Standard name | |
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Human Ortholog | |
Description | 3'-5' DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; acts with Rad4p in nucleotide-excision repair; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome (RTS) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Bud | 0 | 0 | 1 | – | 1 | 0 | 3 | 4 | 5 | – | 3 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 3 | 5 | 6 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | – | 0 | 2 | 3 | 8 | 3 | – | 4 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 25 | 6 | 4 | – | 34 | 49 | 61 | 63 | 36 | – | 17 | 40 | 61 | 82 | 105 | 25 | 12 | 25 | 9 | 4 | 10 | 17 | 45 | 31 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 8 | 5 | 9 | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 1 | 0 | 0 | – | 1 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 4 | 1 | 1 | 4 | 3 | 1 | 1 | 0 | 1 | 2 | 5 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 1 | 52 | 12 | – | 46 | 83 | 142 | 165 | 119 | – | 126 | 162 | 34 | 28 | 73 | 13 | 6 | 16 | 3 | 1 | 4 | 1 | 8 | 13 |
Nucleus | 123 | 60 | 11 | – | 45 | 48 | 76 | 77 | 58 | – | 52 | 75 | 118 | 151 | 93 | 87 | 81 | 92 | 129 | 89 | 27 | 98 | 117 | 121 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | – | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | – | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 1 | – | 1 | 3 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 5 | 10 | 15 |
Vac/Vac Membrane | 30 | 32 | 0 | – | 17 | 7 | 22 | 12 | 7 | – | 4 | 9 | 39 | 42 | 49 | 35 | 25 | 30 | 37 | 21 | 29 | 48 | 95 | 120 |
Unique Cell Count | 163 | 109 | 22 | 117 | 146 | 231 | 245 | 161 | 153 | 210 | 224 | 276 | 276 | 149 | 122 | 151 | 187 | 122 | 80 | 181 | 297 | 334 | ||
Labelled Cell Count | 180 | 151 | 28 | 145 | 192 | 308 | 329 | 231 | 211 | 300 | 254 | 308 | 324 | 172 | 135 | 177 | 187 | 122 | 80 | 181 | 297 | 334 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 3.6 | 3.7 | – | 3.8 | 3.6 | 3.6 | 3.4 | 3.3 | – | 2.8 | 2.7 | 4.1 | 4.4 | 3.8 | 5.8 | 6.6 | 6.5 | 4.6 | 4.1 | 5.0 |
Std Deviation (1e-4) | 0.5 | 0.6 | 1.4 | – | 1.5 | 1.7 | 1.9 | 1.7 | 2.3 | – | 1.2 | 1.2 | 0.8 | 0.9 | 0.9 | 1.6 | 1.8 | 1.9 | 1.5 | 1.5 | 1.4 |
Intensity Change (Log2) | – | – | – | – | 0.03 | -0.06 | -0.05 | -0.12 | -0.19 | – | -0.4 | -0.47 | 0.13 | 0.22 | 0.03 | 0.64 | 0.82 | 0.79 | 0.3 | 0.14 | 0.41 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0433 | 0.9713 | 0.8503 | 1.0191 | 0.4533 | 1.0516 | 0.9538 | 1.7275 | 1.3957 | 1.3917 | 1.8515 | 1.3543 | 0.7858 | 2.1858 | 1.3144 | 2.2363 | 1.0714 | 1.2594 |
Actin | 0.0729 | 0.0002 | 0.0051 | 0.0002 | 0.0118 | 0.0038 | 0.0316 | 0.0005 | 0.0065 | 0.0087 | 0.0016 | 0.0058 | 0.0011 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Bud | 0.0013 | 0.0003 | 0.0002 | 0.0004 | 0.0008 | 0.0001 | 0.0009 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 |
Bud Neck | 0.0025 | 0.0009 | 0.0012 | 0.0005 | 0.0021 | 0.0214 | 0.0034 | 0.0003 | 0.0009 | 0.0007 | 0.0008 | 0.0048 | 0.0004 | 0.0001 | 0.0002 | 0.0005 | 0.0004 | 0.0023 |
Bud Periphery | 0.0017 | 0.0003 | 0.0005 | 0.0003 | 0.0018 | 0.0003 | 0.0011 | 0 | 0.0007 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 |
Bud Site | 0.0045 | 0.0024 | 0.0011 | 0.0003 | 0.0019 | 0.0004 | 0.006 | 0.0121 | 0.0092 | 0.001 | 0.0002 | 0.0002 | 0.001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Cell Periphery | 0.0008 | 0.0001 | 0.0004 | 0 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.053 | 0.0849 | 0.048 | 0.0328 | 0.0318 | 0.0377 | 0.0334 | 0.0423 | 0.034 | 0.0235 | 0.0212 | 0.0127 | 0.0219 | 0.0541 | 0.0396 | 0.0281 | 0.0365 | 0.0192 |
Cytoplasmic Foci | 0.0428 | 0.0083 | 0.0041 | 0.0093 | 0.0092 | 0.0147 | 0.0229 | 0.001 | 0.0059 | 0.0301 | 0.0181 | 0.0029 | 0.0064 | 0.0003 | 0.0007 | 0.0008 | 0.0004 | 0.0006 |
Eisosomes | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0067 | 0.0031 | 0.0046 | 0.0011 | 0.0021 | 0.0023 | 0.0033 | 0.0012 | 0.0014 | 0.0008 | 0.0013 | 0.0006 | 0.0011 | 0.0069 | 0.0011 | 0.0007 | 0.0019 | 0.0007 |
Endosome | 0.0184 | 0.0078 | 0.0065 | 0.0545 | 0.0214 | 0.0052 | 0.0145 | 0.0006 | 0.0056 | 0.0299 | 0.0258 | 0.0042 | 0.0146 | 0.0011 | 0.0008 | 0.0011 | 0.0007 | 0.0008 |
Golgi | 0.0077 | 0.0017 | 0.004 | 0.0098 | 0.0143 | 0.0019 | 0.0077 | 0.0001 | 0.0025 | 0.0045 | 0.0097 | 0.0045 | 0.002 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0276 | 0.0024 | 0.0068 | 0.0003 | 0.0344 | 0.004 | 0.0295 | 0.0004 | 0.0076 | 0.0043 | 0.0626 | 0.0113 | 0.0107 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 |
Mitochondria | 0.0067 | 0.0011 | 0.0134 | 0.007 | 0.0869 | 0.0097 | 0.0094 | 0.0003 | 0.0041 | 0.0024 | 0.0064 | 0.0106 | 0.0008 | 0.0004 | 0.0004 | 0.0011 | 0.0003 | 0.0002 |
None | 0.0469 | 0.1025 | 0.0469 | 0.0945 | 0.0604 | 0.0168 | 0.0349 | 0.1188 | 0.0626 | 0.0182 | 0.0571 | 0.0328 | 0.0796 | 0.1439 | 0.2055 | 0.2207 | 0.1589 | 0.0872 |
Nuclear Periphery | 0.0379 | 0.0156 | 0.0183 | 0.0081 | 0.0648 | 0.0342 | 0.0129 | 0.009 | 0.0134 | 0.0123 | 0.046 | 0.0087 | 0.0052 | 0.0346 | 0.0092 | 0.0146 | 0.0089 | 0.0046 |
Nucleolus | 0.0107 | 0.005 | 0.0035 | 0.0055 | 0.0274 | 0.0088 | 0.0044 | 0.0032 | 0.0046 | 0.0032 | 0.0042 | 0.0067 | 0.004 | 0.0016 | 0.0026 | 0.0074 | 0.0058 | 0.0069 |
Nucleus | 0.5951 | 0.7509 | 0.8121 | 0.6996 | 0.5858 | 0.7992 | 0.7163 | 0.7987 | 0.8194 | 0.8208 | 0.6461 | 0.8613 | 0.835 | 0.7488 | 0.7318 | 0.6534 | 0.7724 | 0.8626 |
Peroxisomes | 0.0197 | 0.0008 | 0.0117 | 0.0002 | 0.0057 | 0.0037 | 0.0312 | 0.0001 | 0.0084 | 0.0196 | 0.0545 | 0.0076 | 0.0014 | 0 | 0 | 0.0001 | 0 | 0 |
Punctate Nuclear | 0.0261 | 0.0084 | 0.0058 | 0.07 | 0.0284 | 0.0328 | 0.0324 | 0.0106 | 0.0091 | 0.0132 | 0.0403 | 0.0238 | 0.0119 | 0.0055 | 0.0063 | 0.0695 | 0.0128 | 0.014 |
Vacuole | 0.012 | 0.0029 | 0.004 | 0.0027 | 0.0043 | 0.0014 | 0.0026 | 0.0005 | 0.0026 | 0.005 | 0.0015 | 0.0004 | 0.0019 | 0.0017 | 0.0009 | 0.0008 | 0.0005 | 0.0004 |
Vacuole Periphery | 0.0045 | 0.0004 | 0.0016 | 0.0027 | 0.0037 | 0.0012 | 0.0008 | 0.0001 | 0.0005 | 0.0013 | 0.0021 | 0.0004 | 0.0005 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.1358 | 13.4201 | 13.5795 | 15.1425 | 10.838 | 8.184 | 15.4194 | 14.0495 | 14.8847 | 16.1258 |
Translational Efficiency | 0.355 | 0.3826 | 0.3403 | 0.3285 | 0.4549 | 0.4986 | 0.3194 | 0.3159 | 0.3465 | 0.334 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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