Standard name
Human Ortholog
Description 3'-5' DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; acts with Rad4p in nucleotide-excision repair; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome (RTS)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.15 0.06 0.18 – 0.29 0.34 0.26 0.26 0.22 – 0.11 0.19 0.27 0.3 0.38 0.17 0.1 0.17 0.05 0 0.14 0.1 0.15 0.1
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.48 0.55 – 0.39 0.57 0.61 0.67 0.74 – 0.82 0.77 0.15 0.1 0.26 0.09 0.05 0.11 0 0 0.06 0 0 0
Nucleus 0.75 0.55 0.5 – 0.38 0.33 0.33 0.31 0.36 – 0.34 0.36 0.53 0.55 0.34 0.58 0.66 0.61 0.69 0.73 0.35 0.55 0.4 0.36
Nuclear Periphery 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.18 0.29 0 – 0.15 0.05 0.1 0.05 0 – 0 0 0.17 0.15 0.18 0.23 0.2 0.2 0.2 0.18 0.36 0.27 0.32 0.36
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 2 1
Bud 0 0 1 – 1 0 3 4 5 – 3 2 0 0 1 0 0 1 1 1 0 3 5 6
Bud Neck 0 0 0 – 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 – 0 2 3 8 3 – 4 8 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 1 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 25 6 4 – 34 49 61 63 36 – 17 40 61 82 105 25 12 25 9 4 10 17 45 31
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 – 0 0 1 0 0 8 5 9 0 0 0 0 0 2
Endosome 1 0 0 – 1 0 1 0 0 – 0 0 0 4 1 1 4 3 1 1 0 1 2 5
Golgi 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 2 0 1 0 0 0 0 0 2
Mitochondria 1 52 12 – 46 83 142 165 119 – 126 162 34 28 73 13 6 16 3 1 4 1 8 13
Nucleus 123 60 11 – 45 48 76 77 58 – 52 75 118 151 93 87 81 92 129 89 27 98 117 121
Nuclear Periphery 0 0 0 – 0 0 0 0 1 – 1 1 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 – 0 0 0 0 1 – 2 0 0 0 0 0 1 0 0 0 0 0 0 2
Peroxisomes 0 0 0 – 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 1 1 0 0 1 – 1 3 1 1 0 1 1 0 1 0 2 5 10 15
Vac/Vac Membrane 30 32 0 – 17 7 22 12 7 – 4 9 39 42 49 35 25 30 37 21 29 48 95 120
Unique Cell Count 163 109 22 117 146 231 245 161 153 210 224 276 276 149 122 151 187 122 80 181 297 334
Labelled Cell Count 180 151 28 145 192 308 329 231 211 300 254 308 324 172 135 177 187 122 80 181 297 334


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 3.6 3.7 – 3.8 3.6 3.6 3.4 3.3 – 2.8 2.7 4.1 4.4 3.8 5.8 6.6 6.5 4.6 4.1 5.0
Std Deviation (1e-4) 0.5 0.6 1.4 – 1.5 1.7 1.9 1.7 2.3 – 1.2 1.2 0.8 0.9 0.9 1.6 1.8 1.9 1.5 1.5 1.4
Intensity Change (Log2) – – – – 0.03 -0.06 -0.05 -0.12 -0.19 – -0.4 -0.47 0.13 0.22 0.03 0.64 0.82 0.79 0.3 0.14 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0433 0.9713 0.8503 1.0191 0.4533 1.0516 0.9538 1.7275 1.3957 1.3917 1.8515 1.3543 0.7858 2.1858 1.3144 2.2363 1.0714 1.2594
Actin 0.0729 0.0002 0.0051 0.0002 0.0118 0.0038 0.0316 0.0005 0.0065 0.0087 0.0016 0.0058 0.0011 0.0001 0.0001 0.0002 0.0001 0.0001
Bud 0.0013 0.0003 0.0002 0.0004 0.0008 0.0001 0.0009 0.0001 0.0003 0.0002 0.0001 0.0001 0.0002 0 0 0.0001 0 0
Bud Neck 0.0025 0.0009 0.0012 0.0005 0.0021 0.0214 0.0034 0.0003 0.0009 0.0007 0.0008 0.0048 0.0004 0.0001 0.0002 0.0005 0.0004 0.0023
Bud Periphery 0.0017 0.0003 0.0005 0.0003 0.0018 0.0003 0.0011 0 0.0007 0.0003 0.0002 0.0003 0.0002 0 0 0.0001 0 0
Bud Site 0.0045 0.0024 0.0011 0.0003 0.0019 0.0004 0.006 0.0121 0.0092 0.001 0.0002 0.0002 0.001 0.0002 0.0002 0.0002 0.0001 0.0001
Cell Periphery 0.0008 0.0001 0.0004 0 0.0007 0.0001 0.0002 0.0001 0.0004 0 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.053 0.0849 0.048 0.0328 0.0318 0.0377 0.0334 0.0423 0.034 0.0235 0.0212 0.0127 0.0219 0.0541 0.0396 0.0281 0.0365 0.0192
Cytoplasmic Foci 0.0428 0.0083 0.0041 0.0093 0.0092 0.0147 0.0229 0.001 0.0059 0.0301 0.0181 0.0029 0.0064 0.0003 0.0007 0.0008 0.0004 0.0006
Eisosomes 0.0005 0.0001 0.0001 0 0.0004 0.0001 0.0004 0.0001 0.0003 0 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0067 0.0031 0.0046 0.0011 0.0021 0.0023 0.0033 0.0012 0.0014 0.0008 0.0013 0.0006 0.0011 0.0069 0.0011 0.0007 0.0019 0.0007
Endosome 0.0184 0.0078 0.0065 0.0545 0.0214 0.0052 0.0145 0.0006 0.0056 0.0299 0.0258 0.0042 0.0146 0.0011 0.0008 0.0011 0.0007 0.0008
Golgi 0.0077 0.0017 0.004 0.0098 0.0143 0.0019 0.0077 0.0001 0.0025 0.0045 0.0097 0.0045 0.002 0 0 0 0 0
Lipid Particles 0.0276 0.0024 0.0068 0.0003 0.0344 0.004 0.0295 0.0004 0.0076 0.0043 0.0626 0.0113 0.0107 0.0001 0.0001 0.0002 0 0.0001
Mitochondria 0.0067 0.0011 0.0134 0.007 0.0869 0.0097 0.0094 0.0003 0.0041 0.0024 0.0064 0.0106 0.0008 0.0004 0.0004 0.0011 0.0003 0.0002
None 0.0469 0.1025 0.0469 0.0945 0.0604 0.0168 0.0349 0.1188 0.0626 0.0182 0.0571 0.0328 0.0796 0.1439 0.2055 0.2207 0.1589 0.0872
Nuclear Periphery 0.0379 0.0156 0.0183 0.0081 0.0648 0.0342 0.0129 0.009 0.0134 0.0123 0.046 0.0087 0.0052 0.0346 0.0092 0.0146 0.0089 0.0046
Nucleolus 0.0107 0.005 0.0035 0.0055 0.0274 0.0088 0.0044 0.0032 0.0046 0.0032 0.0042 0.0067 0.004 0.0016 0.0026 0.0074 0.0058 0.0069
Nucleus 0.5951 0.7509 0.8121 0.6996 0.5858 0.7992 0.7163 0.7987 0.8194 0.8208 0.6461 0.8613 0.835 0.7488 0.7318 0.6534 0.7724 0.8626
Peroxisomes 0.0197 0.0008 0.0117 0.0002 0.0057 0.0037 0.0312 0.0001 0.0084 0.0196 0.0545 0.0076 0.0014 0 0 0.0001 0 0
Punctate Nuclear 0.0261 0.0084 0.0058 0.07 0.0284 0.0328 0.0324 0.0106 0.0091 0.0132 0.0403 0.0238 0.0119 0.0055 0.0063 0.0695 0.0128 0.014
Vacuole 0.012 0.0029 0.004 0.0027 0.0043 0.0014 0.0026 0.0005 0.0026 0.005 0.0015 0.0004 0.0019 0.0017 0.0009 0.0008 0.0005 0.0004
Vacuole Periphery 0.0045 0.0004 0.0016 0.0027 0.0037 0.0012 0.0008 0.0001 0.0005 0.0013 0.0021 0.0004 0.0005 0.0004 0.0002 0.0003 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.1358 13.4201 13.5795 15.1425 10.838 8.184 15.4194 14.0495 14.8847 16.1258
Translational Efficiency 0.355 0.3826 0.3403 0.3285 0.4549 0.4986 0.3194 0.3159 0.3465 0.334

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1241 1542 304 1221 1895 1800 1280 384 3136 3342 1584 1605

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 623.03 742.36 993.29 870.64 708.96 738.58 881.29 850.13 674.96 740.32 902.78 865.73
Standard Deviation 181.96 94.17 123.94 118.78 87.43 101.10 105.10 123.68 139.60 97.98 117.55 120.29
Intensity Change Log 2 — 0.252817 0.672913 0.482775 — 0.059050 0.313913 0.261979 — 0.152930 0.492984 0.369471

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000034 0.000332 0.001001 0.001989 0.000037 0.000319 0.000299 0.000641 0.000036 0.000325 0.000434 0.001666
Bud Neck 0.004953 0.008318 0.004175 0.016704 0.002669 0.012689 0.006377 0.006572 0.003573 0.010672 0.005954 0.014280
Bud Site 0.000746 0.002273 0.006326 0.022598 0.000800 0.009887 0.001990 0.024711 0.000779 0.006374 0.002822 0.023104
Cell Periphery 0.000211 0.000093 0.000096 0.000201 0.000086 0.000273 0.000065 0.000282 0.000136 0.000190 0.000071 0.000220
Cytoplasm 0.353851* 0.134953* 0.015421 0.087068 0.212620* 0.184504* 0.021279 0.067263 0.268509* 0.161641* 0.020155 0.082330
Cytoplasmic Foci 0.106949 0.016241 0.002471 0.012487 0.024648 0.036757 0.000251 0.004609 0.057217 0.027291 0.000677 0.010602
Eisosomes 0.000103 0.000030 0.000044 0.000034 0.000026 0.000050 0.000018 0.000037 0.000056 0.000041 0.000023 0.000035
Endoplasmic Reticulum 0.000559 0.001749 0.011942 0.006178 0.000668 0.001855 0.005055 0.007670 0.000625 0.001806 0.006377 0.006535
Endosome 0.003000 0.001508 0.001333 0.017171 0.000930 0.004939 0.000423 0.008963 0.001749 0.003356 0.000597 0.015207
Golgi 0.000736 0.000613 0.002774 0.007722 0.000136 0.000919 0.000030 0.003993 0.000374 0.000778 0.000556 0.006830
Lipid Particles 0.008149 0.001891 0.000615 0.001129 0.001976 0.003814 0.000246 0.000567 0.004419 0.002927 0.000317 0.000995
Mitochondria 0.003355 0.003597 0.000828 0.010549 0.001796 0.009844 0.000860 0.007063 0.002413 0.006962 0.000854 0.009715
Mitotic Spindle 0.000471 0.007408 0.000796 0.065997 0.001190 0.015812 0.001084 0.016170 0.000905 0.011934 0.001029 0.054076
None 0.015657 0.008482 0.001673 0.011924 0.008051 0.008429 0.003767 0.028943 0.011061 0.008453 0.003365 0.015996
Nuclear Periphery 0.000914 0.001158 0.001073 0.003385 0.001038 0.002183 0.001040 0.001544 0.000989 0.001710 0.001046 0.002944
Nuclear Periphery Foci 0.000391 0.000262 0.000444 0.003200 0.000191 0.000587 0.000170 0.000766 0.000271 0.000437 0.000222 0.002618
Nucleolus 0.002579 0.002688 0.001554 0.001336 0.001551 0.005659 0.000828 0.001696 0.001958 0.004288 0.000967 0.001423
Nucleus 0.479651* 0.798406* 0.936101* 0.682711* 0.735563* 0.677013* 0.950782* 0.783065* 0.634292* 0.733024* 0.947965* 0.706721*
Peroxisomes 0.003668 0.002040 0.000225 0.002476 0.000923 0.004127 0.000078 0.000998 0.002009 0.003164 0.000106 0.002123
Vacuole 0.013696 0.007408 0.010966 0.037233 0.004913 0.018732 0.005245 0.028243 0.008388 0.013507 0.006343 0.035082
Vacuole Periphery 0.000325 0.000550 0.000140 0.007906 0.000189 0.001609 0.000114 0.006205 0.000243 0.001120 0.000119 0.007499

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.21 -11.40 -7.28 -4.17 -0.14 -4.37 -14.17 -11.54 -6.02 -6.70 -4.12 -17.60 -8.99 -5.69 -4.69
Bud Neck -2.68 -0.40 -7.23 -3.92 -7.79 -8.46 -3.87 -7.77 2.35 -1.98 -8.20 -3.35 -10.12 -2.32 -5.61
Bud Site -2.39 -1.33 -7.26 -5.76 -2.01 -8.06 -2.73 -3.16 -0.52 -2.75 -8.22 -2.69 -7.69 -3.41 -5.75
Cell Periphery 6.81 5.86 3.24 -4.36 -3.15 -9.20 2.55 -3.10 2.62 -3.59 -4.44 7.62 -1.95 2.25 -7.15
Cytoplasm 25.82 42.99 36.93 11.15 -10.01 3.72 34.88 20.09 16.92 -5.43 18.83 53.89 38.46 20.93 -13.18
Cytoplasmic Foci 19.74 23.69 23.61 8.12 -1.68 -4.80 16.14 12.70 15.10 -1.91 11.76 27.42 25.06 16.68 -4.73
Eisosomes 10.83 8.17 9.32 -4.69 -0.42 -9.13 0.84 -8.86 -3.09 -9.25 4.45 10.45 -0.28 -5.07 -12.33
Endoplasmic Reticulum -6.88 -11.09 -23.13 -18.41 3.01 -7.66 -17.58 -14.41 -12.82 -7.10 -10.21 -20.19 -27.12 -22.82 -5.57
Endosome 3.50 3.19 -2.70 -4.32 -4.32 -6.67 2.43 -2.02 0.60 -2.30 -4.32 5.50 -4.32 -2.21 -5.72
Golgi 0.32 -0.60 -1.88 -2.21 -0.23 -3.71 1.69 -1.36 -0.47 -1.47 -1.94 0.00 -2.79 -2.05 -2.51
Lipid Particles 7.73 10.52 10.12 2.42 -2.42 -4.65 8.80 5.42 8.35 -7.00 3.51 13.31 11.12 7.61 -8.78
Mitochondria -0.25 2.85 -1.14 -1.01 -4.18 -5.96 1.56 -1.20 3.42 -1.88 -5.26 3.11 -2.32 2.40 -4.59
Mitotic Spindle -3.86 -1.25 -9.65 -7.33 -9.40 -6.46 -0.76 -3.80 -0.80 -3.62 -7.45 -1.18 -10.26 -6.33 -9.82
None 3.16 6.72 3.71 0.69 -6.12 -0.40 3.91 -1.80 -1.65 -3.15 2.03 7.14 1.32 -0.47 -5.20
Nuclear Periphery -2.19 -2.98 -11.20 -10.84 -10.01 -5.04 -5.04 -9.57 -3.60 -8.00 -5.51 -5.59 -13.39 -9.57 -11.83
Nuclear Periphery Foci 1.33 -1.39 -2.45 -2.66 -2.06 -3.10 -1.10 -4.93 -2.34 -4.58 -2.38 -0.87 -3.21 -2.68 -3.08
Nucleolus 0.12 1.57 2.49 4.16 0.81 -5.65 2.69 -0.06 5.49 -2.18 -4.98 3.39 2.27 6.68 -1.81
Nucleus -30.70* -40.83 -23.65 4.12 15.61 6.61 -30.25 -4.00 -7.98 10.70 -12.88 -50.11 -14.50 -3.85 22.97
Peroxisomes 1.84 4.57 3.33 2.35 -1.80 -5.67 3.46 1.74 6.90 -1.97 -2.69 5.72 2.87 5.76 -2.36
Vacuole 3.98 -1.24 -13.02 -14.48 -10.84 -10.14 -4.46 -8.26 -5.93 -7.38 -6.29 -2.76 -16.57 -14.44 -15.14
Vacuole Periphery -1.72 3.97 -3.02 -2.65 -3.29 -4.84 2.05 -2.14 -0.63 -2.22 -5.19 4.00 -3.73 -2.05 -3.96
Standard name
Human Ortholog
Description 3'-5' DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; acts with Rad4p in nucleotide-excision repair; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome (RTS)
Localization
Cell Percentages nucleus (40%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Hrq1

Hrq1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hrq1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available