285* 0.435417* 0.580739* 0.551324* 0.489614* 0.454701* 0.510300* 0.508100*
Cytoplasmic Foci 0.242749* 0.191242* 0.004005 0.052434 0.146408 0.209801* 0.010017 0.018258 0.202629* 0.202202* 0.007667 0.027283
Eisosomes 0.000205 0.000080 0.000015 0.000058 0.000074 0.000155 0.000016 0.000040 0.000151 0.000125 0.000015 0.000045
Endoplasmic Reticulum 0.000853 0.001989 0.001812 0.002316 0.000551 0.000717 0.000831 0.001666 0.000727 0.001238 0.001215 0.001838
Endosome 0.012224 0.009823 0.000817 0.010598 0.005203 0.014497 0.000637 0.003826 0.009300 0.012583 0.000708 0.005614
Golgi 0.005456 0.003988 0.000062 0.000933 0.001269 0.004088 0.000039 0.002129 0.003712 0.004047 0.000048 0.001813
Lipid Particles 0.010493 0.003332 0.000452 0.001675 0.003914 0.005659 0.000418 0.000895 0.007753 0.004706 0.000431 0.001101
Mitochondria 0.013090 0.004309 0.000625 0.004787 0.002502 0.009150 0.000506 0.002275 0.008681 0.007168 0.000552 0.002939
Mitotic Spindle 0.003988 0.008198 0.012605 0.011059 0.001213 0.005728 0.012716 0.030201 0.002832 0.006739 0.012672 0.025146
None 0.002344 0.002975 0.008036 0.012373 0.005463 0.002492 0.005635 0.011364 0.003643 0.002690 0.006574 0.011630
Nuclear Periphery 0.000521 0.000692 0.001592 0.001193 0.000581 0.000417 0.001392 0.001143 0.000546 0.000529 0.001470 0.001156
Nuclear Periphery Foci 0.000229 0.000823 0.000522 0.000990 0.000352 0.000303 0.000279 0.001006 0.000280 0.000516 0.000374 0.001002
Nucleolus 0.001064 0.000966 0.000324 0.003044 0.000820 0.001684 0.000209 0.000401 0.000963 0.001390 0.000254 0.001099
Nucleus 0.194026* 0.198323* 0.538142* 0.379869* 0.221128* 0.151380* 0.345145* 0.235057* 0.205312* 0.170599* 0.420571* 0.273299*
Peroxisomes 0.010178 0.006545 0.000062 0.002264 0.002183 0.022235 0.000071 0.000976 0.006849 0.015811 0.000068 0.001316
Vacuole 0.037926 0.051895 0.022864 0.107289 0.027545 0.074747 0.031850 0.095067 0.033603 0.065391 0.028338 0.098294
Vacuole Periphery 0.001252 0.001410 0.000110 0.001818 0.000621 0.001806 0.000062 0.001129 0.000989 0.001644 0.000081 0.001311

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.33 -2.59 -1.33 1.26 -0.45 -2.37 -3.30 -1.78 1.03 -1.70 -2.64 -1.59 -1.75 0.98 -1.65
Bud Neck 3.43 12.08 12.51 7.61 1.17 -14.05 0.82 0.24 13.79 -0.35 -6.87 12.17 11.29 15.40 0.22
Bud Site -5.07 3.19 -1.44 0.67 -2.12 -8.80 -0.68 -4.41 1.81 -4.29 -10.25 1.03 -4.48 1.47 -4.89
Cell Periphery 0.75 6.19 3.90 2.61 -0.46 -5.50 4.47 -0.98 3.82 -4.07 -2.56 8.57 2.60 4.40 -3.52
Cytoplasm -4.25 9.96 6.71 8.93 1.23 13.03 7.87 14.08 1.77 8.89 4.99 7.88 11.27 6.55 6.00
Cytoplasmic Foci 6.27 45.55 31.66 19.42 -8.03 -8.80 29.81 28.29 34.74 -4.40 0.23 52.72 46.75 40.28 -9.48
Eisosomes 11.45 19.33 13.48 1.59 -12.17 -5.32 17.15 5.41 6.73 -15.41 2.47 22.45 15.34 7.12 -19.30
Endoplasmic Reticulum -5.63 -12.46 -3.86 -1.44 -0.01 -1.92 -8.14 -14.54 -13.86 -9.37 -5.14 -13.26 -11.54 -8.01 -4.77
Endosome 2.18 13.57 2.58 1.53 -2.29 -7.79 8.79 4.77 11.01 -4.38 -3.50 16.00 7.25 9.64 -3.81
Golgi 1.44 9.79 8.39 3.44 -1.79 -4.62 3.63 1.33 5.83 -1.89 -0.34 10.38 7.18 6.15 -2.24
Lipid Particles 9.34 14.13 12.47 4.85 -7.42 -3.11 10.08 8.00 10.46 -11.94 5.95 16.58 14.75 11.76 -13.55
Mitochondria 5.37 8.67 5.97 1.30 -1.54 -5.26 2.36 1.65 7.89 -1.39 1.52 8.92 7.45 7.37 -2.03
Mitotic Spindle -1.79 -5.75 -1.81 -0.68 1.42 -3.62 -8.87 -5.42 -3.52 0.05 -3.02 -9.59 -5.11 -3.21 0.75
None -1.01 -14.78 -2.28 -2.05 -0.33 3.16 -0.51 -1.60 -5.27 -1.65 2.18 -6.57 -3.91 -5.09 -1.19
Nuclear Periphery -3.20 -22.39 -3.57 -3.03 -0.31 1.05 -10.38 -9.79 -17.15 -1.20 -0.40 -21.19 -10.61 -11.04 -0.93
Nuclear Periphery Foci -4.29 -8.36 -4.81 -1.26 -1.88 0.84 0.04 -9.80 -10.02 -10.12 -3.76 -6.96 -11.52 -7.37 -8.74
Nucleolus 1.06 4.45 -0.63 -0.79 -1.21 -2.81 7.29 1.98 3.55 -2.08 -1.83 6.95 0.29 1.24 -1.66
Nucleus -0.80 -44.69 -10.85 -10.04 10.36 8.83 -23.31 -8.72 -16.28 10.09 5.92 -46.18 -14.77 -18.27 16.50
Peroxisomes 3.30 12.98 9.06 4.90 -1.71 -10.43 4.75 3.93 11.72 -2.61 -6.93 13.69 11.85 12.85 -2.73
Vacuole -5.46 -3.05 -12.88 -11.29 -12.07 -13.40 -9.80 -22.54 -15.11 -19.23 -13.71 -8.93 -25.65 -19.44 -22.87
Vacuole Periphery -0.46 8.48 0.66 0.84 -1.49 -2.97 3.50 0.95 3.54 -2.23 -2.44 8.83 2.19 3.66 -2.53
ORF
Human Ortholog
Description Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1
Localization
Cell Percentages cytoplasm (22%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YDR248C

YDR248C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ydr248c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available