Standard name
Human Ortholog
Description Ribonuclease H2 subunit; required for RNase H2 activity; role in ribonucleotide excision repair; related to human AGS2 that causes Aicardi-Goutieres syndrome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.09 0.09 0.08 0.12 0.16 0.25 0.16 0.25 0.27 0.23 0.09 0.24 0.19 0.06 0.07 0 0 0 0 0.07 0.05 0.06
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.41 0 0.09 0.12 0.09 0.39 0.64 0.49 0.78 0.56 0.72 0.64 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.88 0.93 0.87 0.88 0.87 0.72 0.53 0.46 0.38 0.47 0.34 0.4 0.88 0.75 0.79 0.85 0.87 0.87 0.86 0.87 0.8 0.72 0.64 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.07 0 0 0 0.06 0.05 0 0 0 0 0.05 0 0 0 0.1 0.06 0.09 0.06 0.07 0.09 0.14 0.19 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 1 1 0 1 7 6 2 5 2 1 0 0 0 0 0 0 2 3 1 0 2 3
Bud Neck 0 0 0 0 0 0 2 6 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Bud Site 3 0 0 2 0 3 3 3 9 9 6 8 0 0 0 0 0 2 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 13 7 14 13 11 28 51 64 32 52 51 40 16 30 36 14 15 8 2 2 2 10 11 11
Endoplasmic Reticulum 0 1 1 0 0 0 0 0 3 0 0 0 0 0 0 14 4 6 0 0 2 0 1 0
Endosome 0 0 0 1 1 4 1 1 0 0 0 0 0 1 2 4 2 3 2 1 0 1 4 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 1 0 0 0
Mitochondria 123 3 14 16 13 90 201 127 151 119 136 114 6 1 1 6 7 7 4 1 2 1 5 6
Nucleus 266 193 140 123 125 165 167 117 73 99 65 71 156 94 148 213 198 212 262 183 138 104 157 120
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 2 0 0 0 0 2 0 2 1 0 1 0 1 2
Nucleolus 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1 5 5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 7 1 1 0 1 1 2 4 2 1 0 2 1 1 2 3 2
Vac/Vac Membrane 1 14 2 1 3 14 15 5 3 5 6 9 1 5 6 24 14 22 18 14 15 20 47 46
Unique Cell Count 302 208 161 139 143 230 313 257 194 212 189 177 177 125 188 250 228 244 304 211 172 145 246 207
Labelled Cell Count 409 218 173 157 153 305 448 337 274 291 269 244 180 133 197 282 243 264 304 211 172 145 246 207


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 5.5 5.3 5.5 5.1 3.9 3.6 4.2 3.2 3.6 3.2 3.4 5.7 6.1 5.6 7.3 7.2 6.9 5.2 5.7 6.0
Std Deviation (1e-4) 0.5 0.8 1.5 2.3 1.4 0.9 1.3 1.5 0.8 1.2 1.1 0.9 1.2 1.6 1.4 1.3 1.6 1.5 1.1 1.5 1.9
Intensity Change (Log2) – – – 0.04 -0.05 -0.44 -0.55 -0.35 -0.75 -0.57 -0.73 -0.64 0.1 0.21 0.09 0.47 0.45 0.39 -0.04 0.11 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 -0.3 1.1 2.3 4.1 2.2 4.0 4.4 3.5 0.1 3.6 2.8 -1.2 -0.8 -2.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.6 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.8 0.1 0 0 0 0 0 0 0 -2.1 -3.0 -3.7 -2.9 -2.4 -2.6
Nucleus 0.4 0.1 -3.6 -7.3 -8.5 -9.4 -8.0 -9.9 -8.9 0.3 -2.6 -2.0 -0.5 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.4 2.0 0 0 0 0 2.0 0 0 0 3.4 2.4 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9852 2.0992 2.1312 1.7066 1.5982 1.8914 1.0538 0.9837 1.2543 1.0938 1.1043 1.1172 1.0949 0.7129 0.5075 0.2345 0.4088 0.4139
Actin 0.0027 0 0 0.0001 0.0004 0.0011 0.0048 0 0.0138 0.0004 0.0027 0.0003 0.0114 0 0.0028 0.0015 0.0199 0.0091
Bud 0.0001 0 0 0.0001 0 0 0.0001 0 0.0003 0 0.0001 0 0.001 0 0.0001 0.0001 0.0007 0.0009
Bud Neck 0.0019 0.0001 0 0.0032 0.0004 0.0004 0.0014 0 0.0012 0.0001 0.0027 0.0005 0.0085 0.0001 0.0001 0.0003 0.0005 0.0065
Bud Periphery 0.0003 0 0 0.0002 0.0001 0.0001 0.0003 0 0.0004 0 0.0002 0 0.0027 0 0.0001 0.0001 0.0009 0.003
Bud Site 0.0007 0 0 0.0004 0.0001 0 0.0015 0 0.0013 0 0.0018 0.0001 0.0104 0 0.0002 0.0003 0.0008 0.0006
Cell Periphery 0.0001 0 0 0.0001 0 0 0.0001 0 0.0002 0 0.0001 0 0.0011 0 0 0 0.0001 0.0001
Cytoplasm 0.0007 0.0065 0.0001 0.0009 0.0004 0.0002 0.0011 0 0.0009 0.0013 0.0006 0.0001 0.0227 0.0007 0.0001 0.0023 0.0004 0.0007
Cytoplasmic Foci 0.0008 0 0 0.0013 0.0022 0.0003 0.0024 0 0.0017 0.0002 0.005 0.0003 0.0334 0 0.0019 0.011 0.0021 0.0021
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0002 0 0 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0003 0.0002 0 0.0002 0.0001 0.0001 0.0003 0 0.0008 0.0007 0.0015 0 0.0212 0 0 0.0005 0.0005 0.0001
Endosome 0.0016 0.0004 0.0001 0.001 0.0043 0.0008 0.0016 0 0.0032 0.0021 0.0201 0.0006 0.0405 0 0.0029 0.0604 0.0143 0.0017
Golgi 0.0004 0 0 0.0001 0.0043 0.0005 0.0009 0 0.0024 0.0001 0.0044 0.0003 0.0073 0 0.0076 0.0051 0.0296 0.0016
Lipid Particles 0.0007 0 0 0.0005 0.0035 0.0013 0.0027 0 0.004 0.0002 0.0082 0.001 0.0198 0 0.0001 0.0035 0.0007 0.0011
Mitochondria 0.0021 0.0001 0.0002 0.0005 0.0139 0.0017 0.0011 0.0001 0.0041 0.0005 0.0025 0.0033 0.0102 0.0003 0.0009 0.0891 0.0052 0.0494
None 0.002 0.0001 0.0004 0.0007 0.0002 0.0005 0.0055 0 0.0002 0.0342 0.001 0.0001 0.0071 0.0001 0 0.0012 0 0.0007
Nuclear Periphery 0.0027 0.0044 0.0046 0.0007 0.004 0.0056 0.0018 0.0011 0.0069 0.0339 0.0209 0.0009 0.0379 0.0038 0.0005 0.0012 0.0004 0.0004
Nucleolus 0.0083 0.0052 0.0062 0.0144 0.02 0.0167 0.0073 0.0052 0.0053 0.0082 0.0133 0.0174 0.0059 0.0049 0.0053 0.0048 0.0308 0.0161
Nucleus 0.97 0.9782 0.9874 0.969 0.9181 0.9627 0.9514 0.9926 0.9451 0.9036 0.9057 0.9672 0.7018 0.9895 0.9767 0.8127 0.8913 0.89
Peroxisomes 0.0004 0 0 0.0005 0.0151 0.0017 0.0064 0 0.0038 0 0.0016 0.0003 0.0154 0 0.0003 0.0011 0.0002 0.0141
Punctate Nuclear 0.0026 0.0037 0.0007 0.0053 0.0126 0.0058 0.0085 0.0009 0.0025 0.0132 0.0038 0.0072 0.0197 0.0003 0.0002 0.0008 0.0002 0.0005
Vacuole 0.0008 0.0009 0 0.0006 0.0001 0.0001 0.0005 0 0.0012 0.0004 0.0023 0.0001 0.0154 0.0002 0.0001 0.0026 0.0006 0.0008
Vacuole Periphery 0.0005 0.0001 0.0001 0.0001 0.0002 0.0002 0.0003 0 0.0004 0.0008 0.0014 0.0001 0.0065 0.0001 0.0001 0.0011 0.0005 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.819 13.1142 7.646 22.1233 25.1342 8.3783 16.1011 20.7755 17.852 31.4761
Translational Efficiency 0.8605 1.252 1.8562 0.6722 0.8668 3.8956 0.8667 0.6129 0.9283 0.8183

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1946 155 1835 1293 1614 1918 967 1615 3560 2073 2802 2908

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 715.12 825.15 855.51 961.41 694.01 787.84 984.44 977.04 705.55 790.63 900.01 970.09
Standard Deviation 83.84 123.91 144.47 133.55 82.60 102.92 124.06 141.47 83.94 105.09 150.79 138.23
Intensity Change Log 2 — 0.206471 0.258599 0.426966 — 0.182946 0.504347 0.493461 — 0.194933 0.384862 0.460099

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000051 0.000443 0.000358 0.000602 0.000062 0.000188 0.000662 0.001083 0.000056 0.000207 0.000463 0.000869
Bud Neck 0.002265 0.002546 0.008819 0.018287 0.001825 0.006863 0.005909 0.027520 0.002065 0.006541 0.007815 0.023414
Bud Site 0.000755 0.001148 0.001407 0.003523 0.001329 0.002918 0.000909 0.010167 0.001015 0.002786 0.001235 0.007213
Cell Periphery 0.000090 0.000123 0.000135 0.000104 0.000127 0.000212 0.000050 0.000135 0.000107 0.000205 0.000106 0.000121
Cytoplasm 0.042673 0.006668 0.005264 0.003670 0.033885 0.012952 0.001283 0.010268 0.038689 0.012482 0.003890 0.007334
Cytoplasmic Foci 0.003524 0.000426 0.000074 0.000856 0.003010 0.001962 0.000149 0.001372 0.003291 0.001847 0.000100 0.001143
Eisosomes 0.000012 0.000019 0.000017 0.000022 0.000013 0.000015 0.000023 0.000023 0.000012 0.000015 0.000019 0.000022
Endoplasmic Reticulum 0.001333 0.005658 0.004277 0.008475 0.001607 0.005196 0.006678 0.005716 0.001457 0.005231 0.005106 0.006943
Endosome 0.000498 0.000300 0.000263 0.001643 0.000352 0.001078 0.000233 0.003409 0.000431 0.001020 0.000253 0.002624
Golgi 0.000195 0.000064 0.000034 0.000792 0.000046 0.000858 0.000067 0.004698 0.000128 0.000798 0.000046 0.002962
Lipid Particles 0.000641 0.000324 0.000078 0.000144 0.000631 0.000630 0.000169 0.000269 0.000636 0.000607 0.000109 0.000214
Mitochondria 0.002571 0.003617 0.000703 0.002603 0.001189 0.004568 0.000666 0.003842 0.001945 0.004497 0.000690 0.003291
Mitotic Spindle 0.000979 0.003510 0.000933 0.008934 0.000475 0.005711 0.001313 0.022119 0.000751 0.005546 0.001064 0.016257
None 0.007536 0.003409 0.003363 0.002193 0.006592 0.005583 0.001192 0.004017 0.007108 0.005420 0.002614 0.003206
Nuclear Periphery 0.000711 0.000366 0.000475 0.001372 0.000907 0.002079 0.000432 0.001950 0.000800 0.001951 0.000460 0.001693
Nuclear Periphery Foci 0.000234 0.000138 0.000178 0.000821 0.000147 0.000407 0.000253 0.001006 0.000195 0.000387 0.000204 0.000924
Nucleolus 0.002711 0.003178 0.001689 0.001106 0.002989 0.003522 0.001832 0.001431 0.002837 0.003496 0.001739 0.001286
Nucleus 0.931178* 0.966375* 0.970286* 0.936486* 0.943131* 0.938853* 0.975991* 0.891653* 0.936597* 0.940911* 0.972255* 0.911587*
Peroxisomes 0.000547 0.000669 0.000067 0.000383 0.000473 0.001316 0.000142 0.000194 0.000514 0.001267 0.000093 0.000278
Vacuole 0.001318 0.000927 0.001476 0.005765 0.001056 0.004329 0.001958 0.007211 0.001199 0.004075 0.001642 0.006568
Vacuole Periphery 0.000178 0.000092 0.000102 0.002219 0.000151 0.000762 0.000090 0.001918 0.000166 0.000712 0.000098 0.002052

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.39 -16.53 -17.48 -1.45 -8.70 -4.99 -3.74 -3.41 -2.92 -0.69 -6.26 -7.00 -5.56 -4.41 -2.32
Bud Neck -0.93 -7.91 -11.96 -10.86 -6.31 -5.74 -8.01 -13.64 -10.31 -10.43 -5.63 -10.47 -17.83 -12.49 -11.48
Bud Site -0.96 -2.25 -4.42 -1.67 -3.15 -2.22 0.38 -4.71 -3.45 -5.24 -3.39 -1.03 -6.17 -3.26 -5.92
Cell Periphery -0.81 -1.78 -0.27 0.73 1.47 -2.55 5.10 0.75 3.05 -5.36 -3.38 0.18 0.15 3.42 -0.08
Cytoplasm 9.67 14.66 14.71 1.22 0.71 8.40 14.58 9.62 1.54 -10.10 13.09 20.05 17.05 3.80 -6.14
Cytoplasmic Foci 6.22 7.85 6.11 0.09 -1.61 1.77 6.07 4.47 2.99 -2.81 3.25 9.95 7.55 3.36 -3.30
Eisosomes -2.35 -4.27 -11.77 -0.56 -6.32 -1.72 -8.97 -9.97 -7.78 -0.91 -3.03 -7.97 -15.20 -8.61 -5.97
Endoplasmic Reticulum -4.80 -16.08 -17.22 -2.25 -8.80 -9.03 -16.14 -15.09 -2.60 1.75 -10.39 -22.37 -23.03 -4.73 -6.29
Endosome 0.51 1.21 -1.47 -1.77 -2.17 -3.90 0.95 -3.64 -1.99 -3.92 -3.34 1.47 -3.75 -1.74 -4.44
Golgi 1.78 2.40 -1.08 -1.61 -1.74 -2.86 -0.59 -2.71 -1.70 -2.68 -2.54 2.15 -2.85 -1.35 -3.02
Lipid Particles 0.69 5.17 4.31 1.60 -2.58 -0.01 4.17 3.44 3.16 -1.11 0.24 6.86 5.34 3.80 -2.81
Mitochondria -0.36 2.50 -0.05 0.35 -4.25 -3.84 1.40 -4.23 1.44 -6.28 -2.87 2.82 -2.04 1.82 -7.53
Mitotic Spindle -1.02 -0.41 -3.75 -1.01 -3.56 -3.92 -1.23 -7.42 -5.02 -6.79 -3.85 -1.22 -8.25 -4.46 -7.77
None 2.69 4.26 4.92 0.86 0.98 0.83 6.30 2.93 1.77 -6.59 1.63 6.61 5.51 2.42 -1.97
Nuclear Periphery 3.03 1.57 -6.82 -8.93 -8.34 -2.26 2.44 -4.66 -0.55 -6.31 -2.46 2.85 -7.04 -0.32 -8.86
Nuclear Periphery Foci 0.36 0.60 -1.14 -1.32 -1.45 -2.01 -0.72 -2.35 -1.26 -1.74 -1.42 -0.04 -2.44 -1.34 -2.41
Nucleolus -0.65 1.87 2.24 2.45 0.41 -0.55 1.85 1.37 2.48 -0.81 -1.09 2.60 2.33 3.22 -0.41
Nucleus -2.97 -9.10 -0.72 2.56 7.62 0.91 -7.54 7.72 6.62 13.83 -0.84 -11.54 5.59 5.45 15.39
Peroxisomes -0.29 2.95 1.36 1.06 -1.24 -1.85 1.86 1.87 2.83 -0.16 -1.89 3.50 2.38 2.77 -1.47
Vacuole -0.72 -1.26 -6.49 -2.36 -5.49 -5.69 -4.87 -8.08 -4.60 -6.68 -5.40 -2.97 -10.18 -4.92 -8.58
Vacuole Periphery 1.52 1.28 -1.99 -2.14 -2.12 -4.17 1.41 -2.82 -0.93 -3.00 -4.04 1.75 -3.26 -1.43 -3.47
Standard name
Human Ortholog
Description Ribonuclease H2 subunit; required for RNase H2 activity; role in ribonucleotide excision repair; related to human AGS2 that causes Aicardi-Goutieres syndrome
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rnh202

Rnh202


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rnh202-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available