Standard name
Human Ortholog
Description Ribosomal lysine methyltransferase; specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0 0.05 0 0 0.05 0 0.11 0.08 0.13 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.12 0.12 0.18 0.2 0.19 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.98 0.96 0.96 0.91 0.82 0.87 0.85 0.82 0.82 0.83 0.8 0.78 0.87 0.86 0.81 0.93 0.84 0.93 0.95 0.95 0.91 0.79 0.61 0.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.09 0.1 0.1 0.07 0.1 0.19 0.16 0.18 0.15 0 0.07 0.06 0 0 0 0 0 0 0.06 0.11 0.17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0.12 0.24 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 0 0 4 1 5 1 1 0 1 0 0 0 0 0 0 1 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 2 1 5 5 5 5 11 6 7 2 0 0 0 1 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 0 3 6 5 10 6 8 17 7 4 13 8 17 18 30 3 5 1 1 0 1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 3 1 0 0 0 0 0 0
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 0 2 0 1 0 0 0 1 2
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Mitochondria 3 3 2 3 1 25 34 42 60 57 52 62 0 0 1 0 3 1 0 0 1 0 1 3
Nucleus 204 249 188 113 136 244 231 291 269 238 218 204 139 203 187 68 105 64 198 256 183 94 132 97
Nuclear Periphery 0 1 2 3 2 3 11 8 7 4 8 5 1 3 2 1 2 0 0 0 0 0 0 6
Nucleolus 1 5 6 11 16 27 18 34 61 45 48 40 2 16 15 1 3 0 0 1 2 6 24 40
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 1 0 0 0 0 0 1 2 5 2 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 3 8 4 1 4 1 3 6 2 4 3 4 2 5 5 1 11 4 5 7 6 14 51 82
Unique Cell Count 208 260 196 124 166 282 272 356 329 287 271 261 160 236 231 73 125 69 210 271 201 119 216 244
Labelled Cell Count 211 269 209 138 171 312 311 407 412 368 350 332 165 256 243 75 137 71 210 271 201 119 216 244


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.8 11.0 10.2 9.3 9.4 7.8 6.7 6.9 6.5 6.1 5.9 5.9 10.0 10.1 9.5 11.6 13.4 14.4 9.2 10.4 11.0
Std Deviation (1e-4) 2.3 2.4 2.9 2.1 3.3 2.1 2.3 2.3 1.8 2.3 1.7 2.0 2.8 2.7 2.8 2.9 3.5 3.8 1.9 1.8 1.9
Intensity Change (Log2) -0.13 -0.12 -0.4 -0.62 -0.57 -0.65 -0.73 -0.8 -0.79 -0.04 -0.02 -0.11 0.18 0.39 0.5 -0.15 0.03 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.4 0 -0.1 1.0 0 0 0.9 0 2.9 2.1 3.7 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.7 4.6 4.5 5.9 6.2 6.1 6.9 0 0 0 0 0 0
Nucleus -1.8 -4.3 -3.4 -3.8 -4.7 -4.7 -4.3 -4.9 -5.4 -3.1 -3.5 -4.7 -0.9 -3.7 -1.0
Nuclear Periphery 0 0 0 2.0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.3 2.6 2.8 1.7 2.8 5.1 4.4 4.9 4.3 0 1.8 1.6 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 2.8 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.8637 21.9195 28.0944 27.1025 26.4928 34.9032 19.1406 23.3441 26.0271 25.4007
Translational Efficiency 0.9754 0.8967 0.7864 1.1412 0.7704 0.6723 1.1818 0.8257 0.8469 0.7814

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
841 475 427 880 647 1368 187 149 1488 1843 614 1029

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 886.97 1095.79 1269.82 1161.74 950.37 1066.58 1308.84 1306.76 914.54 1074.11 1281.70 1182.74
Standard Deviation 130.60 216.92 172.32 228.48 149.10 209.86 174.88 237.11 142.46 212.09 174.03 235.35
Intensity Change Log 2 0.305014 0.517667 0.389330 0.166431 0.461728 0.459433 0.234995 0.489003 0.426016

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000447 0.000484 0.000475 0.001063 0.000319 0.000601 0.000865 0.000821 0.000391 0.000571 0.000594 0.001028
Bud Neck 0.005593 0.005315 0.008430 0.035589 0.003054 0.007810 0.008951 0.024313 0.004489 0.007167 0.008588 0.033957
Bud Site 0.001734 0.000350 0.000411 0.005474 0.000355 0.000878 0.000544 0.001498 0.001134 0.000742 0.000452 0.004899
Cell Periphery 0.000245 0.000091 0.000049 0.000229 0.000049 0.000157 0.000022 0.000064 0.000160 0.000140 0.000041 0.000205
Cytoplasm 0.001165 0.000114 0.000074 0.002163 0.000909 0.000596 0.000058 0.000765 0.001053 0.000472 0.000069 0.001961
Cytoplasmic Foci 0.001009 0.000071 0.000007 0.001373 0.000013 0.000598 0.000016 0.005491 0.000576 0.000462 0.000010 0.001969
Eisosomes 0.000032 0.000049 0.000055 0.000048 0.000026 0.000072 0.000060 0.000036 0.000030 0.000066 0.000056 0.000046
Endoplasmic Reticulum 0.007570 0.003352 0.002166 0.003167 0.003242 0.005349 0.003669 0.002296 0.005688 0.004834 0.002624 0.003041
Endosome 0.000382 0.000078 0.000028 0.002273 0.000076 0.000642 0.000050 0.006722 0.000249 0.000496 0.000035 0.002918
Golgi 0.000266 0.000081 0.000019 0.002436 0.000045 0.000333 0.000043 0.008151 0.000170 0.000268 0.000026 0.003263
Lipid Particles 0.000466 0.001600 0.000017 0.000738 0.000018 0.000494 0.000040 0.001605 0.000271 0.000779 0.000024 0.000864
Mitochondria 0.002307 0.000854 0.000623 0.004832 0.000385 0.003287 0.001057 0.002106 0.001471 0.002660 0.000755 0.004438
Mitotic Spindle 0.001135 0.000124 0.000006 0.017975 0.000222 0.004639 0.000117 0.005021 0.000738 0.003476 0.000040 0.016099
None 0.003764 0.001187 0.000474 0.002348 0.002718 0.000998 0.000401 0.001908 0.003309 0.001047 0.000451 0.002285
Nuclear Periphery 0.000784 0.000118 0.000049 0.001171 0.000102 0.000525 0.000103 0.000367 0.000488 0.000420 0.000066 0.001055
Nuclear Periphery Foci 0.000361 0.000104 0.000005 0.001301 0.000036 0.000588 0.000012 0.000751 0.000220 0.000463 0.000007 0.001222
Nucleolus 0.013305 0.017384 0.008174 0.017905 0.020896 0.020333 0.022478 0.034652 0.016605 0.019573 0.012530 0.020330
Nucleus 0.956533* 0.967086* 0.978039* 0.889983* 0.966644* 0.949236* 0.959497* 0.899693* 0.960929* 0.953837* 0.972392* 0.891389*
Peroxisomes 0.000454 0.000581 0.000302 0.000783 0.000308 0.000921 0.000942 0.000656 0.000390 0.000833 0.000497 0.000764
Vacuole 0.001875 0.000691 0.000537 0.005671 0.000374 0.001355 0.000966 0.002426 0.001222 0.001184 0.000667 0.005201
Vacuole Periphery 0.000573 0.000289 0.000059 0.003476 0.000208 0.000589 0.000110 0.000656 0.000414 0.000511 0.000074 0.003068

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.09 -0.26 -5.06 -6.63 -6.88 -4.71 -10.52 -6.83 -4.65 -2.73 -2.13 -3.16 -7.63 -6.75 -6.63
Bud Neck 0.28 -2.96 -13.45 -13.65 -12.38 -6.27 -4.69 -7.54 -6.33 -5.53 -3.92 -5.74 -15.50 -14.27 -13.27
Bud Site 1.94 1.83 -3.24 -5.71 -5.63 -3.17 -2.70 -5.68 -4.39 -5.11 0.99 1.61 -4.54 -5.64 -6.00
Cell Periphery 2.47 3.21 0.63 -2.24 -3.20 -3.04 2.47 -1.52 1.81 -2.82 0.47 3.30 -0.47 -0.96 -3.62
Cytoplasm 2.67 2.75 -1.68 -2.77 -2.80 0.42 1.41 0.00 -0.63 -3.27 1.31 2.88 -1.77 -2.39 -2.92
Cytoplasmic Foci 1.53 1.63 0.06 -2.79 -3.01 -2.33 -0.60 -1.35 -0.99 -1.35 0.11 1.63 -1.21 -1.51 -3.07
Eisosomes -3.37 -3.32 -3.07 -0.06 0.83 -5.49 -9.70 -4.76 2.90 3.16 -5.61 -5.83 -4.51 2.44 1.52
Endoplasmic Reticulum 4.73 6.21 4.76 0.04 -3.71 -6.16 -1.75 1.87 6.43 3.01 1.56 5.98 4.56 4.93 -2.11
Endosome 4.03 4.80 -2.79 -3.47 -3.58 -2.62 1.56 -1.44 -1.18 -1.45 -1.50 5.03 -3.27 -2.66 -3.69
Golgi 1.15 1.61 -1.49 -1.69 -1.75 -1.74 -0.22 -1.35 -1.25 -1.35 -0.66 1.63 -2.01 -1.90 -2.17
Lipid Particles -0.69 1.64 -0.17 0.63 -1.59 -2.34 -4.62 -1.16 -0.47 -1.12 -1.07 1.60 -0.92 0.31 -1.86
Mitochondria 1.36 1.57 -1.89 -4.96 -5.36 -2.99 -11.36 -6.13 0.31 -4.36 -1.24 1.14 -3.41 -1.94 -5.62
Mitotic Spindle 1.29 1.45 -4.46 -4.88 -4.91 -2.97 0.25 -1.85 -1.11 -1.86 -2.33 1.53 -4.96 -3.83 -5.24
None 2.81 3.64 1.36 -2.87 -4.80 1.57 2.14 0.16 -2.35 -3.47 3.19 4.10 1.10 -3.73 -5.67
Nuclear Periphery 1.69 1.86 -1.13 -6.00 -6.36 -3.11 -0.42 -4.50 -0.48 -4.56 0.25 1.87 -2.51 -3.91 -6.71
Nuclear Periphery Foci 0.91 1.33 -1.45 -2.33 -2.62 -2.49 1.30 -1.29 0.41 -1.37 -1.20 1.40 -1.99 -1.20 -2.78
Nucleolus -1.76 2.98 -4.14 -2.18 -6.36 0.18 -0.57 -3.77 -3.82 -3.41 -1.92 2.17 -4.73 -3.48 -5.83
Nucleus -2.19 -5.13 9.22 10.99 13.12 4.22 1.93 6.24 5.10 5.55 2.03 -3.55 11.73 10.48 13.62
Peroxisomes -0.47 0.85 -1.16 -0.73 -2.50 -1.52 -3.63 -3.69 -0.48 -0.61 -1.37 -0.96 -2.24 0.08 -1.82
Vacuole 2.55 2.86 -2.91 -4.16 -4.28 -3.24 -4.93 -3.73 -2.14 -2.83 -0.15 1.88 -3.86 -3.82 -4.49
Vacuole Periphery 1.71 3.27 -2.47 -2.86 -3.15 -2.78 0.97 -1.76 0.29 -2.61 -0.80 3.44 -2.71 -2.58 -3.22
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Componentsactinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Ribosomal lysine methyltransferase; specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rkm4

Rkm4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rkm4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available