Standard name
Human Ortholog
Description Heterotrimeric nascent polypeptide-associated complex beta3 subunit; complex binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3; BTT1 has a paralog, EGD1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.93 0.95 0.96 0.8 – 0.72 0.71 0.7 0.65 0.65 0.98 0.98 0.97 0.93 0.9 0.88 0.92 0.91 0.85 0.85 0.75 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.22 0.12 0.43 – 0.47 0.58 0.57 0.59 0.57 0 0 0 0 0.05 0 0 0 0.09 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.08 0.09 0.09 0 0 0 0 0.07 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1
Bud 1 3 2 3 1 9 – 20 7 9 11 14 0 1 1 0 0 0 2 0 0 0 2 10
Bud Neck 0 0 1 0 3 0 – 2 0 1 0 1 0 0 1 0 0 0 1 1 0 0 0 1
Bud Site 0 0 0 0 0 0 – 0 2 2 1 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 1 0 0 0 – 0 1 1 2 4 1 1 1 11 10 8 0 0 0 0 0 0
Cytoplasm 355 143 102 132 261 356 – 554 285 310 227 285 99 131 240 154 215 144 347 136 91 46 122 167
Endoplasmic Reticulum 1 1 1 1 0 0 – 1 0 0 0 0 1 1 2 9 3 8 0 0 0 0 3 2
Endosome 0 0 0 0 1 0 – 4 0 0 0 1 0 0 2 0 1 3 3 1 0 0 2 6
Golgi 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 3 3 0 1 1 0 1 5 3
Mitochondria 13 4 9 31 34 190 – 362 233 253 204 250 1 4 1 2 11 6 6 0 10 0 7 4
Nucleus 0 1 0 1 0 3 – 11 2 4 6 2 1 0 0 0 2 2 0 0 0 0 2 1
Nuclear Periphery 0 0 0 0 1 0 – 10 2 3 4 1 0 2 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 1 0 – 11 5 3 1 5 0 0 0 0 1 1 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
SpindlePole 0 0 0 0 0 1 – 3 1 2 1 4 0 0 1 0 0 0 0 0 0 0 0 2
Vac/Vac Membrane 1 1 1 0 1 0 – 10 11 8 7 5 0 0 1 13 22 14 6 6 2 2 10 6
Unique Cell Count 363 146 110 139 272 443 766 400 444 348 437 101 133 248 166 238 163 377 149 108 55 164 216
Labelled Cell Count 374 154 117 168 303 559 989 549 596 464 577 103 140 250 192 268 186 377 149 108 55 164 216


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.7 3.2 3.8 4.1 3.2 – 3.9 3.4 3.3 3.4 3.3 5.0 5.3 5.3 8.4 8.2 8.0 5.0 5.9 5.7
Std Deviation (1e-4) 0.6 1.0 0.5 1.3 1.8 1.0 – 2.6 2.7 1.9 3.1 2.6 1.0 1.4 1.8 1.8 1.8 1.4 0.8 2.3 1.9
Intensity Change (Log2) – – – 0.23 0.36 -0.02 – 0.27 0.08 0.04 0.08 0.04 0.64 0.72 0.72 1.38 1.35 1.31 0.63 0.87 0.84


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 1.3 -3.1 – -4.6 -4.7 -4.9 -5.6 -5.7 1.8 2.3 1.7 0 -0.7 -1.2
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 -1.8 0 0 -1.3 -1.6
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 2.6 2.9 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1202 0.6823 0.4742 0.5641 0.2164 0.7186 0.153 0.3663 0.3521 -0.0649 0.3115 0.2748 1.5068 2.3513 2.3116 2.1958 1.8244 2.2371
Actin 0.0165 0.0011 0.0084 0.0115 0.0251 0.0044 0.0245 0.0023 0.0165 0.0496 0.0406 0.0022 0.0094 0.0002 0.0052 0.0002 0.0005 0.0001
Bud 0.0004 0.0001 0.0005 0.0014 0.0003 0.0003 0.0036 0.0002 0.0021 0.0091 0.0008 0.0015 0.0001 0.0002 0.0006 0.0001 0.0001 0
Bud Neck 0.0019 0.0001 0.0017 0.0357 0.0012 0.0006 0.0039 0.0001 0.0006 0.0011 0.0005 0.0098 0.0001 0.0001 0.0056 0.0001 0.0001 0.0004
Bud Periphery 0.0005 0.0001 0.0006 0.0014 0.0004 0.0004 0.003 0.0002 0.0024 0.0073 0.0012 0.0011 0 0.0001 0.0006 0.0001 0.0001 0
Bud Site 0.0008 0.0013 0.0029 0.0054 0.0015 0.0003 0.0118 0.0015 0.0162 0.0135 0.0016 0.0001 0.0003 0.0012 0.0007 0.0001 0.0001 0
Cell Periphery 0.0002 0.0001 0.0002 0.0005 0.0001 0.0001 0.0008 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0
Cytoplasm 0.373 0.4595 0.488 0.4823 0.4641 0.6178 0.4864 0.6338 0.5717 0.5477 0.4952 0.6208 0.3985 0.6262 0.5979 0.4646 0.4781 0.6832
Cytoplasmic Foci 0.0373 0.0179 0.0215 0.044 0.0377 0.0211 0.0367 0.0106 0.0369 0.025 0.0356 0.0265 0.0095 0.0082 0.008 0.0066 0.009 0.0129
Eisosomes 0.0003 0.0001 0.0003 0.0001 0.0002 0.0001 0.0002 0 0.0002 0.0002 0.0001 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0098 0.0074 0.0035 0.0027 0.0042 0.0048 0.0085 0.003 0.0029 0.0028 0.0046 0.0045 0.0055 0.0034 0.0025 0.0018 0.0043 0.0028
Endosome 0.0268 0.0068 0.0133 0.0231 0.0468 0.0183 0.0324 0.0059 0.0232 0.0351 0.0749 0.0133 0.0041 0.0035 0.0085 0.003 0.0049 0.0097
Golgi 0.0081 0.0006 0.0018 0.0102 0.0101 0.0018 0.015 0.0003 0.0111 0.0146 0.0387 0.0021 0.0005 0.0002 0.001 0.0001 0.0003 0.0002
Lipid Particles 0.0082 0.0014 0.0034 0.0043 0.0315 0.0017 0.014 0.0002 0.0113 0.0069 0.0214 0.0127 0.0012 0.0001 0.0011 0.0001 0.0002 0.0001
Mitochondria 0.0041 0.0003 0.0018 0.0048 0.0204 0.0032 0.0112 0.0002 0.015 0.0283 0.0115 0.0008 0.0002 0.0002 0.0004 0.0003 0.0003 0.0003
None 0.4924 0.4978 0.4341 0.346 0.3205 0.3138 0.3031 0.3363 0.2485 0.2248 0.1562 0.2979 0.5642 0.3523 0.3617 0.5197 0.4981 0.2872
Nuclear Periphery 0.0044 0.0014 0.002 0.0022 0.0054 0.0026 0.004 0.0007 0.0026 0.002 0.0066 0.0018 0.0009 0.0007 0.001 0.0008 0.0008 0.0004
Nucleolus 0.0003 0.0001 0.0003 0.0009 0.0014 0.0005 0.0007 0.0001 0.0004 0.0004 0.0002 0.0001 0.0001 0.0001 0.0001 0 0 0
Nucleus 0.0022 0.001 0.0017 0.0049 0.0032 0.0024 0.0028 0.0012 0.0019 0.0026 0.0024 0.0014 0.0008 0.0013 0.0013 0.0011 0.0011 0.001
Peroxisomes 0.0045 0.0002 0.0094 0.0063 0.015 0.0012 0.025 0.0002 0.0269 0.0151 0.0984 0.0009 0.0024 0.0001 0.0006 0.0001 0.0002 0.0001
Punctate Nuclear 0.0044 0.0009 0.0018 0.0081 0.0068 0.0025 0.0069 0.0006 0.006 0.0018 0.005 0.0012 0.0007 0.0006 0.0016 0.0004 0.0005 0.0003
Vacuole 0.003 0.0015 0.0024 0.0033 0.0031 0.0014 0.004 0.0022 0.0027 0.0083 0.0027 0.0011 0.0013 0.001 0.0012 0.0007 0.0011 0.0011
Vacuole Periphery 0.0008 0.0002 0.0005 0.0007 0.001 0.0006 0.0015 0.0002 0.0007 0.0037 0.0017 0.0003 0.0001 0.0001 0.0003 0.0002 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1351 1198 132 1126 1590 33 1914 1648 2941 1231 2046 2774

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 679.08 745.95 1103.82 936.94 626.34 769.14 933.30 917.91 650.57 746.57 944.30 925.63
Standard Deviation 80.78 94.88 124.86 128.35 124.45 97.31 104.04 126.87 109.82 95.02 113.52 127.81
Intensity Change Log 2 — 0.135497 0.700851 0.464375 — 0.296300 0.575395 0.551407 — 0.214889 0.642017 0.506788

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000051 0.000264 0.001026 0.002716 0.000093 0.000214 0.000239 0.000468 0.000073 0.000262 0.000290 0.001381
Bud Neck 0.006327 0.020306 0.001860 0.005510 0.013661 0.085001 0.004179 0.007949 0.010292 0.022040 0.004029 0.006959
Bud Site 0.002372 0.015177 0.008143 0.045532 0.003432 0.015050 0.008064 0.037159 0.002945 0.015173 0.008069 0.040558
Cell Periphery 0.000143 0.000105 0.000432 0.000195 0.000132 0.000333 0.000099 0.000094 0.000137 0.000111 0.000120 0.000135
Cytoplasm 0.590955* 0.487731* 0.512743* 0.557749* 0.486996* 0.287544* 0.609451* 0.642042* 0.534751* 0.482364* 0.603212* 0.607826*
Cytoplasmic Foci 0.226475* 0.272245* 0.069237 0.038264 0.236371* 0.358689* 0.024069 0.031063 0.231825* 0.274562* 0.026983 0.033986
Eisosomes 0.000136 0.000101 0.000094 0.000040 0.000161 0.000386 0.000029 0.000029 0.000149 0.000109 0.000033 0.000033
Endoplasmic Reticulum 0.000844 0.000735 0.008633 0.002303 0.001042 0.000257 0.001234 0.000960 0.000951 0.000722 0.001711 0.001505
Endosome 0.008473 0.024923 0.016964 0.010482 0.014340 0.068807 0.002853 0.004678 0.011645 0.026100 0.003764 0.007034
Golgi 0.002281 0.006211 0.000799 0.008766 0.002790 0.006150 0.000755 0.003415 0.002556 0.006209 0.000758 0.005587
Lipid Particles 0.008560 0.006396 0.005949 0.001599 0.014196 0.010150 0.001156 0.000665 0.011607 0.006496 0.001465 0.001044
Mitochondria 0.003095 0.006269 0.000222 0.007239 0.006693 0.007291 0.001129 0.001955 0.005040 0.006297 0.001070 0.004100
Mitotic Spindle 0.001470 0.004107 0.009657 0.052351 0.000449 0.000138 0.008253 0.020712 0.000918 0.004001 0.008343 0.033554
None 0.004400 0.002822 0.001146 0.003617 0.006514 0.001060 0.003279 0.002638 0.005543 0.002775 0.003141 0.003035
Nuclear Periphery 0.000294 0.000293 0.000985 0.000843 0.000999 0.000172 0.000977 0.000443 0.000675 0.000290 0.000978 0.000605
Nuclear Periphery Foci 0.000390 0.000437 0.021140 0.002123 0.000570 0.000369 0.000990 0.001046 0.000487 0.000435 0.002290 0.001483
Nucleolus 0.000683 0.001857 0.000396 0.000272 0.002398 0.001509 0.000510 0.000171 0.001610 0.001848 0.000503 0.000212
Nucleus 0.107709 0.071901 0.173225* 0.090778 0.147520* 0.056961 0.266755* 0.112312 0.129232 0.071501 0.260721* 0.103571
Peroxisomes 0.001696 0.009236 0.001110 0.002742 0.004252 0.019868 0.001243 0.000653 0.003078 0.009521 0.001234 0.001501
Vacuole 0.032600 0.066009 0.165650 0.161147 0.056059 0.077745 0.064199 0.130178 0.045282 0.066323 0.070745 0.142749
Vacuole Periphery 0.001048 0.002875 0.000589 0.005734 0.001335 0.002305 0.000536 0.001370 0.001203 0.002860 0.000540 0.003141

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.82 -9.21 -2.11 -1.73 -0.13 -1.76 -1.74 -4.05 -1.83 -1.04 -6.12 -2.69 -2.86 -2.03 -1.65
Bud Neck -7.87 6.13 1.95 9.11 -5.76 -2.32 8.24 6.01 2.57 -1.85 -6.06 7.92 6.01 8.81 -2.09
Bud Site -7.53 -5.07 -7.81 -2.81 -5.07 -2.22 -3.28 -7.85 -1.18 -5.48 -7.34 -4.11 -11.07 -3.19 -7.20
Cell Periphery 3.26 -7.64 0.57 -2.38 7.81 -2.78 4.00 6.24 3.51 3.06 2.99 2.14 4.42 1.22 2.50
Cytoplasm 9.96 5.00 11.28 1.44 -0.02 4.39 -8.64 -6.32 -5.77 1.89 5.55 -3.25 2.49 -3.48 5.24
Cytoplasmic Foci -5.33 20.83 36.75 35.95 5.81 -2.80 40.89 40.01 7.68 -1.87 -5.57 53.98 54.34 37.30 -0.76
Eisosomes 2.23 2.06 6.29 10.76 6.53 -3.44 14.27 14.23 5.40 -0.41 4.09 13.32 13.43 12.90 0.00
Endoplasmic Reticulum 0.57 -8.77 -5.69 -7.52 7.30 3.94 -2.78 -1.38 -10.77 2.63 1.49 -6.95 -4.90 -8.11 2.93
Endosome -9.13 -3.37 3.57 10.75 4.81 -2.69 13.05 12.22 3.26 -0.43 -7.89 13.20 11.95 12.49 -0.46
Golgi -5.29 5.04 -0.86 2.22 -2.26 -1.97 4.16 1.66 2.60 -1.57 -5.00 4.95 0.45 4.41 -2.64
Lipid Particles 2.19 2.68 9.81 6.53 4.84 1.46 12.05 12.43 3.58 3.09 5.33 14.97 15.65 7.49 2.65
Mitochondria -2.97 5.45 0.05 2.20 -2.51 -0.18 5.27 4.91 2.09 -0.25 -1.10 6.23 3.90 4.22 -1.69
Mitotic Spindle -1.91 -1.05 -7.15 -6.29 -2.85 2.09 -5.24 -5.91 -6.09 -1.80 -2.60 -4.88 -9.18 -6.64 -4.73
None 2.51 6.55 1.75 -0.96 -5.96 4.87 3.52 3.95 -2.55 1.35 4.14 4.28 4.37 -0.54 0.17
Nuclear Periphery -0.05 -8.32 -15.42 -14.73 -2.96 4.86 -1.18 0.78 -12.17 5.60 4.06 -5.21 -4.88 -17.31 0.84
Nuclear Periphery Foci -0.92 -8.90 -12.67 -11.98 8.06 1.15 -3.99 -4.79 -4.87 -0.12 0.76 -9.06 -11.92 -13.82 3.72
Nucleolus -2.14 4.06 5.97 2.84 1.52 1.66 4.16 4.82 3.37 1.80 -0.38 4.25 5.50 2.98 1.63
Nucleus 7.91 -5.17 -5.10 -11.44 3.42 5.84 -20.30 -1.06 -6.20 18.77 14.18 -25.65 -4.18 -16.85 21.70
Peroxisomes -9.11 1.48 1.51 9.24 -0.28 -2.51 6.37 9.89 3.15 1.63 -7.62 5.35 9.36 10.65 0.76
Vacuole -10.58 -9.75 -30.44 -25.04 -4.57 -0.96 -8.79 -25.22 -6.36 -18.30 -6.79 -14.61 -39.14 -31.11 -24.46
Vacuole Periphery -3.98 2.91 -1.38 0.66 -1.98 -1.91 4.72 1.83 2.71 -0.70 -3.83 4.51 -0.27 2.66 -2.08
Standard name
Human Ortholog
Description Heterotrimeric nascent polypeptide-associated complex beta3 subunit; complex binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3; BTT1 has a paralog, EGD1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (36%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Btt1

Btt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Btt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available