ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.98 0.98 0.98 0.88 0.82 0.85 0.79 0.78 0.76 0.71 1.0 0.99 0.99 0.96 0.95 0.94 0.94 0.95 0.95 0.98 0.95 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.2 0.06 0.3 0.42 0.3 0.48 0.49 0.47 0.54 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 0 1 2
Bud 0 2 5 1 2 3 16 6 10 18 11 8 1 1 0 1 0 0 3 2 0 0 3 3
Bud Neck 0 0 1 0 0 1 1 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 1 1 0 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 1 3 1 0 0 3 2 4 0 4 0 0 2 38 25 24 0 0 0 0 0 0
Cytoplasm 623 420 462 444 513 529 681 634 469 472 332 385 508 364 548 467 453 503 611 412 453 296 439 377
Endoplasmic Reticulum 3 4 1 0 0 1 0 0 0 0 2 2 0 0 0 19 14 19 0 2 1 0 0 2
Endosome 0 0 0 0 0 1 0 3 0 0 1 0 0 0 0 1 1 1 2 1 2 0 0 3
Golgi 1 0 0 0 1 0 0 0 0 0 0 0 0 1 0 3 3 4 3 0 0 0 0 0
Mitochondria 12 0 24 91 29 178 345 226 284 299 205 292 1 1 0 4 12 8 12 4 3 1 0 3
Nucleus 2 3 1 0 2 2 7 6 3 1 4 4 1 0 0 0 0 6 0 0 0 0 2 0
Nuclear Periphery 0 0 0 1 0 0 5 1 0 0 0 5 0 0 0 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 3 1 0 1 2 0 0 0 0 0 0 6 1 1 0 1 0
Vac/Vac Membrane 3 1 0 1 4 6 5 5 9 9 3 8 0 2 3 17 23 39 0 2 1 2 1 2
Unique Cell Count 640 427 470 454 524 599 828 748 596 606 437 543 510 366 551 484 475 534 652 436 475 304 462 403
Labelled Cell Count 646 430 495 541 552 723 1062 887 779 804 561 716 511 369 553 550 531 605 652 436 475 304 462 403


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 6.7 3.9 3.8 4.5 3.5 3.3 3.6 3.0 3.1 3.2 3.0 6.7 6.7 6.8 7.9 7.9 7.6 6.6 6.7 6.9
Std Deviation (1e-4) 0.8 0.9 0.8 1.1 1.1 0.9 1.0 1.0 1.0 0.7 1.1 0.7 1.0 1.1 1.1 1.5 1.4 1.6 1.0 1.1 1.2
Intensity Change (Log2) – – – -0.04 0.19 -0.19 -0.26 -0.12 -0.41 -0.35 -0.32 -0.41 0.77 0.76 0.78 1.01 1.0 0.95 0.75 0.77 0.82


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 6.0 4.7 4.3
Cytoplasm -0.6 -0.5 -6.2 -8.6 -7.7 -9.6 -9.8 -10.2 -11.7 2.0 1.5 1.8 -1.7 -2.6 -3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 4.0 3.4 3.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -4.9 -4.1 -5.4 -3.9 -2.1 -3.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 4.1 4.8 6.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4172 3.0449 2.7789 2.1745 3.0334 2.6626 1.2641 1.4213 1.5789 1.8203 0.9058 1.5733 3.1346 3.9738 3.6544 3.2077 3.7024 3.3846
Actin 0.0274 0.0022 0.0092 0.0108 0.0483 0.0073 0.0254 0.0011 0.0126 0.1 0.0082 0.0095 0.0155 0.0007 0.0124 0.0001 0.0044 0.0016
Bud 0.0009 0.0031 0.0012 0.0096 0.0052 0.003 0.0032 0.005 0.0013 0.0009 0.0007 0.0008 0.0015 0.0003 0.0032 0.0002 0.0006 0.0021
Bud Neck 0.0019 0.0006 0.0006 0.0021 0.0024 0.003 0.0081 0.0005 0.0008 0.0046 0.0094 0.0038 0.0023 0.0005 0.0058 0.0004 0.0009 0.0032
Bud Periphery 0.0011 0.001 0.0007 0.0051 0.0109 0.0017 0.0085 0.0047 0.0026 0.001 0.0018 0.0011 0.0021 0.0002 0.0029 0.0001 0.001 0.0023
Bud Site 0.0027 0.0115 0.0022 0.0033 0.0041 0.0005 0.0091 0.0106 0.0061 0.0112 0.0108 0.0015 0.0145 0.0032 0.0059 0.0001 0.0014 0.0003
Cell Periphery 0.0002 0.0002 0.0001 0.0002 0.0002 0.0001 0.0006 0.0002 0.0006 0.0002 0.0003 0.0002 0.0002 0.0001 0.0003 0 0.0001 0
Cytoplasm 0.6205 0.807 0.7178 0.6758 0.5832 0.7622 0.5175 0.7285 0.6225 0.6724 0.6551 0.6332 0.5652 0.6952 0.586 0.7468 0.6247 0.6404
Cytoplasmic Foci 0.0148 0.0126 0.0129 0.0236 0.0444 0.0223 0.0153 0.0083 0.0178 0.0499 0.0229 0.0186 0.0154 0.0046 0.0101 0.0086 0.0112 0.0102
Eisosomes 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001 0.0003 0 0.0002 0.0005 0.0002 0.0001 0.0001 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0039 0.0023 0.0031 0.0016 0.0073 0.0024 0.0102 0.003 0.0061 0.0058 0.0079 0.0034 0.004 0.0017 0.0028 0.0011 0.0017 0.0019
Endosome 0.0118 0.0032 0.0055 0.0042 0.0602 0.0139 0.0227 0.0027 0.0147 0.0242 0.0222 0.0167 0.0091 0.0012 0.0054 0.0026 0.0034 0.003
Golgi 0.002 0.0005 0.0013 0.0034 0.0127 0.0041 0.004 0.0002 0.0058 0.007 0.0104 0.0046 0.0015 0.0001 0.001 0.0001 0.0007 0.0003
Lipid Particles 0.0012 0.0003 0.0018 0.0171 0.0074 0.0023 0.0043 0.0001 0.0034 0.0089 0.0021 0.0026 0.0007 0.0001 0.0015 0 0.0075 0.0001
Mitochondria 0.0074 0.0004 0.0018 0.0051 0.0069 0.0032 0.0071 0.0003 0.0078 0.0021 0.0078 0.0103 0.0014 0.0002 0.0008 0.0003 0.0016 0.0006
None 0.2611 0.1383 0.2237 0.2008 0.1195 0.1447 0.3093 0.2207 0.2687 0.0782 0.2106 0.2582 0.3397 0.2777 0.3354 0.2046 0.3051 0.3191
Nuclear Periphery 0.0124 0.0014 0.0023 0.0015 0.0179 0.0109 0.025 0.0018 0.0037 0.0056 0.0019 0.0075 0.0073 0.0016 0.0038 0.0018 0.0032 0.0012
Nucleolus 0.0008 0.0003 0.0003 0.0022 0.0018 0.0005 0.0041 0.0003 0.0013 0.0004 0.002 0.0011 0.0004 0.0002 0.0008 0.0005 0.0011 0.0001
Nucleus 0.013 0.0064 0.004 0.0131 0.0091 0.006 0.0088 0.0067 0.0117 0.0052 0.0097 0.0068 0.0078 0.0081 0.0121 0.0163 0.017 0.0083
Peroxisomes 0.005 0.0008 0.0022 0.0099 0.0306 0.0019 0.005 0.0002 0.0041 0.0081 0.0062 0.0106 0.0055 0.0001 0.0014 0.0001 0.007 0.0004
Punctate Nuclear 0.0083 0.0063 0.0079 0.0088 0.0194 0.0077 0.006 0.0036 0.0054 0.0105 0.0072 0.0068 0.0045 0.0036 0.0069 0.0153 0.0062 0.0039
Vacuole 0.0023 0.0013 0.001 0.0011 0.006 0.0013 0.0042 0.0014 0.0024 0.0026 0.002 0.0016 0.0012 0.0007 0.0013 0.0009 0.0009 0.0007
Vacuole Periphery 0.0012 0.0002 0.0003 0.0004 0.0024 0.0008 0.0014 0.0002 0.0005 0.0006 0.0005 0.0009 0.0003 0.0001 0.0003 0.0002 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
177 1599 221 1245 1442 1663 1853 248 1619 3262 2074 1493

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 645.63 815.14 965.72 956.92 554.23 781.47 844.03 1035.06 564.22 797.97 857.00 969.90
Standard Deviation 74.04 115.48 108.08 124.96 69.67 111.22 114.25 146.34 75.74 114.57 119.65 132.00
Intensity Change Log 2 — 0.336340 0.580897 0.567691 — 0.495706 0.606809 0.901158 — 0.412146 0.592924 0.731337

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000144 0.000366 0.000600 0.001016 0.000115 0.000224 0.000237 0.001342 0.000118 0.000294 0.000276 0.001070
Bud Neck 0.018068 0.016701 0.002184 0.007597 0.019677 0.016813 0.007464 0.003594 0.019501 0.016758 0.006901 0.006932
Bud Site 0.007923 0.014497 0.005639 0.053187 0.007153 0.012212 0.034142 0.031418 0.007237 0.013332 0.031105 0.049571
Cell Periphery 0.000321 0.000080 0.000291 0.000179 0.000190 0.000088 0.000074 0.000274 0.000204 0.000084 0.000097 0.000195
Cytoplasm 0.504510* 0.600954* 0.340003* 0.550872* 0.461087* 0.526779* 0.702038* 0.294576* 0.465834* 0.563139* 0.663460* 0.508299*
Cytoplasmic Foci 0.304839* 0.160221 0.026390 0.014977 0.218813* 0.169953 0.013328 0.052027 0.228218* 0.165182 0.014720 0.021131
Eisosomes 0.000232 0.000052 0.000112 0.000033 0.000157 0.000076 0.000019 0.000073 0.000165 0.000064 0.000029 0.000039
Endoplasmic Reticulum 0.000485 0.000899 0.006148 0.002049 0.001276 0.000468 0.000433 0.003815 0.001189 0.000679 0.001042 0.002342
Endosome 0.007687 0.013224 0.008866 0.004056 0.010795 0.022239 0.002821 0.039328 0.010455 0.017820 0.003465 0.009915
Golgi 0.003218 0.002737 0.000204 0.006787 0.002182 0.003832 0.000654 0.028766 0.002296 0.003295 0.000606 0.010438
Lipid Particles 0.005155 0.002347 0.004955 0.000960 0.013354 0.003440 0.000452 0.005554 0.012458 0.002904 0.000932 0.001723
Mitochondria 0.004375 0.002774 0.000367 0.001457 0.011322 0.006724 0.001383 0.010872 0.010562 0.004788 0.001275 0.003021
Mitotic Spindle 0.000240 0.008205 0.007616 0.048577 0.000531 0.009021 0.025263 0.097280 0.000499 0.008621 0.023382 0.056667
None 0.002481 0.003605 0.002773 0.003331 0.010598 0.002588 0.007523 0.005997 0.009711 0.003087 0.007017 0.003774
Nuclear Periphery 0.000181 0.000278 0.001712 0.000996 0.000809 0.000474 0.000573 0.001021 0.000740 0.000378 0.000694 0.001000
Nuclear Periphery Foci 0.000257 0.000686 0.005736 0.002323 0.000440 0.000337 0.000375 0.002742 0.000420 0.000508 0.000946 0.002393
Nucleolus 0.000676 0.000839 0.000704 0.000368 0.002318 0.000880 0.000428 0.000164 0.002138 0.000860 0.000457 0.000334
Nucleus 0.107102 0.081983 0.418050* 0.124435 0.184643* 0.082825 0.109534 0.063163 0.176165* 0.082412 0.142409* 0.114258
Peroxisomes 0.006861 0.005327 0.001617 0.000538 0.004338 0.006864 0.001065 0.003199 0.004614 0.006111 0.001124 0.000980
Vacuole 0.024324 0.083261 0.165739 0.171978 0.048629 0.132227 0.091099 0.312585* 0.045972 0.108225 0.099052 0.195334
Vacuole Periphery 0.000922 0.000961 0.000295 0.004284 0.001573 0.001938 0.001096 0.042210 0.001502 0.001459 0.001010 0.010584

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.92 -6.69 -4.86 -1.09 2.34 -2.90 -2.21 -4.16 -2.94 -2.65 -4.30 -3.30 -6.52 -2.74 -2.47
Bud Neck 0.42 4.74 4.26 7.72 -3.01 1.65 8.03 10.08 10.47 3.50 1.72 8.89 10.38 12.14 2.22
Bud Site -2.62 0.96 -3.51 -1.64 -5.19 -3.34 -5.11 -1.69 -0.49 0.89 -4.88 -4.94 -4.91 -2.16 -0.42
Cell Periphery 5.55 0.99 5.04 -1.99 6.92 4.02 5.12 2.70 -1.29 -2.91 5.20 5.22 4.28 -2.07 -2.12
Cytoplasm -4.53 8.67 4.34 16.48 -6.97 -5.96 -12.06 9.59 12.87 16.15 -10.87 -8.94 6.93 18.07 15.62
Cytoplasmic Foci 9.09 18.10 19.25 31.62 5.67 7.80 40.70 35.51 29.47 -2.39 11.27 44.07 44.50 46.61 1.77
Eisosomes 7.15 4.13 7.81 7.04 8.29 8.38 16.26 11.73 3.00 -6.87 12.36 16.56 16.41 7.41 -1.25
Endoplasmic Reticulum -1.51 -10.04 -9.15 -2.88 8.27 5.20 2.73 -2.55 -5.83 -4.43 2.73 -2.36 -3.47 -6.62 -1.41
Endosome -3.41 -0.57 4.50 12.21 8.17 -7.04 9.63 2.05 6.35 -2.01 -6.24 9.33 9.09 17.17 0.38
Golgi 0.61 3.18 1.83 1.95 -1.40 -2.08 6.84 -0.62 0.19 -1.73 -1.80 7.54 1.25 2.28 -1.89
Lipid Particles 4.48 0.60 7.05 3.50 5.88 9.24 12.26 10.25 2.60 -3.75 10.10 12.24 11.95 6.35 -2.67
Mitochondria 0.92 2.25 1.99 2.76 -4.48 2.57 6.58 5.79 3.76 -0.61 3.86 6.82 6.78 5.37 -0.21
Mitotic Spindle -5.14 -1.97 -9.21 -6.11 -4.89 -5.14 -10.59 -3.20 -1.83 0.38 -7.15 -10.75 -9.67 -6.44 -1.95
None -0.84 0.07 -2.36 -1.85 -5.86 5.68 3.03 3.34 -2.79 0.97 5.37 3.01 3.89 -3.67 1.66
Nuclear Periphery -3.37 -15.57 -22.90 -22.07 -0.52 2.33 -4.74 -5.10 -7.78 -1.64 3.30 -6.68 -11.67 -19.94 -7.34
Nuclear Periphery Foci -5.21 -7.99 -7.66 -5.17 5.70 1.73 -0.93 -8.27 -8.67 -8.15 -1.74 -6.79 -8.67 -8.09 -4.38
Nucleolus -1.31 0.48 2.64 5.10 2.98 4.16 5.60 6.34 6.98 4.25 4.19 5.61 6.14 7.80 2.98
Nucleus 2.38 -13.97 -6.29 -15.88 11.70 16.66 1.02 6.45 -3.59 6.03 17.54 -3.80 1.84 -16.63 5.89
Peroxisomes 1.15 4.11 5.21 7.94 2.12 -3.22 10.95 10.44 8.82 -0.33 -2.43 11.64 14.50 12.43 1.79
Vacuole -15.32 -12.89 -34.42 -26.33 -5.97 -19.12 -21.61 -22.46 -17.65 -16.05 -20.32 -24.49 -37.95 -28.67 -19.29
Vacuole Periphery 0.01 3.21 0.28 0.30 -1.09 -1.07 5.52 -1.04 -0.95 -1.39 0.63 5.87 0.10 -0.07 -1.44
ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
Localization
Cell Percentages cytoplasm (49%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YDR250C

YDR250C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ydr250c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available