Standard name
Human Ortholog
Description Ribosomal lysine methyltransferase; specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0 0.05 0 0 0.05 0 0.11 0.08 0.13 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.12 0.12 0.18 0.2 0.19 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.98 0.96 0.96 0.91 0.82 0.87 0.85 0.82 0.82 0.83 0.8 0.78 0.87 0.86 0.81 0.93 0.84 0.93 0.95 0.95 0.91 0.79 0.61 0.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.09 0.1 0.1 0.07 0.1 0.19 0.16 0.18 0.15 0 0.07 0.06 0 0 0 0 0 0 0.06 0.11 0.17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0.12 0.24 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 0 0 4 1 5 1 1 0 1 0 0 0 0 0 0 1 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 2 1 5 5 5 5 11 6 7 2 0 0 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 0 3 6 5 10 6 8 17 7 4 13 8 17 18 30 3 5 1 1 0 1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 3 1 0 0 0 0 0 0
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 0 2 0 1 0 0 0 1 2
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Mitochondria 3 3 2 3 1 25 34 42 60 57 52 62 0 0 1 0 3 1 0 0 1 0 1 3
Nucleus 204 249 188 113 136 244 231 291 269 238 218 204 139 203 187 68 105 64 198 256 183 94 132 97
Nuclear Periphery 0 1 2 3 2 3 11 8 7 4 8 5 1 3 2 1 2 0 0 0 0 0 0 6
Nucleolus 1 5 6 11 16 27 18 34 61 45 48 40 2 16 15 1 3 0 0 1 2 6 24 40
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 1 0 0 0 0 0 1 2 5 2 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 3 8 4 1 4 1 3 6 2 4 3 4 2 5 5 1 11 4 5 7 6 14 51 82
Unique Cell Count 208 260 196 124 166 282 272 356 329 287 271 261 160 236 231 73 125 69 210 271 201 119 216 244
Labelled Cell Count 211 269 209 138 171 312 311 407 412 368 350 332 165 256 243 75 137 71 210 271 201 119 216 244


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.8 11.0 10.2 9.3 9.4 7.8 6.7 6.9 6.5 6.1 5.9 5.9 10.0 10.1 9.5 11.6 13.4 14.4 9.2 10.4 11.0
Std Deviation (1e-4) 2.3 2.4 2.9 2.1 3.3 2.1 2.3 2.3 1.8 2.3 1.7 2.0 2.8 2.7 2.8 2.9 3.5 3.8 1.9 1.8 1.9
Intensity Change (Log2) – – – -0.13 -0.12 -0.4 -0.62 -0.57 -0.65 -0.73 -0.8 -0.79 -0.04 -0.02 -0.11 0.18 0.39 0.5 -0.15 0.03 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.4 0 -0.1 1.0 0 0 0.9 0 2.9 2.1 3.7 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.7 4.6 4.5 5.9 6.2 6.1 6.9 0 0 0 0 0 0
Nucleus -1.8 -4.3 -3.4 -3.8 -4.7 -4.7 -4.3 -4.9 -5.4 -3.1 -3.5 -4.7 -0.9 -3.7 -1.0
Nuclear Periphery 0 0 0 2.0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.3 2.6 2.8 1.7 2.8 5.1 4.4 4.9 4.3 0 1.8 1.6 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 2.8 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – – – – –
Bud Periphery – – – – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – – – – –
Cytoplasmic Foci – – – – – – – – – – – – – – – – – –
Eisosomes – – – – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – – – – –
Lipid Particles – – – – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – – – – –
None – – – – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – – – – –
Punctate Nuclear – – – – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – – – – –
Vacuole Periphery – – – – – – – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.8637 21.9195 28.0944 27.1025 26.4928 34.9032 19.1406 23.3441 26.0271 25.4007
Translational Efficiency 0.9754 0.8967 0.7864 1.1412 0.7704 0.6723 1.1818 0.8257 0.8469 0.7814

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization –
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
841 475 427 880 647 1368 187 149 1488 1843 614 1029

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 886.97 1095.79 1269.82 1161.74 950.37 1066.58 1308.84 1306.76 914.54 1074.11 1281.70 1182.74
Standard Deviation 130.60 216.92 172.32 228.48 149.10 209.86 174.88 237.11 142.46 212.09 174.03 235.35
Intensity Change Log 2 — 0.305014 0.517667 0.389330 — 0.166431 0.461728 0.459433 — 0.234995 0.489003 0.426016

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000447 0.000484 0.000475 0.001063 0.000319 0.000601 0.000865 0.000821 0.000391 0.000571 0.000594 0.001028
Bud Neck 0.005593 0.005315 0.008430 0.035589 0.003054 0.007810 0.008951 0.024313 0.004489 0.007167 0.008588 0.033957
Bud Site 0.001734 0.000350 0.000411 0.005474 0.000355 0.000878 0.000544 0.001498 0.001134 0.000742 0.000452 0.004899
Cell Periphery 0.000245 0.000091 0.000049 0.000229 0.000049 0.000157 0.000022 0.000064 0.000160 0.000140 0.000041 0.000205
Cytoplasm 0.001165 0.000114 0.000074 0.002163 0.000909 0.000596 0.000058 0.000765 0.001053 0.000472 0.000069 0.001961
Cytoplasmic Foci 0.001009 0.000071 0.000007 0.001373 0.000013 0.000598 0.000016 0.005491 0.000576 0.000462 0.000010 0.001969
Eisosomes 0.000032 0.000049 0.000055 0.000048 0.000026 0.000072 0.000060 0.000036 0.000030 0.000066 0.000056 0.000046
Endoplasmic Reticulum 0.007570 0.003352 0.002166 0.003167 0.003242 0.005349 0.003669 0.002296 0.005688 0.004834 0.002624 0.003041
Endosome 0.000382 0.000078 0.000028 0.002273 0.000076 0.000642 0.000050 0.006722 0.000249 0.000496 0.000035 0.002918
Golgi 0.000266 0.000081 0.000019 0.002436 0.000045 0.000333 0.000043 0.008151 0.000170 0.000268 0.000026 0.003263
Lipid Particles 0.000466 0.001600 0.000017 0.000738 0.000018 0.000494 0.000040 0.001605 0.000271 0.000779 0.000024 0.000864
Mitochondria 0.002307 0.000854 0.000623 0.004832 0.000385 0.003287 0.001057 0.002106 0.001471 0.002660 0.000755 0.004438
Mitotic Spindle 0.001135 0.000124 0.000006 0.017975 0.000222 0.004639 0.000117 0.005021 0.000738 0.003476 0.000040 0.016099
None 0.003764 0.001187 0.000474 0.002348 0.002718 0.000998 0.000401 0.001908 0.003309 0.001047 0.000451 0.002285
Nuclear Periphery 0.000784 0.000118 0.000049 0.001171 0.000102 0.000525 0.000103 0.000367 0.000488 0.000420 0.000066 0.001055
Nuclear Periphery Foci 0.000361 0.000104 0.000005 0.001301 0.000036 0.000588 0.000012 0.000751 0.000220 0.000463 0.000007 0.001222
Nucleolus 0.013305 0.017384 0.008174 0.017905 0.020896 0.020333 0.022478 0.034652 0.016605 0.019573 0.012530 0.020330
Nucleus 0.956533* 0.967086* 0.978039* 0.889983* 0.966644* 0.949236* 0.959497* 0.899693* 0.960929* 0.953837* 0.972392* 0.891389*
Peroxisomes 0.000454 0.000581 0.000302 0.000783 0.000308 0.000921 0.000942 0.000656 0.000390 0.000833 0.000497 0.000764
Vacuole 0.001875 0.000691 0.000537 0.005671 0.000374 0.001355 0.000966 0.002426 0.001222 0.001184 0.000667 0.005201
Vacuole Periphery 0.000573 0.000289 0.000059 0.003476 0.000208 0.000589 0.000110 0.000656 0.000414 0.000511 0.000074 0.003068

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.09 -0.26 -5.06 -6.63 -6.88 -4.71 -10.52 -6.83 -4.65 -2.73 -2.13 -3.16 -7.63 -6.75 -6.63
Bud Neck 0.28 -2.96 -13.45 -13.65 -12.38 -6.27 -4.69 -7.54 -6.33 -5.53 -3.92 -5.74 -15.50 -14.27 -13.27
Bud Site 1.94 1.83 -3.24 -5.71 -5.63 -3.17 -2.70 -5.68 -4.39 -5.11 0.99 1.61 -4.54 -5.64 -6.00
Cell Periphery 2.47 3.21 0.63 -2.24 -3.20 -3.04 2.47 -1.52 1.81 -2.82 0.47 3.30 -0.47 -0.96 -3.62
Cytoplasm 2.67 2.75 -1.68 -2.77 -2.80 0.42 1.41 0.00 -0.63 -3.27 1.31 2.88 -1.77 -2.39 -2.92
Cytoplasmic Foci 1.53 1.63 0.06 -2.79 -3.01 -2.33 -0.60 -1.35 -0.99 -1.35 0.11 1.63 -1.21 -1.51 -3.07
Eisosomes -3.37 -3.32 -3.07 -0.06 0.83 -5.49 -9.70 -4.76 2.90 3.16 -5.61 -5.83 -4.51 2.44 1.52
Endoplasmic Reticulum 4.73 6.21 4.76 0.04 -3.71 -6.16 -1.75 1.87 6.43 3.01 1.56 5.98 4.56 4.93 -2.11
Endosome 4.03 4.80 -2.79 -3.47 -3.58 -2.62 1.56 -1.44 -1.18 -1.45 -1.50 5.03 -3.27 -2.66 -3.69
Golgi 1.15 1.61 -1.49 -1.69 -1.75 -1.74 -0.22 -1.35 -1.25 -1.35 -0.66 1.63 -2.01 -1.90 -2.17
Lipid Particles -0.69 1.64 -0.17 0.63 -1.59 -2.34 -4.62 -1.16 -0.47 -1.12 -1.07 1.60 -0.92 0.31 -1.86
Mitochondria 1.36 1.57 -1.89 -4.96 -5.36 -2.99 -11.36 -6.13 0.31 -4.36 -1.24 1.14 -3.41 -1.94 -5.62
Mitotic Spindle 1.29 1.45 -4.46 -4.88 -4.91 -2.97 0.25 -1.85 -1.11 -1.86 -2.33 1.53 -4.96 -3.83 -5.24
None 2.81 3.64 1.36 -2.87 -4.80 1.57 2.14 0.16 -2.35 -3.47 3.19 4.10 1.10 -3.73 -5.67
Nuclear Periphery 1.69 1.86 -1.13 -6.00 -6.36 -3.11 -0.42 -4.50 -0.48 -4.56 0.25 1.87 -2.51 -3.91 -6.71
Nuclear Periphery Foci 0.91 1.33 -1.45 -2.33 -2.62 -2.49 1.30 -1.29 0.41 -1.37 -1.20 1.40 -1.99 -1.20 -2.78
Nucleolus -1.76 2.98 -4.14 -2.18 -6.36 0.18 -0.57 -3.77 -3.82 -3.41 -1.92 2.17 -4.73 -3.48 -5.83
Nucleus -2.19 -5.13 9.22 10.99 13.12 4.22 1.93 6.24 5.10 5.55 2.03 -3.55 11.73 10.48 13.62
Peroxisomes -0.47 0.85 -1.16 -0.73 -2.50 -1.52 -3.63 -3.69 -0.48 -0.61 -1.37 -0.96 -2.24 0.08 -1.82
Vacuole 2.55 2.86 -2.91 -4.16 -4.28 -3.24 -4.93 -3.73 -2.14 -2.83 -0.15 1.88 -3.86 -3.82 -4.49
Vacuole Periphery 1.71 3.27 -2.47 -2.86 -3.15 -2.78 0.97 -1.76 0.29 -2.61 -0.80 3.44 -2.71 -2.58 -3.22
Standard name
Human Ortholog
Description Ribosomal lysine methyltransferase; specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rkm4

Rkm4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rkm4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available