Standard name
Human Ortholog
Description Cytoplasmic glyoxalase II; catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; GLO2 has a paralog, GLO4, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.96 0.97 0.95 0.96 0.89 0.82 0.82 0.78 0.82 0.72 0.98 0.99 0.98 0.98 0.93 0.93 0.89 0.9 0.91 0.75 0.86 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0.07 0.05 0.06
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0.06 0.11 0.11 0.18 0.13 0.16 0 0 0 0 0.11 0.09 0.07 0 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 2 0 5 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 0 0 1 0 1 1 1 0 0 0 1 0 0 0 0 0 6 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 3 6 1 2 4 6 2 0 3 3 0 2 0 0 0 0 0 0
Cytoplasm 120 123 43 115 139 150 212 256 132 118 111 147 183 364 455 47 131 199 125 115 75 81 328 299
Endoplasmic Reticulum 1 1 0 0 2 2 6 12 5 2 0 5 1 1 0 1 5 2 0 2 0 0 1 2
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 2
Golgi 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 3
Mitochondria 1 0 1 0 1 0 0 1 6 5 8 12 0 0 0 0 2 3 0 1 0 0 0 0
Nucleus 2 1 0 2 0 5 8 19 4 6 0 6 1 8 4 4 0 2 2 4 1 7 19 22
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 5 10
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 4 6
Vac/Vac Membrane 0 1 1 2 7 6 14 34 17 27 18 33 2 3 6 2 15 20 10 1 1 5 5 9
Unique Cell Count 123 125 45 119 147 156 237 312 160 151 135 203 186 369 462 48 141 215 141 128 83 108 383 376
Labelled Cell Count 124 127 45 119 151 163 244 329 167 163 142 215 190 376 468 57 155 229 141 128 83 108 383 376


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.5 16.8 13.5 14.5 16.5 14.4 14.8 14.6 12.3 13.5 12.4 13.7 15.6 16.9 16.2 14.7 15.9 18.8 15.3 15.8 16.4
Std Deviation (1e-4) 3.4 3.0 3.8 2.9 3.3 3.0 3.1 3.0 2.8 3.2 3.1 3.3 2.9 3.4 3.3 3.7 4.6 7.0 5.5 4.7 5.5
Intensity Change (Log2) – – – 0.1 0.29 0.09 0.13 0.11 -0.14 -0.0 -0.13 0.02 0.2 0.33 0.27 0.13 0.23 0.48 0.17 0.23 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 -0.3 0.2 -1.3 -2.3 -2.2 -2.7 -2.2 -3.3 1.2 1.5 1.4 0.6 -0.6 -0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 2.6 0 2.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.6594 13.9625 12.059 10.7318 10.4917 12.5295 9.7444 13.1979 11.5353 10.5783 10.5952 11.2301 9.1246 12.7396 10.3365 9.8613 10.0617 10.8834
Actin 0.0256 0.0003 0.0232 0.011 0.0019 0.001 0.0075 0.0003 0.0127 0.0159 0.0015 0.0121 0.0506 0.014 0.0214 0.0004 0.0001 0.021
Bud 0.0007 0.0001 0.001 0.0004 0.0002 0.0001 0.0005 0.0001 0.001 0.0012 0.0005 0.0003 0.001 0.0001 0.0008 0.0001 0 0.0001
Bud Neck 0.0008 0.0002 0.001 0.0005 0.0007 0.0016 0.0012 0.0002 0.0007 0.0034 0.0006 0.0022 0.001 0.0002 0.0014 0.0003 0.0003 0.0015
Bud Periphery 0.0008 0 0.0009 0.0003 0.0001 0.0001 0.0006 0 0.0013 0.0006 0.0003 0.0003 0.001 0 0.0005 0 0 0.0001
Bud Site 0.0024 0.0003 0.0021 0.0005 0.0004 0.0001 0.0021 0.0001 0.0071 0.0017 0.0014 0.0011 0.0031 0.0007 0.0026 0.0001 0 0
Cell Periphery 0.0003 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0005 0.0001 0.0001 0.0001 0.0002 0 0.0001 0 0 0
Cytoplasm 0.8414 0.9829 0.832 0.8532 0.8641 0.9482 0.8067 0.9912 0.9115 0.9334 0.8851 0.9356 0.8469 0.9749 0.9046 0.9852 0.9886 0.9207
Cytoplasmic Foci 0.016 0.0011 0.0265 0.0193 0.0068 0.0023 0.0569 0.0005 0.0156 0.0123 0.0113 0.0081 0.0061 0.0014 0.006 0.0012 0.0007 0.0011
Eisosomes 0.0002 0 0.0002 0.0001 0 0 0.0001 0 0.0002 0 0 0.0001 0.0005 0 0.0002 0 0 0.0009
Endoplasmic Reticulum 0.007 0.0016 0.0027 0.0058 0.0144 0.0025 0.0048 0.0002 0.0016 0.0016 0.0021 0.0025 0.0029 0.0002 0.0013 0.002 0.0003 0.0033
Endosome 0.0212 0.0005 0.018 0.0139 0.0254 0.006 0.0288 0 0.0065 0.0034 0.0148 0.0089 0.0117 0.0004 0.0031 0.0005 0.0002 0.0034
Golgi 0.0084 0 0.0183 0.0134 0.0218 0.0024 0.0104 0 0.0058 0.0014 0.0052 0.0045 0.0071 0.0008 0.0017 0 0 0.0024
Lipid Particles 0.0116 0 0.0111 0.0021 0.0054 0.0004 0.0068 0 0.0031 0.0001 0.0113 0.0026 0.0282 0.0003 0.0035 0 0 0.0012
Mitochondria 0.0062 0.0001 0.0209 0.0618 0.0234 0.0108 0.0109 0 0.0068 0.0016 0.0239 0.003 0.0074 0.0005 0.0155 0.0001 0 0.0008
None 0.0087 0.0024 0.0086 0.0054 0.0013 0.0007 0.0072 0.0013 0.0031 0.0038 0.0024 0.0018 0.0036 0.0006 0.0156 0.0017 0.0006 0.011
Nuclear Periphery 0.0058 0.0016 0.0029 0.0016 0.0074 0.0033 0.0114 0.0004 0.0039 0.002 0.0043 0.0018 0.0072 0.0004 0.0016 0.0013 0.0008 0.0114
Nucleolus 0.0006 0 0.0001 0.0001 0.0001 0 0.0007 0 0.0002 0 0.0005 0.0001 0.0005 0 0.0002 0 0 0.0007
Nucleus 0.0206 0.0078 0.0105 0.0062 0.0216 0.0184 0.0175 0.0055 0.0096 0.0137 0.0177 0.01 0.013 0.0041 0.0113 0.0063 0.0077 0.0161
Peroxisomes 0.0153 0 0.0162 0.0017 0.0014 0.0001 0.0119 0 0.0056 0.0023 0.0109 0.0031 0.0034 0.0011 0.004 0 0 0.0003
Punctate Nuclear 0.0035 0.0005 0.0019 0.0012 0.0006 0.0005 0.0041 0.0001 0.0019 0.001 0.0042 0.001 0.0017 0.0001 0.004 0.0004 0.0003 0.0029
Vacuole 0.0021 0.0005 0.0012 0.0009 0.002 0.0008 0.0071 0 0.0011 0.0004 0.0014 0.0006 0.0012 0.0001 0.0003 0.0002 0.0002 0.0005
Vacuole Periphery 0.0008 0 0.0007 0.0004 0.001 0.0006 0.0024 0 0.0004 0.0001 0.0006 0.0002 0.0017 0 0.0002 0.0001 0.0001 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.1052 39.455 44.3596 46.3814 20.8089 25.3977 34.453 37.5319 38.5438 39.3927
Translational Efficiency 3.3105 2.2601 2.0196 2.1517 4.4787 4.6955 3.3798 3.1672 3.5773 2.7738

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1839 578 1888 902 1704 1536 2605 2275 3543 2114 4493 3177

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1236.59 1082.98 1659.27 1775.36 1229.72 1432.29 1633.76 1970.95 1233.29 1336.78 1644.48 1915.42
Standard Deviation 195.47 333.46 299.97 396.18 194.88 250.59 299.76 344.69 195.21 316.65 300.11 370.70
Intensity Change Log 2 — -0.191361 0.424181 0.521744 — 0.219994 0.409866 0.680561 — 0.028359 0.417062 0.603116

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000174 0.000472 0.000258 0.001076 0.000147 0.000157 0.000110 0.000620 0.000161 0.000243 0.000172 0.000750
Bud Neck 0.000497 0.000974 0.001312 0.002520 0.000460 0.000698 0.001550 0.002851 0.000479 0.000773 0.001450 0.002757
Bud Site 0.002609 0.005773 0.002894 0.022512 0.001945 0.005565 0.002612 0.007252 0.002290 0.005622 0.002731 0.011585
Cell Periphery 0.000065 0.000239 0.000070 0.000220 0.000050 0.000046 0.000069 0.000085 0.000058 0.000099 0.000069 0.000124
Cytoplasm 0.847312* 0.799262* 0.638132* 0.593401* 0.847994* 0.843864* 0.721225* 0.731112* 0.847640* 0.831669* 0.686309* 0.692014*
Cytoplasmic Foci 0.029072 0.013774 0.002921 0.023665 0.015209 0.012633 0.003948 0.008182 0.022404 0.012945 0.003517 0.012578
Eisosomes 0.000005 0.000012 0.000001 0.000010 0.000003 0.000003 0.000006 0.000004 0.000004 0.000005 0.000004 0.000005
Endoplasmic Reticulum 0.001476 0.001702 0.000093 0.001044 0.000595 0.000205 0.000599 0.000649 0.001052 0.000614 0.000386 0.000761
Endosome 0.000975 0.001564 0.000071 0.014593 0.000539 0.001163 0.000852 0.002550 0.000765 0.001273 0.000524 0.005969
Golgi 0.000360 0.000685 0.000090 0.019874 0.000373 0.001648 0.000461 0.005879 0.000366 0.001385 0.000305 0.009852
Lipid Particles 0.000409 0.000880 0.000196 0.005785 0.000236 0.000164 0.000413 0.000611 0.000326 0.000360 0.000322 0.002080
Mitochondria 0.000207 0.000262 0.000256 0.003770 0.000364 0.000221 0.000248 0.001526 0.000282 0.000232 0.000251 0.002163
Mitotic Spindle 0.002417 0.003708 0.001716 0.094262 0.003076 0.005863 0.008741 0.039961 0.002734 0.005274 0.005789 0.055378
None 0.005508 0.014961 0.011767 0.010741 0.005805 0.012873 0.009293 0.010932 0.005651 0.013444 0.010333 0.010878
Nuclear Periphery 0.000159 0.000179 0.000159 0.001371 0.000098 0.000090 0.000246 0.000441 0.000130 0.000114 0.000209 0.000705
Nuclear Periphery Foci 0.003272 0.005612 0.000607 0.006946 0.001959 0.002341 0.000851 0.002700 0.002640 0.003235 0.000748 0.003905
Nucleolus 0.000125 0.000787 0.000088 0.000349 0.000178 0.000144 0.000325 0.000314 0.000150 0.000320 0.000225 0.000324
Nucleus 0.080401 0.127675 0.332360* 0.113475 0.108137 0.099977 0.202194* 0.160930* 0.093740 0.107550 0.256891* 0.147457*
Peroxisomes 0.000097 0.000174 0.000080 0.000419 0.000086 0.000290 0.000097 0.000212 0.000091 0.000258 0.000090 0.000271
Vacuole 0.024527 0.019246 0.006870 0.059938 0.012395 0.011600 0.045657 0.017917 0.018692 0.013690 0.029359 0.029847
Vacuole Periphery 0.000334 0.002061 0.000056 0.024031 0.000350 0.000456 0.000503 0.005271 0.000342 0.000895 0.000315 0.010598

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.80 -1.20 -3.70 -0.90 -0.77 -0.46 -0.16 -1.48 -1.30 -1.45 -3.61 -1.18 -3.15 -1.63 -0.81
Bud Neck -2.84 -3.91 -11.72 -6.12 -3.90 -2.07 -9.39 -7.41 -5.93 -0.82 -3.32 -8.92 -10.95 -8.00 -2.62
Bud Site -4.04 -0.38 -5.53 -1.99 -5.01 -3.03 -1.89 -4.99 1.00 -3.09 -3.97 -1.21 -6.93 -0.13 -5.54
Cell Periphery -5.22 -5.59 -10.25 -1.26 -7.19 0.62 -5.23 -7.91 -8.14 -3.72 -4.03 -7.26 -12.20 -5.29 -6.92
Cytoplasm 5.68 39.42 26.85 15.22 0.83 1.79 32.64 27.55 24.66 -4.15 4.68 49.98 38.10 28.46 -4.75
Cytoplasmic Foci 6.65 17.45 8.61 0.73 -8.49 1.29 11.97 9.69 6.73 -3.26 6.95 21.04 15.19 5.99 -8.07
Eisosomes -1.87 4.92 -5.85 0.80 -9.38 -0.28 -4.59 -1.24 -1.08 4.67 -1.22 0.06 -2.43 0.37 -2.55
Endoplasmic Reticulum -0.27 3.88 3.57 3.52 -2.43 4.51 2.02 4.30 0.60 1.71 2.21 4.15 4.66 3.36 1.17
Endosome -0.65 11.24 -1.52 -0.56 -2.97 -1.37 0.65 0.25 1.46 -0.34 -1.09 5.73 -0.72 0.46 -3.04
Golgi -2.08 5.02 -2.16 -1.86 -2.40 -1.55 1.27 -1.62 0.48 -2.18 -1.77 2.59 -2.75 -0.51 -3.30
Lipid Particles -1.19 -2.01 -7.97 -4.45 -7.64 1.05 -6.73 -9.30 -10.73 -3.90 -0.46 -6.33 -11.11 -7.91 -8.66
Mitochondria -0.25 -0.70 -1.78 -1.60 -1.57 0.96 0.78 -0.03 -1.77 -1.59 0.75 0.37 -1.11 -2.38 -2.14
Mitotic Spindle -0.95 -1.07 -5.35 -4.38 -5.06 -1.11 -1.96 -4.92 -3.51 -3.38 -1.70 -2.41 -7.46 -5.38 -6.04
None -3.82 -8.27 -1.05 3.46 7.50 -6.21 -4.74 -7.77 2.06 -2.36 -7.25 -8.76 -7.20 3.90 2.05
Nuclear Periphery -0.49 -7.14 -7.01 -5.92 -4.90 -0.01 -17.93 -9.96 -8.92 -0.43 0.45 -16.77 -11.42 -10.68 -3.74
Nuclear Periphery Foci -2.99 10.76 1.36 3.51 -5.35 -0.54 5.80 2.37 1.68 -1.54 -1.05 12.03 4.12 3.12 -4.17
Nucleolus -1.94 0.72 -2.13 1.76 -3.61 0.78 -0.82 -0.50 -2.83 0.58 -1.57 -0.90 -1.52 1.01 -0.65
Nucleus -6.60 -51.70 -12.72 -1.91 31.18 1.85 -29.02 -19.10 -21.82 9.68 -4.66 -55.95 -26.23 -18.17 27.24
Peroxisomes -1.39 0.89 -0.37 1.21 -1.01 -2.01 1.03 0.84 2.83 -0.37 -2.52 1.48 0.98 3.07 -0.79
Vacuole -0.62 3.23 -20.89 -19.12 -21.93 -3.35 -19.27 -20.85 -17.87 -1.34 -1.11 -14.47 -27.21 -25.30 -16.67
Vacuole Periphery -0.99 2.52 -2.09 -0.59 -2.32 -0.11 0.67 -1.38 -1.34 -1.76 -0.99 1.62 -2.53 -1.16 -2.99
Standard name
Human Ortholog
Description Cytoplasmic glyoxalase II; catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; GLO2 has a paralog, GLO4, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Glo2

Glo2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Glo2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available