Standard name
Human Ortholog
Description RING finger ubiquitin ligase (E3); involved in ubiquitination and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.98 0.99 0.95 0.92 0.88 0.89 0.78 0.82 0.6 0.73 1.0 0.99 0.99 0.91 0.94 0.89 0.96 0.93 0.92 0.94 0.94 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.13 0.1 0.19 0.15 0.44 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.05 0.07 0 0.05 0 0 0 0.07 0.05 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 1 2 0 1 1 0 0 0 0 0 0 1 0 1 0 0 0 0
Bud 0 2 0 1 1 0 1 4 3 1 8 2 0 0 0 0 0 0 0 1 1 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 9 11 7 13 10 14 4 7 6 3 9 1 6 6 15 12 11 0 0 0 0 0 0
Cytoplasm 275 447 199 246 315 284 410 401 243 319 150 183 252 270 318 218 198 218 260 441 189 180 296 287
Endoplasmic Reticulum 0 9 3 0 1 4 8 5 9 6 7 6 2 3 0 15 6 6 0 3 3 1 2 0
Endosome 0 1 0 0 1 0 1 1 1 2 2 3 0 1 0 2 1 1 1 4 1 0 2 2
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 3 0 0 1 2
Mitochondria 3 1 0 3 13 20 59 44 60 60 109 60 0 0 0 6 5 4 1 6 2 0 2 1
Nucleus 0 1 2 0 0 2 3 5 2 5 5 4 0 1 1 2 0 5 0 2 0 1 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 1 1 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 3 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 2 1 4 10 11 11 15 15 27 8 12 0 1 1 16 10 28 0 3 3 1 0 0
Unique Cell Count 278 462 204 249 330 310 465 453 310 391 249 250 253 273 320 240 211 244 272 474 207 191 316 301
Labelled Cell Count 281 474 216 262 354 331 509 484 341 428 293 281 255 282 327 275 233 274 272 474 207 191 316 301


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 7.5 8.2 8.3 7.9 6.8 6.3 6.5 5.5 5.8 5.1 5.5 8.8 8.7 8.2 8.5 8.7 8.2 8.0 8.5 8.3
Std Deviation (1e-4) 0.8 1.0 1.1 1.4 1.4 1.4 1.1 1.5 1.1 1.2 1.1 1.3 1.0 1.1 1.2 1.5 1.6 1.6 1.4 1.3 1.3
Intensity Change (Log2) – – – 0.02 -0.05 -0.27 -0.37 -0.33 -0.58 -0.48 -0.67 -0.57 0.1 0.1 0.01 0.06 0.09 0.0 -0.03 0.05 0.02

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.4 -0.8 -1.2 -1.5 0 -1.9 -2.7 -2.6 -0.9 -3.3 -1.9 -2.2 0.4 0.1 -0.4
Cytoplasm 1.0 -1.2 -2.8 -3.9 -3.8 -6.1 -5.5 -9.4 -7.1 1.9 1.1 1.8 -3.0 -1.9 -3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.5 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.7 5.3 4.6 6.7 5.9 10.8 7.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 2.8 3.5 0 2.7 0 0 0 3.4 2.7 4.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3908 4.9353 4.3477 4.3048 3.5552 4.4231 3.1407 5.194 4.0526 3.2019 3.1149 4.3689 3.845 5.3678 4.6153 3.9223 4.2959 4.8772
Actin 0.0415 0.0004 0.0144 0.0061 0.0135 0.0016 0.0098 0.0001 0.0224 0.0018 0.0136 0.0093 0.0773 0.0008 0.0198 0.0197 0.0082 0.0127
Bud 0.0008 0.0037 0.0004 0.0043 0.0004 0.0028 0.0004 0.0001 0.0015 0.0002 0.0112 0.0001 0.0005 0.0023 0.0012 0.0006 0.0005 0.0001
Bud Neck 0.0085 0.0001 0.0015 0.0009 0.0005 0.0009 0.0016 0.0001 0.0006 0.0003 0.0084 0.0015 0.0017 0.0002 0.0017 0.0092 0.003 0.0014
Bud Periphery 0.0008 0.001 0.0004 0.0021 0.0003 0.0017 0.0004 0 0.0062 0.0002 0.0116 0.0001 0.0005 0.0005 0.0004 0.001 0.001 0.0001
Bud Site 0.006 0.0024 0.002 0.0038 0.0007 0.0002 0.001 0.0007 0.0044 0.0003 0.0034 0.0002 0.0021 0.0026 0.0011 0.0109 0.0011 0.0001
Cell Periphery 0.0003 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0 0.0003 0.0002 0.0001 0.0014 0.0002 0
Cytoplasm 0.6963 0.959 0.9019 0.8737 0.8241 0.9433 0.8027 0.9419 0.8769 0.7319 0.7606 0.8994 0.7324 0.9792 0.9183 0.8567 0.9121 0.9529
Cytoplasmic Foci 0.0425 0.004 0.0105 0.0317 0.027 0.0127 0.0248 0.0064 0.0219 0.09 0.0397 0.0126 0.0236 0.0027 0.0151 0.0246 0.0106 0.006
Eisosomes 0.0003 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0001 0.0001 0.0008 0 0.0002 0.0003 0.0001 0
Endoplasmic Reticulum 0.0134 0.0039 0.0037 0.0031 0.0022 0.0022 0.0098 0.0022 0.0025 0.0031 0.0014 0.0044 0.0157 0.0022 0.0027 0.0048 0.0066 0.0028
Endosome 0.058 0.0025 0.0146 0.023 0.0541 0.0104 0.0347 0.005 0.0112 0.0222 0.0191 0.0184 0.0196 0.0012 0.0059 0.0179 0.0229 0.0132
Golgi 0.0202 0.0002 0.0034 0.0092 0.0311 0.0016 0.007 0.0003 0.0039 0.0097 0.0054 0.0049 0.0069 0.0002 0.0021 0.0051 0.0092 0.0014
Lipid Particles 0.0125 0 0.0027 0.0028 0.0062 0.0004 0.0049 0.0001 0.0008 0.0189 0.0185 0.0038 0.014 0 0.004 0.0086 0.0064 0.0009
Mitochondria 0.0154 0.0003 0.0057 0.0028 0.0104 0.0049 0.0018 0.0001 0.0009 0.0017 0.0016 0.0116 0.0022 0.0001 0.0003 0.0024 0.0018 0.0003
None 0.0347 0.0168 0.0176 0.0124 0.0082 0.0102 0.0623 0.0398 0.037 0.0884 0.0469 0.0266 0.0616 0.0059 0.0101 0.0176 0.0021 0.0028
Nuclear Periphery 0.0123 0.0009 0.0013 0.0018 0.0011 0.0007 0.0062 0.0005 0.0007 0.0006 0.0006 0.0006 0.0107 0.0004 0.0013 0.002 0.0033 0.002
Nucleolus 0.002 0.0002 0.0012 0.0005 0.0001 0.0004 0.0008 0 0.0001 0.0001 0.0002 0 0.0012 0 0.0012 0.0008 0.001 0
Nucleus 0.0084 0.0019 0.0024 0.0029 0.0012 0.0023 0.011 0.0007 0.0012 0.001 0.001 0.0009 0.0063 0.0007 0.0019 0.0021 0.0025 0.0009
Peroxisomes 0.0137 0 0.0062 0.0077 0.0115 0.0007 0.0052 0 0.0049 0.0242 0.0431 0.0034 0.012 0 0.0059 0.0053 0.001 0.0003
Punctate Nuclear 0.0052 0.0012 0.0031 0.0068 0.0026 0.0006 0.0077 0.0001 0.0004 0.0031 0.0108 0.0004 0.0071 0.0002 0.0052 0.003 0.0034 0.0013
Vacuole 0.0051 0.0012 0.0052 0.0033 0.003 0.0017 0.0061 0.0016 0.0018 0.0016 0.0024 0.0012 0.0025 0.0005 0.001 0.0054 0.0022 0.0005
Vacuole Periphery 0.0019 0.0002 0.0013 0.0008 0.0018 0.0007 0.0014 0.0002 0.0003 0.0004 0.0003 0.0004 0.001 0.0001 0.0002 0.0006 0.0008 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.7117 17.9808 22.1105 32.2434 30.9221 16.0824 25.1928 27.8716 25.8035 32.9671
Translational Efficiency 1.6621 2.4333 1.8374 1.0035 1.3872 2.3084 1.4487 1.2086 1.5047 1.3776

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2577 1382 243 1795 1907 1493 203 104 4484 2875 446 1899

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 699.10 812.76 1273.91 1128.46 829.56 791.53 1262.82 1081.49 754.58 801.74 1268.86 1125.89
Standard Deviation 79.03 107.59 144.81 149.37 103.50 106.59 142.98 148.06 110.93 107.60 144.09 149.68
Intensity Change Log 2 — 0.217331 0.865693 0.690785 — -0.067702 0.606231 0.382602 — 0.069667 0.730702 0.531746

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000154 0.000183 0.000742 0.002280 0.000103 0.000221 0.000339 0.007819 0.000132 0.000203 0.000559 0.002583
Bud Neck 0.013626 0.007585 0.002155 0.002160 0.002981 0.021978 0.001905 0.002141 0.009099 0.015059 0.002041 0.002159
Bud Site 0.018029 0.012510 0.014134 0.022900 0.005946 0.023641 0.012655 0.038203 0.012890 0.018290 0.013461 0.023738
Cell Periphery 0.000228 0.000065 0.000234 0.000059 0.000069 0.000114 0.000118 0.000112 0.000160 0.000091 0.000181 0.000062
Cytoplasm 0.574602 0.637964 0.701465 0.771579 0.686259 0.451084 0.563715 0.659147 0.622089 0.540917 0.638767 0.765421
Cytoplasmic Foci 0.237224 0.160101 0.075489 0.040772 0.163932 0.204034 0.069746 0.093971 0.206054 0.182916 0.072875 0.043685
Eisosomes 0.000199 0.000036 0.000025 0.000010 0.000032 0.000076 0.000031 0.000022 0.000128 0.000057 0.000028 0.000011
Endoplasmic Reticulum 0.003767 0.000598 0.001633 0.000264 0.001495 0.000965 0.001464 0.001626 0.002800 0.000789 0.001556 0.000339
Endosome 0.008198 0.020097 0.007367 0.003131 0.010379 0.040606 0.012955 0.008279 0.009126 0.030747 0.009910 0.003413
Golgi 0.011369 0.003110 0.000604 0.001895 0.003152 0.009071 0.000404 0.001167 0.007875 0.006206 0.000513 0.001855
Lipid Particles 0.005294 0.001708 0.002428 0.000486 0.002273 0.004359 0.002355 0.001382 0.004009 0.003084 0.002395 0.000535
Mitochondria 0.010778 0.001056 0.000051 0.000233 0.001170 0.007855 0.000030 0.000050 0.006692 0.004587 0.000041 0.000223
Mitotic Spindle 0.000493 0.001162 0.002264 0.032577 0.000584 0.009363 0.004108 0.014675 0.000532 0.005421 0.003103 0.031597
None 0.005508 0.002442 0.003014 0.001663 0.001939 0.002620 0.001018 0.001748 0.003990 0.002534 0.002106 0.001667
Nuclear Periphery 0.000141 0.000114 0.000353 0.000131 0.000130 0.000276 0.000379 0.000100 0.000136 0.000198 0.000365 0.000129
Nuclear Periphery Foci 0.000169 0.000707 0.014324 0.001461 0.000680 0.000336 0.009188 0.006329 0.000386 0.000514 0.011986 0.001727
Nucleolus 0.000707 0.000636 0.000095 0.000156 0.000325 0.001102 0.000126 0.000068 0.000544 0.000878 0.000109 0.000151
Nucleus 0.055984 0.032091 0.043029 0.028033 0.030645 0.035743 0.026320 0.010778 0.045208 0.033988 0.035424 0.027088
Peroxisomes 0.001850 0.002703 0.000330 0.002803 0.000838 0.005313 0.000459 0.018911 0.001419 0.004058 0.000388 0.003685
Vacuole 0.047387 0.112635 0.130008 0.087153 0.085295 0.175159 0.292176 0.132568 0.063509 0.145104 0.203820 0.089640
Vacuole Periphery 0.004294 0.002496 0.000255 0.000254 0.001772 0.006084 0.000510 0.000901 0.003222 0.004359 0.000371 0.000290

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.18 -8.22 -1.59 -1.52 -0.26 -4.34 -6.22 -1.34 -1.29 -1.24 -3.72 -9.52 -1.90 -1.76 -0.96
Bud Neck 6.20 14.96 16.99 7.63 3.74 -12.29 3.47 2.89 12.90 0.09 -6.02 15.14 16.92 14.89 2.95
Bud Site 3.87 1.59 2.32 -1.02 0.62 -10.25 -4.61 -2.58 -0.20 -1.75 -4.38 -0.62 -1.11 2.26 -0.56
Cell Periphery 14.56 -1.62 16.57 3.34 11.14 -3.95 -4.55 -3.04 0.48 0.34 8.04 -2.54 16.14 5.74 11.85
Cytoplasm -7.30 -3.97 -16.72 -6.90 -3.64 25.61 7.98 2.74 -5.31 -2.37 12.67 2.66 -10.60 -19.30 -7.77
Cytoplasmic Foci 13.75 29.49 51.24 25.99 7.69 -6.45 16.42 7.04 10.32 -2.27 5.59 32.99 53.76 38.51 7.74
Eisosomes 19.56 19.91 22.91 14.56 8.27 -12.25 0.16 2.60 12.93 2.05 13.54 18.65 23.46 23.50 10.48
Endoplasmic Reticulum 7.89 4.36 9.37 2.64 7.19 2.00 1.11 1.64 0.54 0.97 7.88 4.49 10.54 5.77 8.53
Endosome -9.34 0.57 17.36 14.48 7.35 -16.03 0.90 4.86 16.93 3.43 -18.55 0.95 20.61 24.85 10.22
Golgi 15.24 26.57 22.07 4.87 -0.89 -7.25 11.60 9.02 10.76 -1.16 3.44 28.23 19.84 10.53 -1.40
Lipid Particles 14.42 5.76 20.70 8.43 6.62 -4.49 0.66 2.91 6.09 1.84 3.23 5.43 21.15 10.01 8.06
Mitochondria 12.05 13.41 13.31 6.79 -3.93 -5.96 3.63 3.63 7.26 0.21 2.91 13.74 13.56 7.83 -5.08
Mitotic Spindle -1.41 -3.92 -6.92 -6.44 -5.36 -5.04 -1.41 -1.66 0.66 -0.95 -5.23 -3.06 -7.04 -4.17 -5.34
None 4.13 2.71 6.84 1.67 0.78 -1.00 4.21 1.50 1.67 -1.39 2.63 3.54 6.98 2.23 0.22
Nuclear Periphery 0.72 -7.02 -3.31 -9.32 5.79 -3.67 -7.05 -1.14 1.52 4.13 -2.27 -9.62 -4.75 -2.69 7.09
Nuclear Periphery Foci -9.31 -11.17 -11.01 -6.28 10.04 3.73 -9.61 -5.03 -5.34 2.25 -2.74 -14.18 -10.51 -9.08 12.27
Nucleolus 0.43 3.83 3.38 3.53 -5.88 -3.60 8.27 12.81 4.72 3.21 -2.14 4.72 4.08 5.43 -4.87
Nucleus 11.87 0.56 8.43 -4.02 2.88 -2.32 0.15 8.21 8.16 4.88 7.22 0.80 4.64 -2.42 1.52
Peroxisomes -2.37 16.93 1.20 2.41 -2.01 -6.45 6.07 -1.29 -0.47 -1.37 -6.59 17.43 -0.43 3.61 -2.39
Vacuole -16.96 -10.26 -25.22 -10.12 -0.05 -15.07 -13.64 -6.00 -2.21 3.68 -22.84 -16.24 -23.03 -5.99 5.53
Vacuole Periphery 5.24 15.75 16.19 9.06 4.87 -7.73 12.19 6.26 9.85 -0.04 -3.11 18.63 19.56 13.41 4.97
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description RING finger ubiquitin ligase (E3); involved in ubiquitination and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hel2

Hel2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hel2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available