Standard name
Human Ortholog
Description Peroxisomal membrane E3 ubiquitin ligase; required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.19 0.06 0 0 0 0 0 – 0 0 0 0.18 0.07 0.09 0 0.05 0 0.05 0.09 0 0 0.11 0.11
Bud 0 0.08 0.21 0.15 0.2 0.19 0.2 0.2 – 0.2 0.21 0.22 0.07 0.07 0.07 0.08 0.09 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0.08 0.08 0.1 0.11 0.07 0.05 – 0.06 0.07 0.07 0 0 0 0 0.06 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.07 0.06 0 0.06 – 0.06 0 0 0.07 0 0 0 0.06 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.12 0.08 0 0 0 0 0 0.05 – 0 0 0 0.09 0.1 0.19 0.1 0.12 0.17 0.27 0.15 0.16 0.18 0.1 0.13
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.5 0.66 0.55 0.44 0.56 0.62 0.56 0.37 – 0.46 0.66 0.55 0.12 0.14 0.09 0.2 0.08 0.08 0.05 0.09 0 0.06 0.1 0.1
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.42 0.43 0.38 0.52 0.37 0.3 0.3 0.29 – 0.33 0.23 0.32 0.25 0.62 0.63 0.53 0.51 0.5 0.49 0.56 0.68 0.61 0.62 0.55
SpindlePole 0.11 0.05 0.31 0.39 0.28 0.31 0.39 0.48 – 0.42 0.27 0.36 0.4 0.36 0.41 0.38 0.29 0.4 0.05 0.05 0.06 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 24 13 3 6 6 2 3 – 3 8 11 20 25 34 3 6 5 15 14 4 3 28 33
Bud 5 10 47 48 135 165 186 165 – 180 114 247 8 23 26 10 10 8 2 1 1 0 2 1
Bud Neck 2 1 9 5 12 6 12 8 – 7 2 8 5 8 3 2 5 1 4 3 0 3 2 6
Bud Site 5 4 18 27 66 99 64 41 – 54 40 82 3 8 7 4 7 2 – – – – – –
Cell Periphery 0 2 0 3 1 4 2 3 – 1 4 2 1 3 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 2 7 18 48 53 34 50 – 51 22 49 8 6 9 5 7 7 1 0 0 0 0 1
Endoplasmic Reticulum 0 4 1 1 2 0 3 0 – 0 1 0 5 0 0 1 2 0 1 0 0 0 0 0
Endosome 25 10 3 9 17 16 13 38 – 12 1 7 10 33 71 12 13 22 91 23 40 20 25 40
Golgi 1 2 1 0 0 0 0 0 – 0 0 0 1 7 6 5 3 5 15 1 1 0 1 4
Mitochondria 108 83 125 144 374 541 538 299 – 408 360 611 14 46 32 24 9 10 18 13 8 6 25 29
Nucleus 7 0 1 2 4 8 10 14 – 2 3 8 2 0 1 0 1 2 0 0 0 0 1 0
Nuclear Periphery 0 1 0 3 3 0 1 1 – 1 3 0 1 0 2 2 0 1 0 0 1 0 0 1
Nucleolus 19 0 0 4 10 7 14 14 – 18 5 14 0 3 2 3 0 5 0 0 0 0 0 0
Peroxisomes 90 54 85 169 247 263 289 236 – 297 127 349 29 207 233 64 57 67 165 87 169 67 163 169
SpindlePole 24 6 71 127 184 269 367 395 – 371 150 396 46 122 151 45 32 53 16 8 14 3 6 10
Vac/Vac Membrane 1 1 0 0 2 2 5 6 – 4 2 1 2 1 3 3 2 3 2 0 0 1 3 1
Unique Cell Count 214 125 226 326 669 873 953 819 888 546 1105 114 336 372 120 112 133 337 155 248 111 263 307
Labelled Cell Count 296 204 381 563 1111 1439 1540 1273 1409 842 1785 155 492 580 183 154 191 337 155 248 111 263 307


Peroxisomes

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.7 3.3 3.4 3.4 3.4 3.1 3.6 – 3.1 3.0 3.2 5.6 5.5 5.6 6.1 6.0 5.9 5.9 5.7 5.6
Std Deviation (1e-4) 8.9 0.8 0.6 1.0 1.2 1.0 1.1 1.2 – 1.0 1.0 1.0 1.0 1.0 0.7 1.8 1.5 1.6 1.6 1.2 1.4
Intensity Change (Log2) – – – 0.04 0.02 0.04 -0.1 0.09 – -0.09 -0.14 -0.08 0.73 0.71 0.74 0.86 0.85 0.82 0.81 0.78 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0716 -0.2064 -0.2627 -0.0866 -0.7442 -0.166 0.4111 0.6989 0.7785 0.6253 0.243 0.6793 -1.7392 -1.3475 -1.4261 -0.9556 -1.6658 -1.544
Actin 0.0462 0.0073 0.0362 0.0046 0.0156 0.0062 0.0256 0.0272 0.0258 0.0084 0.042 0.028 0.029 0.0647 0.0204 0.0114 0.0046 0.0182
Bud 0.0004 0.0002 0.0045 0.0001 0.0003 0 0.0002 0.0019 0.0009 0.0001 0.0002 0.0001 0.0001 0.0006 0.0015 0.0001 0 0.0001
Bud Neck 0.0042 0.0051 0.0092 0.0017 0.0002 0.0033 0.0011 0.0021 0.0063 0.0028 0.0008 0.0027 0.0013 0.0077 0.0208 0.0215 0.0001 0.0029
Bud Periphery 0.0005 0.0002 0.0036 0.0003 0.0008 0.0001 0.0004 0.005 0.0024 0.0001 0.0003 0.0002 0.0002 0.0005 0.0055 0.0002 0 0.0001
Bud Site 0.0036 0.0169 0.0084 0.0004 0.0017 0.0003 0.0044 0.0157 0.0123 0.0023 0.0007 0.0003 0.0014 0.0311 0.0078 0.0038 0.0001 0.0003
Cell Periphery 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0.0004 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001 0.0003 0.0002 0 0
Cytoplasm 0.0064 0.0226 0.0022 0.0015 0.0003 0.0012 0.0045 0.0158 0.0044 0.0002 0.0019 0.0045 0.0013 0.0107 0.004 0.002 0.0006 0.0069
Cytoplasmic Foci 0.1384 0.2183 0.1212 0.0893 0.0672 0.1838 0.1766 0.2085 0.1244 0.0712 0.1246 0.1616 0.2126 0.2529 0.199 0.2454 0.1966 0.2224
Eisosomes 0.0006 0.0001 0.0006 0.0001 0.0004 0.0003 0.0015 0.0007 0.001 0.0003 0.0003 0.0027 0.0002 0.0004 0.001 0.0002 0 0.0001
Endoplasmic Reticulum 0.0009 0.0004 0.0002 0.0001 0.0001 0.0001 0.0008 0.0008 0.0006 0 0.0003 0.0006 0.0004 0.0001 0.0006 0.0021 0 0.0005
Endosome 0.0286 0.0384 0.0067 0.0122 0.0068 0.0085 0.0205 0.0201 0.0162 0.0213 0.0381 0.0098 0.0242 0.0207 0.0204 0.0175 0.0027 0.0127
Golgi 0.0238 0.019 0.0077 0.0146 0.0088 0.0082 0.0133 0.0105 0.0113 0.0151 0.0125 0.0057 0.0144 0.0175 0.0158 0.0066 0.0063 0.0167
Lipid Particles 0.084 0.0384 0.029 0.0669 0.0444 0.0681 0.0685 0.0157 0.0179 0.0359 0.0539 0.0454 0.1309 0.0466 0.0648 0.0936 0.0369 0.0892
Mitochondria 0.0153 0.0055 0.0191 0.0762 0.0469 0.0081 0.0078 0.0202 0.0392 0.0624 0.0115 0.0116 0.0114 0.0062 0.0202 0.0017 0.002 0.0027
None 0.0205 0.0661 0.0435 0.05 0.0019 0.0327 0.0255 0.12 0.0583 0.0015 0.062 0.0498 0.0164 0.0374 0.0354 0.0078 0.0025 0.0032
Nuclear Periphery 0.0015 0.0003 0.0035 0.0002 0 0.0001 0.0007 0.0004 0.0008 0 0.0002 0.0003 0.0007 0.0001 0.0039 0.0155 0 0.0104
Nucleolus 0.0143 0.0007 0.0003 0.0009 0.0001 0.0003 0.0006 0.0009 0.0007 0.0002 0.0006 0.0007 0.0007 0.0008 0.0033 0.0029 0.0001 0.0003
Nucleus 0.0029 0.0009 0.0004 0.0002 0 0.0001 0.0006 0.0007 0.0006 0.0001 0.0002 0.0002 0.0004 0.0005 0.002 0.0023 0 0.0009
Peroxisomes 0.5973 0.5559 0.6971 0.6774 0.8032 0.6703 0.6367 0.5241 0.6724 0.7766 0.6424 0.6599 0.5477 0.4956 0.5645 0.5517 0.7398 0.5969
Punctate Nuclear 0.0088 0.0025 0.0058 0.0019 0.0006 0.0079 0.0089 0.0076 0.0019 0.0004 0.0065 0.0143 0.005 0.0033 0.0067 0.0117 0.0077 0.0148
Vacuole 0.0011 0.0009 0.0003 0.0008 0.0002 0.0003 0.0013 0.0013 0.0019 0.0006 0.0007 0.0011 0.0014 0.0022 0.0015 0.0008 0 0.0002
Vacuole Periphery 0.0006 0.0002 0.0004 0.0005 0.0001 0.0001 0.0003 0.0003 0.0006 0.0003 0.0004 0.0002 0.0003 0.0003 0.0007 0.0007 0 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.7935 9.7276 8.6619 11.4871 9.1706 5.4378 13.278 17.9235 14.3804 14.5788
Translational Efficiency 0.4313 0.5129 0.5424 0.3835 0.5836 1.3007 0.4716 0.4371 0.5969 0.4652

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1674 1291 294 563 2690 2025 186 150 4364 3316 480 713

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 646.49 685.79 949.85 943.70 737.02 742.41 975.91 945.35 702.29 720.37 959.95 944.05
Standard Deviation 79.35 105.30 116.94 111.13 84.19 91.60 99.88 103.89 93.39 101.01 111.37 109.65
Intensity Change Log 2 — 0.085139 0.555072 0.545700 — 0.010512 0.405044 0.359145 — 0.045865 0.477095 0.449328

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.013816 0.014812 0.038884 0.217676* 0.018036 0.024112 0.049706 0.128235* 0.016417 0.020491 0.043077 0.198859*
Bud Neck 0.002504 0.004588 0.008195 0.001691 0.002755 0.004163 0.003485 0.003054 0.002659 0.004328 0.006370 0.001978
Bud Site 0.003790 0.006368 0.025188 0.015636 0.003975 0.004504 0.016684 0.014131 0.003904 0.005230 0.021892 0.015320
Cell Periphery 0.000125 0.000110 0.000118 0.000133 0.000078 0.000108 0.000129 0.000760 0.000096 0.000109 0.000122 0.000265
Cytoplasm 0.003291 0.010489 0.003988 0.002714 0.004797 0.008589 0.002427 0.001249 0.004219 0.009329 0.003383 0.002406
Cytoplasmic Foci 0.144246 0.160708 0.128622 0.063152 0.107565 0.147265 0.095714 0.077690 0.121636 0.152499 0.115870 0.066211
Eisosomes 0.000134 0.000184 0.000096 0.000175 0.000122 0.000277 0.000125 0.000282 0.000127 0.000240 0.000107 0.000197
Endoplasmic Reticulum 0.000318 0.000101 0.000618 0.000154 0.000014 0.000098 0.000598 0.000057 0.000131 0.000099 0.000610 0.000133
Endosome 0.025193 0.051369 0.042974 0.019334 0.026362 0.044844 0.015933 0.010907 0.025914 0.047384 0.032496 0.017561
Golgi 0.045627 0.068913 0.020448 0.017259 0.047886 0.090443 0.006939 0.012363 0.047020 0.082061 0.015213 0.016229
Lipid Particles 0.017327 0.030962 0.037578 0.007772 0.007155 0.013388 0.020539 0.003912 0.011057 0.020230 0.030976 0.006960
Mitochondria 0.010730 0.010697 0.000962 0.003207 0.009583 0.017092 0.000293 0.000504 0.010023 0.014602 0.000703 0.002638
Mitotic Spindle 0.002305 0.004470 0.126550* 0.008234 0.001931 0.004896 0.027725 0.006251 0.002074 0.004730 0.088255 0.007817
None 0.000167 0.000692 0.000057 0.000018 0.000053 0.000143 0.000036 0.000022 0.000096 0.000357 0.000049 0.000019
Nuclear Periphery 0.000023 0.000013 0.000117 0.000010 0.000004 0.000019 0.000037 0.000003 0.000011 0.000017 0.000086 0.000008
Nuclear Periphery Foci 0.000137 0.000571 0.002813 0.000187 0.000028 0.000134 0.001533 0.000104 0.000070 0.000304 0.002317 0.000170
Nucleolus 0.000178 0.000734 0.000822 0.000048 0.000063 0.000410 0.000389 0.000006 0.000107 0.000536 0.000654 0.000040
Nucleus 0.000255 0.001712 0.006850 0.000053 0.000160 0.000419 0.001310 0.000047 0.000196 0.000922 0.004703 0.000052
Peroxisomes 0.729361* 0.630703* 0.550848* 0.641788* 0.768961* 0.637541* 0.751999* 0.739985* 0.753770* 0.634879* 0.628794* 0.662446*
Vacuole 0.000364 0.001589 0.004126 0.000724 0.000366 0.001029 0.004327 0.000431 0.000365 0.001247 0.004204 0.000662
Vacuole Periphery 0.000111 0.000218 0.000145 0.000035 0.000106 0.000527 0.000072 0.000005 0.000108 0.000407 0.000117 0.000029

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.46 -3.84 -16.31 -16.23 -12.87 -2.79 -3.39 -5.56 -5.25 -3.60 -2.62 -4.94 -16.93 -16.51 -13.05
Bud Neck -1.89 -1.93 0.93 2.39 2.14 -1.65 -0.64 -0.15 0.53 0.19 -2.47 -1.99 0.83 2.65 2.22
Bud Site -2.17 -4.77 -4.93 -3.81 1.94 -0.57 -3.62 -1.91 -1.81 0.41 -1.82 -5.85 -5.25 -4.62 1.79
Cell Periphery 0.48 -0.21 -0.38 -1.10 -0.23 -1.78 -2.56 -1.04 -0.99 -0.95 -0.92 -2.17 -1.23 -1.14 -0.97
Cytoplasm -4.56 -0.81 0.71 4.92 1.09 -2.95 1.32 5.34 6.36 1.53 -5.14 0.09 3.08 7.05 1.34
Cytoplasmic Foci -2.70 1.73 12.29 14.40 6.85 -9.08 1.10 2.79 6.41 1.37 -8.48 0.82 10.93 16.53 6.54
Eisosomes -0.95 1.81 -2.08 0.15 -4.75 -5.07 -0.43 -1.25 -0.05 -1.18 -4.11 1.10 -2.35 1.08 -2.86
Endoplasmic Reticulum 0.76 -1.63 0.56 -1.54 3.02 -2.97 -3.44 -1.44 0.61 3.13 0.27 -3.55 -0.10 -1.29 4.05
Endosome -7.84 -3.17 2.01 8.69 4.07 -6.83 2.49 4.80 9.24 1.20 -10.21 -1.77 3.74 11.31 3.69
Golgi -6.10 8.77 9.54 14.23 0.91 -11.33 19.43 12.05 20.25 -2.39 -12.79 16.60 14.00 23.53 -0.72
Lipid Particles -3.93 -3.68 3.78 6.49 5.32 -4.24 -2.65 2.71 6.24 3.25 -5.63 -5.16 2.39 6.41 5.90
Mitochondria 0.01 5.71 3.42 3.64 -1.50 -4.39 9.38 9.13 11.83 -1.19 -3.37 10.27 5.17 7.84 -1.65
Mitotic Spindle -2.12 -8.99 -3.39 -2.13 8.51 -4.17 -3.38 -0.97 -0.30 2.58 -4.53 -9.36 -3.56 -1.92 8.63
None -1.57 1.53 2.54 2.09 3.08 -1.39 0.76 2.19 1.89 1.60 -1.90 1.31 3.18 2.54 3.53
Nuclear Periphery 0.51 -2.53 0.65 0.80 3.05 -1.87 -2.81 0.41 1.94 2.85 -0.62 -3.30 0.32 1.49 3.50
Nuclear Periphery Foci -2.43 -3.73 -0.45 1.98 3.66 -1.45 -3.18 -1.74 0.33 3.01 -2.83 -4.49 -1.10 1.36 4.31
Nucleolus -2.34 -1.63 1.59 3.28 2.09 -1.39 -1.36 1.55 1.65 1.65 -2.41 -2.14 1.57 2.93 2.48
Nucleus -2.91 -1.62 1.35 3.46 1.67 -1.97 -1.68 1.38 3.02 1.79 -3.43 -1.87 1.91 4.30 1.92
Peroxisomes 9.17 9.66 5.83 -0.78 -4.54 15.25 1.41 1.28 -4.41 0.05 17.65 9.25 7.42 -2.15 -2.48
Vacuole -2.99 -3.37 -1.95 1.72 3.06 -3.41 -2.25 -0.44 1.17 2.15 -4.41 -4.00 -2.04 1.83 3.67
Vacuole Periphery -1.34 -0.91 1.51 2.89 3.08 -2.33 0.52 2.48 2.97 2.18 -2.61 -0.64 2.46 3.43 3.51
Standard name
Human Ortholog
Description Peroxisomal membrane E3 ubiquitin ligase; required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders
Localization
Cell Percentages peroxisome (70%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pex10

Pex10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pex10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available